CN103479987A - Preparation method and antioxidation and bacteriostatic actions of ethanol extract from ginger - Google Patents
Preparation method and antioxidation and bacteriostatic actions of ethanol extract from ginger Download PDFInfo
- Publication number
- CN103479987A CN103479987A CN201310459033.8A CN201310459033A CN103479987A CN 103479987 A CN103479987 A CN 103479987A CN 201310459033 A CN201310459033 A CN 201310459033A CN 103479987 A CN103479987 A CN 103479987A
- Authority
- CN
- China
- Prior art keywords
- rhizoma zingiberis
- zingiberis recens
- ginger
- ethanol extraction
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a preparation method and antioxidation and bacteriostatic actions of an ethanol extract from ginger. The preparation method is characterized in that the ginger and a ginger product are taken as raw materials and extracted by 60-99% of ethanol, ethanol is recycled, and the extract or dry powder is prepared. The ethanol extract from the ginger contains more gingerol. The experiment proves that the ethanol extract from the ginger has obvious antioxidation and peroxy radical clearing actions and also has obvious bacteriostatic action on staphylococcus aureus, colibacillus, pseudomonas aeruginosa and bacillus subtilis. The ethanol extract from the ginger is a natural antioxidative and antibacterial substance and has a certain application prospect in safe, effective and harmfulless natural food additives.
Description
Technical field
The present invention relates to a kind of preparation of Jiang Yi ethanol extract and in antioxidation and bacteriostasis.Belong to biological technical field.
Background technology
Rhizoma Zingiberis Recens (
zingiber officinalerosc.) be that Zingiberaceae, Rhizoma Zingiberis Recens belong to herbaceos perennial, have another name called ground suffering, hundred peppery clouds, be considered as the conventional Chinese medicine of dietotherapeutic from ancient times by the physician.The chemical composition of Rhizoma Zingiberis Recens mainly contains ethereal oil and piquancy component two large classes [3-4], mainly contains the phenols components [5-7] such as gingerol (gingerol), shogaol (shogaol) and zingiberone (zingerone) in piquancy component.Medical research shows, Rhizoma Zingiberis Recens has the medicinal health functions [8-10] such as obvious analgesia, analgesic, antiinflammatory, antitumor, antifungal, antioxidation, blood fat reducing, blood sugar lowering and anti-gastric-ulcer.Its aboundresources, therefrom extract natural anti-oxidation and antipathogenic composition efficient, economic security, than the traditional chemical inhibitor, compares, the antioxidation antibacterial substance wide material sources that this class is natural, process cheap, safety non-toxic, this is significant to clinical drug and food industry.This research, by contrast fresh ginger, Rhizoma Zingiberis, the antioxidant activity of three kinds of Rhizoma Zingiberis Recens products of Rhizoma Zingiberis Recens sugar and the difference of fungistatic effect, lays the foundation in the application of food and medical domain for further studying and develop different Rhizoma Zingiberis Recens products.
Source investigation shows, in current medical research, whether a lot of chronic diseases and Free Radical Metabolism of Human Body be normally closely related, free radical is necessary by maintaining normal life, it is also the killer of biomacromolecule, nucleus biological tissue, removes unnecessary free radical in body and is conducive to health [1-2].Therefore, develop new natural food and the medicine that can remove safely and effectively free radical unnecessary in human body and become one of modern study focus.
Medical research shows, the inducement of numerous disease may be relevant with the oxidation product of lipid in organism or in food, except the mankind being threatened to maximum cardiovascular and cerebrovascular disease and tumor, the disease relevant with free radical also has hundreds of, as aging, sudden change and inflammation [3] etc.Therefore, efficient, the low toxicity of exploitation, inexpensive antioxidant activity medicine or food are important research directions.
Although the food preservative potassium sorbate be widely used at present is comparatively safe effectively, its consumption is difficult to strict control, poor to bacterial action, and consumption can affect flavour of food products [4] when excessive.Also do not have at present a kind of antiseptic can effectively suppress all microorganisms.Therefore, safety, natural additive for foodstuff effective, low toxicity are popular at present research topics.
Summary of the invention
The invention provides a kind of extraction preparation method of Rhizoma Zingiberis Recens ethanol extraction, be applicable to suitability for industrialized production.
A kind of Rhizoma Zingiberis Recens ethanol extraction provided by the invention, the alcohol extract of this Rhizoma Zingiberis Recens mainly contains gingerol.
A kind of Rhizoma Zingiberis Recens ethanol extraction provided by the invention, the effect of this Rhizoma Zingiberis Recens ethanol extraction to thering is antioxidation, removing DPPH free radical ABTS free radical.
A kind of Rhizoma Zingiberis Recens ethanol extraction provided by the invention, this extract has significant bacteriostasis to staphylococcus aureus, escherichia coli, bacillus pyocyaneus and bacillus subtilis.
The present invention further discloses the effect of the disease that the Rhizoma Zingiberis Recens ethanol extraction causes by peroxidating in prevention, treatment and auxiliary treatment, and the application of the alcohol extract of Rhizoma Zingiberis Recens in antioxidant activity medicine or food.
The present invention further discloses the Rhizoma Zingiberis Recens ethanol extraction in safety, effectively, the application in the natural additive for foodstuff of low toxicity.
rhizoma Zingiberis Recens provided by the inventionsecond
the preparation method of alcohol extract, concrete technical scheme is as follows:
Take Rhizoma Zingiberis as benchmark, take a certain amount of Rhizoma Zingiberis or other ginger productses (comprising fresh ginger, old ginger etc.), take 60-99% ethanol as solvent, under feed liquid mass ratio 1:12.5-1:20 condition, at 25-40 ℃, supersound process 30-50 min under the condition of 50HZ, 250W, sucking filtration, the residue solubilizer is supersound process 30-50 min again, and merging filtrate heating are concentrated into drying, and the dry product obtained is the alcohol extract of Rhizoma Zingiberis Recens.
rhizoma Zingiberis Recens of the present inventionsecond
the antioxidation of alcohol extract, is characterized in thattake the gingerol class as main effective ingredient.In the present invention, we have measured Rhizoma Zingiberis Recens ethanol extraction antioxidation activity in vitro antibacterial action.Result shows, the alcohol extract of Rhizoma Zingiberis Recens has stronger antioxidant activity in vitro, and has obvious bacteriostasis.The gingerol compounds that this activity of Rhizoma Zingiberis Recens ethanol extraction may be summed up in the inner is relevant.
good effect of the present invention is: take Rhizoma Zingiberis Recens as raw material, adopt conventional extracting method, easy being easy to get, the alcohol extract of Rhizoma Zingiberis Recens provided by the invention has strong antioxidant action, the research and development of antioxidant food, antimicrobial.The alcohol extract of Rhizoma Zingiberis Recens provided by the invention has antioxidation and can be applied in the antioxidation disease treatment, also can be used as natural antibiotic preservative and is applied in food processing.
To bacteriostasis and antioxidation, below provide gingerol assay and antioxidation and bacteriostatic experiment result in the Rhizoma Zingiberis Recens ethanol extraction for the alcohol extract that further illustrates Rhizoma Zingiberis Recens of the present invention.
gingerol content comparative measurements in experimental example 1 Jiang Yi ethanol extract
1 experiment material
The Rhizoma Zingiberis Recens ethanol extraction: the Rhizoma Zingiberis Recens of take processes respectively as raw material adopts method in embodiment 1,2,3.
2 extracting method
Reference substance solution list of references method [5], accurately take 10 mg vanillin, with the dehydrated alcohol dilution, is mixed with the vanillin standard solution that mass concentration is 0.2 mg/mL.Draw respectively this standard solution 0.1,0.2,0.3,0.4,0.5 mL in 10 mL volumetric flasks, the dehydrated alcohol standardize solution, concussion shakes up.Take dehydrated alcohol as blank, at 280 nm wavelength places, measure absorbance, drawing standard curve.Linear regression obtains the standard curve equation y=1.3937x+0.0002 of vanillin, coefficient R
2=0.9989. the method linear relationship in vanillin concentration is 2～12 μ g/mL scopes is good.
The mensuration of gingerol content in sample: 0.1 mL is in 10 mL volumetric flasks for accurate absorption sample liquid, the dehydrated alcohol standardize solution, and concussion shakes up.Dehydrated alcohol is blank, at 280 nm wavelength places, measures absorbance, obtains vanillin content by regression equation, then is multiplied by the content that conversion coefficient 2.003 is gingerol.
4 results
Gingerol content in different Rhizoma Zingiberis Recens product ethanol extractions
Get the ethanol extraction of fresh ginger, Rhizoma Zingiberis, Rhizoma Zingiberis Recens sugar, according to the method for " 2 ", prepare the detection that test liquid carries out gingerol content, testing result is that the gingerol content in the fresh ginger ethanol extraction is 1.97%, and the gingerol in Rhizoma Zingiberis Recens sugar ethanol extraction is 0.65%
Gingerol content in the alcohol extract of Rhizoma Zingiberis is up to 3.02 %.
experimental example 2 Rhizoma Zingiberis Recenssecond
the alcohol extract antioxidation activity in vitro is measured
The preparation of 1 test sample
Take Rhizoma Zingiberis as benchmark, composition proportion by fresh ginger water content and Rhizoma Zingiberis Recens sugar is calculated, accurately take fresh ginger (Ginger, G) 4.5 g, Rhizoma Zingiberis (Dried ginger, DG) 1 g, Rhizoma Zingiberis Recens sugar (Candied ginger, CG) 11.5 g(Rhizoma Zingiberis Recens: brown sugar: starch=1:8:3), according to embodiment 1, embodiment 2, embodiment 3 method operations, 60% ethanol is solvent, supersound process 30 min under solid-liquid ratio 1:12.5 condition, sucking filtration, the residue solubilizer is processed 30 min again, and merging filtrate is positioned over heating on water-bath and is concentrated into evaporate to dryness.The dry product obtained is mixed with to the test liquid that mass concentration is respectively 0.05,0.1,0.3,0.5,0.8,1,5,10 mg/mL.
Positive drug: accurately take a certain amount of BHT(reference substance) be mixed with the solution that mass concentration is respectively 0.05,0.1,0.3,0.5,0.8,1,5,10 mg/mL.
Antioxidant activity experimental technique list of references method [6-8].
2 DPPH remove free radical activity and measure
Accurately take the DPPH powder, be mixed with the storing solution of 2 * 10-4 mg/mL with dehydrated alcohol.In reaction system, add sample and the reference substance of 2 mL variable concentrations in 10 mL test tubes, then add 7.5 mL DPPH solution, concussion shakes up.Under room temperature, lucifuge is reacted 1 h, in 517 nm places, measures its absorbance, is designated as A1, and the blank group replaces sample with 2 mL60% ethanol, records absorbance, is designated as A2.The experiment triplicate, the mapping of averaging.
DPPH free radical scavenging activity (I%)=(A2-A1)/A2 * 100%
3 ABTS remove free radical activity and measure
Deionized water is mixed with the ABTS solution of 7 mmol/L and the potassium persulfate solution of 2.45 mmol/L, gets potassium persulfate solution 3 ml and joins in 9 mLABTS solution, and at room temperature lucifuge reaction 12-16 h, form ABTS free radical storing solution.In 734 nm places, the ethanol that is 60% by volume fraction is diluted to absorbance by ABTS free radical storing solution and is 0.7 ± 0.05, standby.In reaction system, add sample and the reference substance of 0.8 mL variable concentrations in 10 mL test tubes, then add 7 mLABTS+ solution, concussion mixes.Under room temperature, lucifuge is reacted 30 min, in 734 nm places, measures its absorbance, is designated as Ai, and the blank group replaces sample with 0.8 mL60% ethanol, records absorbance, is designated as As.The experiment triplicate, the mapping of averaging.
ABTS free radical scavenging activity (I%)=(As-Ai)/As * 100
4 reducing powers are measured
In reaction system, add sample and the reference substance of 0.2 mL variable concentrations in 10 mL test tubes, then add phosphate buffer and 2.5 mL 1% potassium ferricyanides of 2.5 mL 0.2 M pH6.6, concuss, mix rear 50 ℃ of lower water-bath 20 min.After taking-up, after the refrigeration, in system, add rapidly the trichloroacetic acid of 2.5 mL 10%, after mixing under 4200 rmp centrifugal 10 min.Get supernatant 2 mL in the test tube of 10 mL, add the ferric chloride of 2.5 mL distilled water and 0.5 mL 0.1%, after 10 min, in 700 nm places, measure its absorbance.The absorbance of system is higher shows that reducing power is stronger.The experiment triplicate, the mapping of averaging.
5 results
(1) DPPH removes free radical activity
As shown in Figure 1, Rhizoma Zingiberis Recens product ethanol extraction has certain removing effect to the DPPH free radical, and, along with the increase of concentration, removes effect and strengthen gradually, and when reaching certain concentration, the removing effect of Rhizoma Zingiberis and fresh ginger reaches unanimity.Each sample scavenging ability of DPPH free radical is BHT ﹥ Rhizoma Zingiberis ﹥ fresh ginger ﹥ Rhizoma Zingiberis Recens sugar.
(2) ABTS removes free radical activity
As shown in Figure 2, Rhizoma Zingiberis, fresh ginger ethanol extraction have stronger removing effect to the ABTS free radical, and, along with the increase of concentration, remove ABTS free radical ability stronger, and when reaching certain concentration, Rhizoma Zingiberis is tending towards parallel with the removing effect of fresh ginger.When concentration is 0.8 mg/mL, the clearance rate of Rhizoma Zingiberis and fresh ginger is all more than 90%, a little less than positive drug BHT.It is BHT ﹥ Rhizoma Zingiberis ﹥ fresh ginger ﹥ Rhizoma Zingiberis Recens sugar that each sample is removed ABTS free radical ability.
(3) reducing power is measured
In the mensuration of total reducing power, the absorbance of the larger sample of reducing power is higher, and its antioxidant activity is just stronger.As shown in Figure 3, when concentration range, during at 0～0.1 mg/mL, the antioxidant activity between sample and BHT differs less, but, along with the increase of concentration, the antioxidant activity gap between the two is obvious gradually; When concentration range, during at 0～1.0 mg/mL, the antioxidant activity between sample differs less, but, along with the increase of concentration, the antioxidant activity gap between each sample becomes large gradually.Each sample reducing power ability is BHT ﹥ Rhizoma Zingiberis ﹥ fresh ginger ﹥ Rhizoma Zingiberis Recens sugar.
the Antibacterial Activity of the ethanol extract of experimental example 4 Rhizoma Zingiberis Recens
1 bacteriostasis assay method
(1) culture medium preparation
Beef extract-peptone solid (liquid) culture medium: Carnis Bovis seu Bubali cream 12 g, peptone 40 g, sodium chloride 20 g, agar 80 g, water 4000 mL.The culture medium configured is through 121 ℃, high temperature sterilize 20 min.
(2) preparation of test sample
The dry product that embodiment 1, embodiment 2, embodiment 3 are obtained is mixed with respectively the test liquid that mass concentration is respectively 10,30,50 mg/mL.
Positive drug: accurately take the solution that a certain amount of streptomycin (Streptomycin SM reference substance) is mixed with 3 mg/mL.
(3) preparation of bacteria suspension
Under the superclean bench aseptic condition, get a ring bacterium liquid with inoculating loop in the test tube that indicator bacteria (staphylococcus aureus Staphylococcus aureus, escherichia coli Escherichia coli, bacillus pyocyaneus Pseudomonas aeruginosa, bacillus subtilis Bacillus subtilis) is housed, join in the beef extract-peptone fluid medium, in constant incubator, 37 ℃ of static cultivation 24 h.Cultured bacterium liquid is diluted to sterilized water that to contain the bacterium amount be 105-106 cfu/mL(blood cell counting plate counting) suspension.
(4) bacteriostatic experiment
This experiment adopts filter paper method of diffusion [9].Filter paper is broken into to the filter paper that diameter is 6 mm with card punch, and 165 ℃ of dry heat sterilizations are standby.In superclean bench, by not solidified beef extract-peptone solid medium after sterilizing, pour into uniformly in culture dish, after cooling, get 0.1 mL bacteria suspension with liquid-transfering gun, bacteria suspension is squeezed in culture dish, placed 5 min left and right triangle spreaders, coating evenly.Again the aseptic filter paper sheet that is soaked with respectively Rhizoma Zingiberis Recens product ethanol extraction solution, positive drug solution (streptomycin solution), 60% ethanol contrast solution and blank solution (sterilized water) is dried, with aseptic nipper, be placed in on the examination flat board, each flat board is divided into 4 zones, and each test sample arranges 3 repetitions.The sealed membrane sealing, be placed in constant incubator, cultivates 24 h, Measurement accuracy antibacterial circle diameter (mm) for 37 ℃.
5. result
The bacteriostatic activity of test sample and positive control is in Table 2.
As shown in table 1,3 kinds of test samples are the strongest to the inhibitory action of staphylococcus aureus, are secondly bacillus pyocyaneus, bacillus subtilis, and wherein only Rhizoma Zingiberis has fainter inhibitory action to escherichia coli; Each population of samples fungistatic effect by by force to a little less than be followed successively by: streptomycin ﹥ Rhizoma Zingiberis ethanol extraction ﹥ fresh ginger ethanol extraction ﹥ Rhizoma Zingiberis Recens sugar ethanol extraction.
The inhibitory action of each sample of table 1 to antibacterial
Annotate: "+" has significant inhibitory action; "～" is without significant inhibitory action; "-" unrestraint effect; Numerical value be inhibition zone size (diameter, mm).
The ethanol extraction of Rhizoma Zingiberis Recens has significant antioxidation activity in vitro and bacteriostatic activity.The wherein bacteriostasis of Rhizoma Zingiberis Recens product and antioxidation and wherein the content positive correlation of gingerol, the antioxidation of the ethanol extraction of Rhizoma Zingiberis Recens and bacteriostasis may be summed up gingerol compounds in the inner.
The accompanying drawing explanation
Fig. 1. the DPPH of Rhizoma Zingiberis Recens ethanol extraction removes free radical activity measurement result figure
Fig. 2. the ABTS of Rhizoma Zingiberis Recens ethanol extraction removes free radical activity measurement result figure
Fig. 3. the reducing power measurement result figure of Rhizoma Zingiberis Recens ethanol extraction
The specific embodiment
For the ease of understanding the present invention, especially exemplified by following examples.Its effect is understood to be to explaination of the present invention but not to any type of restriction of the present invention.
embodiment 1 Rhizoma Zingiberis Recenssecond
the preparation of alcohol extract
Take the Rhizoma Zingiberis of 100g, add the ethanol of 1.25L 60%, at 40 ℃, supersound process 30 min under the condition of 50HZ, 250W, sucking filtration, the residue solubilizer is supersound process 30 min again, merging filtrate heating are concentrated into drying, and the dry product obtained is the Rhizoma Zingiberis Recens ethanol extraction.
embodiment 2 Rhizoma Zingiberis Recenssecond
the preparation of alcohol extract
Take the 100g fresh ginger, take 60% ethanol as solvent, under feed liquid mass ratio 1:12.5 condition, at 40 ℃, supersound process 30 min under the condition of 50HZ, 250W, sucking filtration, the residue solubilizer is supersound process 30 min again, and merging filtrate heating are concentrated into drying, and the dry product obtained is the Rhizoma Zingiberis Recens ethanol extraction.
embodiment 3 Rhizoma Zingiberis Recenssecond
the preparation of alcohol extract
Take 100g Rhizoma Zingiberis Recens sugar, take 60% ethanol as solvent, under feed liquid mass ratio 1:12.5 condition, at 40 ℃, supersound process 30min under the condition of 50HZ, 250W, sucking filtration, the residue solubilizer is supersound process 30min again, and merging filtrate heating are concentrated into drying, and the dry product obtained is the Rhizoma Zingiberis Recens ethanol extraction.
embodiment 4 Rhizoma Zingiberis Recenssecond
the preparation of alcohol extract
Take the 100g Rhizoma Zingiberis, take 80% ethanol as solvent, under feed liquid mass ratio 1:15 condition, at 40 ℃, supersound process 30 min under the condition of 50HZ, 250W, sucking filtration, the residue solubilizer is supersound process 30min again, and merging filtrate heating are concentrated into drying, and the dry product obtained is the Rhizoma Zingiberis Recens ethanol extraction.
embodiment 5 Rhizoma Zingiberis Recenssecond
the application in natural antiseptic agent of alcohol extract
In embodiment 1, the extract of prepared Rhizoma Zingiberis Recens is as natural antibacterial, and sterilizing, pack and get final product.Coordinate other antibiotic antiseptics, the alcohol extract of Rhizoma Zingiberis Recens plays the antibiotic effect of antioxidation.
list of references
[1] Li Yunong. Malus research of fruit germplasm resource [M]. the .2001. of Chinese agriculture publishing house 67.
[2] physiological reaction and the evaluation of resistance [D] of tetra-kinds of northern deciduous trees nursery stocks of Ma Shuhua to automobile exhaust pollution. Heilungkiang. Northeast Forestry University. 2004.
[3] Zeng T, Xie YX, Ma L. Toxicological evaluation of potassium sorbate [J].
HIMedJ, 2012, 23(19): 19-21.
[4] Wang WH, Li J, Gao HM,
et al. Chemical ingredients change of ginger processing with HPLC specific chromatogram [J].
Chin J Pharm Anal, 2009, 9(8): 1248-1249.
[5] Tang SR, Song H, Liu QD,
et al. Ultrasonic-assisted Extraction and Antioxidant Activity of Gingerol [J].
Dep Food, 2009, 30(20): 138-139.
[6] Stoilova I, Krastanov A, Stoyanova A,
et al. Antioxidant activity of a ginger extract (Zingiber officinale) [J].
Food Chem, 2007, 102(3): 764-770.
[7] He JS, Huang B, Ban XQ,
et al. In vitro and in vivo antioxidant activity of the ethanolic extract from Meconopsis quintuplinervia [J].
J Ethnopharmacol, 2012, 141(2): 104-110.
[8] Huang B, Ke HB, He JS,
et al. Extracts of
Halenia elliptica exhibit antioxidant properties in vitro and in vivo [J].
Food ChemToxicol, 2011, 49(6): 185-190.
[9] Liu XY, Bai WD. Research progress on the bacteriostasis and anti-oxidation of cinnamon essential oil [J].
FoodMach, 2010, 26(5): 169-171.
Claims (7)
1. the extraction preparation method of a Rhizoma Zingiberis Recens ethanol extraction, be applicable to suitability for industrialized production, it is characterized in that for Rhizoma Zingiberis Recens and the preparation of Rhizoma Zingiberis Recens product, concrete grammar: take Rhizoma Zingiberis as benchmark, take a certain amount of Rhizoma Zingiberis or other ginger productses (comprising fresh ginger, old ginger etc.), take 60-99% ethanol as solvent, under feed liquid mass ratio 1:12.5-1:20 condition, at 25-40 ℃, supersound process 30-50 min under the condition of 50HZ, 250W, sucking filtration, the residue solubilizer is supersound process 30-50 min again, merging filtrate heating are concentrated into drying, and the dry product obtained is the alcohol extract of Rhizoma Zingiberis Recens.
2. according to claim 1, gained Rhizoma Zingiberis Recens ethanol extraction is rich in gingerol.
3. according to claim 1, gained Rhizoma Zingiberis Recens ethanol extraction have remove the DPPH free radical, remove the ABTS free radical, antioxidation.
4. according to claim 1, gained Rhizoma Zingiberis Recens ethanol extraction, this extract has significant bacteriostasis to staphylococcus aureus, escherichia coli, bacillus pyocyaneus and bacillus subtilis.
5. according to claim 1, the solvent that the Rhizoma Zingiberis Recens ethanol extraction adds in leaching process is the second alcohol and water, and ethanol content is 60%-99%.
6. the effect of the disease caused by peroxidating in prevention, treatment and auxiliary treatment according to claim 1 gained Rhizoma Zingiberis Recens ethanol extraction, and the application of Rhizoma Zingiberis Recens ethanol extraction in antioxidant activity medicine or food.
7. according to the application of claim 1 gained Rhizoma Zingiberis Recens ethanol extraction in safe and effective wholefood antibacterial.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310459033.8A CN103479987A (en) | 2013-10-07 | 2013-10-07 | Preparation method and antioxidation and bacteriostatic actions of ethanol extract from ginger |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310459033.8A CN103479987A (en) | 2013-10-07 | 2013-10-07 | Preparation method and antioxidation and bacteriostatic actions of ethanol extract from ginger |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103479987A true CN103479987A (en) | 2014-01-01 |
Family
ID=49820800
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310459033.8A Pending CN103479987A (en) | 2013-10-07 | 2013-10-07 | Preparation method and antioxidation and bacteriostatic actions of ethanol extract from ginger |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103479987A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103815014A (en) * | 2014-03-13 | 2014-05-28 | 海南省粮油科学研究所 | Preparation method of natural rice preservatives |
| CN109970536A (en) * | 2019-04-22 | 2019-07-05 | 温州市人民医院 | A kind of extracting method of rhizoma zingiberis effective component |
| CN110483844A (en) * | 2019-09-04 | 2019-11-22 | 湖南宇山玉月农业科技有限公司 | A kind of oxidation-resistant film |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101264311A (en) * | 2008-04-21 | 2008-09-17 | 青岛大学 | Natural pharmaceutical composition, its preparation and its attenuation synergistic use in cancer radiotherapy and chemotherapy |
| CN102091314A (en) * | 2011-03-02 | 2011-06-15 | 青岛大学 | Composition of dried ginger and evodia rutaecarpa, preparation method thereof and application thereof in preparing attenuating synergistic medicament in radiotherapy and chemotherapy of cancer |
-
2013
- 2013-10-07 CN CN201310459033.8A patent/CN103479987A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101264311A (en) * | 2008-04-21 | 2008-09-17 | 青岛大学 | Natural pharmaceutical composition, its preparation and its attenuation synergistic use in cancer radiotherapy and chemotherapy |
| CN102091314A (en) * | 2011-03-02 | 2011-06-15 | 青岛大学 | Composition of dried ginger and evodia rutaecarpa, preparation method thereof and application thereof in preparing attenuating synergistic medicament in radiotherapy and chemotherapy of cancer |
Non-Patent Citations (2)
| Title |
|---|
| 张红霞等: "生姜醇提物药理学研究进展", 《中医研究》, vol. 21, no. 3, 31 March 2008 (2008-03-31), pages 60 - 62 * |
| 邓开野等: "生姜的抗菌及抗氧化作用的研究进展", 《中国调味品》, vol. 37, no. 2, 31 December 2012 (2012-12-31), pages 28 - 31 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103815014A (en) * | 2014-03-13 | 2014-05-28 | 海南省粮油科学研究所 | Preparation method of natural rice preservatives |
| CN109970536A (en) * | 2019-04-22 | 2019-07-05 | 温州市人民医院 | A kind of extracting method of rhizoma zingiberis effective component |
| CN110483844A (en) * | 2019-09-04 | 2019-11-22 | 湖南宇山玉月农业科技有限公司 | A kind of oxidation-resistant film |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fernández-Agulló et al. | Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts | |
| Gomaa | In vitro antioxidant, antimicrobial, and antitumor activities of bitter almond and sweet apricot (Prunus armeniaca L.) kernels | |
| Daneshzadeh et al. | An investigation on phytochemical, antioxidant and antibacterial properties of extract from Eryngium billardieri F. Delaroche | |
| Soare et al. | Antioxidant activity, polyphenols content and antimicrobial activity of several native pteridophytes of Romania | |
| Amin et al. | In-vitro antioxidant and antibacterial activities of pumpkin, quince, muskmelon and bottle gourd seeds | |
| Monon et al. | Phytochemical composition, antioxidant and antibacterial activities of root of Uvaria chamae P. Beauv.(Annonaceae) used in treatment of dysentery in North of Côte d’Ivoire | |
| Česonienė et al. | Antimicrobial activity of Viburnum opulus fruit juices and extracts | |
| Jain et al. | Comparative evaluation of Ziziphus mauritiana leaf extracts for phenolic content, antioxidant and antibacterial activities | |
| CN109700679A (en) | Application of the plant-derived compound preservative of food-grade in cosmetics | |
| Karamian et al. | Essential oil compositions and in vitro antioxidant and antibacterial activities of the methanol extracts of two Salvia species (Lamiaceae) from Iran | |
| CN107961399A (en) | A kind of water-based body lotion containing complex antimicrobials | |
| CN103479987A (en) | Preparation method and antioxidation and bacteriostatic actions of ethanol extract from ginger | |
| CN101223891A (en) | Aniseed essential oil for inhibiting plant pathogenic fungi and preparing method thereof | |
| Sökmen et al. | Antioxidant and antibacterial properties of a lichen species Diploschistes scruposus (Schreb.) Norman | |
| Rachana et al. | Antioxidant and bactericidal activity of wild turmeric extracts | |
| Omodamiro et al. | Antioxidant and antibacterial activities of Ocimum gratissimum | |
| Elgammal et al. | Mechanism of action and bioactivities of Cinnamomum zeylanicum essential oil against some pathogenic microbes | |
| Drăgan et al. | Evaluating in vitro antibacterial and antioxidant properties of Origanum vulgare volatile oil | |
| Chama et al. | Effect of Thymus vulgaris oil on the growth of Helicobacter pylori. | |
| WO2023036167A1 (en) | Torreya grandis compound oil, manufacturing method therefor and application thereof | |
| Ayari et al. | Evaluation of antioxidant and antimicrobial activities of Tunisian Ajuga iva L. essential oils | |
| Thebo et al. | Clinical study of the prunus dulcis (Almond) shell extract on tinea capitis infection | |
| CN105124716A (en) | Method of preparing food preservative through extraction waste liquid of cinnamon oil | |
| Tharun et al. | Evaluation of antioxidant potential and antimicrobial activity of successive extracts of Pimpinella tirupatiensis | |
| El-Demery et al. | Efficiency of propolis and turmeric powders as natural preservatives in minced beef |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140101 |