CN103566023B - A kind of preparation method of toothache Pianomide and application - Google Patents
A kind of preparation method of toothache Pianomide and application Download PDFInfo
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Abstract
The invention belongs to technical field of Chinese medicines, be specifically related to a kind of preparation method and application of toothache Pianomide.The preparation method of toothache Pianomide of the present invention, be made up as crude drug of Gypsum Fibrosum 200g, Herba Schizonepetae 80g, Radix Saposhnikoviae 80g, Pericarpium Citri Reticulatae Viride 100g, Cortex Moutan 100g, Radix Rehmanniae 150g, Indigo Naturalis 100g, Herba Asari 60g, Radix Angelicae Dahuricae 50g, Radix Glycyrrhizae 60g, adopt supercritical extraction and microwave extracting to be prepared from, content of hesperidin is improved a lot.Present invention also offers the application of toothache Pianomide in preparation suppression mouse leukemia cell L1210 cell proliferation.
Description
Technical field
The invention belongs to technical field of Chinese medicines, be specifically related to a kind of preparation method and application of toothache Pianomide.
Background technology
Toothache antiphlogistic granules is recorded in Traditional Chinese medicine historical preparation the tenth the 85th page and Chinese Pharmacopoeia version in 2010 the 807th page, be made up as crude drug of Gypsum Fibrosum 200g, Herba Schizonepetae 80g, Radix Saposhnikoviae 80g, Pericarpium Citri Reticulatae Viride 100g, Cortex Moutan 100g, Radix Rehmanniae 150g, Indigo Naturalis 100g, Herba Asari 60g, Radix Angelicae Dahuricae 50g, Radix Glycyrrhizae 60g, there is wind and heat dispersing, effect of cooling blood and relieving pain.Caused headache fever of the body, xerostomia halitosis, constipation dryness accumulated in the stomach and intestine, gingival swelling and pain is attacked in retention of heat in the spleen and stomach, wind heat; Acute tooth root periapical periodontitis, pericoronitis of the wisdom tooth, acute gingiva (week) are scorching, acute pulpitis is shown in above-mentioned patient.
In prior art, toothache antiphlogistic granules is not yet had to extract the report adopting supercritical and microwave technology in preparation, and volatile oil is drawn, dipping diafiltration, the method of soak by water, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and applied clinically.
In prior art, each 1 bag of toothache antiphlogistic granules, every bag of 20g(has sugar) or 10g(sugar-free), 3 times on the one, double first.Toothache antiphlogistic granules dosage is large, and more bitter under the state of not sugaring, and under patient is not easy clothes, compliance is poor.The every sheet 0.8g of toothache Pianomide adopting the inventive method to be prepared into, only needs 2 at every turn, within 1st, takes 3 times, double first.Dose is greatly reduced under the condition with more active component.This conclusion can be proved by following test.And being prepared into tablet, patient swallows with water, and dosage is little and without bitterness, patient is easy to accept.
The comparison of content of hesperidin in toothache Pianomide prepared by test one, distinct methods
L, instrument and reagent toothache Pianomide of the present invention: by the preparation of embodiment 1 method, use 980g crude drug, makes 100 through extracting, the heavy 0.8g of every sheet.Former toothache antiphlogistic granules, by 2010 editions standards of pharmacopoeia method preparations, using 980g crude drug, making 50 bags through extracting, every bag heavy 10g.Agilent1200 high performance liquid chromatograph; METTLERAE240 electronic analytical balance; Hesperidin reference substance (Nat'l Pharmaceutical & Biological Products Control Institute).
2, method
Measure according to high performance liquid chromatography (annex VD).
Chromatographic condition and system suitability take octadecylsilane chemically bonded silica as filler; With methanol-water (35:65) for mobile phase; Determined wavelength is 283nm.Number of theoretical plate calculates should be not less than 1000 by Hesperidin peak.
The preparation of reference substance solution gets Hesperidin reference substance in right amount, accurately weighed, adds methanol and makes the solution of every 1ml containing 80 μ g, to obtain final product.
Toothache Pianomide of the present invention is got in the preparation of product need testing solution of the present invention, and porphyrize, gets 0.16g, accurately weighed, put in tool plug conical flask, precision adds the close plug of methanol 25ml., weighed weight, supersound process (power 250w. frequency 33kHz) 1 hour, lets cool, weighed weight again, supply the weight of less loss with methanol, shake up, filter, get subsequent filtrate, to obtain final product.
The toothache antiphlogistic granules of this contrast is got in the preparation of reference product need testing solution, and porphyrize, gets 1g, accurately weighed, put in tool plug conical flask, precision adds the close plug of methanol 25ml., weighed weight, supersound process (power 250w. frequency 33kHz) 1 hour, lets cool, weighed weight again, supply the weight of less loss with methanol, shake up, filter, get subsequent filtrate, to obtain final product.
Algoscopy is accurate respectively draws reference substance solution and each 20 μ l. injection liquid chromatographies of need testing solution, measures, to obtain final product.
3, result
Result shows, in toothache Pianomide of the present invention, the content of Hesperidin is 75-90mg/ sheet; And the content of Hesperidin is 16.2mg/ bag in former toothache antiphlogistic granules, when dose reduces, content of hesperidin improves a lot.
Above-mentioned research shows, adopts toothache Pianomide prepared by preparation method of the present invention, and active constituent content is far away higher than toothache antiphlogistic granules prepared by the method recorded in 2010 editions standards of pharmacopoeia.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of preparation method of toothache Pianomide.
Another object of the present invention is to provide a kind of toothache Pianomide to suppress the application in mouse leukemia cell L1210 cell proliferation in preparation.
The object of the invention is by following scheme realize:
A kind of preparation method of toothache Pianomide, be made up as crude drug of Gypsum Fibrosum 200g, Herba Schizonepetae 80g, Radix Saposhnikoviae 80g, Pericarpium Citri Reticulatae Viride 100g, Cortex Moutan 100g, Radix Rehmanniae 150g, Indigo Naturalis 100g, Herba Asari 60g, Radix Angelicae Dahuricae 50g, Radix Glycyrrhizae 60g, described preparation method is made up of the following step: get Herba Schizonepetae, Herba Asari, the Radix Angelicae Dahuricae, Indigo Naturalis, join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO
2flow l-3ml/g crude drug min, extraction time 180-220min, obtains supercritical extract, for subsequent use; Get all the other Chinese medicines, pulverize, add 50% ethanol of 1.6L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 600-800W, extracts 2 times, each 5-10 minute, combining extraction liquid, concentrated, be added on Dl01 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, for subsequent use; By above-mentioned supercritical extract and the mixing of microwave extraction thing, add starch, 70% ethanol granule, dry, tabletting, makes 100, the heavy 0.8g of every sheet.
In the preparation method of above-mentioned toothache Pianomide, described CO
2in supercritical extraction, entrainer accounts for the percent by volume of total extractant is 5%.
In the preparation method of above-mentioned toothache Pianomide, described CO
2in supercritical extraction, extracting pressure is 25MPa.
In the preparation method of above-mentioned toothache Pianomide, described CO
2in supercritical extraction, extraction temperature is 60 DEG C.
In the preparation method of above-mentioned toothache Pianomide, described CO
2cO in supercritical extraction
2flow 2ml/g crude drug min.
In the preparation method of above-mentioned toothache Pianomide, described CO
2in supercritical extraction, extraction time is 200min.
In the preparation method of above-mentioned toothache Pianomide, described microwave extracting power is 700W.
In the preparation method of above-mentioned toothache Pianomide, each extraction time of described microwave extracting is 8 minutes.
Above-mentioned toothache Pianomide suppresses the application in mouse leukemia cell L1210 cell proliferation in preparation.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following example, all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Gypsum Fibrosum 200g, Herba Schizonepetae 80g, Radix Saposhnikoviae 80g, Pericarpium Citri Reticulatae Viride 100g, Cortex Moutan 100g, Radix Rehmanniae 150g, Indigo Naturalis 100g, Herba Asari 60g, Radix Angelicae Dahuricae 50g, Radix Glycyrrhizae 60g, Herba Schizonepetae, Herba Asari, the Radix Angelicae Dahuricae, Indigo Naturalis are joined CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 5%, extracting pressure 25MPa, temperature 40 DEG C, CO
2flow 2ml/g crude drug min, extraction time 200min, obtains supercritical extract, for subsequent use; Get all the other Chinese medicines, pulverize, add 50% ethanol of 1.6L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 700W, extracts 2 times, each 8 minutes, combining extraction liquid, concentrated, be added on Dl01 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, for subsequent use; By above-mentioned supercritical extract and the mixing of microwave extraction thing, add starch, 70% ethanol granule, dry, tabletting, makes 100, the heavy 0.8g of every sheet.
After testing, in finished product, the content of Hesperidin is 88.5mg/ sheet.
Embodiment 2
Get Gypsum Fibrosum 200g, Herba Schizonepetae 80g, Radix Saposhnikoviae 80g, Pericarpium Citri Reticulatae Viride 100g, Cortex Moutan 100g, Radix Rehmanniae 150g, Indigo Naturalis 100g, Herba Asari 60g, Radix Angelicae Dahuricae 50g, Radix Glycyrrhizae 60g, Herba Schizonepetae, Herba Asari, the Radix Angelicae Dahuricae, Indigo Naturalis are joined CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4%, extracting pressure 30MPa, temperature 50 C, CO
2flow 3ml/g crude drug min, extraction time 180min, obtains supercritical extract, for subsequent use; Get all the other Chinese medicines, pulverize, add 50% ethanol of 1.6L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 600W, extracts 2 times, each 5 minutes, combining extraction liquid, concentrated, be added on Dl01 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, for subsequent use; By above-mentioned supercritical extract and the mixing of microwave extraction thing, add starch, 70% ethanol granule, dry, tabletting, makes 100, the heavy 0.8g of every sheet.
After testing, in finished product, the content of Hesperidin is 76.2mg/ sheet.
Embodiment 3
Get Gypsum Fibrosum 200g, Herba Schizonepetae 80g, Radix Saposhnikoviae 80g, Pericarpium Citri Reticulatae Viride 100g, Cortex Moutan 100g, Radix Rehmanniae 150g, Indigo Naturalis 100g, Herba Asari 60g, Radix Angelicae Dahuricae 50g, Radix Glycyrrhizae 60g, Herba Schizonepetae, Herba Asari, the Radix Angelicae Dahuricae, Indigo Naturalis are joined CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 6%, extracting pressure 15MPa, temperature 30 DEG C, CO
2flow 1ml/g crude drug min, extraction time 220min, obtains supercritical extract, for subsequent use; Get all the other Chinese medicines, pulverize, add 50% ethanol of 1.6L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 800W, extracts 2 times, each 10 minutes, combining extraction liquid, concentrated, be added on Dl01 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, for subsequent use; By above-mentioned supercritical extract and the mixing of microwave extraction thing, add starch, 70% ethanol granule, dry, tabletting, makes 100, the heavy 0.8g of every sheet.
After testing, in finished product, the content of Hesperidin is 78.5mg/ sheet.
Embodiment 4: toothache Pianomide suppresses the experimentation data of mouse leukemia cell L1210 cell proliferation
1. experiment material
1.1 experiment cell strains
Mouse leukemia cell L1210 cell, laboratory cell storehouse, Shandong Prov. Hospital, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: toothache Pianomide of the present invention: prepare by embodiment 1 method.
Medicinal liquid liquid storage: take 100mg toothache Pianomide, is dissolved in 5ml dehydrated alcohol, 0.2 μm of frit, 500 μ ldoff pipe subpackages ,-20 DEG C of storages, and simultaneously 0.2 μm of frit dehydrated alcohol is in order to the use of matched group.
1.3 experiment reagent
DMEM (GIBCO company Cat.No.12100-061Lot.No.758137); Hyclone (Tian Hang bio tech ltd, Zhejiang Lot.No.100419); NaHC0
3(Shanghai hundred million chemical reagent company limited Cat.No.11810-033Lot.No.1088387 of a specified duration); Trypsin(AMRESCO company 1); EDTA(AMRESCO company 1); PenicillinGSodiumSalt(AMRESCO company 1); StreptomycinSulfate (AMRESCO); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.); MTT (Biosharp lot number: 0793): PBS(laboratory autogamy);
1.4 experiment equipment
Lycra inverted microscope (German Leica model: DMIL); Visible-ultraviolet light microwell plate detector (MD company of U.S. model: SPECTRAMAX190); C0
2incubator (FORMA model: 3111); Super-clean bench (safe and sound Inc. of Su Jing group moulding number: SW-CJ-ZFD); Pure water instrument (Sprlng company of U.S. model: S/N020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (the accurate experimental facilities company in Shanghai model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μm of filter (MILLIPORE model: SLGP033RB); 1cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2. experimental technique
1) L1210 cell DMEM+10%FBS is in 37 DEG C, 5%C0
2carry out cellar culture (10cm culture dish), when Growth of Cells is to logarithmic (log) phase, collecting cell, discards culture fluid, PBS fine laundering 3 times, add 3ml0.25% trypsin-0.04%EDTA, after 37 DEG C of digestion 2min, add 5ml complete medium neutralization reaction wherein, proceeded in centrifuge tube after piping and druming cell, the centrifugal 5min of 1000rpm, adjustment concentration of cell suspension 3 × 10
4individual/ml.
2) enter in 96 well culture plates by cell kind, every hole adds cell suspension 180 μ l, culture plate put into cell culture incubator (37 DEG C, 5%C0
2) cellar culture.
3) according to cell growth status, generally grow to 50%-70%, add toothache Pianomide solution, continue to cultivate 24h.
4) add 20 μ lMTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.
5) after 4h, buckle method removes supernatant, pats dry gently with absorbent paper, and every hole, as entered 200 μ l dimethyl sulfoxide, is put low-speed oscillation 10min on shaking table, crystal is fully dissolved.The light absorption value in each hole is measured at enzyme-linked immunosorbent assay instrument 490nm place.
6) background (do not add cell, only add culture fluid) is set, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide) simultaneously, often organizes the multiple hole of setting 6.
7) result represents with the suppression ratio of medicine to cell: cell proliferation suppression ratio (%)=(control wells OD value-dosing holes OD value), control wells OD value × 100%.Experiment repetition 3 times.
3. statistical disposition
Adopt the correlation analysis in MicrosoftExcel2007 software and Studentt inspection, data represent with mean ± S.D..
4. experimental result
Statistical result showed after mtt assay experiment, compare with matched group, when dosage reaches 5mg/ml, to L1210 cell inhibitory effect variant (P<0.05), dosage this difference when 10mg/ml has significance (P<0.01), has pole significant difference (P<0.001) when dosage reaches 15-20mg/ml.
Table 1 toothache Pianomide is to L1210 cell inhibitory effect influence research (X ± SD)
| Group | Drug level (mg/ml) | Suppression ratio (%) |
| Matched group | 0 | 0 |
| 1 | 5 | 14.5±11.52 |
| 2 | 10 | 24.2±13.68* |
| 3 | 15 | 37.5±15.27** |
| 4 | 20 | 42.8±14.96** |
Note: compare with matched group, * P<0.01; * P<0.001.
5. experiment conclusion
Toothache Pianomide can suppress L1210 cell proliferation, and reduce the Growth of Cells number of L1210 cell, this effect is dose dependent.
Claims (7)
1. the application of toothache Pianomide in preparation suppression mouse leukemia cell L1210 cell proliferation, it is characterized in that, described toothache Pianomide is made up as crude drug of Gypsum Fibrosum 200g, Herba Schizonepetae 80g, Radix Saposhnikoviae 80g, Pericarpium Citri Reticulatae Viride 100g, Cortex Moutan 100g, Radix Rehmanniae 150g, Indigo Naturalis 100g, Herba Asari 60g, Radix Angelicae Dahuricae 50g, Radix Glycyrrhizae 60g, the preparation method of described toothache Pianomide is made up of the following step: get Herba Schizonepetae, Herba Asari, the Radix Angelicae Dahuricae, Indigo Naturalis, join CO
2in supercritical extraction device, ethanol is as entrainer, and the percent by volume that entrainer accounts for total extractant is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO
2flow l-3ml/g crude drug min, extraction time 180-220min, obtains supercritical extract, for subsequent use; Get all the other Chinese medicines, pulverize, add 50% ethanol of 1.6L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 600-800W, extracts 2 times, each 5-10 minute, combining extraction liquid, concentrated, be added on Dl01 macroporous adsorptive resins, 50% ethanol elution, collect 5 times amount column volume eluents, decompression recycling ethanol, concentrated and dry, obtain microwave extraction thing, for subsequent use; By above-mentioned supercritical extract and the mixing of microwave extraction thing, add starch, 70% ethanol granule, dry, tabletting, makes 100, the heavy 0.8g of every sheet.
2. the toothache Pianomide according to claim l suppresses the application in mouse leukemia cell L1210 cell proliferation in preparation, it is characterized in that, described CO
2in supercritical extraction, entrainer accounts for the percent by volume of total extractant is 5%.
3. the toothache Pianomide according to claim l suppresses the application in mouse leukemia cell L1210 cell proliferation in preparation, it is characterized in that, described CO
2in supercritical extraction, extracting pressure is 25MPa.
4. the toothache Pianomide according to claim l suppresses the application in mouse leukemia cell L1210 cell proliferation in preparation, it is characterized in that, described CO
2cO in supercritical extraction
2flow 2ml/g crude drug min.
5. the toothache Pianomide according to claim l suppresses the application in mouse leukemia cell L1210 cell proliferation in preparation, it is characterized in that, described CO
2in supercritical extraction, extraction time is 200min.
6. the toothache Pianomide according to claim l suppresses the application in mouse leukemia cell L1210 cell proliferation in preparation, and it is characterized in that, described microwave extracting power is 700W.
7. the toothache Pianomide according to claim l suppresses the application in mouse leukemia cell L1210 cell proliferation in preparation, and it is characterized in that, each extraction time of described microwave extracting is 8 minutes.
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| CN105726668A (en) * | 2016-03-28 | 2016-07-06 | 济南新时代医药科技有限公司 | Application of lawn pennywort herb Yugan tablet to preparation of drugs for inhibiting small cell lung cancer cell LTEP-P proliferation |
| CN105687356A (en) * | 2016-03-28 | 2016-06-22 | 济南新时代医药科技有限公司 | Application of herba hydrocotylis Yugan tablets in preparing medicine for inhibiting cell proliferation of bladder squamous carcinoma cell SCaBER |
| CN105596449A (en) * | 2016-03-28 | 2016-05-25 | 济南新时代医药科技有限公司 | Application of herba hydrocotylis liver-curing tablets in preparation of medicine for inhibiting cell proliferation of ovarian carcinoma cell CoC2 |
| CN105796668A (en) * | 2016-03-28 | 2016-07-27 | 济南新时代医药科技有限公司 | Application of lawn pennywort herb hepatitis treating tablets in preparing medicine for restraining ovarian adenocarcinoma cell SK-OV-3 proliferation |
| CN105770030A (en) * | 2016-03-28 | 2016-07-20 | 济南新时代医药科技有限公司 | Application of hydrocotyle sibthorpioides herb liver healing tablet in preparation of medicament for inhibiting cell proliferation of laryngeal epidermoid carcinoma cells HEp-2 |
| CN105770029A (en) * | 2016-03-28 | 2016-07-20 | 济南新时代医药科技有限公司 | Application of lawn pennywort herb liver healing tablet in preparation of medicine for inhibiting proliferation of cutaneous melanoma cells A375 |
| CN105816535A (en) * | 2016-03-28 | 2016-08-03 | 济南新时代医药科技有限公司 | Application of lawn pennywort herb liver-healing tablets in preparation of drug for inhibiting cell proliferation of osteosarcoma cells HOS |
| CN105687357A (en) * | 2016-03-28 | 2016-06-22 | 济南新时代医药科技有限公司 | Application of herba hydrocotylis Yugan tablets in preparing medicine for inhibiting the cell proliferation of malignant pleural mesothelioma cell SMC-1 |
| CN105708912A (en) * | 2016-03-28 | 2016-06-29 | 济南新时代医药科技有限公司 | Application of lawn pennywort herb Yugan tablets in preparation of drug for inhibiting proliferation of fibrosarcoma cell HT1080 |
| CN105726670A (en) * | 2016-03-28 | 2016-07-06 | 济南新时代医药科技有限公司 | Application of lawn pennywort herb Yugan tablet to preparation of drugs for inhibiting oral epidermoid carcinoma cell KB proliferation |
| CN105770028A (en) * | 2016-03-28 | 2016-07-20 | 济南新时代医药科技有限公司 | Application of lawn pennywort herb liver healing tablet in preparation of medicine for inhibiting proliferation of colonic adenocarcinoma cells HT-29 |
| CN105816536A (en) * | 2016-03-28 | 2016-08-03 | 济南新时代医药科技有限公司 | Application of lawn pennywort herb liver-healing tablets in preparation of drug for inhibiting cell proliferation of cerebral tumor cells SF767 |
| CN105596451A (en) * | 2016-03-28 | 2016-05-25 | 济南新时代医药科技有限公司 | Application of herba hydrocotylis liver-curing tablets in preparation of medicine for inhibiting cell proliferation of melanoma cell A875 |
| CN105616563A (en) * | 2016-03-28 | 2016-06-01 | 济南新时代医药科技有限公司 | Application of Tianhusui Yugan tablet to preparation of cell proliferation drug for restraining lymphoma cells RAMOS B |
| CN105796661A (en) * | 2016-03-30 | 2016-07-27 | 济南新时代医药科技有限公司 | Application of six-flavor pomegranate tablets to preparation of drug for restraining T cell leukemia cell HPB-ALL cell proliferation |
| CN105832825A (en) * | 2016-03-30 | 2016-08-10 | 济南新时代医药科技有限公司 | Use of tablet of six ingredients with pomegranate seeds in preparation of drug for inhibiting brain tumor cell SF126 proliferation |
| CN105816530A (en) * | 2016-03-30 | 2016-08-03 | 济南新时代医药科技有限公司 | Application of six-ingredient pomegranate tablets in preparation of drug for inhibiting cell proliferation of skin basal cell carcinoma cells A431 |
| CN105726649A (en) * | 2016-03-30 | 2016-07-06 | 济南新时代医药科技有限公司 | Application of Liuweishiliu tablet to preparation of drugs for inhibiting uterine well-differentiated squamous carcinoma cell HCC proliferation |
| CN105796660A (en) * | 2016-03-30 | 2016-07-27 | 济南新时代医药科技有限公司 | Application of six-flavor pomegranate tablets to preparation of drug for restraining neurogliocytoma cell U251 cell proliferation |
| CN105726650A (en) * | 2016-03-30 | 2016-07-06 | 济南新时代医药科技有限公司 | Application of Liuweishiliu tablet to preparation of drugs for inhibiting malignant brain glioma cell SHG-44 proliferation |
| CN105616523A (en) * | 2016-03-30 | 2016-06-01 | 济南新时代医药科技有限公司 | Application of six-flavor pomegranate tablet to preparation of cell proliferation drug for restraining neuroblastoma cells M17 |
| CN105616524A (en) * | 2016-03-30 | 2016-06-01 | 济南新时代医药科技有限公司 | Application of six-flavor pomegranate tablet to preparation of cell proliferation drug for restraining B lymphocyte tumor cells Daudi |
| CN105726651A (en) * | 2016-03-30 | 2016-07-06 | 济南新时代医药科技有限公司 | Application of Liuweishiliu tablet to preparation of drugs for inhibiting cutaneous melanoma cell SK-HEL-1 proliferation |
| CN106333989A (en) * | 2016-11-19 | 2017-01-18 | 张忠远 | Pharmaceutical composition for treating wind-heat toothache |
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