CN103550392A - Method for preparing enteromorpha extract solution for inhibiting prorocentrum micans and phaeodactylum tricornutum bohlin - Google Patents
Method for preparing enteromorpha extract solution for inhibiting prorocentrum micans and phaeodactylum tricornutum bohlin Download PDFInfo
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- CN103550392A CN103550392A CN201310590730.7A CN201310590730A CN103550392A CN 103550392 A CN103550392 A CN 103550392A CN 201310590730 A CN201310590730 A CN 201310590730A CN 103550392 A CN103550392 A CN 103550392A
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Abstract
The invention discloses a method for preparing an enteromorpha extract solution for inhibiting prorocentrum micans and phaeodactylum tricornutum bohlin, belongs to the technical field of medicines, in particular relates to a method for preparing an atropa belladonna extract and provides a method for preparing the atropa belladonna extract, wherein the method is short in process flow, high in product purity, sewage-free, environment-friendly and pollution-free. The preparation method comprises the following steps: by taking herba belladonnae as a raw material, crushing the raw material, adding a sulfuric acid or hydrochloric acid solution for stirring, filtering the stirred solution, adsorbing and separating the filtered solution by using resin, eluting by using an ethanol solution, and concentrating the eluate to obtain the atropa belladonna extract. Because a normal-temperature acid solution extraction method is adopted, the dissolution of chlorophyll and impurities is small, and the atropa belladonna alkaloid is easily extracted from the acid solution, so that the active ingredient content in the extracted solution is high, and fewer impurities are contained in the extracted solution.
Description
Technical field
The invention belongs to medical technical field, be specifically related to a kind of preparation method of enteromorpha extract that suppresses ocean Prorocentrum and Phaeodactylum.
Background technology
Semen daturae is a kind of Solanaceae herbaceous plant, and effective ingredient is hyoscyamine, acts on identically with atropine, is mainly used in stomach spasm, Stomach duodenum ulcer, biliary colic, the stomachache that ureteral calculus etc. cause, the vomiting that gastritis and stomach spasm cause and diarrhoea.Domestic present extraction process mostly adopts 85% acetic acid percolation to extract belladonna extract, and still, the shortcoming of current technology is that extraction process is long, and chlorophyll is many, and except unclean, product impurity level is large.
Summary of the invention
The technical problem to be solved in the present invention is to provide the preparation method of the belladonna extract that a kind of technological process is short, purity is high, and the method is without blowdown, environment friendly and pollution-free.
The technical scheme of technical solution problem of the present invention is:
Belladonna extract preparation method of the present invention is: take Herba Belladonnae as raw material, by raw material pulverizing, it is 10--20 order fine powder, add sulphuric acid or hydrochloric acid solution to stir fine powder, filter and stir liquid, filtrate is used after resin absorption separation, use alcoholic solution eluting, then eluent is concentrated to containing alcohol amount 25--35%, obtain belladonna extract.
Sulfuric acid solution described in belladonna extract preparation method of the present invention refers to that concentration is the aqueous sulfuric acid of 0.05--0.5%.
Hydrochloric acid solution described in belladonna extract preparation method of the present invention refers to that adding concentration is the aqueous hydrochloric acid solution of 0.1--2%.
Add sulphuric acid or hydrochloric acid solution stirring described in belladonna extract preparation method of the present invention are: add at twice sulphuric acid or hydrochloric acid solution to stir, each time of stirring is 2--4 hour.
The above-mentioned method that adds at twice sulphuric acid or hydrochloric acid solution to stir is: add for the first time sulphuric acid or hydrochloric acid solution be raw material weight 10--12 doubly, filter; Add for the second time sulphuric acid or hydrochloric acid solution be raw material weight 6--8 doubly, filter; Merge and stir liquid; Each time of stirring is 2--4 hour.
Filtrate resin absorption described in belladonna extract preparation method of the present invention is to use D101 macroporous resin adsorption, absorption flow velocity 2--5BV/H, then be washed till PH=6--7 with the clear water of 6BV.
Described in belladonna extract preparation method of the present invention, with alcoholic solution eluting, be: with 75%--85% alcoholic solution eluting, elution flow rate is 3BV/H.
The invention has the beneficial effects as follows:
1, because the present invention adopts room temperature acid solution extraction method, chlorophyll and impurity stripping are few, and belladonna alkaloids is more easily extracted in acid solution, so effective component content is high in the solution extracting, impurity is few, and energy consumption is low;
2, in method of the present invention, after resin absorption, belladonna alkaloids is selectively adsorbed by resin, other impurity flows out with sour water and is not adsorbed, use again a small amount of alcoholic solution eluting, obtain in belladonna extract belladonna alkaloids content high, therefore, extract purity is relatively high, and cost is lower;
3, the inventive method acid solution used can reuse, next batch extract that acid solution used can use that last batch refers to adsorb through tree acid solution, so both saved water and acid, non-wastewater discharge again, can be to environment.
The specific embodiment
Below in conjunction with embodiment, the present invention is described in further details.
Embodiment 1:
1, get Semen daturae raw material 100kg, pulverize the order into 10--20;
2, the Semen daturae raw material of pulverizing is added 0.5% hydrochloric acid solution stir twice, each 3 hours, adding for the first time aqueous hydrochloric acid solution was 10 times of raw material weight, and adding for the second time aqueous hydrochloric acid solution is 6 times of raw material weight, merges and stirs liquid, filters;
3, filtrate is separated at hole resin absorption through D101, absorption flow velocity 3BV/H, then be washed till PH=6--7 with the clear water of 6BV;
4, with 4BV75% alcoholic solution eluting, elution flow rate is 3BV/H, collects eluent
5, eluent is concentrated into containing alcohol amount approximately 30%, obtains 13.6kg content and be 1.61% belladonna extract, the material content of take collects rate as 89.4%.
Embodiment 2:
1, get Semen daturae raw material 100kg, pulverize the order into 10--20;
2, the Semen daturae raw material of pulverizing is added 0.1% sulfuric acid solution stir twice, each 3 hours, adding for the first time aqueous sulfuric acid was 12 times of raw material weight, and adding for the second time aqueous sulfuric acid is 8 times of raw material weight, merges and stirs liquid, filters;
3, filtrate is separated at hole resin absorption through D101, absorption flow velocity 3BV/H, then be washed till PH=6--7 with the clear water of 6BV;
4, with 4BV80% alcoholic solution eluting, elution flow rate is 3BV/H, collects eluent;
5, eluent is concentrated into containing alcohol amount approximately 30%, obtains 14.9kg content and be 1.53% belladonna extract, the material content of take collects rate as 93.1%.
The said BVWei Bei of the present invention column volume unit.
Claims (7)
1. a preparation method of enteromorpha extract that suppresses ocean Prorocentrum and Phaeodactylum, it is characterized in that: take Herba Belladonnae as raw material, by raw material pulverizing, it is 10--20 order fine powder, add sulphuric acid or hydrochloric acid solution to stir fine powder, filter and stir liquid, filtrate, with after resin absorption separation, is used to alcoholic solution eluting, again eluent is concentrated to containing to containing alcohol amount 25--35%, obtains belladonna extract.
2. according to the said preparation method of claim 1, it is characterized in that: described sulfuric acid solution refers to that concentration is the aqueous sulfuric acid of 0.05--0.5%.
3. according to the said preparation method of claim 1, it is characterized in that: described hydrochloric acid solution refers to that concentration is the aqueous hydrochloric acid solution of 0.1--2%.
4. according to the said preparation method of claim 1, it is characterized in that: described add sulphuric acid or hydrochloric acid solution stirring are to add at twice sulphuric acid or hydrochloric acid solution to stir, and each time of stirring is 2--4 hour.
5. according to the said preparation method of claim 4, it is characterized in that: the described method that adds at twice sulphuric acid or hydrochloric acid solution to stir is: add for the first time sulphuric acid or hydrochloric acid solution be raw material weight 10--12 doubly, filter; Add for the second time sulphuric acid or hydrochloric acid solution be raw material weight 6--8 doubly, filter; Merge and stir liquid; Each time of stirring is 2--4 hour.
6. according to the said extract of claim 1, it is characterized in that: described filtrate resin absorption is to use D101 macroporous resin adsorption, absorption flow velocity 2--5BV/H, then be washed till PH=6--7 with the clear water of 6BV.
7. according to the said extract of claim 1, it is characterized in that: described with alcoholic solution eluting is: with 75%--85% alcoholic solution eluting, elution flow rate is 3BV/H.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310590730.7A CN103550392A (en) | 2013-11-22 | 2013-11-22 | Method for preparing enteromorpha extract solution for inhibiting prorocentrum micans and phaeodactylum tricornutum bohlin |
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| CN201310590730.7A CN103550392A (en) | 2013-11-22 | 2013-11-22 | Method for preparing enteromorpha extract solution for inhibiting prorocentrum micans and phaeodactylum tricornutum bohlin |
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| CN103550392A true CN103550392A (en) | 2014-02-05 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104173578A (en) * | 2014-09-10 | 2014-12-03 | 湖南科技学院 | Preparation method of belladonna extract |
| CN105879427A (en) * | 2016-05-09 | 2016-08-24 | 青岛海大生物集团有限公司 | Preparation method of enteromorpha biological anti-algae agent |
-
2013
- 2013-11-22 CN CN201310590730.7A patent/CN103550392A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104173578A (en) * | 2014-09-10 | 2014-12-03 | 湖南科技学院 | Preparation method of belladonna extract |
| CN104173578B (en) * | 2014-09-10 | 2018-09-14 | 湖南科技学院 | Extractum belladonnae preparation method |
| CN105879427A (en) * | 2016-05-09 | 2016-08-24 | 青岛海大生物集团有限公司 | Preparation method of enteromorpha biological anti-algae agent |
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Application publication date: 20140205 |
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