CN103364564A - Intolerance test in animal - Google Patents
Intolerance test in animal Download PDFInfo
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- CN103364564A CN103364564A CN201210090821XA CN201210090821A CN103364564A CN 103364564 A CN103364564 A CN 103364564A CN 201210090821X A CN201210090821X A CN 201210090821XA CN 201210090821 A CN201210090821 A CN 201210090821A CN 103364564 A CN103364564 A CN 103364564A
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- intolerance
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Abstract
The invention discloses a method for diagnosing the sensibility or the intolerance of ingredients in companion animals. The method comprises a step of collecting a sample to detect existence of an antibody aiming at a concrete ingredient or composition. The sample can be saliva for detecting the existence of an IgA or IgM antibody aiming at the concrete ingredient or composition.
Description
Technical field
Present disclosure relates to composition (ingredient) the intolerance test in the companion animals.
Summary of the invention
According to present disclosure, diagnose testing system is provided, this system is used for examination or test in composition susceptibility or the intolerance of animal especially companion animals such as dog, cat, rabbit, hamster and Malaysia and China.
Can assess with saliva health or the morbid state of animal as diagnostic tool.
Realized the common anaphylactogen of problem and the composition intolerance of peptide of causing in dog or cat tested.This intolerance and test be used to inflammatory bowel disease (little ecologic disturbance in " intestines leakage " syndrome, the intestines) or the cereal relevant with other symptoms of unfavorable composition reaction-as but be not limited to wheat and other seitans, corn and soybean.
Used antigen is about (for) the be untreated peptide extract of height affinity purification of component substances.This will be to surpass 95% purity and about 99% purity preferably.Can will be categorized as such as the composition reactivity by anti-IgA in the saliva and/or anti-IgM horizontal survey:
Feminine gender is below the zero standard contrast;
Weak reactivity; A little higher than zero standard; Clinical meaning is unknown;
Middle (intermediate) level of reactivity; May have reactivity and composition is preferably avoided;
Medium (medium) level of reactivity; Should be avoided; And
Strong reactivity, composition must be avoided.
Embodiment
Need to be convenient to select be suitable for the pet composition of selected animal, thus the physiological make-up of the potpourri of prefabricated, balance and comprehensive component composition or large composition and small component and pet or companion animals, metabolism structure, biochemical construct or genetic structure between have compatibility.
The main example of composition susceptibility or intolerance is to wheat or other gluten components, for example intolerance of rye, rice, millet and oat.A kind of form of present disclosure relates to for the composition susceptibility of diagnosis companion animals or the method for intolerance, said method comprising the steps of: at first collect saliva sample; The examination saliva sample is to detect for the IgA of concrete composition or composition or at least a the existing in the IgM antibody.
The composition intolerance is wheat or other seitan susceptibility or intolerance, at least a in corn or soybean, beef or other meat or fish protein susceptibility or intolerance or dairy products, eggs, other cereals, botanical (botanicals), the oils from seed or fish, botanical, vegetables or fruit susceptibility or the intolerance.
When intestines internal layer (lining) permeability, the susceptibility that the composition that delays is relevant occurs in intestines and stomach.When the inflammation of intestines internal layer or damage, gastrointestinal function is destroyed.Intestines leak so that large composition antigen can be absorbed in the body.Then to attack this antigen or these antigen and result be the antibody that produces for once being harmless, nontoxic composition to the system of defense of health.These IgA or IgG antibody and immune complex form in blood flow and are recycled to whole health, and they can destroy its hetero-organization along the road in health.In saliva or other body fluid, typically these reactants are IgA or IgM.
Term " pet " refers to domestic dog, cat or horse.Term " pet component composition " refers to be intended to allow component composition or the feed ingredient of pet picked-up.Diagnose testing system is provided, and this diagnose testing system is used for examination or test animal to composition susceptibility or the intolerance of the composition that causes problem that is present in the following: the pet food of pre-service or premix, meat, biscuit, dessert, snacks (treats), spill material (sprinkles), candy and other forms of pet food, flavouring, medicine, replenishers and be used for injecting, the treatment product of oral or local application.
Although present disclosure in this respect in concrete parameter and equipment have been discussed, be appreciated that parameter can be different and can implement disclosed method and not deviate from the scope of present disclosure with different equipment.
Be used for determining existing the method for composition allergy or composition intolerance to comprise that (a) determines at least a in the saliva of the food that is present in composition (dietary) antigen or peptide or other mucous membrane liquid or other body fluid IgA or IgM antibody or the Multiple Antibodies and preferably surpass a kind of level in companion animals such as dog or cat or Malaysia and China, described composition is in saliva or other mucous membrane liquid or other humoral samples from animal; And the normal level of the level that (b) will determine in step (a) and described one or more antibody in mucous membrane liquid or other humoral samples compares.The possible outcome of this comparison comprises: (i) be lower than the normal level of dietary antigen antibody or show optimum condition near the normal level of dietary antigen antibody; And the normal level that (ii) is higher than dietary antigen antibody shows composition allergy or composition intolerance.
Can will be categorized as such as the composition reactivity by anti-IgA in the saliva and/or anti-IgM horizontal survey: feminine gender, below the zero standard contrast; Weak reactivity; A little higher than zero standard; Clinical meaning is unknown; The intermediate reactive level; May have reactivity and composition is preferably avoided; Medium level of reactivity; Should be avoided; And strong reactivity, composition must be avoided.
Can use the outer other forms of biofluid of desalivation for example urine, tears or milk or other mucous membrane secretion.
Can carry out the detection of antagonist with measuring.Mensuration includes but not limited to that ELISA test, RIA test, latex agglutination test, glass ball are measured (beads assay) and the protein group is measured.Preferred mensuration is the ELISA test.Can use other mensuration and can determine selection to measuring by those skilled in the art.
Normal reading derives from base measurement, and described base measurement is taken from the antibody of the individuality that does not have the symptom that relates to composition allergy or composition intolerance is measured.Obtain base measurement about test by observing antibody measurement to the individuality that do not have the symptom that relates to composition allergy or composition intolerance.For example, most of reading of measuring from the antibody of the individuality that does not have the symptom that relates to composition allergy or composition intolerance is lower than specific reading.Employing is for detection of the optical means of analyte and receptors bind, perhaps alternatively Electrochemical Detection can be arranged, wherein the combination of analyte directly or indirectly causes the activity change of the electroactive material (electroactive species) of adjacent electrode, and this also is used to measure.
For contrast dog or the cat of health, saliva IgA antibody horizontal is usually less than about 10U/ml (dog) or is lower than about 25U/ml (cat).For dog or the cat of health, saliva IgM antibody horizontal is usually less than about 25U/ml.
For the sick dog or the sick cat that suffer from moderate composition allergy and composition intolerance, saliva IgA antibody horizontal is usually at about 15U/ml (dog) or at about 30U/ml (cat).For sick dog or sick cat, saliva IgM antibody horizontal is at about 35U/ml.
For the sick dog or the sick cat that suffer from severe composition allergy and composition intolerance, saliva IgA antibody horizontal is usually less than about 20U/ml (dog) or about 35U/ml (cat).Saliva IgM antibody horizontal is usually at about 40U/ml (dog or cat).
The major part of saliva IgA antibody horizontal (about 98%) reading is lower than about 10U/ml (dog) or 25U/ml (cat) in normal healthy controls dog or cat.Equally, the major part of saliva IgM antibody horizontal (about 85%) reading is lower than about 25U/ml in normal healthy controls dog or cat.Yet, in the group of normal healthy controls dog or cat, there is some reading, it is higher than about 10U/ml (dog) or 25U/ml (cat).Extra high reading in normal healthy controls dog or cat can be indicated the intolerance to the dietary antigen of correspondence.
Claims (8)
1. one kind is used for diagnosis in the composition susceptibility of companion animals or the method for intolerance, and said method comprising the steps of: collect the sample of non-serum biofluid, described non-serum biofluid optionally is saliva or other non-serum body fluid; The described sample of examination is to detect for the IgA of concrete composition or composition or at least a the existing in the IgM antibody, and based on existence diagnosis composition susceptibility or the intolerance of described antibody, the antigen that wherein is used for test is the peptide extract about the height affinity purification of the component substances that is untreated.
2. method claimed in claim 1, the purity of wherein said highly purified extract is about 95% and preferably about 99% at least.
3. method claimed in claim 1 wherein is classified as such as the reactivity by composition as described in anti-IgA in the saliva and/or the anti-IgM horizontal survey: feminine gender, below the zero standard contrast; Weak reactivity; A little higher than described zero standard; Clinical meaning is unknown; The intermediate reactive level; May have reactivity and composition is preferably avoided; Medium level of reactivity; Should be avoided; And strong reactivity, composition must be avoided.
4. each described method in the claims 1 to 3, further wherein said sample is saliva or other body fluid, and described method comprises from described saliva or other humoral samples collects part of detecting, and wherein said part of detecting is the sample in the described examination step.
5. each described method in the claim 1 to 4, described method comprise to be utilized enzyme connection determining adsorption (ELISA) test macro to detect for concrete composition or becomes the existence of the antibody of grouping.
6. each described method in the claim 1 to 5, wherein about 15U/ml (dog) or 30 (slight at least insensitivity or the intolerance of at least one indication in the IgA level of U/ml (cat); Perhaps the IgM level of the 35U/ml of dog or cat is indicated slight at least insensitivity or intolerance.
7. each described method in the claim 1 to 6, wherein said composition intolerance are at least a in the following: wheat or other seitans, corn or soybean, beef or other meat or fish protein or dairy products, eggs, other cereals, botanical, oils, botanical, vegetables or fruit from seed or fish.
8. each described method in the claim 1 to 7, wherein said composition are in the following any one: the pet food of premix, meat, biscuit, dessert, snacks, spill material, candy and other forms of pet food, flavouring, medicine, replenishers and be used for the treatment product of injection, oral or local application.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210090821XA CN103364564A (en) | 2012-03-30 | 2012-03-30 | Intolerance test in animal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210090821XA CN103364564A (en) | 2012-03-30 | 2012-03-30 | Intolerance test in animal |
Publications (1)
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|---|---|
| CN103364564A true CN103364564A (en) | 2013-10-23 |
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| CN201210090821XA Pending CN103364564A (en) | 2012-03-30 | 2012-03-30 | Intolerance test in animal |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030143627A1 (en) * | 2001-08-14 | 2003-07-31 | Aristo Vojdani | Saliva test for detection of food allergy and intolerance |
| CN1547025A (en) * | 2003-12-03 | 2004-11-17 | 刘春杰 | Highly sensitive enzyme immunoassay detection kit |
| US20100190190A1 (en) * | 2009-01-26 | 2010-07-29 | Dodds W Jean | Food sensitivity testing in animals |
| US20100190191A1 (en) * | 2009-01-26 | 2010-07-29 | Dodds W Jean | Multi-stage nutrigenomic diagnostic food sensitivity testing in animals |
| CN102341705A (en) * | 2009-03-05 | 2012-02-01 | 味之素株式会社 | Diagnostic reagent for crohn's disease |
-
2012
- 2012-03-30 CN CN201210090821XA patent/CN103364564A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030143627A1 (en) * | 2001-08-14 | 2003-07-31 | Aristo Vojdani | Saliva test for detection of food allergy and intolerance |
| CN1547025A (en) * | 2003-12-03 | 2004-11-17 | 刘春杰 | Highly sensitive enzyme immunoassay detection kit |
| US20100190190A1 (en) * | 2009-01-26 | 2010-07-29 | Dodds W Jean | Food sensitivity testing in animals |
| US20100190191A1 (en) * | 2009-01-26 | 2010-07-29 | Dodds W Jean | Multi-stage nutrigenomic diagnostic food sensitivity testing in animals |
| CN102341705A (en) * | 2009-03-05 | 2012-02-01 | 味之素株式会社 | Diagnostic reagent for crohn's disease |
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Application publication date: 20131023 |