CN103421691A - Glass chip for cultivating single cell array based on microfluidic patterning technology and preparation method thereof - Google Patents

Abstract

The invention discloses a glass chip for cultivating a single cell array based on microfluidic patterning technology, and a preparation method thereof, and belongs to the field of biological micro electro mechanical systems. The glass chip adopts the structure that a layer of PHEMA hydrogel pattern 2 is arranged on a glass base 4, and divides the glass base 4 into a plurality of rectangular cell growth zones 1 to generate the single cell array. The preparation process is that graphical PHEMA processing is performed on the surface of a glass sheet through a PDMS elastic stamp. The glass chip has the benefits that the stable surface chemical modification is adopted, so that hydrogel dressings capable of resisting cell adhesion is arranged on the surface of the glass in a graphical manner to form the cell graphical surface containing cell adhesion and resisting cell adhesion so as to cultivate the single cell array; the preparation process is simple and easy to operate; when the chip is applied to cell culture, the single cell growth array is obtained, and a new tool for studying basic cell biology is provided.

Description

A kind of unicellular array based on microfluid composition technology is cultivated glass-chip and preparation method thereof

Technical field

The present invention relates to a kind of unicellular array of realizing based on microfluid composition technology (microfluidic patterning, uFLP) and cultivate glass-chip structure and preparation method, belong to biological MEMS (micro electro mechanical system) (Bio-MEMS) field.

Background technology

Cell cultures is one of most important basic science in field such as biology, medical science, new drug development.Between in the past more than 200 year, cell cultures has been made huge contribution at aspects such as vaccine research and development, genetic analysis, biological studies.Along with the development of modern biochemistry, molecular biology, molecular genetics and modern medicine, cell cultures has also fully showed in many Application Areass the development potentiality that it is huge.The training method of conventional attached cell is a lot, take the cell colony cultivation as characteristics, as Tissue Culture Flask, Tissue Culture Dish, cell porous culture plate etc.Yet above-mentioned cell cultures mode all has following shortcoming: one, can not observe the immediate reaction of single or several cells to cell micro-environment; Under the condition of two, cultivating at cell colony, analyzing the result obtained is a kind of average index, causes some faint but very important reactions of iuntercellular to be covered; Three, can not be integrated into novel micro-analysis platform, realize the functions such as long-term detection for cell response, analysis.

In recent years, because Micrometer-Nanometer Processing Technology can obtain the characteristic dimension close with biomacromolecule, increasingly extensive in biological study and biomedical sector application.Simultaneously, Micrometer-Nanometer Processing Technology also provides the new approach that realizes for single cell culture.Realize the existing relevant exploratory development of single celled cultivation based on Micrometer-Nanometer Processing Technology, mainly contain following several method: one, surface chemical modification is carried out in the cell cultures substrate; Two, realize physical barrier in the cell cultures substrate; Three, microflow control technique.Wherein, to build unicellular figure be the more class methods of research to surface chemical modification.In conjunction with different biomolecules and characteristic group, as specific proteins, the short adhesion factor of cell, polysaccharide, alkanethiol, SAMs, polyelectrolyte etc., realize the graphical of biomolecules and characteristic group on surface, induce and produce unicellular figure.

Microfluid composition technology is a kind of important technology of realizing the cell pattern based on surface chemical modification: the elastomeric stamp of making is fitted in to substrate surface, by from airtight effect, forming the microchannel network, material for surface chemical modification forms graphical thin layer by the microchannel network at substrate surface, and postvaccinal cell is grown in the zone limited.Microfluid composition technology has that the course of processing is simple, the chemically modified density of material is convenient to the advantages such as control, but it is mainly used in forming the cell figure of continuous distribution, has limited the further application of this technology in the single cell culture field.People (the Sinclair J such as Sinclair J, Salem A K.Rapid localized cell trapping on biodegradable polymers using cell surface derivatization and microfluidic networking[J] .Biomaterials, 2006, 27 (9): 2090-2094.) utilize microfluid composition technology at Biodegradable material poly(lactic acid)-PEG surface graft avidin, form the patterned surface of poly(lactic acid)-PEG-avidin, the banded zone that is grown in avidin of cell selective, formed ribbon cell figure.In microfluid composition technology, microchannel commonly used network is straight channel, make and promote the chemically modified material of cell adhesion to form stripe-like at substrate surface, be difficult to control number and the position of cell on ribbon decorative material surface, can not realize unicellular array cultivation.

Summary of the invention

In prior art, there are the following problems: based on microfluid composition technology cell growth, substrate is promoted the chemically modified of cell adhesion, what obtain is that the ribbon identical with microchannel network plane structure modified figure, can not form closed region, therefore, the finishing that the method obtains can not restrictive cell growth position and shape, can not obtain unicellular array.

Emphasis of the present invention is: novel microchannel network plane structure, the decorative material sticked in conjunction with anti-cell, can obtain the growth position of restriction individual cells and the cellular growth area of shape by microfluid composition technology.

The objective of the invention is: proposed a kind ofly based on microfluid composition technology, can realize that unicellular array cultured cells array cultivates glass-chip structure and preparation method.

Technical scheme of the present invention is:

A kind ofly realize that unicellular array cultivates the glass-chip structure:

One deck PHEMA(poly (2-hydroxyethyl methacrylate) is arranged on substrate of glass 4, Poly-HEMA, polymethyl acrylic acid dihydroxy ethyl ester) hydrogel figure 2.PHEMA hydrogel figure 2 is divided into a plurality of rectangle cellular growth area 1 by substrate of glass 4 surfaces, for generation of unicellular array.The concrete structure size is as follows: the size dimension of rectangle cellular growth area 1 is respectively a, b,

Transverse pitch between adjacent two cellular growth area 1 medullary rays is w1,

Longitudinal pitch between adjacent two cellular growth area 1 medullary rays is w2,

The live width of the hydrogel figure projection of PHEMA hydrogel figure 2 is c, Be highly e,

The line thickness of hydrogel is d,

Article two, between adjacent PHEMA hydrogel, the narrowest distance is h,

Wherein

For treating the mean diameter of culturing cell 3.

A kind of cellular array cultivation glass-chip preparation method who realizes single cell culture:

Step 1: prepare clean sheet glass.After cleaning, drying for standby;

Step 2: the PHEMA ethanolic soln of preparing suitable concn;

Step 3: the method by the impression complex is made the PDMS elastomeric stamp, on described PDMS elastomeric stamp, has the microchannel network, and the two dimensional structure of described microchannel network is consistent with the PHEMA hydrogel figure 2 on prepared glass-chip;

Step 4: at the graphical PHEMA of glass sheet surface: by PDMS elastomeric stamp and glass reversible keying, add the PHEMA ethanolic soln in ingress, PDMS elastomeric stamp microchannel, complete the filling of solution in microchannel, PDMS elastomeric stamp after bonding and glass are placed in loft drier and heat, until ethanol volatilizees fully; Remove the PDMS elastomeric stamp, at glass surface, obtain the PHEMA dust figure;

Step 5: the sheet glass that surface is had to graphical PHEMA powder is soaked in pure water, obtains having the glass surface of PHEMA hydrogel figure, and the unicellular array of described single cell culture is cultivated glass-chip.

The invention has the beneficial effects as follows: broken through the existing restriction that can not realize single cell culture based on microfluid composition technology, proposed a kind of unicellular array for single cell culture and cultivated glass-chip structure and preparation method.Adopt stable surface chemical modification, the hydrogel sticked at the graphical anti-cell of glass surface, form and to comprise the patterned surface that cell adhesion and anti-cell stick, and for unicellular array, cultivates.

It is simple to operation that the unicellular array that the present invention proposes is cultivated the chip preparation process, usings glass as base material, do not need complicated surface treatment process; The graphic structure designed in chip is simple, and the processing of silicon masterplate can complete by ripe micro fabrication, and parameter is convenient to control, and the PDMS seal can obtain by repeat replication silicon template, has higher economy.This chip application, in cell cultures, can be obtained to unicellular growth array, will provide a kind of novel instrument for basic RESEARCH ON CELL-BIOLOGY.

The accompanying drawing explanation

Fig. 1 is the chip design sketch that unicellular array that the present invention proposes is cultivated glass-chip

Fig. 2 is the PDMS seal design sketch that unicellular array that the present invention proposes is cultivated glass-chip

Fig. 3 is that the unicellular array that the present invention proposes is cultivated the glass-chip structural representation

Fig. 4 is the silicon template machining process route used in embodiment chips preparation process

Fig. 5 is the manufacture craft route that in embodiment, unicellular array is cultivated glass-chip

In figure: 1. cellular growth area, 2.PHEMA hydrogel figure, 3. cell, 4. substrate of glass

Embodiment

Embodiment 1:

Chip in the present embodiment carries out cells contacting connection research for cultivating single scleroblast MC3T3-E1.The MC3T3-E1 mean diameter Be 20 μ m.

Consult Fig. 1～Fig. 3.In the present embodiment, unicellular array is cultivated to glass-chip for the MC3T3-E1 cell cultures.Wherein, the scantlings of the structure of unicellular array cultivation glass-chip is as follows: the size a=b=50 μ m of cellular growth area 1; Transverse pitch w1=70 μ m between adjacent two cellular growth area 1 medullary rays; Longitudinal pitch w2=80 μ m between adjacent two cellular growth area 1 medullary rays; The live width d=20 μ m of PHEMA hydrogel figure 2, hydrogel figure bossing size c=e=20 μ m; Article two, the narrowest distance h between adjacent PHEMA hydrogel=20 μ m.

Realize unicellular array cultivation glass-chip preparation method based on microfluid composition technology in the present embodiment, adopt MEMS technology and microfluid composition technology to complete, specifically comprise the steps:

Step 1: prepare clean sheet glass.Purging method: sheet glass cleans up with liquid detergent, dries; Sheet glass is longer than 12h in the potassium bichromate solution soak time; Large water gaging rinses, drying for standby.

Step 2: the PHEMA ethanolic soln that preparation concentration is 50%.According to mass concentration (being solute quality/solvent volume), 50% prepared, a certain amount of PHEMA white powder of weighing and analytically pure ethanol, mixed, two days later standing, obtains the PHEMA ethanolic soln of clear.

Step 3: the method by the impression complex is made the PDMS elastomeric stamp:

At first prepare to mix PDMS.According to mass ratio 10:1 configuration PDMS prepolymer and linking agent, mix rear vacuumize degassing 4～5 times, in mixing solutions visual inspection less than bubble till.

Above-mentioned ready PDMS slowly is poured on the silicon template processed, if again occur bubble in PDMS liquid, repeats once degassed; Silicon template after cast PDMS is placed in to 80 ℃ of 1h of vacuum drying oven heating, makes PDMS that crosslinking reaction occur, solidify.Peel off PDMS from the silicon template, obtain having the PDMS elastomeric stamp of microchannel network, the two dimensional structure of described microchannel network is consistent with the PHEMA hydrogel figure 2 on prepared glass-chip;

Step 4: at the graphical PHEMA of glass surface.Based on the PDMS material from closed nature, make PDMS elastomeric stamp and glass reversible keying, use liquid-transfering gun or glue head straw to add a certain amount of PHEMA ethanolic soln in the ingress, microchannel, utilize capillary force to drive the solution automatic filling, whole process completes needs several minutes; After solution has been filled, PDMS elastomeric stamp and glass are placed on to 100 ℃ of 15min of heating in loft drier, until ethanol volatilizees fully; Remove the PDMS elastomeric stamp, at glass surface, obtain the PHEMA dust figure.

Step 5: obtain unicellular array and cultivate glass-chip.The sheet glass that surface is had to a graphical PHEMA powder is soaked in pure water more than 12h, obtains having the glass surface of PHEMA hydrogel figure, obtains the described unicellular array of realizing based on microfluid composition technology and cultivates glass-chip.

Embodiment 2:

Chip in the present embodiment carries out the growth characteristics research of cell for cultivator mammary epithelial cell HMEC.The HMEC mean diameter Be 45 μ m.

Consult Fig. 1～Fig. 3.In the present embodiment, unicellular array is cultivated to glass-chip for the HMEC cell cultures.Wherein, the scantlings of the structure of unicellular array cultivation glass-chip is as follows: the size a=b=80 μ m of cellular growth area 1; Transverse pitch w1=120 μ m between adjacent two cellular growth area 1 medullary rays; Longitudinal pitch w2=140 μ m between adjacent two cellular growth area 1 medullary rays; The live width d=40 μ m of PHEMA hydrogel figure, the size c=e=40 μ m of hydrogel figure bossing; Article two, the narrowest distance h between adjacent PHEMA hydrogel=20 μ m.

Realize unicellular array cultivation glass-chip preparation method based on microfluid composition technology in the present embodiment, adopt MEMS technology and microfluid composition technology to complete, specifically comprise the steps:

Step 1: prepare clean sheet glass.Purging method: sheet glass cleans up with liquid detergent, dries; Sheet glass soaks duration at potassium bichromate solution and is greater than 12h; Large water gaging rinses, drying for standby.

Step 2: the PHEMA ethanolic soln that preparation concentration is 50%.According to mass/volume ratio=50%, a certain amount of PHEMA white crystal of weighing and analytically pure ethanol, mixed, two days later standing, obtains the PHEMA ethanolic soln of clear.

Step 3: the method by the impression complex is made the PDMS elastomeric stamp:

At first prepare to mix PDMS.According to mass ratio 10:1 configuration PDMS prepolymer and linking agent, mix rear vacuumize degassing 4～5 times, in mixing solutions visual inspection less than bubble till.

Above-mentioned ready PDMS slowly is poured on the silicon template processed, if again occur bubble in PDMS liquid, repeats once degassed; Silicon template after cast PDMS is placed in to 80 ℃ of 1h of vacuum drying oven heating, makes PDMS that crosslinking reaction occur, solidify.Peel off PDMS from the silicon template, obtain having the PDMS elastomeric stamp of microchannel network, the two dimensional structure of described microchannel network is consistent with the PHEMA hydrogel figure 2 on prepared glass-chip;

Step 4: at the graphical PHEMA of glass surface.Based on the PDMS material from closed nature, make PDMS elastomeric stamp and glass reversible keying, use liquid-transfering gun or glue head straw to add the PHEMA ethanolic soln in the ingress, microchannel, utilize capillary force to drive the solution automatic filling, whole process completes needs several minutes; After solution has been filled, PDMS elastomeric stamp and glass are placed on to 100 ℃ of 15min of heating in loft drier, until ethanol volatilizees fully; Remove the PDMS elastomeric stamp, at glass surface, obtain the PHEMA dust figure.

Step 5: obtain unicellular array and cultivate glass-chip.The sheet glass that surface is had to a graphical PHEMA powder is soaked in pure water more than 12h, obtains having the glass surface of PHEMA hydrogel figure, obtains the described unicellular array of realizing based on microfluid composition technology and cultivates glass-chip.

Claims (2)

1. can realize unicellular array cultivation glass-chip structure for one kind, it is characterized in that, be included in one deck PHEMA hydrogel figure (2) on substrate of glass (4), PHEMA hydrogel figure (2) is divided into a plurality of rectangle cellular growth area (1) by substrate of glass (4) surface, for generation of unicellular array; The size dimension of described rectangle cellular growth area (1) is respectively a, b,

Transverse pitch between adjacent two cellular growth area (1) medullary ray is w1,

Longitudinal pitch between adjacent two cellular growth area (1) medullary ray is w2,

The live width of the hydrogel figure projection of PHEMA hydrogel figure (2) is c,

Be highly e,

The line thickness of hydrogel is d,

Article two, between adjacent PHEMA hydrogel, the narrowest distance is h,

Wherein For treating the mean diameter of culturing cell (3).

2. the preparation method that can realize as claimed in claim 1 the cellular array cultivation glass-chip of single cell culture, is characterized in that, comprises the steps:

Step 1: prepare clean sheet glass.After cleaning, drying for standby;

Step 2: the PHEMA ethanolic soln of preparing suitable concn;

Step 3: the method by the impression complex is made the PDMS elastomeric stamp, on described PDMS elastomeric stamp, has the microchannel network, and the two dimensional structure of described microchannel network is consistent with the PHEMA hydrogel figure 2 on prepared glass-chip;

Step 4: at the graphical PHEMA of glass sheet surface: by PDMS elastomeric stamp and glass reversible keying, add the PHEMA ethanolic soln in ingress, PDMS elastomeric stamp microchannel, complete the filling of solution in microchannel, PDMS elastomeric stamp after bonding and glass are placed in loft drier and heat, until ethanol volatilizees fully; Remove the PDMS elastomeric stamp, at glass surface, obtain the PHEMA dust figure;

Step 5: the sheet glass that surface is had to graphical PHEMA powder is soaked in pure water, obtains having the glass surface of PHEMA hydrogel figure, and the unicellular array of described single cell culture is cultivated glass-chip.

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