CN103439471A - Freezing-unfreezing circulation-based freshness detection method for freshwater fish slices - Google Patents
Freezing-unfreezing circulation-based freshness detection method for freshwater fish slices Download PDFInfo
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Abstract
The invention discloses a freezing-unfreezing circulation-based freshness detection method for freshwater fish slices. The method adopts a texture analyzer TPA mode to measure the textural parameters (elasticity, adhesiveness, chewiness and resilience) of freshwater fish slices subjected to freezing-unfreezing circulation treatment; through correlation analysis and rear linear regression analysis, a regression equation of the freezing-unfreezing circulation-based freshness detection method for freshwater fish slices is obtained, then the textural parameters of the fish slices to be tested are substituted into the regression equation to determine the TVB-N value of the fish slices to be tested to represent the freshness quality of the freshwater fish slices. The freezing-unfreezing circulation-based freshness detection method overcomes the triviality in measuring the TVB-N value, the freshness of the fish slices can be measured only by utilizing the texture analyzer, the detection time is short and the detection charge is low.
Description
Technical field
The present invention relates to aquatic products freshness Quality Detection field, particularly a kind of fish of fresh water fish slices Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation.
Background technology
Along with the development of economy and scientific and technological level, China's fishery has realized great-leap-forward development, and fresh-water fishes produce and also obtained develop rapidly, and output continues to increase, and especially the output value of China four large Chinese carps is surged.The caused food-safety problem of thing followed freshwater fish also constantly occurs.Along with improving constantly of people's living standard, requirement to fish food safety is more and more stricter, on market, general employing is freezing is simultaneously sold fillet with partitioning scheme, therefore, set up freeze-thaw fish of fresh water fish slices Noninvasive Measuring Method of Freshness, for production, processing, circulation and the consumption of fresh-water fishes processing enterprise, vital meaning is arranged.
The factor that affects the fish of fresh water fish slices freshness is a lot, is mainly concerned with the variation of physical property, chemical property, biochemical property.Flesh of fish texture characteristic mainly comprises hardness, elasticity, stickability, deadlocked property, chewiness and recovery, and it is the important indicator of reaction flesh of fish physical property.The variation of meat quality of fish structure is mainly that the variation due to the degraded of troponin matter and retention ability causes.Total volatile basic nitrogen (TVB-N) is to oppress chemical index important in putrid and deteriorated process, the palliating degradation degree of key reaction fish meat protein and the pollution level of microorganism, the variation of the reaction fish freshness that this index can be appropriate.Therefore between the variation of meat quality of fish structure and TVB-N value, good relationship is arranged.
The forecasting shelf life method of 200810041497.6 1 kinds of freshwater fish goods of application number, mainly according to each Index Establishment freshwater fish product property change kinetics model, 201010180216.2 1 kinds of quick nondestructives of application number detect the method for freshness of freshwater fish, it is mainly the resistance value that adopts the voltammetric determination flesh of fish, these methods are subject to the impact of external condition, and testing result is not accurate enough.
Summary of the invention
Above-mentioned shortcoming and deficiency in order to overcome prior art, the object of the present invention is to provide a kind of fish of fresh water fish slices Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation, saved detection time, saved the measurement cost, and testing result is accurate.
Purpose of the present invention is achieved through the following technical solutions:
A kind of fish of fresh water fish slices Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation comprises the following steps:
(1) preparation is through the fish of fresh water fish slices sample of freeze-thaw circular treatment;
(2) utilize matter structure instrument TPA pattern to measure the texture characteristic parameter of fish of fresh water fish slices sample; Described texture characteristic parameter comprises elasticity X
elasticity, stickability X
stickability, chewiness X
chewinesswith recovery X
recovery;
(3) measure the TVB-N value Y of fish of fresh water fish slices sample according to Chinese Fishery industry standard SC/T3032-2007 " mensuration of total volatile basic nitrogen in fishery products "
tVB-N;
(4) the TVB-N value Y of the fish of fresh water fish slices sample that the texture characteristic parameter of the fish of fresh water fish slices sample recorded according to step (2) and step (3) record
tVB-N, utilize correlation analysis and rearmounted linear regression analysis, set up the fish of fresh water fish slices Noninvasive Measuring Method of Freshness regression equation based on the freeze-thaw circulation: Y
tVB-N=C
0+ aX
elasticity+ bX
stickability+ cX
chewiness-dX
recovery, wherein, C
0, a, b, c, d be constant, for its value difference of different fresh-water fishes;
(5) utilize matter structure instrument TPA pattern to measure the texture characteristic parameter of fish of fresh water fish slices sample to be measured, the texture characteristic parameter substitution step (4) of fish of fresh water fish slices sample to be measured is obtained, in the fish of fresh water fish slices Noninvasive Measuring Method of Freshness regression equation based on the freeze-thaw circulation, trying to achieve the TVB-N value of fish of fresh water fish slices sample to be measured.
When fresh-water fishes are grass carp, the coefficient C in equation
0=-203.069, a=265.566, b=48.116, c=4.131, d=189.054.
When fresh-water fishes are crucian, the coefficient C in equation
0=-210.011, a=276.432, b=47.568, c=4.139, d=191.008.
When fresh-water fishes are silver carp, the coefficient C in equation
0=-198.978, a=249.832, b=50.134, c=5.257, d=200.003.
The described preparation of step (1), through the fish of fresh water fish slices sample of freeze-thaw circular treatment, is specially:
Live fish is lethal, scale, go internal organ, decaptitate, truncate and skin, then be divided into fillet, with circulating water, rinse well, blot the residual water on fillet surface with thieving paper, the tygon freshness protection package of packing into sealing, and carry out the processing of freeze-thaw cycle for the treatment of; One of them freeze-thaw treatment cycle is :-20 ℃ of freezing 12h, 4 ℃ of 12h that thaw.
The present invention adopts matter structure instrument TPA pattern to measure matter structure parametric elasticity, stickability, chewiness and the recovery through the fish of fresh water fish slices of freeze-thaw circular treatment; Set up the regression equation between fillet freshness index TVB-N value and matter structure parameter index by correlation analysis and rearmounted linear regression analysis, the TVB-N value of then the matter structure parameter substitution regression equation of fillet sample to be measured being obtained to fillet to be measured characterizes the freshness quality of fish of fresh water fish slices.
Compared with prior art, the present invention has the following advantages and beneficial effect:
While adopting detection method of the present invention to carry out the fish freshness detection, without measuring again chemical index; Shorten detection time, and only need by matter structure instrument TPA pattern one-shot measurement pledge structure parameter: elasticity, deadlocked property, chewiness and recovery just can obtain the fish freshness index, and each sample detection time was about about 1 minute; Saved the measurement cost, except the instrument of experimental design, each detection does not need other fees.
The accompanying drawing explanation
The process flow diagram of the fish of fresh water fish slices Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation that Fig. 1 is embodiments of the invention.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
Embodiment 1
As shown in Figure 1, the grass carp sheet Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation of the present embodiment comprises the following steps:
(1) prepare the grass carp sheet sample through the freeze-thaw circular treatment: live grass carp is lethal, scale, go internal organ, decaptitate, truncate and skin, then be divided into fillet, with circulating water, rinse well, blot the residual water on fillet surface with thieving paper, the tygon freshness protection package of packing into sealing, and carry out the freeze-thaw cycle for the treatment of in 1 cycle and process, one of them freeze-thaw cycle for the treatment of cycle is :-20 ℃ of freezing 12h, 4 ℃ of 12h that thaw;
(2) utilize matter structure instrument TPA pattern to measure the texture characteristic parameter of grass carp sheet sample; Described texture characteristic parameter comprises elasticity X
elasticity, stickability X
stickability, chewiness X
chewinesswith recovery X
recovery;
(3) measure the TVB-N value Y of grass carp sheet sample according to Chinese Fishery industry standard SC/T3032-2007 " mensuration of total volatile basic nitrogen in fishery products "
tVB-N;
(4) the TVB-N value Y of the grass carp sheet sample that the texture characteristic parameter of the grass carp sheet sample recorded according to step (2) and step (3) record
tVB-N, utilize correlation analysis and rearmounted linear regression analysis, set up the grass carp sheet Noninvasive Measuring Method of Freshness regression equation based on the freeze-thaw circulation: Y
tVB-N=-203.069+265.566X
elasticity+ 48.116X
deadlocked property+ 4.131X
chewiness-189.054X
recovery;
(5) utilize matter structure instrument TPA pattern to measure the texture characteristic parameter of grass carp sheet sample to be measured, the texture characteristic parameter substitution step (4) of grass carp sheet sample to be measured is obtained, in the grass carp sheet Noninvasive Measuring Method of Freshness regression equation based on the freeze-thaw circulation, trying to achieve the TVB-N value of grass carp sheet sample to be measured.
Set the control group experiment simultaneously, according to the national standard method of this area, measure grass carp sample TVB-N value in control group.The method gained TVB-N value that result is the present embodiment is 10.72mg/100g, and control group gained TVB-N value is 10.44mg/100g, and relative error is 2.61%.As can be seen here, two kinds of experimental data indifferences that method obtains, be the one-level freshness.
Embodiment 2
The crucian sheet Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation of the present embodiment comprises the following steps:
(1) prepare the fish of fresh water fish slices sample through the freeze-thaw circular treatment: live crucian is lethal, scale, go internal organ, decaptitate, truncate and skin, then be divided into fillet, with circulating water, rinse well, blot the residual water on fillet surface with thieving paper, the tygon freshness protection package of packing into sealing, and carry out the freeze-thaw cycle for the treatment of in 2 cycles and process, one of them freeze-thaw cycle for the treatment of cycle is :-20 ℃ of freezing 12h, 4 ℃ of 12h that thaw;
(2) utilize matter structure instrument TPA pattern to measure the texture characteristic parameter of crucian sheet sample; Described texture characteristic parameter comprises elasticity X
elasticity, stickability X
stickability, chewiness X
chewinesswith recovery X
recovery;
(3) measure the TVB-N value Y of fish of fresh water fish slices sample according to Chinese Fishery industry standard SC/T3032-2007 " mensuration of total volatile basic nitrogen in fishery products "
tVB-N;
(4) the TVB-N value Y of the crucian sheet sample that the texture characteristic parameter of the crucian sheet sample recorded according to step (2) and step (3) record
tVB-N, utilize correlation analysis and rearmounted linear regression analysis, set up the crucian sheet Noninvasive Measuring Method of Freshness regression equation based on the freeze-thaw circulation: Y
tVB-N=-210.011+276.432X
elasticity+ 47.568X
deadlocked property+ 4.139X
chewiness-191.008X
recovery;
(5) utilize matter structure instrument TPA pattern to measure the texture characteristic parameter of crucian sheet sample to be measured, the texture characteristic parameter substitution step (4) of crucian sheet sample to be measured is obtained, in the crucian sheet Noninvasive Measuring Method of Freshness regression equation based on the freeze-thaw circulation, trying to achieve the TVB-N value of crucian sheet sample to be measured.
Set the control group experiment simultaneously, according to the national standard method of this area, measure crucian sample TVB-N value in control group.The method gained TVB-N value 15.57mg/100g that result is the present embodiment, control group gained TVB-N value is 16.01mg/100g, relative error is 2.82%.As can be seen here, two kinds of experimental data indifferences that method obtains, be the secondary freshness.
Embodiment 3
The silver carp sheet Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation of the present embodiment comprises the following steps:
(1) prepare the silver carp sheet sample through the freeze-thaw circular treatment: the silver carp that will live is lethal, scale, go internal organ, decaptitate, truncate and skin, then be divided into fillet, with circulating water, rinse well, blot the residual water on fillet surface with thieving paper, the tygon freshness protection package of packing into sealing, and carry out the freeze-thaw cycle for the treatment of in 3 cycles and process, one of them freeze-thaw cycle for the treatment of cycle is :-20 ℃ of freezing 12h, 4 ℃ of 12h that thaw;
(2) utilize matter structure instrument TPA pattern to measure the texture characteristic parameter of silver carp sheet sample; Described texture characteristic parameter comprises elasticity X
elasticity, stickability X
stickability, chewiness X
chewinesswith recovery X
recovery;
(3) measure the TVB-N value Y of silver carp sheet sample according to Chinese Fishery industry standard SC/T3032-2007 " mensuration of total volatile basic nitrogen in fishery products "
tVB-N;
(4) the TVB-N value Y of the silver carp sheet sample that the texture characteristic parameter of the silver carp sheet sample recorded according to step (2) and step (3) record
tVB-N, utilize correlation analysis and rearmounted linear regression analysis, set up the silver carp sheet Noninvasive Measuring Method of Freshness regression equation based on the freeze-thaw circulation: Y
tVB-N=-198.978+249.832X
elasticity+ 50.134X
deadlocked property+ 5.257X
chewiness-200.003X
recovery;
(5) utilize matter structure instrument TPA pattern to measure the texture characteristic parameter of silver carp sheet sample to be measured, the texture characteristic parameter substitution step (4) of silver carp sheet sample to be measured is obtained, in the silver carp sheet Noninvasive Measuring Method of Freshness regression equation based on the freeze-thaw circulation, trying to achieve the TVB-N value of silver carp sheet sample to be measured.
Set the control group experiment simultaneously, according to the national standard method of this area, measure silver carp sample TVB-N value in control group.The method gained TVB-N value 19.59mg/100g that result is the present embodiment, control group gained TVB-N value is 19.86mg/100g, relative error is 1.38%.As can be seen here, two kinds of experimental data indifferences that method obtains, be the secondary freshness, but almost approach freshness critical value 20mg/100g.
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not limited by the examples; other any do not deviate from change, the modification done under Spirit Essence of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (5)
1. the fish of fresh water fish slices Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation, is characterized in that, comprises the following steps:
(1) preparation is through the fish of fresh water fish slices sample of freeze-thaw circular treatment;
(2) utilize matter structure instrument TPA pattern to measure the texture characteristic parameter of fish of fresh water fish slices sample; Described texture characteristic parameter comprises elasticity X
elasticity, stickability X
stickability, chewiness X
chewinesswith recovery X
recovery;
(3) measure the TVB-N value Y of fish of fresh water fish slices sample according to Chinese Fishery industry standard SC/T3032-2007 " mensuration of total volatile basic nitrogen in fishery products "
tVB-N;
(4) the TVB-N value Y of the fish of fresh water fish slices sample that the texture characteristic parameter of the fish of fresh water fish slices sample recorded according to step (2) and step (3) record
tVB-N, utilize correlation analysis and rearmounted linear regression analysis, set up the fish of fresh water fish slices Noninvasive Measuring Method of Freshness regression equation based on the freeze-thaw circulation: Y
tVB-N=C
0+ aX
elasticity+ bX
stickability+ cX
chewiness-dX
recovery; Wherein, C
0, a, b, c, d be constant, for its value difference of different fresh-water fishes;
(5) utilize matter structure instrument TPA pattern to measure the texture characteristic parameter of fish of fresh water fish slices sample to be measured, the texture characteristic parameter substitution step (4) of fish of fresh water fish slices sample to be measured is obtained, in the fish of fresh water fish slices Noninvasive Measuring Method of Freshness regression equation based on the freeze-thaw circulation, trying to achieve the TVB-N value of fish of fresh water fish slices sample to be measured.
2. the fish of fresh water fish slices Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation according to claim 1, is characterized in that, when fresh-water fishes are grass carp, and the coefficient C in equation
0=-203.069, a=265.566, b=48.116, c=4.131, d=189.054.
3. the fish of fresh water fish slices Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation according to claim 1, is characterized in that, when fresh-water fishes are crucian, and the coefficient C in equation
0=-210.011, a=276.432, b=47.568, c=4.139, d=191.008.
4. the fish of fresh water fish slices Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation according to claim 1, is characterized in that, when fresh-water fishes are silver carp, and the coefficient C in equation
0=-198.978, a=249.832, b=50.134, c=5.257, d=200.003.
5. the fish of fresh water fish slices Noninvasive Measuring Method of Freshness based on the freeze-thaw circulation according to claim 1, is characterized in that, the described preparation of step (1), through the fish of fresh water fish slices sample of freeze-thaw circular treatment, is specially:
Live fish is lethal, scale, go internal organ, decaptitate, truncate and skin, then be divided into fillet, with circulating water, rinse well, blot the residual water on fillet surface with thieving paper, the tygon freshness protection package of packing into sealing, and carry out the processing of freeze-thaw cycle for the treatment of; One of them freeze-thaw treatment cycle is :-20 ℃ of freezing 12h, 4 ℃ of 12h that thaw.
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Cited By (4)
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| CN105136709A (en) * | 2015-06-05 | 2015-12-09 | 华南理工大学 | Method and device for measuring freezing parameters of frozen meat |
| CN108254513A (en) * | 2017-12-22 | 2018-07-06 | 北京农业信息技术研究中心 | A kind of judgment method and device of fish shelf life |
| CN108896721A (en) * | 2018-07-05 | 2018-11-27 | 中国水产科学研究院黄海水产研究所 | A kind of rapid method for assessment for eating freezing tuna grade raw |
| CN111413475A (en) * | 2019-01-04 | 2020-07-14 | 中国农业大学 | Method for detecting physical properties of cold fresh mutton |
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| CN111413475A (en) * | 2019-01-04 | 2020-07-14 | 中国农业大学 | Method for detecting physical properties of cold fresh mutton |
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Application publication date: 20131211 |