CN103408398A - Method for extracting Falcarindiol from radix glehniae - Google Patents

Abstract

The invention discloses a method for extracting Falcarindiol from radix glehniae. The method is as below: 1) crushing radix glehniae into 20-80 mesh, extracting the radix glehniae in a CO2 supercritical extraction tank, introducing liquid CO2 with flow of 1-5 ml/min/ g raw material at a pressure of 15-25 MPa, extracting for 1-3 h and parsing to obtain an extract; 2) dissolving the extract with methanol solution, passing the solution through an alumina column, concentrating a lower column liquid, separating the concentrated solution with high speed counter current chromatogram by using a hexane-ethyl acetate-methanol-water solvent system, collecting flow components according to the atlas, merging the flow components, and drying under reduced pressure to obtain Falcarindiol. The method for producing Falcarindiol has advantages of high extraction efficiency, simple technology operation and low pollution, and is suitable for production of high purity Falcarindiol.

Description

A kind of method from extraction method card woods glycol Root of coastal Glehnia

Technical field

The invention belongs to biological technical field, be specifically related to a kind of method from extraction method card woods glycol Root of coastal Glehnia.

Background technology

The Fa Kalin glycol is alkynol class material, and CAS 55297-87-5, molecular weight are C17H24O2, and molecular weight is 260.37.Molecular structural formula:

Root of coastal Glehnia is samphire Phellopterus littoralis (Glehnia lit-toralis F.Schmidt ex Miq.), is used as medicine with root.The Root of coastal Glehnia flavor is sweet, is clinical drugs for nourishing yin commonly used, nourishing YIN and clearing away lung-heat, expelling phlegm for arresting cough.Cure mainly dryness of the lung dry cough, consumption of body fluid caused by febrile disease, the disease such as thirsty.Modern study is found, in Root of coastal Glehnia, contains tonka bean camphor, alkynol, glycoside, polysaccharide, organic acid etc., and wherein the Fa Kalin glycol is the index composition in Root of coastal Glehnia, has antibiotic, anti-inflammatory, antitumor, antifatigue and the multiple effect such as calm.

By literature search, Fa Kalin glycol extracting method adopts organic reagent to extract more and silicagel column separates, and this class methods extraction efficiency is low, the purge process complexity, and product yield is low.

Summary of the invention

The technical problem to be solved in the present invention is to provide a kind of method from extraction method card woods glycol Root of coastal Glehnia easy and simple to handle.

The present invention is achieved by the following technical solutions:

A kind of method from extraction method card woods glycol Root of coastal Glehnia is characterized in that comprising the following steps:

1) get Root of coastal Glehnia and pulverize the 20-80 order, be placed in CO2 supercritical extraction tank and extract, pass into liquid CO 2, flow is the 1-5ml/min/g raw material, pressure 15-25Mpa, and extraction time 1-3h, resolve to obtain extract;

2) above-mentioned extract dissolve with methanol solution, peroxidation aluminium post, lower column liquid is concentrated, and concentrated solution be take normal hexane-ethyl acetate-methanol-water and is solvent systems, separates with high speed adverse current chromatogram, collects flow point according to collection of illustrative plates, merges the flow point drying under reduced pressure and get final product.

Described step 2) normal hexane-ethyl acetate in-methanol-water blending ratio is: 3-7:2-5:2-7:3-7, and get and be mutually stationary phase, lower is moving phase mutually.

Adopt technique scheme working system card woods glycol, extraction efficiency is high, and technique is simple to operation, and environmentally friendly, is easy to realize suitability for industrialized production.

Embodiment

The invention will be further described below in conjunction with specific embodiment.

Embodiment 1:

Get Root of coastal Glehnia 2kg and dry, pulverized 40 mesh sieves, be placed in CO2 supercritical extraction tank and extract, pass into liquid CO 2, flow is the 3ml/min/g raw material, pressure 20Mpa, and extraction time 2h, resolve to obtain extract 80% dissolve with methanol solution, peroxidation aluminium post, lower column liquid is concentrated, obtains concentrated solution.Get normal hexane-ethyl acetate-methanol-water and mix by 3:5:2:3, get and be mutually to inject the high speed adverse current chromatogram pipe by stationary phase, open and turn main frame, rotating speed 800rpm, take off and do mutually moving phase, flow velocity 2ml/min, after setting up running balance, sample introduction, collect flow point according to collection of illustrative plates, merges the flow point drying under reduced pressure, obtain oily matter 3.2g, content 98.5%.

Embodiment 2:

Get Root of coastal Glehnia 2kg and dry, pulverized 60 mesh sieves, be placed in CO2 supercritical extraction tank and extract, pass into liquid CO 2, flow is the 1ml/min/g raw material, pressure 25Mpa, and extraction time 3h, resolve to obtain extract 90% dissolve with methanol solution, peroxidation aluminium post, lower column liquid is concentrated, obtains concentrated solution.Get normal hexane-ethyl acetate-methanol-water and mix by 4:3:5:4, get and be mutually to inject the high speed adverse current chromatogram pipe by stationary phase, open and turn main frame, rotating speed 900rpm, take off and do mutually moving phase, flow velocity 3ml/min, after setting up running balance, sample introduction, collect flow point according to collection of illustrative plates, merges the flow point drying under reduced pressure, obtain oily matter 3.7g, content 97.3%.

Embodiment 3:

Get Root of coastal Glehnia 2kg and dry, pulverized 80 mesh sieves, be placed in CO2 supercritical extraction tank and extract, pass into liquid CO 2, flow is the 2ml/min/g raw material, pressure 15Mpa, and extraction time 2h, resolve to obtain extract 80% dissolve with methanol solution, peroxidation aluminium post, lower column liquid is concentrated, obtains concentrated solution.Get normal hexane-ethyl acetate-methanol-water and mix by 5:3:5:3, get and be mutually to inject the high speed adverse current chromatogram pipe by stationary phase, open and turn main frame, rotating speed 800rpm, take off and do mutually moving phase, flow velocity 1ml/min, after setting up running balance, sample introduction, collect flow point according to collection of illustrative plates, merges the flow point drying under reduced pressure, obtain oily matter 2.8g, content 96.7%.

Claims (2)

1. method from extraction method card woods glycol Root of coastal Glehnia is characterized in that comprising the following steps:

1) get Root of coastal Glehnia and pulverize the 20-80 order, be placed in CO2 supercritical extraction tank and extract, pass into liquid CO 2, flow is the 1-5ml/min/g raw material, pressure 15-25Mpa, and extraction time 1-3h, resolve to obtain extract;

2) above-mentioned extract dissolve with methanol solution, peroxidation aluminium post, lower column liquid is concentrated, and concentrated solution be take normal hexane-ethyl acetate-methanol-water and is solvent systems, separates with high speed adverse current chromatogram, collects flow point according to collection of illustrative plates, merges the flow point drying under reduced pressure and get final product.

2. the method from extraction method card woods glycol Root of coastal Glehnia according to claim 1, is characterized in that described step 2) in normal hexane-ethyl acetate-methanol-water blending ratio be: 3-7:2-5:2-7:3-7, get and be mutually stationary phase, lower is moving phase mutually.