CN103575851B - Method for rapidly evaluating usage amount of silica gel and PVPP (Crosslinked Polyethylene Pyrrolidone) by using charge titration technique - Google Patents
Method for rapidly evaluating usage amount of silica gel and PVPP (Crosslinked Polyethylene Pyrrolidone) by using charge titration technique Download PDFInfo
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- CN103575851B CN103575851B CN201310522557.7A CN201310522557A CN103575851B CN 103575851 B CN103575851 B CN 103575851B CN 201310522557 A CN201310522557 A CN 201310522557A CN 103575851 B CN103575851 B CN 103575851B
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- pvpp
- silica gel
- beer
- charges
- filtered
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 239000000741 silica gel Substances 0.000 title claims abstract description 44
- 229910002027 silica gel Inorganic materials 0.000 title claims abstract description 44
- 235000013809 polyvinylpolypyrrolidone Nutrition 0.000 title claims abstract description 41
- 229920000523 polyvinylpolypyrrolidone Polymers 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000004448 titration Methods 0.000 title claims abstract description 10
- 229920003020 cross-linked polyethylene Polymers 0.000 title abstract 2
- 239000004703 cross-linked polyethylene Substances 0.000 title abstract 2
- 235000013405 beer Nutrition 0.000 claims abstract description 33
- 238000005516 engineering process Methods 0.000 claims description 9
- 238000011156 evaluation Methods 0.000 claims description 9
- 238000004458 analytical method Methods 0.000 claims description 7
- 150000008442 polyphenolic compounds Chemical class 0.000 abstract description 13
- 235000013824 polyphenols Nutrition 0.000 abstract description 13
- 102000004169 proteins and genes Human genes 0.000 abstract description 12
- 108090000623 proteins and genes Proteins 0.000 abstract description 12
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000001914 filtration Methods 0.000 abstract description 3
- 230000000087 stabilizing effect Effects 0.000 abstract 4
- 239000002245 particle Substances 0.000 abstract 3
- 239000007787 solid Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000005728 strengthening Methods 0.000 description 3
- 239000002671 adjuvant Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 230000032683 aging Effects 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
Landscapes
- Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
Abstract
The invention relates to a method for evaluating the application of a stabilizing auxiliary in a beer filtration process, and in particular relates to a method for rapidly evaluating the usage amount of silica gel and PVPP (Crosslinked Polyethylene Pyrrolidone) by using a charge titration technique. Charges of colloidal particles of sensitive protein and sensitive polyphenol in beer are negative, the charges of silica gel and PVPP are positive, and along with the increase of the usage amount of the silica gel and the PVPP, the charges of the colloidal particles in the beer are gradually converted into positive charges from negative charges. The addition amount of the silica gel and the PVPP of which the number of the charges is approximate to 0 is taken as the optimal usage amount of the silica gel and the PVPP. According to the method, by evaluating the charge situation of the colloidal particles of the silica gel and the PVPP of the beer, the optimal action condition and optimal usage proportion of the beer stabilizing auxiliaries can be effectively determined. Therefore, the application of the stabilizing auxiliary can be rapidly optimized, and the purposes of stabilizing the quality of the beer and lowering the production cost are achieved.
Description
Technical field
The present invention relates to the method that the stabilization aid in evaluating beer filter process uses, relate to a kind of method utilizing charge titration technology Fast Evaluation silica gel and PVPP use amount.
Background technology
Sensitive Protein and sensitive polyphenols are the materials affecting beer colloidal stability.Silica gel and PVPP are stability auxiliary agent, and silica gel is for removing the sensitive Protein in beer, and PVPP is for removing the sensitive polyphenols in beer.The use amount of silica gel and PVPP determines the effect that it removes sensitive Protein and sensitive polyphenols.Addition is little can cause sensitive Protein and sensitive polyphenols to remove thoroughly, affects the colloidal stability of beer thus the shelf life of shortening beer; And large albumen and the polyphenols of causing of addition is removed too much, affect foam and the oxidation resistance of beer, cause the increase of production cost simultaneously.
Judgement at present for the use amount of silica gel and PVPP mainly carries out strengthening experiment to the wine liquid processed, and at high temperature accelerates the aging colloidal stability evaluating wine liquid of beer, or the index of correlation of mensuration wine liquid, such as sensitive Protein and sensitive polyphenols.The former is the method that actual production adopts, and shortcoming is that test period is long, needs for 1 to 2 weeks, there is hysteresis quality.The latter utilizes indirect indexes to evaluate, and accuracy is not high.All there is hysteresis quality in two kinds of methods, cannot judge the use amount of silica gel and PVPP in advance.
Summary of the invention
The present invention is directed to deficiency of the prior art, electrically charged on the contrary electrically charged with the stabilization aid such as silica gel, PVPP institute by the colloidal solid institute of the sensitive Protein in test discovery beer and sensitive polyphenols, thus set up a set of method utilizing charge titration technology Fast Evaluation silica gel and PVPP use amount.
Colloidal solid institute in beer is electrically charged be negative, and silica gel and PVPP (crospolyvinylpyrrolidone) electrically charged be just.Along with the increase of silica gel and PVPP use amount, the colloidal solid electric charge in wine can progressively be changed to positive charge by negative charge.Utilize electric charge level off to 0 time silica gel and the addition of PVPP as the optimum quantum of utilization of silica gel and PVPP.
Charge analysis instrument is adopted to detect the electrically charged situation of colloidal solid of adding beer before and after silica gel, PVPP.
Technical scheme of the present invention is:
Utilize a method for charge titration technology Fast Evaluation silica gel and PVPP use amount, the step of described method is as follows:
Step 1: wine to be filtered is added charge analysis instrument and measures its streaming potential V;
Step 2: take silica gel or PVPP is for subsequent use;
Step 3: after streaming potential V is stable, the silica gel taken in interpolation step 2 slowly, on a small quantity, repeatedly toward wine to be filtered in or PVPP, stop interpolation when treating that streaming potential V reaches at 0;
Step 4: record is the weight Ws of remaining silica gel or the weight Wp of residue PVPP now;
Step 5: silica gel use amount D1=(the 1-Ws)/Lp determining wine the best to be filtered;
Step 6: PVPP use amount D2=(the 1-Wp)/Lp determining wine the best to be filtered.
Preferably, the wine to be filtered described in step 1 is beer, pH3.5-6.5.
The invention has the beneficial effects as follows:
(1) the present invention by evaluating beer and silica gel, PVPP the electrically charged situation of colloidal solid, utilize the principle that positive and negative charge attracts mutually, effectively can determine the action condition of beer and filtration adjuvant the best and bestly use proportioning.Thus rapid Optimum is carried out to the use of filtration adjuvant.Reach the quality of stable beer and reduce the object of production cost.
(2) the present invention compares strengthening experiment and sensitive Protein, sensitive polyphenols determination method is more quick, easy, can at 3 hours with the result of use of inner evaluation silica gel and PVPP, and the time in method 1 to 2 weeks of needs of traditional strengthening experiment.
(3) the present invention in time, accurately can adjust the use amount of silica gel and PVPP, avoids the excessive interpolation that the difference of wine fluidity matter causes, thus reduces production cost.
Embodiment
The specific embodiment of the present invention is as follows:
Example 1:
Utilize a method for charge titration technology Fast Evaluation silica gel use amount, the step of described method is as follows:
Step 1: beer to be filtered for 10ml is added charge analysis instrument and measures its streaming potential V; Detected by charge analysis instrument, its streaming potential is-100mv;
Step 2: take 1g silica gel for subsequent use;
Step 3: after streaming potential V is stable, the silica gel taken in interpolation step 2 slowly, on a small quantity, repeatedly toward beer to be filtered in, the streaming potential of beer to be filtered rises gradually, stops interpolation when treating that streaming potential V reaches at 0;
Step 4: the weight Ws recording now remaining silica gel is 0.996g;
Step 5: silica gel use amount D1=(1-Ws)/Lp=(the 1g-0.996g)/10ml=400mg/l determining beer the best to be filtered;
Now the use amount of consumption and manufacturer recommends is similar.
Example 2:
Utilize a method for charge titration technology Fast Evaluation PPVP use amount, the step of described method is as follows:
Step 1: beer to be filtered for 10ml is added charge analysis instrument and measures its streaming potential V; Detected by charge analysis instrument, its streaming potential is-100mv;
Step 2: take 1g PPVP for subsequent use;
Step 3: after streaming potential V is stable, the PPVP taken in interpolation step 2 slowly, on a small quantity, repeatedly toward beer to be filtered in, the streaming potential of beer to be filtered rises gradually, stops interpolation when treating that streaming potential V reaches at 0;
Step 4: the weight Ws recording now remaining PVPP is 0.998g;
Step 5: PVPP use amount D1=(1-Ws)/Lp=(the 1g-0.998g)/10ml=200mg/l determining beer the best to be filtered;
Now the use amount of consumption and manufacturer recommends is similar.
Experimental example:
Determine the colloid streaming potential V of the beer within the scope of pH4.0 and pH4.4, sensitive Protein, sensitive polyphenols, silica gel solution, PVPP solution.
| PH4.0 streaming potential mv | PH4.4 streaming potential mv | |
| Beer | -220 | -340 |
| Sensitive Protein | -124 | -243 |
| Sensitive polyphenols | -115 | -189 |
| Silica gel | 890 | 440 |
| PVPP | 1120 | 970 |
As can be seen from the results, in the scope of pH4.0-4.4, the colloidal solid electric charge of beer is negative, and this is that the colloidal solid electric charge of the material such as sensitive Protein, sensitive polyphenols owing to wherein containing is caused for bearing.And the colloidal solid electric charge of silica gel and PVPP is just.Mutual suction-operated with silica gel and sensitive Protein, PVPP and sensitive polyphenols is described.Both differences are larger, and suction-operated is stronger.
Claims (2)
1. utilize a method for charge titration technology Fast Evaluation silica gel or PVPP use amount, it is characterized in that, the step of described method is as follows:
Step 1: wine Lp to be filtered is added charge analysis instrument and measures its streaming potential V;
Step 2: take 1g silica gel or PVPP for subsequent use;
Step 3: after streaming potential V is stable, the silica gel taken in interpolation step 2 slowly, on a small quantity, repeatedly toward wine to be filtered in or PVPP, stop interpolation when treating that streaming potential V reaches at 0;
Step 4: record is the weight Ws of remaining silica gel or the weight Wp of residue PVPP now;
Step 5: silica gel use amount D1=(the 1-Ws)/Lp determining wine the best to be filtered;
Step 6: PVPP use amount D2=(the 1-Wp)/Lp determining wine the best to be filtered.
2. the method utilizing charge titration technology Fast Evaluation silica gel or PVPP use amount according to claim 1, is characterized in that the wine to be filtered described in step 1 is beer, pH3.5-6.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310522557.7A CN103575851B (en) | 2013-10-29 | 2013-10-29 | Method for rapidly evaluating usage amount of silica gel and PVPP (Crosslinked Polyethylene Pyrrolidone) by using charge titration technique |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310522557.7A CN103575851B (en) | 2013-10-29 | 2013-10-29 | Method for rapidly evaluating usage amount of silica gel and PVPP (Crosslinked Polyethylene Pyrrolidone) by using charge titration technique |
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| Publication Number | Publication Date |
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| CN103575851A CN103575851A (en) | 2014-02-12 |
| CN103575851B true CN103575851B (en) | 2015-02-18 |
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| CN201310522557.7A Active CN103575851B (en) | 2013-10-29 | 2013-10-29 | Method for rapidly evaluating usage amount of silica gel and PVPP (Crosslinked Polyethylene Pyrrolidone) by using charge titration technique |
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Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10051266A1 (en) * | 2000-10-16 | 2002-04-25 | Basf Ag | Filter aid used for filtering fruit and fermented drinks comprising polystyrene and silicate, carbonate, oxide, silica gel, diatomaceous earth and/or polymers |
| US20030158124A1 (en) * | 2002-02-20 | 2003-08-21 | Isp Investments Inc. | Functionalized tannin and improved protein extractant |
| CN1325904C (en) * | 2002-11-28 | 2007-07-11 | 华南理工大学 | Photometry of paper pulp suspension liquid colloid dissolving electric charge |
| KR101197033B1 (en) * | 2004-05-18 | 2012-11-06 | 앤호이저-부쉬 인베브 에스.에이. | Method of preparing a liquid, containing proteins for subsequent separation, by using one or more protein-complexing agents |
| CN101544933A (en) * | 2009-05-18 | 2009-09-30 | 北京恒昌盛德科技发展有限公司 | Formaldehyde free brewage art |
| WO2012051466A1 (en) * | 2010-10-14 | 2012-04-19 | Sportsman Consulting Llc | Analytical method and titration device |
| EP2515098B1 (en) * | 2011-04-18 | 2019-04-03 | Büchi Labortechnik AG | Method and device for determining the SO2 content of food and drink |
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Address after: 266000 Shandong city of Qingdao Province North Dengzhou Road No. 56 Applicant after: Qingdao Beer Co., Ltd. Address before: Shinan District 266000 in Shandong province Qingdao City 54 square building in Tsingtao Applicant before: Qingdao Beer Co., Ltd. |
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