CN103571880A - Method for producing fuel ethanol by beets - Google Patents
Method for producing fuel ethanol by beets Download PDFInfo
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- CN103571880A CN103571880A CN201210267448.0A CN201210267448A CN103571880A CN 103571880 A CN103571880 A CN 103571880A CN 201210267448 A CN201210267448 A CN 201210267448A CN 103571880 A CN103571880 A CN 103571880A
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- yeast cell
- fermentation
- yeast
- alcohol
- gel particle
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
A method for producing fuel ethanol by beets comprises the following steps: 1. preparing corn pulp; 2. preparing an immobilized yeast cell activation proliferation medium; 3. immobilizing yeast cells; 4. proliferating the immobilized yeast cells; 5. performing alcohol continuous fermentation under the controlled condition that the sugar degree of the fermented liquid is 10%-20%, the adding amount of ammonium sulfate is 0.5-1.0 g/L, the pH of the fermentation nutrient solution is 4.0-4.5, the loading amount of the immobilized yeast cell gel particles is 45-55%, the fermentation temperature is 30 DEG C, the sweet mash feeding speed is 15-16 mL/h, the dwell time is 3-4 hours, and the dilution rate is 0.15-0.16 h<-1>; 6. determining the stability of the polyvinyl alcohol (PVA) gel particles. The beneficial effect of the present invention is that: through yeast immobilization, the method of the invention realizes yeast recycle during fuel alcohol production by beet fermentation, and improves the fermentation efficiency and yeast stability.
Description
Technical field
The present invention relates to energy field, specifically a kind of method of utilizing beet to produce alcohol fuel.
Background technology
Fuel alcohol, as a kind of reproducible clear energy sources, can substitute day by day rare, non-renewable petrochemicals, has wide market outlook.Yet tradition has limited promoting the use of of fuel alcohol with the huge cost of grain-production alcohol, and take the grains such as corn as raw material production alcohol, must cause higher food prices.Therefore, seek the focus that alcohol biomass material cheap and easy to get becomes domestic and international research.
(Beta vulgaris) You Ming Chard dish belongs to Chenopodiaceae (Familia Chenopodiaceae) Beta (Genus Beta) to beet.Originating in European western part and Southern Coast, is one of main sugar crop of the main sugared Yuan，Ye Shi of except sugarcane one China.The main chemical composition analysis of beet root shows, total reducing sugar 17.3%, moisture 75%, Mierocrystalline cellulose, hemicellulose 3.3%, pectin substance 2.3%, protein and other nitrogenous substancess 1.5%, ash content 0.6%.Due to Sugar―beet biology output and sugar degree higher, be also one of fuel ethanol production raw material that the current world is emerging, tool grows a lot potentiality.
Summary of the invention
The object of this invention is to provide a kind of method of utilizing beet to produce alcohol fuel.
Technical scheme of the present invention is: a kind of method of utilizing beet to produce alcohol fuel, it includes
1, prepare corn slurries: in 1L water, add 30g Semen Maydis powder, be heated to be (90 ± 2) ℃, add α-amylaseliquefied 35min, cool to (55 ± 2) ℃, add saccharifying enzyme saccharification 50min.
2, prepare fixed yeast cell activation and proliferation substratum: glucose 20g, corn slurries 30mL, ammonium sulfate 0.5g, potassium primary phosphate 0.02g, ferrous sulfate 0.003g, adds water to lO00mL, cooling standby after conventional autoclaving.
3, the immobilization of yeast cell: after appropriate active dry yeast is placed in to 30 ℃ of sterilized waters and melts, mix with 6% polyvinyl alcohol water solution that contains 0.6% sodium alginate in proportion, with syringe, dropwise splash into containing in 5% borate of 1% calcium chloride again, making diameter is the immobilization gel particle of 3mm left and right, solidify 50min, leach immobilization gel particle, with physiological saline washing 3 times, immerse in sterilized water standby.
4, the propagation of fixed yeast cell: fixed yeast cell gel particle is embathed 5 times with sterilized water, add the fixed yeast activation and proliferation substratum of same volume, in 30 ℃, the 120r/min shaking culture of ventilating, after 13h, yeast cell starts propagation.When incubation time is during to 24h, free yeast number is 3.8x10
7individual/more than mL, to complete breeding.
5, immobilized biocatalyst alcohol continuous fermentation: the substratum that continuously ferments of getting ready is inputted in immobilization bioreactor by peristaltic pump constant speed, 30 ℃ of controlled fermentation temperature are 15-16mL/h, the residence time to be 3-4h and dilution rate 0.15-0.16h in converted mash stream rate of acceleration
-1under condition, carry out alcohol continuous fermentation.
6, determine the stability of polyvinyl alcohol (PVA) gel particle.
Preferably: described fermented liquid pol is 10%-20%.
Preferably: described ammonium sulfate addition is 0.5-1.0g/L.
Preferably: described fermentation culture pH is 4.0-4.5.
Preferably: described fixed yeast cell gel particle amount of fill is 45-55%.
The invention has the beneficial effects as follows: the present invention, by by yeast immobilization, can reuse yeast, and improved the stability of fermentation efficiency and yeast when fermentation beet is produced fuel alcohol.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1
Utilize beet to produce a method for alcohol fuel, it includes
1, prepare corn slurries: in 1L water, add 30g Semen Maydis powder, be heated to be (90 ± 2) ℃, add α-amylaseliquefied 35min, cool to (55 ± 2) ℃, add saccharifying enzyme saccharification 50min.
2, prepare fixed yeast cell activation and proliferation substratum: glucose 20g, corn slurries 30mL, ammonium sulfate 0.5g, potassium primary phosphate 0.02g, ferrous sulfate 0.003g, adds water to lO00mL, cooling standby after conventional autoclaving.
3, the immobilization of yeast cell: after appropriate active dry yeast is placed in to 30 ℃ of sterilized waters and melts, mix with 6% polyvinyl alcohol water solution that contains 0.6% sodium alginate in proportion, with syringe, dropwise splash into containing in 5% borate of 1% calcium chloride again, making diameter is the immobilization gel particle of 3mm left and right, solidify 50min, leach immobilization gel particle, with physiological saline washing 3 times, immerse in sterilized water standby.
4, the propagation of fixed yeast cell: fixed yeast cell gel particle is embathed 5 times with sterilized water, add the fixed yeast activation and proliferation substratum of same volume, in 30 ℃, the 120r/min shaking culture of ventilating, after 13h, yeast cell starts propagation.When incubation time is during to 24h, free yeast number is 3.8x10
7individual/more than mL, to complete breeding.
5, immobilized biocatalyst alcohol continuous fermentation: the substratum that continuously ferments of getting ready is inputted in immobilization bioreactor by peristaltic pump constant speed, fermented liquid pol is 10%, ammonium sulfate addition is 0.6g/L, fermentation culture pH is 4.0, fixed yeast cell gel particle amount of fill is 45%, 30 ℃ of controlled fermentation temperature are 15-16mL/h, the residence time to be 3-4h and dilution rate 0.15-0.16h in converted mash stream rate of acceleration
-1under condition, carry out alcohol continuous fermentation.Now, total reducing sugar utilization ratio is 68.78%.
6, determine the stability of polyvinyl alcohol (PVA) gel particle, find that immobilization gel particle finds no disrepair phenomenon.
Embodiment 2
Other operations and condition are constant, changing the condition of continuously fermenting is: fermented liquid pol is 15%, ammonium sulfate addition is 0.8g/L, fermentation culture pH is 4.3, fixed yeast cell gel particle amount of fill is 48%, 30 ℃ of controlled fermentation temperature are 15-16mL/h, the residence time to be 3-4h and dilution rate 0.15-0.16h in converted mash stream rate of acceleration
-1under condition, carry out alcohol continuous fermentation.Now, total reducing sugar utilization ratio is 78.15%.Do not find that immobilization gel particle do not find disrepair phenomenon.
Embodiment 3
Other operations and condition are constant, changing the condition of continuously fermenting is: fermented liquid pol is 18%, ammonium sulfate addition is 1.0g/L, fermentation culture pH is 4.5, fixed yeast cell gel particle amount of fill is 55%, 30 ℃ of controlled fermentation temperature are 15-16mL/h, the residence time to be 3-4h and dilution rate 0.15-0.16h in converted mash stream rate of acceleration
-1under condition, carry out alcohol continuous fermentation.Now, total reducing sugar utilization ratio is 89.36%.Do not find that immobilization gel particle do not find disrepair phenomenon.
Claims (6)
1. utilize beet to produce a method for alcohol fuel, it includes
A, prepare corn slurries: in 1L water, add 30g Semen Maydis powder, be heated to be (90 ± 2) ℃, add α-amylaseliquefied 35min, cool to (55 ± 2) ℃, add saccharifying enzyme saccharification 50min;
B, B, prepare fixed yeast cell activation and proliferation substratum: glucose 20g, corn slurries 30mL, ammonium sulfate 0.5g, potassium primary phosphate 0.02g, ferrous sulfate 0.003g, adds water to lO00mL, cooling standby after conventional autoclaving;
The immobilization of C, yeast cell: after appropriate active dry yeast is placed in to 30 ℃ of sterilized waters and melts, mix with 6% polyvinyl alcohol water solution that contains 0.6% sodium alginate in proportion, with syringe, dropwise splash into containing in 5% borate of 1% calcium chloride again, making diameter is the immobilization gel particle of 3mm left and right, solidify 50min, leach immobilization gel particle, with physiological saline washing 3 times, immerse in sterilized water standby;
The propagation of D, fixed yeast cell: fixed yeast cell gel particle is embathed 5 times with sterilized water, add the fixed yeast activation and proliferation substratum of same volume, in 30 ℃, the 120r/min shaking culture of ventilating, after 13h, yeast cell starts propagation.
2. when incubation time is during to 24h, free yeast number is 3.8x10
7individual/more than mL, to complete breeding;
E, immobilized biocatalyst alcohol continuous fermentation: the substratum that continuously ferments of getting ready is inputted in immobilization bioreactor by peristaltic pump constant speed, 30 ℃ of controlled fermentation temperature are 15-16mL/h, the residence time to be 3-4h and dilution rate 0.15-0.16h in converted mash stream rate of acceleration
-1under condition, carry out alcohol continuous fermentation;
F, determine the stability of polyvinyl alcohol (PVA) gel particle.
3. according to a kind of method of utilizing beet to produce alcohol fuel claimed in claim 1, it is characterized in that: described fermented liquid pol is 10%-20%.
4. according to a kind of method of utilizing beet to produce alcohol fuel claimed in claim 1, it is characterized in that: described ammonium sulfate addition is 0.5-1.0g/L.
5. according to a kind of method of utilizing beet to produce alcohol fuel claimed in claim 1, it is characterized in that: described fermentation culture pH is 4.0-4.5.
6. according to a kind of method of utilizing beet to produce alcohol fuel claimed in claim 1, it is characterized in that: described fixed yeast cell gel particle amount of fill is 45-55%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210267448.0A CN103571880A (en) | 2012-07-31 | 2012-07-31 | Method for producing fuel ethanol by beets |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210267448.0A CN103571880A (en) | 2012-07-31 | 2012-07-31 | Method for producing fuel ethanol by beets |
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| CN103571880A true CN103571880A (en) | 2014-02-12 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105238698A (en) * | 2015-08-17 | 2016-01-13 | 南京师范大学 | Method for transferring beet powder into enzyme yeast and health care products by using eurotium |
| CN105420286A (en) * | 2015-11-24 | 2016-03-23 | 广西罗城科潮基业科技发展有限公司 | Alcohol production method |
| CN108690666A (en) * | 2017-04-06 | 2018-10-23 | 阿尔卑斯技术株式会社 | A kind of preparation method of fuel additive, fuel additive and application thereof |
| CN109486873A (en) * | 2018-07-13 | 2019-03-19 | 滨州职业学院 | A kind of immobilization Combined culture fermentation Unicell Oils and Fats technology |
| CN113106029A (en) * | 2021-04-20 | 2021-07-13 | 南京工业大学 | Saccharomyces cerevisiae engineering bacterium for over-expressing ARO8 gene and construction method and application thereof |
-
2012
- 2012-07-31 CN CN201210267448.0A patent/CN103571880A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105238698A (en) * | 2015-08-17 | 2016-01-13 | 南京师范大学 | Method for transferring beet powder into enzyme yeast and health care products by using eurotium |
| CN105420286A (en) * | 2015-11-24 | 2016-03-23 | 广西罗城科潮基业科技发展有限公司 | Alcohol production method |
| CN108690666A (en) * | 2017-04-06 | 2018-10-23 | 阿尔卑斯技术株式会社 | A kind of preparation method of fuel additive, fuel additive and application thereof |
| CN109486873A (en) * | 2018-07-13 | 2019-03-19 | 滨州职业学院 | A kind of immobilization Combined culture fermentation Unicell Oils and Fats technology |
| CN113106029A (en) * | 2021-04-20 | 2021-07-13 | 南京工业大学 | Saccharomyces cerevisiae engineering bacterium for over-expressing ARO8 gene and construction method and application thereof |
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Application publication date: 20140212 |