CN103479401B - A kind of intestinal biomarker sampler - Google Patents
A kind of intestinal biomarker sampler Download PDFInfo
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- CN103479401B CN103479401B CN201310456050.6A CN201310456050A CN103479401B CN 103479401 B CN103479401 B CN 103479401B CN 201310456050 A CN201310456050 A CN 201310456050A CN 103479401 B CN103479401 B CN 103479401B
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Abstract
The invention discloses a kind of intestinal biomarker sampler, comprising: a shell, in capsule-type; One inner shell, is positioned at shell, is close to the shape adaptation of shell with outer casing inner wall; Multiple microsphere, is filled in inner shell, and the outer surface of described microsphere is with the ligands specific film be combined with outside biomarker; Adsorb the outside biomarker with its pairing by the ligands specific film of microsphere surface, realize the sampling of intestinal biomarker.The present invention can sample to the biomarker in the Excreta in intestinal easy, automatically quickly and safely.
Description
Technical field
The present invention relates to can be oral mini-medical apparatus, particularly a kind of intestinal biomarker sampler.
Background technology
In medicinal practice, the test in laboratory of biomarker occupies very consequence in modern medicine.At present, existing increasing biomarker is found and is widely used in clinical practice, these biomarkers may come from tissue, organ, blood, bile, bone marrow, urine or feces etc., the variation of lung cancer tumor gene can be understood, direction of medication usage the prognosis of predicting tumors as the EGF-R ELISA (EGFR) in pulmonary adenocarcinoma detects; Serum alpha-fetoprotein (AFP) horizontal detection can diagnosing primary cell carcinoma judge lapsing to of disease; HCG (HCG) horizontal detection in pregnant woman urine can diagnose early pregnancy; Excrement calprotectin content (FCC) detects can diagnose inflammatory bowel; Carcinoembryonic antigen (CEA) in feces is the biomarker of gastrointestinal tumor, and its sensitivity even detects or fecal occult blood testing higher than patients serum CEA.Because the biomarker in feces is directly excreted to feces from intestinal mucosa secretion, the pathology of direct reaction intestinal mucosa, physiological status, so by detecting some specific biomarkers in feces, there is higher value to the diagnosis of some intestinal tract disease.
Although features such as stool examination has simply, noinvasive, cheapnesss, and Chang Zuowei disorder in screening or the means made a definite diagnosis.But its prerequisite is stool sample must be left and taken by strict operation, should be sent to appointed place immediately, then be transferred to laboratory or specimen center by medical personnel, check as early as possible after leaving and taking.But, in real work, because middle swivel link is numerous, the time that feces is expelled to sampling is different in size, more owing to being full of stink, collect and need to sample in urinal, often more difficult, the sampling mode of this shortage strict standard limits or has influence on reliability and the accuracy of check result greatly, in order to accurately detect the biomarker in sample,, improve specificity and the sensitivity of feces biomarker, need a kind of simple, effective and standardized sampling approach fast of exploitation.
Summary of the invention
Technical problem to be solved by this invention, is just to provide a kind of intestinal biomarker sampler, can easy, quick, safe, automatically the biomarker in Excreta in intestinal is sampled.
For solving the problems of the technologies described above, the present invention is achieved through the following technical solutions:
A kind of intestinal biomarker sampler, comprising: a shell, in capsule-type; One inner shell, is positioned at shell, is close to the shape adaptation of shell with outer casing inner wall; Multiple microsphere, is filled in inner shell, and the outer surface of described microsphere is with the ligands specific film be combined with outside biomarker; Adsorb the outside biomarker with its pairing by the ligands specific film of microsphere surface, realize the sampling of intestinal biomarker.
Further, described inner shell is water-soluble cellulose derivative, and microsphere both can have been protected not dissociate inner shell, target biology mark can be made again freely to be adsorbed with the part of microsphere surface by its loose porous structure and combine.For the inner shell of other polymeric material, the present invention can additionally distribute the aperture that the outside biomarker of multiple confessions passes through on the housing of described inner shell.And for ease of fast collecting inner shell with magnetic field force, inner shell can be magnetic.
Further, for ease of filling microsphere or take out microsphere in inner shell, described inner shell is divided into cap body and inner shell body, and described cap body is removably installed on inner shell body.
Further, for preventing shell from being dissolved under one's belt, the outer surface of described shell scribbles one deck enteric gelatin protecting film, and it can dissolve at enteral, and does not dissolve at gastric.
Compared with prior art, the present invention has following beneficial effect:
The present invention is shell, the capsule-type structure of inner shell and microsphere, by the protection of shell, can do not dissolved under one's belt, and be decomposed in intestinal (large intestine and small intestinal), the target biology mark in absorption intestinal Excreta is combined by the ligands specific film of microsphere surface, thus when Excreta is discharged outside human body, fast inner shell is searched out by magnetic field suction type or alternate manner, removable inner shell again, detect microsphere, realize easy to biomarker, fast, safety sampling, fully can adhere to the biomarker in intestinal simultaneously, thus improve the specificity and sensitivity that detect.
Accompanying drawing explanation
Fig. 1 is the planar structure partial schematic sectional view of sampler of the present invention;
Fig. 2 is the stereochemical structure partial schematic sectional view of sampler of the present invention;
Fig. 3 is the structural representation of microsphere of the present invention.
In figure: 1, shell, 11, enteric gelatin protecting film, 2, inner shell, 21, aperture, 22, cap body, 23, inner shell body, 3, microsphere, 4, ligands specific film.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail.
A kind of intestinal biomarker sampler as shown in Fig. 1 ~ 2, comprising: a shell 1, in capsule-type; One inner shell 2, is positioned at shell, also in capsule-type, namely with the shape adaptation of shell 1, and is close to shell 1 inwall; Multiple microsphere 3, is filled in inner shell 2, as shown in Figure 3, the outer surface of microsphere 3 with the ligands specific film 4 be combined with outside biomarker, namely biomarker can with ligand membrane specific binding.
Wherein, the outer surface of shell 1 scribbles one deck enteric gelatin protecting film 11, shell is not dissolved under one's belt, and dissolves in intestinal.Shell 1 dissolves rear thus exposes inner shell 2 in intestinal.The shell 1 of the present embodiment is about 0.8cm or shorter, and diameter is about 0.5mm or less, and volume is about 120mm
3.
As shown in Fig. 1 ~ 2, the inner shell 2 of the present embodiment is magnetic, also can be the housing of general polymer material, as fiber, plastics, plastic material.Inner shell also can be metal material, to determine the position of this sampler under being irradiated by X-ray instrument.Inner shell is not dissolved in intestinal; through Laser Processing aperture 21; aperture is less than 20 μm; form loose structure; both can splendid attire, protection microsphere 3; its larger volume is also beneficial to be found in Excreta, and this inner shell 2 can make again the free structure by its porous of target biomarker thus adsorb with the ligands specific film 4 on microsphere 3 surface to combine.In addition, inner shell 2 also can adopt water-soluble cellulose derivative material, itself has loose porous structure, is not dissolved in intestinal.
As shown in Fig. 1 ~ 2, inner shell 2 is divided into cap body 22 and inner shell body 23, and cap body 22 is removably installed on inner shell body 23, and the cap body 22 of the present embodiment and inner shell body 23 are provided with the Access Division of snap-fit engagement, can cover by convenient plug-in mounting.Open cap body 22, new microsphere 3 can be filled, also can take out with intestinal internal target biomarker specific binding after microsphere 3.
Screen absorption and the outside biomarker of its pairing by the ligands specific film 4 on microsphere 3 surface, realize the sampling of biomarker.After the inner shell 2 being loaded with microsphere 3 is discharged with Excreta, available magnetic field fast searching to its position, and is separated with Excreta.The ligands specific film 4 that microsphere 3 surface coverage adheres to, can be a-protein/G/L-immunoglobulin, Ag-Ab, it has stronger adhesion, the target biology mark in intestinal can be identified very soon, such as secretory IgA, sIgA, calprotectin, lactoferrin, pathogen antigen etc., then take out microsphere 3 SDS-PAGE, ELISA and protein groups technical Analysis, reflection disease symptoms.
In addition, microsphere 3 can coat and be different from other color excremental, is convenient to find; Also microsphere can be arranged to magnetic, be convenient to magnetic field force and find.Microsphere can be used to the antibody of covalent coupling biomarker simultaneously.Sampler of the present invention also can be used to the biomarker sampling of mouth, conjunctival sac and female genital tract.
Embodiments of the present invention are not limited thereto; according to foregoing of the present invention; utilize ordinary technical knowledge and the customary means of this area; do not departing under the present invention's above-mentioned basic fundamental thought prerequisite; the present invention can also make the amendment of other various ways, replacement or change, all drops within rights protection scope of the present invention.
Claims (7)
1. an intestinal biomarker sampler, is characterized in that, comprising:
One shell, in capsule-type;
One inner shell, is positioned at shell, is close to the shape adaptation of shell with outer casing inner wall;
Multiple microsphere, is filled in inner shell, and the outer surface of described microsphere is with the ligands specific film be combined with intestinal biomarker;
Adsorb the intestinal biomarker with its pairing by the ligands specific film of microsphere surface, realize the sampling of intestinal biomarker.
2. intestinal biomarker sampler according to claim 1, is characterized in that: described inner shell is water-soluble cellulose derivative.
3. intestinal biomarker sampler according to claim 1, is characterized in that: the housing of described inner shell is distributed with the aperture that the outside biomarker of multiple confession passes through.
4. intestinal biomarker sampler according to claim 3, is characterized in that: described inner shell is magnetic.
5. the intestinal biomarker sampler according to any one of claim 2-4, is characterized in that: described inner shell is divided into cap body and inner shell body, and described cap body is removably installed on inner shell body.
6. intestinal biomarker sampler according to claim 1, is characterized in that: the outer surface of described shell scribbles one deck enteric gelatin protecting film.
7. intestinal biomarker sampler according to claim 1, is characterized in that: described microsphere is magnetic.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310456050.6A CN103479401B (en) | 2013-09-29 | 2013-09-29 | A kind of intestinal biomarker sampler |
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| CN201310456050.6A CN103479401B (en) | 2013-09-29 | 2013-09-29 | A kind of intestinal biomarker sampler |
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| CN103479401A CN103479401A (en) | 2014-01-01 |
| CN103479401B true CN103479401B (en) | 2015-08-19 |
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| CN201310456050.6A Expired - Fee Related CN103479401B (en) | 2013-09-29 | 2013-09-29 | A kind of intestinal biomarker sampler |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110974311B (en) * | 2019-12-24 | 2022-08-30 | 华中科技大学鄂州工业技术研究院 | Intestinal microorganism collection capsule |
| CN114305520B (en) * | 2022-03-11 | 2022-05-13 | 中山大学附属第一医院 | Duodenum multistage secretion sampling device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102905753A (en) * | 2009-12-24 | 2013-01-30 | 因卡伯实验室有限责任公司 | Swallowable drug delivery device and methods of drug delivery |
| WO2013028548A2 (en) * | 2011-08-19 | 2013-02-28 | The Regents Of The University Of California | Compositions and devices for the detection of biomarkers in the gastrointestinal tract and methods for making and using them |
| CN103025890A (en) * | 2010-04-06 | 2013-04-03 | 卡里斯生命科学卢森堡控股 | Circulating biomarkers for disease |
| CN203506763U (en) * | 2013-09-29 | 2014-04-02 | 中山大学附属第一医院 | Intestinal tract biomarker sampler |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20050260137A1 (en) * | 2004-05-18 | 2005-11-24 | General Electric Company | Contrast agents for magnetic resonance imaging |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102905753A (en) * | 2009-12-24 | 2013-01-30 | 因卡伯实验室有限责任公司 | Swallowable drug delivery device and methods of drug delivery |
| CN103025890A (en) * | 2010-04-06 | 2013-04-03 | 卡里斯生命科学卢森堡控股 | Circulating biomarkers for disease |
| WO2013028548A2 (en) * | 2011-08-19 | 2013-02-28 | The Regents Of The University Of California | Compositions and devices for the detection of biomarkers in the gastrointestinal tract and methods for making and using them |
| CN203506763U (en) * | 2013-09-29 | 2014-04-02 | 中山大学附属第一医院 | Intestinal tract biomarker sampler |
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