CN103439488A - Complete equipment and detection method for rapidly detecting meat product safety on site - Google Patents
Complete equipment and detection method for rapidly detecting meat product safety on site Download PDFInfo
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- CN103439488A CN103439488A CN2013103926981A CN201310392698A CN103439488A CN 103439488 A CN103439488 A CN 103439488A CN 2013103926981 A CN2013103926981 A CN 2013103926981A CN 201310392698 A CN201310392698 A CN 201310392698A CN 103439488 A CN103439488 A CN 103439488A
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Abstract
The invention discloses complete equipment for rapidly detecting meat product safety on site. The complete equipment comprises a detection box and detection equipment arranged inside the detection box, wherein the detection box comprises a double-layer box body and a box cover which is movably connected with the box body; the detection equipment comprises sample pretreatment equipment, rapid colloidal gold detection cards, an experiment reagent, reading and data processing equipment and experiment materials. The complete equipment integrally combines all equipment, reagents and materials required in safety detection on meat products, and is applicable to on-site rapid screening and detection on antibiotics and prohibited veterinary drugs in the meat product, so that the purpose that harmful substances can be discovered, monitored and managed in time is achieved; moreover the complete equipment is convenient to carry and anti-knock and anti-drop, and the function of a mobile laboratory is achieved.
Description
Technical field
The present invention relates to a kind of checkout equipment that detects meat products safety, particularly relate to a kind of set of equipments that can field quick detection meat products safety, it integrates sample pre-treatments equipment, detects reagent, test card, consumptive material, reads and data processing equipment, and the on-site quick screening that is applicable to meat products safety detects.
Background technology
China's meat products industry development is rapid, and the outstanding problem but microbiotic, violated veterinary drug exceed standard etc., seriously restrict China's animal husbandry development and jeopardize the healthy of consumer.Traditional instrumental method is as chromatography or chromatograph-mass spectrometer coupling technology assay method is loaded down with trivial details, efficiency is low, is not suitable for the fast detecting of a large amount of samples.The ELISA method is to utilize the competitive immunization detection method of enzyme labeling thing, widely is used as the quick screening method of qualitative or half-quantitative detection in recent years, although shortened detection time to a certain extent, operating process is still more complicated.More main, above detection method is laboratory method, is difficult to apply at the scene, is unfavorable for promoting the use of.At present, detection to meat products not only has been confined to laboratory, but expand to the links such as production, cultivation, circulation, and the time sex-limited having relatively high expectations, therefore in the urgent need to complete checkout equipment and the analytical approach of portability, can substitute to a certain extent laboratory and detect, reach the effect of primary dcreening operation.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of set of equipments of simple in structure, field quick detection meat products safety that cost is low, easy and simple to handle.
A kind of set of equipments of field quick detection meat products safety, the checkout equipment that comprises detection case and section placed within, described detection case comprise casing and with the case lid of its flexible connection, described checkout equipment comprises sample pre-treatments equipment, collaurum rapid detection card, experiment reagent, reads and data processing equipment and experiment consumptive material;
The set of equipments of field quick detection meat products safety of the present invention, the internal cavities that wherein said casing and case lid form respectively is filled with the supporting mould of novel environment friendly plastics expanded material material, be provided with the consistent locating slot of same checkout equipment shape on the surface of described supporting mould, inlay respectively and hold described checkout equipment.Described supporting mould, according to the placing articles shape machine-shaping of inner institute, can play a protective role to equipment in casing.This novel environment friendly plastics expanded material is compared with traditional material, has the advantages such as good buffering, antidetonation, heat insulation, moistureproof, resist chemical, and nontoxic, do not absorb water.
The set of equipments of field quick detection meat products safety of the present invention, wherein said sample pre-treatments equipment comprises that sample preparation is cut, sample preparation cutter, marking pen, sample spoon, micro balance, pipettor, single track timer, vortex centrifugal all-in-one, tissue sample multiplication of voltage rapid treatment instrument, full Self-controllable level indication nitrogen purge instrument;
Described collaurum rapid detection card comprises sulfamido test card, quinolones test card, chloromycetin test card, gentamicin test card, Clenbuterol test card, Ractopamine test card, salbutamol test card.
Described reading with data processing equipment comprises eight passage collaurum detector and work computer;
Described experiment consumptive material comprises emgloves, centrifuge tube, accurate pH test paper, rifle head, dropper, lab-gown.
The set of equipments of field quick detection meat products safety of the present invention, wherein,
On the supporting mould of described case lid internal cavities by from left to right, order from top to bottom is disposed with work computer locating slot, pipettor locating slot, sample preparation cutter locating slot, sample spoon locating slot, marking pen locating slot, sample preparation and cuts locating slot, micro balance locating slot and timer locating slot;
On the upper strata of described box house cavity supporting mould by from left to right, order from top to bottom is disposed with collaurum rapid detection card locating slot, vortex centrifugal all-in-one locating slot, eight passage collaurum detector locating slots, Sample Grinder locating slot; In lower floor's drawer on the supporting mould by from left to right, order from top to bottom is disposed with full Self-controllable level indication nitrogen and purges instrument locating slot, tissue sample multiplication of voltage rapid treatment instrument locating slot, experiment consumptive material locating slot;
Wherein, described a plurality of locating slots hold corresponding checkout equipment, and described operation instructions are placed in described work computer locating slot together with work computer.Adopt specific order and the mode of putting of the present invention, various required instrument consumptive materials are very clear, conveniently fetch and operate, and can effectively improve detection efficiency.
The set of equipments of field quick detection meat products safety of the present invention, one side of wherein said casing also comprises wheel body, opposite side corresponding thereto is provided with telescopic lever, described wheel body is that 4 polyurethane increase the weight of roller, can bear larger weight, volume is small and exquisite, and conveniently moving can move to rapidly destination and carry out Site Detection.
The set of equipments of field quick detection meat products safety of the present invention, wherein said detection case is made by the polypropylene composite material, the eucaryotic cell structure of described polypropylene composite material is for opening nucleus, between each cell, interlock mutually, open the nucleus structural design, lightweight, intensity is high, firm solid, another advantage is effectively to absorb external force so good damping effect; Also be provided with a plurality of dual control snap closes on the casing of described detection case or case lid, be easy to the open closed casing, in the situation that soil is arranged, have and huskyly open, shut, traditional design is difficult to shut as snap close enters dust.
The set of equipments of field quick detection meat products safety of the present invention, wherein said dual control snap close is 7, evenly is arranged on the edge of described case lid.
The set of equipments of field quick detection meat products safety of the present invention, wherein also be provided with O-ring seal at the edge of described case lid, and obturation effect is good; Described detection case also comprises gas balance valve, regulates inside and outside differential pressure, and under plateau, high altitude environment, air can be entered, and hydrone can not be entered, protection against the tide.
The application that microbiotic, violated veterinary drug exceed standard in the fast detecting meat products at the scene of the set of equipments of field quick detection meat products safety of the present invention.
A kind of method that set of equipments that adopts described field quick detection meat products safety is detected, comprise the steps:
(1) first complete reading operation instructions before being tested, and test card and sample to be checked are returned to room temperature;
(2) take out the collaurum rapid detection card from the original packing bag;
(3) use the sample extraction instrument, sampling, marking pen mark; Take 1-5g in centrifuge tube after organizing sample to pulverize, add sample extracting solution, mix with the vortex centrifugal all-in-one, the centrifugal 10min of 4000r/min, get supernatant, to be checked;
Described extracting tool comprises that sample preparation is cut, sample preparation cutter, sample spoon and micro balance, Sample Grinder;
(4) the collaurum rapid detection card is kept flat, with pipettor or dropper, draw the sample solution to be checked after processing, vertically dropwise add 5-8 to drip in well, start to use the timer timing after application of sample;
After (5) 5 minutes, test card is put into to eight passage collaurum detector reading out datas, with notebook computer, carried out the data processing.
The set of equipments difference from prior art of field quick detection meat products safety of the present invention is:
1, set of equipments accessory configuration of the present invention is complete, by sample pre-treatments equipment, detects reagent, test card, read with data processing equipment and integrate, be applicable to the on-site quick screening of meat products safety, thereby reach, hazardous material mass-energy is found and monitoring management in time.
2, sample pre-treatments equipment of the present invention is for Site Detection, to develop specially, has miniaturization, the advantage such as portable, easy to use;
3, using-system sample multiplication of voltage rapid treatment instrument of the present invention, extruding design fast processing animal tissue sample, improve sample pre-treatments efficiency.
4, the present invention uses the vortex centrifugal all-in-one to carry out the vortex of sample and centrifugal, and dual-use is saved space and cost in casing.
5, the present invention uses full Self-controllable level indication nitrogen blow device to carry out sample concentration, by the liquid-level tracking technology, has improved nitrogen and has blown efficiency, has saved nitrogen use level, has saved nitrogen and has blown out this.
6, set of equipments of the present invention, the microbiotic in the fast detecting meat products, violated residue of veterinary drug, detection time 5-10 minute, detectability meets the limit standard of country and European Union.
7, for the experiment place, do not require, can carry out the work in any place, the analytical test of related object is carried out at the scene of being adapted to, and the rapid screening that can be used for quality supervised department spot sampling check, enterprise inspection by oneself, border port detects, the compartmentalization management and control pattern that developed country implements.
8, simple to operate, common experimenter's by specification can operate.
9, the design of detection case wheel body and telescopic lever makes it easy to carry, and described wheel body is that 4 polyurethane increase the weight of roller, can bear larger weight, and volume is small and exquisite, and conveniently moving can move to rapidly destination and carry out Site Detection.Described detection case is made by the polypropylene composite material, the eucaryotic cell structure of described polypropylene composite material is for opening nucleus, interlock mutually between each cell, open the nucleus structural design, lightweight, intensity is high, firm solid, another advantage is effectively to absorb external force, so the antidetonation shatter-resistant is effective; Also be provided with a plurality of dual control snap closes on the casing of described detection case or case lid, be easy to the open closed casing, in the situation that soil is arranged, have and huskyly open, shut, traditional design is difficult to shut as snap close enters dust.Edge at described case lid also is provided with O-ring seal, and obturation effect is good; Described detection case also comprises gas balance valve, regulates inside and outside differential pressure, and under plateau, high altitude environment, air can be entered, and hydrone can not be entered, protection against the tide.
10, described supporting mould is according to the placing articles shape machine-shaping of inner institute; can play a protective role to equipment in casing; this novel environment friendly plastics expanded material is compared with traditional material, has the advantages such as good buffering, antidetonation, heat insulation, moistureproof, resist chemical, and nontoxic, do not absorb water.
11, casing of the present invention is bilevel drawer-type structure, in described casing, all detecting instruments is adopted specifically to put sequentially and is put, and various required instrument consumptive materials are very clear, conveniently fetch and operate, and can effectively improve detection efficiency.
Set of equipments below in conjunction with accompanying drawing to field quick detection meat products safety of the present invention is described further.
The accompanying drawing explanation
Fig. 1 is the described detection case structural representation in open mode under one embodiment of the present invention.
Fig. 2 is dual control buckle structure schematic diagram of the present invention;
The cramp that Fig. 3 is dual control snap close of the present invention and the fixture block structural representation under opening.
Embodiment
As shown in Figure 1, a kind of set of equipments of field quick detection meat products safety, the checkout equipment that comprises detection case and section placed within, described detection case comprise casing 2 and with the case lid 1 of its flexible connection, preferably, described casing 2 is that a side is connected and is flexibly connected with case lid 1, can freely open and fasten; Described casing 2 and case lid 1 can reach the purpose of fastening in many ways, as the edge except connecting side arranges slide fastener, or the latch mechanism locking that cooperatively interacts are set.Lower floor is moveable drawer 3, on described drawer 3, is provided with hand grip portion, can freely take out and push away, and at the casing 2 movable drawer structures 3 of lower floor and 2 of casings, is provided with latch mechanism, and after pushing described lower floor drawer 3, drawer 3 is stated in the fastening lock residence.Described latch mechanism is any structure that can reach the locking purpose, as be dual control snap close of the present invention.
The described checkout equipment of Fig. 1 comprises sample pre-treatments equipment, collaurum rapid detection card, experiment reagent, reads and data processing equipment and experiment consumptive material, the internal cavities that the levels of described casing 2 and case lid 1 form respectively is filled with the supporting mould of novel environment friendly plastics expanded material material, be provided with the consistent locating slot of same checkout equipment shape on the surface of described supporting mould, inlay respectively and hold described checkout equipment.Described sample pre-treatments equipment comprises that sample preparation is cut, sample preparation cutter, sample spoon, micro balance, pipettor, single track timer, vortex centrifugal all-in-one, tissue sample multiplication of voltage rapid treatment instrument, full Self-controllable level indication nitrogen purge instrument; Described collaurum rapid detection card comprises sulfamido test card, quinolones test card, chloromycetin test card, gentamicin test card, Clenbuterol test card, Ractopamine test card, salbutamol test card; Described reading with data processing equipment comprises eight passage collaurum detector and work computer; Described experiment consumptive material comprises emgloves, centrifuge tube, accurate pH test paper, rifle head, dropper, lab-gown.
Preferably, on the supporting mould of described case lid 1 internal cavities of Fig. 1 by from left to right, order from top to bottom is disposed with work computer locating slot 4, pipettor locating slot 5, sample preparation cutter locating slot 6, sample spoon locating slot 7, marking pen locating slot 8, sample preparation and cuts locating slot 9, micro balance locating slot 10 and timer locating slot 11; On the supporting mould of described casing 2 upper strata internal cavities by from left to right, order from top to bottom is disposed with collaurum rapid detection card locating slot 12, vortex centrifugal all-in-one locating slot 13, eight passage collaurum detector locating slots 14, Sample Grinder locating slot 15; In lower floor's drawer of described casing 2 internal cavities on the supporting mould by from left to right, order from top to bottom is disposed with full Self-controllable level indication nitrogen and purges instrument locating slot 16, tissue sample multiplication of voltage rapid treatment instrument locating slot 17, experiment consumptive material locating slot 18; Wherein, described a plurality of locating slots hold corresponding checkout equipment, and described operation instructions are placed in described work computer locating slot 4 together with work computer.
The set of equipments of field quick detection meat products safety of the present invention, wherein a side of the described casing 2 of Fig. 1 also comprises the wheel body (not shown), opposite side corresponding thereto is provided with the telescopic lever (not shown).Volume is small and exquisite, and conveniently moving can move to rapidly destination and carry out Site Detection.Described wheel body is that 4 polyurethane increase the weight of roller, can bear larger weight, and volume is small and exquisite, and conveniently moving can move to rapidly destination and carry out Site Detection.Described detection case is made by the polypropylene composite material, and the eucaryotic cell structure of described polypropylene composite material is for opening nucleus, interlock mutually between each cell, open the nucleus structural design, lightweight, intensity is high, firm solid, another advantage is effectively to absorb external force so good damping effect.As shown in Figures 2 and 3, also be provided with a plurality of dual control snap closes 20 on the casing 2 of described detection case or case lid 1, preferably, 7 dual control snap closes 20 evenly are arranged on the edge of described case lid 1.Described dual control snap close 20 comprises hinged cramp 21 and fixture block 22, and the edge of described casing 2 is provided with the projection 24 adapted with described cramp 21, and described projection 24 is arranged on a circle of described casing 2 top; Described cramp 21 and described projection 24 fasten closes described detection case, close backward described casing 2 directions and promote described fixture block 22, make to fasten and more close, described cramp 21 and fixture block 22 under opening as shown in Figure 3, under buckling state as shown in Figure 2, in the situation that soil is arranged, have and huskyly open, shut, traditional design is difficult to shut as snap close enters dust; Also be respectively arranged with the through hole 23 passed for lockset on a side of described dual control snap close 20 and casing corresponding thereto 2, lockset through described through hole 23 by described casing 2 and case lid 1 locking.Edge at described case lid 1 also is provided with O-ring seal, and obturation effect is good; Described detection case also comprises gas balance valve 19, regulates inside and outside differential pressure, and under plateau, high altitude environment, air can be entered, and hydrone can not be entered, protection against the tide.
The application that microbiotic, violated veterinary drug exceed standard in the fast detecting meat products at the scene of the set of equipments of field quick detection meat products safety of the present invention.
A kind of method that set of equipments that adopts described field quick detection meat products safety is detected, comprise the steps:
(1) first complete reading operation instructions before being tested, and test card and sample to be checked are returned to room temperature;
(2) take out the collaurum rapid detection card from the original packing bag;
(3) use the sample extraction instrument, sampling, marking pen mark; Take 1-5g in centrifuge tube after organizing sample to pulverize, add sample extracting solution, mix with the vortex centrifugal all-in-one, the centrifugal 10min of 4000r/min, get supernatant, to be checked;
Described extracting tool comprises that sample preparation is cut, sample preparation cutter, sample spoon and micro balance, Sample Grinder;
(4) the collaurum rapid detection card is kept flat, with pipettor or dropper, draw the sample solution to be checked after processing, vertically dropwise add 5-8 to drip in well, start to use the timer timing after application of sample;
After (5) 5 minutes, test card is put into to eight passage collaurum detector reading out datas, with notebook computer, carried out the data processing.Embodiment 3
A kind of set of equipments that adopts described field quick detection meat products safety detects the method for hydrochloric acid Ke Lunte in the pork product, comprises the steps:
(1) first complete reading operation instructions before being tested, and test card and sample to be checked are returned to room temperature;
(2) take out the clenobuterol hydrochloride test card from the original packing bag;
(3) take fresh pork 1-5g with micro balance, put into tissue sample multiplication of voltage rapid treatment instrument and squeeze out sample liquids, with the centrifugal 10min of vortex centrifugal all-in-one 4000r/min, get supernatant, to be checked;
(5) described clenobuterol hydrochloride test card is kept flat, with pipettor or dropper, draw the sample solution to be checked after processing, vertically dropwise add 5-8 to drip in well, start timing after application of sample;
After (6) 5 minutes, test card is put into to eight passage collaurum detector reading out datas, with notebook computer, carried out the data processing.
A kind of set of equipments that adopts described field quick detection meat products safety detects the method for chloromycetin in the pork product, comprises the steps:
(1) first complete reading operation instructions before being tested, and test card and sample to be checked are returned to room temperature;
(2) take out the chloromycetin test card from the original packing bag;
(3) take the pork sample after 1-5g pulverizes, add sample extracting solution, mix the centrifugal 10min of 4000r/min with the vortex centrifugal all-in-one; Get supernatant 1mL in the 10mL centrifuge tube, use 50 ℃ of left and right evaporates to dryness of full Self-controllable level indication nitrogen blow device; Add 500 μ L redissolution liquid, the vortex vibration, and constantly draw flushing with dropper, until it is residual to dissolve tube wall fully; To be checked.
(4) described chloromycetin test card is kept flat, with pipettor or dropper, draw the sample solution to be checked after processing, vertically dropwise add 5-8 to drip in well, start timing after application of sample;
After (5) 5 minutes, test card is put into to eight passage collaurum detector reading out datas, with notebook computer, carried out the data processing.
Above-described embodiment is described the preferred embodiment of the present invention; not scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that those of ordinary skills make technical scheme of the present invention, all should fall in the definite protection domain of the claims in the present invention book.
Claims (10)
1. the set of equipments of a field quick detection meat products safety, it is characterized in that: the checkout equipment that comprises detection case and section placed within, described detection case comprise double-layer box body and with the case lid of its flexible connection, described checkout equipment comprises sample pre-treatments equipment, collaurum rapid detection card, experiment reagent, reads and data processing equipment and experiment consumptive material.
2. the set of equipments of field quick detection meat products safety according to claim 1, it is characterized in that: described casing is upper and lower double-layer structure, wherein lower floor is drawer-type structure; Levels structure and the case lid inside of described casing form respectively the glove cavity, wherein be filled with the supporting mould of novel environment friendly plastics expanded material material, be provided with the consistent locating slot of same checkout equipment shape on the surface of described supporting mould, inlay respectively and hold described checkout equipment.
3. the set of equipments of field quick detection meat products safety according to claim 1 and 2 is characterized in that: described sample pre-treatments equipment comprises that sample preparation is cut, sample preparation cutter, marking pen, sample spoon, operation instructions, micro balance, pipettor, single track timer, vortex centrifugal all-in-one, tissue sample multiplication of voltage rapid treatment instrument, full Self-controllable level indication nitrogen purge instrument;
Described collaurum rapid detection card comprises sulfamido test card, quinolones test card, chloromycetin test card, gentamicin test card, Clenbuterol test card, Ractopamine test card, salbutamol test card;
Described reading with data processing equipment comprises eight passage collaurum detector and work computer;
Described experiment consumptive material comprises emgloves, centrifuge tube, accurate pH test paper, rifle head, dropper, lab-gown.
4. the set of equipments of field quick detection meat products safety according to claim 3 is characterized in that: on the supporting mould of described case lid internal cavities by from left to right, order from top to bottom is disposed with work computer locating slot, pipettor locating slot, sample preparation cutter locating slot, sample spoon locating slot, marking pen locating slot, sample preparation and cuts locating slot, micro balance locating slot and timer locating slot;
On the supporting mould of described box house upper strata cavity by from left to right, order from top to bottom is disposed with collaurum rapid detection card locating slot, vortex centrifugal all-in-one locating slot, eight passage collaurum detector locating slots, Sample Grinder locating slot; On the supporting mould of lower floor's drawer-type structure internal cavity by from left to right, order from top to bottom is disposed with full Self-controllable level indication nitrogen and purges instrument locating slot, tissue sample multiplication of voltage rapid treatment instrument locating slot, experiment consumptive material locating slot;
Wherein, described a plurality of locating slots hold corresponding checkout equipment, and described operation instructions are placed in described work computer locating slot together with work computer.
5. the set of equipments of field quick detection meat products safety according to claim 4, it is characterized in that: a side of described casing also comprises wheel body, and opposite side corresponding thereto is provided with telescopic lever, described wheel body is that 4 polyurethane increase the weight of roller.
6. the set of equipments of field quick detection meat products safety according to claim 5, it is characterized in that: described detection case is made by the polypropylene composite material, the eucaryotic cell structure of described polypropylene composite material is for opening nucleus, interlock mutually between each cell; Also be provided with a plurality of dual control snap closes on the casing of described detection case or case lid.
7. the set of equipments of field quick detection meat products safety according to claim 6, it is characterized in that: described dual control snap close is 7, evenly is arranged on the edge of described case lid.
8. the set of equipments of field quick detection meat products safety according to claim 7, it is characterized in that: the edge at described case lid also is provided with O-ring seal; Described detection case also comprises gas balance valve.
9. the set of equipments of the described field quick detection meat products of the claim 1-8 safety application of microbiotic, violated residue of veterinary drug in the fast detecting meat products at the scene.
10. the method that the set of equipments that adopts the described field quick detection meat products of any claim safety in claim 1-8 is detected, is characterized in that: comprise the steps:
(1) first complete reading operation instructions before being tested, and test card and sample to be checked are returned to room temperature;
(2) take out the collaurum rapid detection card from the original packing bag;
(3) use the sample extraction instrument, sampling, marking pen mark; Take 1-5g in centrifuge tube after organizing sample to pulverize, add sample extracting solution, mix with the vortex centrifugal all-in-one, the centrifugal 10min of 4000r/min, get supernatant, to be checked;
Described extracting tool comprises that sample preparation is cut, sample preparation cutter, sample spoon and micro balance, Sample Grinder;
(4) the collaurum rapid detection card is kept flat, with pipettor or dropper, draw the sample solution to be checked after processing, vertically dropwise add 5-8 to drip in well, start to use the timer timing after application of sample;
After (5) 5 minutes, test card is put into to eight passage collaurum detector reading out datas, with notebook computer, carried out the data processing.
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