CN103417668A

CN103417668A - Application of sorbus sibirica fruit extract to preparation of drugs or health care products with HeLa cell resistance

Info

Publication number: CN103417668A
Application number: CN2013103212337A
Authority: CN
Inventors: 魏杰; 高军; 石佳; 周志权
Original assignee: Liaoning University
Current assignee: Liaoning University
Priority date: 2013-07-29
Filing date: 2013-07-29
Publication date: 2013-12-04

Abstract

The invention relates to application of a sorbus sibirica fruit extract to preparation of drugs or health care products with HeLa cell resistance. Aiming at development and utilization of novel drugs and health care products capable of efficiently inhibiting HeLa cells, the application of the sorbus sibirica fruit extract to preparation of the drugs or health care products with HeLa cell resistance is provided. The sorbus sibirica fruit extract applied in the invention has the advantages as follows: the research on the inhibition ratio of the extract on the HeLa cells shows that the sorbus sibirica fruit extract has strong capability of inhibiting growth of the HeLa cells, and when the concentration of the extract is 200 microgram/mL and the culture time is 72 hours, the inhibition ratio achieves 50%. Therefore, the sorbus sibirica fruit extract has great HeLa cell resistance, and is a good choice for developing novel efficient natural anti-cancer drugs.

Description

The Sorbus sibirica fruit extract is in the medicine of the anti-HeLa cancerous cell of preparation or the application in health product

Technical field

The invention belongs to the purposes field of medicine, relate to particularly the Sorbus sibirica fruit extract and there is the medicine of anti-HeLa cervical cancer cell effect or the application in health product in preparation.

Background technology

Cancer is the highest disease of developed country's mortality rate, also becomes gradually the second largest lethal disease that is only second to cardiovascular disease in developing country.Cervical cancer is one of modal malignant tumor in whole world women, is in the first place of gynecologic malignant tumor in China always, is also that women's cancer mortality is one of higher.Natural product is important health food resource and the important sources of drug development, how natural product is applied to cancer drug exploitation and clinical treatment aspect, is the constantly problem of exploration of those skilled in the art.

Sorbus sibirica is Rosaceae (Rosaceae) Fructus Sorbi Pohuashanensis, belongs to dungarunga, in China, only is distributed in upstream, Burqin river, likes growing under PiceameyeriRehd. Et Wils. and fir mixed forest, and its tree-like grace, figure hold directly, and leaf is large-scale winglike compound leaf, is rich in ornamental value.Spring, snow-white flower was completely set fragrance, and the scarlet wish of mellow fruit in autumn is dripped.

The Sorbus sibirica fruit extract does not also appear in the newspapers in the application facet that suppresses the HeLa cervical cancer cell at present.

Summary of the invention

The present invention seeks the medicine of new and effective inhibition HeLa cancerous cell or the exploitation of health product are purpose, has proposed the Sorbus sibirica fruit extract and has had the medicine of anti-HeLa cancerous cell effect or the application in health product in preparation.

The Sorbus sibirica fruit extract that the present invention obtains, research by extract to HeLa inhibition of cancer cell rate shows: the Sorbus sibirica fruit extract has the ability of very strong inhibition HeLa growth of cancer cells, when extract concentrations is 200 μ g/mL, while cultivating 72h, suppression ratio just reaches 50%.Therefore, the anti-HeLa cancerous cell of Sorbus sibirica fruit extract ability is strong, is good a selection of the efficient natural anti-cancer drugs of development of new.

The accompanying drawing explanation

Fig. 1 is the inhibitory action of Sorbus sibirica fruit extract to the HeLa cell.

The specific embodiment

Embodiment 1

(1) preparation of Sorbus sibirica fruit extract: take Sorbus sibirica fruit 5 g, ethanol with 60% soaks 1 h at 60-70 ℃, put into the ultrasonic 30min of ultrasonic cleaner, continuous 2 times, use again the Rotary Evaporators concentrated extracting solution, through vacuum lyophilization, obtain Sorbus sibirica fruit extract dry powder.

(2) effect experiment

1, sample preparation

The preparation of 1640 complete mediums: hyclone is put into 55 ℃ of water-bath 30min deactivation, percentage ratio by volume, get 10% hyclone and 90% RPMI-1640 mix homogeneously, then add two anti-(penicillin and streptomycin mix by weight 1:1), addition is 100u/ml, mix homogeneously, make 1640 complete mediums.

The Sorbus sibirica fruit extract dry powder 0.01g obtained is dissolved in to 20% alcoholic solution 1ml, filtration sterilization, being mixed with concentration with 1640 complete mediums is the mother solution that contains Sorbus sibirica fruit extract 1mg/mL, is stored in-4 ℃ of Refrigerator stores.

Get the mother solution that contains Sorbus sibirica fruit extract 1mg/mL, be mixed with respectively with 1640 complete mediums the extract solution that concentration is 60 μ g/mL, 80 μ g/mL, 100 μ g/mL, 150 μ g/mL, 200 μ g/mL, 250 μ g/mL standby.For following effect experiment.

2, test

Taking the logarithm, (cell density is about 3 * 10 to trophophase HeLa cell ⁷) being inoculated in 96 orifice plates, 1000 cells are inoculated in every hole; It is divided into to following seven groups:

Matched group: 1640 complete mediums of 100 μ L;

Experimental group: standby extract solution (60 μ g/mL, 80 μ g/mL, 100 μ g/mL, 150 μ g/mL, 200 μ g/mL, 250 μ g/mL) adds respectively 100 μ L, every group of 6 parallel holes;

In 37 ℃ of incubators, cultivate, respectively at 24h, 48h adds 5mg/mLMTT solution 10 μ L in each hole after 72h, and after continuing to cultivate 4h, spend the night in 37 ℃ of incubators after adding 100 μ LDMSO dissolving crystallized in every hole; Measure the absorbance of each hole at the 490nm place with enzyme-linked immunosorbent assay instrument, be calculated as follows cell inhibitory rate, result as shown in Figure 1.

Suppression ratio (%)=(1 one A _Experiment/ A _Contrast) X100%

As shown in Figure 1, the Sorbus sibirica fruit extract has the ability of very strong inhibition HeLa Growth of Cells, and when extract concentrations is 100 μ g/mL, while cultivating 72h, suppression ratio just reaches 45%; When extract concentrations is 200 μ g/mL, while cultivating 72h, suppression ratio just reaches 50%.Therefore, the anti-HeLa cell ability of Sorbus sibirica fruit extract is strong, is development of new good a selection of natural anti-HeLa cervical cancer cell efficiently.

Claims

1. the Sorbus sibirica fruit extract is in the medicine of the anti-HeLa cancerous cell of preparation or the application in health product.

2. application as claimed in claim 1, the preparation method that it is characterized in that described Sorbus sibirica fruit extract is as follows: get the Sorbus sibirica fruit, ethanol with 60% soaks 1 h at 60-70 ℃, puts into the ultrasonic 30min of ultrasonic cleaner, then uses the Rotary Evaporators concentrated extracting solution.