CN103357067A - Fibroin-base artificial ligament repair material and preparation method thereof - Google Patents
Fibroin-base artificial ligament repair material and preparation method thereof Download PDFInfo
- Publication number
- CN103357067A CN103357067A CN2013102842122A CN201310284212A CN103357067A CN 103357067 A CN103357067 A CN 103357067A CN 2013102842122 A CN2013102842122 A CN 2013102842122A CN 201310284212 A CN201310284212 A CN 201310284212A CN 103357067 A CN103357067 A CN 103357067A
- Authority
- CN
- China
- Prior art keywords
- artificial ligament
- ligament repair
- repair materials
- fibroin
- silk fibroin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention discloses a fibroin-base artificial ligament repair material and a preparation method thereof. The ligament repair material comprises stents and laid-in yarns, wherein the stents are produced by carrying out biaxial laid-in warp knitting on natural mulberry silks; the laid-in yarns are regenerated silkworm silk fibroin filament yarns and regenerated silkworm silk nanofiber yarns. The preparation method specifically comprises the following steps of: (1) preparing biaxial laid-in warp knitting stent materials; (2) preparing regenerated silk fibroin filament yarns by utilizing a dry or wet spinning method; (3) preparing and dissloving a regenerated silk fibroin film, and preparing nanometer silk fibroin fiber yarns by utilizing an electrostatic spinning method; (4) taking a pure fibroin warp knitting material as a base material, taking the regenerated silkworm silk fibroin filament yarns and the nanometer silk fibroin fiber yarns as the laid-in yarns, weaving the base material and the laid-in yarns into the warp knitting stent material to obtain the artificial ligament repair material, and sewing two sides of a ligament material into a columnar structure. The preparation method is simple and efficient, and the artificial ligament repair material prepared by utilizing the preparation method has excellent compatibility, degradability and mechanical properties.
Description
Technical field
The present invention relates to a kind of artificial ligament repair materials, be specifically related to a kind of fibroin base artificial ligament repair materials and preparation method thereof, belong to the Implantable Medical Device technical field.
Background technology
Along with the rise of tissue engineering, artificial ligament obtains people's attention again as the Healing in Anterior Cruciate Ligament Reconstruction material, for ligament is provided by the effective approach that provides.The development of the subjects such as the comprehensive life sciences of tissue engineering artificial ligament and engineering uses biological fiber and synthetic material as tough belt supporting frame, promotes in vitro and in vivo sticking and grow of cell and tissue, with alternative normal anterior cruciate ligament.
In the prior art, the artificial ligament material that belongs to study hotspot and obtained certain progress is the permanent type ligament material, as: the carbon fibre ligament; Dacron ligament, material are polyethylene terephthalate; Gore-Tex ligament, material are politef; LAD strengthens ligament, and material is polypropylene; LARS polyester ligament etc.But above-mentioned ligament material is Shortcomings all: the carbon fibre degeneration is fast, and enough tensile strength can not be provided, and histocompatibility is poor, and the cracking granule causes heavier inflammatory reaction, and compliance is poor; The Dacron ligament material is prone to complication, such as ligament rapture and synovitis; Although Gore-Tex ligament material intensity is large, rebuild early stage results fair, can continue in time the generation degeneration, develop complications; LAD strengthen ligament material implant can cause that reactivity is oozed out behind the knee joint inside, joint fibrosis and synovitis, increase infection chance; LARS polyester ligament can't degradation in vivo, can not guide Growth of Cells to promote the formation of human body self ligament tissue.So suitable artificial ligament repair materials at first will possess excellent biocompatibility and degradability.
Fibroin albumen is the natural polymer fibrin that extracts from silkworm silk, has good biocompatibility and good physical and chemical performance, becomes in recent years one of focus object of tissue engineering bracket material research.Studies show that silk fibroin material has than other natural fibers and the unique mechanical property of high-performance synthetic fibers; Fiber surface is easy to chemical covalent modification and sticks site and cytokine, can process by distinct methods to obtain variform and change surface property; Can slow degradation be aminoacid in vivo, and catabolite to organism without danger.
The people such as Altman adopt fibroin design organization engineering anterior cruciate ligament, its 3-D space structure is referred to as the steel cable type structure, its mechanical property and fatigue durability are all satisfactory (referring to Gregory H. Altman, David L. Kaplan et al. Biomaterials. 23 (2002) 4131 – 4141), but in the cell cultivation process, because the steel cable type ligament structure is tight, cell can only be attached to the surface of support, can't enter internal stent; The people such as Haifeng Liu have studied fibroin frame-type ligament material, the silk stent material is immersed the final 3-D of formation space structure behind the silk fibroin protein solution (referring to Haifeng Liu, but the porous fibroin material that forms stable restive Hongbin Fan et al. Biomaterials. 29 (2008) 662 – 674); The people such as Laurencin are take PLLA as raw material, weave banded timbering material and form the 3-D space structure (referring to Cooper JA, Lu HH et al. Biomaterials. 26 (2005) 1523 – 1532), its ligament is that the fibre bundle interlacing becomes band, the intersecting angle at ligament stage casing and two ends is difference to some extent, but stage casing yarn line structure has intersection, can the some effects cell adhesion.
So, need the new artificial ligament repair materials of exploitation.
Summary of the invention
The object of the invention provides a kind of fibroin base artificial ligament repair materials, utilize natural mulberry silk, regeneration mulberry silk and regeneration mulberry silk nano-fibre yams jointly to make up, to obtain the material of good biocompatibility, improve cell adhering to and grow in the artificial ligament repair materials.
Another object of the present invention provides the preparation method of above-mentioned material.
For achieving the above object, the technical solution used in the present invention is, a kind of fibroin base artificial ligament repair materials comprises support and stuffer weft, and described support adopts twin shaft to consist of through volume to inlaid thread by natural mulberry silk; Stuffer weft is Regenerated Bombyx mori Silk Fibroin long filament and regeneration mulberry silk nano-fibre yams, and described stuffer weft inweaves in the warp knitting fabrics structure of support.
In the technique scheme, the natural mulberry silk consumption accounts for 30～70% of artificial ligament repair materials total amount; Regenerated Bombyx mori Silk Fibroin long filament consumption accounts for 10～30% of artificial ligament repair materials total amount; Regeneration mulberry silk nano-fibre yams consumption accounts for 20～40% of artificial ligament repair materials total amount.
In the technique scheme, described artificial ligament repair materials length is 27.5～41cm; The diameter of described Regenerated Bombyx mori Silk Fibroin long filament is 15～80 μ m; The diameter of described regeneration mulberry silk nano-fibre yams is 50～2000nm.
The diameter of artificial ligament repair materials disclosed by the invention can be according to the design of the diameter of the ligament that will repair, is preferably 0.8～1.5 times of the ligament diameter that will repair.
In the preferred technical scheme, described artificial ligament repair materials also comprises reinforcing fibre, and it inweaves in the warp knitting fabrics structure as stuffer weft, and consumption accounts for 10～90% of the total consumption of stuffer weft; Described reinforcing fibre is carbon fiber, superhigh molecular weight polyethylene fibers or polyester fiber.
The invention also discloses a kind of method for preparing above-mentioned artificial ligament repair materials, may further comprise the steps:
(1) adopt natural mulberry silk braiding twin shaft to inlaid thread through compiling timbering material, after obtain Bombyx mori Silk Fibroin through compiling timbering material through coming unstuck;
(2) natural mulberry silk prepares regenerated silk fibroin solution after coming unstuck, dissolve, dialysing, and adopts dry method or wet spinning to prepare the Regenerated Bombyx mori Silk Fibroin long filament;
(3) natural mulberry silk uses hexafluoroisopropanol or formic acid dissolving fibroin protein film through coming unstuck, preparing regenerated silk fibroin film after the dissolving, dialysis, drying, then adopts method of electrostatic spinning preparation regeneration mulberry silk nano-fibre yams;
(4) take fibroin through compiling timbering material as base material, Regenerated Bombyx mori Silk Fibroin long filament and regeneration mulberry silk nano-fibre yams inweave in the warp knitting fabrics structure as stuffer weft, reeling forms the artificial ligament material, the ligament material both sides is sewed up namely got described fibroin base artificial ligament repair materials at last.
In the technique scheme, in mass ratio, natural mulberry silk: Regenerated Bombyx mori Silk Fibroin long filament: regeneration mulberry silk nano-fibre yams is (30～70): (10～30): (20～40).
In the preferred technical scheme, in the step (4), the natural mulberry silk that prepared regenerated silk fibroin long filament in carbon fiber, superhigh molecular weight polyethylene fibers or polyester fiber and step (2), (3) and regeneration mulberry silk nano-fibre yams are inweaved step (1) preparation as stuffer weft makes up the artificial ligament repair materials jointly in compiling timbering material; The consumption of carbon fiber, superhigh molecular weight polyethylene fibers or polyester fiber accounts for 10～90% of the total consumption of stuffer weft.
Among the present invention, braiding all belongs to prior art through compiling timbering material, natural silk degumming, dissolving, dialysis, dry spinning, wet spinning, electrostatic spinning, coiling and sewing up, and those skilled in the art can select voluntarily according to product.The preferred condition of the present invention is as follows:
Its tissue employing of braiding through compiling timbering material in the above-mentioned steps (1) RSM twin shaft is weaved to tricot weft insertion machine, organizes technique, threading yarn technique as follows:
Organize technique:
GB1：2-3/3-2/1-0/2-3/1-0/0-1/2-3/1-0//
GB2：1-0/0-1//×4；
Threading yarn technique:
GB1: completely wear
GB2: completely wear;
Plane No.: E12
In the above-mentioned steps (2), the preparation of Regenerated Bombyx mori Silk Fibroin long filament is to be 4% silk fibroin water solution with natural mulberry silk through coming unstuck, form after the dissolving, dialysis treatment mass fraction, and it is positioned over stirring at low speed in Polyethylene Glycol (PEG) solution, and to be concentrated into mass fraction be 22%, 27%, 32% silk fibroin water solution; Be about 25 ℃ in temperature, within the resting period of 0-5d, carry out wet spinning, the spun filament cellulose fiber is immersed in the dehydrated alcohol, sealing places 24 h under the room temperature, carries out drawing-off in the deionized water more than 60 ℃, with one times of as-spun fibre drawing-off, in 120 ℃ of lower heat setting 30 min; Again the spun filament cellulose fiber is positioned over mass ratio and is among 2: 1 the 1-(3-dimethylaminopropyl)-3-ethyl carbodiimides (EDC)/N-maloyl imines (NHS), add by a certain percentage ethanol/water (V
C2H5OH/ V
H2O=90/10) mass fraction that is mixed with in is 4.5% crosslinked fluid, crosses 24 h, and drawing-off in the deionized water more than 60 ℃ is placed on 120 ℃ of lower heat setting 30 min, obtains the Regenerated Bombyx mori Silk Fibroin long filament of EDC/NHS after crosslinked.
In the above-mentioned steps (3), method of electrostatic spinning is to be 4～8% silk fibroin water solution with natural mulberry silk through coming unstuck, form after the dissolving, dialysis treatment mass fraction, and aqueous solution is poured on the vinyon flat board, and drying obtains fibroin membrane at ambient temperature; Regenerated Silk Fibroin Film is dissolved in hexafluoroisopropanol (HFIP) or the formic acid, to make mass fraction one week be 10～12% spinning liquid in vibration in 25 ℃ of water-baths of constant temperature, spinning liquid poured into carry out electrostatic spinning in the syringe, No. 9 syringe needles, spinning flow 2mL/h, adjust voltage and solidify distance, make field intensity reach 1kV/cm, make regeneration mulberry silk nano-fibre yams.
In the above-mentioned steps (4), adopt serger that the ligament material both sides are sewed up, the artificial ligament repair materials that obtains is column construction, overall length 27.5～41cm, parallel fiber zone length 1.5～5cm, two ends woollen yarn knitting zone length respectively is 10～15cm, and the consolidation zone length of field is 3cm, and diameter is 8～15mm.This ligament repair material has guaranteed the mechanical property of ligament structure take the warp knitting fabrics structure of natural mulberry silk weaving as base rack; Increase simultaneously regenerated silk fibroin long filament and regeneration mulberry silk nano-fibre yams in the inside of support, its fiber size is more conducive to guide Growth of Cells, thereby accelerate the formation of self ligament tissue, can in the process that self ligament tissue forms, degrade gradually simultaneously, reach the advantageous property of organizational project ligament, also for cambium provides support, and keep certain hour to cambium to have the biomechanics characteristic of growing.
Because technique scheme is used, the present invention compared with prior art has following advantages:
(1) the present invention is raw materials used is the pure natural fibroin albumen, has excellent biocompatibility, nontoxic, be beneficial to cell adhesion, growth and differentiation, and final catabolite is aminoacid, and is harmless; Has high mechanical strength when the artificial ligament repair materials of preparation has good biocompatibility and degradability thus;
(2) ligament repair material disclosed in this invention presents 3-D solid structure, has the feature of high porosity, high-specific surface area, can provide sufficient space to be beneficial to cell adhesion growth and nutrient substance enters, the discharge of metabolite;
(3) preparation method disclosed in this invention is simple, and easy operating is fit to suitability for industrialized production.
Description of drawings
Fig. 1 is through compiling the structure chart of support among the embodiment one;
Fig. 2 is the structural representation of artificial ligament repair materials among the embodiment one;
Fig. 3 is the longitudinal scanning Electronic Speculum figure of Regenerated Bombyx mori Silk Fibroin filament fiber among the embodiment one;
Fig. 4 is the scanning electron microscope (SEM) photograph of regeneration mulberry silk nano-fibre yams among the embodiment one.
The specific embodiment
The invention will be further described below in conjunction with drawings and Examples:
Embodiment one:
(1) its tissue employing of the braiding of natural mulberry silk through compiling timbering material RSM twin shaft is weaved to tricot weft insertion machine, organizes technique, threading yarn technique as follows:
A. organize technique:
GB1：2-3/3-2/1-0/2-3/1-0/0-1/2-3/1-0//
GB2：1-0/0-1//×4
B. threading yarn technique:
GB1: completely wear;
GB2: completely wear
C. plane No.: E12;
(2) be 5% silk fibroin water solution with natural mulberry silk through coming unstuck, form after the dissolving, dialysis treatment mass fraction, it is positioned over stirring at low speed in the PEG solution, and to be concentrated into mass fraction be 22% silk fibroin water solution; Be about 25 ℃ in temperature, within the resting period of 5d, carry out wet spinning, the spun filament cellulose fiber is immersed in the dehydrated alcohol, sealing places 24 h under the room temperature, carries out drawing-off in the deionized water more than 60 ℃, with one times of as-spun fibre drawing-off, in 120 ℃ of lower heat setting 30 min.The spun filament cellulose fiber is positioned over EDC/NHS(wt/wt=2/1) join by a certain percentage ethanol/water (V
C2H5OH/ V
H2O=90/10) mass fraction that is mixed with in is 24 h in 4.5% the crosslinked fluid, and drawing-off in the deionized water more than 60 ℃ is placed on 120 ℃ of lower heat setting 30 min, obtains the Regenerated Bombyx mori Silk Fibroin long filament of EDC/NHS after crosslinked;
(3) be 5% silk fibroin water solution with natural mulberry silk through coming unstuck, form after the dissolving, dialysis treatment mass fraction, aqueous solution is poured on the vinyon flat board, drying obtains fibroin membrane at ambient temperature; Regenerated Silk Fibroin Film is dissolved among the HFIP, and to make mass fraction one week be 10% spinning liquid in vibration in 25 ℃ of water-baths of constant temperature.Spinning liquid poured into carry out electrostatic spinning in the syringe, No. 9 syringe needles, spinning flow 2mL/h adjusts voltage and solidifies distance, makes field intensity reach 1kV/cm, makes regeneration mulberry silk nano-fibre yams;
(4) prepared Regenerated Bombyx mori Silk Fibroin long filament in fortifying fibre carbon fiber and the step (2) (3) and regeneration mulberry silk nano-fibre yams are inweaved the natural mulberry silk of step (1) preparation in compiling timbering material as stuffer weft, the common artificial ligament repair materials that makes up, adopt again serger that the ligament material both sides are sewed up and form column structure, the overall length 29cm of artificial ligament repair materials, parallel fiber zone length 3cm, two ends woollen yarn knitting zone length respectively is 10cm, the consolidation zone length of field is 3cm, and diameter is 10mm; The ultimate strength of this artificial ligament repair materials is 1250N.
Referring to accompanying drawing 1, Fig. 2, Fig. 3 and Fig. 4, be 29cm by the prepared artificial ligament repair materials of the present embodiment total length, parallel fiber zone length 3cm, two ends woollen yarn knitting zone length respectively is 10cm, and the consolidation zone length of field is 3cm, and diameter is 10mm; The average diameter of Regenerated Bombyx mori Silk Fibroin long filament is about 12 μ m; The diameter of regeneration mulberry silk nano-fibre yams is 280nm.
Embodiment two:
(1) braiding through compiling timbering material is with embodiment one;
(2) be 5% silk fibroin water solution with natural mulberry silk through coming unstuck, form after the dissolving, dialysis treatment mass fraction, it is positioned over stirring at low speed in the PEG solution, and to be concentrated into mass fraction be 27% silk fibroin water solution; Be about 25 ℃ in temperature, within the resting period of 3d, carry out wet spinning, the spun filament cellulose fiber is immersed in the dehydrated alcohol, sealing places 24 h under the room temperature, carries out drawing-off in the deionized water more than 60 ℃, with one times of as-spun fibre drawing-off, in 120 ℃ of lower heat setting 30 min.The spun filament cellulose fiber is positioned over EDC/NHS(wt/wt=2/1) join by a certain percentage ethanol/water (V
C2H5OH/ V
H2O=90/10) mass fraction that is mixed with in is 24 h in 4.5% the crosslinked fluid, and drawing-off in the deionized water more than 60 ℃ is placed on 120 ℃ of lower heat setting 30 min, obtains the Regenerated Bombyx mori Silk Fibroin long filament of EDC/NHS after crosslinked;
(3) be 7% silk fibroin water solution with natural mulberry silk through coming unstuck, form after the dissolving, dialysis treatment mass fraction, aqueous solution is poured on the vinyon flat board, at room temperature drying obtains fibroin membrane; Regenerated Silk Fibroin Film is dissolved in the formic acid, and to make mass fraction one week be 11% spinning liquid in vibration in 25 ℃ of water-baths of constant temperature.Spinning liquid poured into carry out electrostatic spinning in the syringe, No. 9 syringe needles, spinning flow 2mL/h adjusts voltage and solidifies distance, makes field intensity reach 1kV/cm, makes regeneration mulberry silk nano-fiber material;
(4) prepared regenerated silk fibroin long filament in fortifying fibre superhigh molecular weight polyethylene fibers and the step (2) (3) and regeneration mulberry silk nano-fibre yams are inweaved the natural mulberry silk of step (1) preparation in compiling timbering material as stuffer weft, jointly make up the artificial ligament repair materials, further improve the mechanical property of material.Adopt again serger that the ligament material both sides are sewed up and form column structure, the overall length 27.5cm of artificial ligament repair materials, parallel fiber zone length 1.5cm, two ends woollen yarn knitting zone length respectively is 10cm, and the consolidation zone length of field is 3cm, and diameter is 6mm.
Claims (10)
1. a fibroin base artificial ligament repair materials comprises support and stuffer weft, it is characterized in that: described support adopts twin shaft to consist of through volume to inlaid thread by natural mulberry silk; Stuffer weft is Regenerated Bombyx mori Silk Fibroin long filament and regeneration mulberry silk nano-fibre yams, and described stuffer weft inweaves in the warp knitting fabrics structure of support.
2. artificial ligament repair materials according to claim 1, it is characterized in that: the natural mulberry silk consumption accounts for 30～70% of artificial ligament repair materials total amount in the described artificial ligament repair materials; Regenerated Bombyx mori Silk Fibroin long filament consumption accounts for 10～30% of artificial ligament repair materials total amount; Regeneration mulberry silk nano-fibre yams consumption accounts for 20～40% of artificial ligament repair materials total amount.
3. artificial ligament repair materials according to claim 1, it is characterized in that: described artificial ligament repair materials length is 27.5～41cm; The diameter of described Regenerated Bombyx mori Silk Fibroin long filament is 15～80 μ m; The diameter of described regeneration mulberry silk nano-fibre yams is 50～2000nm.
4. artificial ligament repair materials according to claim 1, it is characterized in that: described artificial ligament repair materials also comprises reinforcing fibre.
5. artificial ligament repair materials according to claim 4, it is characterized in that: the reinforcing fibre consumption accounts for 10～90% of the total consumption of stuffer weft in the described artificial ligament repair materials.
6. artificial ligament repair materials according to claim 4, it is characterized in that: described reinforcing fibre is carbon fiber, supermolecule weight polyethylene fiber or polyester fiber.
7. a method for preparing artificial ligament repair materials claimed in claim 1 is characterized in that, may further comprise the steps:
(1) adopt natural mulberry silk braiding twin shaft to inlaid thread through compiling timbering material, after obtain Bombyx mori Silk Fibroin through compiling timbering material through coming unstuck;
(2) natural mulberry silk prepares regenerated silk fibroin solution after coming unstuck, dissolve, dialysing, and adopts dry method or wet spinning to prepare the Regenerated Bombyx mori Silk Fibroin long filament;
(3) natural mulberry silk uses hexafluoroisopropanol or formic acid dissolving fibroin protein film through coming unstuck, preparing regenerated silk fibroin film after the dissolving, dialysis, drying, then adopts method of electrostatic spinning preparation regeneration mulberry silk nano-fibre yams;
(4) take fibroin through compiling timbering material as base material, Regenerated Bombyx mori Silk Fibroin long filament and regeneration mulberry silk nano-fibre yams inweave in the warp knitting fabrics structure as stuffer weft, reeling forms the artificial ligament material, the ligament material both sides is sewed up namely got described fibroin base artificial ligament repair materials at last.
8. preparation method according to claim 7, it is characterized in that: in the described step (4), stuffer weft also comprises carbon fiber, superhigh molecular weight polyethylene fibers or polyester fiber.
9. preparation method according to claim 7 is characterized in that: the regeneration Bombyx mori Silk Fibroin long filament of preparation also passes through 1-(3-dimethylaminopropyl)-3-ethyl carbodiimides and N-maloyl imines crosslinking Treatment in the described step (2).
10. preparation method according to claim 7 is characterized in that: in mass ratio, natural mulberry silk: Regenerated Bombyx mori Silk Fibroin long filament: regeneration mulberry silk nano-fibre yams is (30～70): (10～30): (20～40).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310284212.2A CN103357067B (en) | 2013-07-08 | 2013-07-08 | Fibroin-base artificial ligament repair material and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310284212.2A CN103357067B (en) | 2013-07-08 | 2013-07-08 | Fibroin-base artificial ligament repair material and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103357067A true CN103357067A (en) | 2013-10-23 |
| CN103357067B CN103357067B (en) | 2014-07-16 |
Family
ID=49359859
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310284212.2A Active CN103357067B (en) | 2013-07-08 | 2013-07-08 | Fibroin-base artificial ligament repair material and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103357067B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105970347A (en) * | 2016-05-27 | 2016-09-28 | 东莞市联洲知识产权运营管理有限公司 | Low temperature sensitive shape memory fiber and preparing method thereof |
| CN106048765A (en) * | 2016-07-14 | 2016-10-26 | 苏州经贸职业技术学院 | Preparation method of artificial ligament material |
| CN106730016A (en) * | 2016-12-09 | 2017-05-31 | 苏州纳贝通环境科技有限公司 | A kind of activity modifying modifying artificial ligament and its preparation technology |
| CN106929997A (en) * | 2015-12-31 | 2017-07-07 | 苏州微创脊柱创伤医疗科技有限公司 | A kind of artificial soft tissue's braid and preparation method thereof |
| CN106975104A (en) * | 2016-01-19 | 2017-07-25 | 苏州微创脊柱创伤医疗科技有限公司 | A kind of artificial ligament braid and its production and use |
| CN110331486A (en) * | 2019-07-25 | 2019-10-15 | 东华大学 | A kind of multilayered structure nano-fibre yams knitting tendon scaffold and its preparation and application |
| CN110947032A (en) * | 2019-12-16 | 2020-04-03 | 浙江大学 | Silk/PET mixed-woven bracket and preparation method and application thereof |
| CN113663128A (en) * | 2021-09-29 | 2021-11-19 | 东华大学 | High-strength composite coating artificial ligament and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1410811A1 (en) * | 2002-10-18 | 2004-04-21 | Ethicon, Inc. | Biocompatible scaffolds with tissue fragments |
| CN101209357A (en) * | 2007-12-21 | 2008-07-02 | 南京大学医学院附属鼓楼医院 | Method for preparing medical knee joint cruciate ligaments |
| EP2210971A1 (en) * | 2009-01-26 | 2010-07-28 | Politecnico Di Milano | Silk fibroin textile structures as biomimetic prosthetics for the regeneration of tissues and ligaments |
| CN102107021A (en) * | 2010-08-10 | 2011-06-29 | 贾庆卫 | Silk ligament and preparation method thereof |
| US20130073055A1 (en) * | 2010-03-17 | 2013-03-21 | Dongguk University Industry-Academic Cooperation Foundation | Composite support containing silk and collagen, and preparation method thereof |
-
2013
- 2013-07-08 CN CN201310284212.2A patent/CN103357067B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1410811A1 (en) * | 2002-10-18 | 2004-04-21 | Ethicon, Inc. | Biocompatible scaffolds with tissue fragments |
| CN101209357A (en) * | 2007-12-21 | 2008-07-02 | 南京大学医学院附属鼓楼医院 | Method for preparing medical knee joint cruciate ligaments |
| EP2210971A1 (en) * | 2009-01-26 | 2010-07-28 | Politecnico Di Milano | Silk fibroin textile structures as biomimetic prosthetics for the regeneration of tissues and ligaments |
| US20130073055A1 (en) * | 2010-03-17 | 2013-03-21 | Dongguk University Industry-Academic Cooperation Foundation | Composite support containing silk and collagen, and preparation method thereof |
| CN102107021A (en) * | 2010-08-10 | 2011-06-29 | 贾庆卫 | Silk ligament and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| BANANI KUNDU ETAL.: ""Silk fibroin biomaterials for tissue regenerations"", 《ADVANCED DRUG DELIVERY REVIEWS》 * |
| XIAO CHEN ETAL.: ""Ligament regeneration using a knitted silk scaffold combined with collagen matrix"", 《BIOMATERIALS》 * |
| 吴惠英，左保齐: ""医用支架型前交叉韧带的设计"", 《针织工业》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106929997A (en) * | 2015-12-31 | 2017-07-07 | 苏州微创脊柱创伤医疗科技有限公司 | A kind of artificial soft tissue's braid and preparation method thereof |
| CN106975104A (en) * | 2016-01-19 | 2017-07-25 | 苏州微创脊柱创伤医疗科技有限公司 | A kind of artificial ligament braid and its production and use |
| CN105970347A (en) * | 2016-05-27 | 2016-09-28 | 东莞市联洲知识产权运营管理有限公司 | Low temperature sensitive shape memory fiber and preparing method thereof |
| CN106048765A (en) * | 2016-07-14 | 2016-10-26 | 苏州经贸职业技术学院 | Preparation method of artificial ligament material |
| CN106048765B (en) * | 2016-07-14 | 2018-05-08 | 苏州经贸职业技术学院 | A kind of preparation method of artificial ligament material |
| CN106730016A (en) * | 2016-12-09 | 2017-05-31 | 苏州纳贝通环境科技有限公司 | A kind of activity modifying modifying artificial ligament and its preparation technology |
| CN110331486A (en) * | 2019-07-25 | 2019-10-15 | 东华大学 | A kind of multilayered structure nano-fibre yams knitting tendon scaffold and its preparation and application |
| CN110947032A (en) * | 2019-12-16 | 2020-04-03 | 浙江大学 | Silk/PET mixed-woven bracket and preparation method and application thereof |
| CN113663128A (en) * | 2021-09-29 | 2021-11-19 | 东华大学 | High-strength composite coating artificial ligament and preparation method and application thereof |
| CN113663128B (en) * | 2021-09-29 | 2022-05-10 | 东华大学 | High-strength composite coating artificial ligament and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103357067B (en) | 2014-07-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103357067B (en) | Fibroin-base artificial ligament repair material and preparation method thereof | |
| Wu et al. | State-of-the-art review of advanced electrospun nanofiber yarn-based textiles for biomedical applications | |
| CN101214393B (en) | Nano fibrous tissue engineering blood vessel and preparation thereof | |
| US10758641B2 (en) | Process for the production of a hybrid structure consisting of coupled silk fibroin microfibers and nanofibers, hybrid structure thus obtained and its use as implantable medical device | |
| CN105363076B (en) | A kind of preparation method of polylactic acid caprolactone-collagen double-layer bionic intravascular stent | |
| CN100368610C (en) | Blending nanometer fibre with silk protein and vinol and production thereof | |
| AU5169398A (en) | Implantable fibers and medical articles | |
| CN103154347A (en) | Hydrogel fibres and fibrous structures | |
| CN104043151A (en) | Composite artificial ligament and preparation method thereof | |
| CN103320886A (en) | Bionic regenerated silk fibroin filament fiber and preparation method thereof | |
| McCullen et al. | Fiber-reinforced scaffolds for tissue engineering and regenerative medicine: use of traditional textile substrates to nanofibrous arrays | |
| Gupta | Manufacture, types and properties of biotextiles for medical applications | |
| CN105457096A (en) | Preparation methods of degradable tussah fibroin tissue engineering scaffold material with good biocompatibility | |
| CN107929803A (en) | A kind of nano-fibre yams surgical thread and preparation method thereof | |
| KR101035870B1 (en) | Absorbable bulky multi-filament draw textured yarn, manufacturing method thereof and medical use using them | |
| CN106390208A (en) | Three-dimensional support material containing hierarchical porous structures and preparation and application | |
| WO2016202281A1 (en) | Reinforced electrospun fibrous membrane and preparation method thereof | |
| CN112972770A (en) | Artificial heart valve with composite reinforced structure and preparation method thereof | |
| KR20120131664A (en) | 3-dimensional porous scaffold and manufacturing method thereof | |
| Chang et al. | Medical fibers and biotextiles | |
| Doersam et al. | A Review: Textile Technologies for Single and Multi‐Layer Tubular Soft Tissue Engineering | |
| CN113388950A (en) | Absorbable high-performance nanofiber woven tendon patch and preparation method thereof | |
| CN101829356B (en) | Tissue engineering artificial tendon scaffold enhanced body and preparation method thereof | |
| CN112675363A (en) | 3D polylactic acid scaffold for bone tissue engineering and preparation method thereof | |
| King et al. | Structural design, fabrication and evaluation of resorbable fiber-based tissue engineering scaffolds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |