CN103394121B - Preparation method for porous composite support material - Google Patents
Preparation method for porous composite support material Download PDFInfo
- Publication number
- CN103394121B CN103394121B CN201310327368.4A CN201310327368A CN103394121B CN 103394121 B CN103394121 B CN 103394121B CN 201310327368 A CN201310327368 A CN 201310327368A CN 103394121 B CN103394121 B CN 103394121B
- Authority
- CN
- China
- Prior art keywords
- preparation
- support frame
- frame material
- support material
- porous composite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000000463 material Substances 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 239000002131 composite material Substances 0.000 title claims abstract description 12
- 229920001661 Chitosan Polymers 0.000 claims abstract description 8
- 229920002261 Corn starch Polymers 0.000 claims abstract description 6
- 239000008120 corn starch Substances 0.000 claims abstract description 6
- 239000012153 distilled water Substances 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000003960 organic solvent Substances 0.000 claims abstract description 5
- 150000001875 compounds Chemical class 0.000 claims description 21
- 239000000725 suspension Substances 0.000 claims description 10
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 230000002706 hydrostatic effect Effects 0.000 claims description 7
- 239000003361 porogen Substances 0.000 claims description 7
- 238000007654 immersion Methods 0.000 claims description 5
- 210000000988 bone and bone Anatomy 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 4
- 230000006835 compression Effects 0.000 abstract description 3
- 238000007906 compression Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract 3
- 239000011148 porous material Substances 0.000 abstract 2
- 238000001035 drying Methods 0.000 abstract 1
- 239000004088 foaming agent Substances 0.000 abstract 1
- 238000000462 isostatic pressing Methods 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 210000001612 chondrocyte Anatomy 0.000 description 1
- 210000004292 cytoskeleton Anatomy 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000963 osteoblast Anatomy 0.000 description 1
- 210000004409 osteocyte Anatomy 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Materials For Medical Uses (AREA)
- Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)
Abstract
The invention discloses a preparation method for a porous composite support material. The preparation method comprises the following steps of: adding a polycrylactone-chitosan composite material into a pore-foaming agent, slowly stirring, then adding corn starch, and uniformly blending to obtain a turbid liquid; and transferring the turbid liquid in a die, performing isostatic pressing, then fixing the turbid liquid taken out of the die in an organic solvent, drying, and then soaking in distilled water to obtain the porous composite support material. Via the mode, the porous composite support material prepared by the preparation method disclosed by the invention has pores with the diameter which is same as the pore diameter of bone tissue engineering, the compression modulus of the material can increase to 23.5MPa, and the interface bonding of the material is good. The preparation method is simple to operate, adopts the raw materials with low price, generates few harmful substances during a preparation process, and can be popularized and applied in a large scale.
Description
Technical field
The present invention relates to field of compound material, particularly relate to a kind of preparation method of porous compound support frame material.
Background technology
Bone tissue engineer refer to will be separated autologous high concentration osteoblast, bone marrow stroma stem cell or chondrocyte, plant after cultured and amplified in vitro in a kind of natural or synthetic, have good biocompatibility, can by human body progressively degraded and absorbed cytoskeleton or claim on extracellular matrix.This biomaterial scaffolds can be the three dimensions that cell provides existence, be conducive to cell and obtain enough nutrient substance, carry out gas exchange, get rid of waste material, cell is grown on the three-dimensional rack of prefabricated form, then by this cell hybrid material implantable bone defect, while biomaterial is progressively degraded, the osteocyte of plantation is constantly bred, thus reaches the object of repairing osseous tissue defect.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of preparation method of porous compound support frame material, the porous compound support frame material function admirable that the method is obtained.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: the preparation method providing a kind of porous compound support frame material, and comprising step is:
(1) poly-second lactone-Chitosan Composites is joined in porogen, and slowly stir, then add corn starch, be in harmonious proportion evenly and obtain suspension;
(2) suspension is transferred to hydrostatic profile in mould, be put in organic solvent fixing after taking-up, dry and obtain porous compound support frame material with distilled water immersion again.
In a preferred embodiment of the present invention, described in step (1), the mass ratio of poly-second lactone-Chitosan Composites and described porogen is 1:1-4.
In a preferred embodiment of the present invention, the time of hydrostatic profile described in step (2) is 10-15 minute.
In a preferred embodiment of the present invention, described in step (2), organic solvent is ethanol, methanol or ethyl acetate.
The invention has the beneficial effects as follows: the preparation method of porous compound support frame material of the present invention, obtained porous compound support frame material has and the hole of bone tissue engineer with aperture, resistance to compression mould can bring up to 23.5MPa, the interface cohesion of material is good, this preparation method is simple to operate, the cost of material used is cheap, and the harmful substance produced in preparation process is few, can apply on a large scale.
Detailed description of the invention
Be clearly and completely described to the technical scheme in the embodiment of the present invention below, obviously, described embodiment is only a part of embodiment of the present invention, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making other embodiments all obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment one:
There is provided a kind of preparation method of porous compound support frame material, comprising step is:
(1) 20g is gathered second lactone-Chitosan Composites to join in 40g porogen, and slowly stir, then add corn starch, be in harmonious proportion evenly and obtain suspension;
(2) suspension to be transferred in mould hydrostatic profile 12 minutes, be put in ethanol fixing after taking-up, dry and obtain porous compound support frame material with distilled water immersion again.
Embodiment two:
There is provided a kind of preparation method of porous compound support frame material, comprising step is:
(1) 15g is gathered second lactone-Chitosan Composites to join in 45g porogen, and slowly stir, then add corn starch, be in harmonious proportion evenly and obtain suspension;
(2) suspension to be transferred in mould hydrostatic profile 14 minutes, be put in ethyl acetate fixing after taking-up, dry and obtain porous compound support frame material with distilled water immersion again.
Embodiment three:
There is provided a kind of preparation method of porous compound support frame material, comprising step is:
(1) 5g is gathered second lactone-Chitosan Composites to join in 20g porogen, and slowly stir, then add corn starch, be in harmonious proportion evenly and obtain suspension;
(2) suspension to be transferred in mould hydrostatic profile 10 minutes, be put in methanol fixing after taking-up, dry and obtain porous compound support frame material with distilled water immersion again.
The beneficial effect of the preparation method of porous compound support frame material of the present invention is: obtained porous compound support frame material has and the hole of bone tissue engineer with aperture, resistance to compression mould can bring up to 23.5MPa, the interface cohesion of material is good, this preparation method is simple to operate, the cost of material used is cheap, the harmful substance produced in preparation process is few, can apply on a large scale.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical field, be all in like manner included in scope of patent protection of the present invention.
Claims (3)
1. a preparation method for porous compound support frame material, is characterized in that, comprises step to be:
(1) poly-second lactone-Chitosan Composites is joined in porogen, and slowly stir, add corn starch again, be in harmonious proportion evenly and obtain suspension, the mass ratio of wherein said poly-second lactone-Chitosan Composites and described porogen is 1: 1-4;
(2) suspension is transferred to hydrostatic profile in mould, be put in organic solvent fixing after taking-up, dry and obtain porous compound support frame material with distilled water immersion again.
2. the preparation method of porous compound support frame material according to claim 1, is characterized in that, the time of hydrostatic profile described in step (2) is 10-15 minute.
3. the preparation method of porous compound support frame material according to claim 1, is characterized in that, described in step (2), organic solvent is ethanol, methanol or ethyl acetate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310327368.4A CN103394121B (en) | 2013-07-31 | 2013-07-31 | Preparation method for porous composite support material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310327368.4A CN103394121B (en) | 2013-07-31 | 2013-07-31 | Preparation method for porous composite support material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103394121A CN103394121A (en) | 2013-11-20 |
| CN103394121B true CN103394121B (en) | 2015-01-28 |
Family
ID=49557962
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310327368.4A Expired - Fee Related CN103394121B (en) | 2013-07-31 | 2013-07-31 | Preparation method for porous composite support material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103394121B (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7368124B2 (en) * | 2003-03-07 | 2008-05-06 | Depuy Mitek, Inc. | Method of preparation of bioabsorbable porous reinforced tissue implants and implants thereof |
| CN100431623C (en) * | 2006-10-23 | 2008-11-12 | 天津大学 | Method for preparing 3D porous bracket of chitosan - copolymer of poly lactic acid |
| CN102423272B (en) * | 2011-09-20 | 2016-03-30 | 复旦大学 | A kind of porous support with network channel and preparation method thereof |
-
2013
- 2013-07-31 CN CN201310327368.4A patent/CN103394121B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN103394121A (en) | 2013-11-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2015101219A4 (en) | Method for preparing cell-laden 3D scaffolds and application | |
| CN101502672B (en) | Method for preparing hydroxyapatite/silk fibroin compound porous stand material | |
| CN105268026B (en) | A kind of preparation method of chitosan/oxidized graphene/nanometer hydroxyapatite three-dimensional stephanoporate compound stent material | |
| CN101897994A (en) | Biological composite scaffold for repairing bone defect and preparation method thereof | |
| CN103143059A (en) | Nano-composite bone defect repair support with multistage pore diameter structure | |
| CN101015712A (en) | Process for preparation of polycaprolactone-chitosan network/hydroxyapatite porous compound support frame material | |
| CN101716382B (en) | Preparation method of trinary composite stent of plasmid DNA / fibrin gel / polymer | |
| CN105521525A (en) | Porous composite scaffold for bone tissue engineering and preparation method therefor | |
| CN102580148A (en) | Preparation method of composite stent for repairing bone defect | |
| CN105963789B (en) | A kind of preparation method of bone tissue engineering stent material | |
| CN114045257A (en) | Vascularized osteoblast membrane scaffold complex and preparation thereof | |
| CN103394121B (en) | Preparation method for porous composite support material | |
| CN103418027A (en) | Preparation method of composite porous scaffold material | |
| CN102580145B (en) | Method for preparing phosphomycin calcium/hexagonal mesoporous silicon/poly glycolide-co-lactide (PLGA) bone tissue stent | |
| CN102580162A (en) | Method for preparing hydroxyapatite/poly glycolide-co-lactide (PLGA)/chitosan three-dimensional porous stent | |
| CN106552286A (en) | The preparation method of artificial cartilage | |
| CN103055347B (en) | Preparation method of cellulose gel material of onion structure | |
| CN102284085A (en) | Preparation method of hexagonal mesoporous silica enhanced polyglycolide-lactide microspherical stent | |
| CN103691002A (en) | Bacterial cellulose/collagen/hydroxyapatite composite material, and preparation and application thereof | |
| CN102319451A (en) | Preparation method of nanometer calcium phosphate enhanced polyglycolide-lactide micrballon support | |
| CN107298821A (en) | A kind of preparation method of highly hygroscopic speed hygroscopic material | |
| JP5360869B2 (en) | Method for producing cultured skin and cultured skin having elastic fiber tissue layer | |
| CN106620878B (en) | Collagen-chitosan composite material capable of slowly releasing icariin and preparation method thereof | |
| CN104606717A (en) | Preparation method for porous beta-TCP ceramic/gelatin composite material | |
| CN106512099A (en) | Chitosan-based bionic bone tissue engineering scaffold and production method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: SUZHOU XIANGCHENG DITRICT MINGDA COMPOSITE MATERIA Free format text: FORMER OWNER: SUZHOU NAAI PURIFICATION TECHNOLOGY CO., LTD. Effective date: 20141209 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 215000 SUZHOU, JIANGSU PROVINCE TO: 215138 SUZHOU, JIANGSU PROVINCE |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20141209 Address after: 215138 Xiangcheng District, Jiangsu Yangcheng Lake Town (Hunan City) Hunan Road Applicant after: Xiangcheng District, Suzhou City sensible composite material factory Address before: Linquan Street Industrial Park of Suzhou city in Jiangsu province 215000 No. 399 Building 2 room 210 Applicant before: Suzhou Naai Purification Technology Co., Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20131120 Assignee: Suzhou Naai Purification Technology Co., Ltd. Assignor: Xiangcheng District, Suzhou City sensible composite material factory Contract record no.: 2015320010155 Denomination of invention: Preparation method for porous composite support material Granted publication date: 20150128 License type: Exclusive License Record date: 20151113 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150128 Termination date: 20160731 |