CN103529170B - A kind of on-line checking automatic control system of starch saccharification finishing point - Google Patents
A kind of on-line checking automatic control system of starch saccharification finishing point Download PDFInfo
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- CN103529170B CN103529170B CN201310496637.XA CN201310496637A CN103529170B CN 103529170 B CN103529170 B CN 103529170B CN 201310496637 A CN201310496637 A CN 201310496637A CN 103529170 B CN103529170 B CN 103529170B
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Abstract
The invention discloses online test method and the automatic control system of a kind of starch saccharification finishing point, the method is: measure glucose and the content of reducing sugar of saccharified liquid；By formula E=CG/CRCalculating, when E reaches 0.98 0.99 intervals, then can determine that arrival saccharification terminal, E states saccharification terminal, CGRepresent glucose content, CRRepresent content of reducing sugar；This system includes: main control processor, full-automatic Iron mud, bio-sensing analyzer, saccharification conditional execution unit.The present invention, by measuring glucose, the content of reduced sugar in saccharified liquid, calculates glucose and reduced sugar ratio, determines saccharification terminal；Immobilised enzymes glucose biological sensing analyzer and Iron mud can carry out the mensuration of glucose and reduced sugar, method is simple, quickly, accurately, and be not required to measure dry matter content, possess the feasibility in actual production and practicality, preferably resolve existing assay method operation complexity, inefficiency, the problem limiting the development of sugar industry.
Description
Technical field
The invention belongs to starch saccharification finishing point detection technique field, particularly relate to online test method and the automatic control system of a kind of starch saccharification finishing point.
Background technology
At present, starch sugaring uses enzyme method technique more, through liquefaction and saccharifying, Starch Hydrolysis is become glucose, and in liquefaction operation, starch becomes dextrin and the less molecular product of compound sugar scope through a-amylorrhexis；Saccharification operation is then to utilize glucoamylase further these products to be hydrolyzed into glucose.
Mashing operation process is to introduce in saccharifying tank by liquefied starch, is adjusted to suitable temperature and pH value, adds the saccharidase preparation of requirement, keeps certain time, makes to reach the highest glucose content, obtain saccharified liquid；
After the glucose content that saccharified liquid reaches the highest, reaction should be stopped, otherwise, glucose content tends to reduce, this is because glucose generation recombination reaction, a part of glucose combines the compound carbohydrates such as generation isomaltose again, and this reaction is the most notable in the case of higher enzyme concentration and concentration of substrate, and glucoamylase has catalytic action for the recombination reaction of glucose；
Saccharification reaction is terminated in time to improving saccharification efficiency, shortening the production cycle, ensure that saccharification liquid quality has important effect at saccharification reaction end, in actual production, generally use DE value (also referred to as dextrose equivalent) or DX value (glucose) represents amylolytic degree or saccharification degree, control saccharification terminal
DE value: in saccharified liquid, reducing sugar (with glucose meter) accounts for the percentage of dry,
DE value=reduced sugar (C ")/dry (W ') × liquid glucose proportion (d) } × 100%
Wherein: reduced sugar Pei's Lin Shi method or iodometric determination, concentration represents: glucose g/100ml liquid glucose；Dry abbe's refractometer measures, and concentration represents: dry g/100g liquid glucose；
DX value: in liquid glucose, glucose content accounts for the percentage of dry,
DX value=glucose (C ")/dry (W ') × liquid glucose proportion (d) } × 100%；
In saccharifying, the actual content of glucose is less than dextrose equivalent (reduced sugar), because also reproducibility compound sugar exists, along with increasing of saccharification degree, the difference of the two gradually decreases, therefore, use glucose and reduced sugar ratio, i.e. DX/DE ratio, it may be determined that Mashing process process and saccharification terminal, in current domestic stable production technology, the glucose content DX of saccharified liquid is more than 95.5%, and dextrose equivalent DE is generally higher than 98%, and DX/DE ratio is more than 97.5%.
Existing assay method operation complexity, inefficiency, limit the development of sugar industry.
Summary of the invention
The purpose of the embodiment of the present invention is to provide online test method and the automatic control system of a kind of starch saccharification finishing point, it is intended to solve existing assay method operation complexity, inefficiency, the problem limiting the development of sugar industry.
The embodiment of the present invention is achieved in that the online test method of a kind of starch saccharification finishing point, and the online test method of this starch saccharification finishing point comprises the following steps:
Step one, measures glucose and the content of reducing sugar of saccharified liquid；
Step 2, by formula E=CG/CRCalculating, when E reaches 0.98-0.99 interval, then can determine that arrival saccharification terminal, E states saccharification terminal, CGRepresent glucose content, CRRepresent content of reducing sugar.
Further, in step one, glucose assays uses SBA-40C bio-sensing analyzer and 0.1% glucose standard, phosphate buffer.
Further, glucose assays method is: extract saccharified liquid from saccharifying tank, to be measured after dilution, taking sample after dilution with microsyringe, inject instrument reaction tank, instrument is automatically performed course of reaction, and to show that measured value, measured value are multiplied by extension rate be exactly the content of glucose in sample.
Further, in step one, reduced sugar quickly measures the employing full-automatic Iron mud of SGD-IV and 1.0% glucose solution, 1.0% methylene blue solution, Fehling solution A, Fehling second liquid.
Further, the quick method for measuring of reduced sugar is: extract saccharified liquid from saccharifying tank, to be measured after dilution, taking sample after dilution with microsyringe, inject instrument reaction tank, instrument is automatically performed course of reaction, and to show that measured value, measured value are multiplied by extension rate be exactly the content of glucose in sample.
Further, in step 2, taking saccharified liquid and be measured, with saccharificatinn period as X-axis, glucose and content of reducing sugar are Y-axis drawing, change according to glucose and concentration of reduced sugar, by calculating glucose/reduced sugar ratio, determine saccharification terminal.
The another object of the embodiment of the present invention is to provide the on-line checking automatic control system of a kind of starch saccharification finishing point, and the on-line checking automatic control system of this starch saccharification finishing point includes: main control processor, full-automatic Iron mud, bio-sensing analyzer, saccharification conditional execution unit；
Main control processor, for controlling the reduced sugar in full-automatic Iron mud and bio-sensing analyzer on-line checking saccharified liquid and the content of glucose；
Full-automatic Iron mud, is connected with main control processor, for detecting the content of the reduced sugar in saccharified liquid；
Bio-sensing analyzer, is connected with main control processor, for surveying the glucose content in saccharified liquid；
Saccharification conditional execution unit, is connected with main control processor, for determining the terminal of saccharification.
Further, main control processor utilizes controller embedded software algorithm to calculate glycosylation status the most in advance, when saccharification terminal arrives, controls saccharification and performs device.
The online test method of the starch saccharification finishing point that the present invention provides and automatic control system, by measuring glucose, the content of reduced sugar in saccharified liquid, calculate glucose and reduced sugar ratio, determining saccharification terminal, computational methods are as follows: DX/DE=glucose/reduced sugar is in conventional Mashing process, when DX/DE ratio is more than 97.5%, i.e. can be identified as saccharification terminal, in different Mashing process, can be according to actual production process control attributes, selected DX/DE control range；Immobilised enzymes glucose biological of the present invention sensing analyzer and Iron mud can carry out the mensuration of glucose and reduced sugar, it is achieved that the automation of saccharifying, unmanned and intelligent.Save manpower and materials, improve reliability and the quality of product, enhance the market competitiveness of enterprise.Additionally, the inventive method is simple, quickly, accurately, and be not required to measure dry matter content, possess the feasibility in actual production and practicality, preferably resolved existing assay method operation complexity, inefficiency, the problem limiting the development of sugar industry.
Accompanying drawing explanation
Fig. 1 is the flow chart of the online test method of the starch saccharification finishing point that the embodiment of the present invention provides；
Fig. 2 is the structural representation of the on-line checking automatic control system of the starch saccharification finishing point that the embodiment of the present invention provides；
In figure: 1, main control processor；2, full-automatic Iron mud；3, bio-sensing analyzer；4, saccharification conditional execution unit；
Fig. 3 is saccharifying reduced sugar and the glucose content detection curve figure of embodiment of the present invention offer.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Below in conjunction with the accompanying drawings and the application principle of the present invention is further described by specific embodiment.
As it is shown in figure 1, the online test method of the starch saccharification finishing point of the embodiment of the present invention comprises the following steps:
S101: quickly measure glucose and the content of reducing sugar of saccharified liquid；
S102: by formula E=CG/CRCalculate, when E reaches 0.98-0.99 interval, then can determine that arrival saccharification terminal；
In step s 102, CGRepresent glucose content, CRRepresent content of reducing sugar.
The specific embodiment of the present invention:
The first step, glucose assays
Instrument: SBA-40C bio-sensing analyzer；
Reagent: 0.1% glucose standard, phosphate buffer.
Sample: extract saccharified liquid from saccharifying tank, to be measured after dilution.
Measuring: take sample after dilution with microsyringe, inject instrument reaction tank, instrument is automatically performed course of reaction, and shows measured value.It is exactly the content of glucose in sample that measured value is multiplied by extension rate；
Second step, reduced sugar quickly measures:
The full-automatic Iron mud of instrument: SGD-IV；
Reagent: 1.0% glucose solution, 1.0% methylene blue solution, Fehling solution A, Fehling second liquid；
Sample: extract saccharified liquid from saccharifying tank, to be measured after dilution；
Measuring: take sample after dilution with microsyringe, inject instrument reaction tank, instrument is automatically performed course of reaction, and shows measured value.It is exactly the content of glucose in sample that measured value is multiplied by extension rate；
3rd step, takes saccharified liquid at regular intervals and is measured, and with saccharificatinn period as X-axis, glucose and content of reducing sugar are Y-axis drawing；In whole saccharifying, with saccharificatinn period change curve schematic diagram as shown in Figure 3, wherein curve 1 is content of reducing sugar measured value in saccharifying for reduced sugar and glucose content, and curve 2 is glucose content measured value in saccharifying, and 3 is saccharification final position；
4th step, changes according to glucose and concentration of reduced sugar, by calculating glucose/reduced sugar ratio, determines saccharification terminal.
As in figure 2 it is shown, the on-line checking automatic control system of the starch saccharification finishing point of the embodiment of the present invention is mainly made up of main control processor 1, full-automatic Iron mud 2, bio-sensing analyzer 3, saccharification conditional execution unit 4；
Main control processor 1, for controlling the reduced sugar in full-automatic Iron mud 2 and bio-sensing analyzer 3 on-line checking saccharified liquid and the content of glucose；
Full-automatic Iron mud 2, is connected with main control processor 1, for detecting the content of the reduced sugar in saccharified liquid；
Bio-sensing analyzer 3, is connected with main control processor 1, for surveying the glucose content in saccharified liquid；
Saccharification conditional execution unit 4, is connected with main control processor 1, for determining the terminal of saccharification；
Full-automatic Iron mud 2 and 3 testing results of bio-sensing analyzer are uploaded to main control processor 1 in real time, main control processor 1 utilizes controller embedded software algorithm to calculate glycosylation status the most in advance, when saccharification terminal arrives, control saccharification and perform device, stop saccharification condition to reach to meet the effect of energy-saving and emission-reduction on the premise of product requirement；
Main control processor 1 can control the various parameters of full-automatic Iron mud 2 and bio-sensing analyzer 3, controller running status, online acquisition to glucose, the concentration of reduced sugar, and real-time online shows on interpersonal interactive interface, statistical analysis, tendency chart, the function such as printing reports；
Main control processor 1:LPC1788 is the ARM Cortex-M3 microcontroller that integrated LCD image controller is released at grace intelligence Pu, it is that NXP semiconductor is for various senior communications, high quality graphic the application scenario such as show and design a there is high integration, microcontroller with Cortex-M3 as kernel, this microcontroller includes lcd controller, the Ethernet EMAC of 10/100, USB Device/Host/OTG controller at full speed, CAN controller, SPI, SSP, IIC, the resources such as IIS and external storage controller EMC, spy is applicable to the application scenario of Industry Control and medical system.
The present invention defines automatic on-line detection and FEEDBACK CONTROL closed-loop system, it is achieved that the automation of saccharifying, unmanned and intelligent.Save manpower and materials, improve reliability and the quality of product, enhance the market competitiveness of enterprise.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all any amendment, equivalent and improvement etc. made within the spirit and principles in the present invention, should be included within the scope of the present invention.
Claims (1)
1. the on-line checking automatic control system of a starch saccharification finishing point, it is characterised in that this shallow lake
The on-line checking automatic control system of pulverized sugar terminal includes: main control processor, automatically reduce
Sugar determination instrument, bio-sensing analyzer, saccharification conditional execution unit；
Described main control processor, is used for controlling full-automatic Iron mud and bio-sensing analysis
Reduced sugar in instrument on-line checking saccharified liquid and the content of glucose；
Described full-automatic Iron mud, is connected with main control processor, is used for detecting in saccharified liquid
The content of reduced sugar；
Described bio-sensing analyzer, is connected with main control processor, for surveying the grape in saccharified liquid
Sugar content；
Described saccharification conditional execution unit, is connected with main control processor, for determining the terminal of saccharification；
Main control processor utilizes controller embedded software algorithm to calculate glycosylation status the most in advance, works as sugar
When changing terminal arrival, control saccharification and perform device；
Computing formula E=C of described saccharification terminalG/CRCalculate, when E reaches 0.98-0.99 interval,
Then can determine that arrival saccharification terminal, E states saccharification terminal, and CG represents glucose content, CR
Represent content of reducing sugar.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1524965A (en) * | 2003-09-16 | 2004-09-01 | 山东省科学院生物研究所 | Rapid determination method for vitality of diastatic enzyme |
| CN102220395A (en) * | 2011-05-23 | 2011-10-19 | 北京坡华生化技术有限公司 | Biological sugaring process by using wheat starch |
| CN102253167A (en) * | 2011-04-19 | 2011-11-23 | 山东省科学院生物研究所 | Hydrolysis process based water-soluble polysaccharide analysis method |
| CN102375070A (en) * | 2010-08-27 | 2012-03-14 | 山东省科学院生物研究所 | Analytical method of starch saccharification finishing point |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1524965A (en) * | 2003-09-16 | 2004-09-01 | 山东省科学院生物研究所 | Rapid determination method for vitality of diastatic enzyme |
| CN102375070A (en) * | 2010-08-27 | 2012-03-14 | 山东省科学院生物研究所 | Analytical method of starch saccharification finishing point |
| CN102253167A (en) * | 2011-04-19 | 2011-11-23 | 山东省科学院生物研究所 | Hydrolysis process based water-soluble polysaccharide analysis method |
| CN102220395A (en) * | 2011-05-23 | 2011-10-19 | 北京坡华生化技术有限公司 | Biological sugaring process by using wheat starch |
Non-Patent Citations (1)
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| 分光光度法测定淀粉DE值;高万寿;《齐齐哈尔大学学报》;19980630(第02期);第62-65页 * |
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