CN103487415A - Monochromatic light beam excitation apparatus - Google Patents
Monochromatic light beam excitation apparatus Download PDFInfo
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- CN103487415A CN103487415A CN201210197180.8A CN201210197180A CN103487415A CN 103487415 A CN103487415 A CN 103487415A CN 201210197180 A CN201210197180 A CN 201210197180A CN 103487415 A CN103487415 A CN 103487415A
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- slit arrangement
- xenon lamp
- base plate
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Abstract
The invention discloses a monochromatic light beam excitation apparatus. The apparatus comprises a baseboard, a xenon light source device, a light splitting device and a light beam outputting device, the xenon light source device is arranged at one side of the baseboard, the light splitting device and the light beam outputting device are arranged at the other side of the baseboard, and light source light of a xenon lamp is processed to form a horizontal monochromatic light beam for emission. The apparatus is a horizontal monochromatic light beam excitation apparatus realizing integrated wide spectrum and adjustable wavelength and broadband.
Description
Technical field
The present invention relates to the optical detecting instrument technical field, particularly a kind of homogeneous beam excitation apparatus.
Background technology
The basic structure of fluorospectrophotometer is comprised of excitation source, excitation monochromator, emission monochromator, sample cell and detection system.Fluorospectrophotometer, by the light beam sent from light source, produces monochromatic light through excitation monochromator, and monochromatic light is radiated on sample, and the fluorescence sent by sample enters the emission monochromator, finally is incident upon on the detecting element photomultiplier.By the electric signal of photomultiplier output, through amplifier and register system, measure the fluorescence intensity of sample.
At present the each part mentioned above of fluorospectrophotometer mostly adopts the relatively independent structure of unit, and the easy like this structural stability of instrument that causes is poor, measures unstablely, and the general assembly debugging difficulty is high, and assembling speed is slow, the problems such as time cost height.In addition, if the monochromatic light that the excitation monochromator of fluorospectrophotometer produces is vertically to be radiated on sample, during measurement, required sample size is many and efficiency phosphor collection is low, makes the detection sensitivity of complete machine low.
In prior art, fluorospectrophotometer is by adopting laser, LED light lamp or narrow-cut filter to obtain the monochromatic excitation light source, cause like this excitation wavelength can not be continuously adjustable, excite the monochromatic light bandwidth to convert, the problem such as amount of samples is larger, limited the fluorescence analysis range of application.
Summary of the invention
The present invention overcomes the above defect of prior art, has proposed a kind of homogeneous beam excitation apparatus, comprising:
Base plate, which is provided with a hole;
Light source device for xenon lamp, it is fixedly installed on described base plate one side, for generation of the light source light of xenon lamp; The light source light of described xenon lamp sees through the opposite side that described hole shines described base plate;
Light-dividing device, the opposite side that it is arranged on described base plate, receive the light source light of described xenon lamp, and the light source light of described xenon lamp be treated to horizontal homogeneous beam;
The light beam output unit, it is arranged on described base plate and with described light-dividing device and is positioned at the same side, with described light-dividing device, is communicated with, for exporting described horizontal homogeneous beam.
Wherein, described light source device for xenon lamp comprises: the xenon lamp device; With the first condenser, its correspondence is arranged at the light source center position of described xenon lamp device.
Wherein, described light-dividing device comprises:
Catoptron;
The first slit arrangement, it is arranged on along on the light reflection direction of described catoptron;
Concave grating, it is arranged on along on the light reflection direction of described catoptron, after being positioned at described the first slit arrangement;
The second slit arrangement, it is arranged on along on the light reflection direction of described concave grating;
Drive unit, connect respectively the first slit arrangement, concave grating, the second slit arrangement;
Wherein, described the first slit arrangement, described concave grating, described the second slit arrangement, described drive unit are fixedly installed on described base plate; Described drive unit is fixedly installed on respectively on above-mentioned the first slit arrangement, the second slit arrangement.
Wherein, described drive unit comprises the first stepper motor, the second stepper motor, the 3rd stepper motor.
Wherein, described light-dividing device further comprises case, is provided with optical gate on it.
Wherein, described light beam output unit comprises second condenser lens; Described second condenser lens will be for converging to sample cell from the light beam of described the second slit arrangement outgoing.
Wherein, described light beam output unit further is fixedly connected on described base plate by cantilever.
Wherein, be provided with the deck structure on described cantilever, for fixing described light beam output unit.
Wherein, further comprise the supervision light path device, it comprises:
Piezoid, it is the dead astern that miter angle is arranged on described optical gate;
The 3rd condenser, it is arranged on along on the light refraction direction of described piezoid;
Photoelectric cell, it is arranged on along on the light refraction direction of described piezoid, after being positioned at described the 3rd condenser.
Thereby homogeneous beam excitation apparatus of the present invention is, by measuring fluorescence, test substance is carried out to qualitative and quantitative analysis, be a kind of be applicable to fluorospectrophotometer excite the monochromatic source device.The beneficial effect of homogeneous beam excitation apparatus of the present invention comprises: each building block of the whole device of the present invention is integrated being integrated on a base plate all, fully realizes the high density assembling of each building block, improved assembling speed, reduces man-hour and cost.Integrated apparatus of the present invention guarantees that its structural stability is high, and Measurement sensibility is good, and can complete in advance Installation and Debugging as a global facility, has improved the production efficiency of processing and fabricating.
In prior art, each parts arranges in one plane, and the present invention is mounted on respectively the both sides of erectting base plate by each building block with innovating, be about to the side that light source device for xenon lamp is arranged on base plate, light-dividing device and light beam output unit be arranged on to the opposite side of base plate simultaneously.The present invention adopts the light path design of three-dimensional uniquely, and output beam is regulated to control in the horizontal direction, thereby has significantly reduced the consumption of sample, has reduced expensive amount of samples, saves use cost.
The output beam of the horizontal direction that homogeneous beam excitation apparatus of the present invention obtains makes the collection efficiency of fluorescence high, adds the supervision light path device and realizes effective real-Time Compensation light source fluctuation and drift, has improved sensitivity of the present invention and signal to noise ratio (S/N ratio).Adjustable xenon lamp device provides the wide spectral characteristic of xenon lamp as light source, met the wide spectrum demand while using that detects.Utilize drive unit to regulate slit arrangement and concave grating, thereby wavelength and the bandwidth of regulation and control homogeneous beam have realized regulating continuously the wavelength of homogeneous beam and the purpose of bandwidth.The present invention has realized the horizontal homogeneous beam excitation apparatus that integrated wide spectrum and wavelength and bandwidth are adjustable.
The accompanying drawing explanation
Fig. 1 is the schematic cross-section of homogeneous beam excitation apparatus of the present invention.
Fig. 2 is the schematic diagram of the xenon lamp device in homogeneous beam excitation apparatus of the present invention.
Fig. 3 is the schematic diagram of the slit arrangement in homogeneous beam excitation apparatus of the present invention.
Fig. 4 is the schematic diagram of the cantilever in homogeneous beam excitation apparatus of the present invention.
Fig. 5 be light beam output unit in homogeneous beam excitation apparatus of the present invention and monitor light path device schematic diagram.
Fig. 6 is the light path schematic diagram of the light-dividing device in homogeneous beam excitation apparatus of the present invention.
Fig. 7 A is the schematic diagram that in prior art, sample cell receives normal beam.
Fig. 7 B is the schematic diagram that in the present invention, sample cell receives horizontal light beam.
Fig. 8 is the schematic diagram that the sector gear in homogeneous beam excitation apparatus of the present invention is meshed with stepping motor gear.
Fig. 9 is the light path schematic diagram of homogeneous beam excitation apparatus of the present invention.
Embodiment
Below in conjunction with drawings and Examples, specific embodiments of the present invention are further described in detail, but should not limit the scope of the invention with this.
As shown in Fig. 1-Fig. 9,1-xenon lamp device; 2-the first condenser; The 3-base plate; The 4-case; The 5-concave grating; 6-the first slit arrangement; The 7-catoptron; 8-the second slit arrangement; The 9-photoelectric cell; 10-the 3rd condenser, the 11-deck; The 12-second condenser lens; The 13-piezoid; The 14-cantilever; The 15-optical gate; The 16-microscope base; 19-the first stepper motor; The 20-gear; The 21-sector gear; 22-the second stepper motor, 23-the second stepper motor, 31-hole.
As shown in Figure 1, homogeneous beam excitation apparatus of the present invention can be used for conduct in fluorospectrophotometer and excites the monochromatic source device, comprising: base plate 3, light source device for xenon lamp, light-dividing device, light beam output unit.Base plate 3 is provided with a hole 31, and light can pass through this hole 31.Base plate 3 is to erect to be placed, and light source device for xenon lamp is arranged on a side of base plate 3, and light-dividing device, light beam output unit are arranged on the opposite side of base plate 3, realize the light path three-dimensional, make normal beam be transformed into horizontal light beam.
Light source device for xenon lamp comprises xenon lamp device 1 and the first condenser 2.Xenon lamp device 1 is to erect to be placed, and is fixedly installed on a side of base plate 3, and the light beam that xenon lamp produces can provide the wide spectral range light source.The first condenser 2 is fixed on base plate 3, with the corresponding setting in the light source center position of xenon lamp device 1.Light source focuses on through the first condenser 2, sees through the opposite side that hole 31 shines base plate 3.
Adjustable xenon lamp device 1 as shown in Figure 2, it forms, and patent of invention (patent No. CN 202189007 U) is described as has been publicly.Xenon lamp must vertically be arranged on base plate 3 one sides.Xenon lamp device 1 is fixed by bolts on base plate 3, and the first condenser 2 is arranged corresponding to the central axis of xenon lamp light emitting source.Xenon lamp light emitting source, hole 31, the first condenser 2 are coaxial setting.
Light-dividing device is arranged on the opposite side of base plate 3, receives the light source light of the xenon lamp of injecting by hole 31, and this xenon source light is treated to the homogeneous beam that meets request for utilization.Light-dividing device comprises the catoptron 7 that is arranged on base plate 3 opposite sides.The minute surface of reflective mirror 7 is 45 ° of mating holes 31, preferably, by the slit of beam reflection to the first slit arrangement 6 of the first condenser 2, makes focus just in time drop on the center of slit.
Light-dividing device also comprises: along the first slit arrangement 6 and the concave grating 5 that set gradually on the light reflection direction of catoptron 7, and be arranged on along the second slit arrangement 8 on the light reflection direction of concave grating 5, as shown in Figure 6.The first slit arrangement 6, concave grating 5, the second slit arrangement 8 can be bolted together on base plate 3.
Entrance slit and exit slit all play the light intensity of controlling monochromator and the effect that limits the outgoing beam spectral width.Automatically select the slit width of two slit arrangements, the light beam sent when xenon source converges to the first slit arrangement 6, only has the light beam of selected slit size to incide concave grating 5 through slit.By concave grating 5, this light beam is carried out to light-splitting processing, the continuous spectrum of xenon source is resolved to the single wavelength homogeneous beam in the 200nm-900nm scope, then reflex on the slit of the second slit arrangement 8, form horizontal homogeneous beam and penetrate.Comprised certain range of wavelengths in the light beam of outgoing from the selected slit of the second slit arrangement 8, the wavelength coverage in this is interval is called spectral width, and in the spectral width scope, the width of a half intensity is bandwidth.Therefore adjustable slit is also adjustable bandwidth.The bandwidth of instrument equals just to separate two wavelength distance of closing on spectral line, so the bandwidth of instrument meaned the resolution of instrument equally, has also meaned the degree of outgoing beam monochromatization.Minimum bandwidth of the present invention is 1nm, leads over fluorospectrophotometer common on market.In using the process of apparatus of the present invention, change the slit sizes of the first slit arrangement 6 and the second slit arrangement 8 by regulating drive unit, and the direction of concave grating 5, thereby adjust bandwidth and the wavelength of horizontal outgoing beam.
As shown in Figure 3, the first slit arrangement 6 and the second slit arrangement 8 are the slit arrangement that can automatically exchange different in width, and its structure is as published patent of invention (patent No. CN 201885800 U).Drive slit sheet by stepper motor, move horizontally the slit of selecting different in width, automatically adjustable to realize multiple slit bandwidth, can obtain the monochromatic light that various bandwidth is selected.
Light-dividing device also comprises the drive unit be fixed on base plate 3, as shown in Fig. 8,3, comprises the first stepper motor 19, the second stepper motor 22, the 3rd stepper motor 23, with power supply, is connected respectively.As shown in Figure 8, the first stepper motor 19 is connected with concave grating 5 with sector gear 21 by drive disk assembly gear 20.Concave grating 5 can be regulated in the 0-900nm wavelength coverage.Further, concave grating 5 is fixedly mounted on sector gear 21, and sector gear 21 can pivot.Gear 20 is installed on the shaft of the first stepper motor 19, and gear 20 meshes fully with sector gear 21, makes the first stepper motor 19 can drive concave grating 5 to be rotated.The first stepper motor 19 can be arranged on the homonymy of concave grating 5, also can be arranged on opposite side.The second stepper motor 22 is connected on the first slit arrangement 6, drives the slit sheet on the first slit arrangement 6 to make traveling priority, to convert the slit of mounted different in width in advance, to obtain the light beam of different light intensity degree.The 3rd stepper motor 23 is arranged on the second slit arrangement 8, drives the slit sheet on the second slit arrangement 8, and to obtain the light beam of different bandwidth, slit width of the present invention has some gears, every kind of corresponding a kind of bandwidth of slit width.Stepper motor, by computer control, can accurately be located the every gear slit of conversion.
Further, light-dividing device comprises case 4.Case 4 is made by light-proof material, guarantees that its content forms a darkroom.Case 4 is bolted together on base plate 3, can also other modes fix.Be provided with optical gate 15 on case 4, optical gate 15 is arranged on over against the position of the second slit arrangement 8, by computer control, opens optical gate 15 and can allow the homogeneous beam formed in light-dividing device pass through, closing homogeneous beam can not pass through, to meet demands different in test process.
The light beam output unit comprises second condenser lens 12.Second condenser lens 12 directly is fixed on base plate 3, and the focus of one side just in time overlaps setting with the slit of the second slit arrangement 8.The homogeneous beam penetrated after the second slit arrangement 8 is resolved, penetrate by optical gate 15, incides in second condenser lens 12, then penetrate by second condenser lens 12.
Behind relative this device of second condenser lens 12 location, further with two nuts 18, be spun on the external thread of second condenser lens 12 tail ends the side to deck 11, as shown in Figure 5, positioning anchor as it at this setting position, can change easily the second condenser lens 12 of selecting different size, to adapt to the difference of actual demand.
Preferably, by regulating cantilever 14, deck 11 or lid, the inboard focus of second condenser lens 12 is adjusted on the slit of the second slit arrangement 8, thereby form the horizontal homogeneous beam hot spot that a size is suitable with the slit sizes of the second slit arrangement 8 on the focus of the outside of second condenser lens 12, using this final output facula as the whole device formation of the present invention.The horizontal homogeneous beam penetrated from second condenser lens 12, have wavelength, bandwidth is adjustable, structural stability is high, Measurement sensibility is good characteristics.As shown in Figure 7 B, the schematic diagram of sample cell reception horizontal light beam in the present invention.The situation that receives normal beam with the prior art sample cell shown in Fig. 7 A is compared, and in sample cell of the present invention, the required sample size added obviously reduces, and the efficiency of phosphor collection improves greatly.
The present invention also comprises the supervision light path device, as shown in Figure 5, comprises piezoid 13, the 3rd condenser 10, photoelectric cell 9.Piezoid 13 is the dead astern that miter angle is arranged on optical gate 15, and the 3rd condenser 10 is successively set on along on the light refraction direction of piezoid 13 with photoelectric cell 9.
The homogeneous beam penetrated from the second slit arrangement 8, through optical gate 15, a part of homogeneous beam reflexes to the 3rd condenser 10 by piezoid 13, then through the 3rd condenser 10, this folded light beam is converged on photoelectric cell 9, forms and monitors optical path signal output.Variation by detecting photoelectric cell 9 is monitored the light intensity of homogeneous beam, and real-Time Compensation light source fluctuation and drift have improved sensitivity and the signal to noise ratio (S/N ratio) of apparatus of the present invention.
Simultaneously, the homogeneous beam penetrated by the second slit arrangement 8, another part homogeneous beam of process optical gate 15 is transmitted to second condenser lens 12, by penetrating with horizontal homogeneous beam after second condenser lens 12, is gathered on target sample, in order to detect sample.Now, the homogeneous beam converged on target sample is the level of state.As shown in Figure 7, the testing sample consumption is few, phosphor collection efficiency is high, and, by monitoring light path real-Time Compensation light source fluctuation and drift, sensitivity and the signal to noise ratio (S/N ratio) of whole device are greatly improved.
Further, be the fixed surveillance light path device, microscope base 16 is set on case 4, in order to fixedly piezoid 13 and the 3rd condenser 10.Microscope base 16 is arranged at the rear of homogeneous beam ejaculation place of the second slit arrangement 8.
Fig. 9 is the three-dimensional light path schematic diagram that apparatus of the present invention realize, its process is: the light beam of xenon lamp 1 is by the first condenser 2, converge to the slit center of the first slit arrangement 6 through catoptron 7 reflections through the hole 31 of erectting on base plate 3, light beam is projected to concave grating 5 through slit, through its reflection and focus to the slit center of the second slit arrangement 8, outgoing beam is by optical gate 15, part outgoing beam is transmitted through second condenser lens 12 from piezoid 13, and formed horizontal homogeneous beam converges to the sample cell center; Another part outgoing beam reflexes to the 3rd condenser 10 through piezoid 13, then converges to photoelectric cell 9.
The foregoing is only preferred embodiment of the present invention, not be used for limiting practical range of the present invention.Have in technical field and usually know the knowledgeable under any, without departing from the spirit and scope of the present invention, when being used for a variety of modifications and variations, so protection scope of the present invention should be looked claims institute confining spectrum and is as the criterion.
Claims (9)
1. a homogeneous beam excitation apparatus, is characterized in that, comprising:
Base plate (3), which is provided with a hole (31);
Light source device for xenon lamp, it is fixedly installed on described base plate (3) one sides, for generation of the light source light of xenon lamp; The light source light of described xenon lamp sees through the opposite side that described hole (31) shines described base plate (3);
Light-dividing device, the opposite side that it is arranged on described base plate (3), receive the light source light of described xenon lamp, and the light source light of described xenon lamp be treated to horizontal homogeneous beam;
The light beam output unit, it is arranged on described base plate (3) above and is positioned at the same side with described light-dividing device, with described light-dividing device, is communicated with, for exporting described horizontal homogeneous beam.
2. homogeneous beam excitation apparatus as claimed in claim 1, is characterized in that, described light source device for xenon lamp comprises:
Xenon lamp device (1);
The first condenser (2), its correspondence is arranged at the light source center position of described xenon lamp device (1).
3. homogeneous beam excitation apparatus as claimed in claim 1, is characterized in that, described light-dividing device comprises:
Catoptron (7);
The first slit arrangement (6), it is arranged on along on the light reflection direction of described catoptron (7);
Concave grating (5), it is arranged on along on the light reflection direction of described catoptron (7), is positioned at described the first slit arrangement (6) afterwards;
The second slit arrangement (8), it is arranged on along on the light reflection direction of described concave grating (5);
Drive unit (22,19,23), connect respectively the first slit arrangement (6), concave grating (5), the second slit arrangement (8);
Wherein, described the first slit arrangement (6), described concave grating (5), described the second slit arrangement (8), described drive unit (19) are fixedly installed on described base plate (3); Described drive unit (22,23) is fixedly installed on respectively on above-mentioned the first slit arrangement (6), the second slit arrangement (8).
4. homogeneous beam excitation apparatus as claimed in claim 3, is characterized in that, described drive unit (19,22,23) comprises the first stepper motor (19), the second stepper motor (22), the 3rd stepper motor (23).
5. homogeneous beam excitation apparatus as claimed in claim 1, is characterized in that, described light-dividing device further comprises case (4), is provided with optical gate (15) on it.
6. homogeneous beam excitation apparatus as claimed in claim 1, is characterized in that, described light beam output unit comprises second condenser lens (12); Described second condenser lens (12) will be for converging to sample cell from the light beam of described the second slit arrangement (8) outgoing.
7. homogeneous beam excitation apparatus as claimed in claim 1, is characterized in that, described light beam output unit further is fixedly connected on described base plate (3) by cantilever (14).
8. homogeneous beam excitation apparatus as claimed in claim 7, is characterized in that, is provided with deck structure (11) on described cantilever (14), for fixing described light beam output unit.
9. homogeneous beam excitation apparatus as claimed in claim 1, is characterized in that, further comprises the supervision light path device, and it comprises:
Piezoid (13), it is the dead astern that miter angle is arranged on described optical gate (15);
The 3rd condenser (10), it is arranged on along on the light refraction direction of described piezoid (13);
Photoelectric cell (9), it is arranged on along on the light refraction direction of described piezoid (13), is positioned at described the 3rd condenser (10) afterwards.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210197180.8A CN103487415A (en) | 2012-06-15 | 2012-06-15 | Monochromatic light beam excitation apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210197180.8A CN103487415A (en) | 2012-06-15 | 2012-06-15 | Monochromatic light beam excitation apparatus |
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| CN103487415A true CN103487415A (en) | 2014-01-01 |
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| CN201210197180.8A Pending CN103487415A (en) | 2012-06-15 | 2012-06-15 | Monochromatic light beam excitation apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107588363A (en) * | 2017-08-24 | 2018-01-16 | 中国电子科技集团公司第四十研究所 | The polarized monochromatic light source generation system and method for a kind of Programmable and Variable wavelength |
| CN109358000A (en) * | 2018-10-23 | 2019-02-19 | 福建信息职业技术学院 | A kind of light emitting device and a variety of monochromatic methods of output of dual hump type spectrum |
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| CN1065523A (en) * | 1991-03-29 | 1992-10-21 | 株式会社岛津制作所 | Spectrophotometer |
| CN2641611Y (en) * | 2003-09-12 | 2004-09-15 | 北京普析通用仪器有限责任公司 | Light source device for atomic absorption spectrophotometer |
| CN1912579A (en) * | 2006-08-30 | 2007-02-14 | 陈明 | COD photoelectric detection device |
| CN101275869A (en) * | 2008-05-06 | 2008-10-01 | 北京普析科学仪器有限责任公司 | Monochromator |
| CN201885800U (en) * | 2010-11-17 | 2011-06-29 | 上海棱光技术有限公司 | Slit device capable of automatically interchanging different widths |
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2012
- 2012-06-15 CN CN201210197180.8A patent/CN103487415A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1065523A (en) * | 1991-03-29 | 1992-10-21 | 株式会社岛津制作所 | Spectrophotometer |
| CN2641611Y (en) * | 2003-09-12 | 2004-09-15 | 北京普析通用仪器有限责任公司 | Light source device for atomic absorption spectrophotometer |
| CN1912579A (en) * | 2006-08-30 | 2007-02-14 | 陈明 | COD photoelectric detection device |
| CN101275869A (en) * | 2008-05-06 | 2008-10-01 | 北京普析科学仪器有限责任公司 | Monochromator |
| CN201885800U (en) * | 2010-11-17 | 2011-06-29 | 上海棱光技术有限公司 | Slit device capable of automatically interchanging different widths |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107588363A (en) * | 2017-08-24 | 2018-01-16 | 中国电子科技集团公司第四十研究所 | The polarized monochromatic light source generation system and method for a kind of Programmable and Variable wavelength |
| CN109358000A (en) * | 2018-10-23 | 2019-02-19 | 福建信息职业技术学院 | A kind of light emitting device and a variety of monochromatic methods of output of dual hump type spectrum |
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Application publication date: 20140101 |