CN103503779B - New pure white hypsizigus marmoreus strain - Google Patents
New pure white hypsizigus marmoreus strain Download PDFInfo
- Publication number
- CN103503779B CN103503779B CN201310030559.4A CN201310030559A CN103503779B CN 103503779 B CN103503779 B CN 103503779B CN 201310030559 A CN201310030559 A CN 201310030559A CN 103503779 B CN103503779 B CN 103503779B
- Authority
- CN
- China
- Prior art keywords
- finc
- mushroom
- pure white
- true
- bacterial strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Mushroom Cultivation (AREA)
Abstract
The invention provides a pure white hypsizigus marmoreus strain Finc-W-227 of which the collection number is CCTCC NO:M2012377. The pure white hypsizigus marmoreus strain Finc-W-227 has the advantages of low probability of occurrence of cap mushroom tumors, and high single yield, and is suitable for large-area popularization and planting.
Description
[technical field]
The present invention relates to edible mushroom technical field, more precisely, relate to the true Ji's mushroom bacterial strain of a kind of pure white.
[background technology]
True Ji mushroom (Latin title Hypsizygus marmoreus), being subordinate to Basidiomycotina, Hymenomycetes, Agaricales, Bai Mo section, belonging to from gill fungus race, beautiful gill fungus, because it has unique crab delicate flavour, therefore is hypsizygus marmoreus also known as it.Initial artificial cultivation started from Japan before and after 1978, and its delicious flavour, sense of food are tender and crisp, nutritious, and its taste is fresher than flat mushroom, and its meat is thicker than sliding mushroom, and its matter is more tough than mushroom, and mouthfeel is excellent, and its dry product also thick flavor overflows.True Ji mushroom contains enriches vitamin and 17 seed amino acids, and β-1, the 3-D glucan extracted in fruit body has very high antitumor activity, is a kind of low in calories, low-fat health food.
True Ji mushroom is from cultivation beginning, and what substantially walk is exactly the route of factorial praluction.Because factory culture has anniversary continuous seepage, large-scale production, mostly realize semi-mechanization, mechanization so that intellectuality, growing environment controls better, and yield and quality has had good guarantee.As everyone knows, the factory culture of bacterial classification to edible mushroom plays vital effect.Current true Ji mushroom has dark brown and the main cultivation strain of pure white two, and pure white strain, also known as " white beech mushroom ", " Yulong mushroom ", is received by the market, and main breed only has No. 1, the Big Dipper white beech mushroom (hereinafter referred to as " H-W ") of Japanese Big Dipper company.H-W is the pure white true Ji mushroom strain that seed selection in 2002 is released, and its entire body is snow-white, without bitter taste, add the aesthetic feeling of dish, but it still exists following defect: knurl lid mushroom easily appears in (1), and yields is low; (2) per unit area yield is lower, affects economic benefit.
[summary of the invention]
The technical problem that the present invention solves is the defect overcoming prior art existence, provides a kind of per unit area yield high, and pure white true Ji's mushroom bacterial strain Finc-W-227 that knurl lid mushroom occurrence rate is low.
The present invention is achieved by the following technical solutions, and pure white of the present invention true Ji's mushroom bacterial strain Finc-W-227 is after being hybridized by parent TNN-11 and H-W, then obtains through systematic breeding.This bacterial strain is preserved in China typical culture collection center, address on September 24th, 2012: Wuhan University of Wuhan, China city, and its preserving number is CCTCC NO:M 2012377, its biological classification called after Hypsizygus marmoreus Finc-W-227.
The feature of pure white of the present invention true Ji's mushroom bacterial strain Finc-W-227 is:
1, morphological feature
The mycelia of Finc-W-227: pure white sparse, aerial hyphae is undeveloped; Cap: section is in circle mountain type, and entire body is snow-white, and meat is thick, meat is crisp, and speckle is clear, central distribution, without be full of cracks, not rounding; Lamella: snow-white, wavy bending, sparse; Without spore print; Stem: thick, snow-white, without hair, raw with the slightly biased heart of cap, meat is crisp.
2, biological property
Nutrient component source is: corncob, weed tree sawdust, rice bran, wheat bran, corn flour, cotton seed hulls etc., wherein, and water content 64 ~ 66%.Mycelium culture temperature 20 ~ 25 DEG C, humidity 75%, CO2 concentration 2500 ~ 4000ppm, cultivation cycle 70 ~ 90d.Suitable fruiting temperature is 13 ~ 15 DEG C, humidity 93 ~ 100%, CO2 concentration 1000 ~ 2000ppm, front 1 ~ 7d intensity of illumination 50 ~ 100Lux, rear 8 ~ 20d intensity of illumination 200 ~ 300Lux, and every day, clearance-type opened layer bracket lamp stimulation fruiting, cultivation period 24 ~ 26d.
Compared with prior art, pure white of the present invention true Ji's mushroom bacterial strain Finc-W-227 significantly reduces knurl lid mushroom probability of occurrence, improves single rate simultaneously, suits large area to popularize.
[accompanying drawing explanation]
Fig. 1 is that Finc-W-227 and H-W per unit area yield, cultivation period are with cell age change curve.
Fig. 2 is that Finc-W-227 and H-W mycelial growth rate varies with temperature curve map.
Fig. 3 is the pcr amplification electrophoresis pattern of primer I TS1 and ITS4 to 4 bacterial strains, and wherein 1 is bacterium H-W, and 2 is Finc-W-227, and 3 is G-W, and 4 is GC-W, M is 10kbp DNA ladder.
Fig. 4 is Finc-W-227 phylogenetic tree.
Fig. 5 is the pcr amplification electrophoresis pattern of primer S99 to 4 bacterial strains, numbers same Fig. 3.
[embodiment]
Described " G-W " bacterial strain in following examples is the true Ji's mushroom bacterial strain of one commercially, and its former base is grey, and along with the g and D of fruit body bleaches gradually, picking time, stem was canescence, and cap is pure white; Described " GC-W " bacterial strain is the white beech mushroom bacterial strain that Japanese Ge Cheng is newly bred as.
Embodiment 1 hybridizes acquisition Finc-W-227
The breeding process of pure white of the present invention true Ji's mushroom bacterial strain Finc-W-227 comprises the steps:
1) Selection parent bacterial strain: choosing TNN-11 and H-W is parent, the fruit body of these two parents all commercially can be bought and obtain, and in Academy of Agricultural Sciences, Shanghai City, also there is preservation in DSMZ.
2) medium is prepared
PDA medium: every 1L medium potato 200g, glucose 20g, agar 20g.
Pedigree seed culture medium: wood chip 65% (percentage by weight, lower same), rice bran 18%, wheat bran 7%, corn flour 10%, water content is 60% ~ 62%.
Cultivar culture medium: corncob 21%, weed tree sawdust 15%, rice bran 20%, wheat bran 20%, corn flour 5%, cotton seed hulls 19%, wherein water content 64% ~ 66%.
3) conventional method obtains heterozygote 800.
4) cultivation screening: the heterozygote obtained is carried out experiment in cultivation and screening according to the method for routine.
5) aimed strain is obtained: by screening system, from 800 heterozygotes, obtain a pure white bacterial strain, its production capacity is high, improves productivity effect, reaches breeding goal.This bacterial strain is named as " Finc-W-227 ".
Pure white of the present invention true Ji's mushroom bacterial strain Finc-W-227 is preserved in China typical culture collection center on September 24th, 2012, and deposit number is CCTCC NO:M 2012377, and bacterial strain is survived after testing, and classification position is that beautiful gill fungus belongs to true Ji mushroom.
Embodiment 2 cell age is tested
The present embodiment is by carrying out cultivation cycle (i.e. cell age to planting H-W the most widely in pure white of the present invention true Ji mushroom bacterial strain Finc-W-227 and the true Ji mushroom of existing pure white, lower with), the contrast of cultivation period and per unit area yield, advantage and the feature of pure white of the present invention true Ji's mushroom bacterial strain Finc-W-227 are described.
The Cultivar culture medium that the present embodiment adopts is: corncob 35%, weed tree sawdust 14%, rice bran 14%, wheat bran 19%, corn flour 5%, cotton seed hulls 13%, wherein water content 64% ~ 66%.
The cell age test of table 1 Finc-W-227 and H-W
Take H-W as contrast, arrange 8 cell ages from 70d (i.e. sky, lower same) to 105d, often increasing 5d is a cell age, every cell age 80 bottles.Cultivate and cultivate and adopt conventional true Ji mushroom to cultivate and cultivation parameter, the volume of culture bottle is 850cc, bottleneck diameter 58mm.
This result of the test sees also Fig. 1 and table 1, and the highest per unit area yield of H-W is 161g/bottle (namely gram/bottle, lower with), best cell age is 95d, and corresponding cultivation period is 23d, and the highest per unit area yield of Finc-W-227 reaches 228g/bottle, best cell age is 90d, and cultivation period is 25d.Known by contrasting, advantage is clearly in single rate for Finc-W-227, when cell age is in the scope of 70d ~ 90d, the cultivation period of Finc-W-227 is at about 25d, the cultivation period of 75d and 80d two cell age Finc-W-227 shortens than H-W, and the cultivation period of the Finc-W-227 of 85d and 90d two cell ages is slightly longer than H-W.Described in comprehensive, in 70d ~ 105d cell age, the single rate of Finc-W-227 improves 30.6% ~ 45.2% than H-W.
The fresh-keeping test of embodiment 3
Taking H-W as contrast, after Finc-W-227 and H-W gathers, packing by often wrapping 155 ~ 165g, each 20 bags, in freezer (3 DEG C ~ 8 DEG C) preservation 11d, then take out under being placed on 23 DEG C of room temperature conditions, record Fruitbody situation of change.Result of the test refers to table 2, and after freezer (3 DEG C ~ 8 DEG C) preservation 11d, it is still intact that Finc-W-227 continues preservation 11d at 23 DEG C of environment; The 10d that H-W continues preservation at 23 DEG C of environment starts, by snow-white flavescence, to lose feeling of freshness, and Finc-W-227 13d part under identical environment starts to turn yellow; H-W is after freezer preservation 11d, and 23 DEG C of 18d continuing preservation all lose commercial value, and Finc-W-227 just all loses commercial value after continuing preservation 20d at 23 DEG C.More than illustrate that the freshness date of Finc-W-227 is than H-W slightly advantage.
The fresh-keeping test of table 2 Finc-W-227 and H-W
Embodiment 4 mycelial growth temperature gradient test
The inoculation block being 5.0mm Finc-W-227 and H-W diameter is inoculated on PDA medium, and cultivate 11d respectively at 20 DEG C, 25 DEG C, 30 DEG C and 35 DEG C, result of the test is in table 7 and Fig. 2.Result of the test shows: the mycelia of Finc-W-227 is very fast 20 DEG C ~ 25 DEG C growth rate, and visible, its suitable cultivation temperature is 20 ~ 25 DEG C.
The mycelial growth temperature gradient test of table 3 Finc-W-227 and H-W
Embodiment 5 morphological feature and biological property
Morphological feature: Finc-W-227 mycelia is pure white sparse, and aerial hyphae is undeveloped; Cap section circle mountain type, entire body is snow-white, and meat is thick, meat is crisp, and speckle is clear, central distribution, without be full of cracks, not rounding; Lamella is snow-white, wavy bending, sparse; Without spore print; Stem is thick, snow-white, without hair, raw with the slightly biased heart of cap, meat is crisp.The difference of the concrete form feature of Finc-W-227 and H-W refers to table 4.
Table 4 Finc-W-227 and H-W morphological feature contrasts
Biological property: nutrient component source is: corncob, weed tree sawdust, rice bran, wheat bran, corn flour and cotton seed hulls etc., wherein, water content 64% ~ 66%.Mycelium culture temperature 20 ~ 25 DEG C, humidity 75%, CO
2concentration 2500ppm ~ 4000ppm, cultivation cycle 70d ~ 90d.Suitable fruiting temperature is 13 ~ 15 DEG C, humidity 93% ~ 100%, CO
2concentration 1000ppm ~ 2000ppm, front 1d ~ 7d intensity of illumination 50Lux ~ 100Lux, rear 8d ~ 20d intensity of illumination 200Lux ~ 300Lux, every day, clearance-type opened layer bracket lamp stimulation fruiting, cultivation period 24d ~ 26d.
Embodiment 6 antagonistic effect
The present embodiment is with the true Ji's mushroom bacterial strain of pure white of the present invention Finc-W-227 and H-W, G-W, GC-W for test material, and used medium is PDA medium.
Finc-W-227 and H-W, G-W, GC-W be face-off inoculation and cultivation respectively on plating medium, and vaccination is at a distance of 2cm, 24 DEG C of constant temperature culture 26d.
Antagonistic effect result is with reference to table 5, at the front and back of opposite culture bacterium colony intersection, Finc-W-227 and H-W, Finc-W-227 and G-W and Finc-W-227 and GC-W antagonism line is obvious, illustrates that Finc-W-227 is the true Ji mushroom bacterial strain different from H-W, G-W and GC-W.
Antagonistic effect between table 5 different true Ji mushroom
Embodiment 7ITS checks order qualification
In the present embodiment, ITS order-checking student on commission work biotechnology (Shanghai) limited company (hereinafter referred to as " raw work ") carries out, and comprises following process:
Genomic extraction: extract according to raw work SK1375 fungal genomic DNA extraction agent box specification.Obtained DNA solution is placed in-20 DEG C, refrigerates for subsequent use.
Pcr amplification and Establishing (25 μ L): genomic templates 1 μ L, fungi rDNA universal primer ITS15'-TCCGTAGGTGAACCTGCGG-3'(10 μM) 0.5 μ L, ITS45'-TCCTCCGCTTATTGATATGC-3'(10 μM) 0.5 μ L, dNTP mix (10mM each) 0.5 μ L, 10 × Taq reaction buffer 2.5 μ L, Taq (5u/ μ L) 0.2 μ L, adds water to 25 μ L.PCR program: denaturation 94 DEG C of 5mim; 94 DEG C of 30s, 55 DEG C of 35s, 72 DEG C of 1min, 35 circulations, 72 DEG C extend 8min.The PCR primer that amplification obtains is by agarose gel electrophoresis, and its electrophoresis result is shown in Fig. 3.
DNA Ago-Gel reclaims glue purification and order-checking adopts conventional method.
The ITS sequence sequencing result of this test strain Finc-W-227 is shown in sequence table, and its sequence is the DNA fragmentation of 665bp.Check in from GenBank, the ITS sequence similitude that Finc-W-227 bacterial strain ITS sequence and beautiful gill fungus belong to true Ji mushroom is the highest.And from GenBank, checking in the ITS sequence belonging to the close kind of true Ji mushroom with beautiful gill fungus, application DNAStar MegAlign software building goes out the phylogenetic tree of strains tested, refers to Fig. 4.
Embodiment 8RAPD analyzes
The present embodiment for analytic target, utilizes RAPD analytical technology with the true Ji's mushroom bacterial strain of pure white of the present invention Finc-W-227 and H-W, G-W, GC-W, further the hereditary capacity of the true Ji's mushroom bacterial strain of qualification pure white of the present invention.In the present embodiment, DNA extraction, pcr amplification and agarose gel electrophoresis are identical with embodiment 7, do not repeat them here.
Refer to table 6, during RAPD analyzes, from 12 random primers, filter out 1 primer can distinguish Finc-W-227 and H-W, the true Ji's mushroom strains of G-W, GC-W clearly, this random primer is S99.
Table 6 random primer
The result that RAPD analyzes is as follows:
The pcr amplification agarose gel electrophoretogram that primer S99 is corresponding is the position at Fig. 5, a frame place, and Finc-W-227 is without DNA band, and H-W, G-W and GC-W have DNA band, Finc-W-227 and H-W is described, G-W and GC-W is different bacterial strains.More than illustrate that primer S99 can distinguish Finc-W-227 and H-W, G-W and GC-W bacterial strain, Finc-W-227 is true Ji mushroom new strains.
More than describe and be only embodiments of the invention, forgive and can understand, under the prerequisite not departing from the present invention's design, all should be included within technical conceive of the present invention simple modification of the present invention and replacement.
Claims (1)
1. pure white true Ji's mushroom bacterial strain Finc-W-227, its deposit number is CCTCCNO:M 2012377.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310030559.4A CN103503779B (en) | 2013-01-25 | 2013-01-25 | New pure white hypsizigus marmoreus strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310030559.4A CN103503779B (en) | 2013-01-25 | 2013-01-25 | New pure white hypsizigus marmoreus strain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103503779A CN103503779A (en) | 2014-01-15 |
| CN103503779B true CN103503779B (en) | 2015-07-22 |
Family
ID=49887638
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310030559.4A Active CN103503779B (en) | 2013-01-25 | 2013-01-25 | New pure white hypsizigus marmoreus strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103503779B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105165635B (en) * | 2015-10-22 | 2017-05-24 | 青岛丰科生物科技有限公司 | Hypsizygus marmoreus strain and application thereof |
| CN106434381B (en) * | 2016-10-31 | 2018-12-21 | 上海丰科生物科技股份有限公司 | Pure white true pleurotus cornucopiae bacterial strain and its molecular labeling, specific primer to and application |
| CN113637591B (en) * | 2020-04-27 | 2023-10-03 | 西充星河生物科技有限公司 | Hypsizygus marmoreus strain |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060041984P1 (en) * | 2004-08-19 | 2006-02-23 | Mizue Yamazaki | Bunashimeji mushroom plant named "HOKUTO-18" |
| CN101608213A (en) * | 2008-06-17 | 2009-12-23 | 上海市农业科学院 | A kind of discrimination method of hypsizigus marmoreus mating type gene |
| CN101642054A (en) * | 2009-09-04 | 2010-02-10 | 福建农林大学 | Hypsizigus marmoreus and method for establishing laccase transfer system in breeding thereof |
| JP2011223995A (en) * | 2010-03-31 | 2011-11-10 | Takara Bio Inc | Buna shimeji mushroom strain and method for producing buna shimeji mushroom fruit body |
| CN102884939A (en) * | 2011-07-22 | 2013-01-23 | 上海丰科生物科技股份有限公司 | Cultivation method of hypsizygus marmoreus |
-
2013
- 2013-01-25 CN CN201310030559.4A patent/CN103503779B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060041984P1 (en) * | 2004-08-19 | 2006-02-23 | Mizue Yamazaki | Bunashimeji mushroom plant named "HOKUTO-18" |
| CN101608213A (en) * | 2008-06-17 | 2009-12-23 | 上海市农业科学院 | A kind of discrimination method of hypsizigus marmoreus mating type gene |
| CN101642054A (en) * | 2009-09-04 | 2010-02-10 | 福建农林大学 | Hypsizigus marmoreus and method for establishing laccase transfer system in breeding thereof |
| JP2011223995A (en) * | 2010-03-31 | 2011-11-10 | Takara Bio Inc | Buna shimeji mushroom strain and method for producing buna shimeji mushroom fruit body |
| CN102884939A (en) * | 2011-07-22 | 2013-01-23 | 上海丰科生物科技股份有限公司 | Cultivation method of hypsizygus marmoreus |
Non-Patent Citations (2)
| Title |
|---|
| 刘明广等.真姬菇杂交菌株H11的选育.《食用菌》.2008,第17-19页. * |
| 真姬菇不同菌株的筛选试验;范书华;《中国林副特产》;20080228(第1期);第35-36页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103503779A (en) | 2014-01-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103330258B (en) | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof | |
| CN103477993B (en) | Pure white new hypsizigus marmoreus bacterial strain | |
| CN105165635B (en) | Hypsizygus marmoreus strain and application thereof | |
| CN106635842B (en) | Armillaria mellea YN01(WT) and application thereof | |
| CN105802862B (en) | True pleurotus cornucopiae bacterial strain and its culture, cultural method and application | |
| CN109452088B (en) | Flammulina velutipes X18 and cultivation method thereof | |
| CN103202175A (en) | Novel strain of lyophyllum decastes | |
| CN107227263B (en) | White flammulina velutipes JK01 strain and application thereof | |
| CN107881118B (en) | Light yellow flammulina velutipes JK05 strain and application thereof | |
| CN106676016B (en) | The true pleurotus cornucopiae bacterial strain of the brown of high yield glucan and its cultural method and application | |
| CN103503780B (en) | New pure white hypsizigus marmoreus strain | |
| CN101647370A (en) | Standardized high-yield culture technique based on biological characteristics of asafetida mushroom | |
| CN106434382B (en) | Pure white true pleurotus cornucopiae bacterial strain and its molecular labeling, specific primer to and application | |
| CN103004464B (en) | Novel strain of hypsizigus marmoreus | |
| CN104206169A (en) | Method for preparing nutrient cereal by cordyceps militaris culture medium | |
| CN103503779B (en) | New pure white hypsizigus marmoreus strain | |
| CN104620855B (en) | Long handle oyster mushroom strain and culture medium, cultural method and application | |
| CN103289997B (en) | The molecule marker of pure white Hypsizygus marmoreus Finc-W-62 bacterial classification and preparation method thereof and application | |
| CN106434381B (en) | Pure white true pleurotus cornucopiae bacterial strain and its molecular labeling, specific primer to and application | |
| CN104710206A (en) | Preparation method for volvariella volvacea liquid strain | |
| CN103340156A (en) | Novel strain of lyophyllum decastes | |
| CN103155786A (en) | Screening method for brown mushroom stock-culture culture mediums | |
| CN103299912A (en) | New lyophyllum decaste strain | |
| CN103503778B (en) | New pure white hypsizigus marmoreus strain | |
| CN103289999B (en) | Molecular marker of pure white Hypsizygus marmoreus Finc-W-62, its acquisition method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |