CN103520285A - Veterinary honeysuckle and fructus forsythiae soluble powder and preparation method thereof - Google Patents
Veterinary honeysuckle and fructus forsythiae soluble powder and preparation method thereof Download PDFInfo
- Publication number
- CN103520285A CN103520285A CN201310447809.4A CN201310447809A CN103520285A CN 103520285 A CN103520285 A CN 103520285A CN 201310447809 A CN201310447809 A CN 201310447809A CN 103520285 A CN103520285 A CN 103520285A
- Authority
- CN
- China
- Prior art keywords
- soluble powder
- preparation
- powder
- animals
- extracting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses veterinary honeysuckle and fructus forsythiae soluble powder. The soluble powder is prepared from the following raw materials in parts by weight: 1 to 2 parts of honeysuckle and 2 to 4 parts of fructus forsythiae. The invention also discloses a preparation method of the soluble powder. The preparation method comprises the following steps: preparing crude medicines into coarse powder, adding purified water in an amount which is 8 to 10 times the weight of the coarse powder, adjusting the pH value to be 4 to 5, adding cellulase, and extracting and filtering to obtain extracting liquid and filter residue; adding purified water into the filter residue, and extracting and filtering at 40 to 50 DEG C to obtain filtrate; and combining and concentrating the extracting liquid and the filtrate to obtain concentrated liquid, adding ethanol in an amount which is 4 to 5 times the weight of the concentrated liquid, mixing uniformly, standing for 12 to 18 hours, and performing treatment to obtain the product. The method disclosed by the invention is mild in condition and simple to operate; the viscosity of the extracting liquid and the water absorption ratio of the final product are reduced the using amount of a solvent is reduced; the extracting time is shortened; and the purity of the final product is improved. The product obtained with the method contains the extracts of the honeysuckle and the fructus forsythiae, has high purity and high water solubility, and is convenient to use.
Description
Technical field
The invention belongs to pharmaceutical preparations technology for animals field, be specifically related to a kind of silver for animals and connect soluble powder and preparation method thereof.
Background technology
Plant is in growth course, and cell constantly breaks up, and the structure of cell wall, character, also in continuous change, finally can form the protection structure with certain degree of hardness.Plant cell wall is mainly to be assembled and formed by cellulosic molecule bundle, and its chief component composition is cellulose.Cellulose is that the β-D-Glucose by chain structure is formed by connecting with β-Isosorbide-5-Nitrae-glucoside bond.Ingredient in plant, as flavonoid, saponins, polysaccharide, alkaloids, volatile oil, Anthraquinones, organic acid etc., is mostly present in the cell wall of plant, only has on a small quantity and is present in intercellular substance.In complete plant cell, the extraction of effective ingredient need to be through infiltrating with infiltration, resolving and dissolve, spread and a plurality of links such as displacement, complicated operation, extract inconvenience, and extraction efficiency is low, cell wall has become the main barrier of Chinese crude drug effective component extracting.
Summary of the invention
The object of the present invention is to provide a kind of silver for animals to connect soluble powder, the preparation method of this soluble powder is provided simultaneously.
For achieving the above object, the present invention adopts following technical scheme:
Silver connects a soluble powder, and this soluble powder is made by the raw material of following weight portion: Flos Lonicerae 1-2 part, Fructus Forsythiae 2-4 part.
Above-mentioned silver for animals connects the preparation method of soluble powder, comprises the following steps:
(1) crude drug is made to coarse powder, be placed in extraction pot, add coarse powder quality 8-10 purified water doubly, regulate pH to 4-5, then add cellulase, make the cellulase that contains 8-12U in every gram of coarse powder, lixiviate 80-100min at 40-50 ℃ after stirring, filters, and obtains extracting solution and filtering residue;
(2), to adding filtering residue quality 6-8 purified water doubly in filtering residue, lixiviate 60-80min at 40-50 ℃, filters, and obtains filtrate;
(3) extracting solution and filtrate are merged, concentrated, obtain concentrated solution, add concentrated solution quality 4-5 ethanol doubly, mix, standing 12-18h obtains soluble powder by processing (processing method: get supernatant, obtain concentrated solution after recovery ethanol, concentrated solution spraying is dry).
In said method step (1), pH regulates with sulphuric acid, and after regulating, pH is 5, and extraction temperature is 50 ℃, and described cellulase adds the cellulase that contains 10U in rear every gram of coarse powder.
In said method step (2), extraction temperature is 50 ℃.
The addition of the middle ethanol of said method step (3) is 4 times of concentrated solution quality, and described time of repose is 12h; Described concentrated solution is 1.1-1.2 with respect to the density of water.
The selected medical material of the present invention has the following advantages: Flos Lonicerae is conventional Chinese medicine, has the effect of heat-clearing and toxic substances removing, wind-dispelling heat-dissipating, is mainly used in epidemic febrile disease heating, anemopyretic cold, cough due to lung-heat, laryngopharynx swelling and pain, toxic-heat and blood stasis, the treatment of the diseases such as carbuncle sore tumefacting virus; Fructus Forsythiae has the function of heat-clearing and toxic substances removing, dispersing swelling and dissipating binds, cures mainly epidemic febrile disease heating, skin ulcer general edema with yellowish skin poison.The extract of Flos Lonicerae is mainly containing take organic acid composition that chlorogenic acid is representative and glycoside, flavonoid, and Fructus Forsythiae extract master is containing take the methods of glycosides that phillyrin, Fructus Forsythiae ester glycoside be representative.
The present invention utilizes the specificity of enzyme reaction, by the composition hydrolysis of cell wall components, destroy cell wall, improved the extraction ratio of effective ingredient, the inventive method mild condition, simple to operate, reduce the viscosity of extracting solution and the water absorption rate of finished product, reduce solvent load and shorten extraction time, improving the purity of final products.
The extract that products obtained therefrom of the present invention contains Flos Lonicerae and Fructus Forsythiae, purity is high, and good water solubility is easy to use.
The specific embodiment
Below in conjunction with embodiment, the present invention is described further, but protection scope of the present invention is not limited in this.
In following embodiment, cellulase used is purchased from Shanghai Zi Yi chemical reagent work, and activity is 10,000 U/g.
embodiment 1
(1) 1 part of extracting honeysuckle, 2 parts of Fructus Forsythiaes (every part of quality is 4kg), coarse powder is made in pulverizing, be placed in extraction pot, the sulfur acid for adjusting pH that the purified water that adds 8 times of coarse powder quality is 50% by volumetric concentration is 4, then adds cellulase, make the cellulase that contains 8U in every gram of coarse powder, the lixiviate 80min at latter 40 ℃ that stirs, filters, and obtains extracting solution and filtering residue;
(2), to the purified water that adds 6 times of filtering residue quality in filtering residue, lixiviate 60min at 40 ℃, filters, and obtains filtrate;
(3) extracting solution and filtrate are merged, concentrated, the relative density of concentrated solution (with respect to the density of water) is 1.1, then adds the ethanol of 4 times of concentrated solution quality, stirring and evenly mixing, after standing 12h, get supernatant, and after reclaiming ethanol, spraying is drying to obtain even soluble powder of silver for animals.
By high performance liquid chromatography, gained silver for animals is connected to soluble powder and detect, the mass content of its Content of Chlorogenic Acid is 11.2%, and the mass content of phillyrin is 4.6%.
embodiment 2
(1) 2 parts of extracting honeysuckles, 4 parts of Fructus Forsythiaes (every part of quality is 4kg), coarse powder is made in pulverizing, be placed in extraction pot, the sulfur acid for adjusting pH that the purified water that adds 10 times of coarse powder quality is 60% by volumetric concentration is 5, then adds cellulase, make the cellulase that contains 12U in every gram of coarse powder, the lixiviate 100min at latter 50 ℃ that stirs, filters, and obtains extracting solution and filtering residue;
(2), to the purified water that adds 8 times of filtering residue quality in filtering residue, lixiviate 80min at 50 ℃, filters, and obtains filtrate;
(3) extracting solution and filtrate are merged, concentrated, the relative density of concentrated solution (with respect to the density of water) is 1.2, then adds the ethanol of 5 times of concentrated solution quality, stirring and evenly mixing, after standing 18h, get supernatant, and after reclaiming ethanol, spraying is drying to obtain even soluble powder of silver for animals.
By high performance liquid chromatography, gained silver for animals is connected to soluble powder and detect, the mass content of its Content of Chlorogenic Acid is 11.6%, and the mass content of phillyrin is 4.2%.
embodiment 3
(1) 1 part of extracting honeysuckle, 3 parts of Fructus Forsythiaes (every part of quality is 4kg), coarse powder is made in pulverizing, be placed in extraction pot, the sulfur acid for adjusting pH that the purified water that adds 9 times of coarse powder quality is 40% by volumetric concentration is 4.5, then adds cellulase, make the cellulase that contains 10U in every gram of coarse powder, the lixiviate 90min at latter 45 ℃ that stirs, filters, and obtains extracting solution and filtering residue;
(2), to the purified water that adds 7 times of filtering residue quality in filtering residue, lixiviate 70min at 45 ℃, filters, and obtains filtrate;
(3) extracting solution and filtrate are merged, concentrated, the relative density of concentrated solution (with respect to the density of water) is 1.15, then adds the ethanol of 4.5 times of concentrated solution quality, stirring and evenly mixing, after standing 16h, get supernatant, and after reclaiming ethanol, spraying is drying to obtain even soluble powder of silver for animals.
By high performance liquid chromatography, gained silver for animals is connected to soluble powder and detect, the mass content of its Content of Chlorogenic Acid is 10.9%, and the mass content of phillyrin is 4.1%.
embodiment 4
(1) 1.5 parts of extracting honeysuckles, 3.5 parts of Fructus Forsythiaes (every part of quality is 4kg), coarse powder is made in pulverizing, be placed in extraction pot, the sulfur acid for adjusting pH that the purified water that adds 9.5 times of coarse powder quality is 70% by volumetric concentration is 4.5, then adds cellulase, make the cellulase that contains 11U in every gram of coarse powder, the lixiviate 95min at latter 45 ℃ that stirs, filters, and obtains extracting solution and filtering residue;
(2), to the purified water that adds 6.5 times of filtering residue quality in filtering residue, lixiviate 75min at 45 ℃, filters, and obtains filtrate;
(3) extracting solution and filtrate are merged, concentrated, the relative density of concentrated solution (with respect to the density of water) is 1.2, then adds the ethanol of 4 times of concentrated solution quality, stirring and evenly mixing, after standing 14h, get supernatant, and after reclaiming ethanol, spraying is drying to obtain even soluble powder of silver for animals.
By high performance liquid chromatography, gained silver for animals is connected to soluble powder and detect, the mass content of its Content of Chlorogenic Acid is 12.0%, and the mass content of phillyrin is 4.7%.
Claims (5)
1. silver for animals connects a soluble powder, it is characterized in that, is made: Flos Lonicerae 1-2 part, Fructus Forsythiae 2-4 part by the raw material of following weight portion.
2. described in claim 1, silver for animals connects the preparation method of soluble powder, it is characterized in that, comprise the following steps: (1) makes coarse powder by crude drug, add coarse powder quality 8-10 purified water doubly, regulate pH to 4-5, then add cellulase, make the cellulase that contains 8-12U in every gram of coarse powder, then lixiviate 80-100min at 40-50 ℃, filters, and obtains extracting solution and filtering residue;
(2), to adding filtering residue quality 6-8 purified water doubly in filtering residue, lixiviate 60-80min at 40-50 ℃, filters, and obtains filtrate;
(3) extracting solution and filtrate are merged, concentrated, obtain concentrated solution, add concentrated solution quality 4-5 ethanol doubly, mix, standing 12-18h is by processing and obtain soluble powder.
3. as right, will go the silver-colored preparation method that connects soluble powder for animals as described in 2, it is characterized in that, in step (1), pH regulates with sulphuric acid, and after regulating, pH is 5, and extraction temperature is 50 ℃, and described cellulase adds the cellulase that contains 10U in rear every gram of coarse powder.
4. silver for animals connects the preparation method of soluble powder as claimed in claim 2, it is characterized in that, in step (2), extraction temperature is 50 ℃.
5. silver for animals connects the preparation method of soluble powder as claimed in claim 2, it is characterized in that, in step (3), the addition of ethanol is 4 times of concentrated solution quality, and described time of repose is 12h; Described concentrated solution is 1.1-1.2 with respect to the density of water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310447809.4A CN103520285A (en) | 2013-09-27 | 2013-09-27 | Veterinary honeysuckle and fructus forsythiae soluble powder and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310447809.4A CN103520285A (en) | 2013-09-27 | 2013-09-27 | Veterinary honeysuckle and fructus forsythiae soluble powder and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103520285A true CN103520285A (en) | 2014-01-22 |
Family
ID=49922872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310447809.4A Pending CN103520285A (en) | 2013-09-27 | 2013-09-27 | Veterinary honeysuckle and fructus forsythiae soluble powder and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103520285A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101759259A (en) * | 2008-10-30 | 2010-06-30 | 天津生机集团股份有限公司 | Shuanghuanglian oral liquid for aquatic product culture and a preparing method thereof |
| CN101829164A (en) * | 2010-05-07 | 2010-09-15 | 河南中医学院 | Biological preparation method of Hypericum perforatum L extractive |
| CN102225146A (en) * | 2011-05-30 | 2011-10-26 | 河南黑马动物药业有限公司 | Formula and production technology of traditional Chinese medicine injection for controlling bird flu |
| CN102526208A (en) * | 2010-12-16 | 2012-07-04 | 四川科伦药物研究有限公司 | Shuanghuanglian effervescence tablet and preparation method thereof |
| CN102885874A (en) * | 2012-10-30 | 2013-01-23 | 河南牧翔动物药业有限公司 | Preparation method of honeysuckle extract |
| CN102895317A (en) * | 2012-11-09 | 2013-01-30 | 河南牧翔动物药业有限公司 | Preparation method of Shuanghuanglian oral liquid for veterinary use |
| CN102920902A (en) * | 2011-11-03 | 2013-02-13 | 青岛康地恩药业股份有限公司 | Avian colibacillosis treatment traditional Chinese medicine and preparation method thereof |
-
2013
- 2013-09-27 CN CN201310447809.4A patent/CN103520285A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101759259A (en) * | 2008-10-30 | 2010-06-30 | 天津生机集团股份有限公司 | Shuanghuanglian oral liquid for aquatic product culture and a preparing method thereof |
| CN101829164A (en) * | 2010-05-07 | 2010-09-15 | 河南中医学院 | Biological preparation method of Hypericum perforatum L extractive |
| CN102526208A (en) * | 2010-12-16 | 2012-07-04 | 四川科伦药物研究有限公司 | Shuanghuanglian effervescence tablet and preparation method thereof |
| CN102225146A (en) * | 2011-05-30 | 2011-10-26 | 河南黑马动物药业有限公司 | Formula and production technology of traditional Chinese medicine injection for controlling bird flu |
| CN102920902A (en) * | 2011-11-03 | 2013-02-13 | 青岛康地恩药业股份有限公司 | Avian colibacillosis treatment traditional Chinese medicine and preparation method thereof |
| CN102885874A (en) * | 2012-10-30 | 2013-01-23 | 河南牧翔动物药业有限公司 | Preparation method of honeysuckle extract |
| CN102895317A (en) * | 2012-11-09 | 2013-01-30 | 河南牧翔动物药业有限公司 | Preparation method of Shuanghuanglian oral liquid for veterinary use |
Non-Patent Citations (4)
| Title |
|---|
| 旷超阳 等: "水酶法协同超声提取连翘总黄酮工艺条件优化", 《食品与发酵科技》, vol. 48, no. 01, 31 January 2012 (2012-01-31), pages 49 - 51 * |
| 旷超阳，欧阳玉祝，陈实: "水酶法协同超声提取连翘总黄酮工艺条件优化", 《FOOD AND FERMENTATION TECHNOLOGY》, vol. 48, no. 1, 31 January 2012 (2012-01-31), pages 49 - 51 * |
| 郝鹏飞，卫冰，刘富岗，杨云: "优化酶提取贯叶连翘药材活性成分工艺", 《中国实验方剂学杂志》, vol. 17, no. 14, 31 July 2011 (2011-07-31), pages 39 - 42 * |
| 马烽，朱亚玲，陈明辉，李飞: "金银花中绿原酸提取工艺研究进展", 《食品研究与开发》, vol. 31, no. 7, 31 July 2010 (2010-07-31), pages 164 - 167 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101766670B (en) | Rhodiola rosea polyphenol microcapsule and preparation method | |
| CN110240580B (en) | A method for preparing Saviae Miltiorrhizae radix extract with eutectic solvent | |
| FI72735B (en) | FOERFARANDE FOER FRAMSTAELLNING AV ANTI-INFLAMMATORISKA POLYSACKARIDER. | |
| CN109833377B (en) | Camellia oleifera Abel extract and preparation method and application thereof | |
| CN105085695A (en) | Preparation method for dendrobium candidum extract powder | |
| CN102526754A (en) | Application of gum extracted from Abelmoschus manihot as matrix of Chinese medicinal gel | |
| CN102293997B (en) | Preparation method of dried ginger extract | |
| CN110194808B (en) | Plant refined polysaccharide and application thereof in preparation of whitening cosmetics | |
| CN102626459B (en) | Method for compound enzyme-hot water extraction of cortex phellodendri alkaloids | |
| CN102885874A (en) | Preparation method of honeysuckle extract | |
| CN101229335B (en) | Enzyme method for preparing smilax scobinicaulis total saponin extract | |
| CN101955479A (en) | Method for extracting orientin from bamboo leaf | |
| CN104940280A (en) | Method for extracting total flavones from radix puerariae employing enzyme preparation | |
| CN101254217B (en) | Preparation of extract of regeneratabl portion of yew and applications of same for preparing oral anti-cancer medicine | |
| CN102875615B (en) | Extraction method and application of falcate dolichos root or leaf glucoside A and total saponins of falcate dolichos root or leaf | |
| CN111494475A (en) | Method for preparing red peony root extract | |
| CN105294793A (en) | Separation method for naringin in aizoon stonecrop herb | |
| CN103520285A (en) | Veterinary honeysuckle and fructus forsythiae soluble powder and preparation method thereof | |
| CN104761445A (en) | Method for extracting chemical components from root of mongolian medicine Cymbaria dahurica | |
| CN114657226A (en) | Method for extracting flavonoid compounds from licorice residue and utilizing extracted residue | |
| CN101450962B (en) | Method for extracting oleanolic acid from Kandelia candel leaf | |
| CN103565960B (en) | A kind of semi-bionic extraction technology prepares the method for Semen Hoveniae extract (Fructus Hoveniae extract) | |
| CN107213176B (en) | Hydrangea macrophylla leaf extract, and pharmaceutical composition, preparation method and application thereof | |
| CN102258558B (en) | Nano-sized inositol-containing cucurbita moschata duch extract, preparation method thereof, and application thereof | |
| CN101428077B (en) | Traditional Chinese medicine composition for treating diabetes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140122 |