CN103468398A - Method for degumming soybean blended oil with immobilized phospholipase A2 - Google Patents
Method for degumming soybean blended oil with immobilized phospholipase A2 Download PDFInfo
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- CN103468398A CN103468398A CN2013103902027A CN201310390202A CN103468398A CN 103468398 A CN103468398 A CN 103468398A CN 2013103902027 A CN2013103902027 A CN 2013103902027A CN 201310390202 A CN201310390202 A CN 201310390202A CN 103468398 A CN103468398 A CN 103468398A
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Abstract
The invention provides a method for degumming soybean blended oil with immobilized phospholipase A2. The blended oil is preevaporated to increase the contact possibility between the oil and the immobilized enzyme, thereby reducing the inactivation of the immobilized enzyme. The method optimizes the optimum parameters of the reaction temperature, initial reaction pH, addition amount of the immobilized enzyme, reaction time and other degumming conditions. The immobilized phospholipase A2 is used for degumming the soybean blended oil, thereby enhancing the activity and stability of the enzyme in the reaction system, reducing the scaling and pipeline blockage caused by the precipitation of colloids (phospholipid and the like) in the oil. enhancing the utilization ratio of equipment, and lowering the oil refinement cost; besides, the phosphorus content in the final enzyme-method degummed soybean oil obtained by degumming the soybean blended oil with the immobilized phospholipase A2 can be obviously lowered to 11.56 mg/kg; the color of the obtained raw oil (Y35, R6.5, and Lovibond 25.4mm dispoable cuvettes) is better than the color of conventionally degummed oil (Y35, R7.6, and Lovibond 25.4mm dispoable cuvettes); and thus, the method has important meanings for oil refinement.
Description
Technical field
The present invention relates to a kind of grease processing technique field, be specifically related to a kind of immobilized phospholipase A that uses
2the method that mixing oil is come unstuck.
Background technology
Grease is the requisite of people's daily life, and it plays very important physiological function at human body, is one of energy of human life.The method of producing grease mainly comprises oil extraction by pressing and lixiviation process liquefaction, and the lixiviation process liquefaction with its oil yield, high and resulting dregs of rice quality is more excellent becomes the method for making oil that most of liquefaction enterprise is favored.From leacher, grease out and the mixed solution of solvent are called mixing oil, and mixing oil must pass through certain processing, make separated from solvent in grease out.
Fat degumming is intended to remove the phosphatide in grease, and the phosphatide in grease is divided into hydrated phospholipid and nonhydratable phosphatide, and hydrated phospholipid more easily removes, but not hydrated phospholipid need to be processed, and is removed after making it change into the material that wetting ability is stronger.Phospholipase A
2nonhydratable phosphatide can be hydrolyzed into to lysophospholipid, the wetting ability of lysophospholipid significantly strengthens, thereby can it be separated from grease by centrifugal method after adding the water mixing.
Traditional complete processing is that mixing oil is carried out after pre-treatment its evaporation and stripping, except after desolventizing more further to grease come unstuck, a series of refinings such as depickling, decolouring, deodorization, but during due to miscella evaporation, grease does not pass through degumming technology, cause colloid class material (the being mainly phosphatide) part in grease to be separated out, form fouling, blocking pipe in equipment, reduce the utilization ratio of equipment, increased unnecessary cost.The present invention and the inventive method before us: a kind of immobilized phospholipase A that uses
2the method of coming unstuck (application number: 201210257043.9) with by the refining crude oil of soybean by immobilized phospholipase (patent No.: 200710072549.1) compare, invented before mixing oil is evaporated it has been carried out to enzymatic degumming, because mixing oil has less density than grease, be conducive to the settlement separate of the materials such as colloid; In addition, the mixing oil viscosity is less, for the adsorptive power of some colors also less, therefore some colors in the process of coming unstuck, also can partly take out of, for follow-up decoloration process is made preparation.
Adopt immobilized phospholipase A
2before mixing oil is evaporated, it is carried out to enzymatic degumming, studied mixing the oil degumming condition, finally determine and use immobilized phospholipase A
2the optimal processing parameter that mixing oil is come unstuck.
Summary of the invention
The present invention separates out in order to solve colloid class material (the being mainly phosphatide) part leached in mixing oil, form fouling, blocking pipe in equipment, reduced the utilization ratio of equipment, increased the problem of unnecessary cost etc., and propose used immobilized phospholipase A before mixing oil is evaporated
2the method that mixing oil is come unstuck.Use immobilized phospholipase A
2the method that soybean miscella is come unstuck realizes by following steps: one, the prevapourising of mixing oil; Two, immobilized phospholipase A
2pretreated mixing oil is come unstuck; Three, impurity and colloid in mixing oil are removed in centrifugal, filtration; Four, the mixing oil after processing is evaporated and stripping, remove solvent and obtain crude oil of soybean.
Adopt immobilized phospholipase A
2the method that soybean miscella is come unstuck, the enzyme reusability after immobilization is good, and not yielding inactivation greatly reduces the cost of oil and fat refining; Immobilized phospholipase A
2soybean miscella is come unstuck, and the enzymatic degumming soybean oil phosphorus content finally obtained significantly reduces, and can be down to 11.56mg/kg; In addition, the crude oil color and luster obtained (Y35, R6.5, Lovibond 25.4mm cell) is better than the come unstuck color and luster of rear grease (Y35, R7.6, Lovibond 25.4mm cell) of routine, significant to oil and fat refining.
Embodiment
Embodiment one: use immobilized phospholipase A
2the method that soybean miscella is come unstuck realizes by following steps: under the condition that is, 0.06 ~ 0.08MPa by soybean miscella at 60 ~ 70 ℃, vacuum tightness, prevapourising, to 40 ~ 65% of former weight, obtains the mixing oil after prevapourising; Two, immobilized phospholipase A
2for soybean miscella, come unstuck: heating soybean miscella to 60 ℃, add 45% citric acid solution of 0.1% left and right carry out acid-respons and cool to certain temperature, add the NaOH solution of 4% left and right to regulate initial pH to certain value, inwardly add hot soft water appropriate, with a certain amount of immobilized phospholipase A
2carry out the enzyme reaction certain hour, it is the enzymatic degumming mixing oil that the degummed oil that obtains adopts disk centrifugal separator (explosion-proof) to carry out after centrifugal 20min preserving the upper strata oil sample to it; Three, immobilized phospholipase A
2reclaim: reacted immobilized phospholipase A will come unstuck
2filtered and recycled; Four, the come unstuck phosphorus content of mixing oil crude oil after evaporation and stripping and the mensuration of color and luster after enzymatic degumming: in oil, the mensuration of phosphorus content adopts molybdenum blue colorimetric method, and the mensuration of color and luster adopts the Lovibond colorimetry; Five, enzyme process mixing oil the determining of optimal processing parameter that come unstuck: coming unstuck 35 ~ 55 ℃ of temperature respectively, initial pH is 4.0 ~ 8.0, and the immobilized enzyme addition is 0.05 ~ 0.25g/kg, under the condition that usually time is 2h ~ 8h, by mensuration immobilized phospholipase A
2the phosphorus content of the degummed oil obtained after soybean oil is come unstuck, draw and use immobilized phospholipase A
2the optimal conditions come unstuck; Six, the mixing oil after processing is carried out to flash distillation under 65 ~ 75 ℃, rising temperature to 110 ~ 115 ℃ are evaporated, and finally it are warmed up to 115 ~ 120 ℃ and carry out the high-pressure saturated steam stripping, remove solvent and obtain crude oil of soybean.
Embodiment two: the difference of present embodiment and embodiment one temperature that is in step 5 to come unstuck is 45 ℃ and uses immobilized phospholipase A again
2soybean miscella is come unstuck.Other step is identical with embodiment one.
Embodiment three: the difference of present embodiment and embodiment one is in step 5 that regulating pH with the NaOH solution of 4% left and right 6.0 uses immobilized phospholipase A again
2soybean miscella is come unstuck.Other step is identical with embodiment one.
Embodiment four: the difference of present embodiment and embodiment one is in step 5 the immobilized phospholipase A added in degumming oil
2for 0.15g/kg uses immobilized phospholipase A again
2soybean miscella is come unstuck.Other step is identical with embodiment one.
Embodiment five: the difference of present embodiment and embodiment one is in step 5 that the enzymatic degumming reaction times is 7h, is come unstuck to mixing oil.Other step is identical with embodiment one.
Claims (5)
1. use immobilized phospholipase A for one kind
2the method that soybean miscella is come unstuck, is characterized in that adopting immobilized phospholipase A
2before mixing oil is evaporated, it being carried out to enzymatic degumming realizes by following steps:
Step 1: under the condition that is 0.06 ~ 0.08MPa at 60 ~ 70 ℃, vacuum tightness by soybean miscella, prevapourising is to 40 ~ 65% of former weight, obtain the mixing oil after prevapourising, heating soybean miscella to 60 ℃, add 45% citric acid solution of 0.1% left and right to carry out acid-respons and cool to 35 ~ 55 ℃, add the NaOH solution of 4% left and right to regulate initial pH to certain value, inwardly add hot soft water appropriate, with a certain amount of immobilized phospholipase A
2carry out the enzyme reaction certain hour, it is the enzymatic degumming mixing oil that the degummed oil that obtains adopts disk centrifugal separator (explosion-proof) to carry out after centrifugal 20min preserving oil sample to it, the mixing oil that comes unstuck after enzymatic degumming is carried out to flash distillation under 65 ~ 75 ℃, rising temperature to 110 ~ 115 ℃ are evaporated, finally it is warmed up to 115 ~ 120 ℃ and carries out the high-pressure saturated steam stripping, remove solvent and obtain crude oil of soybean, the oil sample obtained is carried out to phosphorus detection, phosphorus detection adopts molybdenum blue colorimetric method to carry out, take the phosphorus content the lowest as degumming effect the best, determine the reaction optimum temperuture of coming unstuck,
Step 2: selecting to regulate pH with the NaOH solution of 4% left and right is 4.0 ~ 8.0, and the same step 1 of process, determine the initial pH come unstuck;
Step 3: select the immobilized phospholipase A added
2be 0.05 ~ 0.25g/kg, the same step 1 of process, determine immobilized phospholipase A
2addition;
Step 4: the selection usually time is 2 ~ 8h, and the same step 1 of process, determine usually time.
2. a kind of immobilized phospholipase A that uses according to claim 1
2the method of coming unstuck, is characterized in that in step 1 45% the citric acid solution that adds 0.1% left and right of 60 ℃ is carried out to acid-respons and cools to 45 ℃.
3. a kind of immobilized phospholipase A that uses according to claim 1
2the method of coming unstuck, is characterized in that regulating pH with the NaOH solution of 4% left and right in step 2 is 6.0.
4. a kind of immobilized phospholipase A that uses according to claim 1
2the method of coming unstuck, is characterized in that in step 3 the immobilized phospholipase A added in degumming oil
2for 0.15g/kg.
5. a kind of immobilized phospholipase A that uses according to claim 1
2the method of coming unstuck, is characterized in that in step 4 that the enzymatic degumming reaction times is 7h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2013103902027A CN103468398A (en) | 2013-09-02 | 2013-09-02 | Method for degumming soybean blended oil with immobilized phospholipase A2 |
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| CN2013103902027A CN103468398A (en) | 2013-09-02 | 2013-09-02 | Method for degumming soybean blended oil with immobilized phospholipase A2 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104561215A (en) * | 2015-01-17 | 2015-04-29 | 东北农业大学 | Method for preparing plant sterol ester by using immobilized lipid acyltransferase |
| CN104629907A (en) * | 2015-01-21 | 2015-05-20 | 东北农业大学 | Method for degumming soybean crude oil by utilizing immobilized lipid acyltransferase |
| CN105132148A (en) * | 2015-07-07 | 2015-12-09 | 东北农业大学 | Method for purifying crude oil by using magnetic immobilized phosphatidase A2 |
| CN112251288A (en) * | 2020-08-28 | 2021-01-22 | 菏泽中禾健元生物科技有限公司 | High-efficiency enzymatic degumming process for edible oil |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399627A (en) * | 2011-09-26 | 2012-04-04 | 华南理工大学 | Improved vegetable oil enzymatic degumming method |
| CN102776066A (en) * | 2012-07-24 | 2012-11-14 | 东北农业大学 | Degumming method through immobilized phospholipase A2 |
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2013
- 2013-09-02 CN CN2013103902027A patent/CN103468398A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399627A (en) * | 2011-09-26 | 2012-04-04 | 华南理工大学 | Improved vegetable oil enzymatic degumming method |
| CN102776066A (en) * | 2012-07-24 | 2012-11-14 | 东北农业大学 | Degumming method through immobilized phospholipase A2 |
Non-Patent Citations (2)
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| 凌秀菊等: ""酶解法脱除大豆混合油中磷脂的工艺研究"", 《中国油脂》 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104561215A (en) * | 2015-01-17 | 2015-04-29 | 东北农业大学 | Method for preparing plant sterol ester by using immobilized lipid acyltransferase |
| CN104629907A (en) * | 2015-01-21 | 2015-05-20 | 东北农业大学 | Method for degumming soybean crude oil by utilizing immobilized lipid acyltransferase |
| CN105132148A (en) * | 2015-07-07 | 2015-12-09 | 东北农业大学 | Method for purifying crude oil by using magnetic immobilized phosphatidase A2 |
| CN112251288A (en) * | 2020-08-28 | 2021-01-22 | 菏泽中禾健元生物科技有限公司 | High-efficiency enzymatic degumming process for edible oil |
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Application publication date: 20131225 |