CN103416694B - Method for producing instant kudzu vine root powder through direct enzymolysis of kudzu vine root raw materials by adoption of compound enzymes - Google Patents
Method for producing instant kudzu vine root powder through direct enzymolysis of kudzu vine root raw materials by adoption of compound enzymes Download PDFInfo
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Abstract
The invention provides a method for producing instant kudzu vine root powder through direct enzymolysis of kudzu vine root raw materials by the adoption of compound enzymes. The method comprises the following steps that firstly, fresh kudzu vine roots are washed, cut and dried at the temperature of 54 DEG C-56 DEG C until the moisture content is 5%-6%, the dried kudzu vine roots are smashed and screened with a 110-120-mesh sieve, and therefore kudzu vine root large-size powder is obtained, wherein the thickness of the cut kudzu vine roots is 3-6 mm; secondly, the kudzu vine root large-size powder is processed through cellulose and pullulanase simultaneously, wherein the dosage of the pullulanase is 17-19U/(g kudzu vine root large-size powder), the dosage of the cellulose is 1.18*104-1.22*104 U/(g kudzu vine root large-size powder), the substrate concentration is 14-16%, the enzymolysis time is 2.8-3.0 hours, the enzymolysis temperature is 54-56 DEG C; thirdly, the kudzu vine root large-size powder is placed in a boiling water bath for inactivation for 5 min after enzymolysis, and when the temperature of enzymatic hydrolysate is lowered to the enzymolysis temperature, centrifugation is conducted on the enzymatic hydrolysate at 3400-3500 rpm for 15-16 min; fourthly supernatant is collected, concentration, cooling and drying are conducted on the collected supernatant, and therefore the instant kudzu vine root powder is obtained. The method for producing the instant kudzu vine root powder through direct enzymolysis of the kudzu vine root raw materials by the adoption of the compound enzymes is simple in technology, moderate in reaction condition and high in product yield, the instant kudzu vine root powder is good in solubility, and the flavone reservation rate is high.
Description
Technical field
The present invention relates to a kind of preparation method of kudzu-vine root powder, particularly a kind of cellulase that utilizes produces the method for instant kudzu-vine root powder with the complex enzyme zymohydrolysis root of kudzu vine thin slice of enzyme polysaccharase composition of growing sturdily.
Background technology
The root of kudzu vine is the root of pulse family Papillionoideae Pueraria elegant jessamine or sweet Pueraria lobota, is mainly distributed in the temperate zone of height above sea level between 100-2000m and subtropical zone.There is Pueraria kind more than 20 in the whole world, is mainly distributed in the Asias such as China, India, Japan; China has 8 kinds of traditional Puerarias and 2 mutation, and the kind of current wild resource and introducing and planting has 15 kinds.Elegant jessamine all has distribution in most of provinces and regions of China except Tibet, Xinjiang, and Pachyrhizua angulatus is at present based on artificial cultivation.China's Pueraria lobota class is produced per year more than 1,500,000 tons, and resource has overwhelming superiority.Its small portion uses as medicinal material and produces pueraria starch, also has considerable resource not to be developed.
Kudzuvine root starch contains rich in protein and multiple essential amino acid and trace element, has and builds up health, promotion health, prophylactic effect.In recent years because the health care of the root of kudzu vine and economic worth are familiar with gradually, Pueraria lobota based article has received the concern of people.With kudzuvine root starch be the various food that make of raw material in international market extremely consumers especially in the Japanese market.All occur pueraria starch food processing enterprises both at home and abroad, but the pueraria starch produced is very different.The kudzuvine root starch that market is sold is wash-out starch out from the root of kudzu vine just, except the limitation of the starch component that only make use of in the root of kudzu vine, also have product directly not take after mixing it with water, must mix well with cold water the shortcoming disperseing could eat after the long period boils, eat and comparatively bother.Also there is the report of the research about instant kudzu vine powder at present, also have the kudzuvine root starch being labeled as " instant " even on the market, but its brew is unsatisfactory, become paste property bad, there is no the proterties of obvious transparent colloid.
The present invention is directed to existing instant kudzu vine powder to produce and just utilize the starch separated in the root of kudzu vine to carry out the poor technical bottleneck of modification, its product brew and the transparency for raw material, utilize cellulase to process root of kudzu vine raw material with enzyme polysaccharase mixed enzyme of growing sturdily simultaneously, namely exploitation one warm water can eat kudzu-vine root powder product once rushing, homogeneous, stable colloid can be formed after making kudzuvine root starch reconstitute simultaneously, improve it and reconstitute stability, the transparency and dispersed, possess good dissolubility.The present invention both developed new product, took full advantage of raw material again, improve rate processed, reduced cost, greatly improve value-added content of product, had great facilitation to mountain area economy, produced huge economic benefit and social benefit.
Summary of the invention
Technical problem to be solved by this invention is: only utilize ready-made pueraria starch to carry out modification for prior art, produce the brew of product and the poor technical bottleneck of dispersiveness, a kind of preparation method of instant kudzu-vine root powder of efficient, safety, low cost is provided.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of method utilizing complex enzyme direct enzymolysis root of kudzu vine raw material to produce instant kudzu-vine root powder, it comprises the following steps:
(1) root of kudzu vine pre-treatment: the thin slice being cut into 3mm-6mm thickness after being cleaned by the fresh root of kudzu vine, dries moisture for 5%-6% with constant temperature 54 DEG C-56 DEG C, pulverized 110 order-120 mesh sieves, and obtained root of kudzu vine meal.
(2) process root of kudzu vine meal with cellulase and enzyme polysaccharase of growing sturdily: root of kudzu vine meal is mixed with the root of kudzu vine meal solution that concentration of substrate is 14%-16% simultaneously, add cellulase and enzyme polysaccharase of growing sturdily simultaneously, wherein to grow sturdily enzyme polysaccharase consumption 17U-19U/(g root of kudzu vine meal), cellulase consumption 1.18 × 10U
4-1.22 × 10
4u/(g root of kudzu vine meal), enzymolysis 2.8h-3.0h is constantly stirred at temperature is 54 DEG C-56 DEG C, deactivation 5min in boiling water is placed on after enzymolysis, with the centrifugal 15min of 3400rpm-3500rpm-16min when enzymolysis liquid is cooled to 54 DEG C-56 DEG C, collect supernatant, concentrated frozen drying obtains instant kudzu-vine root powder.
Below to the test raw material used in the present invention, reagent, detection data, detection method and how to obtain the inventive method and be described:
1. the test raw material used and reagent
The root of kudzu vine (western Hunan a year and a half is raw), enzyme polysaccharase of growing sturdily (purchased from Shanghai Kai Yang Bioisystech Co., Ltd, Rate activity 5U/mg), cellulase (purchased from Shanghai Kai Yang Bioisystech Co., Ltd, Rate activity 400u/mg).
2. Detection of content and method
2.1 calculate reduced sugar equivalent (DE) value
Take 1.000g kudzuvine root starch in 100ml volumetric flask, add water 60ml, in 55 DEG C of water-baths, heat 2h, and concussion in every 5 minutes once.Constant volume after cooling, 20min is placed in mixing, 6000rpm, get supernatant after 20min is centrifugal, get 2ml in 25ml volumetric flask, add 1.5mlDNS reagent and take out after water-bath 5min in boiling water, ice bath is cooled to room temperature, be settled to 25mL with distilled water, put upside down mixing, at 540nm wavelength, place surveys its absorbance.With blank zeroing, make calibration curve with glucose standard specimen, obtain the content of reduced sugar in sample.
DE=glucidtemns content of reducing sugar × 100/ dry matter weight
The mensuration of 2.2 root of kudzu vine solubility
Claim the instant kudzu-vine root powder of 30.0 g, add 100 ml, 60 DEG C of warm waters, in the centrifugal 15min of 3000rpm after abundant stirring 5 min, incline and centrifuge tube supernatant, with a small amount of water, sediment is washed in the weighing disk of constant after carefully wiping pigging wall with tampon, first evaporate to dryness in water-bath, then in 100 DEG C of drying 1 h, put cool to room temperature in drier, weigh, again after 100 DEG C of drying 30 min, take out cooling and weigh, to straight constant weight.
Result calculates
X（%）=100-[(M
1– M
2)*100/M
3]
In formula: X: the solubility (%) of sample; M
1: weighing disk adds insoluble matter quality (g); M
2: weighing disk quality (g); M
3: sample mass (g).
The mensuration of 2.3 Pueraria Flavonids
Make standard items with rutin and adopt alchlor colorimetric method for determining.
3. test procedure
3.1 root of kudzu vine pre-treatments
Be cut into the thin slice of 3mm-6mm thickness after being cleaned by the fresh root of kudzu vine, dry moisture for 5%-6% with constant temperature 54 DEG C-56 DEG C, pulverized 110 order-120 mesh sieves, and obtained root of kudzu vine meal.
3.2 directly produce instant kudzu-vine root powder with root of kudzu vine meal
Take true a certain amount of Pueraria lobota meal, add appropriate warm water, being made into root of kudzu vine meal solution concentration is 14%-16%, constantly stirs in process of the test.Under 54 DEG C of-56 DEG C of conditions, be incubated 2.8h-3.0h, be incubated 5min afterwards in boiling water bath, with the centrifugal 15min of 3500 rpm-16min when being cooled to 54 DEG C-56 DEG C, collect supernatant, concentrated frozen drying obtains instant kudzu-vine root powder.The DE value 15.03-15.32 of kudzuvine root starch, yield is 2.75-3.02%, and solubility is 10.90%-11.13%, and flavones retention rate is 58.11%-58.83%.
3.3 grow sturdily enzyme polysaccharase hydrolysis the root of kudzu vine produce instant kudzu-vine root powder
By the single factor experiment of the concentration of substrate to enzyme polysaccharase hydrolysis root of kudzu vine meal of growing sturdily, enzyme dosage, enzymolysis time, hydrolysis temperature, determine the optimum range of each factor, design orthogonal test, for making enzyme-to-substrate fully contact, constantly stirs in process of the test.It is concentration of substrate 14%-16% that orthogonal test obtains hydrolysis result optimum combination, enzyme dosage 17 U-19 U/ (g kudzuvine root starch), enzymolysis time 2.6h-2.8h, hydrolysis temperature 55 DEG C-58 DEG C.Be placed on deactivation 5min in boiling water bath after enzymolysis, with the centrifugal 15 min-16min of 3400rpm-3500rpm when enzymolysis liquid is cooled to hydrolysis temperature, collect supernatant, concentrated postlyophilization obtains instant kudzu-vine root powder.Obtain the DE value of kudzuvine root starch product for 44.16-45.50, yield is 33.09%-34.11%, and solubility is 17.91%-18.17%, and flavones retention rate is 52.13%-53.02%.
The 3.4 cellulose hydrolyzation roots of kudzu vine produce instant kudzu-vine root powder
By the single factor experiment of the concentration of substrate to cellulose hydrolyzation root of kudzu vine meal, enzyme dosage, enzymolysis time, hydrolysis temperature, determine the optimum range of each factor, design orthogonal test, for making enzyme-to-substrate fully contact, constantly stirs in process of the test.It is concentration of substrate 14%-16% that orthogonal test obtains hydrolysis result optimum combination, enzyme dosage 1.18 × 10
4u-1.22 × 10
4u/(g kudzuvine root starch), enzymolysis time 2.8h-3.0h, hydrolysis temperature 54 DEG C-56 DEG C.Deactivation 5min in boiling water bath is placed on after enzymolysis, with the centrifugal 15min of 3400rpm-3500rpm-16min when enzymolysis liquid is cooled to hydrolysis temperature, obtain the DE value of kudzuvine root starch product for 33.18-35.34, yield is 31.43%-32.55%, solubility is 15.21%-15.07%, and flavones retention rate is 58.02%-59.18%.
The 3.5 complex enzyme for hydrolyzing roots of kudzu vine produce instant kudzu-vine root powder
The result of above-mentioned test 3.2-3.4 absolutely proves that adding enzyme hydrolysis root of kudzu vine raw material produces instant kudzu-vine root powder and have a significant effect, if but in process, carry out enzymolysis in two steps can extend process time, increase production cost.Because of two kinds of zymolyte differences, mutually do not compete and disturb, thus can carry out enzymolysis simultaneously.The mode that employing complex enzyme hydrolysis is added in two kinds of enzyme mixing is hydrolyzed by the optimum enzymatic hydrolysis condition with reference to two kinds of enzymes, fully contact for making enzyme-to-substrate, constantly stir in process of the test, obtaining optimum complex enzyme hydrolysis technique is: enzyme polysaccharase consumption 17 U-19U/(g kudzuvine root starch of growing sturdily), cellulase consumption 1.18 × 10
4u-1.22 × 10
4u/(g kudzuvine root starch), concentration of substrate is 14%-16%, enzymolysis time 2.8 h-3.0h, hydrolysis temperature 54 DEG C-56 DEG C, deactivation 5min in boiling water bath is placed on after enzymolysis, with the centrifugal 15min-16min of 3400rpm-3500rpm when enzymolysis liquid is cooled to hydrolysis temperature, collect supernatant, concentrated frozen drying obtains instant kudzu-vine root powder.After enzymolysis, the DE value of kudzuvine root starch reaches 50.33-51.65, and yield is 42.35%-44.66%, and solubility is 20.90%-21.13%, and flavones retention rate is 50.34%-50.78%.
4. novelty
Relative to the production technology of current kudzu-vine root powder, the present invention has following novelty:
4.1 technology of the present invention make full use of raw material, the technology of developing new product efficiently, at low cost
The instant-dissolve kudzuvine root powder of current report is produced and is just adopted wash-out starch out from the root of kudzu vine to be that raw material carries out modification preparation, and be not real " instant ", the present invention then adopts the complex enzyme direct enzymolysis root of kudzu vine raw material be made up of cellulase and enzyme polysaccharase of growing sturdily to be prepared instant kudzu-vine root powder, make full use of the starch in root of kudzu vine raw material and the multiple component content such as cellulose, hemicellulose, decrease pueraria starch wash-out and prepare this procedure and residue discharge, reduce production cost, the production efficiency of raising.
4.2 effects making full use of complex enzyme, reaction condition is gentle, environmental friendliness
What the present invention adopted is that cellulase and enzyme polysaccharase compound of growing sturdily carry out enzymolysis, and enzymolysis time is short, and the temperature that production process adopts is not high, and reaction condition is gentle, has saved the energy; Overall process does not use other any chemical reagent, avoids environmental pollution, is environmental friendliness project.
4.3 product yield are high, and solubility property is good, quality better
There is one layer of cells wall in root of kudzu vine extracellular, and form primarily of cellulose, cellulase can promote cellulose, hemicellulose resolves into small-molecule substance, promotes the dissolving of plant cell wall, more cell inclusion is dissolved out; Containing amylopectin and amylose, mainly amylopectin in pueraria starch, and be all insoluble in cold water and warm water, enzyme polysaccharase of growing sturdily selectivity can cut α-1,6 glycosidic bond in amylopectin branch point, cuts whole branched structure, forms amylose.By adding cellulase and enzyme polysaccharase of growing sturdily can hydrocellulose and starch, its substrate specificity is different, and fully hydrolysis two large class polysaccharide molecule materials, form small-molecule substance, raising dissolubility.Whole process relative low temperature, and by improving enzymolysis liquid centrifugal rotational speed, reduce insoluble composition in supernatant, product dissolubility improves a lot.Can form homogeneous, stable colloid after kudzuvine root starch reconstitutes, it reconstitutes stability, the transparency and dispersed raising, and possesses good instant capacity.Flavones is the effective constituents in the root of kudzu vine, and in traditional kudzu-vine root powder preparation section, the overwhelming majority is all stayed in pueraria root residue discarded, and the present invention remains more kudzu root flavone while improving the dissolution characteristics of pueraria starch.
Detailed description of the invention
Embodiment one
By the fresh root of kudzu vine clean after peeling, be cut into the thin slice of 4mm thickness, with 55 DEG C of constant temperature dryings to biodiversity percentage composition for 5%, pulverized 120 mesh sieves, be prepared into root of kudzu vine meal.
Take root of kudzu vine meal 300.0 g, 1700 ml that add water are mixed with the root of kudzu vine meal solution that concentration of substrate is 15%, and the addition of enzyme polysaccharase of growing sturdily is 18U/g substrate and cellulase consumption is 1.2 × 10
4u/g substrate, constantly stirs enzymolysis time 3.0 h, is then placed on deactivation 5min in boiling water bath under temperature 55 DEG C of conditions, and with centrifugal 15 min of 3500 rpm after being cooled to 55 DEG C, collect supernatant, concentrated frozen drying obtains instant kudzuvine root starch.The DE value measuring instant kudzu-vine root powder reaches 50.44, and flavones reserved is 50.31%, and yield is 44.24 %, and solubility is 21.06%.Instant kudzu-vine root powder is dissolved into stable colloidal.
Embodiment two
By the fresh root of kudzu vine clean after peeling, be cut into the thin slice of 5 mm thickness, with 55 DEG C of constant temperature dryings to biodiversity percentage composition for 6%, pulverized 110 mesh sieves, be prepared into root of kudzu vine meal.
Take root of kudzu vine meal 450.0 g, 2550 ml that add water are mixed with the root of kudzu vine meal solution that concentration of substrate is 15%, and the addition of enzyme polysaccharase of growing sturdily is 19 U/g substrates and cellulase consumption is 1.18 × 10
4u/g substrate, constantly stirs enzymolysis time 2.9 h, is then placed on deactivation 5 min in boiling water bath under temperature 54 DEG C of conditions, with the centrifugal 15min of 3400 rpm after being cooled to 54 DEG C, collects supernatant, and concentrated frozen is dry obtains instant kudzuvine root starch.The DE value measuring instant kudzu-vine root powder reaches 51.03, and flavones reserved is 50.37%, and yield is 43.79 %, and solubility is 20.86%.Instant kudzu-vine root powder is dissolved into stable colloidal.
Claims (1)
1. utilize complex enzyme direct enzymolysis root of kudzu vine raw material to produce a method for instant kudzu-vine root powder, it is characterized in that comprising the following steps:
(1) root of kudzu vine pre-treatment: the thin slice being cut into 3mm-6mm thickness after being cleaned by the fresh root of kudzu vine, dries moisture for 5%-6% with constant temperature 54 DEG C-56 DEG C, pulverized 110 order-120 mesh sieves, and obtained root of kudzu vine meal;
(2) process root of kudzu vine meal with cellulase and enzyme polysaccharase of growing sturdily: root of kudzu vine meal is mixed with the root of kudzu vine meal solution that concentration of substrate is 14%-16% simultaneously, add cellulase and enzyme polysaccharase of growing sturdily simultaneously, wherein to grow sturdily enzyme polysaccharase consumption 17U-19U/(g root of kudzu vine meal), cellulase consumption 1.18 × 10
4u-1.22 × 10
4u/(g root of kudzu vine meal), enzymolysis 2.8h-3.0h is constantly stirred at temperature is 54 DEG C-56 DEG C, deactivation 5min in boiling water bath is placed on after enzymolysis, with 3400rpm-3500 rpm centrifugal 15min-16 min when enzymolysis liquid is cooled to hydrolysis temperature 54 DEG C-56 DEG C, collect supernatant, concentrated frozen drying obtains instant kudzu-vine root powder.
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| CN104026513A (en) * | 2014-06-05 | 2014-09-10 | 湖南中医药大学 | Method for preparing instant kudzuvine root whole powder by complex enzyme process |
| CN106418496A (en) * | 2016-08-09 | 2017-02-22 | 三峡大学 | Process for producing instant kudzuvine root powder by use of enzymolysis method |
| JP7222911B2 (en) | 2017-04-14 | 2023-02-15 | カプスゲル・ベルギウム・ナムローゼ・フェンノートシャップ | How to make pullulan |
| AU2018251256B2 (en) | 2017-04-14 | 2023-10-05 | Capsugel Belgium Nv | Pullulan capsules |
| CN109090413B (en) * | 2018-07-20 | 2022-04-15 | 江南大学 | Preparation method of mung bean and kudzu root instant beverage powder |
| CN109078042B (en) * | 2018-08-14 | 2022-05-31 | 齐鲁工业大学 | Method for extracting active ingredients from radix puerariae through compound biological enzymolysis |
| CN110720629A (en) * | 2019-11-15 | 2020-01-24 | 湖南博嘉魔力农业科技有限公司 | Processing method of kudzu vine root and konjac glucomannan composite chewable matter |
| CN111067055A (en) * | 2019-12-26 | 2020-04-28 | 武汉轻工大学 | Instant kudzu root whole powder and preparation method thereof |
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