CN103570777A - Extraction and purification method of stevioside RA - Google Patents
Extraction and purification method of stevioside RA Download PDFInfo
- Publication number
- CN103570777A CN103570777A CN201310531197.7A CN201310531197A CN103570777A CN 103570777 A CN103570777 A CN 103570777A CN 201310531197 A CN201310531197 A CN 201310531197A CN 103570777 A CN103570777 A CN 103570777A
- Authority
- CN
- China
- Prior art keywords
- steviosides
- purification
- extraction process
- ethanol
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Seasonings (AREA)
Abstract
The invention relates to an extraction and purification method of stevioside RA. The method comprises the following steps: (1) extraction: extracting stevioside RA in a raw material stevia rebaudiana dried leaves to obtain a percolate by adopting a percolation method; (2) flocculation: adjusting the pH to 10-11 by lime, adding a flocculant, flocculating, standing and settling and filter pressing; (3) chromatography: passing the filtrate through a chromatographic column filled with macroporous resin, washing a column bed by deionized water till the effluent is colorless, and then eluting by ethanol till the effluent is colorless; (4) desalination: passing the ethanol eluant through a chromatographic column filled with ion exchange resin, and collecting a column effluent; (5) decoloration: adding active carbon, heating to reflux and filtering while heating; (6) concentration: carrying out decompressed concentration on the filtrate to obtain a crude product; and (7) purification: adding methanol into the crude product, heating to reflux, concentrating, carrying out cooling crystallization, and filtering to obtain the stevioside RA. According to the stevioside RA product obtained by the method, the content of the stevioside RA reaches up to over 98%.
Description
Technical field
The present invention relates to the extraction process for purification of a kind of Steviosides RA.
Background technology
Stevioside is a kind of new type natural sweeting agent that essence is carried from catananche's sweet Stevia, color and luster is pure white, mouthfeel is suitable, free from extraneous odour, being the natural low caloric value sweeting agent that approaches sucrose taste most that the current world has found, is also to continue that the third has exploitation to be worth and healthy natural sucrose substitute of praising highly outside sugarcane, beet sugar, is described as in the world " sugar source, third place in the world ", often edible can preventing hypertension, the disease such as diabetes, obesity, carious tooth, without any side effects.
In Steviosides, have a lot of components, as RA, RC, STV, DA etc., RA content is higher conventionally, illustrates that quality is better.
RA refers to Rebaudioside A, namely A3.RA component is sweet taste component the best in stevioside, by recrystallization technology refining forming from common stevioside.Mouthfeel is pure and fresh in bitter taste.Sugariness be sucrose 300-400 doubly and taste matter closer to sucrose.High temperature resistant, more stable in acidity and basic solution, be highly suitable for bake.Can improve better the mouthfeel of numerous food, improve quality and the class of product.
Yet, at present the method for extraction of steviosides RA, is by sweet Stevia cured leaf is immersed in water, filters liquid and leaf, stem separated, further utilize water or food grade alcohol etc. to purify, in the method gained Steviosides RA product, Steviosides RA content is lower.
Summary of the invention
Technical problem to be solved by this invention is, the extraction process for purification of a kind of Steviosides RA is provided, the Steviosides RA product that adopts the method to obtain, and Steviosides RA content is high.
The technical solution adopted for the present invention to solve the technical problems is: the extraction process for purification of a kind of Steviosides RA, comprises the following steps:
(1) extract: with hot water, as solvent, adopt percolation that the Steviosides RA in raw material sweet Stevia cured leaf is extracted, obtain percolate;
(2) flocculation: regulate pH to be adjusted to 10～11 with lime percolate, add flocculation agent in percolate, the consumption of flocculation agent is 1～5% of raw material sweet Stevia quality, and constantly stirs, and flocculates 1～2 hour, then staticly settles 5～8 hours, and press filtration, obtains filtrate;
(3) chromatography: by the chromatography column of macroporous resin is housed, the consumption of macroporous resin is 0.5～1.5 times of raw material sweet Stevia quality by filtrate, then uses deionized water rinsing post bed, until effluent liquid is colourless, use again ethanol elution, colourless to effluent liquid, collect ethanol eluate;
(4) desalination: ethanol eluate, by the chromatography column of ion exchange resin is housed, was collected to post effluent liquid;
(5) decolouring: add gac toward crossing in post effluent liquid, activated carbon dosage is 0.5～2% of raw material sweet Stevia quality, temperature rising reflux 2～3 hours, and constantly stir, filtered while hot, obtains filtrate;
(6) concentrated: to concentrate filtrate in concentrated solution without ethanol, obtain Steviosides RA crude product;
(7) refining: toward the methyl alcohol that adds volume fraction 60～99% in Steviosides RA crude product, methyl alcohol add-on is 5～10 times of Steviosides RA crude product quality, temperature rising reflux dissolves 2～3 hours, be concentrated into the former volume fraction adding 60～99% methyl alcohol volume 1/2～1/3, then within every two hours, to decline the speed decrease temperature crystalline of 0.8～1.2 ℃, until 10～15 ℃, filter, obtain Steviosides RA product.
Further, in step (1), the temperature of hot water is 60～80 ℃, and using amount of hot water is 15～20 times of raw material sweet Stevia quality, and flow velocity is 10～20L/min.
Further, in step (2), described flocculation agent is Tai-Ace S 150 or iron(ic) chloride etc., in the mode of saturated solution, adds.
Further, in step (3), wash-out is 70～90% by the volume fraction of ethanol.
Further, in step (3), the flow velocity of filtrate absorption upper prop is 1～2BV/ hour, and the flow velocity of deionized water rinsing post bed is 3～5BV/ hour, and the flow velocity of ethanol elution is 2～3BV/ hour.
Further, in step (4), described ion exchange resin is anionite-exchange resin, and consumption is 0.5～1 times of raw material sweet Stevia quality.
Further, in step (4), the flow velocity that ethanol eluate is crossed post is 1～2BV/ hour.
Further, in step (5), described gac is sugaring special-purpose activated charcoal (purchased from filter material company limited of Gongyi City PCI-SLR Technology).
The present invention has the following advantages:
(1) adopt percolation to extract the Steviosides in sweet Stevia, not only can improve extraction yield 5%～10%, also reduce greatly the consumption of water, correspondingly reduce treatment capacity and quantity of wastewater effluent in technological process;
(2) percolate is after flocculation, desalination, bleaching process, and wherein the impurity of the overwhelming majority can be removed, and the content of the crude product Steviosides RA obtaining after concentrating reaches 90%;
(3) crude product refining methanol, in gradient cooling process, Steviosides RA crystal is separated out lentamente, is increased, and in the Steviosides RA product finally obtaining, Steviosides RA content is more than 98%.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
The present embodiment comprises the following steps:
(1) extract: get sweet Stevia cured leaf 100kg, insert in percolate pot, first use 60 ℃ of hot-water soak 30min of 500L, then add 60 ℃ of hot water of 1500L in percolate pot, coutroi velocity 15L/min, obtains percolate;
(2) flocculation: will regulate after pH to 10 with lime in percolate, then add the saturated solution of the Tai-Ace S 150 that has dissolved 3kg, and constantly stir, flocculate 2 hours, then staticly settle 6 hours, press filtration, obtains filtrate;
(3) chromatography: by filtrate by the chromatography column of AB-8 macroporous resin is housed, the consumption of AB-8 macroporous resin is 100kg, the flow velocity of upper prop is 1 BV/ hour, use deionized water rinsing post bed, flow velocity is 3BV/ hour again, until effluent liquid is colourless, then use the ethanol elution of volume fraction 85%, the flow velocity of wash-out is 2BV/ hour, colourless to effluent liquid, collects elutriant 400L;
(4) desalination: by the chromatography column of anionite-exchange resin is housed, the consumption of anionite-exchange resin is 60kg by ethanol eluate, and flow velocity is 1BV/ hour, collects post effluent liquid;
(5) decolouring: add sugaring special-purpose activated charcoal 1kg(purchased from filter material company limited of Gongyi City PCI-SLR Technology toward crossing in post effluent liquid), temperature rising reflux 3 hours, and constantly stir filtered while hot;
(6) concentrated: to concentrate filtrate in concentrated solution without ethanol, obtain Steviosides crude product 4kg, content 91.67%;
(7) refining: toward the methyl alcohol 30L that adds volume fraction 95% in Steviosides crude product, temperature rising reflux dissolving 2 hours, is concentrated into 15L, then within every two hours, to decline the speed decrease temperature crystalline of 1 ℃, until 10 ℃, filter, obtain Steviosides RA product 3.5kg, Steviosides RA content 98.12%.
Embodiment 2
The present embodiment comprises the following steps:
(1) extract: get sweet Stevia cured leaf 200kg, insert in percolate pot, first use 60 ℃ of hot-water soak 30min of 1000L, then add 60 ℃ of hot water of 2200L in percolate pot, coutroi velocity 15L/min, obtains percolate;
(2) flocculation: percolate is regulated after pH to 10 with lime, then add the saturated solution of the Tai-Ace S 150 that has dissolved 6kg, and constantly stir, flocculate 1.5 hours, then staticly settle 7 hours, press filtration, obtains filtrate;
(3) chromatography: by the chromatography column of AB-8 macroporous resin is housed, the consumption of AB-8 macroporous resin is 200kg by filtrate, and the flow velocity of upper prop is 1.2 BV/ hour, then uses deionized water rinsing post bed, and flow velocity is 3BV/ hour, until effluent liquid is colourless; Then use the ethanol elution of volume fraction 85%, the flow velocity of wash-out is 2.5BV/ hour, colourless to effluent liquid, collects elutriant 800L;
(4) desalination: by the chromatography column of anionite-exchange resin is housed, the consumption of anionite-exchange resin is 120kg by ethanol eluate, and flow velocity is 2BV/ hour, collects post effluent liquid;
(5) decolouring: add sugaring special-purpose activated charcoal 2kg(purchased from filter material company limited of Gongyi City PCI-SLR Technology toward crossing in post effluent liquid), temperature rising reflux 2 hours, and constantly stir filtered while hot;
(6) concentrated: to concentrate filtrate in concentrated solution without ethanol, obtain Steviosides crude product 8.2kg, content 91.16%;
(7) refining: toward the methyl alcohol 70L that adds volume fraction 95% in Steviosides crude product, temperature rising reflux dissolving 2 hours, is concentrated into 35L, then within every two hours, to decline the speed decrease temperature crystalline of 1 ℃, until 10 ℃, filter, obtain Steviosides RA product 5.9kg, Steviosides RA content 98.68%.
Claims (8)
1. an extraction process for purification of Steviosides RA, is characterized in that, comprises the following steps:
(1) extract: with hot water, as solvent, adopt percolation that the Steviosides RA in raw material sweet Stevia cured leaf is extracted, obtain percolate;
(2) flocculation: regulate pH to be adjusted to 10～11 with lime percolate, add flocculation agent in percolate, the consumption of flocculation agent is 1～5% of raw material sweet Stevia quality, and constantly stirs, and flocculates 1～2 hour, then staticly settles 5～8 hours, and press filtration, obtains filtrate;
(3) chromatography: by the chromatography column of macroporous resin is housed, the consumption of macroporous resin is 0.5～1.5 times of raw material sweet Stevia quality by filtrate, then uses deionized water rinsing post bed, until effluent liquid is colourless, use again ethanol elution, colourless to effluent liquid, collect ethanol eluate;
(4) desalination: ethanol eluate, by the chromatography column of ion exchange resin is housed, was collected to post effluent liquid;
(5) decolouring: add gac toward crossing in post effluent liquid, activated carbon dosage is 0.5～2% of raw material sweet Stevia quality, temperature rising reflux 2～3 hours, and constantly stir, filtered while hot, obtains filtrate;
(6) concentrated: to concentrate filtrate in concentrated solution without ethanol, obtain Steviosides RA crude product;
(7) refining: toward the methyl alcohol that adds volume fraction 60～99% in Steviosides RA crude product, methyl alcohol add-on is 5～10 times of Steviosides RA crude product quality, temperature rising reflux dissolves 2～3 hours, be concentrated into the former volume fraction adding 60～99% methyl alcohol volume 1/2～1/3, then within every two hours, to decline the speed decrease temperature crystalline of 0.8～1.2 ℃, until 10～15 ℃, filter, obtain Steviosides RA product.
2. the extraction process for purification of Steviosides RA according to claim 1, is characterized in that, in step (1), the temperature of hot water is 60～80 ℃, and using amount of hot water is 15～20 times of raw material sweet Stevia quality, and flow velocity is 10～20L/min.
3. the extraction process for purification of Steviosides RA according to claim 1 and 2, is characterized in that, in step (2), described flocculation agent is Tai-Ace S 150 or iron(ic) chloride, in the mode of saturated solution, adds.
4. the extraction process for purification of Steviosides RA according to claim 1 and 2, is characterized in that, in step (3), wash-out is 70～90% by the volume fraction of ethanol.
5. the extraction process for purification of Steviosides RA according to claim 1 and 2, it is characterized in that, in step (3), the flow velocity of filtrate absorption upper prop is 1～2BV/ hour, the flow velocity of deionized water rinsing post bed is 3～5BV/ hour, and the flow velocity of ethanol elution is 2～3BV/ hour.
6. the extraction process for purification of Steviosides RA according to claim 1 and 2, is characterized in that, in step (4), described ion exchange resin is anionite-exchange resin, and consumption is 0.5～1 times of raw material sweet Stevia quality.
7. the extraction process for purification of Steviosides RA according to claim 1 and 2, is characterized in that, in step (4), the flow velocity that ethanol eluate is crossed post is 1～2BV/ hour.
8. the extraction process for purification of Steviosides RA according to claim 1 and 2, is characterized in that, in step (5), described gac is sugaring special-purpose activated charcoal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310531197.7A CN103570777A (en) | 2013-11-01 | 2013-11-01 | Extraction and purification method of stevioside RA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310531197.7A CN103570777A (en) | 2013-11-01 | 2013-11-01 | Extraction and purification method of stevioside RA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103570777A true CN103570777A (en) | 2014-02-12 |
Family
ID=50043553
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310531197.7A Pending CN103570777A (en) | 2013-11-01 | 2013-11-01 | Extraction and purification method of stevioside RA |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103570777A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105017348A (en) * | 2015-08-13 | 2015-11-04 | 青岛润德生物科技有限公司 | Method for extracting stevioside from biological enzyme fermentation liquid |
| CN105175462A (en) * | 2015-08-31 | 2015-12-23 | 湖南威嘉生物科技有限公司 | Process for extracting stevioside by percolation method |
| CN105732739A (en) * | 2016-01-29 | 2016-07-06 | 滁州润海甜叶菊高科有限公司 | Technique for extracting and purifying stevioside from stevia |
| CN107118243A (en) * | 2017-06-05 | 2017-09-01 | 江苏史蒂文生物科技有限公司 | A kind of industrial production process of stevioside |
| CN107325137A (en) * | 2017-08-21 | 2017-11-07 | 内蒙古昶辉生物科技股份有限公司 | A kind of extracting method of steviol glycoside |
| CN110437290A (en) * | 2019-06-14 | 2019-11-12 | 中国林业科学研究院林产化学工业研究所 | A kind of steviol glycoside extracting and developing and purification process |
| CN110776537A (en) * | 2019-10-18 | 2020-02-11 | 临夏回族自治州林业和草原局 | A method for preparing stevioside or RA from stevia rebaudiana Bertoni |
| WO2021216553A1 (en) * | 2020-04-20 | 2021-10-28 | Cargill, Incorporated | Increasing yield of steviol glycosides |
| CN114621305A (en) * | 2022-03-22 | 2022-06-14 | 咸阳师范学院 | Extraction and refining process of stevioside |
| US11944112B2 (en) | 2020-04-20 | 2024-04-02 | Cargill, Incorporated | Stabilized steviol glycoside malonic acid esters |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5262300A (en) * | 1975-11-13 | 1977-05-23 | Ajinomoto Co Inc | Isolation of bibaudioside-a by crystallization |
| JPH06192283A (en) * | 1992-12-17 | 1994-07-12 | Ikeda Pan:Kk | Method for production pure rebaudioside a |
| CN101200480A (en) * | 2006-12-15 | 2008-06-18 | 成都华高药业有限公司 | Rebaudioside A and extraction method thereof |
| CN101220062A (en) * | 2008-01-23 | 2008-07-16 | 石任兵 | Method for preparing stevioside and rebaudiodside A simultaneously |
| CN101270138A (en) * | 2007-03-23 | 2008-09-24 | 宁波绿之健药业有限公司 | Extracting method for high-content sweet inulin A3 |
| CN101830947A (en) * | 2010-05-05 | 2010-09-15 | 宋英海 | Method for making high-content rebaudioside-A stevioside |
| US20110124587A1 (en) * | 2004-10-18 | 2011-05-26 | SGF Holdings, LLC | Pure Rebaudioside A |
| CN102127129A (en) * | 2010-12-13 | 2011-07-20 | 辽宁千千生物科技有限公司 | Method for extracting stevioside from fresh stevia rebaudiana leaves |
| CN102216313A (en) * | 2008-10-03 | 2011-10-12 | 守田化学工业株式会社 | New steviol glycoside |
-
2013
- 2013-11-01 CN CN201310531197.7A patent/CN103570777A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5262300A (en) * | 1975-11-13 | 1977-05-23 | Ajinomoto Co Inc | Isolation of bibaudioside-a by crystallization |
| JPH06192283A (en) * | 1992-12-17 | 1994-07-12 | Ikeda Pan:Kk | Method for production pure rebaudioside a |
| US20110124587A1 (en) * | 2004-10-18 | 2011-05-26 | SGF Holdings, LLC | Pure Rebaudioside A |
| CN101200480A (en) * | 2006-12-15 | 2008-06-18 | 成都华高药业有限公司 | Rebaudioside A and extraction method thereof |
| CN101270138A (en) * | 2007-03-23 | 2008-09-24 | 宁波绿之健药业有限公司 | Extracting method for high-content sweet inulin A3 |
| CN101220062A (en) * | 2008-01-23 | 2008-07-16 | 石任兵 | Method for preparing stevioside and rebaudiodside A simultaneously |
| CN102216313A (en) * | 2008-10-03 | 2011-10-12 | 守田化学工业株式会社 | New steviol glycoside |
| CN101830947A (en) * | 2010-05-05 | 2010-09-15 | 宋英海 | Method for making high-content rebaudioside-A stevioside |
| CN102127129A (en) * | 2010-12-13 | 2011-07-20 | 辽宁千千生物科技有限公司 | Method for extracting stevioside from fresh stevia rebaudiana leaves |
Non-Patent Citations (3)
| Title |
|---|
| 张亚雄等: "甜菊糖中A_3甙的分离提纯", 《氨基酸和生物资源》 * |
| 李洪飞等: "甜叶菊甙提取液絮凝工艺技术研究", 《中国食品学报》 * |
| 赵昊等: "溶析结晶法分离莱鲍迪甙A的工艺研究", 《应用化工》 * |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105017348B (en) * | 2015-08-13 | 2018-01-23 | 青岛润德生物科技有限公司 | A kind of method that steviol glycoside is extracted in the liquid from fermented by biological enzyme |
| CN105017348A (en) * | 2015-08-13 | 2015-11-04 | 青岛润德生物科技有限公司 | Method for extracting stevioside from biological enzyme fermentation liquid |
| CN105175462A (en) * | 2015-08-31 | 2015-12-23 | 湖南威嘉生物科技有限公司 | Process for extracting stevioside by percolation method |
| CN105175462B (en) * | 2015-08-31 | 2018-05-22 | 湖南威嘉生物科技有限公司 | A kind of technique of percolation extraction stevioside |
| CN105732739A (en) * | 2016-01-29 | 2016-07-06 | 滁州润海甜叶菊高科有限公司 | Technique for extracting and purifying stevioside from stevia |
| CN107118243A (en) * | 2017-06-05 | 2017-09-01 | 江苏史蒂文生物科技有限公司 | A kind of industrial production process of stevioside |
| CN107118243B (en) * | 2017-06-05 | 2020-06-19 | 江苏史蒂文生物科技有限公司 | Industrial preparation method of stevioside |
| CN107325137A (en) * | 2017-08-21 | 2017-11-07 | 内蒙古昶辉生物科技股份有限公司 | A kind of extracting method of steviol glycoside |
| CN110437290A (en) * | 2019-06-14 | 2019-11-12 | 中国林业科学研究院林产化学工业研究所 | A kind of steviol glycoside extracting and developing and purification process |
| CN110776537A (en) * | 2019-10-18 | 2020-02-11 | 临夏回族自治州林业和草原局 | A method for preparing stevioside or RA from stevia rebaudiana Bertoni |
| CN110776537B (en) * | 2019-10-18 | 2022-09-06 | 临夏回族自治州林业和草原局 | A method for preparing stevioside or RA from stevia rebaudiana Bertoni |
| WO2021216553A1 (en) * | 2020-04-20 | 2021-10-28 | Cargill, Incorporated | Increasing yield of steviol glycosides |
| CN115666269A (en) * | 2020-04-20 | 2023-01-31 | 嘉吉公司 | Increasing yield of steviol glycosides |
| US11944112B2 (en) | 2020-04-20 | 2024-04-02 | Cargill, Incorporated | Stabilized steviol glycoside malonic acid esters |
| CN114621305A (en) * | 2022-03-22 | 2022-06-14 | 咸阳师范学院 | Extraction and refining process of stevioside |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103570777A (en) | Extraction and purification method of stevioside RA | |
| CN101717418B (en) | Technology for separating stevioside in one step with simulated moving bed | |
| CN108516997B (en) | Method for extracting rubusoside from sweet tea leaves | |
| CN108752231B (en) | Method for extracting theanine from sweet tea and simultaneously extracting rubusoside and tea polyphenol | |
| CN107325137B (en) | Extraction method of stevioside | |
| CN102060892A (en) | Method for purifying stevioside RD (Rebaudioside D) | |
| CN102924544B (en) | Method for preparing stevioside and chlorogenic acid from stevia step by step | |
| CN101805768B (en) | Biological enzymolysis and purification method of high-quality stevioside | |
| CN102199177A (en) | Manufacturing method of pure natural high-purity stevioside | |
| WO2020063894A1 (en) | Industrial utilization method for stevia rebaudiana and stevioside and chlorogenic acid of stevia rebaudiana | |
| CN101235062A (en) | Preparation technique for extracting high pure hesperidin by cold impurity-removing one-step method | |
| CN105175462A (en) | Process for extracting stevioside by percolation method | |
| CN101759689A (en) | Preparation method of mangiferin | |
| CN102977163A (en) | Industrialized preparation method of stevioside | |
| CN113501759A (en) | Method for obtaining chlorogenic acid and isochlorogenic acid from stevia rebaudiana residue | |
| CN104017028B (en) | The method separating isomaltulose and trehalulose from isomaltulose mother solution | |
| CN108117571B (en) | Preparation method of gentiopicroside monomer | |
| CN110078775B (en) | Environment-friendly production method of high-content rubusoside and rubuspolyphenol | |
| CN110437290A (en) | A kind of steviol glycoside extracting and developing and purification process | |
| CN111056941B (en) | Method for preparing high-purity shikimic acid by utilizing ginkgo leaf extract chromatography waste liquid | |
| JP2018108982A (en) | Extraction and separation method of eurycomanone from root of tongkut ali | |
| CN112266399A (en) | High-purity separation and extraction method of epimedium extract | |
| CN106831906A (en) | A kind of ultrasonic wave added method extraction of steviosides from STEVIA REBAUDIANA | |
| CN110776537B (en) | A method for preparing stevioside or RA from stevia rebaudiana Bertoni | |
| CN101475618A (en) | Refining method for scutelloside for injection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140212 |