CN103394123A - Preparation method of CaO-SiO2-Ag2O/PAA compound biological membrane material - Google Patents
Preparation method of CaO-SiO2-Ag2O/PAA compound biological membrane material Download PDFInfo
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- CN103394123A CN103394123A CN2013103606216A CN201310360621A CN103394123A CN 103394123 A CN103394123 A CN 103394123A CN 2013103606216 A CN2013103606216 A CN 2013103606216A CN 201310360621 A CN201310360621 A CN 201310360621A CN 103394123 A CN103394123 A CN 103394123A
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Abstract
The invention relates to a preparation method of a CaO-SiO2-Ag2O/PAA compound biological membrane material. The preparation method comprises following steps: adding nitric acid in deionized water, evenly stirring, adding water solution with nitric acid in the silicon source with stirring, obtaining the SiO2 sol; dropwise adding the SiO2 sol into a mixed nitrate solution of silver and calcium, stirring for reactions for 40 to 65 minutes at the temperature of 20 to 40 DEG C, obtaining the sol; putting a PAA film in the sol, keeping for 2 to 4 hours at the condition of 0.085 MPa to 0.1 MPa, then taking out the PAA film, washing, drying, burning so as to obtain the product. The preparation method has the advantages of simple and practical technology, low cost and convenient promotion. The prepared CaO-SiO2-Ag2O/PAA has high antibiosis efficiency, and the bacteriostasis rates for escherichia coli and staphylococcus aureus are both over 90%.
Description
Technical field
The invention belongs to the preparation field of compound bio membrane material, particularly a kind of CaO-SiO
2-Ag
2The preparation method of O/PAA compound bio membrane material.
Background technology
Porous anodic aluminium oxide (PAA) film is the aluminum oxide film with highly regular pore structure that the method by electrochemical oxidation forms at surface of pure aluminum.The PAA thin film has chemical stability preferably, excellent abrasion-proof rust-preventing performance, and after implanting, have good biological safety, have no side effect, thereby be introduced into technical field of biological material gradually.Karlsson etc. (Biomaterials24 (2003) 3039-3046) study the interaction between cell and nano porous anodised aluminum with the HOB cell as cell culture model, result shows, PAA has good biocompatibility, is a kind of potential biomedical material.In addition, PAA has the nano pore structure of the large tracts of land hexagonal array that is parallel to each other, high specific surface area, and pore-size distribution homogeneous and controlled, thereby can be used as the excellent carrier of bioactive substance, thus give nanostructured itself special biological function.
Numerous research in recent years shows, calcium-silica-based (CaO-SiO
2) biomaterial has good biological activity and degradability, the rapid induction osteoid apatite deposits and promotes propagation and the differentiation of osseous tissue relevant cell in vitro and in vivo, damaged quick reparation is very important for osseous tissue for this, further research shows that calcium-silica-base material compares calcium-phosphorio material and have better one-tenth bone bioactivity, and as filling and repair materials, is applied on osseous tissue and joint.In addition, silver ion is to apply the earliest one of also the most ripe inorganic antiseptic, has the advantages such as safe, long-acting lasting and stability.Because silver ion has broad-spectrum bactericidal action and effective antibiotic concentration is lower, the people's such as Bellantone (Antimicrob Agents Chemother, 2002,6:1940～1945) research shows, as long as the concentration of silver ion is controlled at 0.1 * 10
-6G/L～1.6 * 10
-6Between g/L, just can give full play to its antibiotic property and obtain simultaneously biocompatibility preferably.Therefore, implant surface is carried the design of silver coating and is applied and caused people's extensive concern.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of CaO-SiO
2-Ag
2The preparation method of O/PAA compound bio membrane material, the method is quick, easy, efficient, is suitable for being applied to the coating modified design of biomaterial surface; The composite film material that this invention makes has special nano-pore structure, good biological activity and antibiotic property.
A kind of CaO-SiO of the present invention
2-Ag
2The preparation method of O/PAA compound bio membrane material comprises:
(1) nitric acid dropping is entered in deionized water, stir, obtain the aqueous solution of nitric acid acidify, then, while stirring to dripping the aqueous solution of nitric acid acidify in the silicon source, finally obtain SiO
2Colloidal sol; Wherein the volume ratio of silicon source, deionized water and nitric acid is 1:(8～12): (0.15～0.30);
(2) silver nitrate solution is added in calcium nitrate solution, mix homogeneously, obtain the nitrate mixed solution of silver, calcium; Then by SiO
2Colloidal sol is added drop-wise in the nitrate mixed solution of silver, calcium, and under 20-40 ℃ of condition, stirring reaction 40-65min, obtain CaO-SiO
2-Ag
2O colloidal sol; CaO-SiO wherein
2-Ag
2In O, the mass percent of three components is (45～55): (44.5～52): (0.5～3);
(3) the PAA thin film is placed in to above-mentioned CaO-SiO
2-Ag
2In O colloidal sol, then, under 0.085MPa～0.1MPa condition, keep 2-4h, then take out, washing, drying, then roasting under protective gas atmosphere, obtain CaO-SiO
2-Ag
2O/PAA compound bio membrane material.
In described step (1), the silicon source is ethyl orthosilicate, methyl silicate or butyl silicate.
In described step (2), the concentration of silver nitrate solution is 0.05mol/L-0.2mol/L, and the concentration of calcium nitrate solution is 2mol/L-3mol/L.
In described step (3), the aperture of PAA thin film is 100nm-250nm.
In described step (3), washing is for using ethanol and deionized water wash 3-5 time.
In described step (3), baking temperature is 65 ℃-130 ℃, and be 5-12h drying time.
In described step (3), protective gas is nitrogen or argon.
Roasting is roasting in Muffle furnace in described step (3), and the heating rate of Muffle furnace is 1 ℃/min-4 ℃/min, and sintering temperature is 400 ℃-550 ℃, and roasting time is 3-6h.
Synthesis of ternary is assembled CaO-SiO altogether
2-Ag
2The method of O is sol-gel process.
The present invention is used in the structure of PAA nano surface porous, with calcium-silica-based (CaO-SiO
2) bioactive particles is carrier, design calcium-silicon-Yin (CaO-SiO
2-Ag
2O) bioactive particles of ternary system, the bioactive particles of this ternary system is loaded in the duct of PAA, give the better biological activity of material surface, active ion sustained release performance and good anti-microbial property, this has important theory and medical value on clinical medicine.
In the nano pore of PAA, successfully loaded CaO-SiO
2-Ag
2The O bioactive particles, construct and namely have the bioactivity surface of inducing the osteoid apatite deposition capability to have again effective antibiotic property, and this active surface is expected to as a kind of face coat of novel Artificial Intervention material and is applied to technical field of biological material.
This compound bio membrane material soaks in simulated body fluid (SBF) after, the osteoid apatite deposition can be induced in surface; Antibacterial experiment shows that this material is respectively 90% and 92% to the bacteriostasis rate of escherichia coli and staphylococcus aureus.
2. the sign of material and Bioactivity evaluation
2.1PAA, CaO-SiO
2/ PAA and CaO-SiO
2-Ag
2The Microstructure characterization of O/PAA
Adopt field emission scanning electron microscope (FESEM) to observe PAA, CaO-SiO
2/ PAA and CaO-SiO
2-Ag
2The surface topography of O/PAA and Cross Section Morphology, and analyze this three kinds of elementary composition variations of material surface with X-Ray Energy Spectrum Analysis method (EDS), its result such as Fig. 1～shown in Figure 6.
2.2CaO-SiO
2-Ag
2The simulated body fluid of O/PAA (SBF) immersion test
By the CaO-SiO for preparing
2-Ag
2O/PAA carries out SBF and soaked 1～14 day, adopts FESEM to observe the variation of soaking a period of time rear surface pattern, judgement CaO-SiO
2-Ag
2Whether the O/PAA surface has osteoid apatite to form, and estimates its biological activity.The addition of SBF and CaO-SiO
2-Ag
2The ratio of the surface area of O/PAA is 10mL/cm
2.The CaO-SiO of different time sections will have been soaked
2-Ag
2O/PAA, from SBF, taking out, cleans its surface gently with deionized water, after natural drying 24h, with FESEM, observes the variation of its pattern.Simulated body fluid contains the ion concentration close with human plasma and ion cluster concentration.It consists of:
The experimental result that SBF soaks shows: CaO-SiO
2-Ag
2O/PAA carries out SBF and soaks after 1 day, and the heterogeneous nucleation growth occurs calcium phosphorus crystal on functionalized surface; Along with the prolongation of soak time, the bone like apatite layer that material surface forms is more and more finer and close also to be covered by nano level osteoid apatite gradually; Adopt Fourier transform infrared spectroscopy (FTIR) to analyze CaO-SiO
2-Ag
2O/PAA soaks the variation of different time rear surface chemical composition through SBF, its result has further confirmed CaO-SiO
2-Ag
2What O/PAA formed is osteoid apatite.Explanation thus, the CaO-SiO after year silver
2Still do not affect the ability that it induces the apatite deposition, have good biological activity.In order to study CaO-SiO
2-Ag
2The ion of O/PAA in SBF discharges and degradability, in above-mentioned immersion ratio, sample is placed in to the SBF of 37 ℃ and soaks 6h, 24h, 72h, 120h and 168h, soaks after certain hour ion concentration and the pH value of measuring Ca, Si, P and Ag in SBF solution.Its result as shown in figure 10.2.3. In Vitro Bacteriostasis performance test
PAA, CaO-SiO
2/ PAA and CaO-SiO
2-Ag
2Following method is adopted in the test of O/PAA anti-microbial property-and experimental strain adopts escherichia coli and staphylococcus aureus, get in the LB fluid medium that a certain amount of strain is inoculated in 50ml, 160rpm cultivates 18～24h on the constant-temperature table of 37 ℃, in order to antibacterial test.Equipment used in laboratory sample and test is through 121 ℃ of high temperature sterilize 20min; The bacteria suspension that takes out 1ml bacterium liquid after constant-temperature table is cultivated is in the centrifuge tube that the 9ml normal saline is housed, and concussion evenly, then gets that wherein 1ml is in the centrifuge tube of another 9ml normal saline, and the bacterium liquid after former cultivation is diluted 10
2Doubly; Get bacterium liquid 100 μ l after dilution and be inoculated in and be equipped with in the vial of three kinds of materials that specification is 10mm * 10mm, with the method for piping and druming, make bacterium liquid be uniformly distributed in the surface of material, wherein the PAA sample is blank; Sample and bacterium liquid act on 18～24h in the constant incubator of 37 ℃ after, add respectively the physiological saline solution of 10ml, cover tightly bottle cap, with hand rolling 30s, antibacterial is eluted; Bacterium liquid after eluting is coated with dull and stereotyped with after the suitable multiple of normal saline dilution, getting 100 μ l on the LB solid medium, three of parallel paintings, after in the constant incubator of 37 ℃, cultivating 18～24h, count and average, then convert and obtain the bacteria living number in vial.Be calculated as follows the bacteriostasis rate of antibiotic sample:
In formula:
C
t-the meansigma methods of the bacterial population that records after 18～24h is inoculated and cultivated to control sample;
T
t-the meansigma methods that the bacterial population that records after 18～24h was inoculated and cultivated to the experiment sample.
Beneficial effect
(1) of the present invention simple for process, with low cost and be convenient to promote;
Therefore (2) in the present invention, being written into of silver ion is before forming materials, by sol-gal process, silver ion is uniformly distributed in bioactive materials, can be along with the stripping of bioactive materials slowly delivery of antimicrobials, CaO-SiO simultaneously
2-Ag
2O is evenly distributed in the duct of PAA, increased homogeneity and stability that Ca, Si and Ag ion discharge;
(3) the prepared CaO-SiO of the present invention
2-Ag
2The O/PAA antimicrobial efficiency is high, to the bacteriostasis rate of escherichia coli and staphylococcus aureus all more than 90%.
The accompanying drawing explanation
Fig. 1 is the surface (a) of PAA thin film and the FESEM figure of cross section (b);
Fig. 2 is CaO-SiO
2The FESEM figure of the surface of/PAA (a) and cross section (b);
Fig. 3 is CaO-SiO
2-Ag
2The FESEM figure of the surface of O/PAA (a) and cross section (b);
Fig. 4 is the EDS energy spectrogram of PAA;
Fig. 5 is CaO-SiO
2The EDS energy spectrogram of/PAA;
Fig. 6 is CaO-SiO
2-Ag
2The EDS energy spectrogram of O/PAA;
Fig. 7 is CaO-SiO
2-Ag
2O/PAA soaks the FESEM figure after SBF1 days (a), 3 days (b), 7 days (c) and 14 days (d);
Fig. 8 is CaO-SiO
2-Ag
2O/PAA soaks the surperficial EDS energy spectrogram after SBF1 days (a), 3 days (b), 7 days (c) and 14 days (d);
Fig. 9 is CaO-SiO
2-Ag
2O/PAA soaks the FTIR collection of illustrative plates after SBF1 days, 3 days, 7 and 14 days;
Figure 10 is CaO-SiO
2-Ag
2O/PAA soaks after different time Ca, Si, P and Ag ion concentration and pH variation diagram in SBF solution;
Figure 11 is PAA (a1), CaO-SiO
2/ PAA (b1), CaO-SiO
2-Ag
2The Chinese People's Anti-Japanese Military and Political College enterobacteria of O/PAA (c1) and PAA (a2), CaO-SiO
2/ PAA (b2), CaO-SiO
2-Ag
2The anti-Staphylococcus aureus identification result figure of O/PAA (c2).
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only are not used in and limit the scope of the invention be used to the present invention is described.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
(1) SiO
2The preparation of colloidal sol: the deionized water of 207.4ml is mixed with the nitric acid 3.2ml of 2mol/L, stir and to make it even, then while stirring to the aqueous solution that drips the nitric acid acidify in the ethyl orthosilicate of 21.3ml, along with continuing of stirring carries out, finally form SiO
2Colloidal sol; (volume ratio of ethyl orthosilicate, deionized water and nitric acid is 1:9.73:0.15)
(2) 0.1mol/L silver nitrate solution 21.9ml is mixed with the calcium nitrate solution 60ml of 2mol/L, and stirring makes it to mix;
(3) by the SiO in (1)
2Colloidal sol slowly is added drop-wise in the nitrate mixed solution of silver, calcium, and lasting magnetic agitation makes it fully react 60min, and reaction temperature is controlled at 25 ℃; (CaO-SiO
2-Ag
2The mass percent of O tri-components is 53:45:2);
(4) by aperture, be that the PAA thin film of 200nm is placed in the colloidal sol of above-mentioned preparation and goes to vacuum drying oven, under the relative vacuum degree of 0.09MPa, remain 3h;
(5) take out the gained sample, with ethanol and deionized water, repeatedly clean after several dry 8h under 65 ℃;
(6) under nitrogen atmosphere, by sample 500 ℃ of roastings in Muffle furnace, heating rate is 2 ℃/min, is incubated 5h under 500 ℃;
(7) by CaO-SiO
2/ PAA and CaO-SiO
2-Ag
2O/PAA characterizes with FESEM, EDS respectively, as Fig. 2～shown in Figure 6;
(8) by CaO-SiO
2-Ag
2O/PAA soaks and with FESEM, EDS and FTIR, characterized afterwards in 1 day, 3 days, 7 days and 14 days in SBF, its result such as Fig. 7～and shown in Figure 9.
(1) SiO
2The preparation of colloidal sol: the deionized water of 128.8ml is mixed with the nitric acid 3.2ml of 2mol/L, stir and to make it even, then, while stirring to the aqueous solution that drips the nitric acid acidify in the methyl silicate of 16.1ml, along with continuing of stirring, finally form SiO
2Colloidal sol; (volume ratio of methyl silicate, deionized water and nitric acid is 1:8:0.2)
(2) 0.05mol/L silver nitrate solution 64.4ml is mixed with the calcium nitrate solution 25ml of 4mol/L, and stirring makes it to mix; (3) by the SiO in (1)
2Colloidal sol slowly is added drop-wise in the nitrate mixed solution of silver, calcium, and lasting magnetic agitation makes it fully react 45min, and reaction temperature is controlled at 30 ℃; (CaO-SiO
2-Ag
2The mass percent of O tri-components is 53:45:2)
(4) by aperture, be that the PAA thin film of 150nm is placed in the colloidal sol of above-mentioned preparation and goes to vacuum drying oven, under the relative vacuum degree of 0.085MPa, remain 2.5h;
(5) take out the gained sample, with ethanol and deionized water, repeatedly clean after several dry 8h under 80 ℃;
(6) under argon atmosphere, by sample 500 ℃ of roastings in Muffle furnace, heating rate is 1 ℃/min, is incubated 6h under 450 ℃;
(7) by CaO-SiO
2-Ag
2O/PAA soaks 6h, 24h, 72h, 120h and 168h ion release and the pH variation for research material in SBF, its result as shown in figure 10.
(1) SiO
2The preparation of colloidal sol: the deionized water of 370.2ml is mixed with the nitric acid 7.7ml of 2mol/L, stir and to make it even, then while stirring to the aqueous solution that drips the nitric acid acidify in the ethyl orthosilicate of 30.9ml, along with continuing of stirring carries out, finally form SiO
2Colloidal sol; (volume ratio of ethyl orthosilicate, deionized water and nitric acid is 1:12:0.25)
(2) 0.2mol/L silver nitrate solution 7.0ml is mixed with the calcium nitrate solution 56ml of 2.5mol/L, and stirring makes it to mix;
(3) by the SiO in (1)
2Colloidal sol slowly is added drop-wise in the nitrate mixed solution of silver, calcium, and lasting magnetic agitation makes it fully react 50min, and reaction temperature is controlled at 35 ℃; (CaO-SiO
2-Ag
2The mass percent of O tri-components is 48:51:1);
(4) by aperture, be that the PAA thin film of 250nm is placed in the colloidal sol of above-mentioned preparation and goes to vacuum drying oven, under the relative vacuum degree of 0.1MPa, remain 4h;
(5) take out the gained sample, with ethanol and deionized water, repeatedly clean after several dry 10h under 110 ℃;
(6) under nitrogen atmosphere, by sample 550 ℃ of lower roastings in Muffle furnace, heating rate is 3 ℃/min, is incubated 3h under 550 ℃;
(7) by PAA, CaO-SiO
2/ PAA, CaO-SiO
2-Ag
2O/PAA is for escherichia coli and staphylococcus aureus antibacterial experiment, and its result is as shown in Figure 11 and table 1.
The bacteriostasis property of table 1 anti-biotic material after to antibacterial culturing 24h relatively
Claims (8)
1. CaO-SiO
2-Ag
2The preparation method of O/PAA compound bio membrane material comprises:
(1) nitric acid dropping is entered in deionized water, stir, obtain the aqueous solution of nitric acid acidify, then, while stirring to dripping the aqueous solution of nitric acid acidify in the silicon source, finally obtain SiO
2Colloidal sol; Wherein the volume ratio of silicon source, deionized water and nitric acid is 1:(8～12): (0.15～0.30);
(2) silver nitrate solution is added in calcium nitrate solution, mix homogeneously, obtain the nitrate mixed solution of silver, calcium; Then by SiO
2Colloidal sol is added drop-wise in the nitrate mixed solution of silver, calcium, and under 20-40 ℃ of condition, stirring reaction 40-65min, obtain CaO-SiO
2-Ag
2O colloidal sol; CaO-SiO wherein
2-Ag
2In O, the mass percent of three components is (45～55): (44.5～52): (0.5～3);
(3) the PAA thin film is placed in to above-mentioned CaO-SiO
2-Ag
2In O colloidal sol, then, under 0.085MPa～0.1MPa condition, keep 2-4h, then take out, washing, drying, then roasting under protective gas atmosphere, obtain CaO-SiO
2-Ag
2O/PAA compound bio membrane material.
2. a kind of CaO-SiO according to claim 1
2-Ag
2The preparation method of O/PAA compound bio membrane material is characterized in that: in described step (1), the silicon source is ethyl orthosilicate, methyl silicate or butyl silicate.
3. a kind of CaO-SiO according to claim 1
2-Ag
2The preparation method of O/PAA compound bio membrane material is characterized in that: in described step (2), the concentration of silver nitrate solution is 0.05mol/L-0.2mol/L, and the concentration of calcium nitrate solution is 2mol/L-3mol/L.
4. a kind of CaO-SiO according to claim 1
2-Ag
2The preparation method of O/PAA compound bio membrane material is characterized in that: in described step (3), the aperture of PAA thin film is 100nm-250nm.
5. a kind of CaO-SiO according to claim 1
2-Ag
2The preparation method of O/PAA compound bio membrane material is characterized in that: in described step (3), washing is for using ethanol and deionized water wash 3-5 time.
6. a kind of CaO-SiO according to claim 1
2-Ag
2The preparation method of O/PAA compound bio membrane material is characterized in that: in described step (3), baking temperature is 65 ℃-130 ℃, and be 5-12h drying time.
7. a kind of CaO-SiO according to claim 1
2-Ag
2The preparation method of O/PAA compound bio membrane material is characterized in that: in described step (3), protective gas is nitrogen or argon.
8. a kind of CaO-SiO according to claim 1
2-Ag
2The preparation method of O/PAA compound bio membrane material is characterized in that: roasting is roasting in Muffle furnace in described step (3), and the heating rate of Muffle furnace is 1 ℃/min-4 ℃/min, and sintering temperature is 400 ℃-550 ℃, and roasting time is 3-6h.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104649283A (en) * | 2015-01-21 | 2015-05-27 | 东华大学 | Preparation method for nano beta-calcium silicate/selenium biological active composite material |
| CN105220203A (en) * | 2015-10-29 | 2016-01-06 | 东华大学 | A kind of 316L stainless steel surface nano SiO 2the preparation method of dot matrix |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793949A (en) * | 2012-08-23 | 2012-11-28 | 东华大学 | Preparation method for CaO-SiO2/PAA composite film material having biological activity |
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2013
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793949A (en) * | 2012-08-23 | 2012-11-28 | 东华大学 | Preparation method for CaO-SiO2/PAA composite film material having biological activity |
Non-Patent Citations (1)
| Title |
|---|
| MARIA BELLANTONE ET AL.: "Bacteriostatic action of a novel four-component bioactive glass", 《JOURNAL OF BIOMEDICAL MATERIALS RESEARCH》, vol. 51, no. 3, 28 June 2000 (2000-06-28), pages 484 - 490, XP009016783, DOI: doi:10.1002/1097-4636(20000905)51:3<484::AID-JBM24>3.0.CO;2-4 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104649283A (en) * | 2015-01-21 | 2015-05-27 | 东华大学 | Preparation method for nano beta-calcium silicate/selenium biological active composite material |
| CN104649283B (en) * | 2015-01-21 | 2016-08-03 | 东华大学 | A kind of preparation method of nanometer β-calcium silicates/selenium bioactive composite material |
| CN105220203A (en) * | 2015-10-29 | 2016-01-06 | 东华大学 | A kind of 316L stainless steel surface nano SiO 2the preparation method of dot matrix |
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Application publication date: 20131120 |