CN103529035A - Tool for researching springtails - Google Patents
Tool for researching springtails Download PDFInfo
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- CN103529035A CN103529035A CN201210227958.5A CN201210227958A CN103529035A CN 103529035 A CN103529035 A CN 103529035A CN 201210227958 A CN201210227958 A CN 201210227958A CN 103529035 A CN103529035 A CN 103529035A
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- held pole
- springtail
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- needle
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Abstract
The invention provides a tool for researching springtails, and belongs to the technical field of medical treatment and laboratory equipment. The tool comprises a needle-knife device, a springtail unfolding device, a transferring hooked needle device, a sealing sheet, a force equalizing pressing stone and a microscope. The tool for researching springtails has the characteristics that a cutting method is used for cutting a three-dimensional body of the springtail into multiple parts, and further the multiple parts are unfolded so as to form plane structures which adapt to the working principle of a microscope, thus observed classification features are clear at a glance; in a sealing process, the sizes of needle knives are suitable for the cutting of the springtail with a small body, and particularly the two needle knives can serve as a pair of scissor for cutting, so that the freewheeling cutting can be realized under the microscope; the transferring hooked needle device can be used for forming a glue drop at a hook part, so that a part to be transferred can be clearly observed and is not lost; the force equalizing pressing stone is a relatively heavy stone for uniformly applying weight to one fourth cover glass, so that the secondary three-dimensionality of a certain characteristic caused by non-uniform gravity is avoided. The tool for researching springtails is simple to operate and short in training time and has strong practicability.
Description
Technical field
The present invention relates to the instrument that a whole set of research springtail is used, belong to medical treatment and experimental facilities technical field.
Background technology
Springtail is being commonly called as of collembolan, and body size, in 1 millimeter, is active in upper soll layer, and with the three major types group of mite class, nematode formation soil animal, the kind of having reported is at present kind more than 8000.In the research process of springtail classification, known research method is after a large amount of time and efforts of cost goes to various places that springtail is gathered by the dry funnel method of Tullgren, whole health is locked in the Double layer lid slide being formed by 1 large 1 little two cover glasses by sealing liquid together, does not relate to concrete research instrument.Operation can keep the one-piece construction of springtail like this, but in actual mechanical process, because experimental tool used is such as microscopical restriction, microscopical focus is only present in some aspects, it is very fuzzy that adjacent or close architectural feature seems, after the spatial structure that adds springtail health is solid by sealing fluid-tight, can there is under the microscope refraction or the diffraction phenomena of light, even if sometimes adjust microscope, can not see object construction clearly, this situation can only be taken another springtail sample mounting again again.Even when mounting again, want more clearlyly by what see after ad hoc structure sealing, also need operating personnel's sealing technology very skilled, very skilled sealing technology needs through long training.Therefore this method demand specimen amount is large, in assorting process, many important characteristic of divisions need a plurality of samples repeatedly to verify, do not accomplish very clear, for the means of taxonomic research of springtail has increased workload greatly.In the taxonomic research process of springtail, how to accomplish to utilize a sample just can observe clearly under the microscope characteristic of divisions all on its health, reducing as far as possible the workload of springtail sort research, does is does is the credibility that increases result one and badly in need of the great technical barrier solving? so it is necessary inventing the instrument that a whole set of simple to operate, effective research springtail uses.
Summary of the invention
For solve at current method therefor, need that a large amount of samples and more feature cause because of the spatial structure of polypide and the restriction of microscopical principle of work can not open-and-shut technical barrier, the invention provides the instrument that a whole set of research springtail is used, the instrument that this research springtail is used utilizes pin cutter device that polypide is cut into a plurality of parts, then distinguish mounting, during mounting, continue cutting, make three-dimensional polypide by generate planar structure, thereby accomplish to observe at a glance the object of knowing all characteristic of divisions on this polypide on a polypide.The tool operation that research springtail is used is simple, and the training time is short, practical.
The technical solution adopted for the present invention to solve the technical problems is: the instrument used of research springtail by pin cutter device, insect extender, transfer crochet hook device, mounting, evenly defeat stone, microscope forms.Pin cutter device is with known No. 0 insect needle, with emery wheel, the tip portion of insect needle is scraped and worn into cutter shape and form point of a knife, point of a knife consists of pin cutter blade and pin cutter point of a knife two parts, then the seam of being rived from centre with cutter in the hand-held pole tip of hand-held pole front end, the insect needle that cutter shape is worn into in tip is that insert in this seam one end of the pin cutter cap of pin cutter, make hand-held pole tip clamp pin cutter, finally pin cutter is inserted to rifle head together with hand-held pole tip from rifle headgear mouth, pin cutter passes from rifle head sharp-crested, after the jam-pack rifle head of hand-held pole tip, pin cutter device is made.During scraped finish point of a knife, first look for a metallic plane, such as the surface of steel plate, scraped finish person's left hand is held insect needle, one section of insect needle tip is lain on steel plate, and the right hand is held emery wheel, firmly from insect needle cap extreme direction, scrapes to insect needle tip direction, can not scrape to insect needle cap extreme direction from insect needle tip direction, repeatedly, by insect needle conversely, from the another side just now polishing, continue scraped finish, last insect needle needle point can be polished forms cutter shape, and pin cutter can be made into.Insect extender inserts rifle head together with hand-held pole tip from rifle headgear mouth by a hair, and hair passes from rifle head sharp-crested, after the jam-pack rifle head of hand-held pole tip, with scissors, has one's hair cut and cuts off, and at rifle head sharp-crested, stays 2-3 millimeters long outward, and insect extender is made.Shift the seam of first being rived from centre with cutter in the hand-held pole tip of hand-held pole front end when crochet hook device is made, one end of the pin cap of known No. 0 insect needle is inserted in this seam, make hand-held pole tip clamp insect needle, finally insect needle is inserted to rifle head together with hand-held pole tip from rifle headgear mouth, insect needle passes from rifle head sharp-crested, after the jam-pack rifle head of hand-held pole tip, with pliers, by insect needle needle point hook, shift crochet hook device and be made.Mounting is with the label paper of rectangular Pasting, to be bonded at one end of microslide, the other end is stained with the cover glass of a 22mm * 22mm with adhesive sticker paper slip, in the central authorities of this cover glass, drip sealing liquid, both hands respectively take one pin cutter, in sealing liquid, with two pin cutter devices, do under scissor-shaped shears the each several parts such as the head of springtail health, leg, ereisma successively, polypide to be sealed or its certain part cutting down are transferred to sealing drop central authorities with shifting crochet hook device, notice that now needing to cut a part shifts a part to another mounting.During to every a part of mounting, it is whole good first with insect extender, springtail health or its certain a part of posture scaling off to be opened up under the microscope, cover gently 1/4 cover glass, with known dissecting needle flicking 1/4 cover glass, sample is locked in to 1/4 cover glass central authorities, then, with the even stone of defeating, be pressed on 1/4 cover glass, the time is not shorter than 24 hours.The even stone of defeating is by a nut with 1/4 cover glass size, with the good adhesive of commercially available taxi driver brothers, be bonded under the stone of heavily approximately 200 grams and form, this nut must the center of gravity in stone under, with guarantee stone be placed on nut after not run-off the straight or slippage, stone is the stone of picking up outdoor, and shape is according to its natural shape, if corner angle are too many, available hammer etc. is knocked out, as long as can guarantee that its weight is 200 grams of left and right.Mounting next day, must examine under a microscope the sealing situation of sample, if observe the not ideal of a certain feature sealing of after sensation, with dissecting needle, touch 1/4 cover glass, adjust a little.Then under incandescent lamp, by light baking 24-48 hour for the mounting after sealing, then with commercially available colourless nail polish, 1/4 cover glass periphery is sealed, mounting can be made into.
Beneficial effect of the present invention is, during the genealogical classification of research springtail, with pin cutter device, insect extender, transfer crochet hook device, mounting, even stone, 6 kinds of instruments of microscope of defeating, the health of springtail solid is cut into a plurality of parts, and then be made into planar structure by exhibition, adapt with microscopical principle of work, thereby accomplish that observed characteristic of division can be very clear.In cutting and mounting process, the size of pin cutter is suitable for the cutting to the less springtail of health, and particularly two pin cuttves can be done scissor-shaped shearing, can accomplish arbitrary cutting under the microscope.Opening up what use when whole is hair, and hair is flexible but also have rigidity not only, in the whole polypide of exhibition, can not cause the damage of characteristic of division.Shift crochet hook device and in hook portion, form a glue and drip, can treat the clear observation in transfer part and can not lose.The even stone of defeating is to execute equably and overweight on 1/4 cover glass with heavier stone, and a certain feature of avoiding gravity inequality to cause is again three-dimensional.
accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further described.
Fig. 1 is the pin cutter device structural representation that the present invention studies the instrument that springtail uses.
Fig. 2 is the insect extender structural representation that the present invention studies the instrument that springtail uses.
Fig. 3 is the transfer crochet hook device structural representation that the present invention studies the instrument that springtail uses.
Fig. 4 is that the present invention studies the tool needle knife structure schematic diagram that springtail is used.
Fig. 5 is that the present invention studies the tool needle nose part structural representation that springtail is used.
Fig. 6 is that the present invention studies the instrument mounting structural representation that springtail is used.
Fig. 7 is that the present invention studies the mounting structural representation that instrument that springtail uses drips sealing liquid.
Fig. 8 is the mounting structural representation that the present invention studies 1/4 cover glass in the tool cap that springtail uses.
1. hand-held poles in figure, 2. pin cutter, 3. rifle head, 4. rifle headgear mouth, 5. hand-held pole tip, 6. pin cutter cap, 7. rifle head sharp-crested, 8. hair, 9. shifts crochet hook, 10. pin cutter blade, 11. pin cutter points of a knife, 12. microslides, 13. adhesive papers, 14. cover glasses, 15. labels, 16. sealing liquid, 17.1/4 cover glass.
Embodiment
Embodiment mono-, a kind ofly studies the instrument that springtail is used, by pin cutter device, insect extender, shift crochet hook device, mounting, the even stone of defeating, microscope forms, it is characterized in that, pin cutter device is comprised of hand-held pole and point of a knife two parts, point of a knife is comprised of pin cutter blade (10) and pin cutter point of a knife (11), hand-held pole (1) front end is provided with rifle head (3), rifle head front end is provided with hand-held pole tip (5), is provided with the seam of the pin cutter cap (6) of clamping pin cutter (2), hand-held pole front end in the middle of hand-held pole tip (5), pin cutter (2), rifle head (3), hand-held pole tip (5) and pin cutter cap (6) overcoat have pin cap, and the opening that pin cap is connected in hand-held pole front end is rifle headgear mouth (4), and pin cap front end has rifle head sharp-crested (7), and pin cutter (2) passes from rifle head sharp-crested (7), shift crochet hook device by hand-held pole, insect needle, form, gap, hand-held pole tip (5) is provided with insect needle, and rifle head (3) is inserted from rifle headgear mouth (4) in insect needle and hand-held pole tip (5), and insect needle passes from rifle head sharp-crested (7), the transfer crochet hook (9) of insect needle needle point hook, mounting is by microslide (12), adhesive paper (13), cover glass (14), label (15), label (15), sealing liquid (16), 1/4 cover glass (17) forms.
Embodiment bis-, and embodiment mono-is further limited, even 180 to 220 grams of the stone weight of defeating.
Embodiment tri-, embodiment mono-is further limited, insect extender is comprised of hair (8), hand-held pole, pin cap, rifle head (3), and hair (8) inserts rifle head (3) with hand-held pole tip (5) from rifle headgear mouth (4), and hair (3) passes from rifle head sharp-crested (7).
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should be appreciated that; the present invention is not restricted to the described embodiments; that in above-described embodiment and instructions, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications; these changes and improvements all fall in the claimed scope of the invention, and the claimed scope of the present invention is defined by its equivalent of appending claims.
Claims (3)
1. study the instrument that springtail is used, by pin cutter device, insect extender, shift crochet hook device, mounting, the even stone of defeating, microscope forms, it is characterized in that, pin cutter device is comprised of hand-held pole and point of a knife two parts, point of a knife is comprised of pin cutter blade (10) and pin cutter point of a knife (11), hand-held pole (1) front end is provided with rifle head (3), rifle head front end is provided with hand-held pole tip (5), is provided with the seam of the pin cutter cap (6) of clamping pin cutter (2), hand-held pole front end in the middle of hand-held pole tip (5), pin cutter (2), rifle head (3), hand-held pole tip (5) and pin cutter cap (6) overcoat have pin cap, and the opening that pin cap is connected in hand-held pole front end is rifle headgear mouth (4), and pin cap front end has rifle head sharp-crested (7), and pin cutter (2) passes from rifle head sharp-crested (7), shift crochet hook device by hand-held pole, insect needle, form, gap, hand-held pole tip (5) is provided with insect needle, and rifle head (3) is inserted from rifle headgear mouth (4) in insect needle and hand-held pole tip (5), and insect needle passes from rifle head sharp-crested (7), the transfer crochet hook (9) of insect needle needle point hook, mounting is by microslide (12), adhesive paper (13), cover glass (14), label (15), label (15), sealing liquid (16), 1/4 cover glass (17) forms.
2. the instrument of research springtail according to claim 1, is characterized in that even 180 to 220 grams of the stone weight of defeating.
3. the instrument of research according to claim 1 springtail, it is characterized in that, insect extender is comprised of hair (8), hand-held pole, pin cap, rifle head (3), and hair (8) inserts rifle head (3) with hand-held pole tip (5) from rifle headgear mouth (4), and hair (3) passes from rifle head sharp-crested (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210227958.5A CN103529035B (en) | 2012-07-03 | 2012-07-03 | The instrument of research springtail |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210227958.5A CN103529035B (en) | 2012-07-03 | 2012-07-03 | The instrument of research springtail |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103529035A true CN103529035A (en) | 2014-01-22 |
| CN103529035B CN103529035B (en) | 2015-11-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210227958.5A Expired - Fee Related CN103529035B (en) | 2012-07-03 | 2012-07-03 | The instrument of research springtail |
Country Status (1)
| Country | Link |
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| CN (1) | CN103529035B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110376009A (en) * | 2019-08-23 | 2019-10-25 | 贵州大学 | A kind of soil mites picking tool |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0379177A1 (en) * | 1989-01-18 | 1990-07-25 | Nihon Medi-Physics Co., Ltd. | Double-sided needle assembly |
| JPH11171701A (en) * | 1997-12-06 | 1999-06-29 | Tomotake Fukuhara | Apparatus to spread the wings for specimen display |
| CN1963585A (en) * | 2006-11-21 | 2007-05-16 | 上海师范大学 | Observation apparatus for inset sample |
| JP3142294U (en) * | 2007-11-16 | 2008-06-12 | 秀基 坂田 | Straight core jig for butterfly exhibition |
| CN100517415C (en) * | 2006-05-16 | 2009-07-22 | 南京大学 | Insect extender |
| JP2010024218A (en) * | 2008-07-23 | 2010-02-04 | Ikari Shodoku Kk | Insect specimen sample set for monitoring |
| CN102461482A (en) * | 2010-11-17 | 2012-05-23 | 东北农业大学 | Springtail raising device |
-
2012
- 2012-07-03 CN CN201210227958.5A patent/CN103529035B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0379177A1 (en) * | 1989-01-18 | 1990-07-25 | Nihon Medi-Physics Co., Ltd. | Double-sided needle assembly |
| JPH11171701A (en) * | 1997-12-06 | 1999-06-29 | Tomotake Fukuhara | Apparatus to spread the wings for specimen display |
| CN100517415C (en) * | 2006-05-16 | 2009-07-22 | 南京大学 | Insect extender |
| CN1963585A (en) * | 2006-11-21 | 2007-05-16 | 上海师范大学 | Observation apparatus for inset sample |
| JP3142294U (en) * | 2007-11-16 | 2008-06-12 | 秀基 坂田 | Straight core jig for butterfly exhibition |
| JP2010024218A (en) * | 2008-07-23 | 2010-02-04 | Ikari Shodoku Kk | Insect specimen sample set for monitoring |
| CN102461482A (en) * | 2010-11-17 | 2012-05-23 | 东北农业大学 | Springtail raising device |
Non-Patent Citations (3)
| Title |
|---|
| H.SJURSEN ET AL: "Enhanced drought tolerance of a soil-dwelling springtail by preacclimation to a mild drought stress", 《JOURNAL OF INSECT PHYSIOLOGY》, vol. 47, no. 9, 30 November 2001 (2001-11-30), pages 1021 - 1027 * |
| 杨永丽: "跳虫的采集与标本制作", 《生物学通报》, vol. 43, no. 3, 31 December 2008 (2008-12-31), pages 53 - 54 * |
| 林飞荣: "昆虫标本DIY", 《教学仪器与实验》, vol. 26, no. 12, 31 December 2010 (2010-12-31), pages 48 - 50 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110376009A (en) * | 2019-08-23 | 2019-10-25 | 贵州大学 | A kind of soil mites picking tool |
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| Publication number | Publication date |
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| CN103529035B (en) | 2015-11-11 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Xie Guilin Inventor after: Pei Dianxing Inventor after: Zhao Kuijun Inventor after: Dong Shuaiwei Inventor after: Fu Rongshu Inventor after: Jiang Ming Inventor after: Xie Tianzheng Inventor after: Cheng Bo Inventor after: Ni Shifeng Inventor after: Xie Tongyin Inventor before: Wang Baozhong Inventor before: Xie Tongyin Inventor before: Pei Dianxing Inventor before: Xie Guilin Inventor before: Zhao Kuijun Inventor before: Dong Shuaiwei Inventor before: Fu Rongshu Inventor before: Jiang Ming Inventor before: Xie Tianzheng Inventor before: Cheng Bo Inventor before: Ni Shifeng |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151111 Termination date: 20160703 |
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| CF01 | Termination of patent right due to non-payment of annual fee |