CN103529159A - Method for pretreating research sample in blood non-target metabonomics - Google Patents
Method for pretreating research sample in blood non-target metabonomics Download PDFInfo
- Publication number
- CN103529159A CN103529159A CN201310491499.6A CN201310491499A CN103529159A CN 103529159 A CN103529159 A CN 103529159A CN 201310491499 A CN201310491499 A CN 201310491499A CN 103529159 A CN103529159 A CN 103529159A
- Authority
- CN
- China
- Prior art keywords
- blood
- minute
- centrifugal
- sample
- acetonitrile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004369 blood Anatomy 0.000 title claims abstract description 39
- 239000008280 blood Substances 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 36
- 238000011160 research Methods 0.000 title abstract description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims abstract description 59
- 230000004060 metabolic process Effects 0.000 claims abstract description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000000126 substance Substances 0.000 claims abstract description 11
- 239000011259 mixed solution Substances 0.000 claims abstract description 8
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 8
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 8
- 210000002966 serum Anatomy 0.000 claims abstract description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 12
- 210000002381 plasma Anatomy 0.000 claims description 12
- 239000007787 solid Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 239000010414 supernatant solution Substances 0.000 claims description 3
- 230000008859 change Effects 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 abstract description 4
- 239000002207 metabolite Substances 0.000 abstract description 4
- 238000002203 pretreatment Methods 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 239000000090 biomarker Substances 0.000 abstract description 3
- 238000001819 mass spectrum Methods 0.000 abstract description 3
- 230000007246 mechanism Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 239000011159 matrix material Substances 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract 2
- 230000004066 metabolic change Effects 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 238000001269 time-of-flight mass spectrometry Methods 0.000 abstract 1
- 238000001195 ultra high performance liquid chromatography Methods 0.000 abstract 1
- 230000002503 metabolic effect Effects 0.000 description 15
- 239000000047 product Substances 0.000 description 15
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- 239000012071 phase Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- 230000006680 metabolic alteration Effects 0.000 description 2
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 108010026552 Proteome Proteins 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 239000004047 hole gas Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000005381 potential energy Methods 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004704 ultra performance liquid chromatography Methods 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a method for pretreating a research sample in blood non-target metabonomics. Polar and non-polar substances in serum can be extracted at the same time by adopting the method. Compared with an existing common sample pretreatment method, the method has the advantages that the number of extracted metabolites is remarkably increased, protein precipitate is dissolved by acetonitrile and water mixed solution, residues dried by nitrogen is re-dissolved, so that the dissolubility of non-polar substance lysophosphatide can be reduced on the one hand, and the high mass spectrum matrix effect interference caused by high-concentration lysophosphatide can be reduced; on the other hand, the experiment operation steps are reduced, the experiment errors are reduced, and a sample treated by adopting the method can be detected by a UPLC-Q-TOF MSMS (ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry) as an analysis platform to obtain more metabolites in blood. Therefore, changes of blood metabolism can be favorably observed comprehensively, more reliable differential metabolites or biomarkers can be discovered, metabolic changes and change mechanism of a body can be further observed and researched, and the development of blood non-target metabonomics can be promoted.
Description
Technical field
The present invention relates to the sample-pretreating method of the non-target metabolism group of a kind of new blood research, a kind of a kind of new method that can simultaneously extract the sample pre-treatments of blood Semi-polarity and nonpolar metabolic product that to relate in particular to the UPLC-Q-TOF of take MSMS be analysis platform.
Background technology
Non-target plasma metabolism group is comprehensively to investigate after living things system irriate or disturbance, utilize a series of technological means and detection method to characterize the dynamic change of metabolite concentration and kind in blood, the variation physiological and pathological corresponding with body of observing the metabolic pathway of body changes.In order clearly to understand the variation of human body integral metabolism, should detect as far as possible metabolic products all in body fluid, this just needs a kind of method to extract as far as possible all metabolic products in blood.Conventional blood sample pre-treating method mainly contains now: organic solvent extraction method, solid phase extraction and both mixing methods.The cardinal principle of these methods is the protein of removing in blood, and the metabolic product extracting in blood carries out LC-MS analyzing and testing.But the weak point of this several method is the part metabolic product extracting in blood, and organic solvent extraction method is mainly apolar substance (lipid), and solid phase extraction is removed the lysophosphatide of blood middle high concentration, and the polar material in blood is extracted seldom.Therefore current sample-pretreating method can not extract polarity and the nonpolar metabolic product in blood simultaneously, can not meet the demand of all metabolic products in metabolism group analyzing blood, also just can not be comprehensive and systematic observation the plasma metabolism concrete variation and the difference that change.
Summary of the invention
Technical matters to be solved by this invention is to overcome in prior art only to pay close attention to the apolar substance that extracts in blood or the shortcoming of polar material, provides a kind of and can extract polarity in blood and the new simple and quick sample-pretreating method of apolar substance simultaneously.
In order to achieve the above object, the technology used in the present invention means are:
The pre-treating method of the non-target metabolism group of a kind of blood of the present invention study sample, is characterized in that comprising the following steps:
(1) blood of-80 ℃ of preservations is taken out, 4 ℃ thaw after vortex 1-2 minute, get and mix blood plasma 250 microlitres in 2 milliliters of centrifuge tubes, add 750 microliter methanol, vortex 1-3 minute, centrifugal 10-15 minute at 12000rpm4 ℃;
(2) the supernatant solution of getting the centrifugal rear acquisition of step (1) is in another one 2ml centrifuge tube, and nitrogen dries up, and remaining solid residue is in order to again redissolving;
(3) lower floor's albumen precipitation of the centrifugal rear acquisition of step (1) is smashed to pieces, mixed solution 350 microlitres that add acetonitrile and water, after vortex 1-3 minute, centrifugal 10-15 minute at 12000rpm4 ℃, to remove the protein in blood plasma, and extract the polar material in serum and do not extract apolar substance completely;
(4) in the centrifuge tube after the supernatant after step (3) is centrifugal is poured nitrogen in step (2) into and dried up, the remaining solid residue redissolving in centrifuge tube, standing 5-10 minute after vortex 1-3 minute, centrifugal 10-15 minute at 12000rpm4 ℃;
(5) collect the supernatant that step (4) obtains after centrifugal, be the non-target metabolism group of the blood study sample after processing.
In the present invention, preferred, in acetonitrile and water mixed solution, the volume ratio of acetonitrile and water is 1:1.
According to the sample after method processing of the present invention, can be used as the study sample of non-target plasma metabolism group, enter ULPLC/Q-TOF MSMS and detect, and then obtain data.
Compared to prior art, the pre-treating method of the non-target metabolism group of a kind of blood of the present invention study sample has the following advantages:
1, adopt method of the present invention can extract polarity and the apolar substance in serum simultaneously, compare with now conventional sample-pretreating method, the metabolic product quantity showed increased of extraction, as shown in Figure 1;
2, by again extracting polarity and the residue apolar substance in precipitating proteins, guarantee to obtain the metabolic product of maximum quantity;
3, with acetonitrile and the first soluble protein sediment of water mixed solution (preferably the volume ratio of acetonitrile and water is 1:1), residue after the nitrogen that redissolves again dries up, reduced on the one hand the solubleness of apolar substance lysophosphatide, and then the high mass spectrum matrix effect that reduction high concentration lysophosphatide causes disturbs, reduce on the other hand experimental implementation step, reduced experimental error;
4, solution, repeatedly through high speed centrifugation, has been removed the impact of the large particulate matters such as protein on chromatographic column as much as possible, without membrane filtration, can directly carry out chromatographic resolution and detection;
5, adopt the sample after method of the present invention is processed the metabolic product in more blood can be detected, more be conducive to comprehensively observe the variation of plasma metabolism, find more reliable difference metabolic product or biomarker, can further observe and study metabolic alterations and the change mechanism of body, promote the progress of the non-target metabolism group of blood.
Accompanying drawing explanation
Fig. 1 is the base peak ion current chromatogram of serum sample.
A: extracting method of the present invention; B: present conventional sample-pretreating method.
Embodiment
Below in conjunction with specific embodiment, further describe the present invention, advantage and disadvantage of the present invention will be more clear along with description.But these embodiment are only exemplary, scope of the present invention are not formed to any restriction.It will be understood by those skilled in the art that lower without departing from the spirit and scope of the present invention and can the details of technical solution of the present invention and form be modified or be replaced, but these modifications and replacement all fall within the scope of protection of the present invention.
The pre-treatment of the non-target metabolism group of embodiment 1 blood study sample
According to following steps, carry out:
(1) blood of-80 ℃ of preservations is taken out, 4 ℃ thaw after vortex 2 minutes, get and mix blood plasma 250 microlitres in 2 milliliters of centrifuge tubes, add 750 microliter methanol, vortex 2 minutes, at 12000rpm4 ℃ centrifugal 10 minutes;
(2) the supernatant solution of getting the centrifugal rear acquisition of step (1) is in another one 2ml centrifuge tube, and nitrogen dries up, and remaining solid residue is in order to again redissolving;
(3) lower floor's albumen precipitation of the centrifugal rear acquisition of step (1) is smashed to pieces, mixed solution 350 microlitres that add acetonitrile and water, in acetonitrile and water mixed solution, the volume ratio of acetonitrile and water is 1:1, after vortex 2 minutes, at 12000rpm4 ℃ centrifugal 10 minutes, to remove the protein in blood plasma, and extract the polar material in serum and do not extract apolar substance completely;
(4) in the centrifuge tube after the supernatant after step (3) is centrifugal is poured nitrogen in step (2) into and dried up, the remaining solid residue redissolving in centrifuge tube, vortex after 2 minutes standing 10 minutes, at 12000rpm4 ℃ centrifugal 10 minutes;
(5) collect the supernatant that step (4) obtains after centrifugal, be the non-target metabolism group of the blood study sample after processing.
The Mass Spectrometer Method of sample after embodiment 2 pre-treatments
1, reagent
Acetonitrile, methyl alcohol (chromatographically pure), formic acid (chromatographically pure), ultrapure water is produced by pure water instrument.
2, chromatographic separation condition:
Liquid chromatography is the U.S. Acquity of Waters company Ultra Performance Liquid Chromatography system.Chromatographic column is (BEH) C18 post (1.8 μ m, 2.1 * 100mm).Liquid phase chromatogram condition: mobile phase A is distilled water (0.1% formic acid solution), Mobile phase B is acetonitrile, flow velocity is that 0.35mL/min. is with linear gradient elution, start gradient is 2% acetonitrile and maintains 0.5min, 0.5-1.5min acetonitrile 2%-20%, 1.5-6.0min acetonitrile 20%-70%, 6.0-10.0min acetonitrile 70%-98%, 10.0-12.0min acetonitrile 98% maintains 2min, and then in 2min, balance chromatographic column 2min falls back 2%, in ethane nitrile content.Each sample size is 2 μ L, 35 ℃ of column temperatures, and auto injection actuator temperature maintains 4 ℃.
3, Mass Spectrometer Method condition:
Mass spectrometer is the Micromass Q-TOF of Waters company series connection quadrupole rod-time of-flight mass spectrometer, is furnished with electric spray ion source (ESI).Mass spectrum detects with positive ion mode, and positive ion mode condition is: capillary voltage 3000V (negative ion mode, capillary voltage is 2800V); Taper hole voltage 35V; Potential energy 6eV; Desolventizing gas velocity 650L/h; Taper hole gas velocity 50L/h; 320 ℃ of desolventizing temperature; 110 ℃ of source temperatures.Use full scan pattern, mass range is 50 – 1000amu.With the Centroid pattern of Lock Spray, collect data and guarantee accuracy and reappearance; With the LEK of 200pg/ml concentration (fixing mass-to-charge ratio: positive ion 556.2771/ negative ion 554.2615) make single point correction, flow velocity 8 μ L/min.Lock spray frequency is 0.48s, and LEK on average scans and surpasses 10 times to proofread and correct.
4, sample: the blood sample after embodiment 1 method is processed
5, result
The base peak ion current chromatogram of serum sample, as shown in Figure 1, A: extracting method of the present invention; B: present conventional sample-pretreating method, concrete steps can be referring to document:
1.Comparative?Analysis?of?Sample?Preparation?Methods?To?Handle?the?Complexity?of?the?Blood?Fluid?Metabolome:When?Less?Is?More.Sara?Tulipani,Rafael?Llorach,Mireia?Urpi-Sarda,and?Cristina?Andres-Lacueva.Anal.Chem.2013,85,341-348；
2.UPLC-MS-Based?Analysis?of?Human?Plasma?for?Metabonomics?Using?Solvent?Precipitation?or?Solid?Phase?Extraction?Filippos?Michopoulos,Lindsay?Lai,Helen?Gika,Georgios?Theodoridis,and?Ian?Wilson.Journal?of?Proteome?Research2009,8,2114–2121。
From Fig. 1 result, can find out, adopt method of the present invention to process and extract polarity and the nonpolar metabolic product in blood simultaneously, the metabolic product quantity showed increased of extracting, chromatographic peak in figure A is than the chromatographic peak showed increased in figure B, particularly increased the quantity of polar material, the chromatographic peak before retention time 0.6-5 minute significantly increases and increases.Therefore, use blood sample pre-treating method of the present invention, the metabolic product in more blood can be detected, more be conducive to comprehensively observe the variation of plasma metabolism, find more reliable difference metabolic product or biomarker, can further observe and study metabolic alterations and the change mechanism of body, thereby promote the progress of the non-target metabolism group of blood.
Claims (2)
1. a pre-treating method for the non-target metabolism group of blood study sample, is characterized in that comprising the following steps:
(1) blood of-80 ℃ of preservations is taken out, 4 ℃ thaw after vortex 1-2 minute, get and mix blood plasma 250 microlitres in 2 milliliters of centrifuge tubes, add 750 microliter methanol, vortex 1-3 minute, centrifugal 10-15 minute at 12000rpm4 ℃;
(2) the supernatant solution of getting the centrifugal rear acquisition of step (1) is in another one 2ml centrifuge tube, and nitrogen dries up, and remaining solid residue is in order to again redissolving;
(3) lower floor's albumen precipitation of the centrifugal rear acquisition of step (1) is smashed to pieces, mixed solution 350 microlitres that add acetonitrile and water, after vortex 1-3 minute, centrifugal 10-15 minute at 12000rpm4 ℃, to remove the protein in blood plasma, and extract the polar material in serum and do not extract apolar substance completely;
(4) in the centrifuge tube after the supernatant after step (3) is centrifugal is poured nitrogen in step (2) into and dried up, the remaining solid residue redissolving in centrifuge tube, standing 5-10 minute after vortex 1-3 minute, centrifugal 10-15 minute at 12000rpm4 ℃;
(5) collect the supernatant that step (4) obtains after centrifugal, be the non-target metabolism group of the blood study sample after processing.
2. the pre-treating method of the non-target metabolism group of a kind of blood according to claim 1 study sample, is characterized in that: in acetonitrile and water mixed solution, the volume ratio of acetonitrile and water is 1:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310491499.6A CN103529159B (en) | 2013-10-18 | 2013-10-18 | Method for pretreating research sample in blood non-target metabonomics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310491499.6A CN103529159B (en) | 2013-10-18 | 2013-10-18 | Method for pretreating research sample in blood non-target metabonomics |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103529159A true CN103529159A (en) | 2014-01-22 |
| CN103529159B CN103529159B (en) | 2015-05-13 |
Family
ID=49931339
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310491499.6A Active CN103529159B (en) | 2013-10-18 | 2013-10-18 | Method for pretreating research sample in blood non-target metabonomics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103529159B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424429A (en) * | 2015-11-04 | 2016-03-23 | 哈尔滨医科大学 | Sample pretreatment method for formalin fixation tissue non-target metabonomics research |
| CN109425669A (en) * | 2017-09-01 | 2019-03-05 | 中国民用航空局民用航空医学中心 | A kind of method that liquid chromatography-mass spectrometry screens degree of fatigue associated biomarkers in human body fluid |
| CN112525631A (en) * | 2020-10-16 | 2021-03-19 | 华南农业大学 | Sample preparation method for non-targeted metabonomics and non-targeted lipidomics research of shrimp meat samples |
| CN113156028A (en) * | 2021-04-30 | 2021-07-23 | 厦门市迈理奥科技有限公司 | Derivatization method of carbonyl metabolites and efficient non-targeted metabonomics analysis method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102680310A (en) * | 2012-03-20 | 2012-09-19 | 中国人民解放军第二军医大学 | Microwave assisted plasma sample pre-processing method and application in plasma metabonomics analysis |
| US20120282592A1 (en) * | 2009-08-12 | 2012-11-08 | Human Metabolome Technologies Inc. | Biomarker of depression, method for measuring biomarker of depression, computer program, and recording medium |
-
2013
- 2013-10-18 CN CN201310491499.6A patent/CN103529159B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120282592A1 (en) * | 2009-08-12 | 2012-11-08 | Human Metabolome Technologies Inc. | Biomarker of depression, method for measuring biomarker of depression, computer program, and recording medium |
| CN102680310A (en) * | 2012-03-20 | 2012-09-19 | 中国人民解放军第二军医大学 | Microwave assisted plasma sample pre-processing method and application in plasma metabonomics analysis |
Non-Patent Citations (7)
| Title |
|---|
| FILIPPOS MICHOPOULOS ET AL.: "UPLC-MS-Based Analysis of Human Plasma for Metabonomics Using Solvent Precipitation or Solid Phase Extraction", 《JOURNAL OF PROTEOME RESEARCH》, vol. 8, 27 January 2009 (2009-01-27), pages 2114 - 2121 * |
| IAN D. WILSON: "High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)-Based Drug Metabolite Profiling", 《METHODS IN MOLECULAR BIOLOGY》, vol. 708, 31 December 2011 (2011-12-31) * |
| PERRINE MASSON ET AL.: "Optimization and Evaluation of Metabolite Extraction Protocols for Untargeted Metabolic Profiling of Liver Samples by UPLC-MS", 《ANALYTICAL CHEMISTRY》 * |
| PERRINE MASSON ET AL.: "Optimization and Evaluation of Metabolite Extraction Protocols for Untargeted Metabolic Profiling of Liver Samples by UPLC-MS", 《ANALYTICAL CHEMISTRY》, vol. 82, no. 18, 17 August 2010 (2010-08-17), pages 7779 - 7786 * |
| YUAN WANG ET AL.: "Novel, fully automatic hydrophilic interaction/reversed-phase column-switching high-performance liquid chromatographic system for the complementary analysis of polar and apolar compounds in complex samples", 《JOURNAL OF CHROMATOGRAPHY A》, vol. 1204, no. 1, 11 July 2008 (2008-07-11), pages 28 - 34, XP023979622, DOI: 10.1016/j.chroma.2008.07.010 * |
| 孙蒙 等: "利用UPLC/Q-TOF MSMS对孤独症患儿血清的代谢组学分析", 《中国儿童保健杂志》, vol. 21, no. 1, 31 January 2013 (2013-01-31) * |
| 徐婧 等: "代谢组学血浆样品前处理及其快速高分辨液相色谱-质谱分析方法研究", 《分析化学》, vol. 39, no. 12, 31 December 2011 (2011-12-31), pages 1793 - 1797 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105424429A (en) * | 2015-11-04 | 2016-03-23 | 哈尔滨医科大学 | Sample pretreatment method for formalin fixation tissue non-target metabonomics research |
| CN105424429B (en) * | 2015-11-04 | 2018-03-06 | 哈尔滨医科大学 | A kind of sample-pretreating method of the non-target metabolism group research of formalin-fixed tissue |
| CN109425669A (en) * | 2017-09-01 | 2019-03-05 | 中国民用航空局民用航空医学中心 | A kind of method that liquid chromatography-mass spectrometry screens degree of fatigue associated biomarkers in human body fluid |
| CN109425669B (en) * | 2017-09-01 | 2022-09-16 | 中国民用航空局民用航空医学中心 | Method for screening biomarkers related to fatigue degree in human body fluid by liquid chromatography-mass spectrometry |
| CN112525631A (en) * | 2020-10-16 | 2021-03-19 | 华南农业大学 | Sample preparation method for non-targeted metabonomics and non-targeted lipidomics research of shrimp meat samples |
| CN113156028A (en) * | 2021-04-30 | 2021-07-23 | 厦门市迈理奥科技有限公司 | Derivatization method of carbonyl metabolites and efficient non-targeted metabonomics analysis method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103529159B (en) | 2015-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Raterink et al. | Recent developments in sample-pretreatment techniques for mass spectrometry-based metabolomics | |
| Shi et al. | Systematic profiling and comparison of the lipidomes from Panax ginseng, P. quinquefolius, and P. notoginseng by ultrahigh performance supercritical fluid chromatography/high-resolution mass spectrometry and ion mobility-derived collision cross section measurement | |
| Klepárník | Recent advances in the combination of capillary electrophoresis with mass spectrometry: From element to single‐cell analysis | |
| de Raad et al. | High-throughput platforms for metabolomics | |
| CN103308621B (en) | The method of high flux Liquid Chromatography tandem mass spectrography detection 25(OH)VD | |
| Bang et al. | Rapid and simple extraction of lipids from blood plasma and urine for liquid chromatography-tandem mass spectrometry | |
| Yi et al. | Tissue-specific metabolite profiling of alkaloids in Sinomenii Caulis using laser microdissection and liquid chromatography–quadrupole/time of flight-mass spectrometry | |
| Madji Hounoum et al. | Analytical methodology for metabolomics study of adherent mammalian cells using NMR, GC-MS and LC-HRMS | |
| Zhang et al. | Identification and characterization of the chemical constituents of Simiao Wan by ultra high performance liquid chromatography with mass spectrometry coupled to an automated multiple data processing method | |
| Li et al. | Sampling and analyte enrichment strategies for ambient mass spectrometry | |
| CN103529159B (en) | Method for pretreating research sample in blood non-target metabonomics | |
| Heydari et al. | Determination of efavirenz in plasma by dispersive liquid-liquid microextraction coupled to high-performance liquid chromatography | |
| CN103454360A (en) | Ultrafiltration and UPLC-MS/MS (ultra-high performance liquid chromatography tandem mass spectrometry) method for measuring concentration of free docetaxel in human plasma | |
| CN104215716A (en) | Method for detecting total testosterone in human serum | |
| Shao et al. | Intact living-cell electrolaunching ionization mass spectrometry for single-cell metabolomics | |
| Ye et al. | Rapid analysis of aldehydes by simultaneous microextraction and derivatization followed by GC‐MS | |
| US11808675B2 (en) | Room temperature methods for preparing biological analytes | |
| CN111141854A (en) | Method for simultaneously extracting polar and low-polar metabolites from biological sample | |
| Liu et al. | Analysis of six active components in Radix tinosporae by nonaqueous capillary electrophoresis with mass spectrometry | |
| Çelebier et al. | A foodomics approach: CE-MS for comparative metabolomics of colon cancer cells treated with dietary polyphenols | |
| Munjal et al. | Analytical techniques used in metabolomics: A review | |
| Chen et al. | Recent advancements in nanoelectrospray ionization interface and coupled devices | |
| Zhou et al. | Analysis of lignans in Magnoliae Flos by turbulent flow chromatography with online solid‐phase extraction and high‐performance liquid chromatography with tandem mass spectrometry | |
| WO2008131647A1 (en) | Detection of blood plasma schizadrin b of dissipating blood stasis botanical | |
| Dong et al. | Current mass spectrometry approaches and challenges for the bioanalysis of traditional Chinese medicines |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |