CN103551090A - Method for preparing mixed micelle of lecithin/nonionic surface active agent by virtue of direct dispersion method - Google Patents
Method for preparing mixed micelle of lecithin/nonionic surface active agent by virtue of direct dispersion method Download PDFInfo
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- CN103551090A CN103551090A CN201310559978.7A CN201310559978A CN103551090A CN 103551090 A CN103551090 A CN 103551090A CN 201310559978 A CN201310559978 A CN 201310559978A CN 103551090 A CN103551090 A CN 103551090A
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Abstract
The invention relates to a method for preparing mixed micelle of lecithin/nonionic surface active agent by virtue of a direct dispersion method and belongs to a surface active agent. According to the method, clear micelle is prepared from lecithin and a nonionic surface active agent by virtue of the direct dispersion method, and an organic solvent is not required in the process. The light transmittance of the prepared mixed micelle is 99.1+/-0.92%, and the average grain diameter is 10.25+/-0.86nm.
Description
Technical field
Dispersion method is prepared lecithin/non-ionic surface active agent mixed micelle method, belongs to technical field of surfactant.
Technical background
Micella is a kind of specific accumulation shape of surfactant in solvent.Surfactant molecule is amphiphile, amphiphilic molecule, when concentration reaches critical micelle concentration (critical micelle concentration, CMC) in selective solvent, can form micella.Micella belongs to a kind of association system.This system is different from general colloid and is that it is the thermodynamic stable system that water and surfactant form, i.e. this association is spontaneous and reversible.Micella is conventionally very little, is dispersed in uniformly in surfactant solution, generally at 0.1-100nm.About mixed micelle report more be mainly the film evaporation method preparation of lecithin/ionic surface active agent mixed micelle, cause the narrow and residue problem of film evaporation method solvent in mixed micelle of the optional material ranges of mixed micelle.The invention discloses a kind of direct dispersion method and prepare lecithin/non-ionic surface active agent mixed micelle method, overcome above shortcoming, to the embedding of food sensitive component and medicament solubilization technology, provide feasible new technology.
Summary of the invention
Technical scheme of the present invention: the object of the present invention is to provide a kind of direct dispersion method to prepare lecithin/non-ionic surface active agent mixed micelle method.Precision takes 1 gram of lecithin and 2 grams of non-ionic surface active agents are directly dispersed in the phosphate buffer of 100mL0.067mol/LpH7.4, first carry out manually pre-dispersed emulsion extremely uniformly, be then transferred in enzyme reactor, add stirring rotator, fill nitrogen, sealing.Be placed on magnetic stirring apparatus, under 50 ℃ of-70 ℃ of hydration temperatures, stir 3-4h, through high-speed shearing emulsion machine, take rotating speed as 22000r/min emulsification shearing, emulsification shear time is 6-20min.After shearing, adjust 37-45 ℃ of temperature and continue to stir 24h, obtain the micellar solution of clarification, cross the miillpore filter of 0.22 μ m, obtain lecithin mixed micelle solution.Prepared mixed micelle light transmittance is 99.1+0.92%, and average grain diameter is 10.25 ± 0.86nm.
(1) lecithin using is soybean lecithin, and in lecithin, the content of soy phosphatidylcholine is greater than 90%;
(2) non-ionic surface active agent using can be a kind of in following table surface-active agent: ten polyglycereol monolaurates (L-7D), ten polyglycereol list myristinates (M-10D), ten polyglycereol monostearates (SWA-10D), Surfhope SE Cosme C 1216 (SL), sucrose palmitate (P-1670), sucrose stearate (SS), Cremophor RH40 etc.
Beneficial effect of the present invention: a kind of direct dispersion method is prepared lecithin/non-ionic surface active agent mixed micelle method, for the preparation of lecithin/non-ionic surface active agent mixed micelle provides a kind of feasible method, the residue problem of the conventional films evaporation solvent that the method is avoided in mixed micelle, and a kind of new lecithin/non-ionic surface active agent mixed micelle is provided, for food processing and medicament solubilization tool, have significant practical applications.
The specific embodiment
Embodiment 1
Precision takes 1 gram of lecithin and 2 gram of ten polyglycereol monolaurate (L-7D) is directly dispersed in the phosphate buffer of 100mL0.067mol/LpH7.4, first carry out manually pre-dispersed emulsion extremely uniformly, be then transferred in enzyme reactor, add stirring rotator, fill nitrogen, sealing.Be placed on magnetic stirring apparatus, under 50 ℃ of hydration temperatures, stir 4h, through high-speed shearing emulsion machine, take rotating speed as 22000r/min emulsification shearing, emulsification shear time is 20min.After shearing, adjust 37 ℃ of temperature and continue to stir 24h, obtain the micellar solution of clarification, cross the miillpore filter of 0.22 μ m, obtain lecithin mixed micelle solution.Prepared mixed micelle light transmittance is 99.1+0.92%, and average grain diameter is 10.25 ± 0.86nm.
Embodiment 2
Precision takes 1 gram of lecithin and 2 gram of ten polyglycereol monostearate is directly dispersed in the phosphate buffer of 100mL0.067mol/L pH7.4, first carry out manually pre-dispersed emulsion extremely uniformly, be then transferred in enzyme reactor, add stirring rotator, fill nitrogen, sealing.Be placed on magnetic stirring apparatus, under 70 ℃ of hydration temperatures, stir 3h, through high-speed shearing emulsion machine, take rotating speed as 22000r/min emulsification shearing, emulsification shear time is 6min.After shearing, adjust temperature 45 C and continue to stir 24h, obtain the micellar solution of clarification, cross the miillpore filter of 0.22 μ m, obtain lecithin mixed micelle solution.Prepared mixed micelle light transmittance is 99.3 ± 0.77%, and average grain diameter is 10.12 ± 0.25nm.
Embodiment 3
Precision takes 1 gram of lecithin and 2 gram of ten polyglycereol monostearate is directly dispersed in the phosphate buffer of 100mL0.067mol/L pH7.4, first carry out manually pre-dispersed emulsion extremely uniformly, be then transferred in enzyme reactor, add stirring rotator, fill nitrogen, sealing.Be placed on magnetic stirring apparatus, under 60 ℃ of hydration temperatures, stir 4h, through high-speed shearing emulsion machine, take rotating speed as 22000r/min emulsification shearing, emulsification shear time is 15min.After shearing, adjust 37 ℃ of temperature and continue to stir 24h, obtain the micellar solution of clarification, cross the miillpore filter of 0.22 μ m, obtain lecithin mixed micelle solution.Prepared mixed micelle light transmittance is 95.7 ± 0.83%, and average grain diameter is 14.9 ± 0.23nm.Prepared mixed micelle light transmittance is 99.4 ± 0.86%, and average grain diameter is 10.10 ± 0.92nm.
Claims (8)
1. a direct dispersion method is prepared lecithin/non-ionic surface active agent mixed micelle method, it is characterized in that precision takes 1 gram of lecithin and 2 grams of non-ionic surface active agents are directly dispersed in the phosphate buffer of 100mL0.067mol/L pH7.4, first carry out manually pre-dispersed to uniform emulsion, then be transferred in enzyme reactor, add stirring rotator, fill nitrogen, sealing.Be placed on magnetic stirring apparatus, under 50 ℃ of-70 ℃ of hydration temperatures, stir 3-4h, through high-speed shearing emulsion machine, take rotating speed as 22000r/min emulsification shearing, emulsification shear time is 6-20min.After shearing, adjust 37-45 ℃ of temperature and continue to stir 24h, obtain the micellar solution of clarification, cross the miillpore filter of 0.22 μ m, obtain lecithin mixed micelle solution.Prepared mixed micelle light transmittance is 99.1 ± 0.92%, and average grain diameter is 10.25 ± 0.86nm.
(1) lecithin using is soybean lecithin, and in lecithin, the content of soy phosphatidylcholine is greater than 90%;
(2) non-ionic surface active agent using can be a kind of in following table surface-active agent: ten polyglycereol monolaurates (L-7D), ten polyglycereol list myristinates (M-10D), ten polyglycereol monostearates (SWA-10D), Surfhope SE Cosme C 1216 (SL), sucrose palmitate (P-1670), sucrose stearate (SS), Cremophor RH40 etc.
2. a kind of direct dispersion method according to claim 1 is prepared lecithin/non-ionic surface active agent mixed micelle method, it is characterized in that used lecithin is for soybean lecithin, and in lecithin, the content of soy phosphatidylcholine is greater than 90%.
3. a kind of direct dispersion method according to claim 1 is prepared lecithin/non-ionic surface active agent mixed micelle method, it is characterized in that used non-ionic surface active agent can be a kind of in following table surface-active agent: ten polyglycereol monolaurates (L-7D), ten polyglycereol list myristinates (M-10D), ten polyglycereol monostearates (SWA-10D), Surfhope SE Cosme C 1216 (SL), sucrose palmitate (P-1670), sucrose stearate (SS), Cremophor RH40 etc.
4. a kind of direct dispersion method according to claim 1 is prepared lecithin/non-ionic surface active agent mixed micelle method, it is characterized in that hydration temperature is 50 ℃-70 ℃.
5. a kind of direct dispersion method according to claim 1 is prepared lecithin/non-ionic surface active agent mixed micelle method, it is characterized in that hydration time is 3-4 hour.
6. a kind of direct dispersion method according to claim 1 is prepared lecithin/non-ionic surface active agent mixed micelle method, it is characterized in that emulsification shear time is 6-20min.
7. a kind of direct dispersion method according to claim 1 is prepared lecithin/non-ionic surface active agent mixed micelle method, and it is characterized in that shearing rear continuation, to stir the temperature of 24h be 37-45 ℃.
8. a kind of direct dispersion method according to claim 1 is prepared lecithin/non-ionic surface active agent mixed micelle method, it is characterized in that the preparation of micella has adopted direct dispersion method, in process, does not need with an organic solvent.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106539759A (en) * | 2015-09-17 | 2017-03-29 | 阿赖耶识（上海）生物技术有限公司 | A kind of non-vesicle type nano-particle of high stability and its application in treatment propionibacterium acness infection |
| CN106551902A (en) * | 2015-09-17 | 2017-04-05 | 阿赖耶识（上海）生物技术有限公司 | A kind of non-vesicle type nano-particle of high stable and its application in treatment microorganism infection |
-
2013
- 2013-11-08 CN CN201310559978.7A patent/CN103551090A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106539759A (en) * | 2015-09-17 | 2017-03-29 | 阿赖耶识（上海）生物技术有限公司 | A kind of non-vesicle type nano-particle of high stability and its application in treatment propionibacterium acness infection |
| CN106551902A (en) * | 2015-09-17 | 2017-04-05 | 阿赖耶识（上海）生物技术有限公司 | A kind of non-vesicle type nano-particle of high stable and its application in treatment microorganism infection |
| EP3351238A4 (en) * | 2015-09-17 | 2019-07-10 | Alaya (Shanghai) Bioscience Co., Ltd | Highly stable non-vesicular nanoparticles and application thereof in treating microbial infection |
| CN106551902B (en) * | 2015-09-17 | 2020-07-03 | 阿赖耶识（上海）生物技术有限公司 | High-stability non-vesicular nanoparticles and application thereof in treating microbial infection |
| CN106539759B (en) * | 2015-09-17 | 2020-07-03 | 阿赖耶识（上海）生物技术有限公司 | High-stability non-vesicular nanoparticles and application thereof in treating propionibacterium acnes infection |
| CN111759823A (en) * | 2015-09-17 | 2020-10-13 | 阿赖耶识（上海）生物技术有限公司 | High-stability non-vesicular nanoparticles and application thereof in treating microbial infection |
| US11266604B2 (en) * | 2015-09-17 | 2022-03-08 | Alaya (Shanghai) Bioscience Co., Ltd. | Highly stable non-vesicular nanoparticles and application thereof in treating microbial infection |
| CN111759823B (en) * | 2015-09-17 | 2022-12-30 | 阿赖耶识（上海）生物技术有限公司 | High-stability non-vesicular nanoparticles and application thereof in treating microbial infection |
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Application publication date: 20140205 |