CN103575840A - Method for detecting content of formaldehyde in milk - Google Patents
Method for detecting content of formaldehyde in milk Download PDFInfo
- Publication number
- CN103575840A CN103575840A CN201210255152.7A CN201210255152A CN103575840A CN 103575840 A CN103575840 A CN 103575840A CN 201210255152 A CN201210255152 A CN 201210255152A CN 103575840 A CN103575840 A CN 103575840A
- Authority
- CN
- China
- Prior art keywords
- formaldehyde
- solution
- standard
- sample
- milk
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Dairy Products (AREA)
Abstract
The invention provides a method for detecting the content of formaldehyde in milk. The method comprises the following steps of: preparing standard solution and 2,4-dinitrophenylhydrazine solution; adding 2,4-dinitrophenylhydrazine solution in each standard solution and uniformly shaking to obtain standard reaction solution; taking the to-be-detected milk, adding 2,4-dinitrophenylhydrazine solution and uniformly shaking to obtain sample reaction solution; reacting the standard reaction solution and the sample reaction solution for 9-11 minutes in a water bath at 58-62 DEG C, adding n-hexane after cooling, performing volution for 50-70 seconds and then centrifugally layering to obtain standard centrifugal solution and sample centrifugal solution; filtering the supernatant solution of the standard centrifugal solution and the sample centrifugal solution, and detecting the gas chromatographic value of the supernatant solution; drawing a standard curve according to the concentration of formaldehyde in the standard solution and a gas chromatographic value corresponding to the standard solution; and calculating the content of formaldehyde in the to-be-detected milk according to the gas chromatographic value corresponding to the to-be-detected milk and the standard curve. The detection method provided by the invention is simple to operate, and the detection result is high in accuracy.
Description
Technical field
The present invention relates to a kind of method that detects formaldehyde in food content, be particularly useful for detecting the content of formaldehyde in cow's milk.
Background technology
During purchase fresh milk, some illegal retailers can add formaldehyde in milk, improve some physical and chemical index of milk with the means of cheating.In order to guarantee to purchase the quality of fresh milk, be necessary when purchase, the content of formaldehyde in a large amount of fresh milks to be detected.
At present, by vapor-phase chromatography, detect the detection method that mensuration-vapor-phase chromatography > > that formaldehyde mainly contains SNT 2611-2010 < < food contact material-woodwork Free-formaldehyde mentions.Wherein by formaldehyde, under acid condition, react with DNPH, generate stablely 2,4-dinitrophenylhydrazone, through cyclohexane extraction, goes out the free formaldehyde in woodwork with the gas chromatograph for determination of being furnished with electron waves or detecting device.When the method is detected for cow's milk content of formaldehyde, due to ox protein of milk easily with the organic solvent generation emulsification such as cyclohexane, make to extract and cannot carry out smoothly.If first will carry out subsequent detection after cow's milk deproteinized, not only step is more loaded down with trivial details again, and its deproteinized process also easily causes the loss of formaldehyde, affects the accuracy of testing result.
Summary of the invention
The object of this invention is to provide a kind of method that detects content of formaldehyde in cow's milk, it is easy and simple to handle and have a higher accuracy.
The invention provides a kind of method that detects content of formaldehyde in cow's milk, comprising: the standard solution of preparing different concentration of formaldehyde; The acetonitrile solution of preparation DNPH, obtains DNPH solution; In each standard solution, add respectively DNPH solution and shake up, obtaining the standard reaction liquid corresponding to different concentration of formaldehyde; Get cow's milk to be measured, add DNPH solution and shake up, obtain sample reactant liquor; The water-bath that each standard reaction liquid and sample reactant liquor are put into 58 to 62 ℃ is reacted 9 to 11 minutes, in each standard reaction liquid and sample reactant liquor, add normal hexane respectively after cooling, vortex centrifugal layering after 50 to 70 seconds, obtains standard centrifugate and sample centrifugate corresponding to different concentration of formaldehyde; The supernatant liquor that filters each standard centrifugate and sample centrifugate obtains standard supernatant and the sample supernatant corresponding to different concentration of formaldehyde, detects the gas chromatography value of each standard supernatant and sample supernatant; According to the concentration of formaldehyde in each standard solution and the gas chromatography value drafting corresponding with each standard solution, obtain typical curve; According to gas chromatography value corresponding to cow's milk to be measured and typical curve, calculate the content of formaldehyde in cow's milk to be measured.Wherein, the compound method of DNPH solution is preferably: weigh 0.05g DNPH in small beaker, with acetonitrile, dissolve and be settled to 100mL.
In a kind of another kind of exemplary embodiment of the method that detects formaldehyde in food content, process every milliliter of standard solution, needing to use the amount of DNPH solution is 0.5mL; Process every gram of cow's milk to be measured, amount that need to use DNPH solution is 0.4 to 0.6mL.
In another exemplary embodiment of a kind of method that detects formaldehyde in food content, the condition of gas chromatographic detection is: chromatographic column is VF-5 capillary column, and specification is column length 30m, internal diameter 0.25mm, smearing thickness 0.25 μ m; Injector temperature is 240 ℃; Sample size is 1 μ L, Splitless injecting samples; Post case temperature is 140 ℃ of initial temperature, with the speed of 20 ℃/min, is warming up to 280 ℃ and maintain 8min; The temperature of electron capture detector is 300 ℃; Carrier gas is nitrogen, and flow velocity is 1.0mL/min.
In another exemplary embodiment of a kind of method that detects formaldehyde in food content, in standard solution, the concentration of formaldehyde is respectively: 0.0ng/mL, 25ng/mL, 50ng/mL, 100ng/mL and 200ng/mL.
A kind of method that detects content of formaldehyde in cow's milk provided by the invention, adopts DNPH solution-treated cow's milk and the standard solution of acetonitrile preparation, then with n-hexane extraction and carry out gas chromatographic detection.Detection method of the present invention adds DNPH again and carries out subsequent detection after cow's milk being removed to albumen, and it is simple to operate, and testing result has higher accuracy.
Embodiment
For technical characterictic, object and the effect of invention are had more clearly and understood, the existing the specific embodiment of the present invention that illustrates with the following Examples.
the first embodiment.
The gas chromatograph of selecting in the present embodiment is Varian GC-450 gas chromatograph.Wherein GC conditions is set to: chromatographic column is VF-5 capillary column, and specification is column length 30m, internal diameter 0.25mm, thickness 0.25 μ m; Injector temperature is 240 ℃; Sample size 1uL, Splitless injecting samples; Post case temperature is: 140 ℃ of initial temperature, are warming up to 280 ℃ and keep 8min with the speed of 20 ℃/min; Electron capture detector temperature is 300 ℃; Carrier gas is nitrogen, and flow velocity is 1.0mL/min; Complemental air flow velocity is 25mL/min.
Operation steps:
A. the standard solution of preparing different concentration of formaldehyde, is respectively the formalin that water that content of formaldehyde is 0.0ng/mL and content of formaldehyde are respectively 25ng/mL, 50ng/mL, 100ng/mL, 200ng/mL;
B. weigh 0.05g DNPH in small beaker, with acetonitrile, dissolve and be settled to 100mL, obtain DNPH solution;
C. get respectively each standard solution 2.0mL, add separately respectively DNPH solution 1.0mL and shake up, obtain the standard reaction liquid corresponding to different concentration of formaldehyde;
D. get cow's milk 2.0g to be measured, add DNPH solution 1.0mL and shake up, obtain sample reactant liquor;
The water-bath of e. each standard reaction liquid and sample reactant liquor being put into 60 ℃ is reacted 10 minutes, in each standard reaction liquid and sample reactant liquor, add 2.0mL normal hexane respectively after cooling, vortex is centrifugal layering after 60 seconds, obtains standard centrifugate and sample centrifugate corresponding to different concentration of formaldehyde;
F. filter the supernatant liquor of each standard centrifugate and sample centrifugate, obtain standard supernatant and sample supernatant corresponding to different concentration of formaldehyde, detect the gas chromatography value of each standard supernatant and sample supernatant;
G. according to the concentration of formaldehyde in each standard solution and the gas chromatography value drafting corresponding with each standard solution, obtain typical curve;
H. the content that calculates formaldehyde in cow's milk to be measured according to the gas chromatography value corresponding with cow's milk to be measured and typical curve.
Recovery test.
In cow's milk sample, add the different formaldehyde of measuring, make the concentration increment of its formaldehyde reach respectively 25ng/g, 50ng/g and 100ng/g, make respectively mark-on sample 1, mark-on sample 2 and mark-on sample 3.Mark-on sample 1, mark-on sample 2 and mark-on sample 3 are carried out to the detection of content of formaldehyde according to detection method described in the present embodiment, each contents level carries out respectively revision test 6 times, and test findings and the calculating gained recovery are as shown in the table.
| Measure number of times | Background values (ng/g) | Mark-on sample 1 test findings (ng/g) | Mark-on sample 2 test findings (ng/g) | Mark-on sample 3 test findings (ng/g) |
| 1 | 10.7 | 33.0 | 60.3 | 120.3 |
| 2 | 10.8 | 34.8 | 59.5 | 110.7 |
| 3 | 10.2 | 35.9 | 56.2 | 116.5 |
| 4 | 10.8 | 32.7 | 62.8 | 119.6 |
| 5 | 11.4 | 36.2 | 63.1 | 121.1 |
| 6 | 11.3 | 33.8 | 58.8 | 109.8 |
| Mean value | 10.9 | 34.4 | 60.1 | 116.3 |
| Average recovery rate | / | 94.0% | 98.4% | 105.4% |
As shown above, the average recovery rate of the mark-on sample of different content of formaldehyde is 94.0%-105.4%.Illustrate that the method can detect the content of formaldehyde in cow's milk quickly and accurately.
the second embodiment.
The gas chromatograph that the present embodiment is selected and GC conditions are with identical described in the first embodiment.
Operation steps:
A. the standard solution of preparing different concentration of formaldehyde, the water that to be respectively as content of formaldehyde be 0.0ng/mL and content of formaldehyde be the formalin of 25ng/mL, 50ng/mL, 100ng/mL, 200ng/mL respectively.
B. weigh 0.05g DNPH in small beaker, with acetonitrile, dissolve and be settled to 100mL, obtain DNPH solution;
C. get respectively each standard solution 2.0mL, add separately respectively DNPH solution 1.0mL and shake up, obtain the standard reaction liquid corresponding to different concentration of formaldehyde;
D. get cow's milk 2.0g to be measured, add DNPH solution 0.8mL and shake up, obtain sample reactant liquor;
The water-bath of e. each standard reaction liquid and sample reactant liquor being put into 58 ℃ is reacted 9 minutes, in each standard reaction liquid and sample reactant liquor, add 2.0mL normal hexane respectively after cooling, vortex is centrifugal layering after 50 seconds, obtains standard centrifugate and sample centrifugate corresponding to different concentration of formaldehyde;
F. filter the supernatant liquor of each standard centrifugate and sample centrifugate, obtain standard supernatant and sample supernatant corresponding to different concentration of formaldehyde, detect the gas chromatography value of each standard supernatant and sample supernatant;
G. according to the concentration of formaldehyde in each standard solution and the gas chromatography value drafting corresponding with each standard solution, obtain typical curve;
H. the content that calculates formaldehyde in cow's milk to be measured according to the gas chromatography value corresponding with cow's milk to be measured and typical curve.
Recovery test.
In cow's milk sample, add the different formaldehyde of measuring, make the concentration increment of its formaldehyde reach respectively 25ng/g, 50ng/g and 100ng/g, make respectively mark-on sample 1, mark-on sample 2 and mark-on sample 3.Mark-on sample 1, mark-on sample 2 and mark-on sample 3 are carried out to the detection of content of formaldehyde according to detection method described in the present embodiment, each contents level carries out respectively revision test 6 times, and test findings and the calculating gained recovery are as shown in the table.
| Measure number of times | Background values (ng/g) | Mark-on sample 1 test findings (ng/g) | Mark-on sample 2 test findings (ng/g) | Mark-on sample 3 test findings (ng/g) |
| 1 | 10.9 | 34.1 | 59.5 | 109.8 |
| 2 | 10.1 | 36.1 | 51.6 | 105.1 |
| 3 | 9.81 | 35.4 | 55.4 | 109.6 |
| 4 | 11.5 | 33.7 | 56.3 | 108.1 |
| 5 | 10.6 | 32.8 | 54.2 | 114.2 |
| 6 | 10.3 | 39.1 | 56.1 | 108.4 |
| Mean value | 10.5 | 35.2 | 55.5 | 109.2 |
| Average recovery rate | / | 98.9% | 91.6% | 98.7% |
As shown above, the average recovery rate of the mark-on sample of different content of formaldehyde is 91.6%-98.9%.Illustrate that the method can detect the content of formaldehyde in cow's milk quickly and accurately.
the 3rd embodiment.
The gas chromatograph that the present embodiment is selected and GC conditions are with identical described in the first embodiment.
Operation steps:
A. the standard solution of preparing different concentration of formaldehyde, the water that to be respectively as content of formaldehyde be 0.0ng/mL and content of formaldehyde be the formalin of 25ng/mL, 50ng/mL, 100ng/mL, 200ng/mL respectively;
B. weigh 0.05g DNPH in small beaker, with acetonitrile, dissolve and be settled to 100mL, obtain DNPH solution;
C. get respectively each standard solution 2.0mL, add separately respectively DNPH solution 1.0mL and shake up, obtain the standard reaction liquid corresponding to different concentration of formaldehyde;
D. get cow's milk 2.0g to be measured, add DNPH solution 1.2mL and shake up, obtain sample reactant liquor;
The water-bath of e. each standard reaction liquid and sample reactant liquor being put into 62 ℃ is reacted 11 minutes, in each standard reaction liquid and sample reactant liquor, add 2.0mL normal hexane respectively after cooling, vortex is centrifugal layering after 70 seconds, obtains standard centrifugate and sample centrifugate corresponding to different concentration of formaldehyde;
F. filter the supernatant liquor of each standard centrifugate and sample centrifugate, obtain standard supernatant and sample supernatant corresponding to different concentration of formaldehyde, detect the gas chromatography value of each standard supernatant and sample supernatant;
G. according to the concentration of formaldehyde in each standard solution and the gas chromatography value drafting corresponding with each standard solution, obtain typical curve;
H. the content that calculates formaldehyde in cow's milk to be measured according to the gas chromatography value corresponding with cow's milk to be measured and typical curve.
Recovery test.
In cow's milk sample, add the different formaldehyde of measuring, make the concentration increment of its formaldehyde reach respectively 25ng/g, 50ng/g and 100ng/g, make respectively mark-on sample 1, mark-on sample 2 and mark-on sample 3.Mark-on sample 1, mark-on sample 2 and mark-on sample 3 are carried out to the detection of content of formaldehyde according to detection method described in the present embodiment, each contents level carries out respectively revision test 6 times, and test findings and the calculating gained recovery are as shown in the table.
| Measure number of times | Background values (ng/g) | Mark-on sample 1 test findings (ng/g) | Mark-on sample 2 test findings (ng/g) | Mark-on sample 3 test findings (ng/g) |
| 1 | 10.9 | 34.2 | 59.8 | 102.3 |
| 2 | 11.2 | 33.6 | 57.2 | 104.2 |
| 3 | 10.5 | 34.8 | 58.5 | 108.3 |
| 4 | 10.4 | 36.9 | 62.3 | 107.5 |
| 5 | 11.1 | 37.2 | 61.8 | 100.4 |
| 6 | 10.2 | 32.8 | 62.4 | 100.3 |
| Mean value | 10.7 | 34.9 | 60.3 | 103.8 |
| Average recovery rate | / | 97.8% | 99.3% | 93.7% |
As shown above, the average recovery rate of the mark-on sample of different content of formaldehyde is 93.7%-99.3%.Illustrate that the method can detect the content of formaldehyde in cow's milk quickly and accurately.
contrast experiment.
1, reagent and instrument.
DNPH solution: take in the dissolving of 0.1g DNPH and 24mL concentrated hydrochloric acid, add water and be settled to 100mL.
Standard solution: for content of formaldehyde is respectively water and the formalin of 0.0ng/mL, 25ng/mL, 50ng/mL, 100ng/mL, 200ng/mL.
Mark-on sample: add the different formaldehyde of measuring in cow's milk sample, make the concentration increment of its formaldehyde reach respectively 25ng/g, 50ng/g and 100ng/g, make respectively mark-on sample 1, mark-on sample 2 and mark-on sample 3.
Gas chromatograph: Varian GC-450 gas chromatograph.Wherein GC conditions is set to: chromatographic column is VF-5 capillary column, and specification is column length 30m, internal diameter 0.25mm, thickness 0.25 μ m; Injector temperature is 240 ℃; Sample size 1uL, Splitless injecting samples; Post case temperature is: 140 ℃ of initial temperature, are warming up to 280 ℃ and keep 8min with the speed of 20 ℃/min; Electron capture detector temperature is 300 ℃; Carrier gas is nitrogen, and flow velocity is 1.0mL/min; Complemental air flow velocity is 25mL/min.
2, step:
A. get respectively each mark-on sample 5.0mL in 25mL volumetric flask, add respectively 3.0mL ferrocyanide aqueous solutions of potassium (92g/L) and 3.0mL aqueous zinc acetate solution (183g/L), and with ultrapure water, be settled to 25mL respectively, then proceed to respectively in 50mL centrifuge tube, 4000 revs/min, centrifugal 5 minutes, obtain respectively the liquid to be measured corresponding to the mark-on sample of different concentration of formaldehyde increments;
B. each 5mL of standard solution that gets respectively different content of formaldehyde is placed in 50mL centrifuge tube, add water to 10mL, add 0.3mL DNPH solution, shake up and be placed on 60 ℃ of water-baths 15 minutes, after taking-up is quick cooling in current, add 5mL cyclohexane, shake up, ultrasonic extraction 2 minutes, centrifugal 5 minutes, pipette cyclohexane in clean tube; Add 5mL cyclohexane, re-extract once, after centrifugal 5 minutes, merges cyclohexane layer again, and vortex mixes, and detects, according to the concentration drawing standard curve of formaldehyde in testing result and standard solution with gas chromatograph;
C. each 10mL of liquid to be measured getting respectively corresponding to different mark-on samples is placed in 50mL centrifuge tube, add respectively 0.3mL 2,4-dinitro benzene hydrazine solution, shake up and be placed on 60 ℃ of water-baths 15 minutes, after taking-up is quick cooling in current, add respectively 5mL cyclohexane, shake up, ultrasonic extraction 2 minutes, centrifugal 5 minutes, pipettes respectively cyclohexane layer in clean tube; Add respectively 5mL cyclohexane, re-extract once, after centrifugal 5 minutes, merges cyclohexane layer again, and vortex mixes, and detects respectively with gas chromatograph, and the typical curve obtaining according to testing result and step b), calculates the content of formaldehyde in mark-on sample.
The test findings that duplicate detection is 6 times and the calculating gained recovery are as shown in the table.
| Measure number of times | Background values (ng/g) | Mark-on sample 1 test findings (ng/g) | Mark-on sample 2 test findings (ng/g) | Mark-on sample 3 test findings (ng/g) |
| 1 | 10.3 | 34.1 | 51.2 | 98.6 |
| 2 | 10.2 | 31.2 | 58.1 | 97.5 |
| 3 | 9.91 | 30.4 | 49.5 | 101.4 |
| 4 | 10.9 | 34.1 | 48.5 | 100.6 |
| 5 | 11.1 | 31.0 | 50.8 | 99.4 |
| 6 | 11.2 | 30.9 | 53.1 | 98.7 |
| Mean value | 10.6 | 32.0 | 51.9 | 99.4 |
| Average recovery rate | / | 89.9% | 85.6% | 89.9% |
The average recovery rate of the mark-on sample of the different content of formaldehyde that as shown above, contrast experiment records is all less than surpassing 90%.
In this article, " equating ", " identical " etc. are not the restriction in strict mathematical meaning, it also can comprise it will be appreciated by those skilled in the art that and preparation or the error that allows while using this product and method.
Be to be understood that, although this instructions is described according to each embodiment, but not each embodiment only comprises an independently technical scheme, this narrating mode of instructions is only for clarity sake, those skilled in the art should make instructions as a whole, technical scheme in each embodiment also can, through appropriately combined, form other embodiments that it will be appreciated by those skilled in the art that.
Listed a series of detailed description is above only illustrating for feasibility embodiment of the present invention; they are not in order to limit the scope of the invention, all disengaging within equivalent embodiment that skill spirit of the present invention does or change all should be included in protection scope of the present invention.
Claims (5)
1. a method that detects content of formaldehyde in cow's milk, is characterized in that, comprising:
The standard solution of preparing different concentration of formaldehyde;
The acetonitrile solution of preparation DNPH, obtains DNPH solution;
In standard solution described in each, add respectively described DNPH solution and shake up, obtaining the standard reaction liquid corresponding to different concentration of formaldehyde;
Get cow's milk to be measured, add described DNPH solution and shake up, obtain sample reactant liquor;
The water-bath that standard reaction liquid described in each and described sample reactant liquor are put into 58 to 62 ℃ is reacted 9 to 11 minutes, in standard reaction liquid described in each and described sample reactant liquor, add normal hexane respectively after cooling, vortex centrifugal layering after 50 to 70 seconds, obtains standard centrifugate and sample centrifugate corresponding to different concentration of formaldehyde;
The supernatant liquor that filters standard centrifugate and described sample centrifugate described in each, obtains standard supernatant and sample supernatant corresponding to different concentration of formaldehyde, detects the gas chromatography value of standard supernatant and described sample supernatant described in each;
According to the concentration of formaldehyde in standard solution described in each and the gas chromatography value drafting corresponding with standard solution described in each, obtain typical curve;
According to gas chromatography value corresponding to described cow's milk to be measured and described typical curve, calculate the content of formaldehyde in described cow's milk to be measured.
2. a kind of method that detects content of formaldehyde in cow's milk as claimed in claim 1, the compound method of wherein said DNPH solution is: weigh 0.05g DNPH in small beaker, with acetonitrile, dissolve and be settled to 100mL.
3. a kind of method that detects content of formaldehyde in cow's milk as claimed in claim 1, wherein, processes every milliliter of described standard solution, and needing to use the amount of described DNPH solution is 0.5mL; Process every gram of described cow's milk to be measured, amount that need to use described DNPH solution is 0.4 to 0.6mL.
4. a kind of method that detects content of formaldehyde in cow's milk as claimed in claim 1, the condition of wherein said gas chromatographic detection is:
Chromatographic column is VF-5 capillary column, and specification is column length 30m, internal diameter 0.25mm, smearing thickness 0.25 μ m;
Injector temperature is 240 ℃;
Sample size is 1 μ L, Splitless injecting samples;
Post case temperature is 140 ℃ of initial temperature, with the speed of 20 ℃/min, is warming up to 280 ℃ and maintain 8min;
The temperature of electron capture detector is 300 ℃;
Carrier gas is nitrogen, and flow velocity is 1.0mL/min.
5. a kind of method that detects content of formaldehyde in cow's milk as claimed in claim 1, in wherein said standard solution, the concentration of formaldehyde is respectively: 0.0ng/mL, 25ng/mL, 50ng/mL, 100ng/mL and 200ng/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210255152.7A CN103575840A (en) | 2012-07-23 | 2012-07-23 | Method for detecting content of formaldehyde in milk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210255152.7A CN103575840A (en) | 2012-07-23 | 2012-07-23 | Method for detecting content of formaldehyde in milk |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103575840A true CN103575840A (en) | 2014-02-12 |
Family
ID=50048060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210255152.7A Pending CN103575840A (en) | 2012-07-23 | 2012-07-23 | Method for detecting content of formaldehyde in milk |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103575840A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104165950A (en) * | 2014-09-01 | 2014-11-26 | 云南中烟工业有限责任公司 | Detection method for formaldehyde in hot-melt adhesive |
| CN104198622A (en) * | 2014-09-26 | 2014-12-10 | 中华人民共和国东莞出入境检验检疫局 | Measuring method for formaldehyde content in melamine |
| CN105424844A (en) * | 2016-01-13 | 2016-03-23 | 山东联星能源集团有限公司 | Method for detecting formaldehyde in methanol gasoline fuel tail gas through capillary column gas chromatographic method |
| CN111257264A (en) * | 2020-03-18 | 2020-06-09 | 上海纽脉医疗科技有限公司 | Method for detecting aldehyde group content in biological tissue |
-
2012
- 2012-07-23 CN CN201210255152.7A patent/CN103575840A/en active Pending
Non-Patent Citations (4)
| Title |
|---|
| 张玉华等: "气相色谱法测定水中痕量甲醛", 《四川省卫生管理干部学院学报》 * |
| 王华等: "衍生气相色谱法测定啤酒中痕量甲醛", 《理化检验-化学分册》 * |
| 肖昌佐: "迪马科技发布牛奶和奶粉中甲醛的检测方案", 《中国色谱网》 * |
| 黄晓兰等: "气相色谱-质谱法测定食品中的甲醛", 《分析化学(FENXI HUAXUE)研究报告》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104165950A (en) * | 2014-09-01 | 2014-11-26 | 云南中烟工业有限责任公司 | Detection method for formaldehyde in hot-melt adhesive |
| CN104165950B (en) * | 2014-09-01 | 2016-02-03 | 云南中烟工业有限责任公司 | The detection method of formaldehyde in a kind of hot melt adhesive |
| CN104198622A (en) * | 2014-09-26 | 2014-12-10 | 中华人民共和国东莞出入境检验检疫局 | Measuring method for formaldehyde content in melamine |
| CN105424844A (en) * | 2016-01-13 | 2016-03-23 | 山东联星能源集团有限公司 | Method for detecting formaldehyde in methanol gasoline fuel tail gas through capillary column gas chromatographic method |
| CN111257264A (en) * | 2020-03-18 | 2020-06-09 | 上海纽脉医疗科技有限公司 | Method for detecting aldehyde group content in biological tissue |
| CN111257264B (en) * | 2020-03-18 | 2023-02-28 | 上海纽脉医疗科技有限公司 | Method for detecting aldehyde group content in biological tissue |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103575840A (en) | Method for detecting content of formaldehyde in milk | |
| CN101876651A (en) | Method for measuring main carbonyl compounds in main stream cigarette smoke | |
| CN101221154B (en) | Method for measuring myprozine content in milk and its products | |
| CN105699508B (en) | The analysis method of impurity content in PPG | |
| CN104111291B (en) | A kind of soil poisoning dead Ticks pesticide and the detection method of three kinds of metabolite thereof | |
| CN104198596B (en) | A kind of detection method of propylthiouracil sodium salt dissolvent residual | |
| CN101852767A (en) | Method for quickly detecting trace melamine | |
| CN104215725A (en) | Sample pretreatment method for detection of organic chlorine in milk and detection method using same | |
| CN112326847A (en) | Method for detecting impurities in isophthalonitrile | |
| CN102928521B (en) | The content assaying method of carbohydrate content in compound Salviae Miltiorrhizae extract | |
| CN104280474B (en) | In Simultaneously test essence spice for cigarette, 4 kinds limit the High Performance Liquid Chromatography with Fluorescence Detection with adjuvant | |
| CN103645151A (en) | Method used for rapid detection of spectinomycin content in spectinomycin broth or finished products | |
| CN106093268A (en) | A kind of detection method of Amitraz content | |
| CN103675137B (en) | Ornidazole injection liquid quality control method | |
| CN102393424A (en) | Analytic method for purity of N-cyclohexyl-2-benzothiazole sulfonamide as thiofide | |
| CN105467035A (en) | Method for detecting macromolecular substances in Shenxiong glucose injection | |
| CN105572240A (en) | Method for detecting content of pharmaceutic adjuvant carmine by using high performance liquid chromatography | |
| CN104569191A (en) | Detection method for ornithine in plasma | |
| CN104049046A (en) | Method for detecting dimethyl fumarate in body lotion | |
| CN104597188A (en) | Method for detecting residual quantity of Dieldrin in dairy products | |
| CN106872629A (en) | A kind of method of three nitrogen amidine contents in measure dairy products | |
| CN101025407A (en) | Analytical method for determining micro moisture in cyclopropyl amine by gas phase chromatography | |
| CN107677737A (en) | A kind of method that chloroform is remained in detection Tretinoin | |
| CN104316636B (en) | Utilize the internal standard assay method of benzene and benzene homologues in the glyceryl triacetate of head space-gaseous mass spectrum | |
| CN106290622A (en) | The detection method of Radix Et Rhizoma Rhei aldehyde in a kind of diacerein raw material finished product |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140212 |