CN103451120A - Enrichment culture method of ammonia-oxidizing archaea in sewage treatment system - Google Patents
Enrichment culture method of ammonia-oxidizing archaea in sewage treatment system Download PDFInfo
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- CN103451120A CN103451120A CN2013100416332A CN201310041633A CN103451120A CN 103451120 A CN103451120 A CN 103451120A CN 2013100416332 A CN2013100416332 A CN 2013100416332A CN 201310041633 A CN201310041633 A CN 201310041633A CN 103451120 A CN103451120 A CN 103451120A
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Abstract
The invention provides an enrichment culture method of ammonia-oxidizing archaea in a sewage treatment system. The enrichment culture method comprises the following steps: A, preparing an enrichment culture medium of an ammonia-oxidizing bacteria flora; B, carrying out enrichment culture onto an ammonia-oxidizing bacteria flora, wherein a biological membrane or active sludge containing the ammonia-oxidizing archaea is inoculated in the enrichment culture medium; C, preparing an enrichment culture medium of the ammonia-oxidizing archaea, wherein antibiotics are added into the enrichment culture medium of the ammonia-oxidizing bacteria flora; and D, carrying out enrichment culture on the ammonia-oxidizing archaea, wherein a filter is used for filtering an enrichment culture solution and then transferring into the enrichment culture medium of the ammonia-oxidizing archaea in the step C to obtain the enrichment culture solution of the ammonia-oxidizing archaea with a higher purity. Possibility is provided for researching physiological and biochemical characteristics of the ammonia-oxidizing archaea by the enrichment culture solution of the ammonia-oxidizing archaea with the higher purity; the ammonia-oxidizing archaea has stronger tolerance; if the ammonia-oxidizing archaea is introduced to an ammonia-oxidizing enhancing technology, important practical significance is provided for improving biological denitrification capacity of the sewage treatment.
Description
Technical field
The present invention relates to the environmental microorganism field, specifically a kind of enrichment culture method of processing the ammonia oxidation archeobacteria of ammonia nitrogen in waste water.
Background technology
Bio-denitrification technology is one of technology the most important, the most commonly used in water environment protection, and the ammonia oxidation step in nitrifying process is committed step and the rate-limiting step of bio-denitrification technology.Research for a long time thinks that the major microorganisms that participates in this process is ammonia oxidizing bacteria always, but research in recent years finds that a kind of ancient bacterium also has the ammonia oxidation function, is known as the ammonia oxidation archeobacteria.Although in Sewage treatment systems, ammonia oxidizing bacteria is being taken on important role in the ammonia oxidation process, and it carries out biological denitrificaion also certain defect.At first be ammonia oxidizing bacteria quantity seldom, usually only account for 0.1% of bacteria total amount, in urban sewage system especially lower than 0.01%; Next is poor growth, and generation time is longer, and laboratory culture, domestication difficulty are higher: having is exactly that affected by ecological factor larger again, is difficult to grow under the environment such as high temperature, low dissolved axygen.
And, for the ammonia oxidation archeobacteria, research shows, in ecotope, the ammonia oxidation archeobacteria is widely distributed, quantity is huge, and part ammonia oxidation archeobacteria can extensively survive in low dissolved oxygen, high temperature, have a liking in the extreme environment such as acid/alkali, and this may for carrying out under extreme environmental conditions that sewage disposal provides.
Prior art is described: the method for the traditional enrichments such as doubling dilution plate streaking screening bacterium is to ammonia oxidation archeobacteria inapplicable.Traditional enriching method to the ammonia oxidation archeobacteria in Sewage treatment systems just adopts sanitary sewage or artificial distribution to carry out in experimental installation, after enrichment reaches detection line, is mainly used to analyze the population structure of ammonia oxidation archeobacteria.
In traditional method of utilizing sanitary sewage or artificial distribution's enrichment culture ammonia oxidation archeobacteria, have a large amount of heterotrophic bacteriums in the enrichment culture system, the proportion of ammonia oxidation archeobacteria in system is very little, is difficult to obtain the higher ammonia oxidation archeobacteria enrichment culture liquid of purity.
Summary of the invention
Technical problem to be solved by this invention is to provide the ammonia oxidation archeobacteria enrichment culture liquid that a kind of purity is higher.A kind of method of ammonia oxidation archeobacteria enrichment culture in Sewage treatment systems comprises following step:
A, preparation ammonia oxidation flora enrichment medium;
The enrichment culture of B, ammonia oxidation flora: will contain ammonia oxidation archeobacteria microbial film or active sludge and be inoculated in enrichment medium;
The enrichment medium of C, preparation ammonia oxidation archeobacteria: in the enrichment medium of ammonia oxidation flora, add microbiotic;
The enrichment culture method of D, ammonia oxidation archeobacteria: after with strainer, enrichment culture liquid being filtered, filtrate is transferred in the ammonia oxidation archeobacteria enrichment medium in step C, obtains the enrichment culture liquid of the ammonia oxidation archeobacteria that purity is higher.
The present invention adopts above technical scheme, its advantage is, strainer filters enrichment culture liquid and add usually enrichment culture ammonia oxidation of antibiosis archeobacteria in substratum, can remove a large amount of miscellaneous bacteria in ammonia oxidation archeobacteria enrichment culture liquid, obtains the higher ammonia oxidation archeobacteria enrichment culture liquid of purity.
Preferably, in described steps A, enrichment medium comprises: NaCl, MgCl
26H
2o, KCl, CaCl
2, KBr, KH
2pO
4.
Preferably, in described steps A, NaCl, MgCl
26H
2o, KCl, CaCl
2, KBr and KH
2pO
4mass ratio be 10:4:5:1:1:2.
Preferably, in described steps A, the compound method of enrichment medium comprises: the solution in enrichment medium, at 121 ℃ of lower sterilizing 30min, is then added with the NH after 0.2 μ m membrane filtration
4cl solution, NaHCO
3solution, EDTA Na-Fe solution, Sodium.alpha.-ketopropionate solution, trace element, VITAMIN and selenium tungsten solution.
Preferably, in described steps A, micro-compound method: 1.5g FeCl
24H
2o first is dissolved in 10mL HCl, then adds CoCl
26H
2o:190mg, MnCl
26H
2o:100mg, ZnCl
2: 70mg, HBO
3: 62mg, Na
2moO
42H
2o:36mg, NiCl
26H
2o:24mg, CuCl
22H
2o:17mg, add water and be settled to 1000mL.
Preferably, in described steps A, vitamin formula is: PABA: 40mg, and Bio: 10mg, nicotinic acid: 100mg, calcium D-VB5 calcium: 50mg, pyridoxine hydrochloride: 150mg, water 1000mL, 10mM buffer solution of sodium phosphate adjust pH is 7.1.
Preferably, in described steps A, selenium tungsten solution formula comprises: NaOH:0.4g, Na
2seO
35H
2o:6mg, Na
2wO
42H
2o:8mg, water 1000mL.
Preferably, in described step B, the pH value is 7.2-7.5, standing cultivation in the incubator that is 37 ± 1 ℃ in temperature.
Preferably, in described step C, the interpolation concentration of penicillin, Streptomycin sulphate and penbritin is 50mg/L.
The present invention further adopts above technical scheme, and its advantage is, added three kinds of antibiotic concentration are all 50mg/L, can suppress the growth of some bacteriums, but the growth of ammonia oxidation archeobacteria is not had to restraining effect.
Preferably, in described step D, strainer adopts 0.45 μ m film syringe-driven filter.
Preferably, NH in described enrichment culture liquid
4 +the concentration of-N remains at below 1mM.
Preferably, in described step D, regulating the pH value is 7.2-7.5, standing cultivation in the incubator that is 37 ± 1 ℃ in temperature.
Preferably, in described step B, incubation time be take 14-16 days as generation culture cycle, cultivates 2 cycles in dark environment, and supplements NH every 2-3 days
4cl and NaHCO
3.
Preferably, in described step D, in dark environment, cultivate 3 cycles, and supplemented NH every 3-5 days
4cl and NaHCO
3.Can obtain the ammonia oxidation archeobacteria enrichment culture liquid that purity is higher.
The invention has the beneficial effects as follows: the research that the ammonia oxidation enrichment culture liquid that purity is higher is ammonia oxidation archeobacteria physio-biochemical characteristics provides may, the ammonia oxidation archeobacteria has stronger patience, if the ammonia oxidation archeobacteria is incorporated among the ammonia oxidation intensifying technology, will have important practical significance to improving sewage disposal biological denitrificaion ability.As utilize its characteristic that adapts to low dissolved oxygen environment, be applied to sewage water denitrification and process the part power consumption that can save water factory; Utilize it to there is the characteristic of stronger competitive power under low ammonia nitrogen condition, be applied to, in the denitrogenation processing of Low Concentration Ammonia Containing Wastewater, can improve the denitrification effect of this type of waste water.
The accompanying drawing explanation
The typical curve of Fig. 1 ammonia oxidation archeobacteria amoA gene copy number
The typical curve of Fig. 2 ammonia oxidizing bacteria amoA gene copy number
The typical curve of Fig. 3 bacterium copy number
The typical curve of the ancient bacterium copy number of Fig. 4
Embodiment
Below in conjunction with accompanying drawing, preferably embodiment of the present invention is described in further detail:
Embodiment 1
The enrichment culture method of ammonia oxidation flora
(1) preparation of ammonia oxidation flora enrichment medium: NaCl 1g, MgCl
26H
2o 0.4g, KCl 0.5g, CaCl
20.1g, KBr 0.1g, KH
2pO
40.2g, water 1000mL.121 ℃ of lower sterilizing 30min, then add with the NH after 0.2 μ m membrane filtration
4cl(1M): 1mL, NaHCO
3solution (1M): 2mL, EDTA Na-Fe solution: 1mL, Sodium.alpha.-ketopropionate solution (1M): 1mL, trace element: 1mL, VITAMIN: 1mL, selenium tungsten solution: 1mL.
(2) enrichment culture of ammonia oxidation flora: will contain ammonia oxidation archeobacteria microbial film or active sludge and be inoculated in enrichment medium, regulating the pH value is 7.2-7.5, standing cultivation in the incubator that is 37 ± 1 ℃ in temperature, incubation time be take 14-16 days as generation culture cycle, cultivate 2 cycles in dark environment, and supplemented 1mLNH every 2-3 days
4cl solution and 2mLNaHCO
3solution.
The purification enrichment cultural method of ammonia oxidation archeobacteria
(3) enrichment medium of ammonia oxidation archeobacteria preparation: add penicillin: 50mg/L in the enrichment medium of ammonia oxidation flora, Streptomycin sulphate: 50mg/L, penbritin: 50mg/L.
(4) the enrichment culture method of ammonia oxidation archeobacteria: select 0.45 μ m film syringe-driven filter to filter ammonia oxidation flora enrichment culture liquid and then filtrate is inoculated in ammonia oxidation archeobacteria substratum.Regulating the pH value is 7.2-7.5, and standing cultivation in the incubator that is 37 ± 1 ℃ in temperature is cultivated 3 cycles in dark environment, and supplements 0.5mLNH every 3-5 days
4cl solution and 1mLNaHCO
3solution.Can obtain the ammonia oxidation archeobacteria enrichment culture liquid that purity is higher.
Embodiment 2
The enrichment culture method of ammonia oxidation flora
(1) preparation of enrichment medium: NaCl 2g, MgCl
26H
2o 0.8g, KCl 1g, CaCl
20.2g, KBr 0.2g, KH
2pO
40.4g, water 2000mL.121 ℃ of lower sterilizing 30min, then add with the NH after 0.2 μ m membrane filtration
4cl(1M): 2mL, NaHCO
3solution (1M): 4mL, EDTA Na-Fe solution: 2mL, Sodium.alpha.-ketopropionate solution (1M): 2mL, trace element: 2mL, VITAMIN: 2mL, selenium tungsten solution: 2mL.
(2) enrichment culture of ammonia oxidation flora: will contain ammonia oxidation archeobacteria microbial film or active sludge and be inoculated in enrichment medium, regulating the pH value is 7.2-7.5, standing cultivation in the incubator that is 37 ± 1 ℃ in temperature, incubation time be take 14-16 days as generation culture cycle, cultivate 2 cycles in dark environment, and supplemented 2mLNH every 2-3 days
4cl solution and 4mLNaHCO
3solution.
The purification enrichment cultural method of ammonia oxidation archeobacteria
(3) enrichment medium of ammonia oxidation archeobacteria preparation: add penicillin: 50mg/L in the enrichment medium of ammonia oxidation flora, Streptomycin sulphate: 50mg/L, penbritin: 50mg/L.
(4) the enrichment culture method of ammonia oxidation archeobacteria: select 0.45 μ m film syringe-driven filter to filter ammonia oxidation flora enrichment culture liquid and then filtrate is inoculated in ammonia oxidation archeobacteria substratum.Regulating the pH value is 7.2-7.5, and standing cultivation in the incubator that is 37 ± 1 ℃ in temperature is cultivated 3 cycles in dark environment, and supplements 1mLNH every 3-5 days
4cl solution and 2mLNaHCO
3solution.Can obtain the ammonia oxidation archeobacteria enrichment culture liquid that purity is higher.
Embodiment 3
The enrichment culture method of ammonia oxidation flora
(1) preparation of enrichment medium: NaCl 5g, MgCl
26H
2o 2g, KCl 2.5g, CaCl
20.5g, KBr 0.5g, KH
2pO
41g, water 5000mL.121 ℃ of lower sterilizing 30min, then add with the NH after 0.2 μ m membrane filtration
4cl(1M): 5mL, NaHCO
3solution (1M): 10mL, EDTA Na-Fe solution: 5mL, Sodium.alpha.-ketopropionate solution (1M): 5mL, trace element: 5mL, VITAMIN: 5mL, selenium tungsten solution: 5mL.
(2) enrichment culture of ammonia oxidation flora: microbial film or the active sludge that will contain the ammonia oxidation archeobacteria are inoculated in enrichment medium, regulating the pH value is 7.2-7.5, standing cultivation in the incubator that is 37 ± 1 ℃ in temperature, incubation time be take 14-16 days as generation culture cycle, cultivated for 2 culture cycles in dark environment, and supplemented 5mL NH every 2-3 days
4cl solution and 10mL NaHCO
3solution.
The purification enrichment cultural method of ammonia oxidation archeobacteria
(3) enrichment medium of ammonia oxidation archeobacteria preparation: add penicillin: 50mg/L in the enrichment medium of ammonia oxidation flora, Streptomycin sulphate: 50mg/L, penbritin: 50mg/L.
(4) the enrichment culture method of ammonia oxidation archeobacteria: select 0.45 μ m film syringe-driven filter to filter ammonia oxidation flora enrichment culture liquid and then filtrate is inoculated in ammonia oxidation archeobacteria substratum.Regulating the pH value is 7.2-7.5, and standing cultivation in the incubator that is 37 ± 1 ℃ in temperature was cultivated for 3 culture cycles in dark environment, and supplements 2.5mLNH every 3-5 days
4cl solution and 5mLNaHCO
3solution.Can obtain the ammonia oxidation archeobacteria enrichment culture liquid that purity is higher.
The main detection method of experiment is real-time fluorescence quantitative PCR, and the primer adopted is as shown in table 1 below, and the typical curve of doing is shown in Fig. 1-4.The typical curve of ammonia oxidizing bacteria is shown in Fig. 1, and detection line is 10
4copies/ μ L; The AOB typical curve is shown in Fig. 2, and detection line is 10 copies/ μ L; The typical curve of bacterium is shown in Fig. 3, and detection line is 10
4copies/ μ L; The typical curve of ancient bacterium is shown in Fig. 4, and detection line is 10
5copies/ μ L.
(1) reaction system: the PCR reaction system is 20 μ L, and reaction system is as follows: 10 μ LSYBR Premix Ex Taq II, and 2 μ L samples, each 0.4 μ L of upstream primer and downstream primer, all the other are supplied with aseptic RNase-free water.
(2) reaction conditions: 95 ℃ of warm start 30s at first, then carry out following 40 circulations: 95 ℃ of sex change 5s, 53 ℃ (ancient bacterium)/55 ℃ (ammonia oxidation archeobacteria, ammonia oxidizing bacteria)/60 ℃ (bacterium) annealing 30s, 72 ℃ are extended 1min, and 72 ℃ are read fluorescent value.
(3) measure solubility curve immediately between 65-95 ℃ after specific detection: PCR reaction finishes, and reaction product is also carried out to electrophoresis detection.The Tm value more than 80 ℃ and solubility curve show that measurement result is accurate while being unimodal.
In the enrichment culture liquid of gained, the be above standard largest loop number of curve of the cycle number that the ammonia oxidizing bacteria real-time fluorescence quantitative PCR records (CT value), the quantity of ammonia oxidizing bacteria is lower than detection line, sample to the ammonia oxidizing bacteria real-time fluorescence quantitative PCR carries out electrophoresis experiment, do not find the bright wisp band, the existence of ammonia-free oxidation bacterium in interpret sample.The quantity of ammonia oxidation archeobacteria by initial lower than 10
4copies/mL, less than 0.01% of total count, after enrichment culture, quantity reaches 10
7more than copies/mL, account for 17% left and right of total count, at least increased by 3 orders of magnitude, concentration effect is remarkable.Microflora after embodiment 1-3 enrichment culture is in Table 2.
Table 1 pcr amplification primer information
Annotate: K=G or T, S=G or C
Table 2
"-" means that ammonia oxidizing bacteria is lower than detection line
Above content is in conjunction with concrete preferred implementation further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For the general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.
Claims (10)
1. the method for ammonia oxidation archeobacteria enrichment culture in a Sewage treatment systems, is characterized in that, comprises following step:
A, preparation ammonia oxidation flora enrichment medium;
The enrichment culture of B, ammonia oxidation flora: microbial film or the active sludge that will contain the ammonia oxidation archeobacteria are inoculated in enrichment medium;
The enrichment medium of C, preparation ammonia oxidation archeobacteria: in the enrichment medium of ammonia oxidation flora, add microbiotic;
The enrichment culture of D, ammonia oxidation archeobacteria: after with strainer, enrichment culture liquid being filtered, filtrate is transferred in the ammonia oxidation archeobacteria enrichment medium in step C, obtains the enrichment culture liquid of the ammonia oxidation archeobacteria that purity is high.
2. the method for ammonia oxidation archeobacteria enrichment culture in Sewage treatment systems as claimed in claim 1, is characterized in that, in described steps A, enrichment medium comprises: NaCl, MgCl
26H
2o, KCl, CaCl
2, KBr, KH
2pO
4, NaCl, MgCl
26H
2o, KCl, CaCl
2, KBr and KH
2pO
4mass ratio be 10:4:5:1:1:2.
3. the method for ammonia oxidation archeobacteria enrichment culture in Sewage treatment systems as claimed in claim 1, it is characterized in that, in described steps A, the compound method of enrichment medium comprises: the solution in enrichment medium, at 121 ℃ of lower sterilizing 30min, is then added with the NH after 0.2 μ m membrane filtration
4cl solution, NaHCO
3solution, EDTA Na-Fe solution, Sodium.alpha.-ketopropionate solution, trace element, VITAMIN and selenium tungsten solution.
4. the method for ammonia oxidation archeobacteria enrichment culture in Sewage treatment systems as claimed in claim 3, is characterized in that, in affiliated steps A, and micro-compound method: 1.5g FeCl
24H
2o first is dissolved in 10mL HCl, then adds CoCl
26H
2o:190mg, MnCl
26H
2o:100mg, ZnCl
2: 70mg, HBO
3: 62mg, Na
2moO
42H
2o:36mg, NiCl
26H
2o:24mg, CuCl
22H
2o:17mg, add water and be settled to 1000mL;
Vitamin formula is: PABA: 40mg, and Bio: 10mg, nicotinic acid: 100mg, calcium D-VB5 calcium: 50mg, pyridoxine hydrochloride: 150mg, water 1000mL, then be 7.1 with 10mM buffer solution of sodium phosphate adjust pH;
Selenium tungsten solution formula: NaOH:0.4g, Na
2seO
35H
2o:6mg, Na
2wO
42H
2o:8mg, water 1000mL.
5. the method for ammonia oxidation archeobacteria enrichment culture in Sewage treatment systems as claimed in claim 1, is characterized in that, in described step B, the pH value is 7.2-7.5, standing cultivation in the incubator that is 37 ± 1 ℃ in temperature.
6. the method for ammonia oxidation archeobacteria enrichment culture in Sewage treatment systems as claimed in claim 1, it is characterized in that, in described step C, described microbiotic comprises penicillin, Streptomycin sulphate and penbritin, and the interpolation concentration of penicillin, Streptomycin sulphate and penbritin is 50mg/L.
7. the method for ammonia oxidation archeobacteria enrichment culture in Sewage treatment systems as claimed in claim 1, is characterized in that, in described step D, strainer adopts 0.45 μ m film syringe-driven filter.
8. the method for ammonia oxidation archeobacteria enrichment culture in Sewage treatment systems as claimed in claim 1, is characterized in that, in described step D, regulating the pH value is 7.2-7.5, standing cultivation in the incubator that is 37 ± 1 ℃ in temperature.
9. the method for ammonia oxidation archeobacteria enrichment culture in Sewage treatment systems as claimed in claim 1, is characterized in that, in described step B, incubation time be take 14-16 days as generation culture cycle, cultivates 2 cycles in dark environment, and supplemented NH every 2-3 days
4cl and NaHCO
3.
10. the method for ammonia oxidation archeobacteria enrichment culture in Sewage treatment systems as claimed in claim 1, is characterized in that, in described step D, in dark environment, cultivates 3 cycles, and supplemented NH every 3-5 days
4cl and NaHCO
3, obtain the ammonia oxidation archeobacteria enrichment culture liquid that purity is higher.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104593304A (en) * | 2015-01-21 | 2015-05-06 | 浙江大学 | Quick enrichment culturing method of ocean ammoxidation archaea |
| CN110616175A (en) * | 2019-10-25 | 2019-12-27 | 中国水产科学研究院渔业机械仪器研究所 | Ammonia oxidizing archaea and fresh water pond ammonia oxidizing archaea enrichment culture method |
| CN112029632A (en) * | 2020-09-04 | 2020-12-04 | 北京博瑞兴环境科技有限公司 | In-situ culture and enrichment device for anaerobic microorganisms in underground water and using method |
| CN113583887A (en) * | 2020-04-30 | 2021-11-02 | 中国科学院沈阳应用生态研究所 | Enrichment culture method of anaerobic flora for degrading pesticide 2,4-D |
| CN113860499A (en) * | 2021-10-20 | 2021-12-31 | 同济大学 | Low-ammonia-nitrogen-concentration sewage mainstream anaerobic ammonia oxidation system and process for limiting nitrobacteria by antibiotics |
| CN115259378A (en) * | 2022-08-25 | 2022-11-01 | 华南农业大学 | Method for enriching ammonia oxidizing archaea in sewage treatment system |
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| CN105907656B (en) * | 2016-03-24 | 2021-02-26 | 中国科学院城市环境研究所 | Culture method for prolonging passage time of ammonia oxidizing archaea |
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| CN104593304A (en) * | 2015-01-21 | 2015-05-06 | 浙江大学 | Quick enrichment culturing method of ocean ammoxidation archaea |
| CN110616175A (en) * | 2019-10-25 | 2019-12-27 | 中国水产科学研究院渔业机械仪器研究所 | Ammonia oxidizing archaea and fresh water pond ammonia oxidizing archaea enrichment culture method |
| CN110616175B (en) * | 2019-10-25 | 2021-03-26 | 中国水产科学研究院渔业机械仪器研究所 | Ammonia oxidizing archaea and fresh water pond ammonia oxidizing archaea enrichment culture method |
| CN113583887A (en) * | 2020-04-30 | 2021-11-02 | 中国科学院沈阳应用生态研究所 | Enrichment culture method of anaerobic flora for degrading pesticide 2,4-D |
| CN113583887B (en) * | 2020-04-30 | 2024-01-30 | 中国科学院沈阳应用生态研究所 | Enrichment culture method of anaerobic flora for degrading pesticide 2,4-D |
| CN112029632A (en) * | 2020-09-04 | 2020-12-04 | 北京博瑞兴环境科技有限公司 | In-situ culture and enrichment device for anaerobic microorganisms in underground water and using method |
| CN113860499A (en) * | 2021-10-20 | 2021-12-31 | 同济大学 | Low-ammonia-nitrogen-concentration sewage mainstream anaerobic ammonia oxidation system and process for limiting nitrobacteria by antibiotics |
| CN115259378A (en) * | 2022-08-25 | 2022-11-01 | 华南农业大学 | Method for enriching ammonia oxidizing archaea in sewage treatment system |
| CN115259378B (en) * | 2022-08-25 | 2024-04-12 | 华南农业大学 | Method for enriching archaea ammoxidation bacteria in sewage treatment system |
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