CN103432588A - Copolymer for preparation of probiotic microcapsule and preparation method thereof - Google Patents
Copolymer for preparation of probiotic microcapsule and preparation method thereof Download PDFInfo
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- CN103432588A CN103432588A CN2013103858306A CN201310385830A CN103432588A CN 103432588 A CN103432588 A CN 103432588A CN 2013103858306 A CN2013103858306 A CN 2013103858306A CN 201310385830 A CN201310385830 A CN 201310385830A CN 103432588 A CN103432588 A CN 103432588A
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Abstract
The invention discloses a copolymer for preparation of a probiotic microcapsule and a preparation method thereof, which is characterized in that the copolymer is formed by polymerization of natural plant large molecules and cellulose ether or derivatives thereof; and the copolymer is prepared by polymerization of the following substances in percentage by dry weight: 10-50% of plant proteins, 10-70% of cellulose ether and derivatives thereof, 0-50% of plant polysaccharides, 0.01-5% of coupling agent, 0-3% of assistant and 0-3% of modifier. The copolymer is used for coating probiotic freeze-dried powder, can effectively protect probiotics, prolongs the survival time and stomach juice resistance of probiotics at room temperature, and enables the probiotics to safely reach the intestinal tract and other organs so as to exert the probiotic effect. The primary characteristics of the plant proteins, the plant polysaccharides and hydroxypropyl methylcellulose are basically kept in the preparation process of the copolymer; the product is high in safety and favorable in property; and the plant proteins in the invention are byproducts in agricultural product processing and low in cost.
Description
Technical field
The present invention relates to a kind of copolymer of preparing probiotic microcapsule and preparation method thereof that can be used for, belong to the technical fields such as prepared by probiotic composition.
Background technology
Probiotic bacteria is the active microorganism that a class is useful to the host, is to be colonizated in human body intestinal canal, reproductive system, thereby can produces the active beneficial microorganism general name that definite health efficacy improves host's microecological balance, performance beneficial effect.The health-care effect of probiotic bacteria may be summarized to be the following aspects: 1) whole intestinal effect maintains the intestinal microbial population balance, control diarrhoea; 2) alleviate lactose intolerance, promote the body alimentation; 3) the prevention vaginal infection, play favourable effect to health; 4) enhancing immunity; 5) reduce hypertension; 6) reduce serum cholesterol; 7) prophylaxis of cancer and inhibition tumor growth; 8) alleviate irritated effect etc.Because probiotic bacteria has treatment disease and prebiotic function, probiotic products has been widely used in the aspects such as food, cosmetics, medical treatment.Hygiene department has worked up and added probiotic bacteria planning in food, as Public nutrition, improves project.
When probiotic bacteria is widely applied, also to people, proposed some problems that can not be ignored: 1) strain is subject to the impact of the factors such as extraneous oxygen, light, heat, moisture and inactivation; 2) probiotic products is difficult to resist the impact of the factors such as stomach strong acid, high cholate and arrives the prebiotic effect of the normal performance of intestinal; 3) unstable product quality, at easy inactivations of process such as processing, storage, transportation, activation, cause the product viable count obviously to reduce.Therefore, how probiotic bacteria is processed, arrival intestinal that can be safe plays a role, and is current problem demanding prompt solution.Utilizing microcapsule embedded technical protection probiotic bacteria is the focus of studying both at home and abroad at present.By probiotic microcapsule, can solve preferably the deficiency that probiotic bacteria self exists, improve the performances such as its antioxygen, anti-gastric acid and enteric solubility, will certainly bring opportunity for the development and application of probiotic products.
Summary of the invention
The purpose of this invention is to provide a kind of copolymer for preparing probiotic microcapsule, for wrapping up Freeze-dry Powder of Probioctics, can effectively protect probiotic bacteria, improve stability and the storage property of probiotic products in production, storage process; Strengthen the gastric juice resistance, the protection probiotic bacteria organs such as intestinal that arrive safe and sound, bring into play prebiotic effect.
For achieving the above object, the present invention has taked following technical scheme:
A kind of probiotic microcapsule copolymer, so its characteristics are forming copolymer with natural plants macromole and the polymerization of cellulose ether or derivatives thereof; (the pressing the dry product weight percent meter) that described copolymer is formed by following material copolymerization: vegetable protein 10%～50%, cellulose ether and derivant 10%～70% thereof, vegetable polysaccharides 0～50%, coupling agent 0.01%～5%, auxiliary agent 0%～3%, modifier 0.01%～3%.
Described cellulose ether or derivatives thereof be from select one or more in group: hydroxypropyl emthylcellulose, ethyl cellulose, carboxymethyl cellulose, methylcellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose phthalate, hydroxypropyl methyl cellulose succinate.
Described vegetable protein be from select one or more in group: zein, wheat protein, soybean protein.
Described vegetable polysaccharides be from select one or more in group: pectin, arabic gum, mannan, guar gum, chitosan, starch, xanthan gum, trehalose.
Described modifier be from select one or more in group: stearic acid, lecithin, Span, Tween, transaminase.
Described coupling agent be from select one or more in group: ferulic acid, citric acid, gluconic acid, lactic acid, aminoacid.
Described auxiliary agent be from select one or more in group: glycerol, ethylene glycol, sorbitol.
A kind of method of probiotic microcapsule copolymer, comprise the steps, take at first by weight percentage each raw material, by cellulose ether or derivatives thereof dispersed with stirring 1h～4h in being preheated to the solvent of 60 ℃～100 ℃, slowly add vegetable polysaccharides that it is dissolved fully, continue to stir 1h～4h; Then the vegetable protein of dissolving is slowly joined in the hypromellose solution of stirring, after being uniformly dispersed, add coupling agent, auxiliary agent, modifier, stir, under the condition of 50 ℃～100 ℃, react 4h～8h, drying, packing, store.
This copolymer is the basic primary characteristic that keeps vegetable protein, vegetable polysaccharides, cellulose ether in preparation process, and Product Safety is high; The probiotic microcapsule good product performance of utilizing this copolymer to prepare, stable in properties; Vegetable protein in the present invention is the by-product in processing of farm products, with low cost.
The present invention abandons the animal raw materials such as gelatin with potential safety hazard, adopts natural plant material, and take vegetable protein, vegetable polysaccharides, cellulose ether is primary raw material, prepares the probiotic microcapsule copolymer, for the embedding Freeze-dry Powder of Probioctics.
Figure of description
Fig. 1 is embedding and the micro encapsulation bacillus acidophilus survival condition in the simulation simulated gastric fluid not in the specific embodiment.
The specific embodiment
Below by specific embodiment, technical solution of the present invention is further explained to explanation.
In following examples, material composition used unless otherwise noted, is commercially available prod.
Take the 7g hypromellose, be scattered in the 70mL pure water that is preheated to 80 ℃ and stir 2h; Take the 3g soybean protein and disperse in the 30mL water of 80 ℃, the sodium hydroxide adjust pH of 0.1N of take is 9, and boiling water is heat-treated to albumen and dissolves fully; Soy bean proteinous soln is slowly joined in hypromellose solution, after stirring 1h, by the gross mass of hypromellose and soybean protein, add successively ferulic acid 1%, glycerol 0.5%, sorbitol 0.5%, lecithin 0.1%, stir, copolyreaction 6h under the condition of 60 ℃; Glue be dry, pulverize, and packing, obtain the probiotic microcapsule copolymer.
Embodiment 2
Take respectively 7g hypromellose, 3g zein, glycine 0.1g mix homogeneously in small beaker; The alcoholic solution 100mL of preparation 80%, be heated to 60 ℃, slowly adds the powder of mix homogeneously, and it is dissolved fully; The 0.3g sorbitol dissolved is joined in above-mentioned solution, stir 1h; Add 0.5g glycerol, stir, copolyreaction 6h under the condition of 60 ℃; Glue be dry, pulverize, and packing, obtain the probiotic microcapsule copolymer.
Take the 7g hypromellose, be scattered in the 70mL pure water that is preheated to 80 ℃ and stir 2h; Take the 7g soybean protein and disperse in the 40mL water of 80 ℃, the sodium hydroxide adjust pH of 0.1N of take is 8, and boiling water is heat-treated to albumen and dissolves fully; Soy bean proteinous soln is slowly joined in hypromellose solution, after stirring 1h, by the gross mass of hypromellose and soybean protein, add successively ferulic acid 5%, glycerol 2.5%, sorbitol 0.5%, lecithin 0.01%, stir, copolyreaction 6h under the condition of 60 ℃; Glue be dry, pulverize, and packing, obtain the probiotic microcapsule copolymer.
Embodiment 4
Take respectively 7g hypromellose, 1g zein, glycine 0.1g mix homogeneously in small beaker; The alcoholic solution 110mL of preparation 80%, be heated to 60 ℃, slowly adds the powder of mix homogeneously, and it is dissolved fully; The 0.3g sorbitol dissolved is joined in above-mentioned solution, stir 1h, copolyreaction 6h under the condition of 60 ℃; Glue be dry, pulverize, and packing, obtain the probiotic microcapsule copolymer.
Take respectively 7g trehalose, 1g zein, 1g ethyl cellulose, 0.1g T-5398, mix homogeneously in small beaker; Slowly the powder of mix homogeneously is joined in the pure water that stirs 60 ℃ of 100mL, it is dissolved fully; The 0.3g sorbitol dissolved is joined in above-mentioned solution, stir 1h, copolyreaction 6h under the condition of 60 ℃; Glue be dry, pulverize, and packing, obtain the probiotic microcapsule copolymer.
In above embodiment: hypromellose also can be advanced with the arbitrary proportion combination to carry out the equivalent replacement by following one or more materials: ethyl cellulose, carboxymethyl cellulose, methylcellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose phthalate, hydroxypropyl methyl cellulose succinate.
Trehalose can carry out the equivalent replacement with the arbitrary proportion combination by following one or more materials: pectin, arabic gum, mannan, guar gum, chitosan, starch, xanthan gum.
Modifier lecithin can be take the arbitrary proportion combination by following one or more materials and be carried out the equivalent replacement: stearic acid, Span(Span), the Tween(mixture that is Tween 20 and the two Span 20s of a part of polyoxyethylene), the transaminase.
Coupling agent ferulic acid, aminoacid can carry out the equivalent replacement with the arbitrary proportion combination by following one or more materials: citric acid, gluconic acid, lactic acid.
Auxiliary agent glycerol, sorbitol can be replaced by ethylene glycol equivalent.
Above embodiment copolymer powder is soaked to 4h in the water of 40 ℃, stir, adopt the fluidized coating machine to be wrapped up the lactobacillus acidophilus freeze-drying powder, obtain performance good, the Lactobacillus acidophilus microcapsule product of stable in properties.
The copolymer powder of embodiment 1 is prepared to Lactobacillus acidophilus microcapsule as stated above, the gained sample is processed 2h in simulated gastric fluid, transfer in intestinal juice and process 3h, simulated the gastro-intestinal Fluid dissolution and investigated (simulated gastric fluid, intestinal juice are prepared with reference to Chinese Pharmacopoeia version in 2005), result as shown in Figure 1:
Road as shown in Figure 1, the bacillus acidophilus of embedding does not access in simulated gastric fluid, and absorbance reduces gradually, illustrates that the bacillus acidophilus's number in gastric juice constantly reduces.And the bacillus acidophilus after embedding discharges seldom in the stomach simulated solution, absorbance is very low, and the absorbance in intestinal juice increases gradually, and constantly stripping from embedding medium of bacillus acidophilus is described.Probiotic bacteria carries out micro encapsulation and can guarantee most of these digestive organs of probiotic bacteria safety stomach and arrive its physiological function of intestinal release performance.
Claims (8)
1. a probiotic microcapsule copolymer, it is characterized in that forming copolymer with natural plants macromole and the polymerization of cellulose ether or derivatives thereof; (the pressing the dry product weight percent meter) that described copolymer is formed by following material copolymerization: vegetable protein 10%～50%, cellulose ether and derivant 10%～70% thereof, vegetable polysaccharides 0～50%, coupling agent 0.01%～5%, auxiliary agent 0%～3%, modifier 0～3%.
2. probiotic microcapsule copolymer according to claim 1, wherein said cellulose ether or derivatives thereof be from select one or more in group: hydroxypropyl emthylcellulose, ethyl cellulose, carboxymethyl cellulose, methylcellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose phthalate, hydroxypropyl methyl cellulose succinate.
3. probiotic microcapsule copolymer according to claim 1, wherein said vegetable protein be from select one or more in group: zein, wheat protein, soybean protein.
4. probiotic microcapsule copolymer according to claim 1, wherein said vegetable polysaccharides be from select one or more in group: pectin, arabic gum, mannan, guar gum, chitosan, starch, xanthan gum, trehalose.
5. probiotic microcapsule copolymer according to claim 1, wherein said modifier be from select one or more in group: stearic acid, lecithin, Span, Tween, transaminase.
6. probiotic microcapsule copolymer according to claim 1, wherein said coupling agent be from select one or more in group: ferulic acid, citric acid, gluconic acid, lactic acid, aminoacid.
7. probiotic microcapsule copolymer according to claim 1, wherein said auxiliary agent be from select one or more in group: glycerol, ethylene glycol, sorbitol.
8. the method prepared as probiotic microcapsule copolymer as described in any one in claim 1-7, comprise the steps, at first by weight percentage take each raw material, by cellulose ether or derivatives thereof dispersed with stirring 1h～4h in being preheated to the solvent of 60 ℃～100 ℃, slowly add vegetable polysaccharides that it is dissolved fully, continue to stir 1h～4h; Then the vegetable protein of dissolving is slowly joined in the hypromellose solution of stirring, after being uniformly dispersed, add coupling agent, auxiliary agent, modifier, stir, under the condition of 50 ℃～100 ℃, react 4h～8h, drying, packing, store.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104351938A (en) * | 2014-10-21 | 2015-02-18 | 吉林农业大学 | Method for preparing food-grade enteric-soluble microcapsule |
| CN105295054A (en) * | 2015-11-16 | 2016-02-03 | 泰安瑞泰纤维素有限公司 | Preparation methods of hydroxypropyl methyl cellulose-soybean protein copolymer and its plant capsule |
| WO2017056075A1 (en) | 2015-10-01 | 2017-04-06 | Solutex Na, Lcc | Process for the preparation and stabilization of emulsions with omega-3 by means of isometric crystalline networks of cellulose derivatives |
| CN109846043A (en) * | 2017-11-30 | 2019-06-07 | 内蒙古伊利实业集团股份有限公司 | A kind of colon sustained release probiotics and preparation method thereof |
| CN112245409A (en) * | 2020-10-23 | 2021-01-22 | 安徽大学 | Vegetable protein-ursodesoxycholic acid sustained-release nanoparticle composite microcapsule and preparation method thereof |
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| CN102952280A (en) * | 2012-08-23 | 2013-03-06 | 安徽大学 | Hydroxypropyl methylcellulose copolymer for preparing plant capsules and preparation method thereof |
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| CN102952280A (en) * | 2012-08-23 | 2013-03-06 | 安徽大学 | Hydroxypropyl methylcellulose copolymer for preparing plant capsules and preparation method thereof |
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| 吴克刚: ""益生菌的生理功能及微胶囊化的必要性和方法"", 《第三届"益生菌、益生元与健康"研讨会专刊》 * |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104351938A (en) * | 2014-10-21 | 2015-02-18 | 吉林农业大学 | Method for preparing food-grade enteric-soluble microcapsule |
| WO2017056075A1 (en) | 2015-10-01 | 2017-04-06 | Solutex Na, Lcc | Process for the preparation and stabilization of emulsions with omega-3 by means of isometric crystalline networks of cellulose derivatives |
| US10856564B2 (en) | 2015-10-01 | 2020-12-08 | Solutex Na, Lcc | Process for the preparation and stabilization of emulsions with Omega-3 by means of isometric crystalline networks of cellulose derivatives |
| CN105295054A (en) * | 2015-11-16 | 2016-02-03 | 泰安瑞泰纤维素有限公司 | Preparation methods of hydroxypropyl methyl cellulose-soybean protein copolymer and its plant capsule |
| CN109846043A (en) * | 2017-11-30 | 2019-06-07 | 内蒙古伊利实业集团股份有限公司 | A kind of colon sustained release probiotics and preparation method thereof |
| CN109846043B (en) * | 2017-11-30 | 2023-04-07 | 内蒙古伊利实业集团股份有限公司 | Colon slow-release probiotics and preparation method thereof |
| CN112245409A (en) * | 2020-10-23 | 2021-01-22 | 安徽大学 | Vegetable protein-ursodesoxycholic acid sustained-release nanoparticle composite microcapsule and preparation method thereof |
| CN112245409B (en) * | 2020-10-23 | 2022-03-15 | 安徽大学 | Vegetable protein-ursodesoxycholic acid sustained-release nanoparticle composite microcapsule and preparation method thereof |
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Application publication date: 20131211 |