CN103478401B - Method of producing bean pulp hydrolysate in enzyme-acid method - Google Patents
Method of producing bean pulp hydrolysate in enzyme-acid method Download PDFInfo
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- CN103478401B CN103478401B CN201310413434.XA CN201310413434A CN103478401B CN 103478401 B CN103478401 B CN 103478401B CN 201310413434 A CN201310413434 A CN 201310413434A CN 103478401 B CN103478401 B CN 103478401B
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Abstract
The invention relates to the technical field of bioengineering, and particularly discloses a method of producing bean pulp hydrolysate in an enzyme-acid method. The method of producing the bean pulp hydrolysate in the enzyme-acid method makes bean pulp as raw material and is characterized in that water is added to the bean pulp, temperature is controlled, and emulsification is conducted; the temperature is lowered, the pH value is adjusted, heat preservation is conducted, and enzymolysis is conducted; acid is added, and the temperature is raised; the temperature is controlled, pressure is kept, and acidolysis is conducted; the bean pulp hydrolysate is obtained after temperature lowering and filtering. According to the method of producing the bean pulp hydrolysate in the enzyme-acid method, proteolysis is complete, the reaction condition is moderate, controllability is strong, damage to amino acid is low, the bean pulp hydrolysate is golden yellow, the yield is high, quality is good, the content of amino nitrogen, total nitrogen and available nitrogen is high, and the method of producing the bean pulp hydrolysate in the enzyme-acid method is suitable for being popularized and applied widely.
Description
(1) technical field
The present invention relates to technical field of bioengineering, particularly a kind of enzymatic acidification method produces the method for soybean meal hydrolysate.
(2) background technology
Soybean meal hydrolysate, another name beans are dense, are obtained through being hydrolyzed by defatted soybean meal.It contains abundant amino acid, nucleic acid, vitamin and inorganic salts etc., is fermentable industrial production as the important source material of liquid organic nitrogen source and biotin.
Dregs of beans contains the crude protein of 40-44%, and therefore its purposes is quite extensive, can be directly used in and produce edible bean powder, adopts production method hydrolysis dregs of beans protein product (being commonly called as soybean meal hydrolysate) of strong acid, high concentration, high temperature, pressurization.
Soybean meal hydrolysate, its different production method causes the widely different of recovery rate height, and product quality also has very large great disparity, directly affects yield and the quality of fermentable product.At present, the method for China's production soybean meal hydrolysate is divided into acid hydrolyzation and the large class of enzymatic isolation method two.
Acid hydrolyzation process is: dregs of beans → batching → emulsification → heat-insulation pressure keeping hydrolysis → cooling → filtration → finished product; Enzymatic isolation method process is: dregs of beans → add water → emulsification → tune pH → enzyme-added → hydrolysis → filtration → finished product.
Acid hydrolyzation usually with 5-10 20% sour boiling reflux 16-20h doubly, or stresses on 120 degrees Celsius of hydrolysis 12h, proteolysis can be become amino acid.Its advantage is for being hydrolyzed thoroughly, and the end product of hydrolysis is L-amino acid, does not have the generation of optical isomer, yield height 2.0-2.2t/t, and amino nitrogen content can reach 2.3-2.5g/100ml, and total nitrogen content can reach 3.2-3.4g/100ml; Shortcoming is that requirement equipment has stronger corrosion resistance, and be that to carry out reaction condition at high temperature under high pressure violent, controllability is low, certain destruction and mutarotation can be caused to amino acid, acid hydrolysis simultaneously can produce some carcinogens, the tryptophan that nutritive value is higher is almost all destroyed, the serine of hydroxyl, threonine, TYR also have part destroyed, and generate a kind of atrament with acting on containing the compound (as sugar) of aldehyde radical, become rotten black substance, therefore hydrolyzate is black, and finished color is dark, and available nitrogen only has 70%.
The reaction condition of enzymatic isolation method is normal temperature and pressure and pH value is 2-8.Its advantage is mild condition, and amino acid is not destroyed completely, optical isomerism does not occur.Do not produce rotten black substance, therefore hydrolyzate is in light color, and finished color is golden yellow, available nitrogen 80-85%; Shortcoming is hydrolyzed thoroughly, and intermediate product is more, and dividing according to the degree of hydrolysis has protein-fat-peptone-polypeptide-dipeptides-amino acid, and the low 1.7-2.0t/t of yield, amino nitrogen content can reach 0.2-0.5g/ml, and total nitrogen content can reach 1.0-1.5g/100ml.
(3) summary of the invention
The present invention in order to make up the deficiencies in the prior art, provide a kind ofly to be hydrolyzed thoroughly, reaction condition is gentle, controllability is strong, amino acid destroys low enzymatic acidification method production soybean meal hydrolysate method.
The present invention is achieved through the following technical solutions:
Enzymatic acidification method produces a method for soybean meal hydrolysate, take dregs of beans as raw material, it is characterized in that: add water dregs of beans temperature control emulsification, and lower the temperature and adjust pH to be incubated enzymolysis, acid adding heats up, temperature control pressurize acidolysis, and obtain finished product through cooling, filtration, its concrete steps are as follows:
(1) dregs of beans is pulverized, and the water adding 2-3 times of volume is made into material liquid, is warming up to 80-90 DEG C, insulation 3-5h;
(2) be cooled to 40-45 DEG C, adjust pH to 5.0, add the papain accounting for material liquid weight 0.2-0.5%, at 45 DEG C, be hydrolyzed 60min;
(3) be warming up to 55 DEG C, adjust material liquid pH8.0, add the dregs of beans hydrolysis specific enzyme accounting for material liquid weight 0.3-0.6%, insulation 6h;
(4) be cooled to 30 DEG C, under stirring, slowly drip the sulfuric acid of 0.2-0.4 times of dregs of beans weight;
(5) 95-105 DEG C is warming up to, insulation acidolysis 17-20h;
(6) be cooled to 25-30 DEG C, filter to obtain finished product.
More excellent technical scheme of the present invention is:
In step (1), after described dregs of beans is pulverized, add the water of 2.5 times of volumes, be warming up to 85 DEG C, insulation 5h.
In step (2), be cooled to 42 DEG C, adjust pH to 5.0, add the papain accounting for material liquid weight 0.3%.
In step (3), the addition of dregs of beans hydrolysis specific enzyme is 0.3% of material liquid weight.
In step (4), the addition of sulfuric acid is 0.3 times of dregs of beans weight.
In step (5), be warming up to 105 DEG C, insulation acidolysis 20h.
In step (6), be cooled to 25 DEG C, filter to obtain finished product.
The technological process of production of the present invention is:
Dregs of beans → pulverizing → emulsification → tune PH → enzymolysis → acidolysis → cooling → filtration → finished product.
The product amino nitrogen content finally obtained can reach 3.3-3.5g/100ml, total nitrogen content can reach 4.5-4.8g/100ml, and product appearance is golden yellow, yield brings up to 2.4-2.6t/t by original 2.0-2.2t/t simultaneously, and available nitrogen brings up to 92-95% by original 80-85%.
Thoroughly, reaction condition is gentle in present protein hydrolysis, and controllability is strong, and amino acid destroys low, and product is golden yellow, and yield is high, quality good, and ammonia nitrogen, total nitrogen, available nitrogen content are high, are suitable for wide popularization and application.
(4) detailed description of the invention
Embodiment 1:
(1) dregs of beans is pulverized, and adds the water of 2.0 times, heats up 80 DEG C, be incubated 3 hours;
(2) be cooled to 40 DEG C, adjust PH to 5.0, add papain 0.2%, temperature 40 DEG C hydrolysis 60min;
(3) warming temperature 55 DEG C, under adjusting pH8.0 condition, adds the dregs of beans hydrolysis specific enzyme insulation 6h of 0.4%;
(4) sulfuric acid that 30 DEG C slowly drip dregs of beans amount 0.2 times is under agitation cooled to;
(5) 95 DEG C are warming up to, insulation acidolysis 20 hours;
(6) be cooled to 28 DEG C, filter to obtain finished product, finished product amino nitrogen content 3.3g/100ml, total nitrogen content can reach 4.5g/100ml, and product appearance is golden yellow, and yield is 2.4t/t simultaneously, and available nitrogen is 93%.
Embodiment 2:
(1) dregs of beans is pulverized, and adds the water of 2.5 times, heats up 85 DEG C, be incubated 5 hours;
(2) be cooled to 42 DEG C, adjust PH to 5.0, add papain 0.3%, temperature 45 C hydrolysis 60min;
(3) warming temperature 55 DEG C, under adjusting pH8.0 condition, adds guarantor's dregs of beans hydrolysis specific enzyme temperature 6h of 0.3%;
(4) sulfuric acid that 30 DEG C slowly drip dregs of beans amount 0.2 times is under agitation cooled to;
(5) 105 DEG C are warming up to, insulation acidolysis 20 hours;
(6) be cooled to 25 DEG C, filter to obtain finished product, finished product amino nitrogen content 3.5g/100m, total nitrogen content can reach 4.8g/100ml, and product appearance is golden yellow, yield 2.6t/t simultaneously, available nitrogen 95%.
Embodiment 3:
(1) dregs of beans is pulverized, and adds the water of 3 times, heats up 90 DEG C, be incubated 4 hours;
(2) be cooled to 45 DEG C, adjust PH to 5.0, add papain 0.5%, temperature 45 C, hydrolysis 60min;
(3) warming temperature 55 DEG C, under adjusting pH8.0 condition, adds the dregs of beans hydrolysis specific enzyme insulation 6h of 0.6%;
(4) sulfuric acid that 30 DEG C slowly drip dregs of beans amount 0.4 times is under agitation cooled to;
(5) 100 DEG C are warming up to, insulation acidolysis 18 hours;
(6) be cooled to 30 DEG C, filter to obtain finished product, finished product amino nitrogen content 3.4g/100ml, total nitrogen content can reach 4.7g/100ml, and product appearance is golden yellow, yield 2.5t/t simultaneously, available nitrogen 94%.
The present invention all can pass through existing techniques in realizing without the technical characteristic described, and does not repeat them here.
Claims (5)
1. the method for an enzymatic acidification method production soybean meal hydrolysate, take dregs of beans as raw material, it is characterized in that: add water dregs of beans temperature control emulsification, cooling adjusts pH to be incubated enzymolysis, and acid adding heats up, temperature control pressurize acidolysis, obtain finished product through cooling, filtration, its concrete steps are as follows: (1) dregs of beans is pulverized, and the water adding 2.5 times of volumes is made into material liquid, be warming up to 85 DEG C, insulation 5h; (2) be cooled to 42 DEG C, adjust pH to 5.0, add the papain accounting for material liquid weight 0.3%, at 45 DEG C, be hydrolyzed 60min; (3) be warming up to 55 DEG C, adjust material liquid pH8.0, add the dregs of beans hydrolysis specific enzyme accounting for material liquid weight 0.3-0.6%, insulation 6h; (4) be cooled to 30 DEG C, under stirring, slowly drip the sulfuric acid of 0.2-0.4 times of dregs of beans weight; (5) 95-105 DEG C is warming up to, insulation acidolysis 17-20h; (6) be cooled to 25-30 DEG C, filter to obtain finished product.
2. enzymatic acidification method according to claim 1 produces the method for soybean meal hydrolysate, it is characterized in that: in step (3), and the addition of dregs of beans hydrolysis specific enzyme is 0.3% of material liquid weight.
3. enzymatic acidification method according to claim 1 produces the method for soybean meal hydrolysate, and it is characterized in that: in step (4), the addition of sulfuric acid is 0.3 times of dregs of beans weight.
4. enzymatic acidification method according to claim 1 produces the method for soybean meal hydrolysate, it is characterized in that: in step (5), be warming up to 105 DEG C, insulation acidolysis 20h.
5. enzymatic acidification method according to claim 1 produces the method for soybean meal hydrolysate, it is characterized in that: in step (6), be cooled to 25 DEG C, filter to obtain finished product.
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Denomination of invention: Method of producing bean pulp hydrolysate in enzyme-acid method Effective date of registration: 20191122 Granted publication date: 20150107 Pledgee: Shandong juancheng Rural Commercial Bank Co., Ltd Pledgor: Shandong Yangcheng Biotech Co., Ltd. Registration number: Y2019990000568 |
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Date of cancellation: 20201217 Granted publication date: 20150107 Pledgee: Shandong juancheng Rural Commercial Bank Co.,Ltd. Pledgor: SHANDONG YANGCHENG BIOTECH Co.,Ltd. Registration number: Y2019990000568 |
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Denomination of invention: A method of producing soybean meal hydrolysate by enzymatic acid method Effective date of registration: 20201217 Granted publication date: 20150107 Pledgee: Shandong juancheng Rural Commercial Bank Co.,Ltd. Plaza sub branch Pledgor: SHANDONG YANGCHENG BIOTECH Co.,Ltd. Registration number: Y2020990001443 |