CN103454361A - Method and device for extracting rhodamine (B) in food - Google Patents
Method and device for extracting rhodamine (B) in food Download PDFInfo
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- CN103454361A CN103454361A CN2013103323166A CN201310332316A CN103454361A CN 103454361 A CN103454361 A CN 103454361A CN 2013103323166 A CN2013103323166 A CN 2013103323166A CN 201310332316 A CN201310332316 A CN 201310332316A CN 103454361 A CN103454361 A CN 103454361A
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Abstract
The invention discloses a method and a device for extracting rhodamine (B) in food. The method comprises the following steps of (1) immersing food in clear water, standing and performing ultrasonic treatment to dissolve rhodamine (B) in the clear water, and then filtering to obtain immersion liquor; (2) performing liquid separation treatment on the immersion liquor to obtain supernatant liquor; and (3) adding normal hexane into the supernatant liquor, then uniformly mixing, and heating to volatilize the normal hexane to obtain rhodamine (B) in the food. The method disclosed by the invention is combined with an ultrasonic extraction way and a chemical extraction way; the extracted rhodamine (B) is high in purity, and the subsequent inspection work can be performed after the extracted rhodamine (B) is slightly processed.
Description
Technical field
The invention belongs to composition of food and extract field, be specifically related to extracting method and the equipment of rhodamine in a kind of food (B).
Background technology
The extraction of rhodamine in food (B) composition, be not only the primary link that food detects, and is also the prerequisite that spectrometer and mass spectrometer effectively detect, and therefore, effectively extraction and separate type are vital.In actual life, extraction and the separate mode of rhodamine (B) composition have many types, the many types such as chemical method, physical method.But directly the chemical method extraction efficiency is lower.How to adopt efficient, easy, to extract with purifying cheaply be very crucial.
Current rhodamine (B) composition extracting mode mainly adopts the ultrasound wave mode to extract, and the compounding substances for containing rhodamine (B) that this mode is extracted does not reach real material and extracts the purpose of purifying.According to actual requirement, if not only can extract, and can also purify, the sensitivity that guarantee detects like this.
Summary of the invention
The object of the invention is to overcome the prior art defect, the extracting method of rhodamine in a kind of food (B) is provided.
Another object of the present invention is to be provided for the equipment of said extracted method.
Technical scheme of the present invention is as follows:
The extracting method of rhodamine in a kind of food (B), comprise the steps:
(1) food is immersed in clear water, standing ultrasonic processing, be dissolved in clear water rhodamine (B) wherein, refilters to obtain soak solution;
(2) above-mentioned soak solution is carried out to the separatory processing, obtain supernatant;
(3) add normal hexane in above-mentioned supernatant, after mixing, heat again above-mentioned normal hexane is vapored away, obtain the rhodamine (B) in food.
In a preferred embodiment of the invention, the parameter of the ultrasonic processing of described step (1) is 40KHz, 60W.
In a preferred embodiment of the invention, also comprise the normal hexane of volatilization is taken away, in case contaminated air.
Another technical scheme of the present invention is as follows:
The equipment that in a kind of above-mentioned food, the extracting method of rhodamine (B) is used, comprise a sample ultrasonic processing apparatus and the solvent heated volatile device be located on same frame.
In a preferred embodiment of the invention, described sample ultrasonic treating apparatus comprises a ultrasonic pond, a top cover and a ultrasonic transducer, and ultrasonic transducer is connected to produce ultrasound wave in ultrasonic pond with ultrasonic pond, and the top cover lid is located at the upper shed in ultrasonic pond; Also be provided with an immersion bottle in this ultrasonic pond, in this immersion bottle, be provided with a Steel net, foodstuff samples is placed on and soaks in bottle, in preventing that swill from entering described soak solution when ultrasound wave is processed by Steel net.
In a preferred embodiment of the invention, described solvent heated volatile device comprises that one has body, a heating plate and a heat conducting sleeve of upper shed, heat conducting sleeve is located in body, heating plate is located at the bottom of body, installing liquid container in heat conducting sleeve, heat conducting sleeve is provided with temperature sensor outward, heating plate by the solution in the heat conducting sleeve liquid heating vessel so that solvent evaporates wherein.
In a preferred embodiment of the invention, also comprise a volatile gas, the upper shed of the described body gland bonnet that is pivoted, sealing covers and is provided with a snorkel to be communicated with liquid container and this volatile gas in heat conducting sleeve, after making the solvent evaporates of the solution in liquid container, by this volatile gas, siphoned away, with preventing air contamination.
In a preferred embodiment of the invention, the outer wall of described heat conducting sleeve is arranged with jacket.
In a preferred embodiment of the invention, an end of described gland bonnet is articulated in a side of the upper shed of body, and the other end of gland bonnet is connected with the opposite side of the upper shed of body by Spring lock catch.
In a preferred embodiment of the invention, also comprise a control module, this control module is electrically connected to temperature sensor and the heating plate of described sample ultrasonic treating apparatus and described solvent heated volatile device.
The invention has the beneficial effects as follows:
1, method of the present invention is extracted and the chemical extraction mode in conjunction with ultrasound wave, and the rhodamine extracted (B) purity is higher, processes and can carry out subsequent check work a little;
2, the extraction rate of method of the present invention is fast, has saved the time;
3, compact equipment of the present invention, overhaul conveniently;
4, equipment of the present invention is provided with volatile gas, is used for the solvent of volatilization is siphoned away, and has avoided air pollution;
5, equipment of the present invention is provided with a control module and a LED display panel, has realized control parameter visual, easy to operate of ultrasonic processing apparatus and solvent heated volatile device.
The accompanying drawing explanation
The schematic perspective view that Fig. 1 is equipment of the present invention;
The cut-open view of the ultrasonic processing apparatus that Fig. 2 is equipment of the present invention;
The cut-open view of the solvent heated volatile device that Fig. 3 is equipment of the present invention;
Embodiment
Below by reference to the accompanying drawings technical scheme of the present invention is further detailed and describes by embodiment.
As shown in Figure 1 to Figure 3, the equipment that in a kind of above-mentioned food, the extracting method of rhodamine (B) is used, comprise a control module 14, a sample ultrasonic processing apparatus 11, a solvent heated volatile device 12 and the volatile gas 13 be located on same frame, described control module 14 is electrically connected to above-mentioned ultrasonic sample processing device, solvent heated volatile device 12 and volatile gas 13.
Described sample ultrasonic treating apparatus 11 comprises a ultrasonic pond 111, a top cover 112 and a ultrasonic transducer 113, and ultrasonic transducer 113 is connected to produce ultrasound wave in ultrasonic pond 111 with ultrasonic pond 111, and top cover 112 lids are located at the upper shed in ultrasonic pond 111; Also be provided with an immersion bottle 114 in this ultrasonic pond 111, in this immersion bottle 114, be provided with a Steel net 115, foodstuff samples is placed on and soaks in bottle 114, in preventing that swill from entering described soak solution when ultrasound wave is processed by Steel net 115.
Described solvent heated volatile device 12 comprises that one has body 121, a heating plate 122 and a heat conducting sleeve 123 of upper shed, heat conducting sleeve 123 is located in body 121, heating plate 122 is located at the bottom of body 121, the interior installing liquid container 124 of heat conducting sleeve 123, the outer temperature sensor 129 that is provided with of heat conducting sleeve 123, the outer wall of heat conducting sleeve 123 is arranged with jacket 125, heating plate 122 by the solution in heat conducting sleeve 123 liquid heating vessels 124 so that solvent evaporates wherein.Preferably, the upper shed of described body 121 gland bonnet 126 that is pivoted, concrete, an end of sealing lid 126 is articulated in a side of the upper shed of body 121, and the other end of gland bonnet 126 is connected with the opposite side of the upper shed of body 121 by Spring lock catch 127.Sealing lid 126 is provided with a snorkel 128 to be communicated with liquid container 124 and this volatile gas 13 in heat conducting sleeve 123, by this volatile gas 13, is siphoned away after making the solvent evaporates of the solution in liquid container 124, with preventing air contamination.
Above-mentioned control module 14, comprise a temperature control device be electrically connected to the heating plate 122 of solvent heated volatile device 12 and temperature sensor 128 141, one group of control knob 142 and a LED display panel 143 be connected with said temperature control gear 141 of being electrically connected to volatile gas 13 with sample ultrasonic treating apparatus 11, this LED display panel 143 is in order to realize visual temperature control.
The operation parameter of sample ultrasonic treating apparatus 11 of the present invention is 40KHz, 60W, distinctive physical property when it utilizes sound wave to propagate in the liquid continuous medium: ultrasound wave produces the multistage effects such as mechanical vibration that distinctive " cavitation effect " and ultrasound wave introduce at Propagation, microjet, micro-acoustic streaming in liquid medium, all impel the effect of effective constituent to sovent diffusion, make the water-soluble solution of rhodamine (B) in food
Specifically, by peristaltic pump, in soaking bottle 114, add the 5mL clear water, foodstuff samples is positioned in screen pack, again screen pack is put into and soaked bottle 114, cover top cover 112, standing ultrasonic 2 minutes, rhodamine (B) wherein is dissolved in clear water, refilters to obtain soak solution;
(2) above-mentioned soak solution is carried out to the separatory processing by separating funnel, obtain supernatant;
(3) above-mentioned supernatant is poured in the liquid container 124 of solvent heated volatile device 12, then added normal hexane to mix;
(4) put into heat conducting sleeve 123 at aforesaid liquid container 124, cover gland bonnet 126, by control module 14, control heating-up temperature, the normal hexane to volatilize wherein starts volatile gas 13 to siphon away the normal hexane of volatilization simultaneously;
(5), after normal hexane has volatilized, obtain the rhodamine (B) in food;
(6) add 0.5mL clear water and a certain amount of Shinners in the rhodamine (B) of gained, stir, just configured rhodamine (B) system that Raman spectrometer detects of being convenient to.
The above, be only preferred embodiment of the present invention, therefore can not limit according to this scope of the invention process, the equivalence done according to the scope of the claims of the present invention and description changes and modifies, and all should still belong in the scope that the present invention contains.
Claims (10)
1. the extracting method of rhodamine (B) in a food, is characterized in that: comprise the steps:
(1) food is immersed in clear water, standing ultrasonic processing, be dissolved in clear water rhodamine (B) wherein, refilters to obtain soak solution;
(2) above-mentioned soak solution is carried out to the separatory processing, obtain supernatant;
(3) add normal hexane in above-mentioned supernatant, after mixing, heat again above-mentioned normal hexane is vapored away, obtain the rhodamine (B) in food.
2. the extracting method of rhodamine (B) in a kind of food as claimed in claim 1, it is characterized in that: the parameter of the ultrasonic processing of described step (1) is 40KHz, 60W.
3. the extracting method of rhodamine (B) in a kind of food as claimed in claim 1 or 2, is characterized in that: also comprise the normal hexane of volatilization is taken away, in case contaminated air.
4. the equipment that in a food claimed in claim 1, the extracting method of rhodamine (B) is used, is characterized in that: comprise a sample ultrasonic processing apparatus and the solvent heated volatile device be located on same frame.
5. equipment as claimed in claim 4, it is characterized in that: described sample ultrasonic treating apparatus comprises a ultrasonic pond, a top cover and a ultrasonic transducer, ultrasonic transducer is connected to produce ultrasound wave in ultrasonic pond with ultrasonic pond, and the top cover lid is located at the upper shed in ultrasonic pond; Also be provided with an immersion bottle in this ultrasonic pond, in this immersion bottle, be provided with a Steel net, foodstuff samples is placed on and soaks in bottle, in preventing that swill from entering described soak solution when ultrasound wave is processed by Steel net.
6. equipment as claimed in claim 4, it is characterized in that: described solvent heated volatile device comprises that one has body, a heating plate and a heat conducting sleeve of upper shed, heat conducting sleeve is located in body, heating plate is located at the bottom of body, installing liquid container in heat conducting sleeve, heat conducting sleeve is provided with temperature sensor outward, heating plate by the solution in the heat conducting sleeve liquid heating vessel so that solvent evaporates wherein.
7. equipment as claimed in claim 6, it is characterized in that: also comprise a volatile gas, the upper shed of the described body gland bonnet that is pivoted, sealing covers and is provided with a snorkel to be communicated with liquid container and this volatile gas in heat conducting sleeve, after making the solvent evaporates of the solution in liquid container, by this volatile gas, siphoned away, with preventing air contamination.
8. equipment as claimed in claim 7, it is characterized in that: the outer wall of described heat conducting sleeve is arranged with jacket.
9. equipment as claimed in claim 7, it is characterized in that: an end of described gland bonnet is articulated in a side of the upper shed of body, and the other end of gland bonnet is connected with the opposite side of the upper shed of body by Spring lock catch.
10. equipment as claimed in claim 7, it is characterized in that: also comprise a control module, this control module is electrically connected to temperature sensor and the heating plate of described sample ultrasonic treating apparatus and described solvent heated volatile device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103323166A CN103454361A (en) | 2013-08-01 | 2013-08-01 | Method and device for extracting rhodamine (B) in food |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103323166A CN103454361A (en) | 2013-08-01 | 2013-08-01 | Method and device for extracting rhodamine (B) in food |
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| Publication Number | Publication Date |
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| CN103454361A true CN103454361A (en) | 2013-12-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2013103323166A Pending CN103454361A (en) | 2013-08-01 | 2013-08-01 | Method and device for extracting rhodamine (B) in food |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683612A (en) * | 2020-12-01 | 2021-04-20 | 颜妮 | Detection device and detection method for pesticide residue content of fruits |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2808258Y (en) * | 2005-06-20 | 2006-08-23 | 宁波振国制药设备制造有限公司 | Multifunctional extraction device for laboratory |
| CN200966767Y (en) * | 2006-11-10 | 2007-10-31 | 清华大学 | Integrated device for distilling the effective component from the Chinese traditional medicine |
| CN101239249A (en) * | 2008-03-06 | 2008-08-13 | 永定采善堂生物质提炼有限公司 | Alcohol-soluble plant component extraction device |
| CN202478645U (en) * | 2012-01-10 | 2012-10-10 | 成都科尔医药技术有限公司 | Ultrasonic extraction and concentration circulation device |
| CN203551529U (en) * | 2013-08-01 | 2014-04-16 | 厦门大学 | Extraction equipment for Rhodamine B in foods |
-
2013
- 2013-08-01 CN CN2013103323166A patent/CN103454361A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2808258Y (en) * | 2005-06-20 | 2006-08-23 | 宁波振国制药设备制造有限公司 | Multifunctional extraction device for laboratory |
| CN200966767Y (en) * | 2006-11-10 | 2007-10-31 | 清华大学 | Integrated device for distilling the effective component from the Chinese traditional medicine |
| CN101239249A (en) * | 2008-03-06 | 2008-08-13 | 永定采善堂生物质提炼有限公司 | Alcohol-soluble plant component extraction device |
| CN202478645U (en) * | 2012-01-10 | 2012-10-10 | 成都科尔医药技术有限公司 | Ultrasonic extraction and concentration circulation device |
| CN203551529U (en) * | 2013-08-01 | 2014-04-16 | 厦门大学 | Extraction equipment for Rhodamine B in foods |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112683612A (en) * | 2020-12-01 | 2021-04-20 | 颜妮 | Detection device and detection method for pesticide residue content of fruits |
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Application publication date: 20131218 |
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