CN103374055A - Method for separating and purifying reduced glutathione (GSH) from reduced glutathione contained fermentation leaching liquid - Google Patents
Method for separating and purifying reduced glutathione (GSH) from reduced glutathione contained fermentation leaching liquid Download PDFInfo
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Abstract
The invention provides a method for separating and purifying reduced glutathione (GSH) from a reduced GSH contained fermentation leaching liquid. The method comprises the following steps of: (1) treating the fermentation leaching liquid sequentially through anion exchange resin and a non-polar absorbent to obtain a reduced GSH contained solution; and (2) concentrating the reduced GSH contained solution, and then, crystallizing and drying. The reduced GSH separated and purified by using the method provided by the invention has the advantages that the purity of the reduced GSH can be up to over 98.5% (titration, drying), and relevant impurities conform to the requirement of European pharmacopoeia.
Description
Technical field
The present invention relates to the reduced glutathion separation purification method of (being called for short GSH), relate in particular to a kind of method by resin method separation and purification reduced form GSH from the fermented liquid that contains reduced form GSH.
Background technology
GSH be by L-glutamic acid, halfcystine and glycine by the tripeptide compound that the peptide bond condensation forms, be a kind of broad-spectrum bioactive peptide.The molecular weight of GSH is 307.33, and fusing point is 189～193 ℃ (decomposition), and iso-electric point is 5.93, and it is slender rod shaped that crystal is water white transparency.Its water-soluble, liquefied ammonia is insoluble to alcohol, ether and acetone.The GSH solid is comparatively stable, and its aqueous solution is easily oxidized in air.The reduced form structural formula is
Because GSH is a kind of very special amino acid derivative, is again the tripeptides that contains sulfydryl, so important effect is arranged in vivo.(1) Green Tea Extract, Cell protection.GSH can be used as the substrate of Selenoperoxidase, suppresses lipid peroxidation, and the Cell protection film recovers cell function.(2) remove the exogenous toxic substance toxicity of (comprising medicine).GSH can combine with the toxic compounds that enters body, heavy metal ion or carcinogenic substance etc., and short its excrete, in playing and detoxification.(3) promote the cell synthetic protein.But GSH Cell protection film makes it to exempt from oxidisability and destroys, and prevents the reduction of erythrocyte hemolysis and promotion methemoglobin, and anoxenia, discomfort nauseating and that hepatic diseases causes are had mitigation.(4) participate in transmethylase, turn the third amino reaction, keep the liver cell normal function.(5) participate in bilirubin metabolism, promote the cholic acid metabolism, alleviate bleeding tendency.GSH for radioactive rays, radiopharmaceuticals or since the symptoms such as oligoleukocythemia that antitumor drug causes can play a protective role.
The purification process of GSH mainly contains mantoquita method and resin cation (R.C.) method according to the literature.
Adopting the mantoquita method is the drawback that mainly there is heavy metal contamination in main extraction process and adopts toxic gas hydrogen sulfide, and the difficult problem that also there is cost in electrochemical method and uses.
Chinese patent application 200810233835.5,200610040606.3 disclosed methods are the methods with Zeo-karb purifying GSH, and the resin anion(R.A) that adopts with the present invention has very big-difference, and purity is not as good as the present invention.
Japanese patent application JP20032845 has announced a kind of method for preparing successively Sleep-promoting factor B with polymeric adsorbent and ion exchange resin removal reduced glutathion, the separation method of the Sleep-promoting factor B of announcing is the sp207 resin absorption---after WA30 ion exchange resin and with 2% and 5% acetate gradient desorb---collect the cut of 5% acetic acid desorb concentrated then use again the XAD-4 plastic resin treatment---is concentrated into 40% concentration, 2 ℃, crystallization in 6 hours obtains the oxidized form solid.The gsh that is mainly oxidized form of its preparation, yield is about 58%, not mentioned relevant purity information.
Summary of the invention
Present situation in view of prior art, the method of separation and purification GSH from the GSH fermented liquid that the purpose of this invention is to provide a kind of high-efficiency and low-cost, the inventive method is easy and simple to handle, little, the mild condition of pollution, be convenient to industrial amplification production, adopt the GSH purity of the present invention's preparation high, meet the requirement of European Pharmacopoeia.
Of the present invention from the fermentation extract that contains reduced glutathion the method for separation and purification reduced glutathion may further comprise the steps:
1) described fermentation extract is processed in turn through anionite-exchange resin, non-polar adsorbent, obtained containing the solution of reduced glutathion;
2) concentrated, crystallization, the dry above-mentioned solution that contains reduced glutathion.
Fermentation extract of the present invention is after microbial fermentation finishes, the extract that contains reduced form GSH that obtains by ordinary method.For example, cultivate the yeast that contains reduced form GSH by Chinese patent application 200710036925.1 described methods, after the fermentation ends, adopt conventional solid-liquid separation means separate fermentation liquid, for example behind peracidity hot-water extraction or acid bacterium liquid high pressure broken wall, obtain containing the fermentation extract of reduced form GSH.Preferably, can concentrate the concentrated solution that extract obtains containing reduced form GSH.
According to of the present invention one preferred embodiment, in the described fermentation extract content of reduced glutathion change with the different of amount of water with fermentation level and to some extent difference about 5～10mg/ml.
According to of the present invention one preferred embodiment, above-mentioned steps 1) described in anionite-exchange resin be strongly basic anion exchange resin or weak base anion-exchange resin, more excellent is strongly basic anion exchange resin.
Perhaps, above-mentioned steps 1) anionite-exchange resin described in is macroporous type anionite-exchange resin or gel-type anionite-exchange resin, and more excellent is gel-type anionite-exchange resin.
According to one of the present invention before anion exchange process, be 3.0-5.0 with the pH regulator of described fermentation extract preferred embodiment, preferred 3.5-4.0.
Usually, through behind the anionite-exchange resin, available 2-4 times, the deionization washing resin of preferred 3 times of resin volumes is used 0.05-0.2mol/L again, and the acidolysis of preferred 0.1mol/L is inhaled, and flow velocity is 1-2BV/h.Described acid is selected from sulfuric acid, formic acid or acetic acid.
According to of the present invention one preferred embodiment, above-mentioned steps 1) polar adsorbent described in comprises polystyrene polymeric adsorbent, halogen-containing polystyrene polymeric adsorbent, gac and carbide resin, be preferably polystyrene polymeric adsorbent, halogen-containing polystyrene polymeric adsorbent, more preferably halogen-containing polystyrene polymeric adsorbent.
According to one of the present invention before processing with non-polar adsorbent, be 1.0～4.0 with the pH regulator of described fermentation extract preferred embodiment, preferred 2.5～3.0.
Usually, through behind the nonpolar adsorption resin, with 2-4 doubly, the deionization of preferred 3 times of resin volumes washing resin is used 0.5-5%, preferred 2% aqueous solutions of organic solvent desorb, and flow velocity is less than 1BV/h.Described organic solvent is selected from methyl alcohol, ethanol, Virahol or acetone.
According to a particularly preferred embodiment of the present invention, above-mentioned steps 2) concrete steps are as follows: the fermentation extract is after non-polar adsorbent is processed, obtain containing highly purified reduced form GSH stripping liquid, concentrated this stripping liquid, add crystal seed, add the solvent that dissolves each other with water, such as lower alcohol, acetone etc., solvent-the water mixture that perhaps dissolves each other with water filters after the stirred crystallization, drying obtains the GSH crystal.
According to of the present invention one preferred embodiment, described lower alcohol is methyl alcohol, ethanol or Virahol.According to of the present invention another preferred embodiment, the concentration of solvent is 20-80% (V/V) in solvent-water mixture that described and water dissolve each other, preferred 50%.
The inventive method is easy and simple to handle, little, the mild condition of pollution, is convenient to industrial amplification production.The purity of reduced form GSH after the separation and purification of use the inventive method is more than 98.5% (volumetry is given money as a gift), and related impurities meets the requirement of European Pharmacopoeia.
Embodiment
Below be the detection method of the HPLC of embodiment:
Moving phase:
The A phase: take by weighing SODIUM PHOSPHATE, MONOBASIC 6.8g, sodium heptanesulfonate 2.2g is dissolved in water, and is settled to 1000ml, transfers to pH3.0 with phosphoric acid
B phase: methyl alcohol
A∶B＝97∶3
Chromatographic column: C18 chromatographic column, 4.6 * 250mm, 5 μ m
Chromatographic condition: flow velocity 1ml/min, 30 ℃ of column temperatures detect wavelength 210nm
The titration method of following embodiment:
Take by weighing 0.5g determinand and 2.0g potassiumiodide water-soluble after, be settled to 50ml with volumetric flask, ice bath.The iodine that adds 10ml hydrochloric acid and 20.0ml 0.05mol/L is placed 15min at the lucifuge place.With the Sulfothiorine titration of 0.1mol/L, with the 1ml starch solution as indicator.Do the iodine amount of blank calculation consumption.The iodine of every milliliter of 0.05mol/L is equivalent to the reduced glutathion of 30.73mg.
In the following embodiments, the acquisition that contains reduced form GSH fermented liquid is undertaken by Chinese patent 200710036925.1 described modes, is specially:
Get the bio-reactor that yeast Saccharomyces cerevisiae ATCC7754 wet thallus 2kg places 7.5L, add again 2L tap water, 160g glucose (concentration 4%), 24g soy peptone (concentration 0.6%), 48g ammonium sulfate (concentration 1.2%) and 0.04g copper sulfate (concentration 0.001%), form the 4L fermented liquid, stirring velocity is 300rpm, air flow is 0.5vvm, temperature is controlled at 30 ℃ and cultivates 28h, sampling analysis, the result obtains the GSH fermented liquid of 3.2g/L.Fermented liquid adds the deionized water of 0.5 times of amount (mass volume ratio) by the amount of wet thallus after solid-liquid separation obtains thalline, transfer to pH2 with sulfuric acid, is heated to 80 ℃～90 ℃ and keeps 20min, removes the extract that thalline obtains containing GSH after the cooling.
Embodiment 1
Get GSH extract 2L, contain GSH12.8g.Extract is transferred to pH4.0 with gel type strong base anionite-exchange resin FPA40 purifying, with the deionization washing resin of 3 times of resin volumes, use again the desorb of 0.1mol/L hydrochloric acid, collect concentration and be higher than the stripping liquid of 1mg/ml and be concentrated into 200ml.This concentrated solution is transferred the upper halogen-containing nonpolar adsorption resin SP207 of pH2.8, deionization washing resin with 3 times of resin volumes, aqueous ethanolic solution desorb with 2%, consider the impact of yield and purity, collect reduced glutathion concentration: the ratio of Sleep-promoting factor B concentration (R/O) is greater than 20 stripping liquid and be concentrated into 30ml, add again 15ml ethanol after at room temperature stirring a small amount of crystal seed of adding, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 6 .1g, microscopically is viewed as white or translucent styloid, total recovery 47.6%, and the weight purity 99.62% that records solid with HPLC method (together lower) (is given money as a gift, together lower), titration content 99.66% (giving money as a gift, lower same) meets the requirement of European Pharmacopoeia.
Embodiment 2
Get GSH fermented liquid 2L, contain GSH12.1g.Extract is transferred to pH4.0 with gel type strong base anionite-exchange resin FPA40, with the deionization washing resin of 3 times of resin volumes, use again the desorb of 0.1mol/L hydrochloric acid, collect concentration and be higher than the stripping liquid of 1mg/ml and be concentrated into 200ml.This concentrated solution is transferred the upper halogen-containing nonpolar adsorption resin SP207 of pH2.8, deionization washing resin with 3 times of resin volumes, ethanol desorb with 2%, collect reduced glutathion concentration: the ratio of Sleep-promoting factor B concentration is greater than 20 stripping liquid and be concentrated into 30ml, add again 15ml ethanol after at room temperature stirring a small amount of crystal seed of adding, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 5 .9g, microscopically is viewed as white or translucent styloid, total recovery 68.8%, the weight purity 99.23% of solid, titration content＞99.95%.
Embodiment 3
Get GSH extract 2L, contain GSH12.1g, contain gsh 11.3g.Transfer to pH4.0 with macroporous type strong anion-exchange resin IRA900 purifying, with the deionization washing resin of 3 times of resin volumes, use again the desorb of 0.1mol/L hydrochloric acid, collect concentration and be higher than the stripping liquid of 1mg/ml and be concentrated into 200ml.Transfer pH2.8 with halogen-containing nonpolar adsorption resin SP207 purifying this concentrated solution, with the deionization of 3 times of resin volumes washing resin, the ethanol desorb with 2%, collection R/O ratio is greater than 20 stripping liquid and be concentrated into 25ml, add again 12.5ml ethanol after at room temperature stirring a small amount of crystal seed of adding, continue to be stirred to mother liquid concentration and no longer obviously reduce, stop to stir rear filtration, dried crystals, get crystal 5 .0g, total recovery 41.3%, the weight purity 99.53% of solid, titration content 99.53%.
Embodiment 4
Get GSH extract 2L, contain GSH11.1g.Transfer to pH4.0 with gel-type weak basic anion exchange resin IRA67 purifying, with the deionization washing resin of 3 times of resin volumes, use again the desorb of 0.1mol/L hydrochloric acid, collect concentration and be higher than the stripping liquid of 1mg/ml and be concentrated into 200ml.Transfer pH2.8 with halogen-containing nonpolar adsorption resin SP207 purifying this concentrated solution, deionization washing resin with 3 times of resin volumes, ethanol desorb with 2% is collected R/O ratio greater than 20 stripping liquid and is concentrated into 20ml, at room temperature stirs to add 10ml ethanol after adding a small amount of crystal seed again, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 4 .1g, total recovery 36.9%, the weight purity 99.56% of solid, titration content 99.55%.
Embodiment 5
Get GSH extract 2L, contain GSH12.6g.Transfer to pH4.0 with macroporous type weak basic anion exchange resin D301 purifying, with the deionization washing resin of 3 times of resin volumes, use again the desorb of 0.1mol/L hydrochloric acid, collect concentration and be higher than the stripping liquid of 1mg/ml and be concentrated into 170ml.Transfer pH2.8 with halogen-containing nonpolar adsorption resin SP207 purifying this concentrated solution, deionization washing resin with 3 times of resin volumes, ethanol desorb with 2% is collected R/O ratio greater than 20 stripping liquid and is concentrated into 30ml, at room temperature stirs to add 15ml ethanol after adding a small amount of crystal seed again, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 4 .6g, total recovery 35.9%, the weight purity 98.55% of solid, titration content 99.84%.
Embodiment 6
Get GSH extract 2L, contain GSH12.6g.Transfer to pH3.0 with gel type strong base anionite-exchange resin FPA40 purifying, with the deionization washing resin of 3 times of resin volumes, use again the desorb of 0.1mol/L hydrochloric acid, collect concentration and be higher than the stripping liquid of 1mg/ml and be concentrated into 170ml.Transfer pH2.8 with halogen-containing nonpolar adsorption resin SP207 purifying this concentrated solution, deionization washing resin with 3 times of resin volumes, ethanol desorb with 2% is collected R/O ratio greater than 20 stripping liquid and is concentrated into 20ml, at room temperature stirs to add 10ml ethanol after adding a small amount of crystal seed again, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 4 .4g, total recovery 34.9%, the weight purity 98.91% of solid, titration content 99.81%.
Embodiment 7
Get GSH extract 2L, contain GSH12.9g.Transfer to pH3.0 with gel type strong base anionite-exchange resin FPA40 purifying, with the deionization washing resin of 3 times of resin volumes, use again 4% acetic acid desorb, collect concentration and be higher than the stripping liquid of 1mg/ml and be concentrated into 170ml.Transfer pH2.8 with halogen-containing nonpolar adsorption resin SP207 purifying this concentrated solution, deionization washing resin with 3 times of resin volumes, ethanol desorb with 2% is collected R/O ratio greater than 20 stripping liquid and is concentrated into 20ml, at room temperature stirs to add 10ml ethanol after adding a small amount of crystal seed again, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 4 .6g, total recovery 35.7%, the weight purity 98.59% of solid, titration content 99.85%.
Embodiment 8
Get GSH extract 2L, contain GSH12.1g.Transfer to pH3.0 with gel type strong base anionite-exchange resin FPA40 purifying, with the deionization washing resin of 3 times of resin volumes, use again the desorb of 0.05mol/L sulfuric acid, collect concentration and be higher than the stripping liquid of 1mg/ml and be concentrated into 170ml.Transfer pH2.8 with halogen-containing nonpolar adsorption resin SP207 purifying this concentrated solution, deionization washing resin with 3 times of resin volumes, ethanol desorb with 2% is collected R/O ratio greater than 20 stripping liquid and is concentrated into 20ml, at room temperature stirs to add 10ml ethanol after adding a small amount of crystal seed again, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 4 .8g, total recovery 39.7%, the weight purity 98.50% of solid, titration content 99.55%.
Embodiment 9
Get GSH extract 2L, contain GSH12.6g.Transfer to pH5.0 with gel type strong base anionite-exchange resin FPA40 purifying, with the deionization washing resin of 3 times of resin volumes, use again the desorb of 0.1mol/L hydrochloric acid, collect concentration and be higher than the stripping liquid of 1mg/ml and be concentrated into 100ml.Transfer pH2.8 with halogen-containing nonpolar adsorption resin SP207 purifying this concentrated solution, deionization washing resin with 3 times of resin volumes, ethanol desorb with 2% is collected R/O ratio greater than 20 stripping liquid and is concentrated into 16ml, at room temperature stirs to add 8ml ethanol after adding a small amount of crystal seed again, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 3 .8g, total recovery 30.2%, the weight purity 98.91% of solid, titration content 98.81%.。
Embodiment 10
Obtain the stripping liquid of anionite-exchange resin and concentrated according to the method for embodiment 1.Transfer pH4.0 with halogen-containing nonpolar adsorption resin SP207 purifying this concentrated solution, deionization washing resin with 3 times of resin volumes, ethanol desorb with 2% is collected R/O ratio greater than 20 stripping liquid and is concentrated into 8ml, at room temperature stirs to add 4ml ethanol after adding a small amount of crystal seed again, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 1.8g, yield is 18.7%, the weight purity 98.91% of solid, titration content 98.81%.。
Embodiment 11
Obtain the stripping liquid of anionite-exchange resin and concentrated according to the method for embodiment 1.This concentrated solution is transferred pH2.8 BAC spheric active carbon purifying, deionization washing gac with 3 times of column volumes, ethanol desorb with 50% is collected R/O ratio greater than 20 stripping liquid and is concentrated into 12ml, at room temperature stirs to add 6ml ethanol after adding a small amount of crystal seed again, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 4 .1g, yield is 48.4%, the weight purity 99.12% of solid, titration content 99.25%.
Embodiment 12
Obtain the stripping liquid of polymeric adsorbent according to the method for embodiment 1, be concentrated into 15ml, add again the 7.5ml Virahol after at room temperature stirring a small amount of crystal seed of adding, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 4 .9g, yield is 65.2%, the weight purity 99.73% of solid, titration content 99.78%.
Embodiment 13
Obtain the stripping liquid of polymeric adsorbent according to the method for embodiment 1, and be concentrated into 15ml, add again the 15ml50% Virahol after at room temperature stirring a small amount of crystal seed of adding, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 4 .2g, yield is 58.4%, the weight purity 98.81% of solid, titration content 99.34%.
Embodiment 14
Obtain the stripping liquid of polymeric adsorbent according to the method for embodiment 1, be concentrated into 15ml, add again 15ml acetone after at room temperature stirring a small amount of crystal seed of adding, continuing to be stirred to mother liquid concentration no longer obviously reduces, stop to stir rear filtration, dried crystals, get crystal 4 .5g, yield is 50.1%, the weight purity 98.07% of solid, titration content 98.55%.
Claims (15)
1. the method for separation and purification reduced glutathion from the fermentation extract that contains reduced glutathion said method comprising the steps of:
1) described fermentation extract is processed in turn through anionite-exchange resin, non-polar adsorbent, obtained containing the solution of reduced glutathion;
2) concentrated, crystallization, the dry above-mentioned solution that contains reduced glutathion.
2. method according to claim 1 is characterized in that, described anionite-exchange resin is strongly basic anion exchange resin, weak base anion-exchange resin, macroporous type anionite-exchange resin or gel-type anionite-exchange resin.
3. method according to claim 1 is characterized in that, the content of reduced glutathion is 5-10mg/ml in the described fermentation extract.
4. method according to claim 2 is characterized in that, described strongly basic anion exchange resin is FPA40; Weak base anion-exchange resin is D301; Macroporous type anionite-exchange resin is IRA900; Or gel-type anionite-exchange resin is IRA67.
5. method according to claim 1 is characterized in that, before anion exchange process, is 3.0-5.0 with the pH regulator of described fermentation extract, preferred 3.5-4.0.
6. method according to claim 5 is characterized in that, through behind the anionite-exchange resin, with 2-4 doubly, the deionization of preferred 3 times of resin volumes washing resin is used 0.05-0.2mol/L again, and the acidolysis of preferred 0.1mol/L is inhaled, and flow velocity is 1-2BV/h.
7. method according to claim 6 is characterized in that, described acid is selected from hydrochloric acid, sulfuric acid, formic acid or acetic acid.
8. method according to claim 1 is characterized in that, described non-polar adsorbent is selected from the polystyrene polymeric adsorbent, such as XAD1600; Halogen-containing polystyrene polymeric adsorbent is such as SP207; Or gac, such as the BAC spheric active carbon.
9. method according to claim 1 is characterized in that, before processing with non-polar adsorbent, is 1.0-4.0 with the pH regulator of described fermentation extract, preferred 2.5-3.0.
10. method according to claim 9 is characterized in that, through behind the nonpolar adsorption resin, with 2-4 doubly, the deionization of preferred 3 times of resin volumes washing resin is used 0.5-5%, preferred 2% aqueous solutions of organic solvent desorb, and flow velocity is less than 1BV/h.
11. method according to claim 9 is characterized in that, described organic solvent is selected from methyl alcohol, ethanol, Virahol or acetone.
12. method according to claim 1 is characterized in that step 2) described crystallisation step comprises the adding crystal seed, adds the solvent that dissolves each other with water or the solvent-water mixture that dissolves each other with water and the step that stirs again.
13. method according to claim 12 is characterized in that, the described solvent that dissolves each other with water is selected from lower alcohol or acetone.
14. method according to claim 13 is characterized in that, described lower alcohol is selected from methyl alcohol, ethanol or Virahol.
15. method according to claim 13 is characterized in that, the concentration of solvent-water mixture that described and water dissolves each other is 20-80%, preferred 50%.
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| CN105566445A (en) * | 2016-01-13 | 2016-05-11 | 浙江海正药业股份有限公司 | Method for separating and purifying reduced glutathione |
| CN105713069A (en) * | 2016-03-11 | 2016-06-29 | 浙江大学 | Bacilysin purification method |
| CN106526004A (en) * | 2016-10-14 | 2017-03-22 | 安琪酵母股份有限公司 | Method for detecting oxidized glutathione impurities in glutathione-rich yeast extract |
| CN107686503A (en) * | 2016-08-05 | 2018-02-13 | 北大方正集团有限公司 | A kind of method for purifying glutathione |
| CN108129550A (en) * | 2017-12-21 | 2018-06-08 | 广州白云山天心制药股份有限公司 | A kind of crystal form of reduced glutathione and preparation method thereof |
| CN114249794A (en) * | 2021-12-22 | 2022-03-29 | 深圳瑞德林生物技术有限公司 | Method for synthesizing oxidized glutathione |
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| CN105566445A (en) * | 2016-01-13 | 2016-05-11 | 浙江海正药业股份有限公司 | Method for separating and purifying reduced glutathione |
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| CN108129550A (en) * | 2017-12-21 | 2018-06-08 | 广州白云山天心制药股份有限公司 | A kind of crystal form of reduced glutathione and preparation method thereof |
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