CN103558371A - Test paper for detecting carbon dioxide and preparation method of test paper - Google Patents
Test paper for detecting carbon dioxide and preparation method of test paper Download PDFInfo
- Publication number
- CN103558371A CN103558371A CN201310525050.7A CN201310525050A CN103558371A CN 103558371 A CN103558371 A CN 103558371A CN 201310525050 A CN201310525050 A CN 201310525050A CN 103558371 A CN103558371 A CN 103558371A
- Authority
- CN
- China
- Prior art keywords
- solution
- carbon dioxide
- test paper
- paper
- gas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/521—Single-layer analytical elements
- G01N33/523—Single-layer analytical elements the element being adapted for a specific analyte
Abstract
The invention belongs to the field of clinical medicine and environmental health monitoring and particularly relates to test paper for detecting carbon dioxide and a preparation method of the test paper. The test paper is prepared by using a following method comprising the steps: A, dissolving ethyecellulose in acetone and agitating; adding bromothymol blue, bromcresol purple, 2-ethyl hexyloxy propylamine, sodium sulfafe decahydrate, polyethylene glycol 600, distilled water, calcium carbonate and silicon dioxide; agitating and mixing uniformly so as to obtain a solution; putting the solution into a closed container and standing for a period of time at a certain temperature condition; B, namely preparing a sodium hydroxide solution and putting a piece of paper with the good water-absorbing quality into the solution to be immersed sufficiently; then drying the paper in a drying oven; C, adding a certain amount of the distilled water in the solution obtained in the step A until the solution becomes bluish violet; dripping the solution to the paper treated in the step B and putting the paper into the drying oven to the dried. The test paper is convenient to use, low in construction cost and sensitive in detection, and can be widely applied to the field of the clinical medicine and the environmental health monitoring.
Description
Technical field
The invention belongs to clinical medicine and environmental health monitoring and surveilliance field, relate to more specifically a kind of carbon dioxide and detect with test paper and preparation method thereof.
Background technology
In clinical medicine and environmental health monitoring and surveilliance field, often need the concentration of carbon dioxide in gas-monitoring.For example, in clinical operation, fast, accurately judge that tracheal catheter position is very important in first aid, recovery and anaesthesia process, as incorrect in catheter position or be strayed into esophagus and do not find at short notice this mistake, just likely to patient, cause serious harm even dead, the most effective current discrimination method is to detect carbon dioxide in expiratory air, in most cases, is all to detect by instrument, yet instrument cost is high, carry and use equal inconvenience.External also have take chemical stain method and detect the method for the gas concentration lwevel in expiratory air as means, but the contrast of its indicator discoloration is little, is difficult for observing.
Summary of the invention
The object of the invention is to overcome above-mentioned deficiency, provide a kind of easy to use, cheap, detect sensitive carbon dioxide detection test paper.
The technical solution adopted for the present invention to solve the technical problems is:
1, carbon dioxide detects the preparation method with test paper, it is characterized in that: comprise the steps:
A: ethyl cellulose is dissolved in acetone, stirs, this solution is fully mixed; Under the condition constantly stirring, add wherein bromthymol blue, bromcresol purple, 2-ethyl hexyl oxy propylamine, sal glauberi, Macrogol 600, distilled water, calcium carbonate, silicon dioxide, be uniformly mixed, then solution is placed in airtight container to standing a period of time under certain temperature conditions;
B: preparation sodium hydroxide solution, the paper of good water absorption is put into this solution and fully infiltrate, and then dry in baking oven;
C: get the solution that steps A obtains, add wherein a certain amount of distilled water, till making solution be bluish violet, then fluid drips is now added in above the paper that B step handled well, then put into baking oven and dry.
Preferably, in steps A, the consumption of each material is: ethyl cellulose 1～4g, acetone 15～60ml, bromthymol blue 0.1～0.4g, bromcresol purple 0.05～0.2g, 2-ethyl hexyl oxy propylamine 0.1～0.4ml, sal glauberi 0.2～0.8g, Macrogol 6000 .2～0.8ml, distilled water 0.2～0.9ml, calcium carbonate 0.2～0.9g, silicon dioxide 0.2～0.8g; In step B, concentration of sodium hydroxide solution is 0.05-0.3mol/L.
Carbon dioxide detection of the present invention is 0% to 5% by the concentration range that test paper can detect gaseous carbon dioxide, preferably from 1%-5%.When having carbon dioxide to exist, test paper of the present invention can instantaneous variable color, and after carbon dioxide removes, test paper is by momentary recovery primary colors.And along with the rising (from 0% → 5%) of gas concentration lwevel, the color of test paper also can be from bottle green → light green color → yellow green → lemon yellow.
Applicant's surprised discovery when above-mentioned preparation method is further optimized, in step (A), dwell temperature and time of repose can produce important impact to the sensitivity of final test paper, applicant has further obtained the suitableeest temperature and rest time by series of experiments, to obtain best sensitivity.
Preferably, in preparation method of the present invention, in steps A, standing temperature is 20 ℃-60 ℃, more preferably 30 ℃-40 ℃, and more preferably 33 ℃.
Preferably, in preparation method of the present invention, in steps A, time of repose is 5min-30min, more preferably 5min-10min, more preferably 8min.
Another technical scheme of the present invention is:
The carbon dioxide detection test paper that method of the present invention prepares.
Another technical scheme of the present invention is:
Carbon dioxide of the present invention detects the purposes with test paper: it is characterized in that: for detection of the gas that contains low concentration of carbon dioxide.
Preferably, carbon dioxide in gas concentration is 0-5%, is preferably 1%-5%.
Preferably, the gas that the gas of detection is behaved and breathed out.
The purposes of test paper for carbon dioxide of the present invention detects: it is characterized in that: the detection of the gas that contains low concentration of carbon dioxide for clinical medicine and environmental health monitoring and surveilliance field.
The invention provides a kind of easy to use, cheap, detect sensitive carbon dioxide detection test paper, applicant has carried out further optimization to its preparation method simultaneously, has obtained the suitableeest every parameter in preparation process.Carbon dioxide of the present invention detects can be widely used in clinical medicine and environmental health monitoring and surveilliance field with test paper.
Embodiment
Embodiment 1 carbon dioxide detects with the preparation of test paper and the screening of parameter
Experiment 1: prepare according to the following steps carbon dioxide detection test paper 1-20:
A: 1g ethyl cellulose is dissolved in 15mL acetone, and high-speed stirred 1h, fully mixes this solution; Under the condition constantly stirring, add wherein the 2-ethyl hexyl oxy propylamine (while just having added, yellow just becomes purple), the sal glauberi of 0.2g, 0.2mL Macrogol 600, the distilled water of 0.2ml of 0.1g bromthymol blue, 0.05g bromcresol purple (now the color of solution is for yellow), 0.1mL, the silicon dioxide of the calcium carbonate of 0.2g, 0.2g, stir 1h (object: ground and mixed is even), then solution is placed in airtight container to standing a period of time under certain temperature conditions.
B: the sodium hydroxide solution of preparation 0.05mol/L, the paper of good water absorption is put into this solution 30min, and then dry in baking oven.
C: get the solution that steps A obtains, add a certain amount of distilled water in the solution taking out, making solution is (A solution: distilled water volume ratio is approximately 1:4) till bluish violet.Then fluid drips is now added in above the paper that B step handled well, then puts into baking oven and dry.
Temperature and time when wherein standing in steps A is chosen respectively 20 groups of listed different values of table 1, thereby obtain respectively carbon dioxide, detects and uses test paper 1-20.
| Group | Dwell temperature | Time of repose |
| 1 | 20℃ | 5min |
| 2 | 20℃ | 8min |
| 3 | 20℃ | 10min |
| 4 | 20℃ | 30mn |
| 5 | 30℃ | 5min |
| 6 | 30℃ | 8min |
| 7 | 30℃ | 10min |
| 8 | 30℃ | 30mn |
| 9 | 33℃ | 5min |
| 10 | 33℃ | 8min |
| 11 | 33℃ | 10min |
| 12 | 33℃ | 30mn |
| 13 | 40℃ | 5min |
| 14 | 40℃ | 8min |
| 15 | 40℃ | 10min |
| 16 | 40℃ | 30mn |
| 17 | 60℃ | 5min |
| 18 | 60℃ | 8min |
| 19 | 60℃ | 10min |
| 20 | 60℃ | 30mn |
Experiment 2: prepare according to the following steps carbon dioxide detection test paper 21-40:
A: 4g ethyl cellulose is dissolved in 60mL acetone, and high-speed stirred 1h, fully mixes this solution; Under the condition constantly stirring, add wherein the 2-ethyl hexyl oxy propylamine (while just having added, yellow just becomes purple), the sal glauberi of 0.8g, 0.8mL Macrogol 600, the distilled water of 0.9ml of 0.4g bromthymol blue, 0.2g bromcresol purple (now the color of solution is for yellow), 0.4mL, the silicon dioxide of the calcium carbonate of 0.9g, 0.8g, stir 1h (object: ground and mixed is even), then solution is placed in airtight container to standing a period of time under certain temperature conditions.
B: the sodium hydroxide solution of preparation 0.3mol/L, the paper of good water absorption is put into this solution 30min, and then dry in baking oven.
C: get the solution that steps A obtains, add a certain amount of distilled water in the solution taking out, making solution is (A solution: distilled water volume ratio is approximately 1:4) till bluish violet.Then fluid drips is now added in above the paper that B step handled well, then puts into baking oven and dry.
Temperature and time when wherein standing in steps A is chosen respectively 20 groups of listed different values of table 1, thereby obtain respectively carbon dioxide, detects and uses test paper 21-40.
Embodiment 2 carbon dioxide detect with test paper sensitivity and detect
The carbon dioxide that embodiment 1 is prepared detects and puts into respectively closed container with test paper 1-40, to passing into concentration in container, be 5% carbon dioxide (gas componant and people exhale composition identical), detect its variable color (becoming lemon yellow from bottle green) required time, result is as shown in the table:
Table 2
By above-mentioned experimental data, can be found out, in the sensitivity of test paper and steps A, dwell temperature and time of repose have very large relation.In steps A, dwell temperature is in the time of 30 ℃-40 ℃, and test paper has better sensitivity, and when time of repose is 5min-10min, test paper has better sensitivity, and work as temperature, is 33 ℃, and when time of repose is 8min, test paper has the highest sensitivity.
Claims (10)
1. a carbon dioxide detection test paper, is characterized in that: it can detect the gas that contains low concentration of carbon dioxide.
2. carbon dioxide detects the preparation method with test paper, it is characterized in that: comprise the steps:
A: ethyl cellulose is dissolved in acetone, stirs, this solution is fully mixed; Under the condition constantly stirring, add wherein bromthymol blue, bromcresol purple, 2-ethyl hexyl oxy propylamine, sal glauberi, Macrogol 600, distilled water, calcium carbonate, silicon dioxide, be uniformly mixed, then solution is placed in airtight container to standing a period of time under certain temperature conditions;
B: preparation sodium hydroxide solution, the paper of good water absorption is put into this solution and fully infiltrate, and then dry in baking oven;
C: get the solution that steps A obtains, add wherein a certain amount of distilled water, till making solution be bluish violet, then fluid drips is now added in above the paper that B step handled well, then put into baking oven and dry.
3. method as claimed in claim 2, it is characterized in that: in steps A, the consumption of each material is: ethyl cellulose 1～4g, acetone 15～60ml, bromthymol blue 0.1～0.4g, bromcresol purple 0.05～0.2g, 2-ethyl hexyl oxy propylamine 0.1～0.4ml, sal glauberi 0.2～0.8g, Macrogol 6000 .2～0.8ml, distilled water 0.2～0.9ml, calcium carbonate 0.2～0.9g, silicon dioxide 0.2～0.8g; In step B, concentration of sodium hydroxide solution is 0.05～0.3mol/L.
4. the method as described in claim 2-3 any one, is characterized in that: temperature when standing in steps A is that temperature is 20 ℃-60 ℃, is preferably 30 ℃-40 ℃, more preferably 33 ℃.
5. the method as described in claim 2-4 any one, is characterized in that: in steps A, the standing time is 5min-30min, more preferably 5min-10min, more preferably 8min.
6. the carbon dioxide detection test paper that claim 2-5 any one method is prepared from.
7. described in claim 6, carbon dioxide detects the purposes with test paper, it is characterized in that: for detection of the gas that contains low concentration of carbon dioxide.
8. purposes as claimed in claim 7, is characterized in that: carbon dioxide in gas concentration is 0-5%, is preferably 1%-5%.
9. purposes as claimed in claim 7, is characterized in that: the gas that the gas of detection is behaved and breathed out.
10. described in claim 6, carbon dioxide detects the purposes with test paper, it is characterized in that: the detection of the gas that contains low concentration of carbon dioxide for clinical medicine and environmental health monitoring and surveilliance field.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310525050.7A CN103558371A (en) | 2013-10-29 | 2013-10-29 | Test paper for detecting carbon dioxide and preparation method of test paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310525050.7A CN103558371A (en) | 2013-10-29 | 2013-10-29 | Test paper for detecting carbon dioxide and preparation method of test paper |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103558371A true CN103558371A (en) | 2014-02-05 |
Family
ID=50012677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310525050.7A Pending CN103558371A (en) | 2013-10-29 | 2013-10-29 | Test paper for detecting carbon dioxide and preparation method of test paper |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103558371A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110412239A (en) * | 2018-04-27 | 2019-11-05 | 大连理工大学 | A kind of quickly detection Microbial biomass C O2The gel test paper and its application method of respiratory rate |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3884641A (en) * | 1972-10-11 | 1975-05-20 | Merck Patent Gmbh | Reaction chamber unit for test strips |
| CN87105619A (en) * | 1986-08-13 | 1988-02-24 | 卡尔·格·费德尔 | Device indicating |
| CN1114748A (en) * | 1994-12-03 | 1996-01-10 | 中国人民解放军第四七八医院 | Carbon dioxide indicator and preparation process |
| CN1183553A (en) * | 1997-11-17 | 1998-06-03 | 中国电影资料馆 | Gas-sensitive pH value test paper |
| CN1240026A (en) * | 1996-12-09 | 1999-12-29 | 内斯托尔体系Ab | Device for trapping and assaying carbon and method of use |
-
2013
- 2013-10-29 CN CN201310525050.7A patent/CN103558371A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3884641A (en) * | 1972-10-11 | 1975-05-20 | Merck Patent Gmbh | Reaction chamber unit for test strips |
| CN87105619A (en) * | 1986-08-13 | 1988-02-24 | 卡尔·格·费德尔 | Device indicating |
| CN1114748A (en) * | 1994-12-03 | 1996-01-10 | 中国人民解放军第四七八医院 | Carbon dioxide indicator and preparation process |
| CN1240026A (en) * | 1996-12-09 | 1999-12-29 | 内斯托尔体系Ab | Device for trapping and assaying carbon and method of use |
| CN1183553A (en) * | 1997-11-17 | 1998-06-03 | 中国电影资料馆 | Gas-sensitive pH value test paper |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110412239A (en) * | 2018-04-27 | 2019-11-05 | 大连理工大学 | A kind of quickly detection Microbial biomass C O2The gel test paper and its application method of respiratory rate |
| CN110412239B (en) * | 2018-04-27 | 2021-08-10 | 大连理工大学 | Rapid detection of microbial CO2Gel test paper for respiratory rate and use method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107367470A (en) | A kind of method of Judgment of Determination of Total Phosphorus In Water With Ammonium Molybdate Spectrophotometry | |
| CN109342385B (en) | Carbon quantum dot for rapidly detecting nitrite content in food and environment and application method thereof | |
| CN109731454A (en) | Visualize formaldehyde scavenger and preparation method thereof | |
| CN113416799B (en) | CDA primer group and kit for detecting African swine fever virus and application of CDA primer group and kit | |
| CN105021601A (en) | Food sulfur dioxide content determination method | |
| CN107421932B (en) | Utilize the method for nitrogen phosphorus doping carbon quantum dot probe in detecting Cr VI | |
| CN101592624A (en) | Be applicable to the microcalorimetric method of microbial count situation in the fast detecting food | |
| CN106706528A (en) | Aptamer-based tetracycline colorimetric detection method | |
| CN103630534A (en) | Quick detection method for sulfur dioxide in food | |
| CN107880034A (en) | A kind of fluorescence probe of Visual retrieval hydrazine based on benzothiazole and its production and use | |
| CN105503965A (en) | Rare earth (III)-transition (II) mixed metal fluorescence complex and preparing method and application thereof | |
| CN104531885B (en) | Aeromonas veronii quick detection primer, kit and application | |
| CN103558371A (en) | Test paper for detecting carbon dioxide and preparation method of test paper | |
| CN115389476A (en) | Fluorescent label for nondestructive visual monitoring of freshness of fish and meat products and preparation method thereof | |
| CN104034711A (en) | Method for detecting potassium dichromate by utilizing graphene quantum dot probe | |
| CN109652597A (en) | It is a kind of for detect dog, cat, ermine parvovirus general RPA primer, RPA probe, kit and nucleic acid detection method | |
| CN105806839A (en) | Embryo-free rice freshness detecting reagent and method | |
| CN111518554A (en) | Copper-doped carbon quantum dot with mimic enzyme property and preparation method and application thereof | |
| CN106966962A (en) | A kind of probe and its preparation method and application | |
| CN103543148A (en) | Carbon dioxide detector | |
| CN110218775A (en) | PCR fluorescence visual colorimetric determination detection method and kit based on ruthenium complex | |
| CN109342341A (en) | A method of utilizing carbon quantum dot Visual retrieval concentration of hydrogen peroxide | |
| CN110144381A (en) | Coliform zymolyte method measurement agent and preparation method thereof in water | |
| CN101481727A (en) | Pressure steam sterilizing effect indicator and use thereof | |
| CN111763330B (en) | Chain-structured rare earth europium (III) coordination polymer and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20150401 Address after: 225300 Jiangsu city of Taizhou province China pharmaceutical Road East, Xinyang City Road on the north side (G34 building) Applicant after: Jiangsu Deweilan Medical Instrument Co., Ltd. Applicant after: Zhou Xiao Address before: 225300 Jiangsu city of Taizhou province China pharmaceutical Road East, Xinyang City Road on the north side (G34 building) Applicant before: Jiangsu Deweilan Medical Instrument Co., Ltd. Applicant before: Liu Bozhi Applicant before: Zhou Xiao |
|
| AD01 | Patent right deemed abandoned |
Effective date of abandoning: 20160601 |
|
| C20 | Patent right or utility model deemed to be abandoned or is abandoned |