CN103503792A - Method for training detection bees and letting detection bees fly - Google Patents
Method for training detection bees and letting detection bees fly Download PDFInfo
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- CN103503792A CN103503792A CN201310472477.5A CN201310472477A CN103503792A CN 103503792 A CN103503792 A CN 103503792A CN 201310472477 A CN201310472477 A CN 201310472477A CN 103503792 A CN103503792 A CN 103503792A
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Abstract
The invention discloses a method for training detection bees and letting the detection bees fly. The conditioned stimulus (target smell) time, the conditioned stimulus (target smell) intensity, the unconditioned stimulus (syrup awarding) time and the unconditioned stimulus (syrup awarding) intensity of the bees are trained, partial bees in one swarm are trained every day and are trained to memorize a target smell; bees which master and memorize the target smell are put back to the original swarm, the target smell is placed in the position a certain distance away from the swarm, and the frequency of the observed phenomenon that the swarm of bees visit the target smell every day is recorded through a camera every day; the training intensity required for training one swarm of bees with respect to a certain target smell is estimated according to the date when the highest visiting frequency of the bees happens and the total of the bees put back at the same time, the most applicable number of days for searching the target smell by a trained swarm is determined according to the number of days when the bees visit the target smell at high frequency, and finally swarms are trained and let fly according to the training intensity and the best flying opportunity to achieve the purpose of searching suspicious targets in a region.
Description
Technical field
The invention belongs to animal behavior inductive technology field, specifically relate to a kind of method of training and let fly away search bee.
Background technology
Training and let fly away honeybee can help people to search and search the risk objects such as the explosive in specific region, violated chemicals.As far back as 1999, the researcher of U.S. Los Alamos National Laboratories (Los Alamos National Laboratory) just starts to train honeybee for searching the feasibility of explosive, scientific research personnel's formal training honeybee to 2007 Nian， Zagreb, CRO universities (Zagreb University) is used for clearance.The method that they trained and let fly away honeybee is: a bee colony is placed in a net canopy, in net canopy, place some saucers, in plate, fill the syrup that some contain explosive, allow honeybee take food these syrup, after a period of time, the part honeybee meeting of ging to school remember the smell of explosive in bee colony, then moves bee colony to region to be checked, allows it search explosive.Researcher thinks that to utilize feeding method training a army of bees more many than a honeybee difficulty of training, and apply above-mentioned training and let a army of bees fly away and have following problem: while 1) feeding, will often move net canopy, generally at night, carry out, also will constantly convert the position of placing syrup in net canopy; 2) conventionally feed 1 day, then hungry 1 day, then feed 1 day, adopt and feed and hungry over-over mode, encourage honeybee study and identification explosive smell; 3), because limiting in net canopy, just minority honeybee takes food repeatedly, the chance that in bee colony, most honeybees cannot fully be learnt and train, and training strength is limited; 4) be unable to estimate and let fly away the best opportunity that bee colony finds target.In a word, the method is a qualitative method, and the efficiency of training is low, and how undesirable the effect of training is also, up to the present, utilizes the method training and let honeybee fly away that to find the successful case of risk object also fewer.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, a kind of method that proposes new training and let search bee fly away, by controlling stimulation time and the intensity of target smell and award syrup, improve the precision of training honeybee, and can quantize the intensity for the required training bee colony of certain target smell, the best period that bee colony finds target is let in judgement fly away.
Object of the present invention is achieved by the following technical programs.
Training and a method of letting investigation honeybee fly away, comprise the steps:
(1) on honeycomb doorway, tackle out 15～20 of nest workers bee, put it in vial, in each vial, put into 1～2, and with being with foraminate lid to cover tightly vial; The frozen water that adds 0 ℃ in container, then inserts vial wherein, after 8～10 minutes, takes out honeybee numb with cold, and constraint honeybee health makes it only have the freely activity of head and feeler;
(2) after the honeybee having fettered revives, with syringe needle, beat the feeler of honeybee, inducing bees is stretched kiss, then advances syringe, overflows syrup, allows honeybee suck, until have enough and regain their kiss; The honeybee of fed to appetite is put into carton box, cover lid, open in dark place; After 2 hours, from box, take out honeybee, with the feeler that the syringe needle of syringe beats honeybee, detect honeybee and whether stretch kiss, if stretch kiss, show that this honeybee has good respond, can be used for training; 10 honeybees of each selection are for training;
(3) target smell training: wherein 1 honeybee is placed in training environment, then first familiarization training environment 40 seconds opens the valve of carrying target smell, by the speed of 33 milliliters/second, supplies gas 5 seconds; After supplying gas the 3rd second, opens the valve of carrying syrup, overflows syrup 2 microlitres, allows honeybee suck in 3 seconds; Allow afterwards honeybee in training environment, stop 15 seconds, complete once training; According to remaining honeybee of same operation training, until 10 honeybees are all accepted a target smell training;
(4) blank air-flow training: be only not contain target smell in air-flow with the difference of step (3), all the other operations are identical, complete once training; According to remaining honeybee of same operation training, until 10 honeybees are all accepted the training of primary blank air-flow;
(5) repeating step (3) and (4), allow every honeybee accept 3 target smell training and 3 blank air-flow training; 10 honeybees that complete alternately training are placed in to new training house, beat the feeler of honeybee with syringe needle, inducing bees is stretched kiss, then advances syringe, overflows syrup, allows honeybee suck syrup, until have enough and regain their kiss; Subsequently, the honeybee of fed to appetite is put into carton box, cover lid, be put into dark place;
After (6) 2 hours, in carton box, take out honeybee, with the syringe needle of syringe, beat the feeler of honeybee and whether stretch kiss to detect honeybee; Leave the honeybee that stretches kiss, with these honeybees of target Odor stimulation, and observe it and occurring under target Odor stimulation, whether stretching kiss, with blank air-flow, stimulate these honeybees again and determine whether it stretches kiss, if target Odor stimulation is stretched kiss and blank air-flow is stimulated and do not stretch kiss, show that these honeybees have learned and remembered this smell, mark association remember the honeybee of target smell then, and they are put back to original bee colony.
Further, when training and putting and return honeybee, every day is at the local drop target smell apart from 50 meters, bee colony, and near target smell, follows the tracks of, records with video camera the frequency that the honeybee of training is visited target smell; According to honeybee visit target in bee colony, there is the time of high frequency time and now put returning honeybee total, estimate training number of days and training honeybee number for certain target training bee colony, according to time and the continuous days of training honeybee high frequency visit target smell, determine the best opportunity of letting the bee colony of undergoing training fly away.
With respect to prior art, the present invention has the following advantages:
1, the present invention fully controls stimulation time and the intensity of training honeybee, improved the precision of training honeybee, also quantized for a necessary training strength of bee colony of certain target smell training, and can accurately estimate to utilize a trained bee colony to find the best period of target.
2, the present invention completes the training to a bee colony under laboratory condition, can avoid constantly carrying bee colony, net canopy and mobile food, time saving and energy saving, and training process is not affected by weather and place etc.
Accompanying drawing explanation
Fig. 1 training strength and honeybee searching target frequency graph of a relation.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail, but drawings and Examples are not limited to the technical solution.
Embodiment 1
1. select a army of bees, on honeycomb doorway, only tackle out nest worker bee 15-20, put into vial (each vial is only generally only put into 1-2), with being with foraminate lid to cover tightly vial.In the beaker of 250 milliliters, add the cold water of 150 milliliters, and in cold water, put into some ice cubes, in then vial being inserted, allow honeybee body temperature decline gradually, after about 10 minutes, honeybee starts numb with cold, take out honeybee, they are bundled on the metallic support of training honeybee special use.In the process of binding, honeybee health is all in bond, only has head and the freely activity of feeler of honeybee.
2. wait that the honeybee having fettered comes to life, beat the feeler of honeybee with the syringe needle of 1 milliliter of syringe, inducing bees is stretched kiss, then advances syringe, overflows syrup, allows honeybee suck syrup, until honeybee has enough and regains their kiss.The honeybee of fed to appetite is put into carton box, cover lid, open in dark place, after 2 hours, take out honeybee, whether the feeler that beats honeybee with the syringe needle of 1 milliliter of syringe is normal to detect honeybee.If honeybee stretches kiss, show that this honeybee has good respond, can be used for training, reject the honeybee that does not stretch kiss.10 of each selections are trained.
3. first wherein 1 honeybee is put on training station, allow its familiarization training platform 40 seconds, open the valve of carrying acetic anhydride smell, by the speed of 33 milliliters/second, supply gas 5 seconds, and in latter 3 seconds of the beginning of supplying gas, open filling system and the transport openings of carrying syrup, overflow syrup 2 microlitres, allow honeybee in 3 seconds, suck (control smell and syrup delivery amount and speed can by computer control), then allow honeybee stop 15 seconds at training station, train 1 honeybee 1 minute consuming time altogether.Afterwards, train after the same method all the other 9 honeybees, until 10 honeybees are all accepted the training of a target smell.
4. when all honeybees are all after the training of a target smell, repeat 3. process, speed and the time of supplying gas are constant, just in air-flow, do not contain acetic anhydride smell, allow 10 honeybees all accept once only to have the stimulation of blank air-flow, this process is in order to eliminate the mechanical stimulating action of air-flow to honeybee.
5. repeat subsequently 3. and 4., allow every honeybee accept the alternately training of 3 acetic anhydride Odor stimulations and 3 blank air-flow stimulations, having completed the training of 10 honeybees.Until 10 3 acetic anhydride smells of honeybees acceptance and 3 blank air-flows, replace after training, with the syringe needle of 1 milliliter of syringe, beat honeybee feeler, inducing bees is stretched kiss, then advance syringe, overflow syrup, allow honeybee suck syrup, until honeybee has enough and regains kiss.Subsequently, the honeybee of fed to appetite is put into carton box, cover lid, open in dark place.
6. after 2 hours, in carton box, take out honeybee, with the syringe needle of 1 milliliter of syringe, beaing honeybee feeler whether normal to detect it if stretching kiss.Leave the honeybee that stretches kiss, then with acetic anhydride, stimulate these honeybees, and observe it and whether stretch kiss, with blank air-flow, stimulate honeybee to observe it again and whether stretch kiss, if kiss is stretched in the stimulation of Dichlorodiphenyl Acetate acid anhydride, blank air-flow is stimulated and do not stretch kiss, show that this honeybee learned and remembered acetic anhydride, the honeybee of acetic anhydride smell is also remembered, and put back to its original bee colony by mark association.
Training and a method of letting search bee fly away, comprise the steps:
The first step: according to according to 1.-6. step, train every day to part honeybee in bee colony, puts the honeybee of learn and remember acetic anhydride smell to return original bee colony, training every day and put and return about 50 honeybees, lasting 30 days of training;
Second step: when training and putting and return, in the place apart from 50 meters, bee colony, place a little spitball (table tennis size) that soaks peracetic acid acid anhydride every day, and set up a video camera in the place of 3 meters of the little spitballs of distance, for the honeybee visit spitball number of times of whole day record training bee colony;
The 3rd step: according to continuous training in 30 days, put and return and observed and recorded, the high frequency time (15 times/hour) that the little spitball of peracetic acid acid anhydride is soaked in the visit of this group of honeybee appears at the 21st day after training, now put that to return the quantity of honeybee be 257, honeybee high frequency is visited little spitball and has been continued altogether the time (21-23 days after training) of 3 days, infer thus for acetic anhydride target, at least to train 300 honeybees, if train 30 every day, at least train 20 days, the most suitable time of utilizing training honeybee to find the acetic anhydride odor source in region only has 3 days (shown in Fig. 1).
The 4th step: train some groups of honeybees, every group of training are let fly away after 20 days and found region internal object for 3 days.
More than show and described the advantage of basic principle of the present invention and principal character and invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (2)
1. a method of training and letting fly away search bee, comprises the steps:
(1) on honeycomb doorway, tackle out 15～20 of nest workers bee, put it in vial, in each vial, put into 1～2, and with being with foraminate lid to cover tightly vial; The frozen water that adds 0 ℃ in container, then inserts vial wherein, after 8～10 minutes, takes out honeybee numb with cold, and constraint honeybee health makes it only have the freely activity of head and feeler;
(2) after the honeybee having fettered revives, with syringe needle, beat the feeler of honeybee, inducing bees is stretched kiss, then advances syringe, overflows syrup, allows honeybee suck, until have enough and regain their kiss; The honeybee of fed to appetite is put into carton box, cover lid, open in dark place; After 2 hours, from box, take out honeybee, with the feeler that the syringe needle of syringe beats honeybee, detect honeybee and whether stretch kiss, if stretch kiss, show that this honeybee has good respond, can be used for training; 10 honeybees of each selection are for training;
(3) target smell training: wherein 1 honeybee is placed in training environment, then first familiarization training environment 40 seconds opens the valve of carrying target smell, by the speed of 33 milliliters/second, supplies gas 5 seconds; After supplying gas the 3rd second, opens the valve of carrying syrup, overflows syrup 2 microlitres, allows honeybee suck in 3 seconds; Allow afterwards honeybee in training environment, stop 15 seconds, complete once training; According to remaining honeybee of same operation training, until 10 honeybees are all accepted a target smell training;
(4) blank air-flow training: be only not contain target smell in air-flow with the difference of step (3), all the other operations are identical, complete once training; According to remaining honeybee of same operation training, until 10 honeybees are all accepted the training of primary blank air-flow;
(5) repeating step (3) and (4), allow every honeybee accept 3 target smell training and 3 blank air-flow training; 10 honeybees that complete alternately training are placed in to new training house, beat the feeler of honeybee with syringe needle, inducing bees is stretched kiss, then advances syringe, overflows syrup, allows honeybee suck syrup, until have enough and regain their kiss; Subsequently, the honeybee of fed to appetite is put into carton box, cover lid, be put into dark place;
After (6) 2 hours, in carton box, take out honeybee, with the syringe needle of syringe, beat the feeler of honeybee and whether stretch kiss to detect honeybee; Leave the honeybee that stretches kiss, with these honeybees of target Odor stimulation, and observe it and occurring under target Odor stimulation, whether stretching kiss, with blank air-flow, stimulate these honeybees again and determine whether it stretches kiss, if target Odor stimulation is stretched kiss and blank air-flow is stimulated and do not stretch kiss, show that these honeybees have learned and remembered this smell, mark association remember the honeybee of target smell then, and they are put back to original bee colony.
Training according to claim 1 and let fly away investigation honeybee method, it is characterized in that: when training and putting and return honeybee, every day is at the local drop target smell apart from 50 meters, bee colony, and with video camera, follows the tracks of, records near target smell the frequency of the honeybee visit target smell of training; According to honeybee visit target in bee colony, there is the time of high frequency time and now put returning honeybee total, estimate training number of days and training honeybee number for certain target training bee colony, according to time and the continuous days of training honeybee high frequency visit target smell, determine the best opportunity of letting the bee colony of undergoing training fly away.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310472477.5A CN103503792B (en) | 2013-10-11 | 2013-10-11 | Method for training scout bees and letting scout bees fly |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310472477.5A CN103503792B (en) | 2013-10-11 | 2013-10-11 | Method for training scout bees and letting scout bees fly |
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| CN103503792A true CN103503792A (en) | 2014-01-15 |
| CN103503792B CN103503792B (en) | 2015-06-03 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104825497A (en) * | 2015-03-28 | 2015-08-12 | 郭云胶 | Antiallergic bee venom health care wine and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101606510A (en) * | 2009-07-21 | 2009-12-23 | 中国科学院昆明动物研究所 | Olfaction-conditioning training device for fixed bees |
| CN101606511A (en) * | 2009-07-21 | 2009-12-23 | 中国科学院昆明动物研究所 | Training method for odor discrimination based on flapping reflex of bees to electric shock |
| CN202354203U (en) * | 2011-11-29 | 2012-08-01 | 中国科学院西双版纳热带植物园 | Automatic training device for bee colony |
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2013
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101606510A (en) * | 2009-07-21 | 2009-12-23 | 中国科学院昆明动物研究所 | Olfaction-conditioning training device for fixed bees |
| CN101606511A (en) * | 2009-07-21 | 2009-12-23 | 中国科学院昆明动物研究所 | Training method for odor discrimination based on flapping reflex of bees to electric shock |
| CN202354203U (en) * | 2011-11-29 | 2012-08-01 | 中国科学院西双版纳热带植物园 | Automatic training device for bee colony |
Non-Patent Citations (2)
| Title |
|---|
| H.E洛巴舍夫 等: "利用视觉条件刺激进行蜜蜂的训练", 《中蜂养蜂》 * |
| 一亜佛 等: "为大面积农作物授粉的新的蜜蜂训练法", 《中国养蜂》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104825497A (en) * | 2015-03-28 | 2015-08-12 | 郭云胶 | Antiallergic bee venom health care wine and preparation method and application thereof |
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