CN1034135A - The preparation method of peganum harmala seed extract - Google Patents
The preparation method of peganum harmala seed extract Download PDFInfo
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- CN1034135A CN1034135A CN 87104598 CN87104598A CN1034135A CN 1034135 A CN1034135 A CN 1034135A CN 87104598 CN87104598 CN 87104598 CN 87104598 A CN87104598 A CN 87104598A CN 1034135 A CN1034135 A CN 1034135A
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- peganum harmala
- seed
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- seed extract
- ethanol
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Abstract
The invention provides a kind of method for preparing peganum harmala seed extract.The present invention adopts the aquiferous ethanol of 40%-95% (W/W) that seed of peganum harmala is extracted.Production technology of the present invention is simple, nontoxic, safety, and cost is low, and the active ingredient extraction ratio is higher, and water-solubility impurity is low, saves man-hour, and after extraction was finished, ethanol can reclaim fully, and can reuse.Peganum harmala seed extract is to make antitumor, the raw material of medicines such as antiulcer.
Description
The invention provides a kind of method for preparing peganum harmala seed extract.
Herba pegani harmalae (Peganum harmala L.Zygophyllaceae) belongs to the zygophyllaceae herbaceos perennial.Main active ingredient is in the seed of peganum harmala: banisterine (Harmaline), yageine (Harmine), Harmalol (Harmalol) and peganine. (Vasicine) etc. are referred to as total alkaloid of harmaline (Total alkaloid harmala).
The present invention once found banisterine in 1841 by lid Bel abroad in the past, found yageine by Fu Liteqi in 1847, but extraction separation method was not quite clear.Nineteen twenty-seven have again Bai Qin and Robinson adopt between-methoxybenzene diazonium chloride and phosphorus phthalimido-acetyl group-penta vinegar ester through the complicated approach synthetic banisterine and yageine (Su Aoliehefu shows; the alkaloid chemistry; Beijing Science Press; 1960; 504～506); but, have not yet to see report both at home and abroad to the preparation method of peganum harmala seed extract.
The invention provides and a kind ofly need not hang down utmost point organic solvent, produce peganum harmala seed extract, with the output that improves active substance and reduce production costs, make full use of abundant seed of peganum harmala resource, be suitable for the preparation method of suitability for industrialized production with ethanol.
The present invention adopts 40%-95%(W/W) aquiferous ethanol seed of peganum harmala is extracted.
The used ethanol of the present invention is the mixture of alcohol and water.Because concentration of ethanol is excessive, organic molecule impurity such as polysaccharide, protein is corresponding is leached, and causes the difficulty of granulating, and concentration of alcohol is low excessively, and the water-solubility impurity effective component contents that extract low more, and cost consumption is big.Alcoholic acid weight can be to account for 40%-95%, is preferably 60%-90%, is preferably 75%-85%.
Active ingredient in the seed of peganum harmala can extract with several different methods, for example infusion process, percolation, ion exchange, but in order to improve the output of active substance, extraction process is preferably the hot reflux extraction.
Hot reflux is extracted 3-5 time, counts 3-5 hour, because alcoholic acid boiling point is 78.3 ℃, the hot reflux temperature adopts the decompression hot reflux also can at 75 ℃-85 ℃ certainly.
In preparation peganum harmala seed extract process, whenever seed of peganum harmala is not placed in more than 100 ℃.
Seed of peganum harmala is the ripe exsiccant seed of zygophyllaceae herbaceos perennial Herba pegani harmalae.
Peganum harmala seed extract contains two kinds of alkaloids, i.e. banisterine and yageine at least.
Production technology of the present invention is simple, nontoxic, safety, and cost is low, and the active ingredient extraction ratio is higher, and water-solubility impurity is low, saves man-hour, and after extraction was finished, ethanol can reclaim fully, and can reuse.
Discrimination method:
Get extractum 1 gram,, get filtrate and do following test with 5% dilute hydrochloric acid or spirit of vinegar 50ml dissolution filter:
1. get 1 of filtrate or 2ml and place on the spot plate or in vitro, add reagent such as potassium mercuric iodide, bismuth potassium iodide, silico-tungstic acid and generate reddish brown precipitation.
2. filtrate presents intensive blue green fluorescence under sunlight or uviol lamp.
3. thin layer chromatography, condition: silica gel G plate, 105 ℃ of activation 1 hour, developing solvent: CHCL
3: EtOH(6.3: 3.7) under uviol lamp, should present 2 above fluorescence speckles.
Embodiment 1
The ripe dry seed of 10kg Herba pegani harmalae (picking up from the Urumchi Western Hills) is broken slightly, it is inserted in the extractor, add 40% ethanol 20kg-30kg, reflux, extract 5 times, each 1 hour, temperature got extracting solution at 75 ℃-85 ℃, reclaim ethanol, get thick body of paste, promptly extractum 1.37kg presses the mensuration mensuration total alkaloid content 15.0% of known heavy.When hot reflux extraction 3 times, and other condition is identical, gets extractum 1.23kg, total alkaloid content 13.6%
Embodiment 2
The ripe dry seed of 10kg Herba pegani harmalae (picking up from the Urumchi Western Hills) is broken slightly, it is inserted in the extractor, add 60% ethanol 20kg-30kg, reflux, extract 5 times, each 1 hour, temperature got extracting solution at 75 ℃-85 ℃, reclaim ethanol, get thick body of paste, promptly extractum 1.26kg presses the mensuration mensuration total alkaloid content 17.2% of known heavy.When hot reflux extraction 3 times, and other condition is identical, gets extractum 1.14kg, total alkaloid content 14.8%
Embodiment 3
The ripe dry seed of 10kg Herba pegani harmalae (picking up from the Urumchi Western Hills) is broken slightly, it is inserted in the extractor, add 80% ethanol 20kg-30kg, reflux, extract 5 times, each 1 hour, temperature got extracting solution at 75 ℃-85 ℃, reclaim ethanol, get thick body of paste, promptly extractum 1.21kg presses the mensuration mensuration total alkaloid content 20.0% of known heavy.When hot reflux extraction 3 times, and other condition is identical, gets extractum 1.08kg, total alkaloid content 16.4%
Embodiment 4
The ripe dry seed of 10kg Herba pegani harmalae (picking up from the Urumchi Western Hills) is broken slightly, it is inserted in the extractor, add 95% ethanol 20kg-30kg, reflux, extract 5 times, each 1 hour, temperature got extracting solution at 75 ℃-85 ℃, reclaim ethanol, get thick body of paste, promptly extractum 1.15kg presses the mensuration mensuration total alkaloid content 25.5% of known heavy.When hot reflux extraction 3 times, and other condition is identical, gets extractum 0.85kg, total alkaloid content 23.1%
Claims (6)
1, a kind of preparation method of peganum harmala seed extract is characterized in that the method adopts the aquiferous ethanol of 40%--95% (W/W) that seed of peganum harmala is extracted.
2, preparation method according to claim 1 is characterized in that extraction process is preferably the hot reflux extraction.
3, preparation method according to claim 2 is characterized in that hot reflux extraction 3-5 time, counts 3-5 hour, and the hot reflux temperature is at 75 ℃-85 ℃.
4, preparation method according to claim 1 is characterized in that whenever seed of peganum harmala is not placed in more than 100 ℃.
5, preparation method according to claim 1 is characterized in that seed of peganum harmala is the ripe exsiccant seed of zygophyllaceae herbaceos perennial Herba pegani harmalae.
6, preparation method according to claim 1 is characterized in that peganum harmala seed extract contains two kinds of alkaloids, i.e. banisterine and yageine at least.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87104598 CN1034135A (en) | 1987-10-27 | 1987-10-27 | The preparation method of peganum harmala seed extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87104598 CN1034135A (en) | 1987-10-27 | 1987-10-27 | The preparation method of peganum harmala seed extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1034135A true CN1034135A (en) | 1989-07-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 87104598 Pending CN1034135A (en) | 1987-10-27 | 1987-10-27 | The preparation method of peganum harmala seed extract |
Country Status (1)
| Country | Link |
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| CN (1) | CN1034135A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999024048A1 (en) * | 1997-11-06 | 1999-05-20 | Pharmaceutical Research Center | Cancer treatment drug |
| CN104628731A (en) * | 2015-01-27 | 2015-05-20 | 中国农业科学院兰州畜牧与兽药研究所 | Method for extracting peganum harmala alkaloid under microwave assistance |
-
1987
- 1987-10-27 CN CN 87104598 patent/CN1034135A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999024048A1 (en) * | 1997-11-06 | 1999-05-20 | Pharmaceutical Research Center | Cancer treatment drug |
| CN104628731A (en) * | 2015-01-27 | 2015-05-20 | 中国农业科学院兰州畜牧与兽药研究所 | Method for extracting peganum harmala alkaloid under microwave assistance |
| CN104628731B (en) * | 2015-01-27 | 2017-02-22 | 中国农业科学院兰州畜牧与兽药研究所 | Method for extracting peganum harmala alkaloid under microwave assistance |
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| RJ01 | Rejection of invention patent application after publication |