CN103393653A - Application of Sarcaboside B to medicament for treatment of carcinoma of bile duct - Google Patents
Application of Sarcaboside B to medicament for treatment of carcinoma of bile duct Download PDFInfo
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- CN103393653A CN103393653A CN2013102642450A CN201310264245A CN103393653A CN 103393653 A CN103393653 A CN 103393653A CN 2013102642450 A CN2013102642450 A CN 2013102642450A CN 201310264245 A CN201310264245 A CN 201310264245A CN 103393653 A CN103393653 A CN 103393653A
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- sarcaboside
- bile duct
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- carcinoma
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Abstract
The invention discloses application of Sarcaboside B to preparation of medicaments for treatment of human carcinoma of bile duct and belongs to the technical field of novel application of medicaments. Through in vitro MTT antineoplastic activity evaluation, it is discovered that Sarcaboside B has substantial inhibition effect on growth of a cell strain RBE of human carcinoma of bile duct. Accordingly, the Sarcaboside B can be applied to the preparation of medicaments for resisting carcinoma of bile duct and has good development and application prospects. The application of Sarcaboside B to preparation of medicaments for treatment of carcinoma of bile duct involved in the invention is disclosed for the first time. The Sarcaboside B has a novel skeleton type and unimaginably strong activity in inhibition of cells of human carcinoma of bile duct.
Description
Technical field
The present invention relates to the new purposes of compound S arcaboside B, relate in particular to the application of Sarcaboside B in the anti-cancer of biliary duct medicine of preparation.
Technical background
Cancer is to one of disease of human life's health hazard maximum, and annual have a large amount of people to die from cancer.The research and development of cancer therapy drug are the focuses of study of pharmacy always.In antitumor drug, having 74% to be the natural product or derivatives thereof, is exactly the reasonable antitumor drug of effect clinically at present as paclitaxel and derivant thereof.Therefore, from searching anticancer compound or lead compound natural product, have great importance.
the compound S arcaboside B that the present invention relates to is one and delivered (Li in 2012, X. et al., 2012. Two New-Skeleton Compounds from Sarcandra glabra. Helvetica Chimica Acta 95 (6), 998-1002.) New skeleton compound, this compound has brand-new framework types, there is no at present the report about activity, for the purposes in preparation treatment cancer of biliary duct medicine that the present invention relates to, belong to open first, because framework types belongs to brand-new framework types, and its inhibition for cholangiocarcinoma cell is active unexpectedly strong, there do not is the possibility that is provided any enlightenment by other compounds, possesses outstanding substantive distinguishing features, control for cancer of biliary duct simultaneously obviously has significant progress.
Summary of the invention
The invention provides the application of compound S arcaboside B in preparing antitumor drug.
The present invention adopts following technical scheme: the application of Sarcaboside B in the anti-cancer of biliary duct medicine of preparation, and the structural formula of Sarcaboside B is as shown in formula I:
Formula I
The present invention finds by external MTT anti-tumor activity evaluation, and Sarcaboside B also has significant inhibitory action to the growth of human bile duct cancer cell strain RBE, suppresses the IC of this 1 strain Growth of Cells
50Value is 2.23 ± 0.33 μ M.Therefore, Sarcaboside B can, for the preparation of anti-cancer of biliary duct medicine, have good development prospect.
The purposes of the Sarcaboside B that the present invention relates in preparation treatment cancer of biliary duct medicine belongs to open first, because framework types belongs to brand-new framework types, and its inhibition for cholangiocarcinoma cell is active unexpectedly strong, there do not is the possibility that is provided any enlightenment by other compounds, possess outstanding substantive distinguishing features, the control for cancer of biliary duct simultaneously obviously has significant progress.
The present invention is further detailed explanation by the following examples, but protection scope of the present invention is not subjected to any restriction of specific embodiment, but be limited by claim.
The specific embodiment
The preparation method of compound S arcaboside B involved in the present invention is referring to document (Li, X. et al., 2012. Two New-Skeleton Compounds from Sarcandra glabra. Helvetica Chimica Acta 95 (6), 998-1002.).
The present invention is further detailed explanation by the following examples, but protection scope of the present invention is not subjected to any restriction of specific embodiment, but be limited by claim.
Embodiment 1: the preparation of compound S arcaboside B tablet involved in the present invention:
Get 20 and digest compound Sarcaboside B, add conventional adjuvant 180 grams that prepare tablet, mix, conventional tablet machine is made 1000.
Embodiment 2: the preparation of compound S arcaboside B capsule involved in the present invention:
Get 20 and digest compound Sarcaboside B, add the conventional adjuvant such as starch 180 grams that prepare capsule, mix, encapsulatedly make 1000.
Below by pharmacodynamic experiment, further illustrate its pharmaceutically active.
Experimental example: adopt the growth inhibited effect of mtt assay assessing compound Sarcaboside B to the human bile duct cancer cell strain
1. method: the cell that is in the growth logarithmic (log) phase: human bile duct cancer cell strain RBE(buys from Chinese Academy of Sciences's cell bank) with 1.5 * 10
4The concentration kind is in 96 orifice plates.After cell culture 24 h are adherent, suck original culture medium.Test is divided into blank group, drug treating group.Blank group is changed 1640 culture medium that contain 10% hyclone; It is 100 μ M that the replacing of drug treating group contains concentration, 50 μ M, 10 μ M, 1 μ M, 0.1 μ M, the culture medium of the Sarcaboside B of 0.01 μ M and 0.001 μ M.After cultivating 48 h, add the MTT of concentration 5 mg/mL, continue to be put in CO
2Incubator is cultivated 4 h, then along culture fluid top, suck 100 μ L supernatants, add 100 μ L DMSO, 10 min are placed in dark place, utilize microplate reader (Sunrise company product) to measure light absorption value (wavelength 570nm), and calculating the cell survival situation according to light absorption value, 6 repeating holes are established in each processing.Cell survival rate (%)=Δ OD
Drug treating/ Δ OD
Blank* 100.
2. result: Sarcaboside B has significant inhibitory action to the growth of human bile duct cancer cell strain RBE.This compound suppresses the IC of human bile duct cancer cell strain RBE growth
50Value is: 2.23 ± 0.33 μ M.
By above-described embodiment, shown, Sarcaboside B of the present invention has good inhibitory action to the growth of human bile duct cancer cell strain RBE.Prove thus, Sarcaboside B of the present invention has anti-cancer of biliary duct activity, can be for the preparation of anti-cancer of biliary duct medicine.
Claims (1)
1.Sarcaboside the application of B in treatment cancer of biliary duct medicine, described compound S arcaboside B structure as
Formula IShown in:
Formula I.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102642450A CN103393653A (en) | 2013-06-27 | 2013-06-27 | Application of Sarcaboside B to medicament for treatment of carcinoma of bile duct |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102642450A CN103393653A (en) | 2013-06-27 | 2013-06-27 | Application of Sarcaboside B to medicament for treatment of carcinoma of bile duct |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103393653A true CN103393653A (en) | 2013-11-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013102642450A Pending CN103393653A (en) | 2013-06-27 | 2013-06-27 | Application of Sarcaboside B to medicament for treatment of carcinoma of bile duct |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103393653A (en) |
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2013
- 2013-06-27 CN CN2013102642450A patent/CN103393653A/en active Pending
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Application publication date: 20131120 |