CN103536291B - Body surface sebum distribution testing method - Google Patents
Body surface sebum distribution testing method Download PDFInfo
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- CN103536291B CN103536291B CN201310243160.4A CN201310243160A CN103536291B CN 103536291 B CN103536291 B CN 103536291B CN 201310243160 A CN201310243160 A CN 201310243160A CN 103536291 B CN103536291 B CN 103536291B
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Abstract
The invention relates to a body surface sebum distribution testing method which utilizes body surface sebum distribution of fluorescence. The method comprises the steps of obtaining a body surface fluorescence image, wherein an imaging system forms the body surface fluorescence image of a testee; extracting bright and white fluorescence points, wherein an image analysis system is used for substracting the bright and white fluorescence points from the body surface fluorescence image; obtaining body surface sebum distribution, wherein the bright and white fluorescence point distribution is used as the body surface sebum distribution, moreover, the higher the area ratio or the strength of the bright and white fluorescence points is, the higher the sebum amount of the area, corresponding to the bright and white fluorescence points, of the body surface of the testee is.
Description
Technical field
The present invention relates to the assay method of body surface sebum distribution, the survey being more particularly to distributed using the body surface sebum of fluorescence
Determine method.
Background technology
The excessive secretion of the sebum at the sebum secretion position of body surface, such as face, chest, lower abdomen or back etc. is generally and body
Some diseases of epidermis skin are closely related, and for example, whelk is a kind of because sebum is secreted in a large number, and excessive sebum can not be timely
Discharge, deposit in hair follicle, so as to cause hair follicle inflammatory reaction and caused acne symptom.Accordingly, it would be desirable to determine body surface sebum
It is distributed to be treated for the region more than body surface sebum amount.
In order to determine the distribution of body surface sebum, typically using existing sebum tester(sebumeter).Specifically, skin
Fat tester is, based on the instrument of photometer principle, the special delustring adhesive tape of thickness 0.1mm to be affixed on into a part of region of body surface
On, after body surface sebum is absorbed, the adhesive tape becomes translucent adhesive tape, and its light transmission capacity can change, the sebum of absorption
Amount is bigger, and light transmission capacity is bigger, and thus sebum tester can measure the sebum amount on the region according to the change of light transmission capacity.
However, in the method using sebum tester, one-shot measurement can only be carried out to a pocket of body surface, if necessary to determine
The sebum distribution of whole body surface, then need multiple measurement, so as to cause measuring process numerous and diverse and elapsed time length problem.
The content of the invention
Therefore, the present invention is the invention completed in view of the problems referred to above, can reliably and rapidly be surveyed with providing one kind
For the purpose of determining the assay method of body surface sebum distribution.
The present inventors is using the Wood being widely used in skin diagnosis(Wood)Lamp, to body surface irradiation from wood's lamp
The long wavelength ultraviolet light for sending(UVA light)In the case of, it was found that the fluorescence of shades of colour can be sent from body surface.
Wood's lamp using high-pressure mercury-vapor lamp as transmitting light source, by the wave filter of the barium silicate containing 9% nickel oxide(5
Moral wave filter), so as to send the light of the wavelength of 320nm~400nm, peak value is 365nm.
Further, the present inventors is after it have studied lot of documents, it was found that body surface sebum can cause to produce title in body surface
For the hair follicle fluorescent substance of porphyrin, and the hair follicle fluorescent substance can launch glimmering under the exciting of the exciting light of UVA light etc.
Light.Therefore, the present inventors thinks the fluorescence of above-mentioned shades of colour due to the hair follicle fluorescent substance caused by sebum, and
Correlation is there may be between body surface sebum and fluorescence.
The present inventors on the basis of above-mentioned opinion, by sebum tester(sebumeter)N name experimenters are determined respectively
Body surface predetermined region sebum amount and obtain the 1st data group, by image analysis system(For example, it is made up of software I mageJ)
Determine the area ratio or intensity of the phosphor dot of the different colours of the predetermined region of the body surface of N name experimenters and obtain the 2nd number
According to group, using SPSS(Statistical Product and Service Solutions, statistical product and service solution party
Case)Software is calculated the relation between the 1st data group and the 2nd data group, it was found that the body measured by sebum tester
There is positive line between the area ratio or intensity of the phosphor dot of epidermal lipid amount and the brilliant white obtained by image analysis software
Property correlation(That is, body surface sebum distribution is consistent, and the area of the phosphor dot of brilliant white with the distribution of the phosphor dot of brilliant white
Ratio or intensity are higher, higher corresponding to the sebum amount in the region of the body surface of the experimenter of the phosphor dot of brilliant white), and by skin
The body surface sebum amount that fat tester is measured and other colors obtained by image analysis software(For example, it is orange red)Phosphor dot
There is no linear dependence between area ratio or intensity, therefore, the present invention chooses the phosphor dot that particular color is brilliant white,
Correlation between body surface sebum and brilliant white fluorescence is carried out to inquire into research, so as to complete the present invention.
That is, the assay method of body surface sebum distribution involved in the present invention, is the survey being distributed using the body surface sebum of fluorescence
Determine method, including:Body surface fluoroscopic image acquisition step, by imaging system the body surface fluoroscopic image of experimenter is formed;Brilliant white is glimmering
Luminous point extraction step, by image analysis system, extracts the phosphor dot of brilliant white from the body surface fluoroscopic image;And body surface skin
Fat is distributed acquisition step, is distributed the distribution of the phosphor dot of the brilliant white as the body surface sebum, and the brilliant white
The area ratio or intensity of phosphor dot is higher, corresponding to the region of the body surface of the experimenter of the phosphor dot of the brilliant white
Sebum amount it is higher.
As described above, the assay method being distributed according to body surface sebum involved in the present invention, by colorful digital video camera etc.
Camera unit the body surface of the experimenter under Wood light irradiation is photographed, obtain experimenter body surface fluoroscopic image, so as to
The distribution of the brilliant white phosphor dot in the body surface fluoroscopic image can be utilized, the distribution of body surface sebum is obtained, therefore, compared to existing
Technology, without the need for the skin of directly contact experimenter, can reliably and rapidly determine the distribution of body surface sebum, be additionally, since utilization
Image analysis system extracts the phosphor dot of brilliant white from body surface fluoroscopic image, therefore, it can determine body surface sebum point in real time
Cloth.
In addition, in the assay method of above-mentioned body surface sebum distribution involved in the present invention, it is preferable that the brilliant white is glimmering
Luminous point extraction step includes:Boundary value determines step, manually extracts the brilliant white of the specified quantity in the body surface fluoroscopic image
Phosphor dot and determined by described image analysis system the brilliant white phosphor dot rgb value, by the multiple rgb values for measuring
Maximum as upper bound limit value, using the minimum of a value in the multiple rgb values for measuring as lower bound limit value;And phosphor dot extracts step
Suddenly, the upper bound limit value and the lower bound limit value are set in described image analysis system as parameter, so as to by institute
State image analysis system and automatically extract the fluorescence that rgb value is the brilliant white below the upper bound limit value and more than the lower bound limit value
Point.So, by manually obtaining the phosphor dot of multiple brilliant whites first and calculating its rgb value, and by maximum therein with
And minimum of a value is set in image analysis system as parameter, such that it is able to extract brilliant white exactly by image analysis system
Phosphor dot, thus, it is possible to more reliably determine the distribution of body surface sebum, in addition, by manually setting appropriate boundary value, must
When wanting, phosphor dot can be divided into different colours to be analyzed.
In addition, in the assay method of above-mentioned body surface sebum distribution involved in the present invention, stablizing from measurement result
Property and analysis process high efficiency from the viewpoint of, it is preferable that in the boundary value setting procedure, manually extract 10~20
The phosphor dot of brilliant white.
In addition, in the assay method of above-mentioned body surface sebum distribution involved in the present invention, the fluorescence of the brilliant white
Point is that under the color space based on " sRGB IEC61966-2.1 ", it is 40~255 that R is 0~30, G, and B is 73~255
Phosphor dot.
In addition, in the assay method of above-mentioned body surface sebum distribution involved in the present invention, it is preferable that the imaging system
Including:Film studio, is surrounded with black curtain around the film studio;Light source, in being arranged at the film studio, receives to described
The body surface irradiation exciting light of examination person;Camera unit, in being arranged at the film studio, to after by the excitation from described
The fluorescence of the fluorescent substance transmitting on the body surface of experimenter is photographed, so as to obtain the body surface fluorogram of the experimenter
Picture.So, by being surrounded with black curtain around film studio, such that it is able in the dark room conditions surrounded by black curtain
In, to the exciting light that the body surface irradiation of experimenter is evenly distributed.
In addition, in the assay method of above-mentioned body surface sebum distribution involved in the present invention, it is preferable that the light source is associated with
Moral lamp, the exciting light is the ultraviolet light that wavelength is 320~400nm.Because wood's lamp has the advantages that noncontact, fanout free region etc.
And it is preferred that, in addition, by the ultraviolet light for making exciting light be that wavelength is 320~400nm, such that it is able to the wound for avoiding causing skin
Evil.
In addition, in the assay method of above-mentioned body surface sebum distribution involved in the present invention, it is preferable that glimmering in the body surface
In light image acquisition step, by changing distance or institute on the depth direction between the camera unit and the experimenter
The focal length of camera unit is stated, so as to adjust the definition of the body surface fluoroscopic image.
In addition, in the assay method of above-mentioned body surface sebum distribution involved in the present invention, it is preferable that described image is analyzed
System is made up of the image processing software of the area ratio with computational rules pixel and the function of intensity.In addition, the figure
As analysis system is preferably made up of image processing software ImageJ.Image processing software ImageJ is a common software, by U.S.
NIH of state(National Institutes of Health)Exploitation.Image processing software ImageJ has
For the image being taken into, specific necessary region, to carry out the function of pixel analysis, therefore, by the image processing software
It is easy that ImageJ is applied in body surface sebum is determined.
In addition, in the assay method of above-mentioned body surface sebum distribution involved in the present invention, also including:Body surface cleaning step
Suddenly, before the body surface fluoroscopic image acquisition step, the body surface of the experimenter is cleaned.Specifically, first, use
Water infiltration skin surface, and skin surface is cleaned with commercially available soap or cleaning agent, thereafter, rinsed out.
Certain hour, after such as 30 minutes, obtain body surface fluoroscopic image.Further, even if the present invention does not possess body surface cleaning step, also may be used
To carry out the measure of body surface sebum distribution.
Obtain body surface fluoroscopic image time be cleaning body surface after 30 minutes and before no clean body surface, as such, it is possible to arrange
Except the dust of fluorescent substance contained in such as air, the towel hair containing fluorescent substance etc. other factors it is dry
Disturb, such that it is able to more reliably determine the distribution of body surface sebum.
In addition, in the assay method of above-mentioned body surface sebum distribution involved in the present invention, it is preferable that the body surface is face
Portion.
According to the assay method that body surface sebum involved in the present invention is distributed, there is provided can reliably and rapidly determine body
The assay method of epidermal lipid distribution.The method of the present invention, can be extremely short compared with the sebum determination techniques being currently known
It is measured in time.In addition, the method for the present invention is not the method for making tools for measurement contact with skin or boning, but energy
It is enough to be non-contactly measured, therefore, the burden to the skin of person to be measured(stress)It is minimum.Further, since being noncontact
Measure, therefore, method according to the invention it is possible to carry out the measure gradually of the state of sebum.Even if further, to skin
It is coated with the state of emulsion or has carried out also carrying out the measure of sebum in the state of the cosmetic of various cosmetics etc..
Description of the drawings
Fig. 1 is the body surface for exciting the experimenter under light irradiation for representing respectively under common light irradiation and sending from wood's lamp
Photo figure.
Fig. 2 is the photo of the general configuration of the imaging system for representing involved in the present invention.
Fig. 3 is the schematic side view of the general configuration of the imaging system for representing involved in the present invention.
Fig. 4 be the sebum amount measured by sebumeter in the predetermined region at the wing of nose of the body surface for representing experimenter with
The chart of the relation between the area ratio of the phosphor dot of the brilliant white obtained by image analysis software.
Fig. 5 be the sebum amount measured by sebumeter in the predetermined region at the wing of nose of the body surface for representing experimenter with
The chart of the relation between the intensity of the phosphor dot of the brilliant white obtained by image analysis software.
Fig. 6 be the sebum amount measured by sebumeter in the predetermined region at the forehead of the body surface for representing experimenter with
The chart of the relation between the area ratio of the phosphor dot of the brilliant white obtained by image analysis software.
Fig. 7 be the sebum amount measured by sebumeter in the predetermined region at the forehead of the body surface for representing experimenter with
The chart of the relation between the intensity of the phosphor dot of the brilliant white obtained by image analysis software.
Fig. 8 be the sebum amount measured by sebumeter in the predetermined region at the wing of nose of the body surface for representing experimenter with
The chart of the relation between the area ratio of the orange-red phosphor dot obtained by image analysis software.
Fig. 9 be the sebum amount measured by sebumeter in the predetermined region at the wing of nose of the body surface for representing experimenter with
The chart of the relation between the intensity of the orange-red phosphor dot obtained by image analysis software.
Figure 10 be the sebum amount measured by sebumeter in the predetermined region at the forehead of the body surface for representing experimenter with
The chart of the relation between the area ratio of the orange-red phosphor dot obtained by image analysis software.
Figure 11 be the sebum amount measured by sebumeter in the predetermined region at the forehead of the body surface for representing experimenter with
The chart of the relation between the intensity of the orange-red phosphor dot obtained by image analysis software.
Figure 12 is the stream of the composition of the 1st embodiment of the assay method of the body surface sebum distribution for representing involved in the present invention
Cheng Tu.
Figure 13 is the stream of the composition of the 2nd embodiment of the assay method of the body surface sebum distribution for representing involved in the present invention
Cheng Tu.
Figure 14 is the stream of the composition of the 3rd embodiment of the assay method of the body surface sebum distribution for representing involved in the present invention
Cheng Tu.
Specific embodiment
Hereinafter, referring to the drawings, being preferred embodiment described in detail to the present invention.Here, saying in accompanying drawing
In bright, to key element mark identical symbol identically or comparably, the repetitive description thereof will be omitted.
(1st embodiment)
First, to Wood(Wood)The surface images of the experimenter under lamp are studied.The photo in the left side of Fig. 1 represents general
Thang-kng(Here is, for example, daylight)The body surface photo of the experimenter under irradiation, the photo on the right side of Fig. 1 is represented to be launched by wood's lamp
The exciting light for going out(Here is long wavelength ultraviolet light(UVA light))The body surface photo of the experimenter under irradiation.As shown in figure 1, this
A persons of good sense are found that the body surface of experimenter under the irradiation of the exciting light launched by wood's lamp, and almost everyone body surface is deposited
In the phosphor dot of brilliant white, orange red, blue-green, yellow etc..The present inventors for the phenomenon, have studied lot of documents it
Afterwards, it was found that body surface sebum can cause to produce the hair follicle fluorescent substance of referred to as porphyrin, and the hair follicle fluorescent substance in body surface
Fluorescence can be launched under the exciting of the exciting light of UVA light etc..Therefore, the present inventors thinks that the fluorescence of above-mentioned shades of colour rises
Cause there may be correlation in the hair follicle fluorescent substance caused by sebum between body surface sebum and fluorescence.
Then, the present inventors chooses the experimenter of 40 all skin races, using imaging system 1, obtains this 40
The fluoroscopic image of the body surface under the Wood light irradiation of experimenter.Here, the experimenter by choosing 40 all skin races, from
And make sample size and species sufficiently large, thus reliability is higher.
Fig. 2 is the photo of the general configuration of the imaging system 1 for representing involved in the present invention.Fig. 3 is represented involved by the present invention
And imaging system 1 general configuration schematic side view.As shown in FIG. 2 and 3, the imaging system 1 include film studio 20,
Head positioning device 30, camera unit 40 and light source 50.Black curtain is surrounded with around film studio 20.As photography
Unit 40, using colorful digital camera.As light source 50, using wood's lamp(Sanyo's system, compact blue-black lamp FPL27BLB,
The UVA light of 320~400nm of launch wavelength(Ultraviolet light), peak value 365nm).Wood's lamp using high-pressure mercury-vapor lamp as transmitting light source,
By the wave filter of the barium silicate containing 9% nickel oxide(Wood wave filter), so as to send the wavelength of 320nm~400nm
Light, peak value is 365nm.Due to wood's lamp there is noncontact, fanout free region etc. and it is preferred that, in addition, by making the exciting light be
Wavelength is the ultraviolet light of 320~400nm, such that it is able to the injury for avoiding causing skin.
Then, when the body surface 10 to experimenter is photographed, from the ultraviolet light that light source 50 is launched(Exciting light)Irradiation
To the body surface 10 of the experimenter being positioned on head positioning device 30, by the ultraviolet excitation after the body surface 10 from experimenter on
The fluorescence of fluorescent substance transmitting reflect to camera unit 40, it is tested so as to obtain while photographed by camera unit 40
The body surface fluoroscopic image of the body surface 10 of person.So, by being surrounded with black curtain around film studio, such that it is able to by black
In the dark room conditions that color curtain is surrounded, to the exciting light that the body surface irradiation of experimenter is evenly distributed.
Then, using sebum tester(sebumeter), measure the predetermined region of the body surface of experimenter(That is, spy is posted
The region of different delustring adhesive tape)On sebum amount.Also, by the use of image analysis software ImageJ as image analysis system, point
Do not choose the brilliant white and orange-red phosphor dot in the predetermined region, and calculate brilliant white and orange-red glimmering respectively
The area ratio and intensity of luminous point.
Here, as image analysis system, exemplified with image processing software ImageJ, but, as long as image analysis system
It is that the image processing software of function by the area ratio with computational rules pixel and intensity is constituted, is not limited to image
Process software I mageJ.Further, image processing software ImageJ is a common software, by NIH
(National Institutes of Health)Exploitation.Image processing software ImageJ has for the image being taken into,
Specific necessary region, to carry out the function of pixel analysis, and be one can with the common software of the rgb value of analysis of the image etc.,
Therefore, it is easy image processing software ImageJ to be applied in body surface sebum is determined.
Further, so-called body surface, is the sebum secretion position at face, chest, lower abdomen or the back of such as experimenter etc..
Fig. 4 and Fig. 5 are respectively being measured by sebumeter in the predetermined region at the wing of nose of the body surface for representing experimenter
Sebum amount and the brilliant white obtained by image analysis software phosphor dot area ratio/intensity between relation chart.
Fig. 6 and Fig. 7 are respectively the sebum amounts measured by sebumeter in the predetermined region at the forehead of the body surface for representing experimenter
And the chart of the relation between the area ratio/intensity of the phosphor dot of the brilliant white obtained by image analysis software.Fig. 8 and figure
9 be respectively the sebum amount measured by sebumeter in the predetermined region at the wing of nose of the body surface for representing experimenter with by image
The chart of the relation between the area ratio/intensity of the orange-red phosphor dot that analysis software is obtained.Figure 10 and Figure 11 distinguishes
It is that the sebum amount measured by sebumeter in the predetermined region at the forehead of the body surface for representing experimenter is soft with by graphical analysis
The chart of the relation between the area ratio/intensity of the orange-red phosphor dot that part is obtained.From Fig. 4~Fig. 7, either exist
At the wing of nose or at forehead, there is positive linear phase between sebum amount and the area ratio/intensity of the phosphor dot of brilliant white
Guan Xing(Coefficient correlation is respectively 0.682,0.612,0.656,0.586).Contrary, from Fig. 8~Figure 11, either in nose
At the wing or at forehead, there is no correlation between sebum amount and the area ratio/intensity of orange-red phosphor dot.
As previously discussed, the present inventors is for 40 experimenters, by sebum tester(sebumeter)40 are determined respectively
Name experimenter body surface predetermined region sebum amount and obtain the 1st data group, by image analysis system(For example, by software
ImageJ is constituted)Determine the area ratio or intensity of the phosphor dot of the different colours of the predetermined region of the body surface of 40 experimenters
And the 2nd data group is obtained, using SPSS(Statistical Product and Service Solutions, statistical product with
Service solution)After software is calculated the relation between the 1st data group and the 2nd data group, it was found that by sebum
Body surface sebum amount and the area ratio or intensity of the phosphor dot of the brilliant white obtained by image analysis software that tester is measured
Between there is positive linear dependence, i.e. it is consistent, and brilliant white with the distribution of the phosphor dot of brilliant white that body surface sebum is distributed
Phosphor dot area ratio or intensity it is higher, corresponding to the sebum in the region of the body surface of the experimenter of the phosphor dot of brilliant white
Amount is higher, and by sebum tester the body surface sebum amount for measuring and other colors obtained by image analysis software(For example, it is orange red
Color)Phosphor dot area ratio or intensity between there is no linear dependence, therefore, the present inventors think determine brilliant white
The distribution of the phosphor dot of color is an effective method for determining the distribution of body surface sebum.
In the present embodiment, the assay method of body surface sebum distribution involved in the present invention is using the body surface skin of fluorescence
The assay method of fat distribution.
Figure 12 is the flow chart of the composition of the assay method of the body surface sebum distribution for representing involved in the present invention.Such as Figure 12 institutes
Show, the assay method of body surface sebum distribution involved in the present invention includes:Body surface fluoroscopic image acquisition step, by imaging system shape
Into the body surface fluoroscopic image of experimenter;Brilliant white phosphor dot extraction step, by image analysis system, carries from body surface fluoroscopic image
Take the phosphor dot of brilliant white;And body surface sebum distribution acquisition step, using the distribution of the phosphor dot of brilliant white as body surface sebum
Distribution, and the area ratio or intensity of the phosphor dot of brilliant white are higher, corresponding to the body of the experimenter of the phosphor dot of brilliant white
The sebum amount in the region of table is higher.
In addition, in the present embodiment, the phosphor dot of brilliant white is empty in the color based on " sRGBIEC61966-2.1 "
Between under, it is 40~255 that R is 0~30, G, and B is 73~255 phosphor dot.On the other hand, orange-red phosphor dot is in base
Under the color space of " sRGB IEC61966-2.1 ", it is 28~255 that R is 35~255, G, and B is 51~157 fluorescence
Point.Here, " sRGB IEC61966-2.1 " is the standard color space of the display for using under a windows environment.Here,
RGB is referred to as coloured light three primary colors, respectively red, green, blue, and its numerical value is R:0-255, G:0-255, B:0-255.
Further, in the present embodiment, using the color space based on " sRGB IEC61966-2.1 ", but, do not limit
Due to this, it would however also be possible to employ the color based on " Adobe RGB (1998) ", " AppleRGB " or " ColorMatch RGB " etc.
Space.Using the color space based on " Adobe RGB (1998) ", " AppleRGB " or " ColorMatch RGB " etc.
In the case of, the phosphor dot of the color of brilliant white, orange red etc., its R, G, B value is based on " sRGB IEC61966-2.1 " with adopting
The situation of color space is different.
According to the assay method of such body surface sebum distribution, by the camera unit of colorful digital video camera etc. to wood's lamp
The body surface of the experimenter under irradiation is photographed, and the body surface fluoroscopic image of experimenter is obtained, such that it is able to using the body surface fluorescence
The distribution of the brilliant white phosphor dot in image, obtains the distribution of body surface sebum, therefore, compared to prior art, without the need for directly contact
The skin of experimenter, can reliably and rapidly determine the distribution of body surface sebum, be additionally, since using image analysis system from body surface
The phosphor dot of brilliant white is extracted in fluoroscopic image, therefore, it can determine the distribution of body surface sebum in real time.
In addition, in the assay method of above-mentioned body surface sebum distribution involved in the present invention, taking in body surface fluoroscopic image
In step, it is also possible to by change the distance on the depth direction Y between camera unit 40 and the body surface 10 of experimenter or
The focal length of camera unit 40, so as to adjust the definition of body surface fluoroscopic image.
(2nd embodiment)
Figure 13 is the stream of the composition of the 2nd embodiment of the assay method of the body surface sebum distribution for representing involved in the present invention
Cheng Tu.The composition and the body surface involved by the 1st embodiment of the assay method of the body surface sebum distribution involved by the 2nd embodiment
The composition of the assay method of sebum distribution is roughly the same, thus the description thereof will be omitted.Hereinafter, said for both differences
It is bright.
Body surface skin involved by the assay method and the 1st embodiment of the body surface sebum distribution involved by the 2nd embodiment
The difference of the assay method of fat distribution is that brilliant white phosphor dot extraction step also includes:Boundary value determines step, artificial to extract
The phosphor dot of the brilliant white of the specified quantity in body surface fluoroscopic image simultaneously determines the phosphor dot of the brilliant white by image analysis system
Rgb value, using the maximum in the multiple rgb values for measuring as upper bound limit value, the minimum of a value in the multiple rgb values for measuring is made
For lower bound limit value;And phosphor dot extraction step, upper bound limit value and lower bound limit value are set in into graphical analysis as parameter
In system, so as to automatically extract rgb value for the brilliant white below the limit value of the upper bound and more than lower bound limit value by image analysis system
Phosphor dot.
Here, so-called " the artificial phosphor dot for extracting brilliant white ", refers to by discernable by eye and obtains the phosphor dot of brilliant white.
So, by manually extracting the phosphor dot of multiple brilliant whites first and calculating its rgb value, and by maximum therein
Value and minimum of a value are set in image analysis system as parameter, such that it is able to extract bright exactly by image analysis system
The phosphor dot of white, thus, it is possible to the distribution of body surface sebum is more reliably determined, in addition, by manually setting appropriate boundary
Value, phosphor dot if necessary, can be divided into different colours to be analyzed.
In addition, in the assay method of the body surface sebum distribution involved by the 2nd embodiment, it is also possible in boundary value setting
In step, from from the viewpoint of the high efficiency of the stability of measurement result and analysis process, 10~20 brilliant whites are manually extracted
The phosphor dot of color.But, the number of the phosphor dot of brilliant white is not restricted, as long as the phosphor dot of brilliant white can be obtained
The scope of rgb value, its number is not limited to 10~20.
(3rd embodiment)
Figure 14 is the stream of the composition of the 3rd embodiment of the assay method of the body surface sebum distribution for representing involved in the present invention
Cheng Tu.The composition and the body surface involved by the 1st embodiment of the assay method of the body surface sebum distribution involved by the 3rd embodiment
The composition of the assay method of sebum distribution is roughly the same, thus the description thereof will be omitted.Hereinafter, said for both differences
It is bright.
Involved by the composition and the 1st embodiment of the assay method of the body surface sebum distribution involved by the 3rd embodiment
The difference of the composition of the assay method of body surface sebum distribution is also to include:Body surface cleaning step, obtains in body surface fluoroscopic image
Before step, the body surface of multiple experimenters is cleaned.Specifically, first, water infiltration skin surface is used, and with commercially available
Soap or cleaning agent are cleaned to skin surface, thereafter, are rinsed out.In certain hour, after such as 30 minutes, obtain
Body surface fluoroscopic image.Obtain body surface fluoroscopic image time be body surface cleaning after 30 minutes rather than body surface cleaning before, so, can
The other factors of contained dust, the towel hair containing fluorescent substance of fluorescent substance etc. in exclude such as air
Interference, such that it is able to more reliably determine the distribution of body surface sebum.
More than, each embodiment of the assay method of body surface sebum distribution involved in the present invention is illustrated,
But, the present invention is not limited to above-mentioned embodiment, and those skilled in the art are in the connotation and model without departing from the present invention
The present invention can be deformed and changed as needed in the case of enclosing.These deformations and change each fall within the scope of the present invention
It is interior.
Claims (12)
1. the assay method that a kind of body surface sebum is distributed, it is characterised in that
It is the assay method being distributed using the body surface sebum of fluorescence,
Including:
Body surface fluoroscopic image acquisition step, by imaging system the body surface fluoroscopic image of experimenter is formed;
Brilliant white phosphor dot extraction step, by image analysis system, extracts the fluorescence of brilliant white from the body surface fluoroscopic image
Point;And
Body surface sebum is distributed acquisition step, is distributed the distribution of the phosphor dot of the brilliant white as the body surface sebum, and institute
The area ratio or intensity for stating the phosphor dot of brilliant white is higher, corresponding to the experimenter of the phosphor dot of the brilliant white
The sebum amount in the region of body surface is higher.
2. the assay method that body surface sebum as claimed in claim 1 is distributed, it is characterised in that
The brilliant white phosphor dot extraction step includes:
Boundary value determines step, manually extracts the phosphor dot of the brilliant white of specified quantity in the body surface fluoroscopic image and by institute
The rgb value that image analysis system determines the phosphor dot of the brilliant white is stated, the maximum in the multiple rgb values that will be measured is used as the upper bound
Limit value, using the minimum of a value in the multiple rgb values for measuring as lower bound limit value;And
Phosphor dot extraction step, the upper bound limit value and the lower bound limit value are set in described image analysis as parameter
In system, so as to by described image analysis system automatically extract rgb value for below the upper bound limit value and the lower bound limit value with
On brilliant white phosphor dot.
3. the assay method that body surface sebum as claimed in claim 2 is distributed, it is characterised in that
In the boundary value determines step, the phosphor dot of 10~20 brilliant whites is manually extracted.
4. the assay method that the body surface sebum as described in any one in claims 1 to 3 is distributed, it is characterised in that
The phosphor dot of the brilliant white is that R is 40 for 0~30, G under the color space based on " sRGB IEC61966-2.1 "
~255, and B is 73~255 phosphor dot.
5. the assay method that body surface sebum as claimed in claim 1 is distributed, it is characterised in that
The imaging system includes:
Film studio, is surrounded with black curtain around the film studio;
Light source, the body surface irradiation exciting light in being arranged at the film studio, to the experimenter;And
Camera unit, in being arranged at the film studio, to after by the excitation from the body surface of the experimenter
The fluorescence of fluorescent substance transmitting is photographed, so as to obtain the body surface fluoroscopic image of the experimenter.
6. the assay method that body surface sebum as claimed in claim 5 is distributed, it is characterised in that
The light source is wood's lamp,
The exciting light is the ultraviolet light that wavelength is 320~400nm.
7. the assay method that body surface sebum as claimed in claim 1 is distributed, it is characterised in that
Described image analysis system is by the area ratio with computational rules pixel and the image processing software of the function of intensity
Constitute.
8. the assay method that body surface sebum as claimed in claim 5 is distributed, it is characterised in that
In the body surface fluoroscopic image acquisition step, by changing the depth side between the camera unit and the experimenter
Distance upwards or the focal length of the camera unit, so as to adjust the definition of the body surface fluoroscopic image.
9. the assay method that body surface sebum as claimed in claim 1 is distributed, it is characterised in that
Also include:Body surface cleaning step, before the body surface fluoroscopic image acquisition step, is carried out to the body surface of the experimenter
Cleaning.
10. the assay method that body surface sebum as claimed in claim 9 is distributed, it is characterised in that
The body surface cleaning step is the step of being cleaned with soap or remover.
The assay method of the 11. body surface sebum distributions as described in any one in claims 1 to 3,5~10, its feature exists
In,
The body surface is face.
The assay method of 12. body surface sebum distributions as claimed in claim 4, it is characterised in that
The body surface is face.
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