CN103461319A - Pressure displacement method applied in dehydration and degreasing of plasticized specimen - Google Patents
Pressure displacement method applied in dehydration and degreasing of plasticized specimen Download PDFInfo
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- CN103461319A CN103461319A CN2013103242968A CN201310324296A CN103461319A CN 103461319 A CN103461319 A CN 103461319A CN 2013103242968 A CN2013103242968 A CN 2013103242968A CN 201310324296 A CN201310324296 A CN 201310324296A CN 103461319 A CN103461319 A CN 103461319A
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Abstract
The invention provides a pressure displacement method applied in dehydration and degreasing of a plasticized specimen. The method mainly comprises the following steps: placing an organism from which a specimen is to be prepared into a specimen tank filled with acetone, then placing the specimen tank in a pressure vessel and sealing the pressure vessel; adjusting the pressure of a pressure-reducing valve assembly of the pressure vessel to 0.1 MPa, introducing compressed gas into the pressure vessel, automatically stopping introduction of the gas when the pressure in the pressure vessel reaches 0.1 MPa and maintaining the pressure; and opening the pressure vessel to measure values like the proportion and fat content of an acetone solution every day, wherein dehydration and degreasing of the specimen are finished in about 1 to 2 weeks. The method provided by the invention greatly improves production efficiency, allows requirements for dehydration and degreasing indexes of the specimen of the organism to be met in 1 to 2 weeks instead of original 4 to 6 months and has the advantages of low production cost, low acetone replacing frequency, a limit amount of volatilization and conservation of raw materials.
Description
Technical field
The present invention relates to a kind of preparation method of biological sample.
Background technology
When biology being made into to the plasticizing sample, it is exactly by the Fat and moisture in organism with acetone extraction out that one important program arranged.Current extracting method is that the biology of sample to be made is placed in the container that fills acetone under normal temperature, normal pressure and soaks, then measure the numerical value such as proportion, fat content of acetone soln every a period of time, when these numerical value no longer change, the extractability that is original acetone reaches capacity, the acetone that need to more renew, and then the numerical value such as the proportion of periodic measurement acetone soln, fat content.In critical field and fat content consecutive numbers sunset while changing, the requirement that touched the mark of the interior Fat and moisture content of biological sample be described when acetone concentration.The weak point of said method is: 1, fabrication cycle is long, generally needs 4-6 month, and production efficiency is low; 2, need for a long time personnel tracking management, waste of manpower; 3, acetone needs often to change, and adds its volatilization, waste very greatly, and production cost is remained high; 4, it is helpless the biological sample of some urgent needs being made.
Summary of the invention
The object of the present invention is to provide that a kind of biological sample degreasing dewatering time is short, production cost is low, management is simple, save manpower apply to plastify the pressure displacement method in sample dewatering and defatting process.The present invention is placed in the Fat and moisture in it of pressurised extraction in pressure vessel by being immersed in biology in acetone.
Method of the present invention is specific as follows:
1, biology that will sample be made is placed in the sample groove of interior Sheng acetone;
2, above-mentioned sample groove is placed in pressure vessel, seal cover seal cover being locked with securing member then closes;
3, the pressure of the pressure-reducing valve assembly of pressure vessel is adjusted to 0.1Mpa, then the Compressed Gas output duct is inserted on the air inlet pipe of pressure-reducing valve, in pressure vessel, passes into Compressed Gas, when the pressure in pressure vessel reaches 0.1Mpa, automatically stop air inlet, and keep this pressure;
4, need open pressure vessel every day and measure the numerical value such as the proportion of acetone soln, fat content, and then by sealing for pressure vessels, and make the pressure in pressure vessel reach 0.1Mpa.When the numerical value such as the proportion of the acetone soln of measuring, fat content no longer change, the acetone that need to more renew, and then repeat above-mentioned measurement operation.To 1 week left and right time, the concentration of acetone no longer changed, and the fat content of acetone no longer increases, and the concentration of acetone is also in the normal concentration scope, and the now dehydration of sample, degreasing stage complete.
The present invention compared with prior art has following advantage:
1, greatly enhance productivity, foreshortened to the 1-2 time-of-week by original 4-6 month and can reach biological sample degreasing, dehydration indexes requirement.
2, save manpower, the tracing management time is very short.
3, reduce production costs, the number of times that acetone is changed is few, and volatile quantity is limited, saves raw material.
4, preparation of specimen's cycle short, can complete the making that some are badly in need of biological samples.
The accompanying drawing explanation
Fig. 1 is the three-dimensional simplified schematic diagram of initialization state of the present invention.
embodimentin the three-dimensional simplified schematic diagram of the initialization state of the present invention shown in Fig. 1, be provided with pressure-reducing valve assembly 2 and glass peephole 1 above the tank body 3 of horizontal pressure vessel, the tank body side opening is provided with the seal cover corresponding with it 5 and securing member.By in the sample groove of the biology of sample to be made interior Sheng acetone as shark is placed in 4 and the acetone liquid level need to cover sample fully, this sample groove is placed in pressure container tank body, seal cover seal cover being locked with securing member closes.Then the pressure of the pressure-reducing valve assembly of pressure vessel is adjusted to 0.1Mpa, again the Compressed Gas output duct is inserted on the air inlet pipe of pressure-reducing valve, in pressure vessel, passes into Compressed Gas, when the pressure in pressure vessel reaches 0.1Mpa, automatically stop air inlet, and keep this pressure.Need open pressure vessel every day and measure the numerical value such as the proportion of acetone soln, fat content, and then by sealing for pressure vessels, and make the pressure in pressure vessel reach 0.1Mpa.When the numerical value such as the proportion of the acetone soln of measuring, fat content no longer change, the acetone that need to more renew, and then measure the numerical value such as proportion, fat content of acetone soln every day.To the concentration of one week acetone no longer change, the fat content of acetone no longer increases, and the concentration of acetone is also in normal concentration scope 98%, the now dehydration of sample, the degreasing stage completes.
Claims (1)
1. one kind applies to plastify the pressure displacement method in sample dewatering and defatting process, it is characterized in that:
1) biology that will sample be made is placed in the sample groove of interior Sheng acetone;
2) above-mentioned sample groove is placed in pressure vessel, seal cover seal cover being locked with securing member then closes;
3) pressure of the pressure-reducing valve assembly of pressure vessel is adjusted to 0.1Mpa, then the Compressed Gas output duct is inserted on the air inlet pipe of pressure-reducing valve, in pressure vessel, passes into Compressed Gas, when the pressure in pressure vessel reaches 0.1Mpa, automatically stop air inlet, and keep this pressure;
4) need open pressure vessel every day and measure the numerical value such as the proportion of acetone soln, fat content, and then by sealing for pressure vessels, and make the pressure in pressure vessel reach 0.1Mpa, when the numerical value such as the proportion of the acetone soln of measuring, fat content no longer change, the acetone that need to more renew, and then repeat above-mentioned measurement operation, to 1 week left and right time, the concentration of acetone no longer changes, the fat content of acetone no longer increases, and the concentration of acetone is also in the normal concentration scope, the now dehydration of sample, the degreasing stage completes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103242968A CN103461319A (en) | 2013-07-30 | 2013-07-30 | Pressure displacement method applied in dehydration and degreasing of plasticized specimen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103242968A CN103461319A (en) | 2013-07-30 | 2013-07-30 | Pressure displacement method applied in dehydration and degreasing of plasticized specimen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103461319A true CN103461319A (en) | 2013-12-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013103242968A Pending CN103461319A (en) | 2013-07-30 | 2013-07-30 | Pressure displacement method applied in dehydration and degreasing of plasticized specimen |
Country Status (1)
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| CN (1) | CN103461319A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102125025A (en) * | 2010-12-17 | 2011-07-20 | 滨州医学院 | Method for plasticizing biological specimen |
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2013
- 2013-07-30 CN CN2013103242968A patent/CN103461319A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102125025A (en) * | 2010-12-17 | 2011-07-20 | 滨州医学院 | Method for plasticizing biological specimen |
Non-Patent Citations (2)
| Title |
|---|
| 侯皓天等: "低温真空脱水法在生物塑化标本中的应用", 《解剖学杂志》 * |
| 白剑等: "Hoffen P45生物塑化技术在断层标本制作中的应用", 《中国临床解剖学杂志》 * |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131225 |