CN103571911A - Selective medium for staphylococcus chromogenes - Google Patents
Selective medium for staphylococcus chromogenes Download PDFInfo
- Publication number
- CN103571911A CN103571911A CN201310564866.0A CN201310564866A CN103571911A CN 103571911 A CN103571911 A CN 103571911A CN 201310564866 A CN201310564866 A CN 201310564866A CN 103571911 A CN103571911 A CN 103571911A
- Authority
- CN
- China
- Prior art keywords
- selective medium
- agar
- staphylococcus
- lily
- coetsoidin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000006152 selective media Substances 0.000 title claims abstract description 33
- 241000201854 Staphylococcus chromogenes Species 0.000 title abstract description 4
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 229920001817 Agar Polymers 0.000 claims abstract description 21
- 239000008272 agar Substances 0.000 claims abstract description 21
- 241000234435 Lilium Species 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims abstract description 16
- 241000168720 Panax japonicus Species 0.000 claims abstract description 15
- 235000003174 Panax japonicus Nutrition 0.000 claims abstract description 15
- UOYUDLWZKQVTAO-UTQDMZSBSA-N [(1S,2S,5S,7R,8S)-10-hydroxy-12,12-dimethyl-6-methylidene-9,16-dioxo-14-oxapentacyclo[11.2.2.15,8.01,11.02,8]octadec-10-en-7-yl] acetate Chemical compound CC(=O)O[C@@H]1C(=C)[C@@H]2C[C@]11[C@@H](CC2)[C@]23COC(CC2=O)C(C)(C)C3=C(O)C1=O UOYUDLWZKQVTAO-UTQDMZSBSA-N 0.000 claims abstract description 15
- FCIGPGKXILHDAK-UHFFFAOYSA-N coetsoidin A Natural products CC1(C)C2CC(O)C34C(O)C(CCC3C25CCC1(O)OC5)C(=C)C4=O FCIGPGKXILHDAK-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910000365 copper sulfate Inorganic materials 0.000 claims abstract description 10
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 claims description 28
- 241000191940 Staphylococcus Species 0.000 claims description 26
- 101000693916 Gallus gallus Albumin Proteins 0.000 claims description 17
- 238000002360 preparation method Methods 0.000 claims description 15
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims description 14
- 229910019142 PO4 Inorganic materials 0.000 claims description 14
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 14
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 14
- 239000000395 magnesium oxide Substances 0.000 claims description 14
- IUCHKMAZAWJNBJ-RCYXVVTDSA-N oleanolic acid 3-O-beta-D-glucosiduronic acid Chemical compound O([C@H]1CC[C@]2(C)[C@H]3CC=C4[C@@]([C@@]3(CC[C@H]2C1(C)C)C)(C)CC[C@]1(CCC(C[C@H]14)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O IUCHKMAZAWJNBJ-RCYXVVTDSA-N 0.000 claims description 14
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 14
- 239000010452 phosphate Substances 0.000 claims description 14
- 229910052700 potassium Inorganic materials 0.000 claims description 14
- 239000011591 potassium Substances 0.000 claims description 14
- 238000010411 cooking Methods 0.000 claims description 13
- 238000000926 separation method Methods 0.000 abstract description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 abstract 1
- 102000002322 Egg Proteins Human genes 0.000 abstract 1
- 108010000912 Egg Proteins Proteins 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 239000012530 fluid Substances 0.000 abstract 1
- 229960005336 magnesium citrate Drugs 0.000 abstract 1
- 239000004337 magnesium citrate Substances 0.000 abstract 1
- 235000002538 magnesium citrate Nutrition 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 1
- 229930182490 saponin Natural products 0.000 abstract 1
- 150000007949 saponins Chemical class 0.000 abstract 1
- 235000017709 saponins Nutrition 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- PLSARIKBYIPYPF-UHFFFAOYSA-H trimagnesium dicitrate Chemical compound [Mg+2].[Mg+2].[Mg+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O PLSARIKBYIPYPF-UHFFFAOYSA-H 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 description 15
- 230000001954 sterilising effect Effects 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 11
- 238000001914 filtration Methods 0.000 description 6
- 238000012856 packing Methods 0.000 description 6
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 229910001385 heavy metal Inorganic materials 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical class OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000012913 prioritisation Methods 0.000 description 3
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- LRADVKQALJANQG-UHFFFAOYSA-N 2-chlorophenol;sodium Chemical compound [Na].OC1=CC=CC=C1Cl LRADVKQALJANQG-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 240000008058 Lilium brownii Species 0.000 description 1
- 244000210789 Lilium lancifolium Species 0.000 description 1
- 241001634090 Lilium pumilum Species 0.000 description 1
- 241001359444 Lilium tenuifolium Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241001474728 Satyrodes eurydice Species 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 241000295644 Staphylococcaceae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 1
- 235000013734 beta-carotene Nutrition 0.000 description 1
- 239000011648 beta-carotene Substances 0.000 description 1
- 229960002747 betacarotene Drugs 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 101150073818 gap gene Proteins 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a selective medium for staphylococcus chromogenes. The selective medium is characterized in that a formula for preparing 1000 ml of the selective medium contains 15 g of malt extract powder, 150 ml of egg protein fluid, 5 g of sodium chloride, 0.5 g of magnesium citrate, 15 g of agar, 8-12 mg of coetsoidin A, 0.6 g of panax japonicus saponins Ib, 0.2 g of monopotassium phosphate, 5 mg of copper sulfate, 0.1 g of L-cysteine, and 200-300 ml of lily boiled liquid with a concentration of 200 g/l. Compared with the prior art, the selective medium disclosed by the invention has the characteristics of reliable detection, and high separation rate of staphylococcus chromogenes.
Description
Technical field
The present invention relates to field of medical examination, the division of the patent of invention < < Staphylococcus chomogenes selective medium that the application number that is application on January 13rd, 2013 is 2013100107409 and preparation method thereof > >, is specifically related to a kind of Staphylococcus chomogenes selective medium.
Background technology
Staphylococcus chomogenes (Staphylococcus chromogenes) is the rare pathogenic bacterias of the mankind, belong to low virulence conditioned pathogen, atypical symptom after infecting, and present multidrug resistant phenomenon, bring certain difficulty to clinical diagnosis and treatment, the method for inspection of high efficient and reliable has been to need.
The most reliable authentication method of Staphylococcus chomogenes, for utilizing the amplification of gap gene pairs sample and order-checking, obtains sequence and compares at GenBank at present, simultaneously with 16S rRNA genetic comparison, and then determines its somatotype.But in current check and study of pharmacy, still lack for the special microbiological culture media of Staphylococcus chomogenes.So-called microorganism culturing is a kind of technology that makes by artificial means microorganism growth breeding, and selectivity selective medium is for promoting or suppressing the organism (as cell or bacterium etc.) of certain type and the selective medium of design utilizes this selective medium needed microorganism can be separated from the microorganism mixing.
The staphylococcus selective medium generally using clinically is at present manitol salt agar (Manitol Salt Agar), for the selective separation of streptococcus aureus, cultivates.Medium component is: peptone, Chicken Albumin liquid, PEARLITOL 25C, sodium-chlor, phenol red, agar.Streptococcus aureus bacterium colony is yellow, and intestinal bacteria and Salmonellas bacterium colony are colourless.But this kind of substratum can not selectivity be cultivated Staphylococcus chomogenes (CNS), its reason is that streptococcus aureus is faster than Staphylococcus chomogenes growth in current selective medium, has covered very soon substratum, and CNS cannot grow.
Summary of the invention
Technical assignment of the present invention is for above the deficiencies in the prior art, and a kind of selective medium high to Staphylococcus chomogenes separation rate is provided.
The technical scheme that the present invention solves its technical problem is: a kind of Staphylococcus chomogenes selective medium, and its feature exists, and in the formula of preparation 1000ml selective medium, contains:
Fructus Hordei Germinatus soaks powder 15g;
Chicken Albumin liquid 150ml;
Sodium-chlor 5g;
Citrate of magnesia 0.5g;
Agar 15g;
Coetsoidin A 8 ~ 12mg;
Panax japonicus saponin I b 0.6 g;
Potassium primary phosphate 0.2g;
Distilled water adds to 1000ml.
In prioritization scheme, every 1000ml selective medium also contains copper sulfate 5mg.
In prioritization scheme, every 1000ml selective medium also contains Cys 0.1g.
In prioritization scheme, every 1000ml selective medium also contains lily water cooking liquid 200 ~ 300ml of concentration 200g/L.
In optimization scheme, every 1000ml selective medium also contains copper sulfate 5mg; Cys 0.1g; Lily water cooking liquid 200 ~ 300ml of concentration 200g/L.
A preparation method for Staphylococcus chomogenes selective medium, is characterized in that comprising the following steps:
(1) get lily and add water boil 1 hour, filter, get filtrate, adjusting volume is 200g/L to concentration, obtains lily water cooking liquid;
(2) get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; Panax japonicus saponin I b; Potassium primary phosphate; Cys and copper sulfate are dissolved in step 1 gained lily water cooking liquid, mix after filtration distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after sterilizing.
Wherein said Fructus Hordei Germinatus soaks powder, Chicken Albumin liquid provides carbon nitrogen source and energy; Citrate of magnesia and potassium primary phosphate are buffer reagent, and can suppress varied bacteria growing; Cys belongs to amino acids, is nutrition agent; Agar is the peptizer of selective medium; Coetsoidin A has special bacteriostatic action for golden Portugal bacterium, invalid for Staphylococcus chomogenes; Chicken Albumin liquid contains each peptide species, fat, somatomedin, hormone etc., and these materials are to promoting that the Growth and Reproduction of Staphylococcus chomogenes is all very important factor, and Chicken Albumin liquid also has the constant effect of selective medium pH value that maintains in addition.Panax japonicus saponin I b is Staphylococcus chomogenes specificity nutrition agent, can specific promotion Staphylococcus chomogenes breeding.Cupric ion belongs to heavy metal ion, under this concentration dose of 5mg/L, be deposited on golden Portugal bacterium inner, cause cell wall to disappear or attenuation, rrna is gathered into agglomerate, finally causes bacterium distortion and breaks, present the property damage of heavy metal source, and Staphylococcus chomogenes to the transportcapacity of heavy metal and penetrating power lower than golden Portugal bacterium, be also that the resistance of heavy metal is higher, so add copper sulfate can effectively suppress golden Portugal bacteria growing.Lily is the dry meat scale leaf of liliaceous plant tiger lily Lilium lancifolium Thunb., lily Lilium brownii F.E.Brown var. viridulum Baker or Lilium tenuifolium Lilium pumilum DC., contain the Multiple components such as protein, fat, starch, also first sugar, VITMAIN B1, B2, pantothenic acid, vitamins C, β-carotene, energy metabolism, cell development, the metabolic factor can be provided, help lend some impetus to Staphylococcus chomogenes growth, the especially growth of bacterium colony under heavy metal environment.
The present invention compared with prior art, has the feature reliable, Staphylococcus chomogenes separation rate is high that detects.
Embodiment
Below in conjunction with practical situation, the specific embodiment of the present invention is elaborated.
Embodiment 1, contains: Fructus Hordei Germinatus soaks powder 15g in the formula of preparation 1000ml selective medium; Chicken Albumin liquid 150ml; Sodium-chlor 5g; Citrate of magnesia 0.5g; Agar 15g; Coetsoidin A 8mg; Panax japonicus saponin I b 0.6 g; Potassium primary phosphate
0.2g; Distilled water adds to 1000ml.Preparation method: get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; Panax japonicus saponin I b; Potassium primary phosphate is dissolved in distilled water, mixes after filtration distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after sterilizing.
Embodiment 2, contain: Fructus Hordei Germinatus soaks powder 15g in the formula of preparation 1000ml selective medium; Chicken Albumin liquid 150ml; Sodium-chlor 5g; Citrate of magnesia 0.5g; Agar 15g; Coetsoidin A 12mg; Panax japonicus saponin I b 0.6 g; Potassium primary phosphate
0.2g; Copper sulfate 5mg; Distilled water adds to 1000ml.Preparation method: get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; Panax japonicus saponin I b; Potassium primary phosphate; Copper sulfate is dissolved in distilled water, mixes after filtration distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after sterilizing.
Embodiment 3, contain: Fructus Hordei Germinatus soaks powder 15g in the formula of preparation 1000ml selective medium; Chicken Albumin liquid 150ml; Sodium-chlor 5g; Citrate of magnesia 0.5g; Agar 15g; Coetsoidin A 10mg; Panax japonicus saponin I b 0.6 g; Potassium primary phosphate
0.2g; Cys
0.1g; Distilled water adds to 1000ml.Preparation method: get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; Panax japonicus saponin I b; Potassium primary phosphate; Cys is dissolved in distilled water, mixes after filtration distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after sterilizing.
Embodiment 4, contain: Fructus Hordei Germinatus soaks powder 15g in the formula of preparation 1000ml selective medium; Chicken Albumin liquid 150ml; Sodium-chlor 5g; Citrate of magnesia 0.5g; Agar 15g; Coetsoidin A 9mg; Panax japonicus saponin I b 0.6 g; Potassium primary phosphate
0.2g; The lily water cooking liquid 200ml of concentration 200g/L; Distilled water adds to 1000ml.Preparation method:
(1) get lily and add water boil 1 hour, filter, get filtrate, adjusting volume is 200g/L to concentration, obtains lily water cooking liquid;
(2) get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; Panax japonicus saponin I b; Potassium primary phosphate; Be dissolved in step 1 gained lily water cooking liquid, mix after filtration, distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after sterilizing.
Embodiment 5, contain: Fructus Hordei Germinatus soaks powder 15g in the formula of preparation 1000ml selective medium; Chicken Albumin liquid 150ml; Sodium-chlor 5g; Citrate of magnesia 0.5g; Agar 15g; Coetsoidin A 11mg; Panax japonicus saponin I b 0.6 g; Potassium primary phosphate
0.2g; Copper sulfate 5mg; Cys
0.1g; The lily water cooking liquid 300ml of concentration 200g/L; Distilled water adds to 1000ml.Preparation method is:
(1) get lily and add water boil 1 hour, filter, get filtrate, adjusting volume is 200g/L to concentration, obtains lily water cooking liquid;
(2) get Fructus Hordei Germinatus and soak powder; Chicken Albumin liquid; Sodium-chlor; Citrate of magnesia; Agar; Coetsoidin A; Panax japonicus saponin I b; Potassium primary phosphate; Cys and copper sulfate are dissolved in step 1 gained lily water cooking liquid, mix after filtration distilled water constant volume, temperature sterilization 2 times, each 45 min, packing after sterilizing.
Described lily water cooking liquid concentration is 200g/L, refers in every liter of lily water cooking liquid and contains crude drug 200g.
Gained Staphylococcus chomogenes selective medium of the present invention has and detects the feature reliable, Staphylococcus chomogenes separation rate is high, and for clinical data fully proves, pertinent data is as follows.
1 object and method.
1.1 selective medium preparations: establish altogether 3 groups, be respectively control group, embodiment 1 scheme group, embodiment 5 scheme groups.Control group is staphylococcus selective agar 110(CHAPMAN agar), filling a prescription is: pancreas casein peptone, yeast soak powder, gelatin, PEARLITOL 25C, lactose, sodium-chlor, dipotassium hydrogen phosphate, agar.Formula and the preparation method of embodiment 1 scheme group, embodiment 5 scheme groups are shown in embodiment.
1.2 methods: by sneaking into gold-coloured staphylococci in 20 parts of samples of determining the Staphylococcus chomogenes positive via PCR, be inoculated on substratum, hatch at 37 ℃ 24 hours.
1.3 statistical analysis: carry out statistical study with SPSS 13.0, P< 0.05 indicates significant.
2 results: in 20 parts of inoculation samples, microbial culture is separated and be accredited as Staphylococcus chomogenes, and embodiment 1 scheme group is 16 examples, and embodiment 5 scheme groups are 18 examples.Control group CHAPMAN nutrient agar bacterium colony is golden Portugal bacterium through identifying to be finally proved, and learns and processes by statistics, and various embodiments of the present invention group and control group relatively have notable difference (P<0.001).Result shows, embodiment of the present invention Staphylococcus chomogenes separation rate is apparently higher than existing selective medium.
Claims (1)
1. a Staphylococcus chomogenes selective medium, its feature exists, and contains: Fructus Hordei Germinatus soaks powder 15g in the formula of preparation 1000ml selective medium; Chicken Albumin liquid 150ml; Sodium-chlor 5g; Citrate of magnesia 0.5g; Agar 15g; Coetsoidin A 8 ~ 12mg; Panax japonicus saponin I b 0.6 g; Potassium primary phosphate 0.2g; Copper sulfate 5mg; Cys 0.1g; Lily water cooking liquid 200 ~ 300ml of concentration 200g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310564866.0A CN103571911B (en) | 2013-01-13 | 2013-01-13 | A kind of Staphylococcus chomogenes selective medium |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310564866.0A CN103571911B (en) | 2013-01-13 | 2013-01-13 | A kind of Staphylococcus chomogenes selective medium |
| CN201310010740.9A CN103014125B (en) | 2013-01-13 | 2013-01-13 | Staphylococcus chromogenes selective culture medium and preparation method thereof |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310010740.9A Division CN103014125B (en) | 2013-01-13 | 2013-01-13 | Staphylococcus chromogenes selective culture medium and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103571911A true CN103571911A (en) | 2014-02-12 |
| CN103571911B CN103571911B (en) | 2015-09-30 |
Family
ID=50044620
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310564866.0A Expired - Fee Related CN103571911B (en) | 2013-01-13 | 2013-01-13 | A kind of Staphylococcus chomogenes selective medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103571911B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090083505A (en) * | 2008-01-30 | 2009-08-04 | 주식회사 바이오랜드 | A method for preparing fermented ginseng of ginseng extract using lactobacillus fermentum bljh101 |
| CN101874118A (en) * | 2007-11-26 | 2010-10-27 | 生物梅里埃公司 | Detect and/or identify the reaction culture medium of streptococcus aureus |
| CN102703592A (en) * | 2012-06-09 | 2012-10-03 | 江苏师范大学 | Methods for acquiring gap gene sequence of Staphylococcus chromogenes and primers of gap gene sequence |
| CN103014125B (en) * | 2013-01-13 | 2014-01-15 | 威海市妇女儿童医院 | Staphylococcus chromogenes selective culture medium and preparation method thereof |
-
2013
- 2013-01-13 CN CN201310564866.0A patent/CN103571911B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101874118A (en) * | 2007-11-26 | 2010-10-27 | 生物梅里埃公司 | Detect and/or identify the reaction culture medium of streptococcus aureus |
| KR20090083505A (en) * | 2008-01-30 | 2009-08-04 | 주식회사 바이오랜드 | A method for preparing fermented ginseng of ginseng extract using lactobacillus fermentum bljh101 |
| CN102703592A (en) * | 2012-06-09 | 2012-10-03 | 江苏师范大学 | Methods for acquiring gap gene sequence of Staphylococcus chromogenes and primers of gap gene sequence |
| CN103014125B (en) * | 2013-01-13 | 2014-01-15 | 威海市妇女儿童医院 | Staphylococcus chromogenes selective culture medium and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 傅冬梅等: "产色葡萄球菌临床分离株耐药性及耐药机制分析", 《国际检验医学杂志》 * |
| 杨有武: "青海地区奶牛乳房炎葡萄球菌的分离鉴定与药敏试验", 《中国畜牧兽医》 * |
| 王雪芬等: "假细锥香茶菜新二萜成分—假细锥甲素的结构", 《药学学报》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103571911B (en) | 2015-09-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101338283B (en) | Lactobacillus casei and applications thereof in solid-state fermentation | |
| CN103421715B (en) | Lactobacillus rhamnosus and application thereof | |
| CN105062933A (en) | Lactobacillus reuteri and application thereof | |
| CN110628663B (en) | Lactobacillus rhamnosus and high-density culture method and application thereof | |
| CN116042469A (en) | Lactobacillus metazoan compound with antibacterial function and preparation method and application thereof | |
| CN114015661A (en) | Culture medium and method for improving titer of phage | |
| CN105039202A (en) | Selective medium for multiplex proliferation of salmonella, listeria monocytogenes and vibrio parahaemolyticus and preparation method of selective medium | |
| CN109182397B (en) | Method for improving yield of reuterin | |
| CN108410763B (en) | Bifidobacterium longum TC01 and application and product using same | |
| CN104560835A (en) | Culture medium for culturing mycoplasma hyopneumoniae and preparation method thereof | |
| CN103409495B (en) | Preparation method of saprophytic staphylococcus selective medium | |
| CN103014125B (en) | Staphylococcus chromogenes selective culture medium and preparation method thereof | |
| CN104046584A (en) | Bifidobacterium adolescentis bacteriocin as well as production method and special production strain of bifidobacterium adolescentis bacteriocin | |
| CN105624071A (en) | Lactobacillus salivarius XJP2 and application thereof | |
| CN104212745B (en) | Chicken microecological preparation and preparation method thereof | |
| CN114540214B (en) | Microorganism for high-yield organic chromium and application thereof | |
| CN103571911B (en) | A kind of Staphylococcus chomogenes selective medium | |
| CN103014123B (en) | Staphylococcus saprophyticus selective culture medium and preparation method thereof | |
| CN115960760A (en) | Pediococcus pentosaceus with cholesterol reducing and bacteriostatic effects and high-density industrial production fermentation medium thereof | |
| CN103014127B (en) | Staphylococcus lentus selective medium and preparation method thereof | |
| CN103014128A (en) | Staphylococcus cohnii selective medium and preparation method thereof | |
| CN103014130B (en) | Staphylococcus xylosus selective culture medium and preparation method thereof | |
| CN109161501B (en) | Feeding bacillus licheniformis and application thereof | |
| CN103497988B (en) | Fermentation production method of safe efficient lactobacillus product | |
| CN108102960B (en) | Lactobacillus acidophilus NCFM acid-resistant protective agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: QI HONGMIN Free format text: FORMER OWNER: LIU MINGXIA Effective date: 20150820 Owner name: SONG GUANGZHOU XIAO JING Effective date: 20150820 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Qi Hongmin Inventor after: Song Guangzhou Inventor after: Xiao Jing Inventor after: Qu Xuliang Inventor after: Sun Lili Inventor before: Liu Mingxia |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: LIU MINGXIA TO: QI HONGMIN SONG GUANGZHOU XIAO JING QU XULIANG SUN LILI |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20150820 Address after: 261400 Laizhou maternal and child health care hospital, 288 east culture street, Yantai, Shandong, Laizhou Applicant after: Qi Hongmin Applicant after: Song Guangzhou Applicant after: Xiao Jing Address before: 276826 Rizhao City traditional Chinese medicine hospital, 35 Hai Lu, Shandong, Rizhao City Applicant before: Liu Mingxia |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150930 Termination date: 20160113 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |