CN103479351B - Electrophysiological recording device - Google Patents
Electrophysiological recording device Download PDFInfo
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- CN103479351B CN103479351B CN201310450212.5A CN201310450212A CN103479351B CN 103479351 B CN103479351 B CN 103479351B CN 201310450212 A CN201310450212 A CN 201310450212A CN 103479351 B CN103479351 B CN 103479351B
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Abstract
An electrophysiological recording device comprises a guide tube component, an electrode component and a sample feeding component. The guide tube component comprises a guide tube holding marking dye. The electrode component is used for acquiring the position of the marking dye and coupled to one end of the guide tube. The sample feeding component is connected with the other end of the guide tube and pushes the marking dye which is then guided out. The electrophysiological recording device is available for marking the position an electrode accurately.
Description
Technical field
The invention belongs to Electrophysiology technical field, particularly relate to a kind of electrophysiological recording device.
Background technology
Electrophysiology technology is research neuroscience, resolves the important tool of cerebral nerve network.For the patch clamp technique of classics, macroscopic view, it can study the neuronal connections in two brain intervals; Microcosmic, it can record the activity of single ion channel, makes people reach the unthinkable stage of forefathers to neuronic understanding.But nervous system completes in hundreds of millions neuronic specific connections and coded system collaborate.Therefore the understanding of whole nervous system and brain function is also known little about it.Along with going deep into of studying the working mechanism of neutral net, people make up the deficiency of existing electrophysiological recording technology in the urgent need to new method.
Relative to the patch-clamp electrophysiological technique of classics, multichannel electrophysiological recording technology adopts the method for extracellular recording to monitor multiple neuronic synchronized discharge in the middle of neuron pool.Applying this method can a large amount of neuronic electrical activity in synchronous recording multiple brain district, the electrophysiological function contact being convenient to study between Different brain region is even individual when accepting stimulate or perform a certain specific behavior task, the Neural spike train contact over time and space of Different brain region, and then the working mechanism of neutral net in brain is studied by analyzing neuronic discharge mode.Compared with patch clamp technique, its advantage is on live body, to record the more huge electrical activity of neurons of quantity.But Patch-clamp techniques system can use with micro imaging system usually jointly, utilizes fluorescent labelling techniques, can optionally study the neuron of ad-hoc location and type.And electrode is implanted to target location by the method that multiple recording technology is located by brain map usually goes forward side by side line item.But owing to there is the factors such as individual variation, in experiment, the brain district of practical study may exist deviation with target area, the electrical activity of neurons that physical record arrives may not from target core group.
The uncertain of this electrophysiological recording position just may bring some errors even information of mistake in the middle of research, allows an experimental result need more work repeatedly to confirm, greatly increases workload.Even because the deviation of position, may get the wrong sow by the ear.In order to address this problem, current people can, after electrophysiological recording terminates, adopt the method for brain sheet tissue staining to verify.
Use multichannel in the middle of the research of body electrophysiological recording in many needs, target location is just given out by people, and the mode then repeatedly verified by many experiments gets rid of as far as possible because the inaccurate adverse effect caused of record position.This method not only increases the workload of experimentation, and can not shield the result error that possible position deviation brings completely.
And by the method for carrying out tissue staining after laboratory animal record and confirming, in fact there is very large breach, especially anaesthetize electrophysiological recording.From cerebral tissue, being taken out to dyeing from electrode also has a lot to operation and a very long time, there is a lot of probabilities in the process, and such as electrode takes out may have other damage to cerebral tissue; Cerebral tissue is flexible, gathering after electrode takes out of can do by myself; The damage that in short time, electrode pair cerebral tissue causes is large not, can not well be identified even if dyeed; The direction of section and the track of bottom electrode not in one plane, as long as a little deviation just can not see complete electrode vestige, can only judge that at most whether the direction of bottom electrode is correct, and be difficult to the position judging eletrode tip.
Summary of the invention
Based on this, be necessary to provide a kind of can the electrophysiological recording device of labelling electrode position comparatively accurately.
A kind of electrophysiological recording device, comprising:
Conduit tube component, comprises conduit, has labeling dye in described conduit;
Electrode assemblie, for gathering the position of labeling dye, the coupled one end of described electrode assemblie and described conduit; And
Sample introduction module, is connected with the other end of described conduit, and provides a motive force to derive to impel described labeling dye to described labeling dye.
Further, described conduit is silicone tube, glass tubing or poly property management; The external diameter of described conduit is 20 microns ~ 500 microns; Described labeling dye is CNT, quantum dot or immunofluorescent stains.
Further, described sample introduction module comprises syringe, and described syringe has injection needle, and described conduit is sheathed on described injection needle away from one end of described electrode assemblie.
Further, described sample introduction module also comprises the syringe pump adding and be held on described syringe.
Further, described conduit tube component also comprises the connection tube that one end and described conduit are socketed away from one end of described electrode assemblie, and the other end of described connection tube is sheathed on described injection needle.
Further, described connection tube is plastic tube or rubber tube; Be provided with implant in described connection tube, described implant along described connection tube axially slidably; Described implant is inert fluid.
Further, described conduit tube component also comprises sealing shroud, and described sealing shroud is sheathed on the junction of described conduit and described connection tube and seals the junction of described conduit and described connection tube; The material of described sealing shroud is rubber, latex or silica gel.
Further, described electrode assemblie comprises multiple electrode be arranged in array, described multiple electrode all with the coupled one end of described conduit away from described sample introduction module, in described multiple electrode, at least one is recording electrode.
Further, fibre electrode is also had in described multiple electrode.
Further, stimulating electrode is also had in described multiple electrode.
Above-mentioned electrophysiological recording device comprises conduit tube component, electrode assemblie and sample introduction module, when using this electrophysiological recording device to carry out labelling to cerebral tissue, cerebral tissue is all inserted in one end of the conduit of electrode assemblie and conduit tube component, by sample introduction module, the labeling dye in conduit is derived, the electrode assemblie be coupled with conduit can gather the position of labeling dye, thus location records electrophysiological recording being terminated that time gets off, thus to determine the actual position of eletrode tip in electrophysiological recording experiment, therefore, above-mentioned electrophysiological recording device can the position of labelling electrode comparatively accurately.
Accompanying drawing explanation
Fig. 1 is the structural representation that the electrophysiological recording electrode of an embodiment eliminates electrode assemblie;
Fig. 2 is the electrode assemblie of electrophysiological recording electrode shown in Fig. 1 and the structural representation of conduit;
Fig. 3 is the structural representation of the conduit tube component of the electrophysiological recording electrode shown in Fig. 1;
Fig. 4 is the structural representation of the conduit of the electrophysiological recording electrode shown in Fig. 3;
The structural representation that Fig. 5 is the electrode assemblie of electrophysiological recording electrode shown in Fig. 2 and another angle of conduit.
Detailed description of the invention
In order to make object of the present invention, technical scheme and advantage more clear, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Refer to Fig. 1 and Fig. 2, the electrophysiological recording device 10 of an embodiment, comprise conduit tube component 100, electrode assemblie 200 and sample introduction module 300.
See also Fig. 3, conduit tube component 100 comprises conduit 110, has labeling dye 112 in conduit 110.As shown in Figure 4, conduit 110 is derived labeling dye 112.Conduit 110 can be the tube that material is comparatively solid and hard, and be preferably silicone tube, glass tubing or poly property management, wherein, poly property management can be polyurethane tube, polyfluortetraethylene pipe.The conduit of these materials is durable and hard, after can avoiding fractureing or entering cerebral tissue, cannot to be consistent target approach region, ground because of bending with electrode, and the good leak tightness of the sidewall of the conduit of these materials, labeling dye 112 can not be made to leak.Further, in conduit 110, other dyestuff can also be stored, such as, not by the medicine that labeling dye 112 affects.The external diameter of conduit 110 is 20 microns ~ 500 microns.Wherein, the labeling dye 112 being not easy to spread and wash that labeling dye 112 can be commonly used for this area, be preferably CNT quantum dot or immunofluorescent stains, wherein, immunofluorescence label dyestuff 112 can have fluorescence to be maybe coupled the labeling dye 112 of " antigen " of other band fluorescence for its own band; And the color of labeling dye 112 can be black, or other color.Further, conduit tube component 100 also comprises the connection tube 120 that one end and conduit 110 are socketed away from one end of electrode assemblie 200.Connection tube 120 is the good long tube of pliability.Connection tube 120 is plastic tube or rubber tube.Further, in connection tube 120, be provided with implant, this implant along connection tube 120 axially slidably.Wherein, implant is can the material of sealed joint pipe, is preferably inert fluid, such as, and paraffin oil, silicone oil.Further, conduit tube component 100 also comprises sealing shroud 130, and sealing shroud 130 is sheathed on the junction of conduit 110 and connection tube 120 and the junction of sealing duct 110 and connection tube 120.Can prevent the liquid in conduit tube component 100 from revealing from conduit 110 and the gap of the junction of connection tube 120 by arranging sealing shroud 130.Wherein, sealing shroud 130 is good and have elastic material for air-tightness, is preferably rubber, latex or silica gel.Be appreciated that, sealing shroud 130 can omit, and conduit 110 can also adopt alternate manner to seal with the junction of connection tube 120, such as, with the glue coating conduit 110 that can solidify with the gap of the junction of connection tube 120, with the junction of sealing duct 110 with connection tube 120; Or even connection tube 120 also can omit, now, conduit 110 is directly connected with sample introduction module 300 away from one end of electrode assemblie 200.
See also Fig. 2 and Fig. 5, electrode assemblie 200 is for gathering electricity physiological signal.Electrode 200 comprises multiple electrode 210 be arranged in array, multiple electrode 210 all with the coupled one end of conduit 110 away from sample introduction module 300.Wherein, in multiple electrode 210, at least one is recording electrode.Be appreciated that electrode assemblie 200 also can only include an electrode 210, and this electrode 200 is recording electrode.Preferably, also have fibre electrode in multiple electrode 210, by arranging fibre electrode, photostimulation being carried out to posting field, the function of photostimulation can be carried out posting field, thus recording light stimulates and the electrophysiological change of front and back.Further, in multiple electrode 210, going back stimulating electrode, by arranging stimulating electrode, electricity irritation being carried out to posting field, thus the neuron change of electricity irritation induction can be observed, as by excited or suppress, or change neuron coded system etc.Be appreciated that multiple electrode 210 can only have recording electrode and fibre electrode; Also recording electrode and stimulating electrode can be only had; Also can be have recording electrode, fibre electrode and stimulating electrode simultaneously; Or even, the electrode of recording electrode, fibre electrode, stimulating electrode and other function can also be had in multiple electrode 210 simultaneously, thus make this electrode assemblie 200 have more function.
Please again consult Fig. 1, sample introduction module 300 is connected with the other end of conduit 110, and provides a motive force to derive to impel labeling dye 112 to labeling dye 112.Sample introduction module 300 comprises syringe 310.Syringe 310 has injection needle 312, and conduit 110 is sheathed on injection needle 312 away from one end of electrode assemblie 200.In the particular embodiment, one end away from conduit 110 of connection tube 120 is sheathed on injection needle 312.Further, sample introduction module 300 also comprises the syringe pump 310 adding and be held on syringe 310.Syringe pump 310 can control syringe 310 with the derivation speed of control mark dyestuff 112 and total amount, thus the derivation of control mark dyestuff 112 more accurately.Wherein, syringe 310 is microsyringe, and syringe pump 320 is micro-injection pump, thus is more accurately derived to inject cerebral tissue by labeling dye 112 from conduit 110.Be appreciated that syringe pump 320 also can omit; In addition, sample introduction module 300 also can not take the mode of syringe 310, and alternate manner also can be adopted to be derived from conduit 110 by labeling dye 112.
Further, conduit tube component 100 also comprises the sealing member (not shown) of the junction of sealed joint pipe 120 and injection needle 312, thus well can seal the junction of connection tube 120 and injection needle 312.Be appreciated that sealing member can omit, the glue that can solidify can be adopted to be coated in the junction of connection tube 120 and injection needle 312.
Above-mentioned electrophysiological recording device 10 comprises conduit tube component 100, electrode assemblie 200 and sample introduction module 300, when using this electrophysiological recording device 10 pairs of cerebral tissue to carry out labelling, electrode assemblie 200 and one end of the conduit 110 of conduit tube component 100 are all inserted cerebral tissue, by sample introduction module 300, the labeling dye 112 in conduit 110 is derived, the electrode assemblie 200 be coupled with conduit 110 can gather the position of labeling dye 112, thus location records electrophysiological recording being terminated that time gets off, thus to determine the actual position of eletrode tip in electrophysiological recording experiment.Therefore, above-mentioned electrophysiological recording device 10 can the position of labelling electrode comparatively accurately.
The above, it is only preferred embodiment of the present invention, not any pro forma restriction is done to the present invention, although the present invention discloses as above with preferred embodiment, but and be not used to limit the present invention, any those skilled in the art, do not departing within the scope of technical solution of the present invention, make a little change when the technology contents of above-mentioned announcement can be utilized or be modified to the Equivalent embodiments of equivalent variations, in every case be do not depart from technical solution of the present invention content, according to any simple modification that technical spirit of the present invention is done above embodiment, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (6)
1. an electrophysiological recording device, is characterized in that, comprising:
Conduit tube component, comprises conduit, has labeling dye in described conduit;
Electrode assemblie, for gathering the position of labeling dye, the coupled one end of described electrode assemblie and described conduit; And
Sample introduction module, be connected with the other end of described conduit, and provide a motive force to derive to impel described labeling dye to described labeling dye, described sample introduction module comprises syringe, described syringe has injection needle, described conduit is sheathed on described injection needle away from one end of described electrode assemblie, and described sample introduction module also comprises the syringe pump adding and be held on described syringe;
Described conduit tube component also comprises the connection tube that one end and described conduit are socketed away from one end of described electrode assemblie, the other end of described connection tube is sheathed on described injection needle, described conduit tube component also comprises sealing shroud, and described sealing shroud is sheathed on the junction of described conduit and described connection tube and seals the junction of described conduit and described connection tube;
Described electrode assemblie comprises multiple electrode be arranged in array, described multiple electrode all with the coupled one end of described conduit away from described sample introduction module, in described multiple electrode, at least one is recording electrode.
2. electrophysiological recording device according to claim 1, is characterized in that, described conduit is silicone tube, glass tubing or poly property management; The external diameter of described conduit is 20 microns ~ 500 microns; Described labeling dye is CNT, quantum dot or immunofluorescent stains.
3. electrophysiological recording device according to claim 1, is characterized in that, described connection tube is plastic tube or rubber tube; Be provided with implant in described connection tube, described implant along described connection tube axially slidably; Described implant is inert fluid.
4. electrophysiological recording device according to claim 1, is characterized in that, the material of described sealing shroud is rubber, latex or silica gel.
5. electrophysiological recording device according to claim 1, is characterized in that, also has fibre electrode in described multiple electrode.
6. electrophysiological recording device according to claim 1, is characterized in that, also has stimulating electrode in described multiple electrode.
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| CN201310450212.5A CN103479351B (en) | 2013-09-27 | 2013-09-27 | Electrophysiological recording device |
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| CN201310450212.5A CN103479351B (en) | 2013-09-27 | 2013-09-27 | Electrophysiological recording device |
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| CN103479351B true CN103479351B (en) | 2015-06-10 |
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| CN109394211B (en) * | 2018-12-18 | 2021-08-06 | 新乡医学院 | Method for preparing in-vivo electrophysiological recording metal electrode |
| CN109394202A (en) * | 2018-12-24 | 2019-03-01 | 浙江中医药大学 | Nerve stimulation record component and preparation method thereof |
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| CN1836650A (en) * | 2006-04-19 | 2006-09-27 | 吉林省一心制药有限公司 | Antineoplastic precharging preparation |
| CN1978566A (en) * | 2005-12-08 | 2007-06-13 | 上海康德莱企业发展集团有限公司 | Needle head siliconizing keagent, and its siliconizing method |
| CN203208464U (en) * | 2013-04-15 | 2013-09-25 | 段安丽 | Two-cavity both-way injection needle device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US7455666B2 (en) * | 2001-07-13 | 2008-11-25 | Board Of Regents, The University Of Texas System | Methods and apparatuses for navigating the subarachnoid space |
| US20050283148A1 (en) * | 2004-06-17 | 2005-12-22 | Janssen William M | Ablation apparatus and system to limit nerve conduction |
| US20090254134A1 (en) * | 2008-02-04 | 2009-10-08 | Medtrode Inc. | Hybrid ultrasound/electrode device for neural stimulation and recording |
| US20110245662A1 (en) * | 2010-04-06 | 2011-10-06 | Eggers Philip E | Hemodynamic Detection of Circulatory Anomalies |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1499520A (en) * | 1974-03-01 | 1978-02-01 | Wilson B | Kinetic memory electrodes catheters and cannulae |
| CN1978566A (en) * | 2005-12-08 | 2007-06-13 | 上海康德莱企业发展集团有限公司 | Needle head siliconizing keagent, and its siliconizing method |
| CN1836650A (en) * | 2006-04-19 | 2006-09-27 | 吉林省一心制药有限公司 | Antineoplastic precharging preparation |
| CN203208464U (en) * | 2013-04-15 | 2013-09-25 | 段安丽 | Two-cavity both-way injection needle device |
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