CN103555791A - Clustering method for inspecting influence of slow viruses containing carp IGF2b (Insulin Growth Factor 2b) gene on physiques of carps - Google Patents
Clustering method for inspecting influence of slow viruses containing carp IGF2b (Insulin Growth Factor 2b) gene on physiques of carps Download PDFInfo
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- CN103555791A CN103555791A CN201310516509.7A CN201310516509A CN103555791A CN 103555791 A CN103555791 A CN 103555791A CN 201310516509 A CN201310516509 A CN 201310516509A CN 103555791 A CN103555791 A CN 103555791A
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Abstract
The invention provides a clustering method for inspecting the influence of slow viruses containing carp IGF2b (Insulin Growth Factor 2b) gene on the physiques of carps, which belongs to the technical field of fish culture. The clustering method comprises the following steps of: step 1, injecting slow viruses containing carp IGF2b gene and with different concentrations in different groups of carp samples, and culturing; and inspecting the physique indexes and weight indexes of the cultured carps, finding the physique indexes with remarkable influence on the weight indexes, clustering by taking the selected physique indexes as characteristic values, and inspecting the influence of different injection amounts on the physiques. The method is capable of avoiding the interference of the weight indexes on physique parameters, thus judging the influence of the injection amounts of the slow viruses containing carp IGF2b gene on the physique indexes of the carps, and capable of solving the problem of mutual interference of a plurality of indexes among the test groups with different injection amounts.
Description
Technical field
The invention provides a kind ofly for investigating the clustering method that affects carp physique containing carp IGF2b gene slow virus, belong to fishery cultivating technical field.
Background technology
In scientific research and testing and technological development, several treatment group are usually set, be used for determining main test factor of influence, the dosage of factor of influence and influence degree etc.Often not only one of the index of processing in the test of this class, in a trophic factor test, testing index has the index of the many aspects such as physiochemical indice, genetic expression figureofmerit, growth traits index, amynologic index.Yet, for the data processing of this respect mainly by variance analysis and regression analysis, and the combined influence to these indexs between how to confirm different treatment group, rarely seen report.
Take carp culture as example, Chinese patent 201210087621.9 discloses the method that slow virus that a kind of utilization contains carp IGF2b gene is improved carp meat yield, the method result shows: with the slow virus infection carp young that contains carp IGF2b gene having detected, after 3 months, infected group is compared control group, carp body weight has obtained remarkable increase, not impact of carp blood indices simultaneously, and security is good.
But because the growth indexes of carp not only has body weight, also have the thick etc. such as body length, height, body of other, these physique indexs also have important value in actual procedure, but these indexs can be subject to the impact of body weight again when measuring, and cause adopting the analytical procedures such as conventional classification, recurrence cannot accurately investigate slow virus injection rate for the impact of physique parameter.
Summary of the invention
The object of the invention is: provide a kind of and can avoid the interference of body weight index and can judge the method for slow virus injection rate to carp physique Index Influence that contains carp IGF2b gene, and can solve the problem of a plurality of index phase mutual interference between the test group of different injection rates, the method can be for reaching the object of the different physique carp samples of screening by adjusting slow virus injection volume.Technical scheme is as follows:
Investigation affects a clustering method for carp physique containing carp IGF2b gene slow virus, it is characterized in that, comprises the steps:
The first step, preparation carp sample, grouping; The slow virus that contains carp IGF2b gene of injecting different concns in the carp sample body of different sample groups, cultivation;
Second step, the carp sample after cultivation is measured to following 7 indexs: body weight, body length, height, body is thick, fish tail handle is long, caudal peduncle is thick, the front distance of ridge;
The 3rd step, take body weight as dependent variable, take body length, height, body is thick, fish tail handle is long, caudal peduncle is thick, before ridge apart from being parameter, carry out regression analysis, find the physique index that body weight is had a significant impact;
The physique index that body weight is had a significant impact that the 4th step, the 3rd step of usining obtain is as eigenwert, different sample groups are carried out to cluster analysis, according to the sample of various dose group, by cluster, to inhomogeneous result, investigated the impact for physique parameter containing carp IGF2b gene slow virus.
In the present invention, before described ridge, distance refers to the distance of carp rhynchodaenm to the first ridge; Described physique index refers to body length, height, body is thick, fish tail handle is long, caudal peduncle is thick, the front distance of ridge.
As of the present invention preferred, the physique index that body weight is had a significant impact described in the 3rd step refers to that body is long, body is thick and caudal peduncle is long.
As of the present invention preferred, the regression analysis described in the 3rd step refers to linear regression.
As of the present invention preferred, in the regression analysis described in the 3rd step, determine whether that the standard that tool has a significant impact is: in correlation analysis, when P≤0.05, think that tool has a significant impact.
The principle that realizes of the present invention: injecting lentivirus can produce significantly impact to body weight in carp sample, in addition again because physique index can be larger with body weight dependency, and body weight and body length, height, body are thick etc. collinearity problem, therefore in conventional analytical procedure, cannot measure in various dose group injected dose for the impact of physique; The present invention is by finding the index having a significant effect for body weight, utilize these indexs that filter out as eigenwert, the impact that can avoid body weight to judge for physique result, next to the physique index of these screenings as eigenwert, whether the sample for different injected dose carries out cluster analysis, just can obtain injected dose and can exert an influence for physique.
Accompanying drawing explanation
Fig. 1 is the signal of distance measurement method before ridge.
Fig. 2 is the result schematic diagram of carrying out cluster analysis in the 2nd step of embodiment.
Fig. 3 is the result schematic diagram of carrying out cluster analysis in the 4th step of embodiment.
Embodiment
1. carp sample is divided into 5 groups at random, every group of 200 tails, the method that is 201210087621.9 according to the patent No. contains the injection of carp IGF2b gene slow virus, negative control is left intact, positive control is made injection treatment, and the injected dose of 3 treatment group is respectively 10 μ L, 20 μ L, 40 μ L; Cultivate after 5 months again, carry out DATA REASONING for the second time, measurement index is that body weight, body length, height, body are thick, increases to measure that fish tail handle is long, caudal peduncle is thick, and measures first the distance (before called after ridge apart from) of carp rhynchodaenm to the first ridge, sees Fig. 1.
2. a), by the ward method of software R 2.14.0,7 of all treatment group indexs are carried out to cluster as eigenwert, dendrogram is shown in A part in Fig. 2; B), again this index of body weight is deleted, by 6 remaining physique indexs, as eigenwert, carry out cluster analysis, dendrogram is shown in B part in Fig. 2, can find out, after body weight index is deleted, the result of cluster does not change, this is mainly the problem of collinearity that has between a plurality of indexs due to body weight and physique, and some physique indexs are larger on the impact of body weight, has caused retaining or to delete body weight index little for the result impact of cluster.C) according to known, due to body weight and body long, height, there is collinearity problem in the thick grade of body, therefore, in order to solve that body weight and physique index exist relation mutually and the problem that cannot classify, by 6 physique indexs respectively divided by body weight (be about to 6 physique standard parameters), can avoid like this repetition causing due to collinearity, also the relation of the two can be taken into account simultaneously, these 6 standardized physique parameters are carried out to cluster analysis as eigenwert, as shown in C part in Fig. 2, can find out, there is variation in the result of cluster, in B part, be that 10 μ L and 40 μ L first gather it is one group, and be that 10 μ L and 20 μ L first gather in C part, it is one group, whether the standardization that body weight is described has had influence on the treatment effect assessment between different treatment group, whether the stdn of body weight, the Cluster Assessment that obtains treatment effect is different, that is to say that the result in this step can not really reflect treatment effect definite between different treatment group.
3. next, in order to overcome body weight for the impact of physique index, need to find the index on remarkably influenced body weight, what adopt is the method for multiple stepwise regression, by soft SA S8.0, make the multiple stepwise regression equation of 3 treatment group, 6 indexs have all been carried out multiple stepwise regression, but only have 3 factors to reach significance level, respectively: body is long, body is thick and caudal peduncle is long, other 3 indexs do not have significance (criterion of significance is P≤0.05).Regression equation result is as follows:
Y=-50.07+0.64x
1+1.35x
2-0.55x
3 （1）
Y=-39.41+0.31x
1+1.94x
2 （2）
Y=-62.46+0.65x
1+1.37x
2 （3）
In formula, (1) formula means 10 μ L treatment group; (2) formula means 20 μ L treatment group; (3) formula means 40 μ L treatment group.Y represents body weight, x
1represent that body is long, x
2represent that body is thick, x
3represent that caudal peduncle is long.
4. last, by R software 2.14.0 is long to the index body of screening in (3), body is thick and the cluster analysis of caudal peduncle progress row, A and the B part of seeing Fig. 3.Result shows, no matter whether stdn of body weight, all be difficult to change the result of cluster, in this example, it is a class that 10 μ L and 20 μ L treatment group are first gathered, and then and 40 μ L treatment group to gather be a class, contain IGF2b slow virus and process carp, along with the increase of concentration for the treatment of, when 40 μ L process, there is change in build.
Comprehensive above step, we can obtain, this method is processed carp test from containing IGF2b slow virus, influence index has been carried out to cluster, and by the relation between leading indicator and non-leading indicator, found out the deficiency existing in cluster analysis, and variance analysis, not detectable problem in correlation analysis, then by setting up multiple stepwise regression variance, determined the Significance factors that affects body weight, by the cluster of these factors, the treatment effect of different treatment group and the association between them have finally been determined, this just can avoid the interference of body weight and remove to investigate slow virus injection volume for the impact of body build index.
Claims (4)
1. investigation affects a clustering method for carp physique containing carp IGF2b gene slow virus, it is characterized in that, comprises the steps:
The first step, preparation carp sample, grouping; The slow virus that contains carp IGF2b gene of injecting different concns in the carp sample body of different sample groups, cultivation;
Second step, the carp sample after cultivation is measured to following 7 indexs: body weight, body length, height, body is thick, fish tail handle is long, caudal peduncle is thick, the front distance of ridge;
The 3rd step, take body weight as dependent variable, take body length, height, body is thick, fish tail handle is long, caudal peduncle is thick, before ridge apart from being parameter, carry out regression analysis, find the physique index that body weight is had a significant impact;
The physique index that body weight is had a significant impact that the 4th step, the 3rd step of usining obtain is as eigenwert, different sample groups are carried out to cluster analysis, according to the sample of various dose group, by cluster, to inhomogeneous result, investigated the impact for physique parameter containing carp IGF2b gene slow virus.
2. investigation according to claim 1 affects the clustering method of carp physique containing carp IGF2b gene slow virus, it is characterized in that: the physique index that body weight is had a significant impact described in the 3rd step refers to that body is long, body is thick and caudal peduncle is long.
3. investigation according to claim 1 affects the clustering method of carp physique containing carp IGF2b gene slow virus, it is characterized in that: the regression analysis described in the 3rd step refers to linear regression.
4. investigation according to claim 1 affects the clustering method of carp physique containing carp IGF2b gene slow virus, it is characterized in that: in the regression analysis described in the 3rd step, determine whether that the standard that tool has a significant impact is: in correlation analysis, when P≤0.05, think that tool has a significant impact.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250912A (en) * | 2011-06-10 | 2011-11-23 | 中国水产科学研究院淡水渔业研究中心 | Construction method for lentiviral vector containing IGF2b gene |
| CN102363792A (en) * | 2011-11-11 | 2012-02-29 | 中国水产科学研究院淡水渔业研究中心 | Lentivirus method for preparing IGF2b transgenic fish |
| CN102604897A (en) * | 2012-03-28 | 2012-07-25 | 中国水产科学研究院淡水渔业研究中心 | Application of lentivirus containing carp IGF2b gene in increase of carp meat yield |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250912A (en) * | 2011-06-10 | 2011-11-23 | 中国水产科学研究院淡水渔业研究中心 | Construction method for lentiviral vector containing IGF2b gene |
| CN102363792A (en) * | 2011-11-11 | 2012-02-29 | 中国水产科学研究院淡水渔业研究中心 | Lentivirus method for preparing IGF2b transgenic fish |
| CN102604897A (en) * | 2012-03-28 | 2012-07-25 | 中国水产科学研究院淡水渔业研究中心 | Application of lentivirus containing carp IGF2b gene in increase of carp meat yield |
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| 于飞等: "大菱鲆不同进口群体杂交后代的早期生长差异", 《水产学报》 * |
| 刘伟: "3个鲤群体的卫星标记与生长形状相关性分析", 《南方水产科学》 * |
| 吴延熊等: "《统计大师与统计分析》", 31 August 2006, 湖北科学技术出版社 * |
| 明俊超等: "6个不同鲤群体的形态差异分析", 《广东海洋大学学报》 * |
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