CN103389287A - A high resolution optical system suitable for the imaging of a liver surface in vivo - Google Patents
A high resolution optical system suitable for the imaging of a liver surface in vivo Download PDFInfo
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- CN103389287A CN103389287A CN2013102984528A CN201310298452A CN103389287A CN 103389287 A CN103389287 A CN 103389287A CN 2013102984528 A CN2013102984528 A CN 2013102984528A CN 201310298452 A CN201310298452 A CN 201310298452A CN 103389287 A CN103389287 A CN 103389287A
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Abstract
The present invention relates to a high resolution optical system suitable for the imaging of a liver surface in vivo. The optical system comprises a titanium sapphire mode-locked femtosecond laser, an acousto-optic modulator, an optical scanning device, a variable reflecting mirror, a microscope objective, an objective table, a dichroic beamsplitter, a near-infrared filter, a reflection grating, a photomultiplier tube array, a computer control processing unit, a semiconductor laser and an image sensor. The optical system of the invention is ingenious and reasonable in design, and has broad development prospects and relatively great promotion significance.
Description
Technical field
The present invention relates to a kind of high-resolution optics system that is applicable to the surface imaging of live body liver.
Background technology
As everyone knows, the liver surface mainly is comprised of extracellular matrix (collagenous fibres and snapback fibre), liver cell and microcirculqtory system.These compositions have great importance to support structure and the function of liver., when liver is impaired or during pathology, these compositions also corresponding variation can occur, as hyperplasia, liver cell form and the metabolic variation of extracellular matrix and blood capillary form and little blood flow, change dynamically.In addition, because changing, these all occur in micro-meter scale.Therefore can realize information such as these compositions and structures thereof in the extraction of micro-meter scale and visual liver physiological Study and liver disease are diagnosed and had great value.
Yet, current image technology, such as traditional endoscope, x-ray imaging, magnetic resonance imaging, CT scan, ultrasonic imaging, positron emission tomography and optical coherent chromatographic imaging etc., because its precision and resolution can't reach micro-meter scale, thereby can't realize the detection to these variations.
In recent years, the multi-photon imaging technique is owing to can realizing that biological tissue integrates and visual in the information of micro-meter scale, has shown huge potentiality in the application of live body high-resolution imaging.This technology utilizes laser and the second harmonic of organizing inherent interaction between component to occur to produce (Second Harmonic Generation, SHG) and two-photon fluorescence excitation (Two-Photon Excited Fluorescence, the nonlinear optical effect such as TPEF), can realize reduction pyridine nucleotide (the nicotinamide adenine dinucleotide to cell source, NADH) and oxidation flavoprotein (falvin adenine dinucleotide, FAD), the collagenous fibres in extracellular matrix source and the high-resolution imaging of snapback fibre.Simultaneously, the fluorescence intensity ratio of two of cell source different TPEF signal in band (430-490 nm and 500-560 nm) can be used as the Endogenous index of estimating cell metabolism.In addition, laser speckle imaging technology can carry out to the living body biological microcirculation blood flow the real-time whole audience imaging of high-spatial and temporal resolution.Owing to having noncontact, without wound, the advantages such as fast imaging, laser speckle imaging technology is highly suitable for the measurement of blood microcirculation.Use laser speckle technique can measure the microcirculation parameters such as blood vessels caliber, vessel density, velocity of blood flow and blood perfusion.Can infer, laser speckle imaging technology also has very large application potential in the monitoring of the microcirculqtory system on live body liver surface.
Therefore, develop a kind of high-resolution optics system that combines with laser speckle imaging technology based on the multi-photon imaging technique, can for extraction and visual the provide new technology of the information such as live body liver surface principal ingredient and structure thereof at micro-meter scale is provided, liver physiological Study and liver disease diagnosis be had great importance.
Summary of the invention
In view of this, the purpose of this invention is to provide a kind of high-resolution optics system that is applicable to the surface imaging of live body liver.
the present invention adopts following scheme to realize: a kind of high-resolution optics system that is applicable to the surface imaging of live body liver, comprise a titanium jewel locked mode femto-second laser, it is characterized in that: the near infrared ultrashort pulse light that described titanium jewel locked mode femto-second laser inspires carries out power attenuation through an acousto-optic modulator, again through a dichroic beam splitter, the live body liver that arrives on objective table by a microcobjective after one optical scanning device and a variable mirror is surperficial, excite and produce the endogenous non-linearity luminous signs, described light signal is through described microcobjective, variable mirror and optical scanning device arrive a dichroic beam splitter, incide a reflection grating through an isolated light signal of near infrared filter plate again, described reflection grating is separated light signal by different-waveband and the photomultiplier tube array that leads is surveyed, and the signal input one computer control processing unit that detects, realize the imaging of different-waveband endogenous non-linearity luminous signs, then, the live body liver that provides the semiconductor laser instrument to incide on described objective table is surperficial, produce a scattered light signal, described scattered light signal is surveyed through described microcobjective and variable mirror guiding one imageing sensor, and the described computer control processing unit of the signal that will detect input, realize laser speckle blood current imaging.
In an embodiment of the present invention, described semiconductor laser incides on described objective table with 30 ° of levels.
In an embodiment of the present invention, described variable mirror can be realized the switching of catoptron and empty mirror.
In an embodiment of the present invention, described titanium jewel locked mode femto-second laser is the ultrashort pulse laser of high repetition frequency, and frequency reaches 84 MHz, and ultrashort pulse is 10 fs, and wavelength coverage is 730-980 nm, and output power is 1.8 W.
In an embodiment of the present invention, described reflection grating is a high-quality reflection grating, plays a minute light action, can separate light signal by different-waveband interval 6 nm.
In an embodiment of the present invention, described photomultiplier tube array is comprised of 30 photomultipliers, the wavelength coverage 380-560 nm of detection.
In an embodiment of the present invention, described semiconductor laser is mini semiconductor laser, wavelength 650nm, and output power is 30 mW.
Remarkable advantage of the present invention is: utilize the inherent interaction between component in femtosecond laser and liver surface to produce NADH and FAD, the collagenous fibres in extracellular matrix source and the high-resolution imaging of snapback fibre of endogenic nonlinear optics signal to cell source, realize the extraction of the change informations such as 26S Proteasome Structure and Function of hepatocellular form and metabolism, extracellular matrix and visual; The imaging of the scattered light signal that utilizes semiconductor laser to incide liver surface and produce to the liver microcirculqtory system, thus realize the monitoring of the blood capillary form of liver surface and little blood flow multidate information and visual; The optical system that combines with laser speckle imaging technology based on the multi-photon imaging technique provides new method and new technology for the high-resolution imaging of realizing live body liver surface, and liver physiological Study and liver disease diagnosis are had great value.The present invention is reasonable in design, is skillfully constructed, and has vast potential for future development and larger dissemination.
, for making purpose of the present invention, technical scheme and advantage clearer, below will by specific embodiment and relevant drawings, the present invention be described in further detail.
Description of drawings
Fig. 1 is system architecture schematic diagram of the present invention.
Embodiment
The present invention utilizes the inherent interaction between component of femtosecond laser and liver surface to produce NADH and FAD, the collagenous fibres in extracellular matrix source and the high-resolution imaging of snapback fibre of endogenic nonlinear optics signal to cell source, realize the extraction of the change informations such as 26S Proteasome Structure and Function of hepatocellular form and metabolism, extracellular matrix and visual, the imaging of the scattered light signal that then utilizes semiconductor laser to incide liver surface and produce to the liver microcirculqtory system, thus realize the monitoring of the blood capillary form of liver surface and little blood flow multidate information and visual.
as shown in Figure 1, the invention provides a kind of high-resolution optics system that is applicable to the surface imaging of live body liver, comprise a titanium jewel locked mode femto-second laser 1, the near infrared ultrashort pulse light that described titanium jewel locked mode femto-second laser 1 inspires carries out power attenuation through an acousto-optic modulator 2, again through a dichroic beam splitter 7, the live body liver that arrives on objective table 6 by a microcobjective 5 after one optical scanning device 3 and a variable mirror 4 is surperficial, excite and produce the endogenous non-linearity luminous signs, described light signal is through described microcobjective 5, variable mirror 4 and optical scanning device 3 arrive a dichroic beam splitter 7, incide a reflection grating 9 through an isolated light signal of near infrared filter plate 8 again, described reflection grating 9 is separated light signal by different-waveband and the photomultiplier tube array 10 that leads is surveyed, and the signal input one computer control processing unit 15 that detects, realize different-waveband (SHG:390-410 nm, TPEF:430-490 nm and TPEF:500-560 nm) imaging 11 of endogenous non-linearity luminous signs, thereby realize NADH and FAD to cell source, the collagenous fibres in extracellular matrix source and the high-resolution imaging of snapback fibre, can extract liver cell form and metabolism by graphical analysis, the information such as collagenous fibres and snapback fibre fine structure, then, the live body liver that provides semiconductor laser instrument 12 to incide on described objective table 6 is surperficial, produce a scattered light signal, described scattered light signal is through described microcobjective 5 and variable mirror 4 guiding one imageing sensor 13(CCD) survey, and the described computer control processing unit of the signal that will detect input, realize laser speckle blood current imaging 14, can extract the microcirculation information such as blood vessels caliber, vessel density, velocity of blood flow and blood perfusion by graphical analysis.
Preferably, described semiconductor laser incides on described objective table with 30 ° of levels; Described titanium jewel locked mode femto-second laser is the ultrashort pulse laser of high repetition frequency, and frequency reaches 84 MHz, and ultrashort pulse is 10 fs, and wavelength coverage is 730-980 nm, and output power is 1.8 W; Described reflection grating is a high-quality reflection grating, plays a minute light action, can separate light signal by different-waveband interval 6 nm; Described photomultiplier tube array is comprised of 30 photomultipliers, the wavelength coverage 380-560 nm of detection; Described semiconductor laser is mini semiconductor laser, wavelength 650nm, and output power is 30 mW.
it is worth mentioning that, described variable mirror is a mirror with switching effect, it can make light produce reflection or transmission, can realize that catoptron and empty mirror (are diaphotoscope, light can be directly sees through empty mirror and propagates) switching, when near infrared ultrashort pulse light that titanium jewel locked mode femto-second laser sends by after optical scanning device, variable mirror switches to catoptron and is beneficial near infrared ultrashort pulse light and incides in microcobjective, after scattered light signal passes through microcobjective, variable mirror switches to the sky mirror and is beneficial to scattered light signal and incides in imageing sensor.
Above-listed preferred embodiment; the purpose, technical solutions and advantages of the present invention are further described; institute is understood that; the foregoing is only preferred embodiment of the present invention; not in order to limit the present invention; within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (7)
1. high-resolution optics system that is applicable to the imaging of live body liver surface, comprise a titanium jewel locked mode femto-second laser, it is characterized in that: the near infrared ultrashort pulse light that described titanium jewel locked mode femto-second laser inspires carries out power attenuation through an acousto-optic modulator, again through a dichroic beam splitter, the live body liver that arrives on objective table by a microcobjective after one optical scanning device and a variable mirror is surperficial, excite and produce the endogenous non-linearity luminous signs, described light signal is through described microcobjective, variable mirror and optical scanning device arrive a dichroic beam splitter, incide a reflection grating through an isolated light signal of near infrared filter plate again, described reflection grating is separated light signal by different-waveband and the photomultiplier tube array that leads is surveyed, and the signal input one computer control processing unit that detects, realize the imaging of different-waveband endogenous non-linearity luminous signs, then, the live body liver that provides the semiconductor laser instrument to incide on described objective table is surperficial, produce a scattered light signal, described scattered light signal is surveyed through described microcobjective and variable mirror guiding one imageing sensor, and the described computer control processing unit of the signal that will detect input, realize laser speckle blood current imaging.
2. a kind of high-resolution optics system that is applicable to the imaging of live body liver surface according to claim 1, it is characterized in that: described semiconductor laser incides on described objective table with 30 ° of levels.
3. a kind of high-resolution optics system that is applicable to the imaging of live body liver surface according to claim 1, it is characterized in that: described variable mirror can be realized the switching of catoptron and empty mirror.
4. a kind of high-resolution optics system that is applicable to the imaging of live body liver surface according to claim 1, it is characterized in that: described titanium jewel locked mode femto-second laser is the ultrashort pulse laser of high repetition frequency, frequency reaches 84 MHz, ultrashort pulse is 10 fs, wavelength coverage is 730-980 nm, and output power is 1.8 W.
5. a kind of high-resolution optics system that is applicable to the imaging of live body liver surface according to claim 1, it is characterized in that: described reflection grating is a high-quality reflection grating, play a minute light action, can separate light signal by different-waveband interval 6 nm.
6. a kind of high-resolution optics system that is applicable to the imaging of live body liver surface according to claim 1, it is characterized in that: described photomultiplier tube array is comprised of 30 photomultipliers, the wavelength coverage 380-560 nm of detection.
7. a kind of high-resolution optics system that is applicable to the imaging of live body liver surface according to claim 1, it is characterized in that: described semiconductor laser is mini semiconductor laser, wavelength 650nm, output power is 30 mW.
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| CN107110774A (en) * | 2014-12-23 | 2017-08-29 | 马克斯-普朗克科学促进学会 | Method and instrument for measure spectrum sample response |
| CN107688014A (en) * | 2016-09-21 | 2018-02-13 | 北京大学 | A kind of cell imaging method |
| CN108594418A (en) * | 2018-03-29 | 2018-09-28 | 暨南大学 | A kind of light field micro imaging system and its method based on array single pixel detector |
| CN110198786A (en) * | 2016-11-14 | 2019-09-03 | 浩康生物系统公司 | Method and apparatus for sorting target particles |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107688014A (en) * | 2016-09-21 | 2018-02-13 | 北京大学 | A kind of cell imaging method |
| CN110198786A (en) * | 2016-11-14 | 2019-09-03 | 浩康生物系统公司 | Method and apparatus for sorting target particles |
| US11471885B2 (en) | 2016-11-14 | 2022-10-18 | Orca Biosystems, Inc. | Methods and apparatuses for sorting target particles |
| CN108594418A (en) * | 2018-03-29 | 2018-09-28 | 暨南大学 | A kind of light field micro imaging system and its method based on array single pixel detector |
| CN108594418B (en) * | 2018-03-29 | 2021-02-05 | 暨南大学 | Light field microscopic imaging system and method based on array single-pixel detector |
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