CN103504287A - Method for extracting honey fungus mycelium based on membrane technology - Google Patents
Method for extracting honey fungus mycelium based on membrane technology Download PDFInfo
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- 239000012528 membrane Substances 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 35
- 238000005516 engineering process Methods 0.000 title claims abstract description 23
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- 238000001471 micro-filtration Methods 0.000 claims abstract description 31
- 238000000855 fermentation Methods 0.000 claims abstract description 23
- 230000004151 fermentation Effects 0.000 claims abstract description 23
- 239000012530 fluid Substances 0.000 claims description 24
- 235000012907 honey Nutrition 0.000 claims description 19
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- 238000001035 drying Methods 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 7
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- 230000004913 activation Effects 0.000 claims description 4
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- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 3
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- 235000009508 confectionery Nutrition 0.000 claims 1
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- 238000000926 separation method Methods 0.000 abstract description 11
- 230000008901 benefit Effects 0.000 abstract description 10
- 230000006378 damage Effects 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 1
- 229910052760 oxygen Inorganic materials 0.000 abstract 1
- 239000001301 oxygen Substances 0.000 abstract 1
- 238000004065 wastewater treatment Methods 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
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- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
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- 230000008929 regeneration Effects 0.000 description 4
- 238000011069 regeneration method Methods 0.000 description 4
- 235000011121 sodium hydroxide Nutrition 0.000 description 4
- 238000010561 standard procedure Methods 0.000 description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 3
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- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
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- 229930186147 Cephalosporin Natural products 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000305492 Gastrodia Species 0.000 description 1
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- QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
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- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 206010039897 Sedation Diseases 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 239000002269 analeptic agent Substances 0.000 description 1
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- 238000005265 energy consumption Methods 0.000 description 1
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- 229910001410 inorganic ion Inorganic materials 0.000 description 1
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- 239000007788 liquid Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
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- 230000007935 neutral effect Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 229960002275 pentobarbital sodium Drugs 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000036280 sedation Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Jellies, Jams, And Syrups (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for extracting honey fungus mycelium based on a membrane technology. In the method, a honey fungus fermentation liquor is processed and separated through a microfiltration membrane to obtain the honey fungus mycelium. The method changes the traditional centrifuge method for separating honey fungus, adopts an efficient membrane separation technology to perform mycelium separation, reduces mycelium entering the filtrate, improves the yield of mycelium, reduces COD (chemical oxygen demand) of the filtrate, reduces the wastewater treatment capacity without damage to the cells, and ensures that the effective components in the cells are completely recovered. Compared with the traditional technology, the method is of obvious economic benefit, social benefit and environment-protecting benefit.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of mycelial method of extraction fungi fermentation, more precisely a kind of new method of extracting fermentation halimasch filament based on membrane technology.
Background technology
Halimasch powder (Gastrodia Tuder Halimasch Powder) Shi Bai mushroom section fungi halimasch [Armillaria mellea (Vahl.ex.Fr.) Quel.] is cultivated through deep fermentation, through refining the forming of separation and Extraction, is dried bacterium powder.Halimasch is a kind of fungi of dietotherapeutic, under natural surroundings with rhizoma Gastrodiae symbiosis.Institute of Materia Medica,Chinese Academy of Medical Sciences has carried out pharmacological testing, and result proof halimasch fermentate and rhizoma Gastrodiae have similar pharmacological action.(1) sedation: have the effect of strengthening pentobarbital sodium sub-threshold lull dosage, can extend the length of one's sleep of mouse, obviously reduce the freely movable of mouse; (2) anticonvulsant action: central nervous system stimulant amylene tetrazolium is had to certain antagonism; (3) strengthen the effect of the resistance to anoxic power of mouse; (4) effect of enhanced machine body immunity function; (5) other effects: can reduce the mouse death rate that nicotine causes, Mouse Whole Brain ischemic is had to cerebral protection, can increase cerebral blood flow (CBF) and the coronary blood flow of dog
Compare with natural rhizoma Gastrodiae, halimasch powder has following several large features: 1, similar in chemical composition and pharmacological action; 2, halimasch powder toxicity is lower than natural rhizoma Gastrodiae; 3, halimasch powder price is well below natural rhizoma Gastrodiae, and can produce in a large number.Based on above-mentioned particular advantages, halimasch pruinescence is widely used in pharmacy, health products trade.
Tradition honey ring separating technology adopts horizontal screw centrifuge to carry out separation more, which is because being limited by the impact of centrifuge separation factor, in separation process, mycelium suffers shearing force destruction, part active ingredient is lost in filtrate and causes product quality and yield to decline, separating obtained filtrate is more muddy, more broken mycelia is contained in the inside, cause outer row's liquor C OD higher, if this filtrate reaches sewage drainage standard, need to pass through complicated sewage disposal, sewage disposal system workload Tai great ，Gei enterprise has brought serious financial burden.Some Enterprises, in order to reclaim as far as possible the residual mycelium in filtrate, is taked to add halimasch filament to after concentrate drying to filtrate, and because amount of filtrate is large, drying time is long, not only wastes mass energy, and product quality is caused and had a strong impact on.
Summary of the invention
The object of the invention is in order to solve the deficiency of above-mentioned honey ring separating and extracting process, thereby designed a kind of novel environment-friendly process based on membrane technology separation and Extraction honey ring zymotic fluid, this technique can improve mycelial yield, reduce the mycelium that enters filtrate, reduce the COD of filtrate, reduce sewage load; Do not destroy again cell itself, guarantee whole recovery of active ingredient in born of the same parents.This invention has not only improved yield and the active ingredient of halimasch filament, has improved product quality, has increased economic benefit; Reduce again COD in waste water, alleviated sewage disposal system pressure, increased social benefit.
Object of the present invention mainly realizes according to following step:
A kind of method based on membrane technology extraction halimasch filament is to carry out according to following processing step:
1) get residual sugar and be less than 0.5, reduced sugar is lower than 0.3, and the honey ring zymotic fluid of fermentation broth viscosity 100～150cP, by microfiltration membranes, obtains micro-filtration dislysate and the mycelium of zymotic fluid, collects and obtains mycelium; Microfiltration membranes aperture is at 0.05～1 μ m, and operating temperature is 10～70 ℃, operating pressure: entrance pressure is 2～8bar, and going out to press is 0～5bar;
2) cryogenic vacuum oven dry: by step 2), the mycelium of gained is-0.065～-0.095Mpa in vacuum, under 70～90 ℃ of conditions, is dried, and the dry thing of gained is halimasch filament.
The preferred microfiltration membranes of the method aperture is at 0.05～0.5 μ m, and operating temperature is 50～60 ℃, operating pressure: entrance pressure is 2～4bar, and going out to press is 1～2bar.Microfiltration membranes is ceramic membrane or organic film, and its molecular cut off is 8000-100000Da.
Be 30～35 hours described drying time, and the dry thing water content obtaining is 3～8%.
Zymotic fluid described in the method prepares by the following method: preparation honey ring seed liquor after halimasch strain activation, the seed liquor preparing is inoculated into fermentation medium, inoculum concentration 5%～8%, fermentation medium consists of glucose 2%, soybean cake powder 3%, sucrose 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.03%, defoamer 0.1%, water preparation, pH value nature, it is dense thick that zymotic fluid finishes rear detection zymotic fluid, pH value drops to 5.0 left and right, after zymotic fluid finishes, obtain zymotic fluid pH value and drop to 4.9～5.1, zymotic fluid residual sugar is less than 0.5, reduced sugar is lower than 0.3.
Microfiltration membranes used in the present invention is renewable, and 1-2% nitric acid and 1-2% liquid caustic soda for regeneration obtain by soda-wash water and acid washing water neutralization the neutral water that contains inorganic ion, and this water is as fermentation batching water.
The present invention is applicable to any Armillaria mellea, and as being deposited at the Armillaria mellea Armillaria of Chinese agriculture microorganism fungus kind preservation administrative center, the caterpillar fungus cephalosporin bacterial classification that deposit number ACCC50063 etc. can freely spare all can be used.
The present invention provides a kind of new method for honey ring separation and Extraction.The method has advantages of following outstanding:
1, improve yield: microfiltration membranes aperture 0.05-1 μ m, has significantly improved mycelia yield.
2, improve the quality of products: in traditional horizontal screw centrifuge separation process, halimasch filament suffers shearing force destruction, main effectively component portion is lost in filtrate and causes mycelium active ingredient to reduce, this process using micro-filtrate membrane filtration, in operating process, shearing force can be do not produced, mycelium active ingredient can be effectively guaranteed; Compared with filtrate concentrate drying recovery process, compare, this technique obtains that product index is stable, smell color and luster homogeneous.
3, reduce filter liquor COD, alleviate environmental protection pressure: the sleeping spiral shell separation of tradition is because mycelium rejection is low, and part mycelium enters filtrate, causes COD increased content in filtrate, this method separation factor is high, mycelium rejection is increased greatly, filtrate clarification, in filtrate, mycelium is few, the dislysate COD obtaining reduces greatly, its COD index can reach direct discharge standard, and only sewage disposal Jiu Wei every day enterprise reduces by 30 tons for the treatment of capacities, greatly reduces sewage disposal expense.
4, clean production continuously, energy consumption is low: the inventive method can be produced continuously, film and corollary equipment long service life thereof, convenience for device maintenance, small investment, operating process noise-less pollution, easy to clean.
In a word, the present invention adopts suitable honey ring zymotic fluid in conjunction with the membrane material in suitable aperture, can extract to greatest extent the halimasch filament in zymotic fluid, adopt membrane technology not only to improve product yield, yield is up to 61g/L, product quality also has very large lifting, and wherein polyoses content is with DEXTROSE ANHYDROUS (C
6h
12o
6) meter be up to 7.5%, peptide content is up to 8.1%, compared with conventional method, has improved respectively 1 and 1.7 percentage, according to WS-10001-(HD-1224)-2002 standard method, checks; With aperture 0.05 μ m film, calculate, the membrane filtration gained mycelium before centrifugal yield of sleeping spiral shell improves 11g/L, tank that put every day batch increases halimasch filament 500Kg(siccative meter) more than, separating obtained filtrate is limpid bright, COD content reduces greatly, alleviate environmental protection pressure ，Gei enterprise and brought significant economic benefit, social benefit and environmental benefit.
The specific embodiment
By specific embodiment, further illustrate the present invention below.But the detail of embodiment only, for explaining the present invention, should not be construed as limited overall technical solution.
Embodiment 1
1) halimasch bacterial strain (Armillaria) provides and identifies, deposit number ACCC50063 by Chinese agriculture microorganism fungus kind preservation administrative center.By preparation honey ring seed liquor after the conventional activation of bacterial strain, bacterial strain 2～3 rings that oese is got activation are inoculated into seed culture medium, cultivate about 7-8 days shaking speed 120r/min for 26～28 ℃, seed culture medium forms glucose 3%, dusty yeast 2%, peptone 1.5%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.03%, defoamer 0.1%, water preparation, pH value nature.The seed liquor preparing is inoculated into fermentation medium, inoculum concentration 5%～8%, and fermentation medium consists of glucose 2%, soybean cake powder 3%, sucrose 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.03%, defoamer 0.1%, water preparation, pH value nature.Zymotic fluid finishes rear detection zymotic fluid and slightly turns rare by thick, and pH value drops to 5.0 left and right, and microscopy part mycelia starts self-dissolving, and fluorescent weakens, and without living contaminants, zymotic fluid residual sugar is less than 0.5, and reduced sugar is lower than 0.3.
2) micro-filtration filters: the 5000Kg zymotic fluid after the fermentation of halimasch kind (halimasch filament give money as a gift content be 7.5%) is filtered by micro-filtration, obtain honey ring wet mycelium 1154kg(water content 74%), micro-filtration dislysate 3846Kg, the light transmittance of dislysate is 80%, COD is 65mg/L, can directly discharge.It is that aperture is the ceramic membrane of 50nm that micro-filtration filters membrane material used, and concrete technology condition is: operating temperature is 50-60 ℃, and entrance pressure is 3.0bar, goes out to press for 1.0bar, and pressure differential is 2.0bar.Microfiltration membranes regeneration: by concentration, be that 1% aqueous solution of nitric acid and 1% sodium hydrate aqueous solution rinse microfiltration membranes, in soda acid wash water and after squeeze into fermentation material-compound tank, then add corresponding culture medium and on a small quantity inorganic salts as fermentation medium.
3) low temperature drying: the 1154kg honey ring wet mycelium that step 2 is obtained enters double cone dryer dries, obtain the halimasch filament of 304.5kg moisture 1.48%, this mycelium polysaccharides 7.5%, peptide content 8.1%, checks according to WS-10001-(HD-1224)-2002 standard method.Concrete technology condition is: baking temperature 75-85 ℃, vacuum is-0.085Mpa, drying time 31h.
Embodiment 2
1) micro-filtration filters: the 5000Kg zymotic fluid after the halimasch kind fermentation preparing according to embodiment 1 method (halimasch filament give money as a gift content be 7.5%) is filtered by micro-filtration, obtain honey ring wet mycelium 1130kg(water content 75%), micro-filtration dislysate 3870Kg, the light transmittance of dislysate is 80%, COD is 65mg/L, can directly discharge.It is that aperture is the ceramic membrane of 100nm that micro-filtration filters membrane material used, and concrete technology condition is: operating temperature is 50-60 ℃, and entrance pressure is 4.0bar, goes out to press for 2.0bar, and pressure differential is 2.0bar.Microfiltration membranes regeneration: by concentration, be that 1% aqueous solution of nitric acid and 1% sodium hydrate aqueous solution rinse microfiltration membranes, in soda acid wash water and after squeeze into fermentation material-compound tank, then add corresponding culture medium and on a small quantity inorganic salts as fermentation medium.
2) low temperature drying: the 1130kg honey ring wet mycelium that step 2 is obtained enters double cone dryer dries, obtain the halimasch filament of 288.5kg moisture 2.1%, this mycelium polysaccharides 7.3%, peptide content 7.9%, according to WS-10001-(HD-1224)-2002 standard method checks.Concrete technology condition is: 85～90 ℃ of baking temperatures, vacuum is-0.075Mpa, drying time 30h.
Embodiment 3
1) micro-filtration filters: the 5000Kg zymotic fluid after the halimasch kind fermentation preparing according to embodiment 1 method (halimasch filament give money as a gift content be 7.5%) is filtered by micro-filtration, obtain honey ring wet mycelium 1169kg(water content 74%), micro-filtration dislysate 3831Kg, the light transmittance of dislysate is 80%, COD is 65mg/L, can directly discharge.It is that aperture is the ceramic membrane of 50nm that micro-filtration filters membrane material used, and concrete technology condition is: operating temperature is 30-40 ℃, and entrance pressure is 8.0bar, goes out to press for 3.0bar, and pressure differential is 5.0bar.Microfiltration membranes regeneration: by concentration, be that 1% aqueous solution of nitric acid and 1% sodium hydrate aqueous solution rinse microfiltration membranes, in soda acid wash water and after squeeze into fermentation material-compound tank, then add corresponding culture medium and on a small quantity inorganic salts as fermentation medium.
2) low temperature drying: the 1169kg honey ring wet mycelium that step 2 is obtained enters double cone dryer dries, obtain the halimasch filament of 308kg moisture 1.9%, this mycelium polysaccharides 7.2%, mannitol content 7.8%, according to WS-10001-(HD-1224)-2002 standard method checks.Concrete technology condition is: 75～80 ℃ of baking temperatures, vacuum is-0.085Mpa, drying time 32.5h.
Claims (5)
1. based on membrane technology, extract a method for halimasch filament, it is characterized in that the method comprises the steps:
1) get residual sugar and be less than 0.5, reduced sugar is lower than 0.3, and the honey ring zymotic fluid of fermentation broth viscosity 100～150cP, by microfiltration membranes, obtains micro-filtration dislysate and the mycelium of zymotic fluid, collects and obtains mycelium; Microfiltration membranes aperture is at 0.05～1 μ m, and operating temperature is 10～70 ℃, operating pressure: entrance pressure is 2～8bar, and going out to press is 0～5bar;
2) cryogenic vacuum oven dry: by step 2), the mycelium of gained is-0.065～-0.095Mpa in vacuum, under 70～90 ℃ of conditions, is dried, and the dry thing of gained is halimasch filament.
2. the method for extracting honey ring based on membrane technology according to claim 1, is characterized in that microfiltration membranes aperture described in the method is at 0.05～0.5 μ m, and operating temperature is 50～60 ℃, operating pressure: entrance pressure is 2～4bar, and going out to press is 1～2bar.
3. the method for extracting honey ring based on membrane technology according to claim 1, is characterized in that the microfiltration membranes in the step 1 described in the method is ceramic membrane or organic film.
4. the method for extracting honey ring based on membrane technology according to claim 1, is characterized in that be 30～35 hours described drying time, and the dry thing water content obtaining is 1～3%.
5. according to claim 1 based on the sweet method of encircling of membrane technology extraction, it is characterized in that the zymotic fluid described in the method prepares by the following method: preparation honey ring seed liquor after halimasch strain activation, the seed liquor preparing is inoculated into fermentation medium, inoculum concentration 5%～8%, fermentation medium consists of glucose 2%, soybean cake powder 3%, sucrose 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.03%, defoamer 0.1%, water preparation, pH value nature, zymotic fluid finishes rear detection zymotic fluid and slightly turns rare by thick, pH value drops to 5.0 left and right, after fermentation ends, obtain zymotic fluid pH value and drop to 4.9～5.1, zymotic fluid residual sugar is less than 0.5, reduced sugar is lower than 0.3.
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| CN201310441259.5A CN103504287A (en) | 2013-09-25 | 2013-09-25 | Method for extracting honey fungus mycelium based on membrane technology |
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| CN201310441259.5A CN103504287A (en) | 2013-09-25 | 2013-09-25 | Method for extracting honey fungus mycelium based on membrane technology |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109370916A (en) * | 2018-12-04 | 2019-02-22 | 昆明理工大学 | A kind of process for rapid activation of halimasch aging strain |
| CN115873718A (en) * | 2022-10-25 | 2023-03-31 | 江苏神华药业有限公司 | Liquid fermentation medium of armillaria mellea mycelium, and preparation method and application thereof |
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2013
- 2013-09-25 CN CN201310441259.5A patent/CN103504287A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109370916A (en) * | 2018-12-04 | 2019-02-22 | 昆明理工大学 | A kind of process for rapid activation of halimasch aging strain |
| CN115873718A (en) * | 2022-10-25 | 2023-03-31 | 江苏神华药业有限公司 | Liquid fermentation medium of armillaria mellea mycelium, and preparation method and application thereof |
| CN115873718B (en) * | 2022-10-25 | 2024-08-16 | 江苏神华药业有限公司 | Liquid fermentation medium for armillaria mellea mycelium and preparation method and application thereof |
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