CN103393723A - Preparation method for hydrolysis extract of pearl powder - Google Patents
Preparation method for hydrolysis extract of pearl powder Download PDFInfo
- Publication number
- CN103393723A CN103393723A CN2013103436037A CN201310343603A CN103393723A CN 103393723 A CN103393723 A CN 103393723A CN 2013103436037 A CN2013103436037 A CN 2013103436037A CN 201310343603 A CN201310343603 A CN 201310343603A CN 103393723 A CN103393723 A CN 103393723A
- Authority
- CN
- China
- Prior art keywords
- preparation
- margarita
- margarita powder
- hydrolyzed extract
- 10min
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000843 powder Substances 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 230000007062 hydrolysis Effects 0.000 title abstract description 7
- 238000006460 hydrolysis reaction Methods 0.000 title abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 235000005956 Cosmos caudatus Nutrition 0.000 claims description 30
- 244000293323 Cosmos caudatus Species 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000000354 decomposition reaction Methods 0.000 claims description 5
- 230000008020 evaporation Effects 0.000 claims description 5
- 238000001704 evaporation Methods 0.000 claims description 5
- 230000003301 hydrolyzing effect Effects 0.000 claims description 5
- 238000009413 insulation Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 238000012958 reprocessing Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 239000013589 supplement Substances 0.000 claims description 5
- 239000008215 water for injection Substances 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 7
- 102000004169 proteins and genes Human genes 0.000 abstract description 7
- 238000010438 heat treatment Methods 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract 1
- 230000000717 retained effect Effects 0.000 abstract 1
- 238000009210 therapy by ultrasound Methods 0.000 abstract 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000001117 sulphuric acid Substances 0.000 description 2
- 235000011149 sulphuric acid Nutrition 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical class NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000003889 eye drop Substances 0.000 description 1
- 229940012356 eye drops Drugs 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 description 1
- 239000001095 magnesium carbonate Substances 0.000 description 1
- 229910000021 magnesium carbonate Inorganic materials 0.000 description 1
- 238000005649 metathesis reaction Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 108090000765 processed proteins & peptides Chemical class 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Medicinal Preparation (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a preparation method for a hydrolysis extract of pearl powder. Pearl liquid with high content of protein and considerable retained natural components can be prepared through heating hydrolysis, intermittent ultrasonic treatment and the like. The preparation method is simple and suitable for industrial production.
Description
Technical field
The present invention relates to field of pharmaceutical preparations, particularly a kind of preparation method of Margarita powder hydrolyzed extract.
Background technology
Margarita is a kind of famous and precious Chinese crude drug, and important effect is being arranged aspect medicinal and skin protection.The main component of Margarita is calcium carbonate and magnesium carbonate, and its content, more than 90%, also contains taurine, peptide class, 17 seed amino acids, 30 various trace elements and abundant vitamin in addition.
The method of producing at present Margarita liquid is mainly acid hydrolysis and enzyme hydrolysis, but both exists protein recovery not high, and mineral salt such as can't utilize at the shortcoming.In patent CN88105369A, first organic acid and Margarita powder are carried out to metathesis reaction, again from obtaining suspension, separating cotton-shaped Margarita keratoprotein, to comprise impurity more due to obtaining albumen, adopting sulphuric acid to wash is hydrolyzed, the hydrolysis after in and excess sulfuric acid, use activated carbon decolorizing, need five steps, protein yield is only 50%; CN1083362A discloses a kind of technique of directly sulphuric acid and Margarita powder being reacted, and protein yield can reach 80%, but wherein mineral salt can't utilize.CN1083363A discloses a kind of enzymatic hydrolysis process, and reaction is difficult to thoroughly, and protein yield also only has 50%, and mineral salt can't utilize.Based on the deficiency that present Margarita extracting solution preparation technique exists, develop that a kind of technique is simple and protein yield is high, the method that keeps more natural component seems very necessary.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, provides a kind of hydrolysis efficiency high, improves simultaneously the preparation method of the Margarita powder hydrolyzed extract of pearl protein content.
For achieving the above object, the invention provides a kind of preparation method of Margarita powder hydrolyzed extract, it is characterized in that comprising the following steps:
(1) take the 10kg Margarita powder and add hydrolytic decomposition pot, add 80kg water for injection, stir 10min;
(2) be heated to 98 ℃, insulation 2h, adopt batch (-type) supersound process 10min simultaneously, 30min intermittently, reprocessing 2～5 times;
(3) stop ultrasonicly, standingly be cooled to 60 ℃ of left and right, supplement the water yield of evaporation to 80L;
(4) the sheet frame filter through 0.45 μ m microporous filter membrane filters, and concentrated 2h to 20kg, obtain Margarita liquid.
Preferably, described in step (2), heating-up temperature is 98 ℃.
Preferably, described in step (2), ultrasonic time is 10min.
Preferably, ultrasonic intermittence described in step (2), number of times was 3 times.
Preferably, described in step (4), the Margarita liquid protein content is more than 2.0mg/mL.
The invention provides a kind of preparation method of Margarita powder hydrolyzed extract, have the following advantages:
(1) by heating hydrolysis, intermittently the physical method such as ultrasonic is processed the Margarita hydrolyzed solution that can obtain high protein content, technique is simple, is suitable for suitability for industrialized production;
(2) can make Margarita liquid obtain the high protein yield, keep more natural component, pearl protein content reaches 2.0mg/mL;
(3) the Margarita liquid protein content that makes is high, can reach 2.0mg/mL, higher than the 1.5mg/mL of prior art, for the production of pearl eye drops, can reduce production costs.
The specific embodiment
The concrete technical scheme of implementing of the present invention is:
Embodiment 1
Take the 10kg Margarita powder and add hydrolytic decomposition pot, add 80kg water for injection, stir 10min; Be heated to 98 ℃, insulation 2h, adopt batch (-type) supersound process 10min simultaneously, 30min intermittently, reprocessing 2 times; Stop ultrasonicly, standingly be cooled to 60 ℃ of left and right, supplement the water yield of evaporation to 80L; Sheet frame filter through 0.45 μ m microporous filter membrane filters, and concentrated 2h to 20kg, obtain Margarita liquid; The mensuration protein content is 2.1mg/mL.
Embodiment 2
Take the 10kg Margarita powder and add hydrolytic decomposition pot, add 80kg water for injection, stir 10min; Be heated to 98 ℃, insulation 2h, adopt batch (-type) supersound process 10min simultaneously, 30min intermittently, reprocessing 3 times; Stop ultrasonicly, standingly be cooled to 60 ℃ of left and right, supplement the water yield of evaporation to 80L; Sheet frame filter through 0.45 μ m microporous filter membrane filters, and concentrated 2h to 20kg, obtain Margarita liquid; The mensuration protein content is 2.2mg/mL.
Embodiment 3
Take the 10kg Margarita powder and add hydrolytic decomposition pot, add 80kg water for injection, stir 10min; Be heated to 98 ℃, insulation 2h, adopt batch (-type) supersound process 10min simultaneously, 30min intermittently, reprocessing 5 times; Stop ultrasonicly, standingly be cooled to 60 ℃ of left and right, supplement the water yield of evaporation to 80L; Sheet frame filter through 0.45 μ m microporous filter membrane filters, and concentrated 2h to 20kg, obtain Margarita liquid; The mensuration protein content is 2.0mg/mL.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (4)
1. the preparation method of a Margarita powder hydrolyzed extract is characterized in that comprising the following steps:
(1) take the 10kg Margarita powder and add hydrolytic decomposition pot, add 80kg water for injection, stir 10min;
(2) be heated to 98 ℃, insulation 2h, adopt batch (-type) supersound process 10min simultaneously, 30min intermittently, reprocessing 2～5 times;
(3) stop ultrasonicly, standingly be cooled to 60 ℃ of left and right, supplement the water yield of evaporation to 80L;
(4) the sheet frame filter through 0.45 μ m microporous filter membrane filters, and concentrated 2h to 20kg, obtain Margarita liquid.
2. the preparation method of a kind of Margarita powder hydrolyzed extract according to claim 1, is characterized in that described in step (2), heating-up temperature is 98 ℃.
3. the preparation method of a kind of Margarita powder hydrolyzed extract according to claim 1, is characterized in that described in step (2), ultrasonic time is 10min.
4. the preparation method of a kind of Margarita powder hydrolyzed extract according to claim 1, is characterized in that ultrasonic intermittence described in step (2), number of times was 3 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310343603.7A CN103393723B (en) | 2013-08-09 | 2013-08-09 | A kind of preparation method of Margarita powder hydrolyzed extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310343603.7A CN103393723B (en) | 2013-08-09 | 2013-08-09 | A kind of preparation method of Margarita powder hydrolyzed extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103393723A true CN103393723A (en) | 2013-11-20 |
| CN103393723B CN103393723B (en) | 2015-12-09 |
Family
ID=49557578
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310343603.7A Active CN103393723B (en) | 2013-08-09 | 2013-08-09 | A kind of preparation method of Margarita powder hydrolyzed extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103393723B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108014068A (en) * | 2017-12-27 | 2018-05-11 | 广西还珠海洋生物科技有限公司 | A kind of pearl cream |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1131016A (en) * | 1995-12-20 | 1996-09-18 | 复旦大学 | Technique for hydrolization of pearl by physical method |
| CN101695505A (en) * | 2009-10-19 | 2010-04-21 | 海南京润珍珠生物技术股份有限公司 | Preparation method of enzyme hydrolysis pearl powder |
| CN102018727A (en) * | 2009-09-18 | 2011-04-20 | 桂林莱茵生物科技股份有限公司 | Preparation method of pearl hydrolysate |
| CN102113991A (en) * | 2011-01-13 | 2011-07-06 | 浙江长生鸟珍珠生物科技有限公司 | Toning lotion containing pearl water extract and preparation method |
| CN102335199A (en) * | 2011-09-30 | 2012-02-01 | 温州大学 | Microwave-hydrolyzation method of pearl powder |
-
2013
- 2013-08-09 CN CN201310343603.7A patent/CN103393723B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1131016A (en) * | 1995-12-20 | 1996-09-18 | 复旦大学 | Technique for hydrolization of pearl by physical method |
| CN102018727A (en) * | 2009-09-18 | 2011-04-20 | 桂林莱茵生物科技股份有限公司 | Preparation method of pearl hydrolysate |
| CN101695505A (en) * | 2009-10-19 | 2010-04-21 | 海南京润珍珠生物技术股份有限公司 | Preparation method of enzyme hydrolysis pearl powder |
| CN102113991A (en) * | 2011-01-13 | 2011-07-06 | 浙江长生鸟珍珠生物科技有限公司 | Toning lotion containing pearl water extract and preparation method |
| CN102335199A (en) * | 2011-09-30 | 2012-02-01 | 温州大学 | Microwave-hydrolyzation method of pearl powder |
Non-Patent Citations (1)
| Title |
|---|
| 李慧等: "珍珠水解工艺的正交实验及微量元素的测定", 《时珍国医国药》, vol. 17, no. 5, 31 December 2006 (2006-12-31) * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108014068A (en) * | 2017-12-27 | 2018-05-11 | 广西还珠海洋生物科技有限公司 | A kind of pearl cream |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103393723B (en) | 2015-12-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103388017B (en) | Preparation technology of fish skin collagen oligopeptide | |
| CN103911416B (en) | Method for preparing active peptide from scallop skirts | |
| CN103224968B (en) | Method for preparing neohesperidin by enzymic method | |
| CN102154420B (en) | Method for extracting pigskin collagen peptides | |
| CN101884412B (en) | Method for preparing natural compound calcium amino acid chelate | |
| CN103232552A (en) | Method for preparing brown algae fucosan and fucoxanthin in enzymic way | |
| US9440918B2 (en) | Method for purifying (S)-Oxiracetam | |
| CN103539869B (en) | A kind of method improving thick heparin sodium extraction technology enzymolysis efficiency | |
| CN106480145A (en) | A kind of extracting method of small molecule oyster polypeptide | |
| CN103275151B (en) | A kind of process for purification of Matachrom | |
| CN103393723B (en) | A kind of preparation method of Margarita powder hydrolyzed extract | |
| CN101270063A (en) | Method for preparing high purity solid cyanoacetic acid | |
| CN106834399A (en) | A kind of Antihypertensive Peptides from Trachyostracous mussel closed shell flesh | |
| CN103012509B (en) | Method of separating and purifying sucrose-6-acetate mother liquor by salt fractionation | |
| CN113200917B (en) | Purification method of 4-aminoantipyrine | |
| CN104557685A (en) | Method for producing nicotinic acid by using nicotinamide mother solution | |
| CN104119409A (en) | Novel method for extracting stevioside by using stevia rebaudian as raw material | |
| CN101591254B (en) | Method for removing salt in betaine solution | |
| CN103408418A (en) | Preparation and purification method of solid malonic acid | |
| CN104496833A (en) | Gabapentin synthesis technology | |
| CN104649922A (en) | Recrystallization method of L-phenylalanine crude product | |
| CN103980140A (en) | Abalone shell sourced calcium glutamate and preparation method thereof | |
| CN103467324A (en) | Preparation method of N, N-dimethylglycine for industrial production | |
| CN107311912B (en) | Method for extracting 1-deoxynojirimycin from silkworm excrement | |
| CN114507269A (en) | Preparation method of acetyl glycyrrhetinic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 215000 Dongyan Road, Loufeng East District, Suzhou Industrial Park, Jiangsu Province Patentee after: Suzhou Lezhu Pharmaceutical Co.,Ltd. Country or region after: China Address before: No. 56 Dongyan Road, Suzhou Industrial Park, Suzhou City, Jiangsu Province, 215021 Patentee before: SUZHOU INDUSTRIAL PARK TIAN LONG MEDICAL Co.,Ltd. Country or region before: China |
|
| CP03 | Change of name, title or address |