CN103563849B - A kind of breeding method of high-yield black-skin chicken strain - Google Patents
A kind of breeding method of high-yield black-skin chicken strain Download PDFInfo
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Abstract
The invention discloses a kind of breeding method of high-yield black-skin chicken strain, belong to molecular breeding technology field.The present invention adopts the method qualification chicken crow skin loci gene type of molecule assisted Selection, can determine the genotype in this site fast, overcome the shortcomings such as test cross in traditional conventional breeding is loaded down with trivial details, the generation interval is long.Adopt method producer gene type of the present invention to be the high-yield black-skin chicken strain of FmFm, shorten the generation interval, accelerate breeding process, reduce cultivation cost; And this strain egg laying performance is good, egg nutrient is worth high; Eliminate hen crow skin proterties homogeneous, carcass is more attractive in appearance.In addition, this strain is used as male parent and high-yield egg chicken corss combination, the black skin chicken of more high productivity energy can be produced.
Description
Technical field
The present invention is specifically related to a kind of breeding method of high-yield black-skin chicken strain, belongs to molecular breeding technology field.
Background technology
Black-bone chicken is medicinal poultry well-known at home and abroad, within 1874, is admitted for standard variety in the world, and blackbone Chicken and black-bone chicken egg have special medicinal, nutrition and ornamental value.But the egg laying performance of black-bone chicken is low.And the egg laying performance of external high-yield egg chicken is very good.In order to improve its productivity, breeder often will import the blood relationship of high-yield egg chicken in the practices of breeding, and this will make black skin proterties be separated, and then the price of hen is eliminated in impact.In order to keep again the black skin proterties of superseded hen while providing productivity, the judgement of black skin loci gene type just seems extremely important.Usually adopt the method for test cross in traditional breeding method, the phenotypic character according to filial generation chicken is reserved seed for planting.Namely the black skin heterozygote individual in superseded Wu Fu chicken colony is carried out by test cross, Breeding and expanding population.But test cross process is comparatively loaded down with trivial details, and the cycle is long, and the impact of the size of group expanding can be subject to.
Summary of the invention
The object of this invention is to provide a kind of breeding method of high-yield black-skin chicken strain.
In order to realize above object, the technical solution adopted in the present invention is:
A breeding method for high-yield black-skin chicken strain, comprises the following steps:
(1) utilize black skin chicken breed to hybridize as female parent as male parent and high-yield egg chicken kind, produce F1 generation chicken;
(2) F1 generation selfing obtains F2 generation, and the F2 that selects and remain is for black skin chicken;
(3) quantitative fluorescent PCR is utilized to measure the Relative copy number of F2 for black skin chicken EDN3 gene, determine the genotype of F2 for black skin chicken individuals, black skin site of selecting and remain is the genotypic individuality of FmFm, and eliminating black skin site is the genotypic individuality of Fmfm, and obtaining black skin site is the genotypic male and female chicken of FmFm;
(4) traversed by carried out to the FmFm genotype individuals obtained and expand numerous, according to families selecting and individual choice method, improve egg production, purify simultaneously and fix black skin proterties, after seed selection more than 4 generations, obtaining black skin site is the genotypic high-yield black-skin chicken strain of FmFm.
Black skin chicken breed in described step (1) is Silky fowl, rivers and mountains black-bone chicken, Jinhu County's crow Salted chicken, Yugan black-bone chicken, Yun county sun black-bone chicken, snowy peak black-bone chicken, Sichuan Mountain Dark-bone Chickens, crow covers black-bone chicken, Tengchong snow cock, Lueyang chicken (Heihe black-bone chicken), he stays black-bone chicken, Wuliang Shan Mountain black-bone chicken, Yanjin black-bone chicken etc.
High-yield egg chicken kind in described step (1) is that Bai Laihang, Luo Man, Hai Lan, Hai Saikesi, Ni Ke, bar cloth cock, Hei Kang, Te Jia, snow Buddhist, Yi Sha, capital are red, capital powder, Hebei large noon, No. 3, agricultural university, new poplar are brown, new Yang Bai etc.
Quantitative fluorescent PCR is utilized to measure the Relative copy number of F2 for black skin chicken EDN3 gene in described step (3), determine that F2 is for the genotypic method of black skin chicken individuals, comprise the following steps: to comprise the chicken Whole Blood Genomic DNA to be measured of EDN3 gene for template, with primer pair P for primer carries out fluorescent quantitative PCR, the DNA sequence dna of amplified fragments is as shown in SEQIDNo.3;
Described primer pair P is:
Upstream primer P-F:5 '-CTTGACTTTTGGGATTTACCT-3 ' (as shown in SEQIDNo.1),
Downstream primer P-R:5 '-ACTGGTGCAGTATTTTCTGTT-3 ' (as shown in SEQIDNo.2).
The total length of this extension increasing sequence is 134bp, is positioned on the EDN3 gene of chicken crow skin site, can detect chicken crow skin gene loci EDN3 gene and whether there is copy number variation.
Described fluorescent quantitative PCR reaction system is: quantitative fluorescent PCR 2 × buffer solution 10 μ L, ddH
2o7 μ L, P-F0.5 μ L, P-R0.5 μ L, DNA profiling 2 μ L.
Described quantitative fluorescent PCR 2 × buffer solution mainly comprises 5U/ μ LTaqDNAPolymerase, 10 × Buffer, 25mMMgCl
2, 2mMdNTPs, SYBRGreenI.
Described fluorescent quantitative PCR response procedures is: 95 DEG C of denaturation 2min; 40 circulations: 95 DEG C of sex change 30s, 57 DEG C of annealing 30s, 72 DEG C extend 30s; 72 DEG C extend 10min; 20 DEG C of preservations.
Concrete, quantitative fluorescent PCR is utilized to measure the Relative copy number of F2 for black skin chicken EDN3 gene in described step (3), determine that F2 is for the genotypic method of black skin chicken individuals, further comprising the steps of: to utilize calibration curve to try to achieve the Relative copy number of chicken EDN3 gene to be measured, according to the genotype in its Relative copy number qualification chicken crow skin site.
The preparation method of described calibration curve is: using white skin chicken Whole Blood Genomic DNA as fluorescent quantitation standard items, concentration is respectively 80, and 40,20,10,5,2.5ng/ μ L, with primer pair P for primer carries out fluorescent quantitative PCR, with the Ct value of above-mentioned six standard items for ordinate, with the logarithm value of concentration for abscissa, Criterion curve, tries to achieve calibration curve equation.
The described method utilizing calibration curve to try to achieve the Relative copy number of chicken EDN3 gene to be measured is: before fluorescent quantitative PCR, first the DNA of chicken to be measured is diluted to same concentration 10ng/ μ L, again the Ct value of chicken to be measured is substituted in calibration curve equation, obtain the log concentration of its correspondence, be converted into concentration again, be the Relative copy number of this chicken EDN3 gene to be measured.
According to its Relative copy number, described identifies that the genotypic method in chicken crow skin site is: when the Relative copy number of chicken EDN3 gene to be measured is 2, this individuality is black skin homozygote (FmFm); When the Relative copy number of chicken EDN3 gene to be measured is 1.5, this individuality is black skin heterozygote (Fmfm); When the Relative copy number of chicken EDN3 gene to be measured is 1, this individuality is non-black skin homozygote (fmfm).
Preferably, black skin site step (4) obtained is that FmFm genotypic high yield type crow skin chicken is supporting as male parent and high-yield egg chicken inter breed crossing, produces the black skin chicken of more high productivity energy.
Beneficial effect of the present invention:
Adopt method producer gene type of the present invention to be the high-yield black-skin chicken strain of FmFm, not only avoid the loaded down with trivial details of test cross seed selection, shorten the generation interval, accelerate breeding process, reduce cultivation cost; And this strain egg laying performance is good, egg nutrient is worth high; Eliminate hen crow skin proterties homogeneous, carcass is more attractive in appearance.In addition, this strain is used as male parent and high-yield egg chicken corss combination, the black skin chicken of more high productivity energy can be produced.
The present invention adopts the method qualification chicken crow skin loci gene type of molecule assisted Selection, can determine the genotype in this site fast, shorten breeding time, improve breeding efficiency, overcome the shortcomings such as test cross in traditional conventional breeding is loaded down with trivial details, the generation interval is long.
Embodiment
Following embodiment is only described in further detail the present invention, but does not form any limitation of the invention.
Embodiment 1
The breeding method of high-yield black-skin chicken strain in the present embodiment, comprises the following steps:
(1) utilize Silky fowl be sheerly cock as male parent with carry out aircraft carrier chicken in vain and hybridize as female parent, produce F1 generation chicken;
(2) F1 generation selfing obtains F2 generation, and the F2 that selects and remain is for black skin chicken;
(3) utilize quantitative fluorescent PCR to measure F2 for the Relative copy number of black skin chicken EDN3 gene, determine the genotype of F2 for black skin chicken individuals, specifically comprise the following steps:
1. the collection of sample
To the black skin chicken wings venous blood collection that F2 generation selects and remain, add 1/3 anticoagulant, extract blood DNA with proteinase-K pathway;
2. DNA sample concentration adjustment
Utilize micro-spectrophotometer to carry out concentration determination the DNA of testing sample, according to surveyed concentration, each testing sample DNA concentration is added TE and is diluted to 10ng/ μ L, measure after dilution with micro-ultraviolet specrophotometer, 4 DEG C of Refrigerator stores are for subsequent use;
3. the preparation of standard items
Get the DNA sample of a white meat-type chickens at random, after measuring concentration, being diluted to 80ng/ μ L, as standard items mother liquor, is 80,40,20,10,5,2.5ng/ μ L by mother liquor doubling dilution, as 6 standard items of production standard curve;
4. design of primers
The sequence of the primer pair P used by genotype identification is as follows:
Upstream primer P-F:5 '-CTTGACTTTTGGGATTTACCT-3 ',
Downstream primer P-R:5 '-ACTGGTGCAGTATTTTCTGTT-3 ';
5. qPCR amplification
With primer pair P for amplimer, set up 20 μ L systems: quantitative fluorescent PCR 2 × buffer solution (comprises 5U/ μ LTaqDNAPolymerase, 10 × Buffer, 25mMMgCl
2, 2mMdNTPs, SYBRGreenI) and 10 μ L, ddH
2o7 μ L, P-F0.5 μ L, P-R0.5 μ L, DNA profiling 2 μ L; Response procedures is: 95 DEG C of denaturation 2min; 95 DEG C of sex change 30s, 57 DEG C of annealing 30s, 72 DEG C extend 30s, 40 circulations; 72 DEG C extend 10min; 20 DEG C of preservations;
6. the judgement of the process of qPCR amplification data and genotype results
With 80,40,20,10,5, the Ct value of 6 standard items of 2.5ng/ μ L for ordinate, with the logarithm of concentration for abscissa, Criterion curve, obtains the equation y=-0.285x+7.665 of calibration curve, R
2=0.9986 and the amplification efficiency 92.72% of this reaction;
Bring the Ct value of test individual into equation, obtain the log concentration of its correspondence, then be converted into concentration, be the Relative copy number of this test individual EDN3 gene;
The Relative copy number of the EDN3 gene of white skin individuality is set to 1, and when the Relative copy number of test individual is 2, the genotype in this site is FmFm; When the Relative copy number of test individual is 1.5, the genotype in this site is Fmfm(because sample to be tested DNA concentration calibration and quantitative fluorescent PCR test all can exist certain experimental error, by this control errors within 20%, do not affect and type is sentenced to black skin site);
(4) according to genotype result of determination, black skin site of selecting and remain is the genotypic individuality of FmFm, and eliminating black skin site is the genotypic individuality of Fmfm;
(5) traversed by carried out to the FmFm genotype individuals obtained and expand numerous, according to families selecting and individual choice method, improve egg production, purify simultaneously and fix black skin proterties, after seed selection more than 4 generations, obtaining black skin site is the genotypic high-yield black-skin chicken strain of FmFm;
(6) be that FmFm genotypic high yield type crow skin chicken is supporting as male parent and high-yield egg chicken inter breed crossing using the black skin site obtained, produce the black skin chicken of more high productivity energy.
Claims (9)
1. a breeding method for high-yield black-skin chicken strain, is characterized in that: comprise the following steps:
(1) utilize black skin chicken breed to hybridize as female parent as male parent and high-yield egg chicken kind, produce F1 generation chicken;
(2) F1 generation selfing obtains F2 generation, and the F2 that selects and remain is for black skin chicken;
(3) utilize quantitative fluorescent PCR to measure F2 for the Relative copy number of black skin chicken EDN3 gene, determine the genotype of F2 for black skin chicken individuals, black skin site of selecting and remain is the genotypic individuality of FmFm, and eliminating black skin site is the genotypic individuality of Fmfm;
(4) traversed by carried out to the FmFm genotype individuals obtained and expand numerous, according to families selecting and individual choice method, improve egg production, purify simultaneously and fix black skin proterties, after seed selection more than 4 generations, obtaining black skin site is the genotypic high-yield black-skin chicken strain of FmFm;
Quantitative fluorescent PCR is utilized to measure the Relative copy number of F2 for black skin chicken EDN3 gene in described step (3), determine that F2 is for the genotypic method of black skin chicken individuals, comprise the following steps: to comprise the chicken Whole Blood Genomic DNA to be measured of EDN3 gene for template, with primer pair P for primer carries out fluorescent quantitative PCR, the DNA sequence dna of amplified fragments is as shown in SEQIDNo.3;
Described primer pair P is:
Upstream primer P-F:5 '-CTTGACTTTTGGGATTTACCT-3 ',
Downstream primer P-R:5 '-ACTGGTGCAGTATTTTCTGTT-3 '.
2. the breeding method of high-yield black-skin chicken strain according to claim 1, is characterized in that: the black skin chicken breed in described step (1) is Silky fowl, rivers and mountains black-bone chicken, Jinhu County's crow Salted chicken, Yugan black-bone chicken, Yun county sun black-bone chicken, snowy peak black-bone chicken, Sichuan Mountain Dark-bone Chickens, crow covers black-bone chicken, Tengchong snow cock, Lueyang chicken, he stays black-bone chicken, Wuliang Shan Mountain black-bone chicken or Yanjin black-bone chicken.
3. the breeding method of high-yield black-skin chicken strain according to claim 1, is characterized in that: the high-yield egg chicken kind in described step (1) is that Bai Laihang, Luo Man, Hai Lan, Hai Saikesi, Ni Ke, bar cloth cock, Hei Kang, Te Jia, snow Buddhist, Yi Sha, capital are red, capital powder, Hebei large noon, No. 3, agricultural university, new poplar are brown or new Yang Bai.
4. the breeding method of high-yield black-skin chicken strain according to claim 1, is characterized in that: described fluorescent quantitative PCR reaction system is: quantitative fluorescent PCR 2 × buffer solution 10 μ L, ddH
2o7 μ L, P-F0.5 μ L, P-R0.5 μ L, DNA profiling 2 μ L.
5. the breeding method of high-yield black-skin chicken strain according to claim 1, is characterized in that: described fluorescent quantitative PCR response procedures is: 95 DEG C of denaturation 2min; 40 circulations: 95 DEG C of sex change 30s, 57 DEG C of annealing 30s, 72 DEG C extend 30s; 72 DEG C extend 10min; 20 DEG C of preservations.
6. the breeding method of high-yield black-skin chicken strain according to claim 1, it is characterized in that: in described step (3), utilize quantitative fluorescent PCR to measure the Relative copy number of F2 for black skin chicken EDN3 gene, determine that F2 is for the genotypic method of black skin chicken individuals, further comprising the steps of: to utilize calibration curve to try to achieve the Relative copy number of chicken EDN3 gene to be measured, according to the genotype in its Relative copy number qualification chicken crow skin site.
7. the breeding method of high-yield black-skin chicken strain according to claim 6, it is characterized in that: the described method utilizing calibration curve to try to achieve the Relative copy number of chicken EDN3 gene to be measured is: before fluorescent quantitative PCR, first the DNA of chicken to be measured is diluted to same concentration 10ng/ μ L, the Ct value of chicken to be measured is substituted in calibration curve equation, obtain the log concentration of its correspondence, be converted into concentration again, be the Relative copy number of this chicken EDN3 gene to be measured.
8. the breeding method of high-yield black-skin chicken strain according to claim 6, it is characterized in that: according to its Relative copy number, described identifies that the genotypic method in chicken crow skin site is: when the Relative copy number of chicken EDN3 gene to be measured is 2, this individuality is black skin homozygote; When the Relative copy number of chicken EDN3 gene to be measured is 1.5, this individuality is black skin heterozygote.
9. the breeding method of high-yield black-skin chicken strain according to claim 1, it is characterized in that: black skin site step (4) obtained is that FmFm genotypic high yield type crow skin chicken is supporting as male parent and high-yield egg chicken inter breed crossing, produces the black skin chicken of more high productivity energy.
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| CN104161017B (en) * | 2014-08-14 | 2016-05-11 | 兴文县新星农业发展有限责任公司 | The breeding method of a kind of black-bone chicken |
| CN104521882A (en) * | 2014-12-12 | 2015-04-22 | 安徽荣达禽业开发有限公司 | Five-toed black-speckle feather laying hen breeding method |
| CN104642265B (en) * | 2015-02-05 | 2017-08-25 | 河南农业大学 | A kind of Fast-Balance breeding method of high-quality chicken breed |
| CN106070044A (en) * | 2016-06-29 | 2016-11-09 | 来宾市忻城县润华牧业有限责任公司 | A kind of reduction is raised in cages kind by the breeding method of common chicken wild nature |
| CN106172195B (en) * | 2016-07-19 | 2018-09-25 | 贵州省普安县万象生态农业综合开发有限公司 | A method of improving Pu'an seedling mountain black-bone chicken laying rate |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1465238A (en) * | 2002-06-11 | 2004-01-07 | 康相涛 | Method for breeding new strain chicken with yellow (white) tibiala and complete set application |
| CN1623380A (en) * | 2003-12-03 | 2005-06-08 | 康相涛 | Method of raising black-bone chicken |
| CN101990857A (en) * | 2009-08-18 | 2011-03-30 | 江苏省家禽科学研究所 | Method for breeding fast-growing silkie chicken line |
| CN102144614A (en) * | 2011-04-29 | 2011-08-10 | 赵河山 | Method for breeding new strain of black-bone chicken with higher production performance |
| CN103120143A (en) * | 2013-01-23 | 2013-05-29 | 河南农业大学 | Cultivating method of laying hen with low egg yolk cholesterol content |
-
2013
- 2013-10-24 CN CN201310507256.7A patent/CN103563849B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1465238A (en) * | 2002-06-11 | 2004-01-07 | 康相涛 | Method for breeding new strain chicken with yellow (white) tibiala and complete set application |
| CN1623380A (en) * | 2003-12-03 | 2005-06-08 | 康相涛 | Method of raising black-bone chicken |
| CN101990857A (en) * | 2009-08-18 | 2011-03-30 | 江苏省家禽科学研究所 | Method for breeding fast-growing silkie chicken line |
| CN102144614A (en) * | 2011-04-29 | 2011-08-10 | 赵河山 | Method for breeding new strain of black-bone chicken with higher production performance |
| CN103120143A (en) * | 2013-01-23 | 2013-05-29 | 河南农业大学 | Cultivating method of laying hen with low egg yolk cholesterol content |
Non-Patent Citations (3)
| Title |
|---|
| A Complex Genomic Rearrangement Involving the Endothelin 3 Locus Causes Dermal Hyperpigmentation in the Chicken;Ben Dorshorst et al;《PLOS GENETICS》;20111231;第7卷(第12期);第2页右栏倒数第1段，第4页右栏倒数第1段，图1-6 * |
| 乌蒙乌骨鸡分别与肉鸡蛋鸡杂交试验研究;李未博等;《当代畜牧》;20110125(第1期);第31-32页 * |
| 金湖乌骨鸡肤色遗传观测;贺生中等;《安徽农业科学》;20081020(第30期);第13167-13168页 * |
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