CN103361167A - Biological preparation prepared from high-omega-3 fatty acid and oxidation-resisting nutritional oil of high-omega-3 fatty acid and microbial conversion technology - Google Patents
Biological preparation prepared from high-omega-3 fatty acid and oxidation-resisting nutritional oil of high-omega-3 fatty acid and microbial conversion technology Download PDFInfo
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- CN103361167A CN103361167A CN2012100895366A CN201210089536A CN103361167A CN 103361167 A CN103361167 A CN 103361167A CN 2012100895366 A CN2012100895366 A CN 2012100895366A CN 201210089536 A CN201210089536 A CN 201210089536A CN 103361167 A CN103361167 A CN 103361167A
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Abstract
The invention discloses a biological preparation prepared from a high-omega-3 fatty acid and an oxidation-resisting nutritional oil of the high-omega-3 fatty acid and a microbial conversion technology. The biological preparation is prepared from natural oil crop seeds which are rich in the omega-3 fatty acid, distillers' grains and a microbial enzyme. The natural oil crop seeds are preliminarily fermented and converted so that the ratio of the omega-3 fatty acid in an oil can be increased, in addition, the phenols and the polyenes in the distillers' grains are subjected to unsaturated free radical addition and connected into an oxidation-resisting group so that the shelf life of the oil can be prolonged and the color, the smell and the taste of the oil can be improved, and moreover, the materials are squeezed physically at a lower temperature to obtain the product. Compared with the traditional preparation method, the microbial conversion technology is stronger in bionergy, is reasonable in process and low in processing cost and can not lead to the generation of harmful byproducts. In addition, all the raw materials can be grown widely, are easy to culture, are feeding or healthcare natural plants and byproducts from grain oil production, are non-toxic and edible, can be fermented at a low temperature with the least energy being consumed and can be processed closely without discharge of three wastes. The dry meal generated by adopting the microbial conversion technology can be fed to livestock as a feeding biological supplement to produce high-omega-3 fatty acid eggs and milks, and the main product can be used for developing a series of nutritional oil and healthcare oil of the high-omega-3 fatty acid.
Description
(1) technical field: the present invention relates to a kind of microbial transformation production method of nutritive food, particularly a kind of biotechnological formulation and microbiological transformation technology that can improve omega-fatty acid in the nutritional oil and antioxidant property thereof.
(2) background technology: people are common to notice that should not too much eat saturated fatty acid is main livestock raised for meat fat, mainly eats the understanding mistaken ideas that vegetables oil can be healthy and safe but still exist.The World Health Organization and Food and Argriculture OrganizationFAO hold " ω-3 series fatty acid and healthy international conference " (1990.3.23, Washington) statement: " in view of existing positive evidence shows; ω-3 series fatty acid has indispensable and irreplaceable effect to human development and health; suggestion all should contain ω-3 series fatty acid in baby and adult's food ".
In recent decades, the way that solves this indispensable lipid acid is: use organic alcoholysis technology, from the linseed oil of Oils,glyceridic,cod-liver, marine alga and the inedibilities such as cod (Alcoholysis), extract the mixture with triglycerides thing, adopt again the organic chemical synthesis method to change into EPA and DHA, this kind technical matters all is difficult to satisfy the great demand of edible and health industry from contrasts such as resource, technical difficulty and renewable cost of development.
Recently, China has carried out and has used physical squeezing, solvent method (sherwood oil method), SC-CO
2Supercritical extraction and water vapor (250 ℃) carry out the methods such as cracking to linolein, carry out the exploitation of omega-fatty acid series product, above development technique exists in various degree: extraction yield is not high, facility investment is large, temperature of reaction is high, produce easily side reaction, unsaturated free group is oxidized to the shortcomings such as trans fatty acid nuisance, to objectionable constituent such as linamarin in the oleum lini and white arteries and veins root glycosides, it is large to separate the elimination difficulty, there are the shortcomings such as the easy oxidation of long-chain unsaturated fatty acid too in radix cynanchi atrati-perilla oil, suddenly treats further to adopt economical, conversion technology applicable and safety is developed.
(3) summary of the invention
1, radix cynanchi atrati-purple perilla oilseeds, the biotechnological formulation that Maotai-flavor liquor grain and proteolytic ferment are developed into, detect through for many years, preparation adopts that unit plantation output is high, the equal good plant (Latin literary fame: perilla frutescent arguta, perilla furtesceng prispa, perilla fratescens (L) Britt) of oil length and a-linolenic acid (ALA); Still residual in the Guizhou Maotai-flavor liquor production waste matter have couple Gao Re and stable organic oxidation-resistant things such as phenols of alkali; The proteolysis microbial enzyme adopts: Aspergillug oryzae, Aspergillus niger As3.350, Saccharomyces ceremisiae 1300.
2, lipid acid is carried out the preposition fermentation of microbial enzyme take the a-linolenic acid as main oilseeds and transform, the unsaturated free radical of long-chain polyenoid of ALA is combined with anti-oxidant phenols, produce resistance of oxidation; Simultaneously, through the conversion of proteolysis microbial enzyme to unsaturated group, under the participation of remaining ethanol, fractional saturation lipid acid is easier to be stayed in the seed dregs of rice in waste matter, and the polyenoid long-chain unsaturated fatty acid relatively ratio in oil improves; Under the low temperature fermentation condition, can avoid changing into the generation of the harmful side products such as trans fatty acid or erucic acid, can improve and promote mouthfeel and the quality guaranteed period of edible nourishing oil.After the preposition low temperature fermentation, material is dewatered, carry out physical squeezing again, can obtain all good nutrient health-care function oil of color, seed dregs of rice by product is the feeding biotechnological formulation that egg fowl and milk animal output are rich in the omega-3 unsaturated fatty acid livestock product.
3, the beneficial effect of the invention: compare with conventional food vegetables oil processing technology, under the low temperature fermentation condition, the phenols that Nai Gaore in the Maotai-flavor liquor grain and alkaline environment are stable, with the unsaturated combined with radical of the serial polyenoid of ω-3, produce stable resistance of oxidation, this contains the highest poor shortcoming of crude vegetal resistance of oxidation of ALA to have solved radix cynanchi atrati-purple perilla, impel its extensive popularization in the nutritive health-care field to lay the first stone: after fermentation transforms, partly saturated fatty acid is relatively stable remains in the withered dregs of rice, and omega-fatty acid ratio in oil increases 3%-5%; Through the preposition conversion of microbial protease, the polyenoid long-chain unsaturated fatty acid is in receptor (comprising human body) Digestive tract, and is also easier in conversions such as DHA, promoted the specific absorption of this nutritive ingredient and the increase of nourishing function.The facility investment of this kind microbial transformation fermentation method for producing is few, productive rate is high, and tooling cost is low, achieves many things at one stroke, and economic benefit significantly improves.
(4) embodiment
Get two each 30-50 kilogram of strain purple perilla (radix cynanchi atrati) seed (dry weight, lower same), Maotai-flavor liquor groove 5-30 kilogram, the hydrolysising microorganism enzyme is an amount of → and stirrer homogenization → rod formula pulverizer coarse crushing is to 30-50 ℃ of 0.5mm → enter hydrothermal pot (200 liter) → insulation, through solid state fermentation → discharging in 12-72 hour → cold blast engine dehydration → ultraviolet lamp sterilization → high pressure pressure filter fluid/withered dregs of rice separation.
The gained liquid oil is aobvious yellowish transparent, the special similar amber aroma of tool, and color further promotes, and after the phenols grafting addition reaction of the unsaturated free radical of ω-3 polyenoid and heat-resisting alkali resistant, makes long-chain unsaturated fatty acid obtain resistance of oxidation, extends the shelf life; Oil yield can reach 40%-50%; The a-linolenic acid content can reach 62%-66%, improves 3%-5% than conventional physical milling process or solvent method (sherwood oil method); After microbial fermentation transforms, be more conducive to the absorption of human body, to the more unsaturated DHA lipid acid conversion of long-chain, strengthen nutrient health-care function, more be conducive to the gene disease of the multiple harm humans of assisting therapy.This product may be used on edible nourishing mixed oil, health care (oil) product, high density assisting therapy omega-fatty acid oil, cosmetic products and characteristic tourism food etc.
The withered dregs of rice of by product that this production method produces are the feeding biological supplementing preparation of best egg fowl, milk animal and livestock raised for meat production high omega-3 fatty acid nutrient egg, breast and meat.
Important vegetables oil main fatty acid and healthy contrast table thereof
Claims (2)
1. the biotechnological formulation produced of high omega-3 fatty acid and antioxidant property nutritional oil thereof
It is characterized in that: the present invention is different from tradition and makes oilseeds popular and directly squeeze production method, select first purple perilla (radix cynanchi atrati) oilseeds, Maotai-flavor liquor grain and proteolytic ferment to be developed into unique oil sources and the integrated biotechnological formulation of function component raw material, this preparation can be produced the nutritional oil that improves omega-fatty acid ratio and resistance of oxidation thereof simultaneously, and its withered dregs of rice by product also is the biotechnological formulation that egg fowl and milk animal produce high omega-3 fatty acid egg breast simultaneously.
2. high omega-3 fatty acid and antioxidant property nutritional oil thereof the microbiological transformation technology of producing
It is characterized in that: the present invention is different from solvent method or supercritical extraction technique, adopt under low temperature, the neutrallty condition, under the effect of microbial proteinous lytic enzyme, phenols becomes antioxidant groups with the omega-fatty acid combined with radical in the vinasse, simultaneously, promote saturated fatty acid to stay the withered dregs of rice, the omega-fatty acid ratio promotes, carry out again the cryogenic high pressure physical squeezing, the biological technology products of achieving many things at one stroke.
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| CN201210089536.6A CN103361167B (en) | 2012-03-30 | 2012-03-30 | Biological preparation prepared from high-omega-3 fatty acid and oxidation-resisting nutritional oil of high-omega-3 fatty acid and microbial conversion technology |
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| CN201210089536.6A CN103361167B (en) | 2012-03-30 | 2012-03-30 | Biological preparation prepared from high-omega-3 fatty acid and oxidation-resisting nutritional oil of high-omega-3 fatty acid and microbial conversion technology |
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| CN103361167A true CN103361167A (en) | 2013-10-23 |
| CN103361167B CN103361167B (en) | 2015-03-11 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108142672A (en) * | 2016-12-02 | 2018-06-12 | 农业公司法人格林格莱斯株式会社 | Fodder compound using perilla cake and preparation method thereof |
| CN109880693A (en) * | 2019-01-18 | 2019-06-14 | 范志祥 | A kind of anti-oxidant fermentation process of high omega-3 fatty acid content nutritional oil |
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| CN1114141A (en) * | 1995-04-06 | 1996-01-03 | 华南理工大学 | Nutritive mixed oil containing DHA and EPA and preparing process thereof |
| CN1130485A (en) * | 1995-11-14 | 1996-09-11 | 杜鸿钧 | Omega-3omega-6 fatty acid series health food |
| CN1377591A (en) * | 2001-03-30 | 2002-11-06 | 曾明果 | High omega-3 fatty acid and low cholesterol fodder additive for laying fowls |
| CN101099562A (en) * | 2006-07-07 | 2008-01-09 | 赵恩厚 | Omega-3 unsaturated fatty acids appetizer oil and producing method and application thereof |
| CN101489403A (en) * | 2006-12-29 | 2009-07-22 | 又石大学企业与教育合作中心 | Method for preparing processed edible oil having a highly enriched buttery flavor and a very low content of trans fatty acids and processed edible oil prepared by the same |
| CN101703170A (en) * | 2009-11-12 | 2010-05-12 | 四川铁骑力士实业有限公司 | Poultry feed and preparation method thereof |
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2012
- 2012-03-30 CN CN201210089536.6A patent/CN103361167B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1114141A (en) * | 1995-04-06 | 1996-01-03 | 华南理工大学 | Nutritive mixed oil containing DHA and EPA and preparing process thereof |
| CN1130485A (en) * | 1995-11-14 | 1996-09-11 | 杜鸿钧 | Omega-3omega-6 fatty acid series health food |
| CN1377591A (en) * | 2001-03-30 | 2002-11-06 | 曾明果 | High omega-3 fatty acid and low cholesterol fodder additive for laying fowls |
| CN101099562A (en) * | 2006-07-07 | 2008-01-09 | 赵恩厚 | Omega-3 unsaturated fatty acids appetizer oil and producing method and application thereof |
| CN101489403A (en) * | 2006-12-29 | 2009-07-22 | 又石大学企业与教育合作中心 | Method for preparing processed edible oil having a highly enriched buttery flavor and a very low content of trans fatty acids and processed edible oil prepared by the same |
| CN101703170A (en) * | 2009-11-12 | 2010-05-12 | 四川铁骑力士实业有限公司 | Poultry feed and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108142672A (en) * | 2016-12-02 | 2018-06-12 | 农业公司法人格林格莱斯株式会社 | Fodder compound using perilla cake and preparation method thereof |
| CN109880693A (en) * | 2019-01-18 | 2019-06-14 | 范志祥 | A kind of anti-oxidant fermentation process of high omega-3 fatty acid content nutritional oil |
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| CN103361167B (en) | 2015-03-11 |
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