CN103451240B - A kind of plant material that adds is to improve the method for production of butanol bacterium leavening property - Google Patents
A kind of plant material that adds is to improve the method for production of butanol bacterium leavening property Download PDFInfo
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- CN103451240B CN103451240B CN201210171575.0A CN201210171575A CN103451240B CN 103451240 B CN103451240 B CN 103451240B CN 201210171575 A CN201210171575 A CN 201210171575A CN 103451240 B CN103451240 B CN 103451240B
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Abstract
The invention discloses a kind of plant material that adds to improve the method for production of butanol bacterium leavening property.Namely in fermention medium, a small amount of plant material is added at production of butanol bacterium fermentation initial period, to shorten production of butanol bacterium fermentation lag phase; Produce acid phase production of butanol bacterium and in fermention medium, add a small amount of plant material, to improve the solvent conversion rate of production of butanol bacterium.The plant material that the present invention adopts comprises maize straw, buckwheat bar, rice straw, wheat-straw, Pericarppium arachidis hypogaeae, Pericarpium Mali pumilae, dried orange peel, the root of kudzu vine, soybean, Ginkgo Leaf, the leaf of bamboo and Semen Vitis viniferae.Adopt method of the present invention, not activated production of butanol bacterium can be directly used in fermentative production, and its fermentation lag phase foreshortens to 6-12h by 18-24h, and in fermented liquid, by product butyric acid and acetic acid concentration are reduced to 1-2g/L by 3-5g/L.The problem that the bacterial classification that the invention solves the common existence of production of butanol bacterium is easily degenerated, the lag phase that ferments is long, improves the solvent production of production of butanol bacterium.
Description
Technical field
Present method belongs to fermentation industry field, and particularly a kind of plant material that adds is to improve the method for production of butanol bacterium leavening property.
Background technology
Butanols is important large industrial chemicals, it is again a kind of liquid fuel of new generation having development prospect after ethanol, by producing the anaerobically fermenting of solvent clostridium Clostridia, can by suitable carbohydrate-modifying be acetone (Acetone), butanols (Butanol) and ethanol (Ethanol) equal solvent, thus this kind solvent production technology is also called ABE fermentation for short.
The research of acetone butanol fermentation started from for 19th century.Arrive in 20 beginnings of the century, due to the development of automotive industry, need a large amount of acetone and butanols as elastomeric raw material, this facilitates acetone butanol fermentation industrial expansion to a certain extent.By 1914, famous doctor ChaimWeizmann successfully isolated a strain bacterium, called after Clostridiumacetobutylicum(clostridium acetobutylicum), it can ferment various cereal materials generate solvent.This bacterium be found to be acetone, the industrialization of butylic fermentation lays a good foundation.In the World War I of outburst in 1914, acetone is as the raw material manufacturing explosive, and its demand increases greatly.Britain, the U.S., the states such as Japan establish Duo Jia acetone butanol fermentation factory, and this makes the industrial second largest fermentation industry become after ethanol fermentation industry of acetone, butylic fermentation.Because clostridium acetobutylicum is strictly anaerobic bacterium, this industry also becomes anaerobically fermenting industry maximum in history.
Clostridium Clostridia is the exclusive source producing solvent industry bacterial classification at present.In recent years, by the comparative studies of systematics, genomic dna/DNA hybridization and the aspect such as DNA fingerprinting and leavening property with think, industrial product solvent clostridium is classified as 4 " kind " (Species), all original amylofermentation type bacterial strains belong to an independent kind, i.e. clostridium acetobutylicum Clostridiumacetobutylicum.Such bacterium presents stronger amylase activity, be applicable to the starchy material such as fermented maize and cereal, there is unique phylogeny characteristic simultaneously, with other 3 kinds be closely related, namely the sibship of Bai Shi clostridium Clostridiumbeijerinckii, clostridium saccharobutyricum ClostridiumSaccharobutylicum and clostridium saccharoacetoperbutylicum Clostridiumsaccharoperbutylacetonicum is far away.
Clostridium acetobutylicum (C.acetobutylicum) produces spore anerobe as a class, and its fermenting process is divided into the product acid phase and produces two stages of solvent phase.But find after carrying out continuous print Secondary Culture to this bacterium, it produces solvent power and can degenerate gradually, this is in product solvent fermentation process, especially in Continuous Fermentation Processes, is a comparatively serious problem.Have researcher to find, bacterial classification goes down to posterity more than 10 times, just loses the ability of glucose fermentation.In order to shorten the lag phase of fermenting process, increase bacterial classification and produce solvent power, usual clostridium acetobutylicum need be stored in enriched medium base, have to pass through before fermentation activation culture and heat shock (heat-shocking) change could in starch or dextrose culture-medium well-grown.
Along with generating a large amount of H in clostridium acetobutylicum metabolic process
2, this reaction mainly relies on the catalysis of ferredoxin oxide-reductase and hydrogen enzyme to complete.Research proves, closely related with redox potential in substratum in clostridium acetobutylicum process of growth, NADH
2/ NAD, as the electron donor of born of the same parents' internal oxidition reduction reaction, increases NADH
2the transformation efficiency of/NAD can increase the output of butanols.In order to maintain NADH best in microorganism cells at micro-oxygen or anaerobic condition
2/ NAD ratio, can adopt and add electron acceptor(EA) or change substrate oxidation reduced state to realize NADH
2the adjustment of/NAD ratio.There are some researches show, aldehyde, ketone, acid, dinitrogen or nitrate all as exogenous electron acceptor, can accelerate NADH oxidation, maintain cell and are in optimum oxidation reduced state.But the small organic molecule such as different sorts aldehyde, ketone, acid is (as syringic aldehyde, forulic acid, Radix Puerariae isoflavone) impact of growth be there are differences, and in fermention medium, directly add the organism such as aldehyde, ketone, acid, when these exogenous electron acceptor densities are higher than 10mg/L-100mg/L, restraining effect will be caused to thalli growth.
Plant material chemical constitution is complicated, and its water-soluble substances comprises the organism such as multiple aldehyde, ketone, acid.Such as, in plant material xylogen compositing monomer forulic acid, the materials such as flavones just show oxidation-resistance in biological metabolism, have the ability of receiving and losing electrons.Infer thus and add a small amount of plant material in fermention medium, will contribute to regulating spawn culture condition, and accelerate NADH accretion rate in fermentation approach, thus improve the leavening property of clostridium acetobutylicum.
The invention provides a kind of plant material that adds to improve the method for production of butanol bacterium leavening property, namely in fermention medium, a small amount of plant material is added at production of butanol bacterium fermentation initial period and product acid phase respectively, not activated production of butanol bacterium can be made to be directly used in fermentative production, shorten production of butanol bacterium fermentation lag phase, improve the solvent conversion rate of production of butanol bacterium simultaneously.The problem that the preservation bacterial classification that the invention solves the common existence of production of butanol bacterium is easily degenerated, the lag phase that ferments is long, improves the solvent production of production of butanol bacterium simultaneously, reduces the ratio of by product butyric acid and acetic acid in fermented liquid.
Summary of the invention
The object of the present invention is to provide a kind of plant material that adds to improve the method for production of butanol bacterium leavening property, namely by adding a small amount of plant material at production of butanol bacterium fermentation initial period in fermention medium, to shorten production of butanol bacterium fermentation lag phase; Produce acid phase production of butanol bacterium and add a small amount of plant material in fermention medium, to improve the solvent conversion rate of production of butanol bacterium, reduce the ratio of by product butyric acid and acetic acid in fermented liquid.
Technical scheme of the present invention is as follows:
A kind of plant material that adds provided by the invention is to improve the method for production of butanol bacterium leavening property, and its step is as follows:
1) preparation of plant material:
The present invention's plant material used comprises maize straw, buckwheat stalk, rice straw, wheat-straw, Pericarppium arachidis hypogaeae, Pericarpium Mali pumilae, dried orange peel, the root of kudzu vine, soybean, Ginkgo Leaf, one of in the leaf of bamboo and Semen Vitis viniferae or arbitrary combination.
Plant material tap water is cleaned up, naturally dries.Insert in beaker after being pulverized by plant material with coffee bean grinder (TSK-927S, Cankun Industrial Co., Ltd., Xiamen City), at 115 DEG C, sterilizing 15min is stand-by.
2) bacterial classification and culture condition:
One of the production of butanol bacterium that the present invention adopts is clostridium acetobutylicum (Clostridiumacetobutylicum), in Bai Shi clostridium (Clostriridiumbeijerinckii) and clostridium saccharobutyricum (Clostridiumsaccharobutylicum).
Production of butanol bacterium be deposited in the corn mash substratum of 6%, bacterial classification at 4 DEG C preservation 2-3 month.
The fermention medium of production of butanol bacterium often rises containing glucose 60g, ammonium acetate 4.3g, potassium primary phosphate 1.768g, dipotassium hydrogen phosphate 2.938g, Para-Aminobenzoic 10mg, vitamin H 10mg, inorganic salt solution 1mL, pH6.5-7.0.Wherein 1L inorganic salt solution consists of: NaMoO
42H
2o2.4g; CoCl
26H
2o0.24g; CaCl
22H
2o, 1.5g; FeCl
36H
2o27g; H
2sO
428ml; CuSO
45H
2o0.25g; ZnSO
47H
2o0.29g; MnSO
4h
2o1.7g; MgSO
412g.
Fermention medium is sterilizing 15min at 115 DEG C, is cooled to room temperature, and the preservation of bacteria strain of the production of butanol bacterium of access 5 ~ 10%, static Anaerobic culturel 96h at 35 DEG C-37 DEG C, with the product assay in gas Chromatographic Determination fermented liquid.
3) in substratum, plant material is added in fermenting process:
When production of butanol bacterium fermentation 0-10h, add the plant material of 0.1-10g/L pulverizing in fermention medium after, when continuing Anaerobic culturel 24-48h, in substratum, still add the plant material that 0.1-10g/L pulverizes, Anaerobic culturel is until fermentation stops.
Embodiment
The invention is further illustrated by the following examples.
Embodiment 1
Semen Vitis viniferae tap water is cleaned up, naturally dries.Insert in beaker after being pulverized by Semen Vitis viniferae with coffee bean grinder, at 115 DEG C, sterilizing 15min is stand-by.
Clostridium acetobutylicum (ClostridiumacetobutylicumATCC824) is deposited in the corn mash substratum of 6%, and preserves 2 months at 4 DEG C.In fermention medium (formula is shown in summary of the invention), add 0.1g/L, 2g/L and 10g/L Semen Vitis viniferae be respectively prepared into No. 1, substratum, No. 2, substratum and No. 3, substratum.By 3 kinds of substratum sterilizing 15min at 115 DEG C, after being cooled to room temperature, clostridium acetobutylicum (Clostridiumacetobutylicum) preservation of bacteria strain of access 5%.Bacterial classification is static Anaerobic culturel at 37 DEG C.
For No. 1, substratum, when after strain fermentation 10h, in substratum, add 2g/L Semen Vitis viniferae; When after strain fermentation 24h, in substratum, add 8g/L Semen Vitis viniferae, afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 2, substratum, when after strain fermentation 48h, in substratum, add 8g/L Semen Vitis viniferae, afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 3, substratum, when after strain fermentation 48h, bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
Not add the fermention medium substratum in contrast of Semen Vitis viniferae.Fermention medium (formula is shown in summary of the invention) sterilizing 15min at 115 DEG C, after being cooled to room temperature, directly accesses clostridium acetobutylicum (Clostridiumacetobutylicum) preservation of bacteria strain of 5%.Bacterial classification is static Anaerobic culturel at 35 DEG C.
Gas chromatography determination is adopted to add each tunning content (table 1) in 3 kinds of substratum of Semen Vitis viniferae and control medium.
The promoter action of Semen Vitis viniferae to production of butanol bacterium leavening property added by table 1
Embodiment 2
By maize straw, buckwheat stalk, rice straw and wheat-straw tap water clean up, and naturally dry.With coffee bean grinder by after Four Plants raw material pulverizing, insert in different beakers respectively, at 115 DEG C, sterilizing 15min is stand-by.
Visit formula clostridium (ClostridiumbeijerinckiiAS1.2127) to be deposited in the corn mash substratum of 6%, and preserve 2 months at 4 DEG C.In fermention medium (formula is shown in summary of the invention, and wherein glucose concn is 40g/L), add 0.1g/L, 1g/L and 2g/L buckwheat stalk be respectively prepared into No. 1, substratum, No. 2, substratum and No. 3, substratum.By 3 kinds of substratum sterilizing 15min at 115 DEG C, after being cooled to room temperature, formula of visiing clostridium (ClostridiumbeijerinckiiAS1.2127) preservation of bacteria strain of access 10%.Bacterial classification is static Anaerobic culturel at 37 DEG C.
For No. 1, substratum, when after strain fermentation 10h, in substratum, add 2g/L maize straw; When after strain fermentation 24h, in substratum, add 2g/L maize straw, afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 2, substratum, when after strain fermentation 48h, in substratum, add 4g/L rice straw, afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 3, substratum, when after strain fermentation 48h, in substratum, add 4g/L wheat-straw, bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
Not add maize straw, buckwheat stalk, the fermention medium substratum in contrast of rice straw and wheat-straw.Fermention medium (formula is shown in summary of the invention, and wherein glucose concn is 40g/L) sterilizing 15min at 115 DEG C, after being cooled to room temperature, formula of visiing clostridium (Clostridiumbeijerinckii) preservation of bacteria strain of directly access 10%.Bacterial classification is static Anaerobic culturel at 35 DEG C.
Gas chromatography determination is adopted to add each tunning content (table 2) in 3 kinds of substratum of plant material and control medium.
Maize straw added by table 2, buckwheat stalk, and rice straw and wheat-straw are to the promoter action of production of butanol bacterium leavening property
Embodiment 3
Pericarppium arachidis hypogaeae tap water is cleaned up, naturally dries.With coffee bean grinder Pericarppium arachidis hypogaeae pulverized rearmounted enter in beaker, at 115 DEG C, sterilizing 15min is stand-by.
Clostridium acetobutylicum (ClostridiumacetobutylicumATCC824) is deposited in the corn mash substratum of 6%, and preserves 2 months at 4 DEG C.In fermention medium (formula is shown in summary of the invention), add 0.1g/L, 2g/L and 10g/L Pericarppium arachidis hypogaeae be respectively prepared into No. 1, substratum, No. 2, substratum and No. 3, substratum.By 3 kinds of substratum sterilizing 15min at 115 DEG C, after being cooled to room temperature, clostridium acetobutylicum (Clostridiumacetobutylicum) preservation of bacteria strain of access 5%.Bacterial classification is static Anaerobic culturel at 37 DEG C.
Not add the fermention medium substratum in contrast of Pericarppium arachidis hypogaeae.Fermention medium (formula is shown in summary of the invention) sterilizing 15min at 115 DEG C, after being cooled to room temperature, directly accesses clostridium acetobutylicum (Clostridiumacetobutylicum) preservation of bacteria strain of 5%.Bacterial classification is static Anaerobic culturel at 35 DEG C.
Gas chromatography determination is adopted to add each tunning content (table 3) in 3 kinds of substratum of Pericarppium arachidis hypogaeae and control medium.
The promoter action of Pericarppium arachidis hypogaeae to production of butanol bacterium leavening property added by table 3
Embodiment 4
Pericarpium Mali pumilae and dried orange peel tap water are cleaned up, naturally dries.To insert in beaker after two kinds of raw material pulverizing with coffee bean grinder, at 115 DEG C, sterilizing 15min be stand-by.
Visit formula clostridium (ClostridiumbeijerinckiiAS1.2127) to be deposited in the corn mash substratum of 6%, and preserve 2 months at 4 DEG C.In fermention medium (formula is shown in summary of the invention, glucose concn 40g/L), add 2g/L, 5g/L, 10g/L Pericarpium Mali pumilae be respectively prepared into No. 1, substratum, No. 2, substratum and No. 3, substratum.By 3 kinds of substratum sterilizing 15min at 115 DEG C, after being cooled to room temperature, formula of visiing clostridium (Clostridiumbeijerinckii) preservation of bacteria strain of access 10%.Bacterial classification is static Anaerobic culturel at 37 DEG C.
For No. 1, substratum, when after strain fermentation 10h, in substratum, add 2g/L dried orange peel; Afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 2, substratum, when after strain fermentation 48h, in substratum, add 2g/L dried orange peel, afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 3, substratum, when after strain fermentation 48h, bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
Not add the fermention medium substratum in contrast of Pericarpium Mali pumilae and dried orange peel.Fermention medium (formula is shown in summary of the invention, glucose concn 40g/L) sterilizing 15min at 115 DEG C, after being cooled to room temperature, formula of visiing clostridium (Clostridiumbeijerinckii) preservation of bacteria strain of directly access 10%.Bacterial classification is static Anaerobic culturel at 35 DEG C.
Gas chromatography determination is adopted to add each tunning content (table 4) in 3 kinds of substratum of Pericarpium Mali pumilae and dried orange peel and control medium.
Table 4 adds Pericarpium Mali pumilae and dried orange peel to the promoter action of the leavening property of clostridium acetobutylicum
Embodiment 5
Root of kudzu vine tap water is cleaned up, naturally dries.To insert in beaker after corn straw smashing with coffee bean grinder, at 115 DEG C, sterilizing 15min be stand-by.
Clostridium saccharoacetoperbutylicum (Clostridiumsaccharoperbutylacetonicum) is deposited in the corn mash substratum of 6%, and preserves 2 months at 4 DEG C.In fermention medium (formula is shown in summary of the invention, glucose concn 40g/L), add 0.1g/L, 0.5g/L and the 1g/L root of kudzu vine be respectively prepared into No. 1, substratum, No. 2, substratum and No. 3, substratum.By 3 kinds of substratum sterilizing 15min at 115 DEG C, after being cooled to room temperature, clostridium saccharoacetoperbutylicum (Clostridiumsaccharoperbutylacetonicum) preservation of bacteria strain of access 10%.Bacterial classification is static Anaerobic culturel at 37 DEG C.
For No. 1, substratum, when after strain fermentation 10h, in substratum, add the 2g/L root of kudzu vine; When after strain fermentation 24h, in substratum, add the 2g/L root of kudzu vine, afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 2, substratum, when after strain fermentation 48h, in substratum, add the 4g/L root of kudzu vine, afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 3, substratum, when after strain fermentation 48h, bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
Not add the fermention medium substratum in contrast of the root of kudzu vine.(formula is shown in summary of the invention to fermention medium, glucose concn 40g/L) sterilizing 15min at 115 DEG C, after being cooled to room temperature, directly access clostridium saccharoacetoperbutylicum (Clostridiumsaccharoperbutylacetonicum) preservation of bacteria strain of 10%.Bacterial classification is static Anaerobic culturel at 35 DEG C.
Gas chromatography determination is adopted to add each tunning content (table 5) in 3 kinds of substratum of the root of kudzu vine and control medium.
The promoter action of the root of kudzu vine to production of butanol bacterium leavening property added by table 5
Embodiment 6
Soybean tap water is cleaned up, naturally dries.With coffee bean grinder soybean pulverized rearmounted enter in beaker, at 115 DEG C, sterilizing 15min is stand-by.
Clostridium saccharoacetoperbutylicum (Clostridiumsaccharoperbutylacetonicum) is deposited in the corn mash substratum of 6%, and preserves 2 months at 4 DEG C.In fermention medium (formula is shown in summary of the invention, glucose concn 40g/L), add 0.5g/L, 2g/L and 5g/L soybean be respectively prepared into No. 1, substratum, No. 2, substratum and No. 3, substratum.By 3 kinds of substratum sterilizing 15min at 115 DEG C, after being cooled to room temperature, clostridium saccharoacetoperbutylicum (Clostridiumsaccharoperbutylacetonicum) preservation of bacteria strain of access 10%.Bacterial classification is static Anaerobic culturel at 37 DEG C.
For No. 1, substratum, when after strain fermentation 10h, in substratum, add 2g/L soybean; When after strain fermentation 24h, in substratum, add 2.5g/L soybean, afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 2, substratum, when after strain fermentation 48h, in substratum, add 3g/L soybean, afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 3, substratum, when after strain fermentation 48h, bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
Not add the fermention medium substratum in contrast of soybean.(formula is shown in summary of the invention to fermention medium, glucose concn 40g/L) sterilizing 15min at 115 DEG C, after being cooled to room temperature, directly access clostridium saccharoacetoperbutylicum (Clostridiumsaccharoperbutylacetonicum) preservation of bacteria strain of 10%.Bacterial classification is static Anaerobic culturel at 35 DEG C.
Gas chromatography determination is adopted to add each tunning content (table 6) in 3 kinds of substratum of soybean and control medium.
The promoter action of soybean to production of butanol bacterium leavening property added by table 6
Embodiment 7
Ginkgo Leaf and leaf of bamboo tap water are cleaned up, naturally dries.To insert in beaker after two kinds of raw material pulverizing with coffee bean grinder, at 115 DEG C, sterilizing 15min be stand-by.
Visit formula clostridium (ClostridiumbeijerinckiiAS1.2127) to be deposited in the corn mash substratum of 6%, and preserve 2 months at 4 DEG C.In fermention medium (formula is shown in summary of the invention, glucose concn 40g/L), add 0.2g/L, 0.5g/L, 1g/L Ginkgo Leaf be respectively prepared into No. 1, substratum, No. 2, substratum and No. 3, substratum.By 3 kinds of substratum sterilizing 15min at 115 DEG C, after being cooled to room temperature, formula of visiing clostridium (Clostridiumbeijerinckii) preservation of bacteria strain of access 10%.Bacterial classification is static Anaerobic culturel at 37 DEG C.
For No. 1, substratum, when after strain fermentation 10h, in substratum, add the 2g/L leaf of bamboo; Afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 2, substratum, when after strain fermentation 48h, in substratum, add the 2g/L leaf of bamboo, afterwards bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
For No. 3, substratum, when after strain fermentation 48h, bacterial classification at 35 DEG C static Anaerobic culturel until fermentation ends.
Not add the fermention medium substratum in contrast of Ginkgo Leaf and the leaf of bamboo.Fermention medium (formula is shown in summary of the invention, glucose concn 40g/L) sterilizing 15min at 115 DEG C, after being cooled to room temperature, formula of visiing clostridium (Clostridiumbeijerinckii) preservation of bacteria strain of directly access 10%.Bacterial classification is static Anaerobic culturel at 35 DEG C.
Gas chromatography determination is adopted to add each tunning content (table 7) in 3 kinds of substratum of Ginkgo Leaf and the leaf of bamboo and control medium.
Table 7 adds Ginkgo Leaf and the leaf of bamboo to the promoter action of production of butanol bacterium leavening property
Claims (4)
1. add plant material to improve a method for production of butanol bacterium leavening property, it is characterized in that following steps:
1) by the fermention medium of not activated production of butanol bacterium access sterilizing, Anaerobic culturel at 35 DEG C-38 DEG C;
2) when production of butanol bacterium is in fermentation initial period, add the plant material of 0.1-10g/L pulverizing in fermention medium after, Anaerobic culturel at 35 DEG C-38 DEG C is continued; Described plant material is maize straw, buckwheat stalk, rice straw, wheat-straw, Pericarppium arachidis hypogaeae, Pericarpium Mali pumilae, dried orange peel, the root of kudzu vine, soybean, Ginkgo Leaf, one of in the leaf of bamboo and Semen Vitis viniferae or arbitrary combination;
3) when production of butanol bacterium is in product acid phase, in substratum, still add the plant material that 0.1-10g/L pulverizes, Anaerobic culturel at 35 DEG C-38 DEG C is until fermentation stops; Described plant material is maize straw, buckwheat stalk, rice straw, wheat-straw, Pericarppium arachidis hypogaeae, Pericarpium Mali pumilae, dried orange peel, the root of kudzu vine, soybean, Ginkgo Leaf, one of in the leaf of bamboo and Semen Vitis viniferae or arbitrary combination.
2. method according to claim 1, it is characterized in that described production of butanol bacterium is clostridium acetobutylicum (Clostridiumacetobutylicum), Bai Shi clostridium (Clostriridiumbeijerinckii), clostridium saccharoacetoperbutylicum (Clostridiumsaccharoperbutylacetonicum) or clostridium saccharobutyricum (Clostridiumsaccharobutylicum).
3. method according to claim 1, is characterized in that described fermentation initial period is for fermentation 0-10h.
4. method according to claim 1, is characterized in that described product acid phase is for fermentation 24-48h.
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| 影响丙酮丁醇发酵的主要因素及解决方案的研究进展;高凯等;《生物质化学工程》;20110331;第45卷(第2期);45-50 * |
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