CN103509745B - One strain Stenotrophomonas and the application in blue-green alga bloom controls thereof - Google Patents
One strain Stenotrophomonas and the application in blue-green alga bloom controls thereof Download PDFInfo
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Abstract
The invention discloses a strain Stenotrophomonas and controlling the application in blue-green alga bloom.Be separated from the water body of Taihu Lake and obtain Stenotrophomonas (Stenotrophomonas sp.) Lzh-7 that a strain has remarkable molten algae activity, preserving number is CGMCC No.6548, and separation and purification from its meta-bolites also identifies its effective molten algae composition Resorcinol, and wherein the Lethal Dose 50 LD50 of Resorcinol to microcystic aeruginosa 9110 and synechococcus BN60 is respectively 3.9 μ g/mL and 6.7 μ g/mL.Can be used for research and development and the production of new bio algicide, be finally applied to the control of lake blue algae wawter bloom.
Description
Technical field
The present invention relates to field of environment microorganism, particularly a strain has Stenotrophomonas (Stenotrophomonas sp.) Lzh-7 of molten algae activity and the application in blue-green alga bloom controls thereof.
Background technology
The outburst of blue-green alga bloom that lake eutrophication in the last few years causes is day by day serious to the pollution of lake water system, and too the freshwater such as ﹑ Dian Chi, lake ﹑ nest lake all receives serious impact, and the environment and economy problem that it causes causes the concern of people day by day.Therefore explore and control blue alga biomass and suppress the effective way of blue-green alga bloom generation to be very important.
The control techniques of blue-green alga bloom can be summarized as physical method, chemical process and biological method.Physical method such as machinery can as the subcontrol measure of algal bloom except algae etc. except algae, electromagnetic field, but shortcoming is to cure the symptoms, not the disease, and processing power is limited; Chemical process such as the chemical algicides such as weedicide directly can kill algae, but the specificity of these chemical substances is poor, and are easily enriched in food chain and cause secondary pollution.
Wherein because physical method and chemical process exist above-mentioned defect, biological method, because of its Huan Bao ﹑ economic dispatch advantage, receives increasing concern.Algae-lysing bacterium (algae-lysing bacteria) for blue-green algae is that a class can directly (contact of bacterium frustule) or indirect (the outer material of secretion born of the same parents) suppress the bacterium killing blue-green algae to be referred to as, they are important component parts of freshwater environment system, to the biomass reducing blue-green algae, maintain the eubiosis and there is vital role.
Therefore, those skilled in the art is devoted to the efficient olution-type adhesive screening efficient algae-lysing bacterium or separation and concentration algae-lysing bacterium metabolism generation, to develop microorganism algicide, in order to the control blue-green alga bloom problem of safety and efficiently.
Summary of the invention
In view of physical method in prior art with chemical process processing power is limited, the defect that easily causes secondary pollution, this technical problem to be solved finds the efficient olution-type adhesive of efficient algae-lysing bacterium and separation and concentration algae-lysing bacterium metabolism generation, in order to the control blue-green alga bloom problem of safety and efficiently.
For achieving the above object, the invention provides Stenotrophomonas and the application of meta-bolites in blue-green alga bloom controls thereof that a strain has molten algae activity.
The invention discloses Stenotrophomonas (Stenotrophomonas sp.) Lzh-7 that a strain has molten algae activity, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number CGMCC No.6548, preservation date is on September 14th, 2012.Preservation organization address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of the Chinese Academy of Sciences, postcode: 100101, phone: 86-10-64807355.
Further, the invention provides above-mentioned Stenotrophomonas Lzh-7 and control the application in blue-green alga bloom.
Further, the tunning that the invention provides above-mentioned Stenotrophomonas Lzh-7 is controlling the application in blue-green alga bloom.
Further, the invention discloses a kind of effective constituent with molten algae activity in the tunning of above-mentioned Stenotrophomonas Lzh-7, for having the compound of structure shown in structural formula (I), the invention provides the application of compound in control blue-green alga bloom that one has structure shown in structural formula (I).
Further, the invention provides a kind of molten phycomycete agent, it is characterized in that, in described molten phycomycete agent, comprise Stenotrophomonas according to claim 1 (Stenotrophomonas sp.) Lzh-7.
Further, the invention provides a kind of molten algae medicament, it is characterized in that, comprise the tunning of Stenotrophomonas according to claim 1 (Stenotrophomonas sp.) Lzh-7 in described molten algae medicament, described tunning is fermented liquid, concentrate of fermentation liquid, fermentation broth coarse extract or fermentation broth extract.
Further, the invention provides a kind of method controlling blue-green alga bloom, comprise the steps:
1), ferment Stenotrophomonas Lzh-7 bacterial strain;
2), extractive fermentation liquid;
3), extraction liquid evaporate to dryness obtains crude extract;
4), crude extract water-soluble after for controlling blue-green alga bloom.
Preferably, in step 1), fermentation condition is, Stenotrophomonas Lzh-7 is inoculated in the sterilizing beef-protein medium of pH7.0, and 28 DEG C, 200rpm envrionment conditions bottom fermentation 48h, obtain described Stenotrophomonas Lzh-7 fermented liquid.
Preferably, step 2) in, extraction agent is ethyl acetate, and in extraction system, the volume ratio of ethyl acetate and fermented liquid is 1:1, after mixing, puts into vibrator and to vibrate the extraction liquid of 24h, isolated upper strata ethyl acetate solution and Stenotrophomonas Lzh-7 fermented liquid.Fermentation broth coarse extract is after extraction liquid evaporate to dryness.Crude extract, through this area routine techniques means, as organized the method for chromatography, being purified further and can be obtained fermentation broth extract.
Described Stenotrophomonas Lzh-7 fermented liquid is respectively 4 days of following algae strain molten algae rates: chlamydomonas BS3 is 90.7 ± 11.2%, green alga B1 is 84 ± 6.1%, microcystic aeruginosa PCC7806 is 89.4 ± 3.1%, microcystic aeruginosa 9110 is 82 ± 3.2%, the algae BN35 that quivers is 84 ± 3.5%, Microcystis viridis FACHB-979 is 73.6 ± 5.7%, and synechococcus BN60 is 74.1 ± 7.4%, and chroococcoid FACHB-191 is 88.1 ± 7.9%; Within 6 days, molten algae rate is respectively: chlamydomonas BS3 is 94.1 ± 3.3%, green alga B1 is 91.2 ± 4.6%, microcystic aeruginosa PCC7806 is 95.8 ± 6.8%, microcystic aeruginosa 9110 is 99.6 ± 5.8%, the algae BN35 that quivers is 89.7 ± 11.5%, Microcystis viridis FACHB-979 is 82.3 ± 8.5%, and synechococcus BN60 is 90 ± 3.9%, and chroococcoid FACHB-191 is 99.5 ± 5.7%.
Described Stenotrophomonas Lzh-7 fermented liquid, utilize the meta-bolites of HPLC technology purifying Stenotrophomonas Lzh-7, concrete preparation method is as follows:
Use 0.22 μm of aperture membrane filtration by described extraction liquid evaporate to dryness and after being dissolved in water, filtrate is by the SupersilTM C18-EP semipreparative column of DIKMA company, and water and methyl alcohol are that the HPLC of moving phase carries out preliminary purification, obtain molten algae composition;
By described molten algae composition by DIKMA SupersilTM C18-EP analytical column, water and methyl alcohol are that the HPLC of moving phase is further purified, and obtain a kind of effective molten algae composition S-7A.
In the meta-bolites of described Stenotrophomonas Lzh-7, (IR) Jian Ce ﹑ GC-MG and NMR analyzes acquisition to the chemical structure of effective molten algae composition by infrared spectra.
When Stenotrophomonas Lzh-7 is for controlling blue-green alga bloom, the structural similitude of Resorcinol in the GC-MG analytical results of its effective molten algae composition S-7A and GC-MS database, infrared spectra (IR) detected result and organic compound spectra database (SDBS, http://riodb01.ibase.aist.go.jp/sdbs/cgi-bin/direct_frame_top.c gi) in Resorcinol corresponding, proton nmr spectra result is 1H NMR (400MHz, D2O) δ 6.84 (s, 1H), carbon-13 nmr spectra result is
13c NMR (101MHz, D2O) δ 148.94 (s), 116.36 (s).
Further, effective molten algae composition S-2A is the meta-bolites Resorcinol of described Stenotrophomonas Lzh-7.
Further, Resorcinol is 3.9 μ g/mL to the Lethal Dose 50 LD50 of microcystic aeruginosa 9110, is 6.7 μ g/mL to the Lethal Dose 50 LD50 of synechococcus BN60.
The algicidal effect of Stenotrophomonas Lzh-7 of the present invention has broad spectrum.Its fermented liquid preparation method is simple, and preparation cycle is short.The fermented liquid of Stenotrophomonas Lzh-7 has good algicidal effect to the microcystic aeruginosa 9110 in Taihu Lake, synechococcus BN60, green alga B1, the algae BN35 etc. that quivers, and microcystic aeruginosa and synechococcus are the main blue-green algaes in the blue-green alga bloom of Taihu Lake.Stenotrophomonas Lzh-7 fermented liquid and acetic acid ethyl acetate extract all can be used for the control of blue-green alga bloom or other micro-algae, and algicidal effect is better.Wherein, Stenotrophomonas Lzh-7 fermented liquid reaches 99.6 ± 5.8% to 6 of microcystic aeruginosa 9110 days molten algae rates, reaches 90 ± 3.9% to 6 days molten algae rates of synechococcus BN60.The meta-bolites Resorcinol of Stenotrophomonas Lzh-7 has good lethal effect to the main blue-green algae microcystic aeruginosa 9110 in the blue-green alga bloom of Taihu Lake and synechococcus BN60, can be used for research and development and the production of novel algicide, be finally applied to the control of lake blue algae wawter bloom.
Below with reference to accompanying drawing, to Stenotrophomonas Lzh-7 bacterial strain of the present invention and meta-bolites Resorcinol thereof, the effect in blue-green alga bloom controls is described further, to understand object of the present invention, characteristic sum effect fully.
Accompanying drawing explanation
Fig. 1 is molten phycomycete Lzh-7 to the algicidal effect of microcystic aeruginosa 9110 and synechococcus BN60;
Fig. 2 is the algicidal effect that the fermented liquid acetic acid ethyl acetate extract of molten phycomycete Lzh-7 joins on microcystic aeruginosa 9110 algae flat board;
Fig. 3 is the algicidal effect that algicidal substances Resorcinol joins on microcystic aeruginosa 9110 algae flat board;
Fig. 4 is the algicidal effect that algicidal substances Resorcinol joins to microcystic aeruginosa 9110 and synechococcus BN60 in algae liquid.
Embodiment
The screening of embodiment 1 algae-lysing bacterium
The 10mL natural water samples that Lake Taihu waters gathers is joined in the algae liquid of the microcystic aeruginosa 9110 of 90mL logarithmic phase, get yellow algae liquid gradient dilution method two days later and be coated with beef-protein medium agar plate, cultivate 24h for 28 DEG C, get the flat board that colony density is moderate, select different strains according to the difference of colonial morphology.
Inoculated respectively by the bacterial strain screened in 10mL beef-protein medium, 28 DEG C, 200rpm cultivates 24h, is added respectively by cultured for 10mL bacterium liquid in the algae liquid of 90mL logarithmic phase microcystic aeruginosa.In addition the beef-protein medium after 10mL sterilizing to be added equally in 90mL algae liquid in contrast.Calculate its molten algae efficiency after the algae liquid of all experimental group and control group cultivates 48h in illumination box, the strain bacterium that have chosen wherein molten algae efficiency high is studied further, and this bacterial strain code name is Lzh-7.
Algae liquid culture condition: microcystic aeruginosa 9110 BG11 liquid nutrient medium is cultivated, and is positioned in illumination box, 25 DEG C, intensity of illumination 40 μm of ol photons m
-2s
-1, light dark period is than being 12h:12h (illumination: dark).
Molten algae efficiency calculation method: molten algae efficiency (%)=(control group algae cell density-experimental group algae cell density)/control group algae cell density × 100.Wherein the algae cell density blood counting chamber of microcystic aeruginosa measures.
Microcystic aeruginosa 9110 cell concn curve (molten phycomycete Lzh-7 (1 × 10 over time when A is for adding molten phycomycete Lzh-7 in Fig. 1
8cells/mL) (■) and blank (◆)).Synechococcus BN60 chlorophyll-a concentration curve (molten phycomycete Lzh-7 (1 × 10 over time when B is for adding molten phycomycete Lzh-7
8cells/mL) (●) and blank (▲)).
The qualification of embodiment 2 Stenotrophomonas Lzh-7 bacterial strain
The Lzh-7 strong to algicidal effect by methods such as morphologic observation, dyeing, biochemical reactions, 16srRNA gene sequencings identifies, this bacterial strain is gram negative bacillus, and thalline is straight or slightly curved, size is 1.3x0.5 μm, single or arrange in pairs, cephalotricha, dynamic.Optimum growth temperature is 35 DEG C, obligate aerobe.Bacterium colony median size on beef-protein medium flat board, circular, yellow or brown color is not well-illuminated.Oxidase negative, DNA enzymatic is positive, and lysine decarboxylase is tested positive, and glucose oxidase decomposes slowly, and can decompose maltose by Quick Oxidation, energy liquefy gelatin, it is negative that nitrate produces nitrogen.Through 16srRNA gene sequencing and tetraploid rice, learn that in itself and GenBank, certain Stenotrophomonas bacterial strain has the homology of 99%, therefore be accredited as Stenotrophomonas and belong to bacterium, called after Stenotrophomonas Lzh-7.This bacterial classification has been preserved in national Microbiological Culture Collection management committee common micro-organisms center, and preserving number is CGMCC No.6548, and preservation date is on September 14th, 2012.Preservation organization address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of the Chinese Academy of Sciences, postcode: 100101, phone: 86-10-64807355.The accession number of 16srRNA gene in GenBank of this bacterial classification is HQ896847.
The preparation method of embodiment 3 Stenotrophomonas Lzh-7 fermented liquid and acetic acid ethyl acetate extract thereof
Be inoculated in sterilizing beef-protein medium by Stenotrophomonas Lzh-7 according to 1% inoculum size, at 28 DEG C, 200rpm shaking table obtains Stenotrophomonas Lzh-7 containing fermented liquid after cultivating 48h.Ethyl acetate is added in fermented liquid according to the ratio of 1:1, puts into vibrator and to vibrate 24h, isolate upper solution, i.e. acetic acid ethyl acetate extract.By ethyl acetate evaporate to dryness, be dissolved in water, for being further purified meta-bolites after re-using the filtering with microporous membrane in 0.22 μm of aperture.
Embodiment 4 Stenotrophomonas Lzh-7 is to the algicidal effect of different blue-green algae and Eukaryotic Algae
By 8 parts of cultured (culture condition: beef-protein medium, 28 DEG C, 200rpm cultivates 24h) 10mL Stenotrophomonas Lzh-7 bacterium liquid adds (90mL in the algae liquid of 8 strain blue-green algaes and eukaryotic algae (table 1) respectively, all algae liquid all cultivates logarithmic phase), the aseptic beef-protein medium of same use 10mL adds (90mL in 8 kinds of algae liquid respectively, all algae liquid all cultivates logarithmic phase) in contrast (1:9), the chlorophyll concentration measuring the algae liquid of all experimental group and control group after 4 days and 6 days is respectively cultivated in illumination box, calculate its molten algae efficiency.Three Duplicate Samples surveyed by each sample, are expressed as the form of mean+SD.
The algae kind used in experiment has 3 strains purchased from the aquatic institute in Wuhan algae kind storehouse, and other 5 strain screenings are from Taihu Lake water body.Experimental result shows, the fermented liquid of Stenotrophomonas Lzh-7 has good algicidal effect (table 1) for most protokaryon algae kind.This result shows that the molten algae ability of Stenotrophomonas Lzh-7 has broad spectrum, is the very potential algae-lysing bacterium of one.
Table 1. Stenotrophomonas Lzh-7 is to the algicidal effect of 8 strain algae strains
Note: screen from the Mei Liangwan water body of Wuxi, Taihu Lake with the algae strain that asterisk (*) marks in table and obtain, all the other algae strains are all buy from CHINESE FRESHWATER algae kind storehouse.
Stenotrophomonas Lzh-7 to the algicidal effect of the main blue-green algae microcystic aeruginosa 9110 in the blue-green alga bloom of Taihu Lake and synechococcus BN60 as shown in Figure 1.Figure 1A shows, after adding Stenotrophomonas Lzh-7, microcystic aeruginosa 9110 cell concn prolongation in time and declining, molten algae rate when the 6th day is 99.6%.And microcystic aeruginosa 9110 cell concn prolongation in time and rising in blank.Wherein Stenotrophomonas Lzh-7 is 1 × 10
8cells/mL.Figure 1B shows, after adding Stenotrophomonas Lzh-7, synechococcus BN60 chlorophyll-a concentration prolongation in time and declining, molten algae rate when the 6th day is 90%.And synechococcus BN60 chlorophyll-a concentration prolongation in time and rising in blank.Wherein Stenotrophomonas Lzh-7 is 1 × 10
8cells/mL.
The research of embodiment 5Lzh-7 bacterial strain algicidal mode
Stenotrophomonas Lzh-7 fermented liquid is made acetic acid ethyl acetate extract according to the method described in step 3, after centrifugal dryer evaporate to dryness, for subsequent use after dissolving with sterilized water.Finally same for sterilizing beef-protein medium centrifugal drying is concentrated in contrast.Extraction liquid obtained according to the method described above and the contrast of concentrated beef extract-peptone are added on the round scraps of paper (diameter 1cm) respectively, are positioned on BG11 solid plate that microcystic aeruginosa makes.Algae flat board is put into illumination box cultivation and within 2 days, is observed the formation of the algal control circle around the circle scraps of paper afterwards to judge its algicidal effect.
The making method of microcystic aeruginosa agar plate: pour appropriate (about 20mL) BG11 nutrient agar (agar ratio 1.5%) on every block flat board into, for subsequent use after it solidifies.Supernatant is discarded by after the centrifugal 20min of cultured for 300mL microcystic aeruginosa algae liquid 4000g, collect frustule and precipitate (1% agar in the BG11 soft agar solid medium after joining 100mL sterilizing, be positioned in 53 DEG C of water-baths and prevent from solidifying), pour the BG11 solid plate upper strata (every block flat board about pours 20mL into) made after shaking up into, put into after it solidifies illumination box cultivate and for subsequent use.
As shown in Figure 2, the fermented liquid extraction liquid of display Stenotrophomonas Lzh-7 has good algicidal effect to experimental result, and this shows that this bacterial strain has the performance that the outer algicidal substances of secretion born of the same parents carries out molten algae.
The extraction purification of molten algae effective constituent in embodiment 6 Stenotrophomonas Lzh-7 tunning
Utilize HPLC technology by the molten algae meta-bolites purifying of molten phycomycete Lzh-7, obtain a kind of purifying substance S-7A with molten algae function, concrete steps are as follows:
By the SupersilTM C18-EP semipreparative column of the extraction liquid of Stenotrophomonas Lzh-7 fermented liquid by DIKMA company, water and methyl alcohol are that the HPLC technique means of moving phase carries out preliminary purification, obtain molten algae effective constituent.Then by DIKMA SupersilTM C18-EP analytical column, water and methyl alcohol are that the HPLC technique means of moving phase is further purified, and obtain a kind of effective molten algae composition S-7A, for Structural Identification.
The Identification of chemical structure of molten algae effective constituent in embodiment 7 Stenotrophomonas Lzh-7 meta-bolites
IR ﹑ GC-MG and NMR analysis (nuclear magnetic resonance spectroscopy) is utilized to have the chemical structure of the meta-bolites of molten algae function.
Sample S-7A after purifying is carried out GC-MS analysis, with the structural similitude of Resorcinol in GC-MS database, similarity index > 850.
Sample S-7A after purifying is carried out IR analysis, and in IR detected result and organic compound spectra database (SDBS, http://riodb01.ibase.aist.go.jp/sdbs/cgi-bin/direct_frame_top.c gi), Resorcinol is corresponding.
Sample S-7A is carried out hydrogen nuclear magnetic resonance spectrum analysis, obtains following result:
1H NMR(400MHz,D2O)δ6.84(s,1H)。
Sample S-7A is carried out carbon-13 nmr spectra analysis, obtains following result:
13C NMR(101MHz,D2O)δ148.94(s),116.36(s)。
The IR ﹑ GC-MG of sample S-7A and NMR result are analyzed, can determine that the meta-bolites S-7A with algicidal effect is Resorcinol, its chemical structure is as shown below.
The molten algae effect research of embodiment 8 Stenotrophomonas Lzh-7 molten algae meta-bolites Resorcinol
Use the method in the research of above-mentioned Lzh-7 bacterial strain algicidal mode, find the BG11 solid plate that Resorcinol is made at microcystic aeruginosa can form obvious algal control circle through experiment, blank then can not form algal control circle (Fig. 3).
Resorcinol is added respectively in two kinds of blue-green algaes main in Taihu Lake and microcystic aeruginosa 9110 and synechococcus BN60, make the concentration of Resorcinol form a series respectively: 100 μ g/mL ﹑ 50 μ g/mL ﹑ 40 μ g/mL ﹑ 30 μ g/mL ﹑ 20 μ g/mL ﹑ 10 μ g/mL and 5 μ g/mL.The microcystic aeruginosa 9110 and synechococcus BN60 that add Resorcinol are put into illumination box cultivation and measure molten algae rate after 24 hours.Experimental result shows, the Lethal Dose 50 LD50 of Resorcinol to microcystic aeruginosa 9110 and synechococcus BN60 is respectively 3.9 μ g/mL and 6.7 μ g/mL (Fig. 4).This result shows that the meta-bolites Resorcinol of Stenotrophomonas Lzh-7 is the material of highly effective control blue-green algae.
Microcystic aeruginosa 9110 and synechococcus BN60 survival rate change curve (microcystic aeruginosa 9110 (◆) and synechococcus BN60 (■)) when adding different concns algicidal substances Resorcinol in Fig. 4.
The algicidal effect of Stenotrophomonas Lzh-7 of the present invention has broad spectrum.Its fermented liquid preparation method is simple, and preparation cycle is short.The fermented liquid of Stenotrophomonas Lzh-7 has good algicidal effect to the microcystic aeruginosa 9110 in Taihu Lake, synechococcus BN60, green alga B1, the algae BN35 etc. that quivers, and microcystic aeruginosa and synechococcus are the main blue-green algaes in the blue-green alga bloom of Taihu Lake.Stenotrophomonas Lzh-7 fermented liquid and acetic acid ethyl acetate extract all can be used for the control of blue-green alga bloom or other micro-algae, and algicidal effect is better.Wherein, Stenotrophomonas Lzh-7 fermented liquid reaches 99.6 ± 5.8% to 6 of microcystic aeruginosa 9110 days molten algae rates, reaches 90 ± 3.9% to 6 days molten algae rates of synechococcus BN60.The meta-bolites Resorcinol of Stenotrophomonas Lzh-7 has good lethal effect to the main blue-green algae microcystic aeruginosa 9110 in the blue-green alga bloom of Taihu Lake and synechococcus BN60, can be used for research and development and the production of novel algicide, be finally applied to the control of lake blue algae wawter bloom.
More than describe preferred embodiment of the present invention in detail.Should be appreciated that those of ordinary skill in the art just design according to the present invention can make many modifications and variations without the need to creative work.Therefore, all technician in the art, all should by the determined protection domain of claims under this invention's idea on the basis of existing technology by the available technical scheme of logical analysis, reasoning, or a limited experiment.
Claims (8)
1. a strain has Stenotrophomonas (Stenotrophomonas sp.) Lzh-7 of molten algae activity, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number CGMCC No.6548, preservation date is on September 14th, 2012, described Stenotrophomonas nitrate produces nitrogen test for negative, and the generation Resorcinol that can ferment.
2. Stenotrophomonas Lzh-7 as claimed in claim 1 is controlling the application in blue-green alga bloom.
3. the tunning of Stenotrophomonas Lzh-7 as claimed in claim 1 is controlling the application in blue-green alga bloom.
4. a molten phycomycete agent, is characterized in that, comprises Stenotrophomonas according to claim 1 (Stenotrophomonas sp.) Lzh-7 in described molten phycomycete agent.
5. a molten algae medicament, it is characterized in that, comprise the tunning of Stenotrophomonas according to claim 1 (Stenotrophomonas sp.) Lzh-7 in described molten algae medicament, described tunning is the ethyl acetate extract of fermented liquid, concentrate of fermentation liquid or fermented liquid.
6. control a method for blue-green alga bloom, comprise the steps:
1), ferment Stenotrophomonas Lzh-7 according to claim 1;
2), employing ethyl acetate is extraction agent, extractive fermentation liquid;
3), extraction liquid evaporate to dryness obtains crude extract;
4), crude extract water-soluble after for controlling blue-green alga bloom.
7. method as claimed in claim 6, wherein step 1) in, fermentation condition is, Stenotrophomonas Lzh-7 is inoculated in the sterilizing beef-protein medium of pH 7.0,28 DEG C, 200rpm envrionment conditions bottom fermentation 48h, obtain described Stenotrophomonas Lzh-7 fermented liquid.
8. method, wherein step 2 as claimed in claim 6) in, the volume ratio of ethyl acetate and fermented liquid is 1:1.
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| 溶藻细菌对水华与赤潮微藻的抑制效应研究;汪辉;《中国优秀博士学位论文数据库》;20120615;摘要，第51页第1段、第58页第6段 * |
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