CN103432181B - Common pternopetalum root extract and new use thereof in pharmacy - Google Patents
Common pternopetalum root extract and new use thereof in pharmacy Download PDFInfo
- Publication number
- CN103432181B CN103432181B CN201310377422.6A CN201310377422A CN103432181B CN 103432181 B CN103432181 B CN 103432181B CN 201310377422 A CN201310377422 A CN 201310377422A CN 103432181 B CN103432181 B CN 103432181B
- Authority
- CN
- China
- Prior art keywords
- bisabolangelone
- sha
- jin
- extractive
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention relates to a new use of a common pternopetalum root extract, and particularly relates to the use of the common pternopetalum root extract in the field of pharmacy. The use concretely comprises the functions of treating and preventing dysmenorrheal, treating and preventing hypertension, treating and preventing atherosclerosis, treating and preventing cerebral thrombosis and the like caused by cerebral apoplexy. The common pternopetalum root extract is safe and non-toxic, and strong in pharmacologic action, and heralds a good medicinal prospect; meanwhile, the common pternopetalum root extract is simple in preparation process, and can be prepared into capsules, tablets, cream and oiling agents to be taken orally or used externally.
Description
Technical field
The present invention relates to a kind of novelty teabag of extractive of Zi-Jin-Sha, particularly relate to the purposes in pharmaceutical field.
Background technology
CN101002809A has disclosed extractive of Zi-Jin-Sha bisabolangelone, and its structural formula is:
This compound or mixture can be turned in the root of celery Angelica polymorpha Maxim. by Umbelliferae archangel and extract, and are distributed in Hubei, Hunan, Chongqing etc. and economize ground.There is effect of warming spleen and stomach for dispelling cold, regulating QI to relieve pain.Known can be used as treatment stomachache, stomachache, chest and hypochondrium, rheumatic arthritis, traumatic injury, venom etc.
Summary of the invention
The object of the present invention is to provide extractive of Zi-Jin-Sha and novelty teabag thereof, the new opplication namely in pharmacy.
The present invention realizes above-mentioned purpose like this: a kind of extractive of Zi-Jin-Sha, property raw material based on celery (Angelica polymorpha Maxim.) is turned by samphire, ethanol is extracting solution, and concentrating under reduced pressure obtains dark-brown extractum extract, then extracts obtained successively through organic solvent.Described concentration of alcohol is 40-100%, and in ethanol extraction, the percentage by weight of bisabolangelone is greater than 2%.Described organic solution is respectively petroleum ether, the chloroform of 95-98%, the ethyl acetate of 95-98%, the n-butyl alcohol of 95-98% of 95-98%, obtains petroleum ether part, chloroform extract, ethyl acetate extract, n-butanol portion respectively by organic solution successively ethanol extraction extract.Get ethyl acetate extract, frequent pressure column chromatography, with petroleum ether and ethyl acetate with volume ratio for 5-8:1, eluting repeatedly, recrystallization obtains compound bisabolangelone.
The invention further relates to extractive of Zi-Jin-Sha treat as preparation and prevent the novelty teabag in the medicine of dysmenorrhea.
Relate to extractive of Zi-Jin-Sha as the novelty teabag prepared in the medicine for the treatment of and prophylaxis of hypertension.
Relate to extractive of Zi-Jin-Sha as the novelty teabag in the medicine that preparation is treated and prevention of arterial is atherosis.
Relate to the novelty teabag in the medicine of the cerebral thrombosis that extractive of Zi-Jin-Sha causes as preparation treatment and preventing brain stroke.
The invention has the advantages that:
1) the present invention is to extractive of Zi-Jin-Sha and main constituent bisabolangelone, has excavated new medical usage, has opened up new application.
2) extractive of Zi-Jin-Sha safety non-toxic of the present invention, pharmacological action is strong, imply that good prospect in medicine.
3) extractive of Zi-Jin-Sha abundant raw material source of the present invention, inexpensive, have no toxic and side effects, preparation technology is simple, can make capsule, tablet, ointment, oil preparation for for oral administration or external.
4) extractive of Zi-Jin-Sha of the present invention has the effect of obviously treatment dysmenorrhea, and it significantly reduces contraction frequency, mean tension, average amplitude that oxytocin causes; Reduce CaCl
2the contraction frequency caused, mean tension, average amplitude; Significantly reduce mean tension, average amplitude and tension force maximum that KCl causes simultaneously; Also contraction frequency, mean tension and average amplitude that the oxytocin of rat Uterus in vivo causes is significantly reduced.
5) diseases such as the cerebral ischemia that extractive of Zi-Jin-Sha of the present invention has hypertension that obviously treatment is relevant to calcium antagonist, atherosclerosis, apoplexy cause.
Accompanying drawing explanation
The HPLC of Fig. 1 Radix Pternopetali vulgaris ethanol extraction analyzes collection of illustrative plates: wherein, chromatographic column XB-C
18(4.6mm × 250mm, 5 μm); Determined wavelength 248nm; Column temperature 25 DEG C; Mobile phase: (A) acetonitrile, (B) water, gradient elution, 0 ~ 23min (15% ~ 55%A, 45% ~ 85%B), 23 ~ 35min (55% ~ 70%A, 30% ~ 45%B), 35 ~ 45min (70% ~ 95%A, 5% ~ 30%B), 45 ~ 50min (95%A, 5%B); Sample size is 10 μ L, and flow velocity is 0.8mL/min.
The HPLC of Fig. 2 bisabolangelone analyzes collection of illustrative plates.
The HPLC of Fig. 3 petroleum ether part analyzes collection of illustrative plates.Wherein, main special characteristic component is: aqua oxidation peucedanin (retention time: 17.5min), bisabolangelone (retention time: 27min) and isoimperatorin (retention time: 36.5min).
The HPLC of Fig. 4 chloroform extract analyzes collection of illustrative plates.
The HPLC of Fig. 5 ethyl acetate extract analyzes collection of illustrative plates.
The HPLC of Fig. 6 n-butanol portion analyzes collection of illustrative plates.
Detailed description of the invention
In order to understand essence of the present invention better, its novelty teabag in pharmaceutical field will be described by the pharmacological testing of extractive of Zi-Jin-Sha and result below.
Take 3 kilograms, Radix Pternopetali vulgaris powder (40 order), with 95% ethanol respectively atmospheric pressure reflux extract three times, merge all extracting solution, recycling design, concentrating under reduced pressure obtains dark-brown extractum, with appropriate distilled water, extractum is suspended, extract with petroleum ether, chloroform, ethyl acetate, water saturated n-butyl alcohol successively.Add 9500mL extract and 500mL water mixes, standing 12h, after two phase stratification, utilizes siphonage principle to be separated out by the extract on upper strata, concentrates on a rotary evaporator and reclaim extract at every turn, and the solvent after recovery continues on for extraction.Merge the extract of each extractant, in fume hood, volatilize solvent, finally: petroleum ether part, chloroform extract, ethyl acetate extract, n-butanol portion, remaining aqueous solution part is get Shui position after the lyophilizing of frozen drying instrument, saves backup.
Get ethyl acetate extract and carry out normal pressure silica gel column chromatography in right amount, with petroleum ether-ethyl acetate (volume ratio is for 6:1) eluting repeatedly, recrystallization obtains compound bisabolangelone.Controlling bisabolangelone dosage is 1-10mg/kg, makes capsule, tablet, ointment, oil preparation for for oral administration or external.
Embodiment 1
1. the research of extractive of Zi-Jin-Sha, the effect of bisabolangelone treatment dysmenorrhea
The impact that 1.1 extractive of Zi-Jin-Sha, bisabolangelone shrink isolated uterine
Continuous three days subcutaneous injection estradiol benzoates (1mg/kg) before non-pregnant mouse experiment, make animal be in rutting period, to improve the sensitivity of uterine smooth muscle to medicine.Cervical dislocation puts to death mice, uterus, rapid clip side (about 2cm is long), be placed in the glass dish filling Rockwell liquid immediately, the connective tissue that careful removing is residual and fat, two ends are pricked with toe-in respectively, one end is fixed in isolated smooth muscle bath, and the other end is connected with tonotransducer.Add 16ml Rockwell liquid in groove, pass into 95%O continuously
2add 5%CO
2mist, constant temperature (37 ± 0.5) DEG C, load 1g.After the easypro contracting in uterus is stable, record one section of normal contraction activity curve.
(1) extractive of Zi-Jin-Sha, the bisabolangelone impact that causes Mouse Uterus to shrink on oxytocin
After specimen balance, in bath, add oxytocin makes final concentration be 0.01U/ml, the shrinkage curve of record one section of isolated uterine in contrast, after changing clothes nutritional solution in bath 3 times, add mutually commensurability oxytocin, after curve shrinkage is highly stable, add different extractive of Zi-Jin-Sha makes final concentration be 1.6 μ g/ml, 3.2 μ g/ml, the final concentration of bisabolangelone is 0.8 μ g/m, 1.2 μ g/ml, 1.6 μ g/ml, record each cross-talk palace shrinkage curve, statistics contraction frequency, mean tension, average amplitude, the results are shown in Table 1.
The impact that table 1 extractive of Zi-Jin-Sha, bisabolangelone cause Mouse Uterus to shrink on oxytocin
* p<0.05 is compared with matched group; * p<0.01 area=average amplitude × time
Above-mentioned result of the test shows, the Radix Pternopetali vulgaris petroleum ether part of variable concentrations, chloroform extract, ethyl acetate extract, and the bisabolangelone of variable concentrations all significantly reduces contraction frequency, mean tension and average amplitude that oxytocin causes.And along with the increase of Drug level, reduce degree and strengthen.
(2) extractive of Zi-Jin-Sha, bisabolangelone are to CaCl
2the uterotonic impact caused
After specimen balances, first rinse 3 times with without calcium High potassium solution, then in without calcium High potassium solution depolarization 30min, add CaCl
2final concentration is made to be 125 μ g/ml, record CaCl
2the shrinkage curve in the uterus caused in contrast.After changing clothes 3 times without calcium High potassium solution, first add different extractive of Zi-Jin-Sha and make final concentration be 1.6 μ g/ml, 3.2 μ g/ml, the final concentration of bisabolangelone is 0.8 μ g/ml, 1.2 μ g/ml, 1.6 μ g/ml, then adds mutually commensurability CaCl
2, record shrinkage curve change before and after comparing, the results are shown in Table 2.
The impact that table 2 extractive of Zi-Jin-Sha, bisabolangelone cause Mouse Uterus to shrink on CaCl2
* p<0.05 is compared with matched group; * p<0.01 area=average amplitude × time
Above-mentioned result of the test shows, the Radix Pternopetali vulgaris petroleum ether part of variable concentrations, chloroform extract, ethyl acetate extract, and the bisabolangelone of variable concentrations all significantly reduces CaCl
2the contraction frequency caused, mean tension and average amplitude.And along with the increase of Drug level, reduce degree and strengthen.This effect and suppression CaCl
2overflow is relevant.
(3) extractive of Zi-Jin-Sha, bisabolangelone uterotonic impact that High K+ depolarization is caused
After specimen balances, add KCl and make final concentration be 7.5 × 10
3μ g/ml, record shrinkage curve in contrast, after changing clothes nutritional solution 3 times, first adding different extractive of Zi-Jin-Sha makes final concentration be 1.6 μ g/ml, 3.2 μ g/ml, the final concentration of bisabolangelone is 0.8 μ g/ml, 1.2 μ g/ml, 1.6 μ g/ml, add mutually commensurability KCl again and record shrinkage curve, the contraction change before and after comparing, the results are shown in Table 3.
The impact that table 3 extractive of Zi-Jin-Sha, bisabolangelone cause Mouse Uterus to shrink on High K+ depolarization
* p<0.05 is compared with matched group; * p<0.01 area=average amplitude × time
Above-mentioned result of the test shows, the Radix Pternopetali vulgaris petroleum ether part of variable concentrations, chloroform extract, ethyl acetate extract, and the bisabolangelone of variable concentrations all significantly reduces mean tension, average amplitude and tension force maximum that KCl causes.And along with the increase of Drug level, reduce degree and strengthen.This effect is relevant with the Ca2+ influx suppressing High K+ depolarization to cause.
1.2 extractive of Zi-Jin-Sha, bisabolangelone are on the impact of oxytocin induced mice pain
Get Kunming kind female mice, random packet after title quality status stamp, often organizes 10.Primary dysmenorrhea model is manufactured with estradiol benzoate and oxytocin.By 10mg/kg lumbar injection estradiol benzoate injection, continuous 5d, to increase the sensitivity of uterine smooth muscle to medicine.Gastric infusion simultaneously, 1h after last administration, lumbar injection oxytocin 30U/kg take writhing response as the index of uterus strong contraction, and writhing incidence rate and the number of times of mice in rear 30min injected in record, the results are shown in Table 4.
Table 4 extractive of Zi-Jin-Sha, bisabolangelone are on the impact of oxytocin induced mice pain writhing
Compare with model group, * P < 0.05; * p<0.01 area=average amplitude × time
Above-mentioned result of the test shows, the Radix Pternopetali vulgaris petroleum ether part of various dose, chloroform extract, and ethyl acetate extract, bisabolangelone make mouse writhing reaction times all obviously reduce.Show that it has the effect for the treatment of dysmenorrhea.
The impact that 1.3 extractive of Zi-Jin-Sha, bisabolangelone shrink by force rat Uterus in vivo caused by oxytocin
Laboratory animal SD rat, female, body weight 230 ~ 250g, is provided by SanXia University's animal experimental center:
(1) animal grouping: get healthy adult female sd inbred rats 36, adaptability is fed and observed 3d to confirm health.Be divided into 12 groups at random, often organize 3, i.e. matched group, bisabolangelone group, Radix Pternopetali vulgaris petroleum ether part group, ethyl acetate extract group, chloroform extract group, n-butanol portion group.
(2) animals administer and nursing: timing every day of each group gives gavage 2 times, bisabolangelone by 8,10,12mg/kg administration, Radix Pternopetali vulgaris petroleum ether part group, ethyl acetate extract group, chloroform extract group, n-butanol portion group by 10,14mg/kg administration, matched group gives the normal saline of same volume, continuous use 7d, 8d morning, row Uterus in vivo experiment half an hour after last medicine, and 3d starts subcutaneous injection diethylstilbestrol injection 2mg/ (kgd) before experiment, be used in conjunction 2d, to improve the sensitivity of uterine smooth muscle.
(3) rat Uterus in vivo modelling: often organize gavage 8d, with 20% urethane intraperitoneal injection of anesthesia (1.3g/kg) after weighing, rat dorsal position is fixed on Mus plate, open abdomen and find out side cornua uteri, ligation dialysis ovary, blunt separation surrounding tissue, uterus far-end is drawn in uterus heat-preservation cylinder bottom outlet, clamp gently with the frog heart clip being connected with cotton thread, connect tension pick-up, this side uterus position, in the position of rat abdomen, is transferred in the middle part of heat-preservation cylinder by fixing uterus heat-preservation cylinder.And the De Shi liquid of 37 DEG C ~ 38 DEG C is added by hole on heat-preservation cylinder, make whole uterus be immersed in De Shi liquid, irradiate insulation with operating lamp.Bio signal analytical system starts to record uterine activity curve, recording curve 20 ~ 30min after uterine contraction is steady.Until shrinkage curve steadily after intramuscular injection oxytocin (20U/kg) record the frequency of rat uterine contractility, mean tension and area, the results are shown in Table 5.
The impact that table 5 extractive of Zi-Jin-Sha, bisabolangelone cause rat Uterus in vivo to shrink by force on oxytocin
* p<0.05 is compared with matched group; * p<0.01 area=average amplitude × time
Above-mentioned result of the test shows, Radix Pternopetali vulgaris petroleum ether part, ethyl acetate extract, chloroform extract, and the bisabolangelone of variable concentrations all significantly reduces contraction frequency, mean tension and the average amplitude that the oxytocin of rat Uterus in vivo causes.
The observation of 1.4 Radix Pternopetali vulgaris ethanol extract treatment dysmenorrhea effects
Clinical data: this is organized 60 routine patients and is institute of traditional Chinese medicine of Yichang City out-patient, and wherein middle school student account for the overwhelming majority.Unmarried 49 examples, married 11 examples; The oldest person 38 years old, age reckling 14 years old; 11 years that the course of disease is long, the course of disease was short 7 months; This is organized 60 routine patients and is without organic disease.
The new fresh ginger of Therapeutic Method: 10g is squeezed the juice filtered through gauze, adds 40mg ethanol extract and mixes thoroughly, on coating gauze, is affixed on SHENQUE acupoint, fixing.Once a day.Treatment time is through about the first seven day, treats three menstrual period continuously, if invalid, stops treatment.
Treatment standard: it is recovery from illness that pain disappears without recidivist; Pain obviously alleviates or attack times is reduced to effectively; Treat three through after date, pain is invalid before treating.
Therapeutic outcome: this group 60 example, recovery from illness 33 example, accounts for 55%; Effective 25 examples, account for 41.7%; Invalid 2 example, account for 3.3%; Total effective rate 96.7%.
Experimental result display Radix Pternopetali vulgaris has significantly treatment dysmenorrhea effect, and ethanol extract extraction position PetroChina Company Limited. ether position, chloroform extract, ethyl acetate extract is effective, and bisabolangelone is principle active component.Radix Pternopetali vulgaris suppresses uterine contraction, has the effect of prevention and therapy dysmenorrhea.
Embodiment 2
Extractive of Zi-Jin-Sha, bisabolangelone are at treatment and the Imaging in Patients with Cerebral Ischemia Disease preventing the hypertension relevant to calcium antagonist, atherosclerosis, apoplexy to cause.
2.1 calcium antagonism
Uterine Smooth Cell is cultivated:
Mouse anesthesia is put to death, rapid taking-up uterus is put in pre-cooling Hanke, in s liquid (0-4 DEG C), rinse 2-3 time, surrounding tissue is removed clean with shears, perimetrium blade is wiped off gently, tiling is longitudinally cutd open in uterus, lining endothelium fixes one end with tweezers upward, endometrium and base portion is wiped off gently, inner membrance and substrate are removed clean uterine muscle strips and shreds to 1mm from bearing with the syringe needle (bending into 30 degree of angles) of disposable syringe as separator
3with collagenase and 0.25% pancreatin mixture slaking liquid, 37 DEG C of water-bath concussion digestion 1h of 3ml0.25%, serum DMEM-F12 culture fluid is had to stop digestion, for several times, suspension is drawn in the sieved filter of 200 order cell in piping and druming, then by 400 order cell sieves, collect filtrate 1000rpm10min centrifugal, basic culture solution washes 2 times, removes cell debris, is inoculated in 35mm culture dish and cultivates.Platform is expected and blue repelled test and look into cell survival rate and be greater than 90%, uses 20%FCSDMEM culture fluid, put into 37 DEG C, 95% humidity, 5%CO
2incubator in cultivate, changed a not good liquor every 3 days.
2.1.1 extractive of Zi-Jin-Sha, bisabolangelone are on the impact of KCl-induced Uterine Smooth Cell calcium ion concentration
By uterine cell at room temperature with after the Fluo-3AM dyeing 60min of 100mmol/L esterified form, wash away extracellular residual fluorescence stain by Hank immersion, under phase contrast microscope, select active good cell test.In 40 orifice plates, active good cell is divided into 12 groups at random: the basic, normal, high concentration group of matched group, bisabolangelone, and extractive of Zi-Jin-Sha is low, high concentration group, measures reflection Free Ca with LSM-510 laser confocal microscope
2+intracellular Ca2+ fluorescence intensity (the excitation wavelength 488nm of concentration, emission wavelength 505nm), after measuring the basic value of calcium fluorescence intensity, give Hank ' S liquid respectively, final concentration be 0.8 μ g/ml, 1.2 μ g/ml, 1.6 μ g/ml bisabolangelones, measure calcium fluorescence intensity again after 1.6 μ g/ml, 3.2 μ g/ml extractive of Zi-Jin-Sha process 20min, then add potassium chloride and scan a width record calcium peak fluorescence intensity every 10s simultaneously.Each orifice plate is random selected 10 cells under laser confocal microscope, and calcium fluorescence intensity in analysis of cells is averaged after record peak value, be the results are shown in Table 6.
The impact that table 6 extractive of Zi-Jin-Sha and bisabolangelone change KCl-induced Uterine Smooth Cell calcium ion peak fluorescence intensity
* p<0.05 is compared with matched group; * p<0.01
2.1.2 extractive of Zi-Jin-Sha, bisabolangelone bring out the impact of Uterine Smooth Cell calcium ion concentration on caffeine
The same 2.1.1 of experimental technique, replaces potassium chloride with caffeine (final concentration 20mmol/L), the results are shown in Table 7.
Table 7 bisabolangelone and Radix Pternopetali vulgaris different parts extract bring out the impact of Uterine Smooth Cell calcium ion peak fluorescence intensity change to caffeine
* p<0.05 is compared with matched group; * p<0.01
Above-mentioned result of the test shows, the bisabolangelone of variable concentrations, Radix Pternopetali vulgaris petroleum ether part, chloroform extract, ethyl acetate extract not only have blocking effect to the extrinsic AGB stars of potassium chloride induction, and also have certain blocking effect to calcium release in caffeine induction, prove that bisabolangelone and petroleum ether part, chloroform extract, ethyl acetate extract extract have calcium antagonism, effectively can treat the cerebral ischemia caused with prophylaxis of hypertension, atherosclerosis, apoplexy.
Claims (1)
1. an extractive of Zi-Jin-Sha is treated in preparation and prevents the novelty teabag in the medicine of dysmenorrhea.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310377422.6A CN103432181B (en) | 2013-08-27 | 2013-08-27 | Common pternopetalum root extract and new use thereof in pharmacy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310377422.6A CN103432181B (en) | 2013-08-27 | 2013-08-27 | Common pternopetalum root extract and new use thereof in pharmacy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103432181A CN103432181A (en) | 2013-12-11 |
| CN103432181B true CN103432181B (en) | 2015-07-15 |
Family
ID=49685966
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310377422.6A Active CN103432181B (en) | 2013-08-27 | 2013-08-27 | Common pternopetalum root extract and new use thereof in pharmacy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103432181B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103156893A (en) * | 2011-12-09 | 2013-06-19 | 中国人民解放军军事医学科学院毒物药物研究所 | Novel application for angelica polymorpha maxim and extract thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPS323902A0 (en) * | 2002-06-27 | 2002-07-18 | Chinese Medicines Scientific Consultants Pty Ltd | Compositions and methods for treating gynaecological disorders |
-
2013
- 2013-08-27 CN CN201310377422.6A patent/CN103432181B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103156893A (en) * | 2011-12-09 | 2013-06-19 | 中国人民解放军军事医学科学院毒物药物研究所 | Novel application for angelica polymorpha maxim and extract thereof |
Non-Patent Citations (1)
| Title |
|---|
| 土家族药紫金砂化学成分及质量标准研究;万威;《中国优秀硕士学位论文全文数据库 医药卫生科技辑》;20091115;E056-997 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103432181A (en) | 2013-12-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102670696B (en) | Eucommia ulmoids leaf extracts and preparation method and application thereof | |
| CN102973693B (en) | Pharmaceutical composition used for relaxing bowels to lose weight, and preparation method and application thereof | |
| CN106236801A (en) | Pseudo-ginseng activity medicine that super-micro wall-broken crushing technology is made and health product and preparation method thereof | |
| CN106937959A (en) | A kind of antitumor extra large Blatta seu periplaneta extract and its preparation technology and detection method and purposes | |
| CN102327309B (en) | Barberry bark extractive and use of barberry bark extractive and barberry bark | |
| CN102085223A (en) | Sweet osmanthus flower extractive as well as preparation method and application thereof to liver protection | |
| CN104622865A (en) | Application of ingenane diterpene compound in preparation of antitumor drug | |
| CN102526141B (en) | Pterocephalus hookeri heck total iridoid glycoside extract and preparation method and application thereof | |
| CN102430078A (en) | Medicine for treating acute gouty arthritis and preparation method thereof | |
| CN102755343A (en) | Application of daucosterol in preparing medicines for promoting proliferation of neural stem cells | |
| CN107648297A (en) | A kind of fulvoushair honeysuckle flower extract, the preparation containing the extract and the application in field of medicaments | |
| CN103432181B (en) | Common pternopetalum root extract and new use thereof in pharmacy | |
| CN102058679B (en) | Medicine or health-care food composition for treating atherosclerosis | |
| CN105497098B (en) | Monimopetalum chinense extract with anti-inflammatory activity, preparation method and applications | |
| CN101574359B (en) | Pharmaceutical composition for treating cardiovascular and cerebrovascular disease | |
| CN101380359B (en) | Bone-building capsules and preparation method thereof | |
| CN102078395B (en) | Extract for treating rheumatoid arthritis | |
| CN100408034C (en) | Traditional Chinese medicine preparation for treating leukoderma, psoriasis and its preparation method | |
| CN103211833A (en) | Application of icariin in preparation of medicaments for treating gout | |
| CN109419823A (en) | Chinese yew extract and its application in preparation treatment rheumatoid arthritis external drug | |
| CN103239494B (en) | Nasal spray composition for treating migraine and preparation method thereof | |
| CN103393708A (en) | Ginsenoside-ophiopogonin D composition for treating cardiovascular and cerebrovascular diseases | |
| CN101983637B (en) | Radix trichosanthis saponin and application of radix trichosanthis saponin in preparing medicine for treating ischemic cerebrovascular diseases | |
| CN101548995A (en) | Application of Viscum album extract as NF-kB inhibitor | |
| CN102145075B (en) | Pharmaceutical composition for regulating liver and supplementing kidney, eliminating blood stasis and removing spot and preparation method of pharmaceutical composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |