CN103468716B - The paddy rice grain length new gene qGS3b of a kind of space mutagenesis generation and molecule labelling method thereof - Google Patents
The paddy rice grain length new gene qGS3b of a kind of space mutagenesis generation and molecule labelling method thereof Download PDFInfo
- Publication number
- CN103468716B CN103468716B CN201310401678.6A CN201310401678A CN103468716B CN 103468716 B CN103468716 B CN 103468716B CN 201310401678 A CN201310401678 A CN 201310401678A CN 103468716 B CN103468716 B CN 103468716B
- Authority
- CN
- China
- Prior art keywords
- breeding
- qgs3b
- grain
- grain length
- paddy rice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to one and increase paddy rice grain length and the new gene qGS3b of grain weight and molecule labelling method thereof simultaneously, belong to rice high yield quality breeding and molecular genetics field.Essence of the present invention is according to chain law of segregation, utilizes " big particle mutant " and the F of original parent land reclamation and cultivation 582Colony and derivative F thereof3Family, by One marker analysis, the grain length new gene qGS3b on the 3rd chromosome of location, it is thus achieved that the Applied economy phenotypic marker XM3 1 of the most closely linked PCR-based amplification.Apply the present invention to Appearance Quality of Paddy Rice and the assisted selection of yield and pyramiding breeding, can effectively make up the deficiency of conventional natural variation gene, in seedling stage, the breeding population of low generation can be carried out genotype selection, obtain exterior quality and the more preferable breeding material of Yield Components, save the process that Adult plant seed is identified, improve breeding efficiency, accelerate breeding process.
Description
Technical field
The present invention relates to one and increase paddy rice grain length and the new gene qGS3b of grain weight and molecule labelling method thereof simultaneously, belong to paddy rice
High yield and high quality breeding and molecular genetics field, it is adaptable in rice high yield quality breeding, introduce new grain length gene qGS3b,
And utilize molecular labeling to carry out exterior quality and the assisted selection of yield and pyramiding breeding.
Background technology
Grain type especially grain length (GL) is important Appearance Quality of Paddy Rice compositing factor, and grain weight (TGW) is then important yield
One of component.It is known that grain length profit grain is heavily affected by more than 100 gene/QTL site, there is quilt on a small quantity
Fine location and/or clone, be mainly distributed on the 2nd chromosome (gw2), the 3rd chromosome (GS3 and GL3.1), the 5th dye
Colour solid (qSW5/GW5), the 7th chromosome (GS7 and qSS7) and the 8th chromosome (GW8).But on these sites
Favorable allels derive from natural mutation, thus the utilization in molecular breeding is necessarily limited to.
Induced-mutation technique can be used to Widening genetic resource base and produce new hereditary variation.Compared with man-made radiation, space lures
Although it is relatively low to become mutation rate, but the efficiency obtaining favorable variation is the most of a relatively high.Along with developing rapidly of space technology, especially
Being the progress of China's recoverable lift-launch technology, the vegetable seeds of up to 21 batches has been sent into the near-earth rail of about 400km by China
Road, it is thus achieved that substantial amounts of variation.Although the hereditary basis producing these variations is the most clear, but some favourable variations
In breeding, demonstrated its value.
Land-reclaimable 58 is to be bred as by Japan, and introduces the japonica rice variety of China in nineteen sixties, and its accumulative popularizing area reaches
To more than 140 ten thousand mu, it is that China japonica rice educates one of sharp important parent.1988, people were from No. 8885 retrievable satellites of China
Having selected big particle mutant strain in land reclamation and cultivation 58 offspring carried, its seed is elongated, and the length of 3 grain is accumulative up to 3 cun,
Have the title of " three cun ", and savory, be to improve me to store up japonica rice and educate the excellent donor of profit rice quality.2002, this
Seminar is found by preliminary genetic research, and its type proterties is at the F of " big particle mutant "/land-reclaimable 582Colony just presents
State is distributed, it is likely that controlled by two or more site.
Present invention finds a main effect site qGS3b being positioned on the 3rd chromosome, explain that the hereditary variation of grain length and grain weight divides
Do not reach 15.9% and 10.9%, and obtain closely linked genetic marker XM3-1, be expected to be used for the mark auxiliary of new gene
Selection and use.The research utilizing rice sequences reference collection of illustrative plates and forefathers compares makees graph discovery, and this site not yet has control
The gene of grain type is found, and therefore qGS3b is a new gene controlling length and grain weight.
Summary of the invention
(1) technical problem
The present invention is directed to the studies above background, utilize " big particle mutant " and the F of original parent land reclamation and cultivation 582Colony and derivative
F3Family, by One marker analysis, the grain length grain gene qGS3b again on the 3rd chromosome of location, it is thus achieved that the most closely connect
The Applied economy phenotypic marker XM3-1 of the PCR-based of lock, this mark can effectively carry out grain length and the assisted Selection of grain weight, main
Appearance Quality of Paddy Rice to be applied to and the assisted selection of yield and pyramiding breeding.
(2) technical scheme
Paddy rice grain length new gene qGS3b, it is characterised in that: at rice genome the 3rd chromosome 34,000,000-37,000,000bp
Interval in there is a new gene site qGS3b closed with paddy grain length and grain heavy phase, " big grain is prominent for Flight Mutagenesis kind
Variant " allele on this site can dramatically increase grain length.
The molecule labelling method of qGS3b, it is characterised in that: by a pair special PCR primer to XM3-1, wherein forward primer
Sequence is: AGCGACGGATGCATGATC, and reverse primer sequences is: TTGAGCCGGAGGTAGTCTTG, altogether
With PCR amplification with kind " big particle mutant " blood relationship educate Piao material genomic DNA, if primer can to XM3-1
PCR amplifies the fragment of about the 105bp size similar with kind " big particle mutant ", then speculate that this breeding material very may be used
Can be containing the long grain allele of qGS3b.
Paddy rice grain length new gene qGS3b and molecule labelling method thereof can apply to rice breeding.
(3) beneficial effect
Identify the paddy rice grain length grain gene qGS3b again on the 3rd chromosome by the present invention and it can be carried out genotype mirror
Other codominant marker.
The present invention compared with prior art has the following advantages and effect:
1. it is the new gene produced from Flight Mutagenesis due to the long grain allele on qGS3b gene loci, it is possible to effective
Make up the deficiency of natural variation gene.
2. the screening marked by new gene, it is possible to for paddy grain length and grain weight gene pyramiding breeding, it is thus achieved that exterior quality and
The more preferable breeding material of Yield Components.
3. the molecular labeling of the present invention is owing to can carry out genotype selection in seedling stage to the breeding population of low generation, retains excellent
Body hybridizes further, can save the process that Adult plant seed is identified, accelerates breeding process.
Accompanying drawing explanation
The pcr amplification product of SSR marker XM3-1 of F3 offspring's strain of Fig. 1 " big particle mutant "/land reclamation and cultivation 58 is 5% poly-third
(1-28 is the F randomly selected for the banding pattern collection of illustrative plates of acrylamide gel electrophoresis and the phenotype of correspondence thereof3For strain；M is DNA
Ladder；P1 is long grain parent；P2 is normal parents；A be 105bp, b be 110bp).
The F of Fig. 2 " big particle mutant "/land reclamation and cultivation 583Two kinds after the selecting according to SSR marker XM3-1 of offspring's strain isozygoty
The average grain length of genotype individuals.
Detailed description of the invention
Below in conjunction with being embodied as example, the present invention is expanded on further.Wherein method therefor is conventional method if no special instructions.
(1) location of qGS3b gene
1. material to be tested
The kind " big particle mutant " being derived from China's Flight Mutagenesis is utilized to build cross combination with original parent land-reclaimable 58, it is thus achieved that F1
Filial generation selfing obtains F2360 individual plants of offspring and derivative F thereof3Family.
2.DNA extracts, PCR expands and gel electrophoresis
With reference to the DNA extraction method of (2000) such as Temnykh, the representative individual plant of each strain is mixed extraction gene respectively
Group DNA.According to reference to collection of illustrative plates, choose 600 SSR marker being uniformly distributed in full-length genome, synthetic primer.With each
The genomic DNA of strain is that template is carried out, and polymerase chain (PCR) reacts.The product of PCR reaction passes through polyacrylamide
Amine gel electrophoresis separates, after Ethidum Eremide dyeing, and imaging under gel imaging system.With reference to the amplified band of parents, right
The banding pattern of offspring's strain carries out differentiating record.
3. One marker analysis
The genotype represented by amplified band according to offspring's strain, resets the result looked into as table using the grain type grain of its corresponding individual plant
Offset, utilizes the qtl analysis software developed by Wang Jiankang seminar of Institute of Crop Science, Chinese Academy of Agricultural Science
(ICIMapping V3.2, freeware) carries out data analysis, to grain length, grain is wide, grain is thick and grain the most respectively with marker gene
Type carries out One-way ANOVA (One-wayANOVA), and LOD remarkable threshold is set to 2.9.The main effect base that screening effect is bigger
Because of site.
We detect one and paddy grain length grain heavy phase in the interval of the 34,000,000-37,000,000bp being positioned at the 3rd chromosome
The new gene site qGS3b closed, and obtain the most closely linked molecular labeling XM3-1 (table 1).This interval is the most still
The gene loci or the QTL that do not have any of rice grain shape grain weight are in the news.
Table 1. utilizes the F of " big particle mutant "/land-reclaimable 582The genetic parameter of the qGs3b that crowd surveillance arrives
(2) F3Strain offspring analysis
We utilize the molecular labeling XM3-1 of acquisition, at F3The genotype carrying out XM3-1 in strain offspring selects.Select respectively
Take XM3-1 labeled primer amplification banding pattern and " big particle mutant " and land-reclaimable 58 identical types of isozygotying (accompanying drawing 1), and difference
Measure its grain length.Found that mark the grain length of " big particle mutant " the homozygous genotype offspring selected according to XM3-1
Really the land reclamation and cultivation 58 homozygous genotype offspring selected than XM3-1 mark significantly increases (accompanying drawing 2)；Illustrate to utilize the new base of qGS3b
It is effective that the molecular labeling XM3-1 of cause carries out the marker assisted selection of rice grain length.
Above-mentioned enforcement limits the present invention the most in any form.
Claims (1)
1. the molecule labelling method application in rice breeding, it is characterised in that the mark for rice grain length assists choosing
Selecting, described method includes, by a pair special PCR primer to XM3-1, wherein forward primer sequence is
AGCGACGGATGCATGATC, reverse primer sequences is TTGAGCCGGAGGTAGTCTTG, and common PCR expands
Increase the breeding material genomic DNA of kind " big particle mutant " offspring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310401678.6A CN103468716B (en) | 2013-09-06 | 2013-09-06 | The paddy rice grain length new gene qGS3b of a kind of space mutagenesis generation and molecule labelling method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310401678.6A CN103468716B (en) | 2013-09-06 | 2013-09-06 | The paddy rice grain length new gene qGS3b of a kind of space mutagenesis generation and molecule labelling method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103468716A CN103468716A (en) | 2013-12-25 |
| CN103468716B true CN103468716B (en) | 2016-09-07 |
Family
ID=49793757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310401678.6A Expired - Fee Related CN103468716B (en) | 2013-09-06 | 2013-09-06 | The paddy rice grain length new gene qGS3b of a kind of space mutagenesis generation and molecule labelling method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103468716B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105441457B (en) * | 2015-12-16 | 2019-04-16 | 中国农业科学院深圳生物育种创新研究院 | A kind of QTL molecule labelling method relevant to low nitrogen Rice under Condition mass of 1000 kernel |
-
2013
- 2013-09-06 CN CN201310401678.6A patent/CN103468716B/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| 水稻种子经卫星搭载后大粒型突变系的分子生物学分析;邢金鹏 等;《航天医学与医学工程》;19950515;第8卷(第2期);109-113 * |
| 空间诱变水稻大粒型突变体的遗传育种研究;徐建龙 等;《遗传》;20021231;第24卷(第4期);431-433 * |
| 空间诱变水稻大粒突变体粒长形状的基因定位;钱宇;《万方数据》;20061231;1-51 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103468716A (en) | 2013-12-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103740711B (en) | Indel marker linked with yellow flesh gene yf of cucmis sativus L. and application of Indel marker | |
| CN103421815B (en) | A wide new gene qGS5 of rice grain dland molecule marking method | |
| De Keyser et al. | Multipoint-likelihood maximization mapping on 4 segregating populations to achieve an integrated framework map for QTL analysis in pot azalea (Rhododendron simsii hybrids) | |
| Sharma et al. | Single primer amplification reaction (SPAR) reveals intra-specific natural variation in Prosopis cineraria (L.) Druce | |
| CN108374053A (en) | The molecular labeling in peanut kernel percent main effect QTL site and its application | |
| CN102174510A (en) | Method for constructing lichi core collection by using EST-SSR (Expresses Sequence Tag-Simple Sequence Repeat) molecular marker | |
| CN105420368A (en) | Method for constructing fingerprint of phaseolus vulgaris according to SSR (simple sequence repeat) molecular marker and application | |
| Salami et al. | Genetic diversity of maize accessions (Zea mays L.) cultivated from Benin using microsatellites markers | |
| Kanno et al. | Conversion of a male-specific RAPD marker into an STS marker in Asparagus officinalis L. | |
| CN104313018A (en) | Indel marker of cucumber fruit spiny gene ns and application thereof | |
| CN106591460B (en) | Method for identifying variety of 'Zhongcha 302' tea tree by SSR molecular marker and application | |
| CN107881256A (en) | For identifying single nucleotide polymorphism site, primer pair, kit and the application of Peach fruits hardship benevolence/sweet tea benevolence character | |
| CN102876790B (en) | Simple sequence repeat (SSR) marker polymerase chain reaction (PCR) reaction method applied to chrysanthemum and related species thereof universally | |
| CN103468716B (en) | The paddy rice grain length new gene qGS3b of a kind of space mutagenesis generation and molecule labelling method thereof | |
| CN101017151A (en) | Purity testing method for tomato hybrid based on PCR technology | |
| CN103468715B (en) | The Oryza sativa L. grain length new gene qGS3a of a kind of space mutagenesis generation and molecule labelling method thereof | |
| CN104004749B (en) | Chain SSR labeled primer and the application thereof of cucumber black fruit Ci Jiyin B | |
| CN103725785B (en) | Construction method and application of teak clone fingerprint spectrums | |
| CN108703067A (en) | A kind of quick breeding method of long breeding time Ornamental rice kind | |
| CN103468791B (en) | Molecular marker assisted method used for variety selection of cowpea variety with grain-filling resistance, and PCR primer set used in molecular marker assisted method | |
| Bi et al. | Genetic diversity of wild population of Pyropia haitanensis based on SSR analysis | |
| CN103966178B (en) | A kind of yellowish green leaf related protein of paddy rice and encoding gene thereof and application | |
| CN103937788B (en) | The SSR marker of Folium Cucumidis sativi look mutator gene v-1 and application thereof | |
| CN106755413A (en) | Nitrogen in Rice absorbs site qNUE6 and its molecule labelling method | |
| CN104762298A (en) | Rice seedling-stage salt-tolerant gene qST11 and molecular marker method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160907 Termination date: 20200906 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |