CN103417634B - A kind of Ramulus Mori active site and its preparation method and application - Google Patents
A kind of Ramulus Mori active site and its preparation method and application Download PDFInfo
- Publication number
- CN103417634B CN103417634B CN201310297505.4A CN201310297505A CN103417634B CN 103417634 B CN103417634 B CN 103417634B CN 201310297505 A CN201310297505 A CN 201310297505A CN 103417634 B CN103417634 B CN 103417634B
- Authority
- CN
- China
- Prior art keywords
- ramulus mori
- active site
- preparation
- exchange resin
- mori active
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to the preparation field of traditional Chinese medicine active site, disclose a kind of Ramulus Mori active site and its preparation method and application.After the method comprises the steps: that S1. Ramulus Mori is pulverized, with the ethanol of 10 ~ 40 times 25% ~ 95%, heating and refluxing extraction 1 ~ 3 time under 25 ~ 100 DEG C of conditions, each extraction time is 60 ~ 300min; Extracting liquid filtering is concentrated to obtain stock solution; S2. by stock solution first after through storng-acid cation exchange resin and anion exchange resin enrichment, purification, obtain Ramulus Mori active site.Described preparation method is easy and simple to handle; Reproducible, in the Ramulus Mori active site prepared, the content of 1-DNJ (DNJ) can reach more than 30.0%.
Description
Technical field
The present invention relates to the preparation field of traditional Chinese medicine active site, more specifically, relate to a kind of Ramulus Mori active site and
Its preparation method and application.
Background technology
Diabetes are inherited genetic factors and the chronic generalized metabolic disease of the one caused by environmental factors long term, often produce the complication of the aspects such as cardiovascular, nerve, eye.Danting medalist Mr professor Garry proposes, and lipoidosis is that the protopathy reason of diabetes and complication thereof changes, and in type 2 diabetes mellitus, the root of carbohydrate metabolism disturbance is Anomalous lipid metablism, is even called by type 2 diabetes mellitus " glycolipid is sick ".
Ramulus Mori be moraceae plants Mulberry (
morus albal
) twig, its medicinal history is long.Contained by Ramulus Mori, chemical composition kind is more, mainly contains polysaccharide, flavonoid, Coumarins, alkaloid etc., in addition containing volatile oil, aminoacid, organic acid and various vitamin etc." Chinese Pharmacopoeia " records it for wind-damp dispelling, the common medicine in sharp joint, is used for the treatment of shoulder arm, joint aches is numb.Being applied to the various diseases such as arthralgia, numb hand and foot, rheumatic arthralgia, paralysis clinical more.
Modern pharmacology proves, Ramulus Mori has blood sugar lowering, the effect such as blood fat reducing and antiviral.Clinical have Ramulus Mori Chinese patent medicine particle to be used for the treatment of diabetes especially.Ye Fei etc. study discovery, and to alloxan hyperglycemic rat continuous oral Ramulus Mori extract, the indexs such as hyperglycemic rat empty stomach and non-fasting serum glucose all obviously reduce, blood fat is adjusted, and diabetic nephropathy improves.Kimura etc. observed its hypoglycemic activity to the diabetic mice that STZ causes.Chen Youzhu etc. find that Morus alba branch and Morus multicaulis branch ethanol extraction all significantly can reduce the fasting glucose of the diabetic mice that alloxan causes.But above-mentioned application does not provide a complete Ramulus Mori hypoglycemic active site concept and definite data, and the assay of the hypoglycemic active site of Ramulus Mori and quality control aspect wretched insufficiency.Therefore, current Ramulus Mori preparation is generally crude extract, all serious test to its curative effect and quality control, if so can obtain a good active site and provide a preliminary method of quality control by for Ramulus Mori preparation or functional health care food exploitation and use bring great opportunity.
Summary of the invention
Technical problem to be solved by this invention is, in order to overcome the above-mentioned deficiency of Ramulus Mori extract in prior art, provides a kind of preparation method of Ramulus Mori active site.
Another technical problem to be solved by this invention is, provides a kind of Ramulus Mori active site of being prepared by above-mentioned preparation method and application thereof.
Above-mentioned technical problem to be solved by this invention is achieved by the following technical programs:
A preparation method for Ramulus Mori active site, is characterized in that, comprises the steps:
S1. after Ramulus Mori is pulverized, with the ethanol of 10 ~ 20 times 50% ~ 95%, reflux under 25 ~ 100 DEG C of conditions
Extract 1 ~ 3 time, each extraction time is 60 ~ 300min; Extracting liquid filtering is concentrated into and obtains stock solution without alcohol taste;
S2. by stock solution first after through storng-acid cation exchange resin and anion exchange resin enrichment, purification, obtain Ramulus Mori active site.
The ethanol of described 25% ~ 95% refers to that volume fraction is the ethanol water of 25% ~ 95%.
The ethanol of described use 10 ~ 20 times 50% ~ 95% refers to the ethanol of 50% ~ 95% of 1 kg Ramulus Mori, 10 ~ 20 l, or the 1g Ramulus Mori ethanol of 50% ~ 95% of 10 ~ 20 ml.
As a kind of preferred version, by the ethanol of 10 ~ 20 times 50% ~ 80% heating and refluxing extraction under 60 ~ 80 DEG C of conditions in S1, each extraction time is 10min.
As one most preferably scheme, by the ethanol of 20 times 54% heating and refluxing extraction under 74 DEG C of conditions in S1, each extraction time is 120min.
As a kind of preferred version, described extraction time is 2 times
As a kind of preferred version, the operating procedure of S2 middle strong acidity cation exchange resin and anion exchange resin enrichment, purification is: stock solution adsorbed by storng-acid cation exchange resin, then use the ammonia of 0.2 ~ 1N to carry out eluting, collect eluent evaporate to dryness, obtain sample 1; Again by sample 1 water dissolution, slow transit through strong-base anion-exchange resin, water elution, volatilize eluent and obtain sample 2, be i.e. Ramulus Mori active site.
As a kind of preferred version, described ammonia concn is 0.5N.
As a kind of preferred version, the described storng-acid cation exchange resin in described preparation side is the strong acid type resin of crosslinked polystyrene system or has the resin of same effect; Described strong-base anion-exchange resin is the strong base resin of crosslinked polystyrene system or has the resin of same effect.
As one most preferably scheme, described cation exchange resin is the strong acid type resin of crosslinked polystyrene system, and its model is 001 × 7; Anion exchange resin described in it is the strong base resin of crosslinked polystyrene system, and its model is 201 × 7.
The present invention also provides a kind of Ramulus Mori active site prepared by above-mentioned preparation method.
Described Ramulus Mori active site is mainly containing alkaloids composition, and the content of wherein said 1-DNJ (DNJ) can reach 31.0%.
Above-mentioned Ramulus Mori active site is preparing the application in Inhibiting α-glucosidase medicine.
Above-mentioned Ramulus Mori active site is in preparation treatment and prevent diabetes, application in the medicine of obesity, hyperlipidemia or viral infection.
As a kind of preferred version, described Ramulus Mori active site is combined with excipient substance and is prepared into liquid preparation or solid preparation.
As one most preferably scheme, described solid preparation is granule, tablet, capsule (containing soft capsule) or drop pill; Described liquid preparation is oral liquid or injecting fluid preparation.
The form of medication of above-mentioned preparation mainly comprises oral administration and parenterai administration, preferred oral administration.
The present invention's beneficial effect specific as follows:
(1) the present invention uses different extraction conditions, obtains optimum extraction conditions, creates the extraction process of more saving, and saves industrialization cost widely; Described extracting method is easy and simple to handle; Reproducible, resin etc. are reusable;
(2) the Ramulus Mori active site that obtains after treatment of the present invention, wherein the content of 1-DNJ (DNJ) can reach more than 30.0%.After described extraction, Ramulus Mori active site only accounts for about 8 ‰ of medical material content, is convenient to prepare Ramulus Mori blood sugar reducing preparation.
(3) the Ramulus Mori active site safety for preparing of the present invention is high.
Accompanying drawing explanation
Fig. 1 is the high-efficient liquid phase chromatogram after analyte derivative.
Fig. 2 is that Ramulus Mori active site and DNJ are to the inhibitory action of alpha-glucosidase.
Detailed description of the invention
Explain the present invention further below in conjunction with specific embodiment, but embodiment does not limit in any form to the present invention.
The preparation method of embodiment 1 Ramulus Mori active site
Get Ramulus Mori 100g, 4 parts, pulverize, sieve.Use pure water, 25% ethanol water, 50% ethanol water, 75% ethanol water boil respectively, extract 2 times, liquid-solid ratio 10 ml/g, each concentration level is parallel carries out 3 times, and crosses strong-acid ion exchange resin 732(1:20=m/m respectively), then carry out eluting with the ammonia of 0.N, collect eluent evaporate to dryness, water dissolution slow transits through strong-base anion-exchange resin 201 × 7 again, water elution, volatilizes eluent and obtains Ramulus Mori active site.The wherein maximum average out to 0.78g of Ramulus Mori active site amount that obtains of 50% ethanol-water extraction.
The preparation method of embodiment 2 Ramulus Mori active site
Get Ramulus Mori 5g, pulverize, sieve, by the ethanol of 10 ~ 20 times 50% ~ 80% reflux 2 times under 60 ~ 80 DEG C of conditions, each 60 ~ 180min, obtains stock solution by extracting liquid filtering concentration and recovery ethanol.Stock solution is adsorbed by storng-acid cation exchange resin, then carries out eluting with the ammonia of 0.5N, collect eluent evaporate to dryness, obtain sample 1.Again by sample 1 water dissolution, slow transit through strong-base anion-exchange resin, water elution, volatilize eluent and obtain sample 2. i.e. Ramulus Mori active site.The results are shown in Table 1.
The amount at what table 1 various extracting conditions obtained have hypoglycemic activity position
| Coding | Temperature DEG C | Time min | Determining alcohol % | Liquid-solid ratio ml/g | Total alkaloids (g) |
| 19 | 70 | 120 | 65 | 15 | 0.08 |
| 18 | 70 | 180 | 65 | 20 | 0.08 |
| 15 | 70 | 120 | 65 | 20 | 0.079 |
| 20 | 70 | 120 | 65 | 15 | 0.076 |
| 11 | 80 | 120 | 65 | 20 | 0.075 |
| 2 | 70 | 120 | 65 | 15 | 0.071 |
| 7 | 70 | 120 | 65 | 15 | 0.068 |
| 10 | 70 | 180 | 50 | 15 | 0.068 |
| 17 | 60 | 120 | 65 | 20 | 0.066 |
| 4 | 70 | 120 | 50 | 20 | 0.065 |
| 28 | 80 | 120 | 50 | 15 | 0.064 |
| 1 | 70 | 120 | 50 | 10 | 0.064 |
| 9 | 70 | 180 | 80 | 15 | 0.061 |
| 27 | 80 | 120 | 80 | 15 | 0.061 |
| 26 | 70 | 120 | 80 | 10 | 0.055 |
| 12 | 60 | 180 | 80 | 20 | 0.051 |
| 29 | 70 | 60 | 65 | 10 | 0.05 |
| 16 | 60 | 120 | 50 | 15 | 0.048 |
| 6 | 70 | 180 | 65 | 10 | 0.043 |
| 5 | 80 | 120 | 65 | 10 | 0.041 |
| 21 | 70 | 60 | 80 | 15 | 0.041 |
| 25 | 70 | 60 | 50 | 15 | 0.039 |
| 22 | 60 | 120 | 65 | 10 | 0.037 |
| 23 | 70 | 120 | 80 | 20 | 0.035 |
| 24 | 60 | 120 | 80 | 15 | 0.034 |
| 13 | 70 | 60 | 80 | 20 | 0.033 |
| 8 | 80 | 60 | 65 | 15 | 0.03 |
| 14 | 80 | 120 | 65 | 15 | 0.03 |
| 3 | 60 | 60 | 65 | 15 | 0.029 |
Designed by the response surface, show that Ramulus Mori active site optimum process condition is Extracting temperature 70 DEG C, extraction time 160min, concentration of alcohol 54%, liquid-solid ratio 20ml/g.
The content assaying method of embodiment 3 Ramulus Mori active site main constituent-DNJ
get above-mentioned sample solution, reference substance solution, blank solution 50 μ l respectively in the centrifuge tube of 1.5ml, add 0.4mol/L potassium borate buffer solution 200 μ l, add 5mmol/L FMOC-Cl (50% acetonitrile configuration) 250 μ l again, shake up, room temperature places 30 minutes, then adds 1mol/L glycine (Gly) 25 μ l stopped reaction, add the acetum 80 μ l that volume fraction is 1% again, add water and be settled to 1000 μ l, last solution organic membrane filter, obtains test fluid.Test fluid is tested through liquid chromatograph, and liquid phase chromatogram condition is: chromatographic column: Ultimate
tMxB-C18 chromatographic column (4.6 mm × 250 mm); Mobile phase: acetonitrile-0.1% acetic acid (38:62); Flow velocity: 1ml/min; Column temperature: room temperature; Wavelength: 254nm; Sample size: 20 μ l.
As can be seen from Figure 1, the DNJ in sample and adjacent component all obtain good baseline separation.The mensuration of the equal not interfering component of hydrolysising by-product FMOC-OH and Gly-FMOC of reagent.
The content of DNJ in the sample prepared under measuring optimum process condition by above-mentioned content assaying method, result is as follows:
The content of the DNJ in table 2 Ramulus Mori active site
| Sample | DNJ content |
| Stock solution | 0.23% |
| Sample 1 | 13.2% |
| Sample 2 | 31.0% |
Embodiment 4 Ramulus Mori active site is to the suppression of alpha-glucosidase activity
Get pH6.8 phosphate buffered solution 1.85 ml, add 0.1 Uml successively
-1alpha-glucosidase 50 μ L, Ramulus Mori active site solution (0,1,2,3,4,5,6,7 mgml of variable concentrations
-1) 50 μ L, 0.0116 molL
-1pNPG 50 μ L, by above-mentioned reaction system after 37 DEG C of waters bath with thermostatic control are incubated 30 min, adds 0.1 molL immediately
-1na
2cO
3solution 8 ml cessation reaction, each concentration is parallel does 3 parts, averages.Described Ramulus Mori active site prepares under optimum process condition in example 2.
The inhibitory action figure of Ramulus Mori active site to alpha-glucosidase is obtained, as shown in Fig. 2 according to above-mentioned method and computing formula.Can significantly find out from figure, Ramulus Mori active site has very strong inhibitory action to alpha-glucosidase.
Embodiment 5 Ramulus Mori active site is on the impact of normal mouse post-prandial glycemia
The normal mouse of body weight 22 ~ 25 g is divided into blank group, positive controls (acarbose), DNJ administration group and 3 administration groups at random by body weight, often organizes 10, three administration components.Before test, water is can't help in 16h fasting, get the Ramulus Mori active site of certain concentration, acarbose and DNJ respectively to feed to normal mouse filling, fill with simultaneously and feed starch (3 g/kg), docking blood sampling in 0 minute, 30 minutes, 60 minutes, 120 minutes upon administration, adopts blood glucose meter to measure mouse blood sugar value respectively.Normal mouse after the meal 30 min blood glucose values reaches summit, then falls after rise gradually, substantially returns to close to fasting level to blood glucose value during 120 min.Described Ramulus Mori active site prepares under optimum process condition in example 2.
Table 3. Ramulus Mori active site is on the impact of normal glucose tolerance in mice
With negative control group ratio, * p<0.05, * * p<0.01:(n >=8)
Result shows: Ramulus Mori active site and DNJ significantly can reduce normal mouse level of postprandial blood sugar in the level of 2.0-8.0 mg/kg, and has certain time delaying blood glucose and peak.Administration group is compared with matched group, and blood glucose value has significant difference (p<0.05).
Embodiment 6 Ramulus Mori active site is on the impact of diabetic mice post-prandial glycemia
Modling model: choose about 25 g male mice in kunming, after water is can't help in 18 h fasting, by 200 mg/kg body weight lumbar injection alloxan (2.5% normal saline solution), blood is got after 48 h, measure the blood glucose value after fasting 12, blood glucose value is decided to be diabetic rat model the mice of 11 more than mmol/L.Diabetic mice is divided into 4 groups at random, often organizes 8, be respectively diabetes model negative control group, positive controls, 2 experimental grouies.Within 0,30,60,120 minutes, measure mouse blood sugar value upon administration respectively, investigation Ramulus Mori active site and DNJ are on the impact of diabetic mice sugar drug resistance.Described Ramulus Mori active site prepares under optimum process condition in example 2.
Table 4. Ramulus Mori active site is on the impact of diabetic mice post-prandial glycemia
Compare with negative control, * p<0.05, * * p<0.01; (n=8)
Result shows: Ramulus Mori active site position and DNJ all can delay blood glucose and peak the time, also can reduce diabetic mice postprandial hyperglycemia level simultaneously, after administration 30min, 60min, administration group is compared with diabetes negative control group, and blood glucose value has significant difference (p 0.05).
Embodiment 7 acute toxicity testing
8 22 ~ 25g normal mouses, raise with standard feed, freely drink water.Ramulus Mori active site extract is made into 0.4 g/ml, fills with at twice and feed administration, each 0.2 ml/10g body weight, two minor ticks 5 hours.Total dosage is 8.0 g/kg.Continuous Observation one week.
As a result, Continuous Observation, after one week, has no animal dead and other poisoning symptoms.Dissect and check, also no abnormality seen.
Claims (7)
1. a preparation method for Ramulus Mori active site, is characterized in that, comprises the steps:
S1. after Ramulus Mori is pulverized, with the ethanol of 20 times 54%, heating and refluxing extraction 2 times under 70 DEG C of conditions, each extraction time is 160min; Extracting liquid filtering is concentrated to obtain stock solution;
S2. by stock solution first after through storng-acid cation exchange resin and anion exchange resin enrichment, purification, obtain Ramulus Mori active site;
Described storng-acid cation exchange resin is the strong acid type resin of crosslinked polystyrene system; Described anion exchange resin is the strong base resin of crosslinked polystyrene system.
2. preparation method according to claim 1, it is characterized in that, the operating procedure of S2 middle strong acidity cation exchange resin and anion exchange resin enrichment, purification is: stock solution adsorbed by storng-acid cation exchange resin, then the ammonia of 0.2 ~ 1N is used to carry out eluting, collect eluent evaporate to dryness, obtain sample 1; Again by sample 1 water dissolution, slow transit through strong-base anion-exchange resin, water elution, volatilize eluent and obtain sample 2, be i.e. Ramulus Mori active site.
3. preparation method according to claim 1, is characterized in that, described ammonia concn is 0.5N.
4. the Ramulus Mori active site that the preparation method described in any one of claim 1 ~ 3 prepares.
5. Ramulus Mori active site according to claim 4 in preparation treatment and prevent diabetes, application in the medicine of obesity, hyperlipidemia or viral infection.
6. application according to claim 5, is characterized in that, described Ramulus Mori active site is combined with excipient substance and is prepared into liquid preparation or solid preparation.
7. application according to claim 6, is characterized in that, described solid preparation is granule, tablet, capsule or drop pill; Described liquid preparation is oral liquid or injecting fluid preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310297505.4A CN103417634B (en) | 2013-07-16 | 2013-07-16 | A kind of Ramulus Mori active site and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310297505.4A CN103417634B (en) | 2013-07-16 | 2013-07-16 | A kind of Ramulus Mori active site and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103417634A CN103417634A (en) | 2013-12-04 |
| CN103417634B true CN103417634B (en) | 2015-08-26 |
Family
ID=49643375
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310297505.4A Expired - Fee Related CN103417634B (en) | 2013-07-16 | 2013-07-16 | A kind of Ramulus Mori active site and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103417634B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103755623B (en) * | 2014-01-03 | 2015-12-30 | 广州军区广州总医院 | The acid ethanol solution that a kind of response phase method is optimized extracts the method for 1-Deoxynojirimycin in Mulberry Leaves |
| KR101876805B1 (en) | 2016-10-18 | 2018-07-10 | 고려대학교 산학협력단 | The composition containing the ethanol extracts of Ramulus mori for preventing or treating obesity |
| CN111269171B (en) * | 2020-04-08 | 2023-05-05 | 劲牌持正堂药业有限公司 | Preparation method of high-purity 1-deoxynojirimycin |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1559539A (en) * | 2004-02-23 | 2005-01-05 | 南开大学 | Medicinal mixture possessing alpha glycocidase inhibiting activity and its use |
| CN1715271A (en) * | 2004-06-30 | 2006-01-04 | 车庆明 | Method for extracting and preparing N-methyl-1-deoxy noijirimycin |
| WO2008025249A1 (en) * | 2006-08-21 | 2008-03-06 | Institute Of Mataria Medica, Chinese Academy Of Medical Sciences | The use of the effective section of alkaloids from ramulus mori for preparation of a hypoglycemig agent |
-
2013
- 2013-07-16 CN CN201310297505.4A patent/CN103417634B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1559539A (en) * | 2004-02-23 | 2005-01-05 | 南开大学 | Medicinal mixture possessing alpha glycocidase inhibiting activity and its use |
| CN1715271A (en) * | 2004-06-30 | 2006-01-04 | 车庆明 | Method for extracting and preparing N-methyl-1-deoxy noijirimycin |
| WO2008025249A1 (en) * | 2006-08-21 | 2008-03-06 | Institute Of Mataria Medica, Chinese Academy Of Medical Sciences | The use of the effective section of alkaloids from ramulus mori for preparation of a hypoglycemig agent |
Non-Patent Citations (1)
| Title |
|---|
| 桑树资源中1-脱氧野尻霉素的含量测定及桑叶、桑枝和蚕沙中1-脱氧野尻霉素等多羟基生物碱的提取纯化技术研究;宋婕;《中国优秀硕士学位论文全文数据库 医药卫生科技辑》;20111115(第11期);第16页第2.6节、第17页表2-7、第19页第2.7.4节，第25页第最后1段，第19页第2.7.3节，第Ⅱ页第1段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103417634A (en) | 2013-12-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102617745B (en) | Preparation method and blood sugar lowering function of Ganoderma lucidum karst polysaccharide F31 | |
| CN104459003B (en) | The construction method of contracting spring standard preparation finger-print and characteristic spectrum and quality determining method | |
| CN103550265B (en) | Extraction method of active ingredients of tuckahoe peels and tuckahoe peel extracts | |
| CN103417634B (en) | A kind of Ramulus Mori active site and its preparation method and application | |
| CN103724445A (en) | Preparation method and blood sugar lowering function of Grifola frondosa polysaccharide F2 | |
| CN103623059B (en) | A kind of pharmaceutical composition and application thereof, containing its medicament | |
| CN102221590A (en) | Method for simultaneously determining multi-index ingredients of Simotang preparation and establishing fingerprint chromatogram thereof | |
| CN101862346B (en) | Hypoglycemic application of gingseng acidic polysaccharide | |
| CN105285643B (en) | A kind of preparation method of ginsenoside drink | |
| CN103565866A (en) | Preparation method of panax notoginseng saponins | |
| CN112526014B (en) | Jinyinliang oral liquid fingerprint spectrum and establishing method thereof | |
| CN102846704B (en) | A Leonurus japonicus injection, its preparation method, and method for detecting total alkaloids | |
| CN102526138B (en) | Composition of active components from fresh purslane for decreasing blood sugar and preparation method | |
| CN103816147B (en) | The medical usage of gamlogic acid, neogambogic acid and compositions thereof | |
| CN105535219A (en) | Xanthoceras sorbifolia bunge flavone extract as well as preparation method, quality detection method and application thereof | |
| CN108524811A (en) | Reduce uric acid, cholesterol, three high Chinese medicine compositions and its preparation method for the treatment of | |
| CN101618069B (en) | Capsule preparation for treating bruise as well as bleeding and easing pain and preparation method and use thereof | |
| CN103830374A (en) | Application of three-leaf glycolipid-removal medicine in hyperuricemia | |
| CN104161859B (en) | It is a kind of to be used to treat Chinese medicine composition of the spleen and stomach illness and preparation method thereof | |
| CN102430001B (en) | Compound rose-hip flavone preparation for preventing diabetes mellitus and preparation method thereof | |
| CN101744859B (en) | Pharmaceutical composition for promoting blood circulation, removing blood stasis and relieving pain and preparation method thereof | |
| CN101890077A (en) | Preparation method and quality detection method of rhizoma corydalis pain relieving soft capsules | |
| CN105663626A (en) | Rhizoma anemarrhenae effective part as well as preparation method and application thereof | |
| CN105377280B (en) | A kind of drug and preparation method thereof for enriching blood | |
| CN103690677B (en) | A kind of root bark of Chinese wolf-berry active site and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150826 Termination date: 20210716 |